FvH4_6g26960

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008797
Physical Location & Seq
Forward (+)
20635311 .. 20638426
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26960.t3

Sequence Viewer

Length: 609 bp
ATGAAGAGGAGTTTCATATTGTCAGTCTCCGCCTGTTTCTTTCTGGTCTTGTCTTTCTCCTTTGTTCTCTCACTCTCAAATCCACCTCTTCTTGGAGTTCATCCACTCGATGAAAAGTACTTTGCTCGAGAGGTGATCATGTGCAAAGACGACTCCAAGTCGTTCACTAGAGACCGTCTGAATGATGATTTCTGTGACTGTGTTGATGGAACCGACGAGCCTGGGACTTCTGCTTGTCCTGCAGGCAAATTTTATTGTCGGAATATTGGAAGTACACCACAGTTCATATTTTCTTCTCGAGTCAATGATCGTATTTGTGATTGCTGCGATGGAAGCGATGAGAATGATGGTAGTCTTAATTGTCCAAATACATGCATCATGGGCGGGACTGTTGAGTACAAAACTGACCACCGCTTCTCTAGAGTTCAGGATCTAGGGCATCTTGACACAAAGCAAACCAAAAATGGAGTCACTCTGGATGACTTCATCCAGAAAGTTAAAGGGTTTTTGAAGGTAATAATAATCGTACAATTAGTTATTGCTTCCGTTTTTGTGGTTTGCCGGATATTCCATCGTTGTGCCAGATCAAAACGAAGACGTCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PRKCSH-like

Protein Analysis

203

Amino Acids

22.81

Weight (kDa)

