Rmu_sc0013521.1_g000004

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013521.1
OG0013576
Physical Location & Seq
Reverse (-)
9400 .. 13085
3686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013521.1_g000004.1.cds

Sequence Viewer

Length: 1410 bp
atggcttgcagtgcctcaacttgccaatcaagttgctacagagaccaaccacaagaagaagaagaagatgccaaacacaccaatgccaattcaattgctaccaacacaatcaccaacaacaacaacagaaacaacagccatggcttgtgcctcaagtgcaaggccaaccctcccatctccgccgatacccacgccgacgattcccgattctgcgccgattgcttccgcgcaaacctcttcggcaagttcaggttcgccgtcacctccaactccatgatttctcccactgataacgtcctcgtcgccttctccggcggcccttcttccagggttgctttagagtttgtacatgagatgcacagtaaagcgcagaagaattttgatgctagtagggatagatcgttaccggtgttcggtgttggagttgcatttattgatgaaactagtgtttattctgttccatccaatgaacaagacaatgcaattgaagagattacatctattgtggaaagcctagccccgccgacaaaacagttgtatgttgtgccgattgagagtgtctactctgaagagcctggcgatggcagggagagactgaagaagttactagatgctgttactgacgccactggaagagaagatcttttactacatctgcggatgttggccttgcaaaagattgcctcacaaaatggatacaacagacttcttctgggatcatgcatatcgaggattgcttgccatgttattacagccactgtgaagggacaaggatattcactaccagcagatatacagtatgttgattcaaggtgggaaatcccagttttgcttcctcttcgtgattgtcttgcacaagagctgaacatgctttgccgccttgatggtctaaaaactgttgagttgataagaagtcctggcactggcattaatggtttggtttcatcatttgtgtcactgttgcaggaagaaaatccttctcgggagtgcacaattgtgagaacagctggaaagcttatcccttttcatttcaacagaattcaagagatcgatgactctaatgtccctttggcaactcgaaggcgtcagaagagatataaccttaaacctaatgaatgtttttcttcagagtcattctgcttcatctgcaatgccccactcagcaaatctgatttgctaagtttgcgcaatcttgaacaatgccaaacaagtcccgacacttttggtagtgcctgctgttcgagctgccggtttcagatacttccccaggacccttcattactggatcaattttctgcacttttacctcagcaggtcgctcgggcaaaacatgacaaccttgacaattacagtgtgctcagggaacaaatacaagattgcttgctttcggaaagcgaagatgaaacctga

Protein Analysis

469

Amino Acids

51.99

Weight (kDa)

5.28

Isoelectric Point (pI)

