Rorug02G0280900

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008797
Physical Location & Seq
Reverse (-)
30292013 .. 30306705
14693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0280900.1

Sequence Viewer

Length: 5709 bp
ATGGCGGCTCCAGATTCCGGCCCCCAGGGCCTGACGCGACGGCCGTCACGGAGCGCCGCGACGACGACGTTTTCGACGGAGGTCTTCGACAACGAGGTCGTCCCCTCCGCCATCGCCCCTATCGCTCCGCTCCTCCGCGTCGCCAACGAAGTCGAGCCCGAGCGCCCTCGCGTCGCCTATCTCTGTCGGTTCTACGCGTTTGAGAAGGCGCACAGATTGGACCCCGGCTCCAGCGGTCGTGGTGTGAGGCAGTTCAAGACCTTGCTCCTGCAGCGATTGGAGAGGGAGAATGCATCAAGTCTTTCTGCTCGAGTTAAAAAGTCTGATGCCAGGGAAATTACAAGCTTCTATCAGCAATATTATGATCACTATGTTAAAGCTGTTCAAGAGGAGCAGGTAGACAGAGCTCAAGTGAGTAAAGCCTACAATACACTTAACCAGCTTTTTGAAGTACTTTGCGGGGTTAACAAGTCAGAGAAAGTTGAAGAAGTTGCTCCTGAGATTATTGCAGCCGCTAGAGATGTTCAAGAGAAGACGGAAATATACGCCCCGTATAATATTCTTCCTTTGGATTCTGCTGGTGCTACACAGTCTGTCATGCAGCTTGACGAGGTTAAGGCCGCTGTGGCTGCACTGTGGAATACTCGTGGTTTGAACTGGCCAAGTGCATTCGAGCAGCACAGACAAAAAGCTAATGATTTGGATCTCCTTGATTGGCTTAGGGCCATGTTTGGGTTCCAGAGAGATAACGTCAGGAACCAGAGGGAGCATTTAATATTGCTACTTGCGAATACGCATATAAGACTTCATCCCAAACCTGAACCTCTTAACAAGCTTGATGATCGAGCTGTTGATGCAGTTCTGGACAAGCTTTTTAAGAACTACAAAACGTGGTGTAAATTTTTGGGTCGAAAACATAGTTTAAGGCTACCTCAAGGTCAGCAAGAAGTACAACAGAGGAAGATACTTTACATGGGTTTATATCTTCTCATATGGGGTGAAGCAGCGAATGTTCGCTTCATGCCTGAATGCCTCTGCTATATTTTCCATAATATGGCATATGAACTCCATGGTCTCTTGGCTGGAAATGTCAGCATTGTCACTGGGGAAAATATAAAGCCTTCTTATGGTGGGGATGATGAGGCCTTTTTGAGGAAGGTTATAACCCCCCTATACCGTGTAATTGAAAAGGAAGCCAAGAAGGGCGGCGGTGGAAAAGCTCCTCACACAGCTTGGTGCAACTATGATGATCTGAATGAATATTTCTGGTCATCTGATTGTTTCTCTCTTGGTTGGCCCATGCGTGATGATGGGGATTTTTTTAAATCAACTCGTGACTTGGCACAGGGAAGAAAGGGCTCTAAAAGAAAATCTGGAAGCACAGGCAAATCATATTTTATTGAAACTCGCACATTTTGGCACATCTTTCGAAGTTTTGATCGAATGTGGACCTTTTATATACTGGCTCTACAGGCTATGCTCATTATTGCATTCAGCAAGGTTTCACCGCTGGATATATTTCAACAGGATGTCCTAAAAGATCTATCAAGTATTTTCATTACAGCAGCATTTCTTCGCGTCCTTCAGAGTATTCTGGATATGGTTTTGAACTTCCCTGGATATCACAGGTGTCGGTTCACAGATGTCCTGAGAAATATTCTGAAGATAGTTGTTAGTCTTGCATGGGCAGTCATTCTTCCACTTTGCTATGTGCATTCCTTCAATGATTCCCCTCAACAAATTGCAGATATACTGTCATTCCTTAAGAACATTCAAGGCGTTCCATCATTATATCTATTGGCTGTTGCAGTATATTTGCTTCCAAATTTACTGACAGCAGTTTTGTTCCTTTTCCCAATGCTCCGTCGTTTTATTGAAAATTCAGACTGGCATATCATCAGGTTCCTCTTGTGGTGGTCACAGCCAAGAATATATGTTGGAAGAGGAATGCATGAAAGTCAATTTGCCCTTCTAAAGTATACTTTTTTCTGGTTGCTACTTTTGGCTTGCAAGTTTACAGTTAGCTATTTTGTCCAGATAAAACCTCTGGTAGAGCCAACAGAAGACATAATGAAGATTCGTCGTATTAATTATGAGTGGCATGAATTTTTCCCTAATGCTGAACATAACTATGGAGCAGTTGTCTCACTCTGGGCACCTGTTATCATGGTTTATTTCATGGACACACAAATTTGGTATGCTATATTTCAAACATTATATGGAGGTATTGTTGGCGCCTTTGATCGCCTAGGAGAGATTCGTACTCTAGGCATGCTAAGATCCCGGTTCCAGTCTCTACCTGGTGCATTCAACACATATCTAGTGCCCTCGGATAAGTCAACAAAAAGGGGATTCTCTTTCTCAAAGAAATTTGCAGAGGCTTCAGCAAGTAGGAGAAGTGAAGCTGCAAAGTTTGCTCAGTTGTGGAATGAAGTGATATGTAGCTTTCGTGAGGAAGATCTCATCAATACGAGCCTGAAAATAATTCAGTGGCCACCTTTCTTGCTTGCTAGCAAAATCCCAATAGCATTAGATATGGCAGCTCAATTCAAAAACAAGGACTCTGATCTCTGGAAGCGGATATGTGGAGATGAATATATGAAGTGTGCTGTGATTGAATGTTATGAGTCTTTCAAACATGTCCTTAAGGCTTTGGTGGTTGGAGAAAATGAGAAAAGGATCATTGGCATCATTATTAAGGAAATTGAAAGTAACATTTCCAAGAATACATTTCTTGATAATTTTAGAATGGCCTCTTTACCCACCCTCTGCCAGAAATTTGTAGAGCTTGTGGTGATCTTGAAAGATGGTGATTCATCCAAGCGATCTGCGGTGGTGCTGCTGCTGCAAGATATGTTGGAAGTTGTCACTCGTGATATGATGGTGAATGAGATCCGAGAGGTGGTTGAAGTTGGTCATAGTAGCAAGGACACTGGAAGGCAACTTTTTGCTGGTACTGATGCAAAACCTGCGATAGTGTTCCCTCCTCCAGTTACAGCACAATGGGAAGAACAGATAAGGCGCCTCCATCTGCTGTTGACTGTTAAGGAGTCTGCAATTGATGTACCAATAAACCTTGAAGCACGTAGAAGGATTTCATTCTTTGCAAATTCATTGTTCATGGATATGCCCCGTGCTCCCCGTGTTCGTAAGATGCTTTCGTTCAGCATTTTGACTCCCTACTATAGTGAGGAGACTGTGTATTCCAAAAGTGACCTTGAGATGGAAAATGAAGATGGTGTATCAATCATATATTACTTGCAGAAGATTTTCCCAGATGAATGGACTAACTTCATGGAGCGGCTTAATTGTAAAAAGGATGCTGAAATATGGGAAAATGAAGAGAACATTTTGCAGCTGCGTCATTGGGTCTCCTTACGAGGACAAACTCTCTGCAGGACAGTTAGAGGAATGATGTATTACCGACGAGCTCTGAAGCTTCAGGCTTTTCTTGATATGGCTAATGAAGAAGAGATACTAGAAGGGTACAAAGCCGTCACAGTTTCACCAGAGGATGAAAAGAAGAGCCGGAGATCCCTATTTGCTCAACTAGAAGCAGTGGCTGATCTTAAATTTACCTATGTTGCTACTTGCCAAAACTATGGCAATCAGAAGCGAAGTGGAGATCGTCGTGCAACTGACATCCTGAATCTGATGGTTAATAATCCCTCTCTCAGGGTTGCATATATTGATGAAGTTGAAGAACGAGAAGGTGAAAAGGTGCAAAAGGTTTACTATTCTGTATTGGTCAAAGCAGTTGACAACCTTGACCAGGAAATATATCGTATAAAATTGCCGGGCTCAGCAAAGATTGGAGAAGGGAAGCCTGAAAACCAGAACCATGCAGTAATCTTTACTCGTGGAGAAGCTCTTCAGGCTATTGACATGAATCAGGACAATTACTTGGAAGAGGCTCTTAAGATGCGTAACCTTCTTGAAGAATTTAATGAGGATCACGGAGTGCGTTCGCCTTCAATTTTAGGTGTTCGTGAGCATATCTTTACTGGAAGTGTTTCTTCGTTGGCGTGGTTTATGTCAAATCAAGAAATGAGCTTTGTGACCATTGGTCAAAGAGTTCTTGCTAGACCTCTTAAGGTTCGGTTCCATTATGGACATCCAGATGTCTTTGATAGAATCTTCCATATTACTCGTGGTGGCATGAGCAAGGCTTCTCGTGGGATCAATCTCAGCGAGGATATTTTTGCAGGTTTCAATTCCACTCTAAGAAGAGGAAATGTCACTCACCATGAGTACATCCAAGTTGGGAAGGGTAGGGATGTTGGCCTGAATCAAATCTCTCTCTTTGAGGCTAAAGTTGCTTGCGGTAATGGAGAGCAGACACTTAGCAGAGACATCTATCGATTAGGGCACCGTTATGATTTCTTCCGCATGTTGTCGTATTACTTTTCAACAATAGGATTTTATATCAGCTCAATGTTGGTTGTGATGACGGTCTACTCTTTCCTGTATGGTAGACTCTACTTGTCATTGAGTGGAATGGAGAAGACAATTATAAACTATGCTGCCAGAAGGGGAAATAATGCTCTACAAACAGCTATGGCTTCACAATCTGTTGTCCAATTAGGTTTGTTGACGTCCTTGCCCATGATCATGGAAATCGGACTTGAAAGAGGGTTCAGAACTGCATTAGGGGACACGATAATAATGCAGCTGCAGCTAGCATCAGTTTTCTTTACTTTCTCACTGGGTACAAAGGTTCATTACTATGGGAGAACAATCCTGCATGGTGGGGCTAAGTACAGAGCAACTGGTCGTGGCTTTGTTGTTCGGCATGAAAGGTTTGGTGAAAACTACAGGATGTACTCACGGAGTCACTTTACAAAAGGCCTGGAGCTTATGATACTGCTTATTATTTATAGTATATATGGCTCAGCAGCAGATGGCTCAGCTGCATATCTCTTCATCACATTCTCCATGTGGTTCTTAGTGATTTCTTGGTTATTTGCCCCCTTCCTCTTCAACCCTTCGGGATTTGAATGGCAAAAAATAGTGGAAGATTGGGATGATTGGTCAAAATGGATAACTAGCCGTGGTGGTATTGGTGTGCCAGCAAATAAGAGCTGGGAGTCCTGGTGGGATGAGGAACAGGAGCACCTGCAGCACACTGGGATGTTGGGACGGTTTTGGGAGATTGTACTTTCTCTGCGCTTCTTTCTTTTCCAATATGGAATTGTCTACCATCTCAATGTTTCCAGGGGTGATAAAAGTATCATGGTTTATGCTCTGTCATGGCTCGTCATTGTTGCTGTGATGATTATCCTCAAGATTGTATCCTTGGGTAGAAAAAGGTTCAGTGCTGATTTCCAGCTGATGTTCAGACTTCTGAAGTTGTTCGTCTTCATTGGGTTTGTCGTCACCATTGCAGTACTTTTTATTTTCCTTAGTCTCACTGTTGGTGACATCTTTGTAAGCTTACTGGCCTTCTTGCCTACTGGGTGGGCAATACTTATGATATCACAAGCATGCAGGCCATTGGTGAAGGTCTTAGGAATGTGGGGGTCTGTAAAAGCTCTAGCAAGAGGGTACGAGTACGTGATGGGACTGGTTATCTTTACCCCAGTGGCTGTTCTGGCATGGTTCCCATTTGTCTCAGAGTTCCAGACCAGGCTGCTATTCAACCAGGCTTTCAGTCGAGGCCTTCAGATCCAACGTATTCTTTCTGGTGGGAAGAAGCAAAAGTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PRKCSH-like

Protein Analysis

1902

Amino Acids

218.54

Weight (kDa)

9.03

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 41 - 162 1.3e-12 Vta1 like
FKS1_dom1 PF14288 321 - 432 8.1e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 832 - 1015 9.7e-68 Callose synthase 1 domain
Glucan_synthase PF02364 1023 - 1107 8e-27 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1113 - 1716 1.2e-206 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1163, 4514
AarI CACCTGC 1 cut(s) 5124
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 1 cut(s) 4598
