RLG00000023353

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013576
Physical Location & Seq
Reverse (-)
22390093 .. 22394651
4559 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023353

Sequence Viewer

Length: 2145 bp
ATGCCCCAAAAGGGTGTATTGTTCATGTACAATTCTCTCATAAGGGGTTACTCTAGTGCTGGGCTTTGTGATGACCCTATTGGGCTCTATGTTCAGATGGTGCTTCCGGGTGTTTTGCCGGATAAGTTTACATTCCCGTTTGCATTGAGCGCGTGCTCAAAGATTGTGGCTTTCTGTGAAGGGGTTCAGCTCCATGGCTCGCTTGTGAAGATGGGTTTCGAGCAAGATGTGTTTATTGGGAATCCTTTGATTCAATTCTATGCAGAATGTGGGGACTTGGATTATGCCCACAAGGTGTTTGATGAAATGCTTGAGAAAAACATCGTGTCGTGGACTAGTTTGATTTGTGGTTATGGTAGAAGGAATATGCCCAAGGAGGCTGTTTCTTTGTTTTTGGAGATGGTGGCTGCCGGTGTTAAGCCCAATTCGGTGACTATGGTGTGTGTCATTTCTGCTTGTGCAAAGTTGAAGGATGTTGCGTTGAGTGAGAGAGTGTGTGCTTACATTGGGGAGTCTGGACTGAAGACTAATATGCTTCTGGTGAATTCACTTGTTGATATGTATATGAAATGTGGAGCTACTGATGCCACAAAGCGGCTTTTTGATGAATGTGTCGATAAAAACTTGGTTCTTTACAATAACATTTTGTCAAATTATGTGCGCCAGGGACTGGCTAGTGAAGTAGTTTCTGTCATGGGTCAAATGCTACAACAGGGCCTAAGATCTGACAAGGTTACCATGTTAACTGCAATTTCAGCTTGCGCACAACCAAGTGATTCTCTCTCAGAAGTAGAGAAAGAAGATGCCAAACACATCAATACCAATTCAATTGCTACCAACACAATCAGCAGCAACAACAGAAACGACAGCCATGGCTTGTGCCTCAAGTGCAAGGCCAACCCTCCCATCTCCGCCGATACCCACGCCGACGATTCCCGTTTCTGCGCCGATTGCTTCCGCGCAAACCTCTTCGGCAAGTTCAGGTTCGCCGTCACCTCCAACGCCATGATTTCTCCCACTGATAACGTCCTCGTCGCCTTCTCTGGCGGCCCTTCTTCCAGGGTTGCTTTACAGTTTGTACATGAGATGCACAGTAAAGCGCAGAAAAATTTTCATGCTAGTAGGGATAGATCGTTACCGGTGTTCGGTGTTGGAGTTGCATTTATTGATGAAACTAGTGTTTATTCTGTTCCATCCAATGAACAAGACAATGCAATTGCAGACATTACATCTATTGTGGAAAGCCTAGCCCCGCAGACAAAACAATTGTACGTTGTACCGATTGAGAGTGTCTACTCTGAAGAGTCTGGCGATGGCAGGGAGAGACTAAAGAAGTTACTAGATGCTGTTACTGACGCCACCGGAAGAGAAGATCTTTTACTACATCTGCGGATGTTGGCCTTGCAAAAGATTGCCTCACAAAATGGATACAGCAGACTTCTACTGGGATCATGCGTATCGAGGATTGCTTGCCATGTTATTACAGCCACTGTGAAGGGACAAGGATATTCACTACCAGCAGATATACAGTATGTTGATTCAAGGTGGGAAATCCCAGTTTTGCTTCCTCTTCGTGATTGTCTTGCACAAGAGCTGAACATGCTTTGCCGCCTTGATGGTCTAAAAACTGTTGAGTTGATAAGAAGTCCTGGCTCTGGCATTAATGGGTTGGTTTCATCATTTGTGTCACTCTTGCAGGAAGAAAATCCTTCTCGGGAGTGCACAATTGTGAGAACAGCTGGAAAGCTTATCCCATTTCATTTCAACAGAATTCAAGAGATTGATGACTCTAATGTCCCTTTGGCAACTCGAAGGCGTCAGAAGAGATATAACCTTAAACCTAATGAATGTTTTTCTTCAGAGTCATTCTGCATTATCTGCAATGGCCCACTCAACAAATCTGATTTGCTAAGTTTGCGCAATCTTGAACAATGCCAAACATGTCCCGACACTTTTGGTGGTGCCTGCTGTTCGAGCTGCCGGTTTCAGATACTTCCCCAGGACCCTTCATTACTGGATCAATTTTCTGCTGTTTTACCCCAGCAGCTGGTTGCTCGGGCAAAGCATGACAACCTTAACAATTACAGTGTGCTCAGGGAACAAATGCAAGATTGCTTGCTTTCAGAAGGCGAAGATGAAACCTGA

