Rw3G022300

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013576
Physical Location & Seq
Forward (+)
27297304 .. 27300535
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022300.1

Sequence Viewer

Length: 1140 bp
ATGATTTCTCCCACTGATAACGTCCTCGTCGCCTTCTCCGGCGGCCCTTCTTCCAGGGTTGCTTTACAGTTTGTACATGAGATGCACAGTAAAGCACAGAAGAATTTTGATGCTAGTAGGGATAGATCGTTACCGGTGTTCGGTGTCGGAGTTGCATTTATTGATGAAACTAGTGTTTATTCTGTTCCATCCAATGAACAAGACAATGCAATTGCAGACATTACATCTATTGTGGAAAACCTAGCCCCGCCGATAAAACAATTGTACGTTGTACCGATTGAGAGTGTCTACTCTGAAGAGCCTGGCGATGGCAGGGAGAGACTGAAGAAGTTACTAGATGCTGTTACTGACGCCACTGGAAGAGAAGATCTTTTACTACATCTGCGGATGTTGGCCTTGCAAAAGATTGCCTCACAAAATGGATACAACAGACTTCTACTGGGATCATGCATATCCAGGATTGCTTGCCATGTTATTACAGCCACTGTGAAGGGACAAGGATATTCACTACCAGCAGATATACAGTATGTTGATTCAAGGTGGGAAATCCCAGTTTTGCTTCCTCTTCATGATTGTCTTGCACAAGAGCTGAACATGCTTTGCTGCCTTGATGGTCTAAAAACTGTTGAGTTGATAAGAAGTCCTGGCACTGGCATTAATGGGTTGGTTTCATCATTTGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGGGAGTGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCGAGAGATCGATGACTCTAATGTCCCTTTGGCAACTCGAAGGCGTCAGAAGAGATATAACCTTAAACCTAATGAATGTTTTTCTTCAGAGTCGTTCTGCTTCATCTGCAATGGCCCACTCAGCAAATCTGATTTGCTAAGTTTGCGCAATCTTGAACAATGCCAAACAAGTCCCAACACTTTGGGTGGTGTCTGCTGTTCGAGCTGCTGGTTTCAGATAATTCCCCAGGACCCTTCATTACTGGATCAATTTTCTGCAGTTTTACCTCAGCAGCTGGTCGCTCGGGCAAAGCATGACAGCCTTGACAATTACAGTGCGCTCAGGGAACAAATACAAGATTGCTTGCTATCAGAAGGCGAAGACGAAACCTGA

Protein Analysis

379

Amino Acids

41.94

Weight (kDa)

5.2

Isoelectric Point (pI)