Acc36I ACCTGC 4 cut(s) 385, 2977, 4196, 5124
AccB1I GGYRCC 4 cut(s) 2155, 2234, 3021, 4368
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccBSI CCGCTC 2 cut(s) 130, 3301
AccI GTMKAC 5 cut(s) 399, 1979, 4455, 4474, 5196
AccII CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
AclWI GGATC 8 cut(s) 711, 2274, 2687, 2887, 3528, 3960, 4187, 5659
AcoI YGGCCR 3 cut(s) 41, 659, 2492
AcuI CTGAAG 8 cut(s) 1568, 1682, 2367, 3425, 3455, 3857, 5366, 5645
AcyI GRCGYC 3 cut(s) 2235, 3022, 4595
AdeI CACNNNGTG 1 cut(s) 3961
AfiI CCNNNNNNNGG 9 cut(s) 17, 732, 1054, 1127, 1152, 2902, 3223, 3675, 3772
AflII CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
AflIII ACRYGT 2 cut(s) 195, 2638
AhdI GACNNNNNGTC 1 cut(s) 4065
Alw21I GWGCWC 4 cut(s) 409, 3139, 3433, 5115
Alw26I GTCTC 8 cut(s) 1079, 2149, 2298, 3188, 3376, 4344, 5411, 5614
AlwI GGATC 8 cut(s) 711, 2274, 2687, 2887, 3528, 3960, 4187, 5659
AlwNI CAGNNNCTG 3 cut(s) 31, 3563, 5585
Ama87I CYCGRG 2 cut(s) 158, 309
ArsI GACNNNNNNTTYG 4 cut(s) 55, 87, 1825, 1857
AseI ATTAAT 1 cut(s) 2088
Asp700I GAANNNNTTC 5 cut(s) 3094, 3269, 3870, 4012, 5351
AspA2I CCTAGG 1 cut(s) 2248
AspLEI GCGC 6 cut(s) 56, 165, 211, 2237, 3024, 5169
AspS9I GGNCC 6 cut(s) 20, 28, 220, 723, 1296, 1449
AsuC2I CCSGG 3 cut(s) 225, 2284, 3798
AsuII TTCGAA 1 cut(s) 1429
AsuNHI GCTAGC 2 cut(s) 2510, 4678
AvaI CYCGRG 2 cut(s) 158, 309
AvaII GGWCC 2 cut(s) 220, 1449
AvrII CCTAGG 1 cut(s) 2248
BaeGI GKGCMC 3 cut(s) 2158, 2328, 4371
BaeI ACNNNNGTAYC 2 cut(s) 4701, 4734
BalI TGGCCA 2 cut(s) 661, 2494
BanI GGYRCC 4 cut(s) 2155, 2234, 3021, 4368
BanII GRGCYC 5 cut(s) 159, 409, 1361, 3433, 3803
BarI GAAGNNNNNNTAC 6 cut(s) 953, 985, 1803, 1835, 3459, 3491
BauI CACGAG 6 cut(s) 645, 1332, 2871, 3858, 4149, 4173
BbsI GAAGAC 5 cut(s) 76, 539, 2070, 4511, 5350
Bbv12I GWGCWC 4 cut(s) 409, 3139, 3433, 5115
BceAI ACGGC 4 cut(s) 28, 56, 3479, 5034
BcgI CGANNNNNNTGC 4 cut(s) 1409, 1443, 4648, 4682
BciVI GTATCC 1 cut(s) 5302
BclI TGATCA 2 cut(s) 364, 4608
BcnI CCSGG 3 cut(s) 225, 2284, 3798
BcoDI GTCTC 8 cut(s) 1079, 2149, 2298, 3188, 3376, 4344, 5411, 5614
BfmI CTRYAG 7 cut(s) 269, 1469, 3184, 3394, 4673, 4813, 5117
BfoI RGCGCY 4 cut(s) 57, 166, 2238, 3025