Protein Analysis

715

Amino Acids

78.59

Weight (kDa)

5.68

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 106 - 155 5.1e-13 PPR repeat family
PPR PF01535 109 - 139 9.4e-08 PPR repeat
PPR_2 PF13041 208 - 255 7.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013576)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35910
fragaria_vesca FvH4_6g23820
malus_domestica MD12G1074000.v1.1
prunus_persica Prupe.7G060900_v2.0.a1
pyrus_communis pycom12g06390
rosa_chinensis RchiOBHm_Chr3g0482381
rosa_laevigata RLG00000023353 RLG00000023355 RLG00000023356
rosa_multiflora Rmu_sc0008293.1_g000002 Rmu_sc0013521.1_g000004
rosa_roxburghii Rroxscaffold_6G00399370
rosa_rugosa Rorug03G0195900
rosa_samantha Rh3AG247900 Rh3BG282400 Rh3CG279400
rosa_wichuraiana Rw3G022220 Rw3G022300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1793
Acc16I TGCGCA 2 cut(s) 763, 1919
AccB1I GGYRCC 1 cut(s) 1961
AccB7I CCANNNNNTGG 2 cut(s) 670, 2047
AccI GTMKAC 1 cut(s) 1293
AccII CGCG 2 cut(s) 152, 960
AciI CCGC 7 cut(s) 595, 912, 958, 1047, 1253, 1390, 1609
AclWI GGATC 2 cut(s) 1456, 2025
AcsI RAATTY 3 cut(s) 544, 1108, 1770
AcuI CTGAAG 3 cut(s) 542, 1320, 1842
AcyI GRCGYC 2 cut(s) 1356, 1816
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 4 cut(s) 29, 1080, 1271, 1278
AfiI CCNNNNNNNGG 6 cut(s) 11, 594, 670, 1145, 1655, 2047
AflIII ACRYGT 1 cut(s) 1940
AgeI ACCGGT 1 cut(s) 1138
AgsI TTSAA 7 cut(s) 254, 469, 828, 1542, 1765, 1775, 1928
AhlI ACTAGT 2 cut(s) 335, 1175
AjnI CCWGG 4 cut(s) 663, 1058, 1648, 1998
AleI CACNNNNGTG 1 cut(s) 1727
AluBI AGCT 8 cut(s) 190, 578, 758, 1594, 1739, 1747, 1977, 2047
AluI AGCT 8 cut(s) 190, 578, 758, 1594, 1739, 1747, 1977, 2047
Alw21I GWGCWC 3 cut(s) 158, 1724, 2094
Alw26I GTCTC 1 cut(s) 1318
Alw44I GTGCAC 1 cut(s) 1720
AlwI GGATC 2 cut(s) 1456, 2025
AlwNI CAGNNNCTG 3 cut(s) 670, 1490, 2047
Ama87I CYCGRG 2 cut(s) 1713, 2055
AoxI GGCC 5 cut(s) 715, 894, 1048, 1398, 1885
ApaLI GTGCAC 1 cut(s) 1720
ApeKI GCWGC 4 cut(s) 407, 849, 1977, 2044
ApoI RAATTY 3 cut(s) 544, 1108, 1770
AseI ATTAAT 1 cut(s) 1662
AsiGI ACCGGT 1 cut(s) 1138
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 7 cut(s) 152, 663, 764, 947, 962, 1102, 1920
AspS9I GGNCC 4 cut(s) 715, 1049, 1886, 2002