58.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1061
Acc16I TGCGCA 1 cut(s) 1187
Acc36I ACCTGC 1 cut(s) 1303
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 228
AciI CCGC 6 cut(s) 180, 226, 315, 521, 658, 877
AclWI GGATC 2 cut(s) 724, 1293
AcsI RAATTY 2 cut(s) 376, 1038
AcuI CTGAAG 3 cut(s) 588, 617, 1110
AcyI GRCGYC 2 cut(s) 624, 1084
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 3 cut(s) 413, 581, 923
AgeI ACCGGT 1 cut(s) 406
AgsI TTSAA 6 cut(s) 93, 488, 810, 1033, 1043, 1196
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 4 cut(s) 326, 574, 916, 1266
AleI CACNNNNGTG 1 cut(s) 995
AluBI AGCT 4 cut(s) 862, 1007, 1015, 1245
AluI AGCT 4 cut(s) 862, 1007, 1015, 1245
Alw21I GWGCWC 2 cut(s) 992, 1359
Alw26I GTCTC 2 cut(s) 36, 586
Alw44I GTGCAC 1 cut(s) 988
AlwI GGATC 2 cut(s) 724, 1293
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 2 cut(s) 981, 1320
AoxI GGCC 3 cut(s) 162, 316, 666
ApaLI GTGCAC 1 cut(s) 988
ApeKI GCWGC 1 cut(s) 1245
ApoI RAATTY 2 cut(s) 376, 1038
AseI ATTAAT 1 cut(s) 930
AsiGI ACCGGT 1 cut(s) 406
AspLEI GCGC 4 cut(s) 215, 230, 370, 1188
AspS9I GGNCC 2 cut(s) 317, 1270
AsuHPI GGTGA 2 cut(s) 103, 253
AvaI CYCGRG 2 cut(s) 981, 1320
AvaII GGWCC 1 cut(s) 1270
BaeGI GKGCMC 1 cut(s) 992
BarI GAAGNNNNNNTAC 2 cut(s) 630, 662
Bbv12I GWGCWC 2 cut(s) 992, 1359
BbvCI CCTCAGC 1 cut(s) 1308
BbvI GCAGC 1 cut(s) 1232
BccI CCATC 4 cut(s) 182, 469, 575, 878
BceAI ACGGC 1 cut(s) 242
BcgI CGANNNNNNTGC 2 cut(s) 1301, 1335
BciT130I CCWGG 4 cut(s) 328, 576, 918, 1268
BciVI GTATCC 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 36, 586
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 4 cut(s) 387, 444, 515, 608
BfmI CTRYAG 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 1 cut(s) 689
BglII AGATCT 1 cut(s) 640
BisI GCNGC 3 cut(s) 316, 877, 1246
BlsI GCNGC 3 cut(s) 317, 878, 1247
Bme1390I CCNGG 4 cut(s) 328, 576, 918, 1268
Bme18I GGWCC 1 cut(s) 1270
BmeT110I CYCGRG 2 cut(s) 981, 1320
BmgT120I GGNCC 2 cut(s) 317, 1270
BmiI GGNNCC 1 cut(s) 1272
BmrFI CCNGG 4 cut(s) 328, 576, 918, 1268
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 4 cut(s) 58, 345, 373, 601
BmuI ACTGGG 1 cut(s) 818
Bpu10I CCTNAGC 2 cut(s) 1308, 1358
BpuEI CTTGAG 1 cut(s) 137
Bsa29I ATCGAT 1 cut(s) 1050
BsaHI GRCGYC 2 cut(s) 624, 1084
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 139, 327, 1266
BsaWI WCCGGW 1 cut(s) 406
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 581, 923
Bse118I RCCGGY 2 cut(s) 406, 1248
Bse1I ACTGG 4 cut(s) 634, 824, 928, 1287
Bse3DI GCAATG 1 cut(s) 1156
BseBI CCWGG 4 cut(s) 328, 576, 918, 1268
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 3 cut(s) 139, 327, 1266
BseGI GGATG 2 cut(s) 461, 666
BseLI CCNNNNNNNGG 3 cut(s) 413, 581, 923
BseMI GCAATG 1 cut(s) 1156
BseMII CTCAG 3 cut(s) 1174, 1322, 1372
BseNI ACTGG 4 cut(s) 634, 824, 928, 1287