BfrI CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
BfuAI ACCTGC 4 cut(s) 385, 2977, 4196, 5124
BfuI GTATCC 1 cut(s) 5302
BglI GCCNNNNNGGC 2 cut(s) 27, 626
BglII AGATCT 2 cut(s) 1540, 2458
BlnI CCTAGG 1 cut(s) 2248
BlpI GCTNAGC 3 cut(s) 3802, 4891, 4906
BmcAI AGTACT 2 cut(s) 453, 5388
Bme18I GGWCC 2 cut(s) 220, 1449
BmeRI GACNNNNNGTC 1 cut(s) 4065
BmeT110I CYCGRG 2 cut(s) 158, 309
BmgT120I GGNCC 6 cut(s) 20, 28, 220, 723, 1296, 1449
BmrI ACTGGG 5 cut(s) 1113, 4715, 5136, 5463, 5573
BmsI GCATC 9 cut(s) 302, 316, 844, 2697, 2950, 3144, 3310, 3912, 4691
BmtI GCTAGC 2 cut(s) 2514, 4682
BmuI ACTGGG 5 cut(s) 1113, 4715, 5136, 5463, 5573
BoxI GACNNNNGTC 2 cut(s) 43, 80
BpiI GAAGAC 5 cut(s) 76, 539, 2070, 4511, 5350
BpmI CTGGAG 3 cut(s) 214, 2973, 4871
Bpu10I CCTNAGC 1 cut(s) 719
Bpu1102I GCTNAGC 3 cut(s) 3802, 4891, 4906
Bpu14I TTCGAA 1 cut(s) 1429
BpuEI CTTGAG 4 cut(s) 393, 918, 3239, 5267
BpuMI CCSGG 3 cut(s) 225, 2284, 3798
Bsa29I ATCGAT 1 cut(s) 4360
BsaAI YACGTR 2 cut(s) 3086, 5554
BsaBI GATNNNNATC 2 cut(s) 702, 5276
BsaHI GRCGYC 3 cut(s) 2235, 3022, 4595
BsaI GGTCTC 2 cut(s) 1079, 3376
Bsc4I CCNNNNNNNGG 9 cut(s) 17, 732, 1054, 1127, 1152, 2902, 3223, 3675, 3772
Bse3DI GCAATG 1 cut(s) 5379
Bse8I GATNNNNATC 2 cut(s) 702, 5276
BseCI ATCGAT 1 cut(s) 4360
BseJI GATNNNNATC 2 cut(s) 702, 5276
BseLI CCNNNNNNNGG 9 cut(s) 17, 732, 1054, 1127, 1152, 2902, 3223, 3675, 3772
BseMI GCAATG 1 cut(s) 5379
BseMII CTCAG 9 cut(s) 489, 1640, 2432, 3688, 3816, 4201, 4905, 4920, 5625
BseRI GAGGAG 5 cut(s) 122, 404, 1212, 2976, 3206
BseSI GKGCMC 3 cut(s) 2158, 2328, 4371
BseX3I CGGCCG 1 cut(s) 41
BseYI CCCAGC 1 cut(s) 5082
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
Bsh1285I CGRYCG 2 cut(s) 44, 238
BshNI GGYRCC 4 cut(s) 2155, 2234, 3021, 4368
BshVI ATCGAT 1 cut(s) 4360
BsiEI CGRYCG 2 cut(s) 44, 238
BsiHKAI GWGCWC 4 cut(s) 409, 3139, 3433, 5115
BsiHKCI CYCGRG 2 cut(s) 158, 309
BsiSI CCGG 5 cut(s) 18, 225, 2284, 3529, 3797
BslFI GGGAC 4 cut(s) 86, 4667, 5151, 5574
BslI CCNNNNNNNGG 9 cut(s) 17, 732, 1054, 1127, 1152, 2902, 3223, 3675, 3772
BsmAI GTCTC 8 cut(s) 1079, 2149, 2298, 3188, 3376, 4344, 5411, 5614
BsmFI GGGAC 4 cut(s) 86, 4667, 5151, 5574
