Rh3CG279400

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0013576
Physical Location & Seq
Forward (+)
28020653 .. 28093067
72415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG279400.1

Sequence Viewer

Length: 1419 bp
ATGGCGTGCAGTGCCTCAACTTGCCAGTCAAATTGCTACAGAGACCAACCACAAGAAGAAGAAGAGAAAGAAGATGCCAAACATACCAATACCAATTCAATTGCTACTAACACAATCACCAACAACAACAACAGAAACAACAGCCATGGTTTGTGCCTCAAGTGCAAGGCCAACCCTCCCATCTCCGCCGATACCCACGCCGACGATTCCCGTTTCTGCGCCGATTGCTTCCGCGCAAACCTCTTTGGCAAGTTCAGGTTCGCCGTCACCTCCAACGCCATGATCTCTCCCACTGATAACGTCCTCGTCGCCTTCTCCGGCGGCCCTTCTTCCAGGGTTGCTTTACAGTTTGTACATGAGATGCACAGTAAAGCACAGAAGAATTTTGATGCTAGTAGGGATAGATCGTTACCGGTGTTCGGTGTCGGAGTTGCATTTATTGATGAAACTAGTGTTTATTCTGTTCCATCCAATGAACAAGACAATGCAATTGCAGACATTACATCTATTGTGGAAAACCTAGCCCCGCCGATAAAACAATTGTACGTTGTACCGATTGAGAGTGTCTACTCTGAAGAGCCTGGCGATGGCAGGGAGAGACTGAAGAAGTTACTAGATGCTGTTACTGACGCCACTGGAAGAGAAGATCTTTTACTACATCTGCGGATGTTGGCCTTGCAAAAGATTGCCTCACAAAATGGATACAACAGACTTCTACTGGGATCATGCATATCCAGGATTGCTTGCCATGTTATTACAGCCACTGTGAAGGGACAAGGATATTCACTACCAGCAGATATACAGTATGTTGATTCAAGGTGGGAAATCCCAGTTTTGCTTCCTCTTCATGATTGTCTTGCACAAGAGCTGAACATGCTTTGCTGCCTTGATGGTCTAAAAACTGTTGAGTTGATAAGAAGTCCTGGCACTGGCATTAATGGGTTGGTTTCATCATTTGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGGGAGTGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCGAGAGATCGATGACTCTAATGTCCCTTTGGCAACTCGAAGGCGTCAGAAGAGATATAACCTTAAACCTAATGAATGTTTTTCTTCAGAGTCGTTCTGCTTCATCTGCAATGGCCCACTCAGCAAATCTGATTTGCTAAGTTTGCGCAATCTTGAACAATGCCAAACAAGTCCCAACACTTTGGGTGGTGTCTGCTGTTCGAGCTGCTGGTTTCAGATAATTCCCCAGGACCCTTCATTACTGGATCAATTTTCTGCAGTTTTACCTCAGCAGCTGGTCGCTCGGGCAAAGCATGACAGCCTTGACAATTACAGTGCGCTCAGGGAACAAATACAAGATTGCTTGCTATCAGAAGGCGAAGACGAAACCTGA

Protein Analysis

472

Amino Acids

52.2

Weight (kDa)