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 442, 553, 985
AvaI CYCGRG 2 cut(s) 1713, 2055
AvaII GGWCC 1 cut(s) 2002
BaeGI GKGCMC 1 cut(s) 1724
BanI GGYRCC 1 cut(s) 1961
BanII GRGCYC 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 1362, 1394
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 3 cut(s) 158, 1724, 2094
BbvI GCAGC 4 cut(s) 394, 861, 1964, 2056
BccI CCATC 7 cut(s) 91, 205, 394, 914, 1201, 1307, 1610
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 4 cut(s) 665, 1060, 1650, 2000
BciVI GTATCC 1 cut(s) 1421
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 1318
BcuI ACTAGT 2 cut(s) 335, 1175
BfaI CTAG 7 cut(s) 54, 336, 675, 1119, 1176, 1247, 1340
BfuI GTATCC 1 cut(s) 1421
BglII AGATCT 2 cut(s) 722, 1372
BisI GCNGC 7 cut(s) 408, 596, 850, 1048, 1609, 1978, 2045
BlsI GCNGC 7 cut(s) 409, 597, 851, 1049, 1610, 1979, 2046
Bme1390I CCNGG 5 cut(s) 108, 665, 1060, 1650, 2000
Bme18I GGWCC 1 cut(s) 2002
BmeT110I CYCGRG 2 cut(s) 1713, 2055
BmgT120I GGNCC 4 cut(s) 715, 1049, 1886, 2002
BmiI GGNNCC 2 cut(s) 1963, 2004
BmrFI CCNGG 5 cut(s) 108, 665, 1060, 1650, 2000
BmrI ACTGGG 2 cut(s) 1454, 1550
BmsI GCATC 4 cut(s) 574, 793, 1077, 1333
BmuI ACTGGG 2 cut(s) 1454, 1550
BpiI GAAGAC 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 2093
BpuEI CTTGAG 2 cut(s) 332, 869
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 1356, 1816
BsaJI CCNNGG 6 cut(s) 193, 372, 664, 871, 1059, 1998
BsaWI WCCGGW 2 cut(s) 1138, 1361
Bsc4I CCNNNNNNNGG 6 cut(s) 11, 594, 670, 1145, 1655, 2047
Bse118I RCCGGY 3 cut(s) 410, 1138, 1980
Bse1I ACTGG 4 cut(s) 675, 1449, 1556, 2019
Bse3DI GCAATG 1 cut(s) 1888
BseBI CCWGG 4 cut(s) 665, 1060, 1650, 2000
BseDI CCNNGG 6 cut(s) 193, 372, 664, 871, 1059, 1998
BseGI GGATG 3 cut(s) 478, 1193, 1398
BseLI CCNNNNNNNGG 6 cut(s) 11, 594, 670, 1145, 1655, 2047
BseMI GCAATG 1 cut(s) 1888
BseMII CTCAG 2 cut(s) 798, 2107
BseNI ACTGG 4 cut(s) 675, 1449, 1556, 2019
BseSI GKGCMC 1 cut(s) 1724
BseXI GCAGC 4 cut(s) 394, 861, 1964, 2056
BseYI CCCAGC 2 cut(s) 59, 2040
Bsh1236I CGCG 2 cut(s) 152, 960
BshFI GGCC 5 cut(s) 717, 896, 1050, 1400, 1887
BshNI GGYRCC 1 cut(s) 1961
BshTI ACCGGT 1 cut(s) 1138
BsiHKAI GWGCWC 3 cut(s) 158, 1724, 2094
BsiHKCI CYCGRG 2 cut(s) 1713, 2055
BsiSI CCGG 6 cut(s) 107, 119, 411, 1139, 1362, 1981
BslFI GGGAC 5 cut(s) 287, 681, 1512, 1781, 1929
