Rroxscaffold_2G00115260

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008797
Physical Location & Seq
Reverse (-)
41484879 .. 41487148
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115260.1

Sequence Viewer

Length: 606 bp
ATGGAGACGAGTTTCATATGGTCAGTCTCCGCCTGTTTCTTTCTGGTCTCGTCCTTCTCCTTTGTTCGCTCATTCTCCAATCCATCTCTTCTTGGAGTTCATCCACTCGATGAAAAGTACTTTGCTACAGAGGTGATCAAGTGCAAGGACGACTCCAAGTCGTTCACCAGAGACCGTCTCAATGATAATTTCTGTGACTGTGTTGATGGGACTGACGAGCCTGGGACTTCTGCTTGTCCTGCAGGCAAATTTTATTGTCAGAATATTGGAAGTACACCACAGTTCATATTTTCTTCTCAAGTTAATGATCGTATTTGTGATTGCTGCGATGGAAGTGATGAGAATGATGGTAGTCTCAATTGTCCAAATACATGCATCATGGGCGGGAATATTGAGTACAAAACTGACCACCGCTTCTCTAGAGTTCGGGATCGAGGGTATGTTGACAAAAAGCAAACCGAAAATAGAGTCAGTCTGGATGACTTTGTTCAGAAGCTTAAAGGTTTGAAGATAATAATAATCATACAATTAGTTATTGCTAGTGTTTTTGTGGTTTGCCGGATATTCCATCGTCGTGCCAGATCAAAACGAAGACGTCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PRKCSH-like

Protein Analysis

201

Amino Acids

22.92

Weight (kDa)