5.4

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1067
Acc16I TGCGCA 1 cut(s) 1193
AccI GTMKAC 1 cut(s) 567
AccII CGCG 1 cut(s) 234
AciI CCGC 5 cut(s) 186, 232, 321, 527, 664
AclWI GGATC 2 cut(s) 730, 1299
AcsI RAATTY 2 cut(s) 382, 1044
AcuI CTGAAG 3 cut(s) 594, 623, 1116
AcyI GRCGYC 2 cut(s) 630, 1090
AfaI GTAC 3 cut(s) 354, 545, 552
AfiI CCNNNNNNNGG 3 cut(s) 419, 587, 929
AgeI ACCGGT 1 cut(s) 412
AgsI TTSAA 4 cut(s) 99, 816, 1039, 1202
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 5 cut(s) 332, 580, 734, 922, 1272
AleI CACNNNNGTG 1 cut(s) 1001
AluBI AGCT 5 cut(s) 868, 1013, 1021, 1251, 1321
AluI AGCT 5 cut(s) 868, 1013, 1021, 1251, 1321
Alw21I GWGCWC 1 cut(s) 998
Alw26I GTCTC 2 cut(s) 36, 592
Alw44I GTGCAC 1 cut(s) 994
AlwI GGATC 2 cut(s) 730, 1299
AlwNI CAGNNNCTG 2 cut(s) 764, 1321
Ama87I CYCGRG 2 cut(s) 987, 1329
AoxI GGCC 4 cut(s) 168, 322, 672, 1159
ApaLI GTGCAC 1 cut(s) 994
ApeKI GCWGC 3 cut(s) 882, 1251, 1318
ApoI RAATTY 2 cut(s) 382, 1044
AseI ATTAAT 1 cut(s) 936
AsiGI ACCGGT 1 cut(s) 412
AspLEI GCGC 4 cut(s) 221, 236, 1194, 1366
AspS9I GGNCC 3 cut(s) 323, 1160, 1276
AsuHPI GGTGA 2 cut(s) 109, 259
AvaI CYCGRG 2 cut(s) 987, 1329
AvaII GGWCC 1 cut(s) 1276
BaeGI GKGCMC 1 cut(s) 998
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
BbsI GAAGAC 1 cut(s) 1413
Bbv12I GWGCWC 1 cut(s) 998
BbvCI CCTCAGC 1 cut(s) 1314
BbvI GCAGC 3 cut(s) 869, 1238, 1330
BccI CCATC 4 cut(s) 188, 475, 581, 884
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 5 cut(s) 334, 582, 736, 924, 1274
BciVI GTATCC 1 cut(s) 695
BcoDI GTCTC 2 cut(s) 36, 592
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 4 cut(s) 393, 450, 521, 614
BfmI CTRYAG 2 cut(s) 37, 1302
BfuI GTATCC 1 cut(s) 695
BglII AGATCT 1 cut(s) 646
BisI GCNGC 4 cut(s) 322, 883, 1252, 1319
BlsI GCNGC 4 cut(s) 323, 884, 1253, 1320
Bme1390I CCNGG 5 cut(s) 334, 582, 736, 924, 1274
Bme18I GGWCC 1 cut(s) 1276
BmeT110I CYCGRG 2 cut(s) 987, 1329
BmgT120I GGNCC 3 cut(s) 323, 1160, 1276
BmiI GGNNCC 1 cut(s) 1278
BmrFI CCNGG 5 cut(s) 334, 582, 736, 924, 1274
BmrI ACTGGG 2 cut(s) 728, 824
BmsI GCATC 4 cut(s) 64, 351, 379, 607
BmuI ACTGGG 2 cut(s) 728, 824
BpiI GAAGAC 1 cut(s) 1413
Bpu10I CCTNAGC 2 cut(s) 1314, 1367
BpuEI CTTGAG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 1056
BsaHI GRCGYC 2 cut(s) 630, 1090
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 145, 333, 1272
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 587, 929
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 6 cut(s) 25, 640, 723, 830, 934, 1293
Bse3DI GCAATG 1 cut(s) 1162
BseBI CCWGG 5 cut(s) 334, 582, 736, 924, 1274
BseCI ATCGAT 1 cut(s) 1056
BseDI CCNNGG 3 cut(s) 145, 333, 1272
BseGI GGATG 2 cut(s) 467, 672
BseLI CCNNNNNNNGG 3 cut(s) 419, 587, 929
BseMI GCAATG 1 cut(s) 1162
BseMII CTCAG 3 cut(s) 1180, 1328, 1381
BseNI ACTGG 6 cut(s) 25, 640, 723, 830, 934, 1293
BseSI GKGCMC 1 cut(s) 998
BseXI GCAGC 3 cut(s) 869, 1238, 1330