BsmI GAATGC 7 cut(s) 295, 668, 1034, 1490, 1714, 1953, 2306
Bso31I GGTCTC 2 cut(s) 1079, 3376
BsoBI CYCGRG 2 cut(s) 158, 309
Bsp119I TTCGAA 1 cut(s) 1429
Bsp1720I GCTNAGC 3 cut(s) 3802, 4891, 4906
Bsp19I CCATGG 1 cut(s) 1069
BspCNI CTCAG 9 cut(s) 490, 1641, 2431, 3687, 3815, 4200, 4904, 4919, 5624
BspDI ATCGAT 1 cut(s) 4360
BspFNI CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
BspMAI CTGCAG 4 cut(s) 273, 3398, 4677, 5121
BspMI ACCTGC 4 cut(s) 385, 2977, 4196, 5124
BspOI GCTAGC 2 cut(s) 2514, 4682
BspPI GGATC 8 cut(s) 711, 2274, 2687, 2887, 3528, 3960, 4187, 5659
BspQI GCTCTTC 2 cut(s) 3518, 3876
BspT104I TTCGAA 1 cut(s) 1429
BspT107I GGYRCC 4 cut(s) 2155, 2234, 3021, 4368
BspTI CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
BspTNI GGTCTC 2 cut(s) 1079, 3376
BsrBI CCGCTC 2 cut(s) 130, 3301
BsrDI GCAATG 1 cut(s) 5379
BssNAI GTATAC 1 cut(s) 1980
BssNI GRCGYC 3 cut(s) 2235, 3022, 4595
BssSI CACGAG 6 cut(s) 645, 1332, 2871, 3858, 4149, 4173
BssT1I CCWWGG 3 cut(s) 1069, 2248, 5295
Bst1107I GTATAC 1 cut(s) 1980
Bst2BI CACGAG 6 cut(s) 645, 1332, 2871, 3858, 4149, 4173
Bst6I CTCTTC 9 cut(s) 1936, 3336, 3465, 3518, 3876, 3903, 4222, 4925, 4982
BstACI GRCGYC 3 cut(s) 2235, 3022, 4595
BstAFI CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
BstAPI GCANNNNNTGC 2 cut(s) 2414, 2969
BstBAI YACGTR 2 cut(s) 3086, 5554
BstBI TTCGAA 1 cut(s) 1429
BstC8I GCNNGC 9 cut(s) 2008, 2273, 2508, 2512, 4321, 4680, 5070, 5485, 5489
BstDSI CCRYGG 2 cut(s) 1069, 5050
BstENI CCTNNNNNAGG 3 cut(s) 1150, 3673, 3770
BstFNI CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
BstH2I RGCGCY 4 cut(s) 57, 166, 2238, 3025
BstHHI GCGC 6 cut(s) 56, 165, 211, 2237, 3024, 5169
BstMAI GTCTC 8 cut(s) 1079, 2149, 2298, 3188, 3376, 4344, 5411, 5614
BstMCI CGRYCG 2 cut(s) 44, 238
BstNSI RCATGY 4 cut(s) 2275, 2642, 4393, 5487
BstPAI GACNNNNGTC 2 cut(s) 43, 80
BstSFI CTRYAG 7 cut(s) 269, 1469, 3184, 3394, 4673, 4813, 5117
BstSLI GKGCMC 3 cut(s) 2158, 2328, 4371
BstUI CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
BstV2I GAAGAC 5 cut(s) 76, 539, 2070, 4511, 5350
BstX2I RGATCY 7 cut(s) 703, 1540, 2279, 2458, 2892, 3533, 5664
BstXI CCANNNNNNTGG 3 cut(s) 3282, 3602, 4612