8.41

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 19 - 127 1.4e-30 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 601
AciI CCGC 3 cut(s) 30, 384, 412
AclWI GGATC 1 cut(s) 438
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 598
AfaI GTAC 4 cut(s) 119, 274, 398, 528
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 1 cut(s) 511
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 31, 165
AlwI GGATC 1 cut(s) 438
Ama87I CYCGRG 2 cut(s) 126, 297
ApeKI GCWGC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 2 cut(s) 126, 297
BbsI GAAGAC 1 cut(s) 601
BbvI GCAGC 1 cut(s) 311
BccI CCATC 4 cut(s) 200, 323, 341, 579
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 31, 165
BfaI CTAG 3 cut(s) 168, 420, 434
BfmI CTRYAG 1 cut(s) 240
BisI GCNGC 1 cut(s) 325
BlsI GCNGC 1 cut(s) 326
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 222
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 2 cut(s) 126, 297
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 384, 448
BpiI GAAGAC 1 cut(s) 601
BsaHI GRCGYC 1 cut(s) 598
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 92
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 3 cut(s) 100, 484, 486
BseLI CCNNNNNNNGG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 311
BsiHKCI CYCGRG 2 cut(s) 126, 297
BsiSI CCGG 1 cut(s) 562
BslFI GGGAC 2 cut(s) 238, 400
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 2 cut(s) 31, 165
BsmFI GGGAC 2 cut(s) 238, 400
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 2 cut(s) 126, 297
Bsp143I GATC 4 cut(s) 135, 307, 430, 584
BspACI CCGC 3 cut(s) 30, 384, 412
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 4 cut(s) 135, 307, 430, 584
BssNI GRCGYC 1 cut(s) 598
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 4 cut(s) 176, 200, 282, 391
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 598
BstC8I GCNNGC 1 cut(s) 244
BstF5I GGATG 3 cut(s) 100, 484, 486
BstKTI GATC 4 cut(s) 138, 310, 433, 587
BstMAI GTCTC 2 cut(s) 31, 165
BstMBI GATC 4 cut(s) 135, 307, 430, 584
BstMWI GCNNNNNNNGC 3 cut(s) 239, 333, 381
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 311
BstV2I GAAGAC 1 cut(s) 601
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BtgZI GCGATG 2 cut(s) 342, 351
BtsCI GGATG 3 cut(s) 100, 484, 486
Cac8I GCNNGC 1 cut(s) 244
Csp6I GTAC 4 cut(s) 118, 273, 397, 527
CviAII CATG 3 cut(s) 139, 372, 379
CviJI RGCY 1 cut(s) 220
CviKI_1 RGCY 1 cut(s) 220
CviQI GTAC 4 cut(s) 118, 273, 397, 527
DpnI GATC 4 cut(s) 137, 309, 432, 586
DpnII GATC 4 cut(s) 135, 307, 430, 584
DriI GACNNNNNGTC 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 93
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 165
Eco88I CYCGRG 2 cut(s) 126, 297
EcoRII CCWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 377
FaeI CATG 3 cut(s) 142, 375, 382
FaiI YATR 5 cut(s) 17, 140, 287, 373, 380
FaqI GGGAC 2 cut(s) 238, 400
FatI CATG 3 cut(s) 138, 371, 378
FauI CCCGC 1 cut(s) 377
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 325
FokI GGATG 3 cut(s) 87, 473, 491
Fsp4HI GCNGC 1 cut(s) 325
FspBI CTAG 3 cut(s) 168, 420, 434
GluI GCNGC 1 cut(s) 325
HapII CCGG 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 598
Hin1II CATG 3 cut(s) 142, 375, 382
HinfI GANTC 3 cut(s) 152, 300, 468
HpaII CCGG 1 cut(s) 562
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 2 cut(s) 165, 275
Hpy188I TCNGA 2 cut(s) 180, 261
Hpy188III TCNNGA 7 cut(s) 128, 297, 420, 428, 443, 476, 490
Hpy8I GTNNAC 2 cut(s) 165, 275
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 4 cut(s) 176, 200, 282, 391
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 3 cut(s) 144, 242, 375
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 333, 381
HpySE526I ACGT 1 cut(s) 598
Hsp92I GRCGYC 1 cut(s) 598
Hsp92II CATG 3 cut(s) 142, 375, 382
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 4 cut(s) 135, 307, 430, 584
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 2 cut(s) 384, 448
MaeI CTAG 3 cut(s) 168, 420, 434
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 2 cut(s) 194, 469
MalI GATC 4 cut(s) 137, 309, 432, 586
MboI GATC 4 cut(s) 135, 307, 430, 584
MboII GAAGA 4 cut(s) 16, 80, 285, 606
MflI RGATCY 1 cut(s) 430
MluCI AATT 3 cut(s) 248, 358, 530
MlyI GAGTC 3 cut(s) 146, 309, 477
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 2 cut(s) 96, 124
Mph1103I ATGCAT 1 cut(s) 377
MseI TTAA 3 cut(s) 357, 498, 607
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 1 cut(s) 562
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 239, 333, 381
NdeII GATC 4 cut(s) 135, 307, 430, 584
NlaIII CATG 3 cut(s) 142, 375, 382
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 194, 469
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 375
PaeR7I CTCGAG 2 cut(s) 126, 297
PkrI GCNGC 1 cut(s) 326
PleI GAGTC 3 cut(s) 146, 308, 476
PpsI GAGTC 3 cut(s) 146, 308, 476
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 4 cut(s) 119, 274, 398, 528
RsaNI GTAC 4 cut(s) 118, 273, 397, 527
RseI CAYNNNNRTG 1 cut(s) 576
SaqAI TTAA 3 cut(s) 357, 498, 607
SatI GCNGC 1 cut(s) 325
Sau3AI GATC 4 cut(s) 135, 307, 430, 584
SbfI CCTGCAGG 1 cut(s) 244
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 3 cut(s) 146, 309, 477
ScrFI CCNGG 1 cut(s) 222
SdaI CCTGCAGG 1 cut(s) 244
SetI ASST 4 cut(s) 88, 135, 516, 601
SfaNI GCATC 2 cut(s) 384, 448
SfcI CTRYAG 1 cut(s) 240
Sfr274I CTCGAG 2 cut(s) 126, 297
SlaI CTCGAG 2 cut(s) 126, 297
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 126, 297
SmoI CTYRAG 2 cut(s) 126, 297
Sse8387I CCTGCAGG 1 cut(s) 244
Sse9I AATT 3 cut(s) 248, 358, 530
SsiI CCGC 3 cut(s) 30, 384, 412
SspI AATATT 1 cut(s) 265
SspMI CTAG 3 cut(s) 168, 420, 434
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 4 cut(s) 176, 200, 282, 391
TaiI ACGT 1 cut(s) 601
TaqI TCGA 3 cut(s) 108, 127, 298
TasI AATT 3 cut(s) 248, 358, 530
TatI WGTACW 3 cut(s) 117, 272, 396
Tru1I TTAA 3 cut(s) 357, 498, 607
Tru9I TTAA 3 cut(s) 357, 498, 607
TseFI GTSAC 2 cut(s) 194, 469
TseI GCWGC 1 cut(s) 324
Tsp45I GTSAC 2 cut(s) 194, 469
TspDTI ATGAA 6 cut(s) 4, 17, 89, 126, 274, 475
TspGWI ACGGA 1 cut(s) 535
XapI RAATTY 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 375
XhoI CTCGAG 2 cut(s) 126, 297
XspI CTAG 3 cut(s) 168, 420, 434
ZraI GACGTC 1 cut(s) 599
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.