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 4 cut(s) 170, 324, 674, 1161
BshTI ACCGGT 1 cut(s) 412
BshVI ATCGAT 1 cut(s) 1056
BsiHKAI GWGCWC 1 cut(s) 998
BsiHKCI CYCGRG 2 cut(s) 987, 1329
BsiSI CCGG 2 cut(s) 318, 413
BslFI GGGAC 3 cut(s) 786, 1055, 1203
BslI CCNNNNNNNGG 3 cut(s) 419, 587, 929
BsmAI GTCTC 2 cut(s) 36, 592
BsmFI GGGAC 3 cut(s) 786, 1055, 1203
BsnI GGCC 4 cut(s) 170, 324, 674, 1161
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 2 cut(s) 987, 1329
Bsp1286I GDGCHC 1 cut(s) 998
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 5 cut(s) 186, 232, 321, 527, 664
BspANI GGCC 4 cut(s) 170, 324, 674, 1161
BspCNI CTCAG 3 cut(s) 1179, 1327, 1380
BspDI ATCGAT 1 cut(s) 1056
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 1 cut(s) 1278
BspMAI CTGCAG 1 cut(s) 1306
BspPI GGATC 2 cut(s) 730, 1299
BspQI GCTCTTC 1 cut(s) 570
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 1162
BsrFI RCCGGY 1 cut(s) 412
BsrGI TGTACA 1 cut(s) 352
BsrI ACTGG 6 cut(s) 25, 640, 723, 830, 934, 1293
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 3 cut(s) 145, 333, 1272
BssMI GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
BssNI GRCGYC 2 cut(s) 630, 1090
BssT1I CCWWGG 1 cut(s) 145
Bst2UI CCWGG 5 cut(s) 334, 582, 736, 924, 1274
Bst4CI ACNGT 6 cut(s) 348, 368, 766, 804, 904, 1361
Bst6I CTCTTC 5 cut(s) 57, 570, 634, 849, 1091
BstACI GRCGYC 2 cut(s) 630, 1090
BstAUI TGTACA 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 7, 745, 1391
BstDEI CTNAG 4 cut(s) 1166, 1184, 1314, 1367
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 2 cut(s) 467, 672
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 4 cut(s) 221, 236, 1194, 1366
BstKTI GATC 6 cut(s) 285, 407, 649, 725, 1056, 1294
BstMAI GTCTC 2 cut(s) 36, 592
BstMBI GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
BstMWI GCNNNNNNNGC 8 cut(s) 11, 162, 225, 874, 1152, 1167, 1189, 1248
BstNI CCWGG 5 cut(s) 334, 582, 736, 924, 1274
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 5 cut(s) 332, 580, 734, 922, 1272
BstSFI CTRYAG 2 cut(s) 37, 1302
BstSLI GKGCMC 1 cut(s) 998
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 869, 1238, 1330
BstV2I GAAGAC 1 cut(s) 1413
BstX2I RGATCY 1 cut(s) 646
BstXI CCANNNNNNTGG 1 cut(s) 1228
BstYI RGATCY 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 1056
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 4 cut(s) 170, 324, 674, 1161
BsuTUI ATCGAT 1 cut(s) 1056
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 600
BtsCI GGATG 2 cut(s) 467, 672
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 6 cut(s) 16, 291, 633, 762, 927, 1366
Cac8I GCNNGC 3 cut(s) 7, 745, 1391
CaiI CAGNNNCTG 2 cut(s) 764, 1321
CciI TCATGA 1 cut(s) 847
CfoI GCGC 4 cut(s) 221, 236, 1194, 1366
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 323, 1160, 1276
ClaI ATCGAT 1 cut(s) 1056
CseI GACGC 2 cut(s) 638, 1079
Csp6I GTAC 3 cut(s) 353, 544, 551
CspAI ACCGGT 1 cut(s) 412