BslI CCNNNNNNNGG 6 cut(s) 11, 594, 670, 1145, 1655, 2047
BsmAI GTCTC 1 cut(s) 1318
BsmFI GGGAC 5 cut(s) 287, 681, 1512, 1781, 1929
BsnI GGCC 5 cut(s) 717, 896, 1050, 1400, 1887
BsoBI CYCGRG 2 cut(s) 1713, 2055
Bsp1286I GDGCHC 4 cut(s) 87, 158, 1724, 2094
Bsp1407I TGTACA 2 cut(s) 27, 1078
Bsp143I GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
Bsp19I CCATGG 2 cut(s) 193, 871
BspACI CCGC 7 cut(s) 595, 912, 958, 1047, 1253, 1390, 1609
BspANI GGCC 5 cut(s) 717, 896, 1050, 1400, 1887
BspCNI CTCAG 2 cut(s) 797, 2106
BspFNI CGCG 2 cut(s) 152, 960
BspLI GGNNCC 2 cut(s) 1963, 2004
BspPI GGATC 2 cut(s) 1456, 2025
BspT107I GGYRCC 1 cut(s) 1961
BsrDI GCAATG 1 cut(s) 1888
BsrFI RCCGGY 3 cut(s) 410, 1138, 1980
BsrGI TGTACA 2 cut(s) 27, 1078
BsrI ACTGG 4 cut(s) 675, 1449, 1556, 2019
BssAI RCCGGY 3 cut(s) 410, 1138, 1980
BssECI CCNNGG 6 cut(s) 193, 372, 664, 871, 1059, 1998
BssMI GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
BssNI GRCGYC 2 cut(s) 1356, 1816
BssT1I CCWWGG 3 cut(s) 193, 372, 871
Bst2UI CCWGG 4 cut(s) 665, 1060, 1650, 2000
Bst4CI ACNGT 6 cut(s) 1074, 1094, 1492, 1530, 1630, 2087
Bst6I CTCTTC 5 cut(s) 974, 1296, 1360, 1575, 1817
BstACI GRCGYC 2 cut(s) 1356, 1816
BstAPI GCANNNNNTGC 1 cut(s) 1878
BstAUI TGTACA 2 cut(s) 27, 1078
BstC8I GCNNGC 6 cut(s) 154, 200, 760, 1471, 1966, 2117
BstDEI CTNAG 4 cut(s) 719, 784, 1910, 2093
BstDSI CCRYGG 2 cut(s) 193, 871
BstEII GGTNACC 1 cut(s) 733
BstF5I GGATG 3 cut(s) 478, 1193, 1398
BstFNI CGCG 2 cut(s) 152, 960
BstHHI GCGC 7 cut(s) 152, 663, 764, 947, 962, 1102, 1920
BstKTI GATC 5 cut(s) 725, 1133, 1375, 1451, 2020
BstMAI GTCTC 1 cut(s) 1318
BstMBI GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
BstMWI GCNNNNNNNGC 9 cut(s) 149, 584, 755, 888, 951, 1600, 1878, 1915, 1974
BstNI CCWGG 4 cut(s) 665, 1060, 1650, 2000
BstNSI RCATGY 2 cut(s) 1603, 1944
BstPI GGTNACC 1 cut(s) 733
BstSCI CCNGG 5 cut(s) 106, 663, 1058, 1648, 1998
BstSLI GKGCMC 1 cut(s) 1724
BstUI CGCG 2 cut(s) 152, 960
BstV1I GCAGC 4 cut(s) 394, 861, 1964, 2056
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 722, 1372
BstYI RGATCY 2 cut(s) 722, 1372
BsuI GTATCC 1 cut(s) 1421
BsuRI GGCC 5 cut(s) 717, 896, 1050, 1400, 1887
BtgI CCRYGG 2 cut(s) 193, 871
BtgZI GCGATG 1 cut(s) 1326
BtsCI GGATG 3 cut(s) 478, 1193, 1398
BtsIMutI CAGTG 3 cut(s) 1017, 1488, 2092