57.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
Acc16I TGCGCA 1 cut(s) 914
AccI GTMKAC 1 cut(s) 288
AciI CCGC 3 cut(s) 42, 248, 385
AclWI GGATC 2 cut(s) 451, 1020
AcsI RAATTY 2 cut(s) 103, 765
AcuI CTGAAG 3 cut(s) 315, 344, 837
AcyI GRCGYC 2 cut(s) 351, 811
AfaI GTAC 3 cut(s) 75, 266, 273
AfiI CCNNNNNNNGG 3 cut(s) 140, 308, 650
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 3 cut(s) 537, 760, 923
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 5 cut(s) 53, 301, 455, 643, 993
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 5 cut(s) 589, 734, 742, 972, 1042
AluI AGCT 5 cut(s) 589, 734, 742, 972, 1042
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 1 cut(s) 313
Alw44I GTGCAC 1 cut(s) 715
AlwI GGATC 2 cut(s) 451, 1020
AlwNI CAGNNNCTG 2 cut(s) 485, 1042
Ama87I CYCGRG 2 cut(s) 708, 1050
AoxI GGCC 3 cut(s) 43, 393, 880
ApaLI GTGCAC 1 cut(s) 715
ApeKI GCWGC 3 cut(s) 603, 972, 1039
ApoI RAATTY 2 cut(s) 103, 765
AseI ATTAAT 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 2 cut(s) 915, 1087
AspS9I GGNCC 3 cut(s) 44, 881, 997
AvaI CYCGRG 2 cut(s) 708, 1050
AvaII GGWCC 1 cut(s) 997
BaeGI GKGCMC 1 cut(s) 719
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 1134
Bbv12I GWGCWC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 1035
BbvI GCAGC 3 cut(s) 590, 959, 1051
BccI CCATC 3 cut(s) 196, 302, 605
BciT130I CCWGG 5 cut(s) 55, 303, 457, 645, 995
BciVI GTATCC 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 313
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 4 cut(s) 114, 171, 242, 335
BfmI CTRYAG 1 cut(s) 1023
BfuI GTATCC 1 cut(s) 416
BglII AGATCT 1 cut(s) 367
BisI GCNGC 4 cut(s) 43, 604, 973, 1040
BlsI GCNGC 4 cut(s) 44, 605, 974, 1041
Bme1390I CCNGG 5 cut(s) 55, 303, 457, 645, 995
Bme18I GGWCC 1 cut(s) 997
BmeT110I CYCGRG 2 cut(s) 708, 1050
BmgT120I GGNCC 3 cut(s) 44, 881, 997
BmiI GGNNCC 1 cut(s) 999
BmrFI CCNGG 5 cut(s) 55, 303, 457, 645, 995
BmrI ACTGGG 2 cut(s) 449, 545
BmsI GCATC 3 cut(s) 72, 100, 328
BmuI ACTGGG 2 cut(s) 449, 545
BpiI GAAGAC 1 cut(s) 1134
Bpu10I CCTNAGC 2 cut(s) 1035, 1088
Bsa29I ATCGAT 1 cut(s) 777
BsaHI GRCGYC 2 cut(s) 351, 811
BsaJI CCNNGG 2 cut(s) 54, 993
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 308, 650
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 5 cut(s) 361, 444, 551, 655, 1014
Bse3DI GCAATG 1 cut(s) 883
BseBI CCWGG 5 cut(s) 55, 303, 457, 645, 995
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 54, 993
BseGI GGATG 2 cut(s) 188, 393
BseLI CCNNNNNNNGG 3 cut(s) 140, 308, 650
BseMI GCAATG 1 cut(s) 883
BseMII CTCAG 3 cut(s) 901, 1049, 1102
BseNI ACTGG 5 cut(s) 361, 444, 551, 655, 1014
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 3 cut(s) 590, 959, 1051
BshFI GGCC 3 cut(s) 45, 395, 882
BshTI ACCGGT 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 719
BsiHKCI CYCGRG 2 cut(s) 708, 1050
BsiSI CCGG 2 cut(s) 39, 134
BslFI GGGAC 3 cut(s) 507, 776, 924
BslI CCNNNNNNNGG 3 cut(s) 140, 308, 650
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 3 cut(s) 507, 776, 924
BsnI GGCC 3 cut(s) 45, 395, 882
BsoBI CYCGRG 2 cut(s) 708, 1050
Bsp1286I GDGCHC 1 cut(s) 719
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 5 cut(s) 125, 367, 443, 774, 1012
BspACI CCGC 3 cut(s) 42, 248, 385
BspANI GGCC 3 cut(s) 45, 395, 882
BspCNI CTCAG 3 cut(s) 900, 1048, 1101
BspDI ATCGAT 1 cut(s) 777
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 999
BspMAI CTGCAG 1 cut(s) 1027
BspPI GGATC 2 cut(s) 451, 1020