8.44

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 27 - 128 1.2e-29 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 598
AciI CCGC 3 cut(s) 30, 384, 412
AclWI GGATC 1 cut(s) 438
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 595
AfaI GTAC 3 cut(s) 119, 274, 398
AgsI TTSAA 1 cut(s) 508
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
Alw26I GTCTC 5 cut(s) 31, 52, 165, 182, 359
AlwI GGATC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AsuHPI GGTGA 2 cut(s) 145, 157
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 1 cut(s) 311
BccI CCATC 5 cut(s) 91, 200, 323, 341, 576
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BfaI CTAG 2 cut(s) 420, 540
BfmI CTRYAG 2 cut(s) 126, 240
BisI GCNGC 1 cut(s) 325
BlsI GCNGC 1 cut(s) 326
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 222
BmeRI GACNNNNNGTC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 598
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpuEI CTTGAG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 595
BsaI GGTCTC 2 cut(s) 52, 165
BsaJI CCNNGG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 2 cut(s) 100, 484
BseXI GCAGC 1 cut(s) 311
BsiSI CCGG 1 cut(s) 559
BslFI GGGAC 2 cut(s) 223, 238
BsmAI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 223, 238
Bso31I GGTCTC 2 cut(s) 52, 165
Bsp143I GATC 4 cut(s) 135, 307, 430, 581
BspACI CCGC 3 cut(s) 30, 384, 412
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 1 cut(s) 438
BspTNI GGTCTC 2 cut(s) 52, 165
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 4 cut(s) 135, 307, 430, 581
BssNI GRCGYC 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 3 cut(s) 176, 200, 282
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 244
BstF5I GGATG 2 cut(s) 100, 484
BstKTI GATC 4 cut(s) 138, 310, 433, 584
BstMAI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BstMBI GATC 4 cut(s) 135, 307, 430, 581
BstMWI GCNNNNNNNGC 2 cut(s) 239, 381
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 126, 240
BstV1I GCAGC 1 cut(s) 311
BstV2I GAAGAC 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 100, 484
Cac8I GCNNGC 1 cut(s) 244
Csp6I GTAC 3 cut(s) 118, 273, 397
CviAII CATG 2 cut(s) 372, 379
CviJI RGCY 2 cut(s) 220, 496
CviKI_1 RGCY 2 cut(s) 220, 496
CviQI GTAC 3 cut(s) 118, 273, 397
DpnI GATC 4 cut(s) 137, 309, 432, 583
DpnII GATC 4 cut(s) 135, 307, 430, 581
DriI GACNNNNNGTC 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 93
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 19
Eco31I GGTCTC 2 cut(s) 52, 165
EcoRII CCWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 375, 382
FaiI YATR 7 cut(s) 17, 19, 287, 373, 380, 441, 524
FaqI GGGAC 2 cut(s) 223, 238
FatI CATG 2 cut(s) 371, 378
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 17
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 325
FokI GGATG 2 cut(s) 87, 491
Fsp4HI GCNGC 1 cut(s) 325
FspBI CTAG 2 cut(s) 420, 540
GluI GCNGC 1 cut(s) 325
HapII CCGG 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 595
Hin1II CATG 2 cut(s) 375, 382
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 2 cut(s) 152, 468
HpaII CCGG 1 cut(s) 559
HphI GGTGA 2 cut(s) 145, 157
Hpy166II GTNNAC 3 cut(s) 165, 275, 445
Hpy188I TCNGA 2 cut(s) 261, 492
Hpy188III TCNNGA 3 cut(s) 420, 428, 476
Hpy8I GTNNAC 3 cut(s) 165, 275, 445
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 3 cut(s) 176, 200, 282
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 3 cut(s) 144, 242, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 381
HpySE526I ACGT 1 cut(s) 595
Hsp92I GRCGYC 1 cut(s) 595
Hsp92II CATG 2 cut(s) 375, 382
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 4 cut(s) 135, 307, 430, 581
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 1 cut(s) 384
MaeI CTAG 2 cut(s) 420, 540
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 1 cut(s) 194
MalI GATC 4 cut(s) 137, 309, 432, 583
MboI GATC 4 cut(s) 135, 307, 430, 581
MboII GAAGA 4 cut(s) 80, 285, 520, 603
MfeI CAATTG 1 cut(s) 358
MluCI AATT 4 cut(s) 187, 248, 358, 527
MlyI GAGTC 2 cut(s) 146, 477
MnlI CCTC 2 cut(s) 124, 428
Mph1103I ATGCAT 1 cut(s) 377
MseI TTAA 3 cut(s) 303, 498, 604
MslI CAYNNNNRTG 1 cut(s) 573
MspI CCGG 1 cut(s) 559
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 239, 381
NdeI CATATG 1 cut(s) 17
NdeII GATC 4 cut(s) 135, 307, 430, 581
NlaIII CATG 2 cut(s) 375, 382
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 375
PkrI GCNGC 1 cut(s) 326
PleI GAGTC 2 cut(s) 146, 476
PpsI GAGTC 2 cut(s) 146, 476
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PstI CTGCAG 1 cut(s) 244
RsaI GTAC 3 cut(s) 119, 274, 398
RsaNI GTAC 3 cut(s) 118, 273, 397
RseI CAYNNNNRTG 1 cut(s) 573
SaqAI TTAA 3 cut(s) 303, 498, 604
SatI GCNGC 1 cut(s) 325
Sau3AI GATC 4 cut(s) 135, 307, 430, 581
SbfI CCTGCAGG 1 cut(s) 244
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 2 cut(s) 146, 477
ScrFI CCNGG 1 cut(s) 222
SdaI CCTGCAGG 1 cut(s) 244
SetI ASST 4 cut(s) 135, 498, 505, 598
SfaNI GCATC 1 cut(s) 384
SfcI CTRYAG 2 cut(s) 126, 240
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse8387I CCTGCAGG 1 cut(s) 244
Sse9I AATT 4 cut(s) 187, 248, 358, 527
SsiI CCGC 3 cut(s) 30, 384, 412
SspI AATATT 2 cut(s) 265, 391
SspMI CTAG 2 cut(s) 420, 540
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 3 cut(s) 176, 200, 282
TaiI ACGT 1 cut(s) 598
TaqI TCGA 2 cut(s) 108, 433
TasI AATT 4 cut(s) 187, 248, 358, 527
TatI WGTACW 3 cut(s) 117, 272, 396
Tru1I TTAA 3 cut(s) 303, 498, 604
Tru9I TTAA 3 cut(s) 303, 498, 604
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 324
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 4 cut(s) 4, 89, 126, 274
XapI RAATTY 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 375
XspI CTAG 2 cut(s) 420, 540
ZraI GACGTC 1 cut(s) 596
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.