Cac8I GCNNGC 6 cut(s) 154, 200, 760, 1471, 1966, 2117
CaiI CAGNNNCTG 3 cut(s) 670, 1490, 2047
CfoI GCGC 7 cut(s) 152, 663, 764, 947, 962, 1102, 1920
Cfr10I RCCGGY 3 cut(s) 410, 1138, 1980
Cfr13I GGNCC 4 cut(s) 715, 1049, 1886, 2002
CseI GACGC 2 cut(s) 1364, 1805
Csp6I GTAC 4 cut(s) 28, 1079, 1270, 1277
CspAI ACCGGT 1 cut(s) 1138
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviQI GTAC 4 cut(s) 28, 1079, 1270, 1277
DdeI CTNAG 4 cut(s) 719, 784, 1910, 2093
DpnI GATC 5 cut(s) 724, 1132, 1374, 1450, 2019
DpnII GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
DraIII CACNNNGTG 1 cut(s) 295
DrdI GACNNNNNNGTC 1 cut(s) 1793
DseDI GACNNNNNNGTC 1 cut(s) 1793
Eam1104I CTCTTC 5 cut(s) 974, 1296, 1360, 1575, 1817
EarI CTCTTC 5 cut(s) 974, 1296, 1360, 1575, 1817
EciI GGCGGA 1 cut(s) 901
Eco130I CCWWGG 3 cut(s) 193, 372, 871
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 2002
Eco57I CTGAAG 3 cut(s) 542, 1320, 1842
Eco88I CYCGRG 2 cut(s) 1713, 2055
Eco91I GGTNACC 1 cut(s) 733
EcoO109I RGGNCCY 2 cut(s) 715, 2002
EcoO65I GGTNACC 1 cut(s) 733
EcoRI GAATTC 2 cut(s) 544, 1770
EcoRII CCWGG 4 cut(s) 663, 1058, 1648, 1998
EcoT14I CCWWGG 3 cut(s) 193, 372, 871
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 193, 372, 871
FaqI GGGAC 5 cut(s) 287, 681, 1512, 1781, 1929
FauI CCCGC 1 cut(s) 1260
FblI GTMKAC 1 cut(s) 1293
Fnu4HI GCNGC 7 cut(s) 408, 596, 850, 1048, 1609, 1978, 2045
FokI GGATG 3 cut(s) 485, 1180, 1405
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 7 cut(s) 408, 596, 850, 1048, 1609, 1978, 2045
FspBI CTAG 7 cut(s) 54, 336, 675, 1119, 1176, 1247, 1340
FspI TGCGCA 2 cut(s) 763, 1919
GlaI GCGC 7 cut(s) 151, 662, 763, 946, 961, 1101, 1919
GluI GCNGC 7 cut(s) 408, 596, 850, 1048, 1609, 1978, 2045
GsaI CCCAGC 2 cut(s) 63, 2044
HaeIII GGCC 5 cut(s) 717, 896, 1050, 1400, 1887
HapII CCGG 6 cut(s) 107, 119, 411, 1139, 1362, 1981
HgaI GACGC 2 cut(s) 1364, 1805
HhaI GCGC 7 cut(s) 152, 663, 764, 947, 962, 1102, 1920
Hin1I GRCGYC 2 cut(s) 1356, 1816
Hin6I GCGC 7 cut(s) 150, 661, 762, 945, 960, 1100, 1918
HinP1I GCGC 7 cut(s) 150, 661, 762, 945, 960, 1100, 1918
HincII GTYRAC 1 cut(s) 744
HindII GTYRAC 1 cut(s) 744
HindIII AAGCTT 1 cut(s) 1745
HinfI GANTC 9 cut(s) 241, 250, 512, 776, 932, 1304, 1538, 1787, 1862
HpaI GTTAAC 1 cut(s) 744