CviAII CATG 8 cut(s) 146, 280, 356, 726, 749, 848, 874, 1340
CviQI GTAC 3 cut(s) 353, 544, 551
DdeI CTNAG 4 cut(s) 1166, 1184, 1314, 1367
DpnI GATC 6 cut(s) 284, 406, 648, 724, 1055, 1293
DpnII GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
DrdI GACNNNNNNGTC 1 cut(s) 1067
DseDI GACNNNNNNGTC 1 cut(s) 1067
Eam1104I CTCTTC 5 cut(s) 57, 570, 634, 849, 1091
EarI CTCTTC 5 cut(s) 57, 570, 634, 849, 1091
EciI GGCGGA 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 594, 623, 1116
Eco88I CYCGRG 2 cut(s) 987, 1329
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRI GAATTC 1 cut(s) 1044
EcoRII CCWGG 5 cut(s) 332, 580, 734, 922, 1272
EcoT14I CCWWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 8 cut(s) 149, 283, 359, 729, 752, 851, 877, 1343
FaqI GGGAC 3 cut(s) 786, 1055, 1203
FatI CATG 8 cut(s) 145, 279, 355, 725, 748, 847, 873, 1339
FauI CCCGC 1 cut(s) 534
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 4 cut(s) 322, 883, 1252, 1319
FokI GGATG 2 cut(s) 454, 679
Fsp4HI GCNGC 4 cut(s) 322, 883, 1252, 1319
FspBI CTAG 4 cut(s) 393, 450, 521, 614
FspI TGCGCA 1 cut(s) 1193
GlaI GCGC 4 cut(s) 220, 235, 1193, 1365
GluI GCNGC 4 cut(s) 322, 883, 1252, 1319
HaeIII GGCC 4 cut(s) 170, 324, 674, 1161
HapII CCGG 2 cut(s) 318, 413
HgaI GACGC 2 cut(s) 638, 1079
HhaI GCGC 4 cut(s) 221, 236, 1194, 1366
Hin1I GRCGYC 2 cut(s) 630, 1090
Hin1II CATG 8 cut(s) 149, 283, 359, 729, 752, 851, 877, 1343
Hin6I GCGC 4 cut(s) 219, 234, 1192, 1364
HinP1I GCGC 4 cut(s) 219, 234, 1192, 1364
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 4 cut(s) 206, 812, 1061, 1136
HpaII CCGG 2 cut(s) 318, 413
HphI GGTGA 2 cut(s) 109, 259
Hpy166II GTNNAC 2 cut(s) 568, 996
Hpy188I TCNGA 7 cut(s) 428, 574, 1095, 1135, 1177, 1263, 1399
Hpy188III TCNNGA 4 cut(s) 848, 989, 1049, 1199
Hpy8I GTNNAC 2 cut(s) 568, 996
Hpy99I CGWCG 2 cut(s) 206, 311
HpyAV CCTTC 7 cut(s) 322, 336, 763, 993, 1080, 1290, 1394
HpyCH4III ACNGT 6 cut(s) 348, 368, 766, 804, 904, 1361
HpyCH4IV ACGT 2 cut(s) 300, 546
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 162, 225, 874, 1152, 1167, 1189, 1248
HpyF3I CTNAG 4 cut(s) 1166, 1184, 1314, 1367
HpySE526I ACGT 2 cut(s) 300, 546
Hsp92I GRCGYC 2 cut(s) 630, 1090
Hsp92II CATG 8 cut(s) 149, 283, 359, 729, 752, 851, 877, 1343
HspAI GCGC 4 cut(s) 219, 234, 1192, 1364
Kzo9I GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
LguI GCTCTTC 1 cut(s) 570
Lsp1109I GCAGC 3 cut(s) 869, 1238, 1330
LweI GCATC 4 cut(s) 64, 351, 379, 607
MaeI CTAG 4 cut(s) 393, 450, 521, 614
MaeII ACGT 2 cut(s) 300, 546
MaeIII GTNAC 5 cut(s) 265, 408, 609, 622, 960
MalI GATC 6 cut(s) 284, 406, 648, 724, 1055, 1293
MboI GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
MfeI CAATTG 4 cut(s) 99, 489, 539, 999
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 1 cut(s) 998
MlyI GAGTC 2 cut(s) 1055, 1145
MmeI TCCRAC 2 cut(s) 297, 406
MnlI CCTC 9 cut(s) 25, 167, 186, 251, 280, 314, 700, 852, 1323