BstYI RGATCY 7 cut(s) 703, 1540, 2279, 2458, 2892, 3533, 5664
BstZ17I GTATAC 1 cut(s) 1980
BstZI CGGCCG 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 4360
BsuI GTATCC 1 cut(s) 5302
BsuTUI ATCGAT 1 cut(s) 4360
BtgI CCRYGG 2 cut(s) 1069, 5050
BtgZI GCGATG 1 cut(s) 97
BtsI GCAGTG 1 cut(s) 3564
BveI ACCTGC 4 cut(s) 385, 2977, 4196, 5124
Cac8I GCNNGC 9 cut(s) 2008, 2273, 2508, 2512, 4321, 4680, 5070, 5485, 5489
CaiI CAGNNNCTG 3 cut(s) 31, 3563, 5585
CfoI GCGC 6 cut(s) 56, 165, 211, 2237, 3024, 5169
Cfr13I GGNCC 6 cut(s) 20, 28, 220, 723, 1296, 1449
ClaI ATCGAT 1 cut(s) 4360
CseI GACGC 5 cut(s) 43, 127, 160, 1567, 3350
CsiI ACCWGGT 1 cut(s) 2299
CspCI CAANNNNNGTGG 2 cut(s) 2484, 2519
DinI GGCGCC 2 cut(s) 2236, 3023
DraI TTTAAA 1 cut(s) 1324
DraIII CACNNNGTG 1 cut(s) 3961
DrdI GACNNNNNNGTC 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 4065
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 3 cut(s) 41, 659, 2492
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 9 cut(s) 1936, 3336, 3465, 3518, 3876, 3903, 4222, 4925, 4982
Eam1105I GACNNNNNGTC 1 cut(s) 4065
EarI CTCTTC 9 cut(s) 1936, 3336, 3465, 3518, 3876, 3903, 4222, 4925, 4982
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 2 cut(s) 407, 3431
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 1069, 2248, 5295
Eco147I AGGCCT 3 cut(s) 1145, 4848, 5658
Eco24I GRGCYC 5 cut(s) 159, 409, 1361, 3433, 3803
Eco31I GGTCTC 2 cut(s) 1079, 3376
Eco32I GATATC 2 cut(s) 1622, 5475
Eco47I GGWCC 2 cut(s) 220, 1449
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 2 cut(s) 407, 3431
Eco57I CTGAAG 8 cut(s) 1568, 1682, 2367, 3425, 3455, 3857, 5366, 5645
Eco88I CYCGRG 2 cut(s) 158, 309
EcoICRI GAGCTC 2 cut(s) 407, 3431
EcoNI CCTNNNNNAGG 3 cut(s) 1150, 3673, 3770
EcoO109I RGGNCCY 1 cut(s) 28
EcoRV GATATC 2 cut(s) 1622, 5475
EcoT14I CCWWGG 3 cut(s) 1069, 2248, 5295
EcoT22I ATGCAT 2 cut(s) 295, 1953
EcoT38I GRGCYC 5 cut(s) 159, 409, 1361, 3433, 3803
EgeI GGCGCC 2 cut(s) 2236, 3023
EheI GGCGCC 2 cut(s) 2236, 3023
ErhI CCWWGG 3 cut(s) 1069, 2248, 5295
FalI AAGNNNNNCTT 4 cut(s) 3900, 3932, 4000, 4032
FaqI GGGAC 4 cut(s) 86, 4667, 5151, 5574
FauI CCCGC 1 cut(s) 452
FauNDI CATATG 2 cut(s) 992, 1060