BspQI GCTCTTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 883
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 73
BsrI ACTGG 5 cut(s) 361, 444, 551, 655, 1014
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 54, 993
BssMI GATC 5 cut(s) 125, 367, 443, 774, 1012
BssNI GRCGYC 2 cut(s) 351, 811
Bst2UI CCWGG 5 cut(s) 55, 303, 457, 645, 995
Bst4CI ACNGT 6 cut(s) 69, 89, 487, 525, 625, 1082
Bst6I CTCTTC 4 cut(s) 291, 355, 570, 812
BstACI GRCGYC 2 cut(s) 351, 811
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 466, 1112
BstDEI CTNAG 4 cut(s) 887, 905, 1035, 1088
BstF5I GGATG 2 cut(s) 188, 393
BstHHI GCGC 2 cut(s) 915, 1087
BstKTI GATC 5 cut(s) 128, 370, 446, 777, 1015
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 5 cut(s) 125, 367, 443, 774, 1012
BstMWI GCNNNNNNNGC 5 cut(s) 595, 873, 888, 910, 969
BstNI CCWGG 5 cut(s) 55, 303, 457, 645, 995
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 5 cut(s) 53, 301, 455, 643, 993
BstSFI CTRYAG 1 cut(s) 1023
BstSLI GKGCMC 1 cut(s) 719
BstV1I GCAGC 3 cut(s) 590, 959, 1051
BstV2I GAAGAC 1 cut(s) 1134
BstX2I RGATCY 1 cut(s) 367
BstXI CCANNNNNNTGG 1 cut(s) 949
BstYI RGATCY 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 777
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 3 cut(s) 45, 395, 882
BsuTUI ATCGAT 1 cut(s) 777
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 2 cut(s) 188, 393
BtsIMutI CAGTG 5 cut(s) 12, 354, 483, 648, 1087
Cac8I GCNNGC 2 cut(s) 466, 1112
CaiI CAGNNNCTG 2 cut(s) 485, 1042
CciI TCATGA 1 cut(s) 568
CfoI GCGC 2 cut(s) 915, 1087
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 44, 881, 997
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 2 cut(s) 359, 800
Csp6I GTAC 3 cut(s) 74, 265, 272
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 6 cut(s) 77, 447, 470, 569, 595, 1061
CviQI GTAC 3 cut(s) 74, 265, 272
DdeI CTNAG 4 cut(s) 887, 905, 1035, 1088
DpnI GATC 5 cut(s) 127, 369, 445, 776, 1014
DpnII GATC 5 cut(s) 125, 367, 443, 774, 1012
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
Eam1104I CTCTTC 4 cut(s) 291, 355, 570, 812
EarI CTCTTC 4 cut(s) 291, 355, 570, 812
Eco47I GGWCC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 315, 344, 837
Eco88I CYCGRG 2 cut(s) 708, 1050
EcoO109I RGGNCCY 1 cut(s) 997
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 5 cut(s) 53, 301, 455, 643, 993
EcoT22I ATGCAT 1 cut(s) 452
FaeI CATG 6 cut(s) 80, 450, 473, 572, 598, 1064
FaqI GGGAC 3 cut(s) 507, 776, 924
FatI CATG 6 cut(s) 76, 446, 469, 568, 594, 1060
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 4 cut(s) 43, 604, 973, 1040
FokI GGATG 2 cut(s) 175, 400
Fsp4HI GCNGC 4 cut(s) 43, 604, 973, 1040
FspBI CTAG 4 cut(s) 114, 171, 242, 335
FspI TGCGCA 1 cut(s) 914
GlaI GCGC 2 cut(s) 914, 1086
GluI GCNGC 4 cut(s) 43, 604, 973, 1040
HaeIII GGCC 3 cut(s) 45, 395, 882
HapII CCGG 2 cut(s) 39, 134
HgaI GACGC 2 cut(s) 359, 800
HhaI GCGC 2 cut(s) 915, 1087
Hin1I GRCGYC 2 cut(s) 351, 811
Hin1II CATG 6 cut(s) 80, 450, 473, 572, 598, 1064
Hin6I GCGC 2 cut(s) 913, 1085
HinP1I GCGC 2 cut(s) 913, 1085
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 3 cut(s) 533, 782, 857
HpaII CCGG 2 cut(s) 39, 134
Hpy166II GTNNAC 2 cut(s) 289, 717
Hpy188I TCNGA 7 cut(s) 149, 295, 816, 856, 898, 984, 1120
Hpy188III TCNNGA 4 cut(s) 569, 710, 770, 920
Hpy8I GTNNAC 2 cut(s) 289, 717
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 7 cut(s) 43, 57, 484, 714, 801, 1011, 1115
HpyCH4III ACNGT 6 cut(s) 69, 89, 487, 525, 625, 1082
HpyCH4IV ACGT 2 cut(s) 21, 267