HpaII CCGG 6 cut(s) 107, 119, 411, 1139, 1362, 1981
HphI GGTGA 3 cut(s) 442, 553, 985
Hpy166II GTNNAC 5 cut(s) 129, 333, 744, 1294, 1722
Hpy188I TCNGA 9 cut(s) 96, 727, 787, 1300, 1821, 1861, 1903, 1989, 2125
Hpy188III TCNNGA 6 cut(s) 516, 1574, 1715, 1775, 1925, 1946
Hpy8I GTNNAC 5 cut(s) 129, 333, 744, 1294, 1722
Hpy99I CGWCG 2 cut(s) 932, 1037
HpyCH4III ACNGT 6 cut(s) 1074, 1094, 1492, 1530, 1630, 2087
HpyCH4IV ACGT 2 cut(s) 1026, 1272
HpyF10VI GCNNNNNNNGC 9 cut(s) 149, 584, 755, 888, 951, 1600, 1878, 1915, 1974
HpyF3I CTNAG 4 cut(s) 719, 784, 1910, 2093
HpySE526I ACGT 2 cut(s) 1026, 1272
Hsp92I GRCGYC 2 cut(s) 1356, 1816
HspAI GCGC 7 cut(s) 150, 661, 762, 945, 960, 1100, 1918
KspAI GTTAAC 1 cut(s) 744
Kzo9I GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
LmnI GCTCC 2 cut(s) 195, 575
Lsp1109I GCAGC 4 cut(s) 394, 861, 1964, 2056
LweI GCATC 4 cut(s) 574, 793, 1077, 1333
MaeI CTAG 7 cut(s) 54, 336, 675, 1119, 1176, 1247, 1340
MaeII ACGT 2 cut(s) 1026, 1272
MaeIII GTNAC 8 cut(s) 47, 430, 733, 991, 1134, 1335, 1348, 1686
MalI GATC 5 cut(s) 724, 1132, 1374, 1450, 2019
MboI GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
MfeI CAATTG 4 cut(s) 828, 1215, 1265, 1725
MflI RGATCY 2 cut(s) 722, 1372
MhlI GDGCHC 4 cut(s) 87, 158, 1724, 2094
MlyI GAGTC 4 cut(s) 521, 1313, 1781, 1871
MmeI TCCRAC 2 cut(s) 1023, 1132
MnlI CCTC 9 cut(s) 370, 893, 912, 977, 1006, 1040, 1426, 1455, 1578
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 5 cut(s) 417, 743, 1662, 1836, 2076
MslI CAYNNNNRTG 1 cut(s) 1727
MspA1I CMGCKG 2 cut(s) 1739, 2047
MspI CCGG 6 cut(s) 107, 119, 411, 1139, 1362, 1981
MspR9I CCNGG 5 cut(s) 108, 665, 1060, 1650, 2000
MunI CAATTG 4 cut(s) 828, 1215, 1265, 1725
MvaI CCWGG 4 cut(s) 665, 1060, 1650, 2000
MvnI CGCG 2 cut(s) 152, 960
MwoI GCNNNNNNNGC 9 cut(s) 149, 584, 755, 888, 951, 1600, 1878, 1915, 1974
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 2 cut(s) 193, 871
NdeII GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
NlaIV GGNNCC 2 cut(s) 1963, 2004
NmuCI GTSAC 3 cut(s) 430, 991, 1686
NsbI TGCGCA 2 cut(s) 763, 1919
NspI RCATGY 2 cut(s) 1603, 1944
OliI CACNNNNGTG 1 cut(s) 1727
PciI ACATGT 1 cut(s) 1940
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 5 cut(s) 241, 250, 776, 932, 1538
PflMI CCANNNNNTGG 2 cut(s) 670, 2047
PinAI ACCGGT 1 cut(s) 1138