BseSI GKGCMC 1 cut(s) 992
BseXI GCAGC 1 cut(s) 1232
BsgI GTGCAG 1 cut(s) 1281
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 164, 318, 668
BshTI ACCGGT 1 cut(s) 406
BshVI ATCGAT 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 992, 1359
BsiHKCI CYCGRG 2 cut(s) 981, 1320
BsiSI CCGG 3 cut(s) 312, 407, 1249
BslFI GGGAC 3 cut(s) 780, 1049, 1197
BslI CCNNNNNNNGG 3 cut(s) 413, 581, 923
BsmAI GTCTC 2 cut(s) 36, 586
BsmFI GGGAC 3 cut(s) 780, 1049, 1197
BsnI GGCC 3 cut(s) 164, 318, 668
Bso31I GGTCTC 1 cut(s) 36
BsoBI CYCGRG 2 cut(s) 981, 1320
Bsp1286I GDGCHC 2 cut(s) 992, 1359
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 5 cut(s) 398, 640, 716, 1047, 1285
Bsp19I CCATGG 1 cut(s) 139
BspACI CCGC 6 cut(s) 180, 226, 315, 521, 658, 877
BspANI GGCC 3 cut(s) 164, 318, 668
BspCNI CTCAG 3 cut(s) 1173, 1321, 1371
BspDI ATCGAT 1 cut(s) 1050
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 1272
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 2 cut(s) 724, 1293
BspQI GCTCTTC 1 cut(s) 564
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 1156
BsrFI RCCGGY 2 cut(s) 406, 1248
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 4 cut(s) 634, 824, 928, 1287
BssAI RCCGGY 2 cut(s) 406, 1248
BssECI CCNNGG 3 cut(s) 139, 327, 1266
BssMI GATC 5 cut(s) 398, 640, 716, 1047, 1285
BssNI GRCGYC 2 cut(s) 624, 1084
BssT1I CCWWGG 1 cut(s) 139
Bst2UI CCWGG 4 cut(s) 328, 576, 918, 1268
Bst4CI ACNGT 7 cut(s) 362, 534, 760, 798, 898, 960, 1352
Bst6I CTCTTC 6 cut(s) 242, 483, 564, 628, 843, 1085
BstACI GRCGYC 2 cut(s) 624, 1084
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 4 cut(s) 7, 739, 1234, 1382
BstDEI CTNAG 4 cut(s) 1160, 1178, 1308, 1358
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 461, 666
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 4 cut(s) 215, 230, 370, 1188
BstKTI GATC 5 cut(s) 401, 643, 719, 1050, 1288
BstMAI GTCTC 2 cut(s) 36, 586
BstMBI GATC 5 cut(s) 398, 640, 716, 1047, 1285
BstMWI GCNNNNNNNGC 7 cut(s) 11, 156, 219, 868, 1146, 1183, 1242
BstNI CCWGG 4 cut(s) 328, 576, 918, 1268
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 4 cut(s) 326, 574, 916, 1266
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 992
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 1232
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
Bsu15I ATCGAT 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 3 cut(s) 164, 318, 668
BsuTUI ATCGAT 1 cut(s) 1050
BtgI CCRYGG 1 cut(s) 139
BtgZI GCGATG 1 cut(s) 594
BtsCI GGATG 2 cut(s) 461, 666
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 7 cut(s) 16, 285, 627, 756, 921, 956, 1357
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 4 cut(s) 7, 739, 1234, 1382
CaiI CAGNNNCTG 1 cut(s) 758
CfoI GCGC 4 cut(s) 215, 230, 370, 1188
Cfr10I RCCGGY 2 cut(s) 406, 1248
Cfr13I GGNCC 2 cut(s) 317, 1270
ClaI ATCGAT 1 cut(s) 1050