Mph1103I ATGCAT 1 cut(s) 731
MseI TTAA 2 cut(s) 936, 1110
MslI CAYNNNNRTG 1 cut(s) 1001
MspA1I CMGCKG 2 cut(s) 1013, 1321
MspI CCGG 2 cut(s) 318, 413
MspR9I CCNGG 5 cut(s) 334, 582, 736, 924, 1274
MunI CAATTG 4 cut(s) 99, 489, 539, 999
MvaI CCWGG 5 cut(s) 334, 582, 736, 924, 1274
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 8 cut(s) 11, 162, 225, 874, 1152, 1167, 1189, 1248
NcoI CCATGG 1 cut(s) 145
NdeII GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
NlaIII CATG 8 cut(s) 149, 283, 359, 729, 752, 851, 877, 1343
NlaIV GGNNCC 1 cut(s) 1278
NmuCI GTSAC 2 cut(s) 265, 960
NsbI TGCGCA 1 cut(s) 1193
NsiI ATGCAT 1 cut(s) 731
NspI RCATGY 1 cut(s) 877
OliI CACNNNNGTG 1 cut(s) 1001
PagI TCATGA 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 206, 812
PfoI TCCNGGA 1 cut(s) 734
PinAI ACCGGT 1 cut(s) 412
PkrI GCNGC 4 cut(s) 323, 884, 1253, 1320
PleI GAGTC 2 cut(s) 1055, 1144
PpsI GAGTC 2 cut(s) 1055, 1144
PpuMI RGGWCCY 1 cut(s) 1276
PshBI ATTAAT 1 cut(s) 936
Psp5II RGGWCCY 1 cut(s) 1276
Psp6I CCWGG 5 cut(s) 332, 580, 734, 922, 1272
PspGI CCWGG 5 cut(s) 332, 580, 734, 922, 1272
PspN4I GGNNCC 1 cut(s) 1278
PspPI GGNCC 3 cut(s) 323, 1160, 1276
PspPPI RGGWCCY 1 cut(s) 1276
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 2 cut(s) 764, 1321
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 2 cut(s) 1013, 1321
RsaI GTAC 3 cut(s) 354, 545, 552
RsaNI GTAC 3 cut(s) 353, 544, 551
RseI CAYNNNNRTG 1 cut(s) 1001
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 2 cut(s) 936, 1110
SatI GCNGC 4 cut(s) 322, 883, 1252, 1319
Sau3AI GATC 6 cut(s) 282, 404, 646, 722, 1053, 1291
Sau96I GGNCC 3 cut(s) 323, 1160, 1276
SchI GAGTC 2 cut(s) 1055, 1145
ScrFI CCNGG 5 cut(s) 334, 582, 736, 924, 1274
SduI GDGCHC 1 cut(s) 998
SfaNI GCATC 4 cut(s) 64, 351, 379, 607
SfcI CTRYAG 2 cut(s) 37, 1302
SinI GGWCC 1 cut(s) 1276
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 449
SsiI CCGC 5 cut(s) 186, 232, 321, 527, 664
SspMI CTAG 4 cut(s) 393, 450, 521, 614
StyD4I CCNGG 5 cut(s) 332, 580, 734, 922, 1272
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 6 cut(s) 348, 368, 766, 804, 904, 1361
TaiI ACGT 2 cut(s) 303, 549
TaqI TCGA 4 cut(s) 1048, 1056, 1084, 1247
TatI WGTACW 1 cut(s) 352
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 2 cut(s) 206, 812
Tru1I TTAA 2 cut(s) 936, 1110
Tru9I TTAA 2 cut(s) 936, 1110
TscAI CASTG 6 cut(s) 16, 298, 640, 769, 934, 1366
TseFI GTSAC 2 cut(s) 265, 960
TseI GCWGC 3 cut(s) 882, 1251, 1318
Tsp45I GTSAC 2 cut(s) 265, 960
TspDTI ATGAA 8 cut(s) 459, 489, 836, 939, 1022, 1134, 1138, 1272
TspRI CASTG 6 cut(s) 16, 298, 640, 769, 934, 1366
VneI GTGCAC 1 cut(s) 994
VpaK11BI GGWCC 1 cut(s) 1276
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 2 cut(s) 382, 1044
XceI RCATGY 1 cut(s) 877
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 4 cut(s) 393, 450, 521, 614
Zsp2I ATGCAT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.