PkrI GCNGC 7 cut(s) 409, 597, 851, 1049, 1610, 1979, 2046
PleI GAGTC 4 cut(s) 520, 1312, 1781, 1870
PpsI GAGTC 4 cut(s) 520, 1312, 1781, 1870
PpuMI RGGWCCY 1 cut(s) 2002
PscI ACATGT 1 cut(s) 1940
PshBI ATTAAT 1 cut(s) 1662
Psp5II RGGWCCY 1 cut(s) 2002
Psp6I CCWGG 4 cut(s) 663, 1058, 1648, 1998
PspEI GGTNACC 1 cut(s) 733
PspFI CCCAGC 2 cut(s) 59, 2040
PspGI CCWGG 4 cut(s) 663, 1058, 1648, 1998
PspN4I GGNNCC 2 cut(s) 1963, 2004
PspPI GGNCC 4 cut(s) 715, 1049, 1886, 2002
PspPPI RGGWCCY 1 cut(s) 2002
PstNI CAGNNNCTG 3 cut(s) 670, 1490, 2047
PsuI RGATCY 2 cut(s) 722, 1372
PvuII CAGCTG 2 cut(s) 1739, 2047
RsaI GTAC 4 cut(s) 29, 1080, 1271, 1278
RsaNI GTAC 4 cut(s) 28, 1079, 1270, 1277
RseI CAYNNNNRTG 1 cut(s) 1727
SaqAI TTAA 5 cut(s) 417, 743, 1662, 1836, 2076
SatI GCNGC 7 cut(s) 408, 596, 850, 1048, 1609, 1978, 2045
Sau3AI GATC 5 cut(s) 722, 1130, 1372, 1448, 2017
Sau96I GGNCC 4 cut(s) 715, 1049, 1886, 2002
SchI GAGTC 4 cut(s) 521, 1313, 1781, 1871
ScrFI CCNGG 5 cut(s) 108, 665, 1060, 1650, 2000
SduI GDGCHC 4 cut(s) 87, 158, 1724, 2094
SfaNI GCATC 4 cut(s) 574, 793, 1077, 1333
SinI GGWCC 1 cut(s) 2002
SmiMI CAYNNNNRTG 1 cut(s) 1727
SmlI CTYRAG 2 cut(s) 311, 884
SmoI CTYRAG 2 cut(s) 311, 884
SpeI ACTAGT 2 cut(s) 335, 1175
SsiI CCGC 7 cut(s) 595, 912, 958, 1047, 1253, 1390, 1609
SspMI CTAG 7 cut(s) 54, 336, 675, 1119, 1176, 1247, 1340
StyD4I CCNGG 5 cut(s) 106, 663, 1058, 1648, 1998
StyI CCWWGG 3 cut(s) 193, 372, 871
TaaI ACNGT 6 cut(s) 1074, 1094, 1492, 1530, 1630, 2087
TaiI ACGT 2 cut(s) 1029, 1275
TaqI TCGA 5 cut(s) 219, 615, 1460, 1810, 1973
TatI WGTACW 2 cut(s) 27, 1078
TauI GCSGC 3 cut(s) 598, 1050, 1611
TfiI GAWTC 5 cut(s) 241, 250, 776, 932, 1538
Tru1I TTAA 5 cut(s) 417, 743, 1662, 1836, 2076
Tru9I TTAA 5 cut(s) 417, 743, 1662, 1836, 2076
TscAI CASTG 3 cut(s) 1024, 1495, 2092
TseFI GTSAC 3 cut(s) 430, 991, 1686
TseI GCWGC 4 cut(s) 407, 849, 1977, 2044
Tsp45I GTSAC 3 cut(s) 430, 991, 1686
TspRI CASTG 3 cut(s) 1024, 1495, 2092
Van91I CCANNNNNTGG 2 cut(s) 670, 2047
VneI GTGCAC 1 cut(s) 1720
VpaK11BI GGWCC 1 cut(s) 2002
VspI ATTAAT 1 cut(s) 1662
XapI RAATTY 3 cut(s) 544, 1108, 1770
XceI RCATGY 2 cut(s) 1603, 1944
XmiI GTMKAC 1 cut(s) 1293
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 7 cut(s) 54, 336, 675, 1119, 1176, 1247, 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.