MD02G1242400.v1.1

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008797
Physical Location & Seq
Reverse (-)
29261388 .. 29264310
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1242400.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGAGACGAGTTTCATCCGTCTTGTCTCCGTGTGTTTCCTACTCACCTCCTCCTTCACCTCTCCCCACTCTCTCCCCATCCCACCTCTTCTTGGAGTTCACCCACTCGATGAAAGCTACTATGGTTCAGAGGTGATCAGCTGCAGGGATCACTCCAAATCCTTCACTAGAGACCGTCTCAATGATGATTTCTGTGACTGCCTTGACGGAACCGATGAGCCTGGGACTTCCGCTTGCCCTGCAGGAAAATTCTATTGTTTGAATAGAGGAAGTACTCCGCGCTTCCTATTTTCTTCTCGAGTTAATGATCATGTTTGTGATTGTTGCGATGGAAGTGACGAGTATGATGGTAGTGTCAATTGTCCAAACACATGCATCATGGGCGGGAATATTGAGTACAAAACCATCCATAGCTTCTCAAGAGTAGGTGGTCTTGGGCAAATTGACAGAAAACAATCCAAAAGTGGAGTTAATTTGGACGACTTGATTCAGAAGCTTACAGGTTTGAAGATATTAATCATCATACAAGTAGTTCTCACTATTACTGTCGTGTTTTTCCGGATTCTCCGCCGTCGTACCAGATCTAAAAGAAGACGTCGTATTGCCATAAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PRKCSH-like

Protein Analysis

206

Amino Acids

22.94

Weight (kDa)

8.46

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 27 - 127 3.1e-31 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 598
AccII CGCG 1 cut(s) 280
AccIII TCCGGA 1 cut(s) 558
AciI CCGC 4 cut(s) 231, 278, 384, 568
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 595
AfaI GTAC 3 cut(s) 274, 398, 577
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 2 cut(s) 262, 508
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 4 cut(s) 117, 141, 414, 496
AluI AGCT 4 cut(s) 117, 141, 414, 496
Alw26I GTCTC 3 cut(s) 31, 165, 182
AlwI GGATC 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 297
Aor13HI TCCGGA 1 cut(s) 558
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 248
AseI ATTAAT 1 cut(s) 515
AspLEI GCGC 1 cut(s) 282
AsuHPI GGTGA 4 cut(s) 37, 49, 92, 145
AvaI CYCGRG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 1 cut(s) 128
BccI CCATC 4 cut(s) 86, 323, 341, 413
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 2 cut(s) 135, 307
BcoDI GTCTC 3 cut(s) 31, 165, 182
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 142, 240
BglII AGATCT 1 cut(s) 581
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 1 cut(s) 222
BmeT110I CYCGRG 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 598
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpuEI CTTGAG 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 595
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 558
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 515
BseAI TCCGGA 1 cut(s) 558
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 3 cut(s) 15, 78, 405
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 1 cut(s) 559
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 3 cut(s) 31, 165, 182
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 238
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 558
Bsp143I GATC 4 cut(s) 135, 148, 307, 581
BspACI CCGC 4 cut(s) 231, 278, 384, 568
BspEI TCCGGA 1 cut(s) 558
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 2 cut(s) 146, 244
BspPI GGATC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 4 cut(s) 135, 148, 307, 581
BssNI GRCGYC 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 176, 547
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 235
BstF5I GGATG 3 cut(s) 15, 78, 405
BstFNI CGCG 1 cut(s) 280
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 4 cut(s) 138, 151, 310, 584
BstMAI GTCTC 3 cut(s) 31, 165, 182
BstMBI GATC 4 cut(s) 135, 148, 307, 581
BstMWI GCNNNNNNNGC 2 cut(s) 239, 381
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 142, 240
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 3 cut(s) 15, 78, 405
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 1 cut(s) 282
Csp6I GTAC 3 cut(s) 273, 397, 576
CviAII CATG 3 cut(s) 311, 372, 379
CviJI RGCY 5 cut(s) 117, 141, 220, 414, 496
CviKI_1 RGCY 5 cut(s) 117, 141, 220, 414, 496
CviQI GTAC 3 cut(s) 273, 397, 576
DpnI GATC 4 cut(s) 137, 150, 309, 583
DpnII GATC 4 cut(s) 135, 148, 307, 581
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 557
Eco31I GGTCTC 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 3 cut(s) 314, 375, 382
FaiI YATR 8 cut(s) 123, 312, 345, 373, 380, 411, 524, 608
FaqI GGGAC 1 cut(s) 238
FatI CATG 3 cut(s) 310, 371, 378
FauI CCCGC 1 cut(s) 377
FbaI TGATCA 2 cut(s) 135, 307
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 3 cut(s) 2, 65, 392
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 281
GluI GCNGC 1 cut(s) 142
HapII CCGG 1 cut(s) 559
HhaI GCGC 1 cut(s) 282
Hin1I GRCGYC 1 cut(s) 595
Hin1II CATG 3 cut(s) 314, 375, 382
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 2 cut(s) 487, 562
HpaII CCGG 1 cut(s) 559
HphI GGTGA 4 cut(s) 37, 49, 92, 145
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 130, 492
Hpy188III TCNNGA 3 cut(s) 297, 420, 559
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 576, 600
HpyAV CCTTC 2 cut(s) 64, 172
HpyCH4III ACNGT 2 cut(s) 176, 547
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 3 cut(s) 144, 242, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 381
HpySE526I ACGT 1 cut(s) 595
Hsp92I GRCGYC 1 cut(s) 595
Hsp92II CATG 3 cut(s) 314, 375, 382
HspAI GCGC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 558
Ksp22I TGATCA 2 cut(s) 135, 307
Kzo9I GATC 4 cut(s) 135, 148, 307, 581
LpnPI CCDG 8 cut(s) 130, 207, 228, 234, 252, 486, 572, 592
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 384
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 2 cut(s) 194, 335
MalI GATC 4 cut(s) 137, 150, 309, 583
MboI GATC 4 cut(s) 135, 148, 307, 581
MboII GAAGA 4 cut(s) 80, 285, 520, 603
MfeI CAATTG 1 cut(s) 358
MflI RGATCY 1 cut(s) 581
MluCI AATT 5 cut(s) 248, 358, 441, 472, 610
MnlI CCTC 6 cut(s) 58, 61, 70, 96, 124, 260
Mph1103I ATGCAT 1 cut(s) 377
MroI TCCGGA 1 cut(s) 558
MseI TTAA 3 cut(s) 303, 471, 515
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 1 cut(s) 559
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 239, 381
NdeII GATC 4 cut(s) 135, 148, 307, 581
NlaIII CATG 3 cut(s) 314, 375, 382
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 194, 335
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 375
PaeR7I CTCGAG 1 cut(s) 297
PfeI GAWTC 2 cut(s) 487, 562
PkrI GCNGC 1 cut(s) 143
PshBI ATTAAT 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 211
PstI CTGCAG 2 cut(s) 146, 244
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 141
RsaI GTAC 3 cut(s) 274, 398, 577
RsaNI GTAC 3 cut(s) 273, 397, 576
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 3 cut(s) 303, 471, 515
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 4 cut(s) 135, 148, 307, 581
SbfI CCTGCAGG 1 cut(s) 244
ScaI AGTACT 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 222
SdaI CCTGCAGG 1 cut(s) 244
SfaNI GCATC 1 cut(s) 384
SfcI CTRYAG 2 cut(s) 142, 240
Sfr274I CTCGAG 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 297, 418
SmoI CTYRAG 2 cut(s) 297, 418
Sse8387I CCTGCAGG 1 cut(s) 244
Sse9I AATT 5 cut(s) 248, 358, 441, 472, 610
SsiI CCGC 4 cut(s) 231, 278, 384, 568
SspI AATATT 1 cut(s) 391
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 176, 547
TaiI ACGT 1 cut(s) 598
TaqI TCGA 2 cut(s) 108, 298
TasI AATT 5 cut(s) 248, 358, 441, 472, 610
TatI WGTACW 2 cut(s) 272, 396
TfiI GAWTC 2 cut(s) 487, 562
Tru1I TTAA 3 cut(s) 303, 471, 515
Tru9I TTAA 3 cut(s) 303, 471, 515
TseFI GTSAC 2 cut(s) 194, 335
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 2 cut(s) 194, 335
TspDTI ATGAA 2 cut(s) 4, 126
TspGWI ACGGA 3 cut(s) 8, 19, 222
VspI ATTAAT 1 cut(s) 515
XapI RAATTY 1 cut(s) 248
XceI RCATGY 1 cut(s) 375
XhoI CTCGAG 1 cut(s) 297
XspI CTAG 1 cut(s) 168
ZraI GACGTC 1 cut(s) 596
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.