CseI GACGC 2 cut(s) 632, 1073
Csp6I GTAC 1 cut(s) 347
CspAI ACCGGT 1 cut(s) 406
CviAII CATG 7 cut(s) 140, 274, 350, 720, 743, 868, 1331
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 4 cut(s) 1160, 1178, 1308, 1358
DpnI GATC 5 cut(s) 400, 642, 718, 1049, 1287
DpnII GATC 5 cut(s) 398, 640, 716, 1047, 1285
DrdI GACNNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 1061
Eam1104I CTCTTC 6 cut(s) 242, 483, 564, 628, 843, 1085
EarI CTCTTC 6 cut(s) 242, 483, 564, 628, 843, 1085
EciI GGCGGA 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1270
Eco57I CTGAAG 3 cut(s) 588, 617, 1110
Eco88I CYCGRG 2 cut(s) 981, 1320
EcoO109I RGGNCCY 1 cut(s) 1270
EcoRI GAATTC 1 cut(s) 1038
EcoRII CCWGG 4 cut(s) 326, 574, 916, 1266
EcoT14I CCWWGG 1 cut(s) 139
EcoT22I ATGCAT 1 cut(s) 725
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 7 cut(s) 143, 277, 353, 723, 746, 871, 1334
FaqI GGGAC 3 cut(s) 780, 1049, 1197
FatI CATG 7 cut(s) 139, 273, 349, 719, 742, 867, 1330
FauI CCCGC 1 cut(s) 528
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 316, 877, 1246
FokI GGATG 2 cut(s) 448, 673
Fsp4HI GCNGC 3 cut(s) 316, 877, 1246
FspBI CTAG 4 cut(s) 387, 444, 515, 608
FspI TGCGCA 1 cut(s) 1187
GlaI GCGC 4 cut(s) 214, 229, 369, 1187
GluI GCNGC 3 cut(s) 316, 877, 1246
HaeIII GGCC 3 cut(s) 164, 318, 668
HapII CCGG 3 cut(s) 312, 407, 1249
HgaI GACGC 2 cut(s) 632, 1073
HhaI GCGC 4 cut(s) 215, 230, 370, 1188
Hin1I GRCGYC 2 cut(s) 624, 1084
Hin1II CATG 7 cut(s) 143, 277, 353, 723, 746, 871, 1334
Hin6I GCGC 4 cut(s) 213, 228, 368, 1186
HinP1I GCGC 4 cut(s) 213, 228, 368, 1186
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 5 cut(s) 200, 207, 806, 1055, 1130
HpaII CCGG 3 cut(s) 312, 407, 1249
HphI GGTGA 2 cut(s) 103, 253
Hpy166II GTNNAC 2 cut(s) 562, 990
Hpy188I TCNGA 6 cut(s) 568, 1089, 1129, 1171, 1257, 1390
Hpy188III TCNNGA 6 cut(s) 204, 842, 983, 1043, 1193, 1214
Hpy8I GTNNAC 2 cut(s) 562, 990
Hpy99I CGWCG 2 cut(s) 200, 305
HpyAV CCTTC 6 cut(s) 316, 330, 757, 987, 1074, 1284
HpyCH4III ACNGT 7 cut(s) 362, 534, 760, 798, 898, 960, 1352
HpyCH4IV ACGT 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 156, 219, 868, 1146, 1183, 1242
HpyF3I CTNAG 4 cut(s) 1160, 1178, 1308, 1358
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 2 cut(s) 624, 1084
Hsp92II CATG 7 cut(s) 143, 277, 353, 723, 746, 871, 1334
HspAI GCGC 4 cut(s) 213, 228, 368, 1186
Kzo9I GATC 5 cut(s) 398, 640, 716, 1047, 1285
LguI GCTCTTC 1 cut(s) 564
Lsp1109I GCAGC 1 cut(s) 1232
LweI GCATC 4 cut(s) 58, 345, 373, 601
MaeI CTAG 4 cut(s) 387, 444, 515, 608
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 5 cut(s) 259, 402, 603, 616, 954
MalI GATC 5 cut(s) 400, 642, 718, 1049, 1287
MboI GATC 5 cut(s) 398, 640, 716, 1047, 1285
MfeI CAATTG 3 cut(s) 93, 483, 993
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 992, 1359
MluCI AATT 8 cut(s) 88, 93, 376, 483, 993, 1038, 1289, 1345