HpyF10VI GCNNNNNNNGC 5 cut(s) 595, 873, 888, 910, 969
HpyF3I CTNAG 4 cut(s) 887, 905, 1035, 1088
HpySE526I ACGT 2 cut(s) 21, 267
Hsp92I GRCGYC 2 cut(s) 351, 811
Hsp92II CATG 6 cut(s) 80, 450, 473, 572, 598, 1064
HspAI GCGC 2 cut(s) 913, 1085
Kzo9I GATC 5 cut(s) 125, 367, 443, 774, 1012
LguI GCTCTTC 1 cut(s) 291
Lsp1109I GCAGC 3 cut(s) 590, 959, 1051
LweI GCATC 3 cut(s) 72, 100, 328
MaeI CTAG 4 cut(s) 114, 171, 242, 335
MaeII ACGT 2 cut(s) 21, 267
MaeIII GTNAC 4 cut(s) 129, 330, 343, 681
MalI GATC 5 cut(s) 127, 369, 445, 776, 1014
MboI GATC 5 cut(s) 125, 367, 443, 774, 1012
MfeI CAATTG 3 cut(s) 210, 260, 720
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 8 cut(s) 103, 210, 260, 720, 765, 987, 1016, 1075
MlyI GAGTC 2 cut(s) 776, 866
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 4 cut(s) 35, 421, 573, 1044
Mph1103I ATGCAT 1 cut(s) 452
MseI TTAA 2 cut(s) 657, 831
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 734, 1042
MspI CCGG 2 cut(s) 39, 134
MspR9I CCNGG 5 cut(s) 55, 303, 457, 645, 995
MunI CAATTG 3 cut(s) 210, 260, 720
MvaI CCWGG 5 cut(s) 55, 303, 457, 645, 995
MwoI GCNNNNNNNGC 5 cut(s) 595, 873, 888, 910, 969
NdeII GATC 5 cut(s) 125, 367, 443, 774, 1012
NlaIII CATG 6 cut(s) 80, 450, 473, 572, 598, 1064
NlaIV GGNNCC 1 cut(s) 999
NmuCI GTSAC 1 cut(s) 681
NsbI TGCGCA 1 cut(s) 914
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 1 cut(s) 598
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 455
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 4 cut(s) 44, 605, 974, 1041
PleI GAGTC 2 cut(s) 776, 865
PpsI GAGTC 2 cut(s) 776, 865
PpuMI RGGWCCY 1 cut(s) 997
PshBI ATTAAT 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 5 cut(s) 53, 301, 455, 643, 993
PspGI CCWGG 5 cut(s) 53, 301, 455, 643, 993
PspN4I GGNNCC 1 cut(s) 999
PspPI GGNCC 3 cut(s) 44, 881, 997
PspPPI RGGWCCY 1 cut(s) 997
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 2 cut(s) 485, 1042
PsuI RGATCY 1 cut(s) 367
PvuII CAGCTG 2 cut(s) 734, 1042
RsaI GTAC 3 cut(s) 75, 266, 273
RsaNI GTAC 3 cut(s) 74, 265, 272
RseI CAYNNNNRTG 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 657, 831
SatI GCNGC 4 cut(s) 43, 604, 973, 1040
Sau3AI GATC 5 cut(s) 125, 367, 443, 774, 1012
Sau96I GGNCC 3 cut(s) 44, 881, 997
SchI GAGTC 2 cut(s) 776, 866
ScrFI CCNGG 5 cut(s) 55, 303, 457, 645, 995
SduI GDGCHC 1 cut(s) 719
SfaNI GCATC 3 cut(s) 72, 100, 328
SfcI CTRYAG 1 cut(s) 1023
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 170
Sse9I AATT 8 cut(s) 103, 210, 260, 720, 765, 987, 1016, 1075
SsiI CCGC 3 cut(s) 42, 248, 385
SspMI CTAG 4 cut(s) 114, 171, 242, 335
StyD4I CCNGG 5 cut(s) 53, 301, 455, 643, 993
TaaI ACNGT 6 cut(s) 69, 89, 487, 525, 625, 1082
TaiI ACGT 2 cut(s) 24, 270
TaqI TCGA 4 cut(s) 769, 777, 805, 968
TasI AATT 8 cut(s) 103, 210, 260, 720, 765, 987, 1016, 1075
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 2 cut(s) 657, 831
Tru9I TTAA 2 cut(s) 657, 831
TscAI CASTG 5 cut(s) 19, 361, 490, 655, 1087
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 3 cut(s) 603, 972, 1039
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 8 cut(s) 180, 210, 557, 660, 743, 855, 859, 993
TspRI CASTG 5 cut(s) 19, 361, 490, 655, 1087
VneI GTGCAC 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 997
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 2 cut(s) 103, 765
XceI RCATGY 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 4 cut(s) 114, 171, 242, 335
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.