MlyI GAGTC 2 cut(s) 1049, 1139
MmeI TCCRAC 2 cut(s) 291, 400
Mph1103I ATGCAT 1 cut(s) 725
MseI TTAA 2 cut(s) 930, 1104
MslI CAYNNNNRTG 2 cut(s) 81, 995
MspA1I CMGCKG 1 cut(s) 1007
MspI CCGG 3 cut(s) 312, 407, 1249
MspR9I CCNGG 4 cut(s) 328, 576, 918, 1268
MunI CAATTG 3 cut(s) 93, 483, 993
MvaI CCWGG 4 cut(s) 328, 576, 918, 1268
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 7 cut(s) 11, 156, 219, 868, 1146, 1183, 1242
NcoI CCATGG 1 cut(s) 139
NdeII GATC 5 cut(s) 398, 640, 716, 1047, 1285
NlaIII CATG 7 cut(s) 143, 277, 353, 723, 746, 871, 1334
NlaIV GGNNCC 1 cut(s) 1272
NmuCI GTSAC 2 cut(s) 259, 954
NsbI TGCGCA 1 cut(s) 1187
NsiI ATGCAT 1 cut(s) 725
NspI RCATGY 1 cut(s) 871
OliI CACNNNNGTG 1 cut(s) 995
PciSI GCTCTTC 1 cut(s) 564
PfeI GAWTC 3 cut(s) 200, 207, 806
PinAI ACCGGT 1 cut(s) 406
PkrI GCNGC 3 cut(s) 317, 878, 1247
PleI GAGTC 2 cut(s) 1049, 1138
PpsI GAGTC 2 cut(s) 1049, 1138
PpuMI RGGWCCY 1 cut(s) 1270
PshBI ATTAAT 1 cut(s) 930
Psp5II RGGWCCY 1 cut(s) 1270
Psp6I CCWGG 4 cut(s) 326, 574, 916, 1266
PspGI CCWGG 4 cut(s) 326, 574, 916, 1266
PspN4I GGNNCC 1 cut(s) 1272
PspPI GGNCC 2 cut(s) 317, 1270
PspPPI RGGWCCY 1 cut(s) 1270
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 2 cut(s) 81, 995
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 2 cut(s) 930, 1104
SatI GCNGC 3 cut(s) 316, 877, 1246
Sau3AI GATC 5 cut(s) 398, 640, 716, 1047, 1285
Sau96I GGNCC 2 cut(s) 317, 1270
SchI GAGTC 2 cut(s) 1049, 1139
ScrFI CCNGG 4 cut(s) 328, 576, 918, 1268
SduI GDGCHC 2 cut(s) 992, 1359
SfaNI GCATC 4 cut(s) 58, 345, 373, 601
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 1 cut(s) 1270
SmiMI CAYNNNNRTG 2 cut(s) 81, 995
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 8 cut(s) 88, 93, 376, 483, 993, 1038, 1289, 1345
SsiI CCGC 6 cut(s) 180, 226, 315, 521, 658, 877
SspMI CTAG 4 cut(s) 387, 444, 515, 608
StyD4I CCNGG 4 cut(s) 326, 574, 916, 1266
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 7 cut(s) 362, 534, 760, 798, 898, 960, 1352
TaiI ACGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 728, 1050, 1078, 1241
TasI AATT 8 cut(s) 88, 93, 376, 483, 993, 1038, 1289, 1345
TatI WGTACW 1 cut(s) 346
TauI GCSGC 2 cut(s) 318, 879
TfiI GAWTC 3 cut(s) 200, 207, 806
Tru1I TTAA 2 cut(s) 930, 1104
Tru9I TTAA 2 cut(s) 930, 1104
TscAI CASTG 7 cut(s) 16, 292, 634, 763, 928, 963, 1357
TseFI GTSAC 2 cut(s) 259, 954
TseI GCWGC 1 cut(s) 1245
Tsp45I GTSAC 2 cut(s) 259, 954
TspDTI ATGAA 7 cut(s) 453, 483, 933, 1016, 1128, 1132, 1266
TspRI CASTG 7 cut(s) 16, 292, 634, 763, 928, 963, 1357
VneI GTGCAC 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 1270
VspI ATTAAT 1 cut(s) 930
XapI RAATTY 2 cut(s) 376, 1038
XceI RCATGY 1 cut(s) 871
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 4 cut(s) 387, 444, 515, 608
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.