FbaI TGATCA 2 cut(s) 364, 4608
FblI GTMKAC 5 cut(s) 399, 1979, 4455, 4474, 5196
FriOI GRGCYC 5 cut(s) 159, 409, 1361, 3433, 3803
GlaI GCGC 6 cut(s) 55, 164, 210, 2236, 3023, 5168
GsaI CCCAGC 1 cut(s) 5086
GsuI CTGGAG 3 cut(s) 214, 2973, 4871
HaeII RGCGCY 4 cut(s) 57, 166, 2238, 3025
HapII CCGG 5 cut(s) 18, 225, 2284, 3529, 3797
HgaI GACGC 5 cut(s) 43, 127, 160, 1567, 3350
HhaI GCGC 6 cut(s) 56, 165, 211, 2237, 3024, 5169
Hin1I GRCGYC 3 cut(s) 2235, 3022, 4595
Hin6I GCGC 6 cut(s) 54, 163, 209, 2235, 3022, 5167
HinP1I GCGC 6 cut(s) 54, 163, 209, 2235, 3022, 5167
HincII GTYRAC 5 cut(s) 466, 2340, 3039, 3760, 4593
HindII GTYRAC 5 cut(s) 466, 2340, 3039, 3760, 4593
HindIII AAGCTT 5 cut(s) 343, 833, 869, 3437, 5431
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 5 cut(s) 18, 225, 2284, 3529, 3797
HpyCH4IV ACGT 7 cut(s) 68, 750, 890, 3085, 4595, 5553, 5671
HpySE526I ACGT 7 cut(s) 68, 750, 890, 3085, 4595, 5553, 5671
Hsp92I GRCGYC 3 cut(s) 2235, 3022, 4595
HspAI GCGC 6 cut(s) 54, 163, 209, 2235, 3022, 5167
KasI GGCGCC 2 cut(s) 2234, 3021
Ksp22I TGATCA 2 cut(s) 364, 4608
KspAI GTTAAC 1 cut(s) 466
LguI GCTCTTC 2 cut(s) 3518, 3876
LweI GCATC 9 cut(s) 302, 316, 844, 2697, 2950, 3144, 3310, 3912, 4691
MabI ACCWGGT 1 cut(s) 2299
MaeII ACGT 7 cut(s) 68, 750, 890, 3085, 4595, 5553, 5671
MbiI CCGCTC 2 cut(s) 130, 3301
MfeI CAATTG 1 cut(s) 3057
MflI RGATCY 7 cut(s) 703, 1540, 2279, 2458, 2892, 3533, 5664
MlsI TGGCCA 2 cut(s) 661, 2494
MluI ACGCGT 1 cut(s) 195
MluNI TGGCCA 2 cut(s) 661, 2494
Mly113I GGCGCC 2 cut(s) 2235, 3022
MlyI GAGTC 7 cut(s) 2555, 2636, 3059, 3169, 4470, 4840, 5096
MmeI TCCRAC 4 cut(s) 1918, 2641, 2838, 5692
Mox20I TGGCCA 2 cut(s) 661, 2494
Mph1103I ATGCAT 2 cut(s) 295, 1953
MroXI GAANNNNTTC 5 cut(s) 3094, 3269, 3870, 4012, 5351
MscI TGGCCA 2 cut(s) 661, 2494
MslI CAYNNNNRTG 9 cut(s) 2130, 3125, 4119, 4374, 4610, 4725, 5129, 5205, 5482
Msp20I TGGCCA 2 cut(s) 661, 2494
MspA1I CMGCKG 7 cut(s) 234, 623, 1510, 3358, 4672, 4910, 5329
MspCI CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
MspI CCGG 5 cut(s) 18, 225, 2284, 3529, 3797
MunI CAATTG 1 cut(s) 3057
Mva1269I GAATGC 7 cut(s) 295, 668, 1034, 1490, 1714, 1953, 2306
MvnI CGCG 6 cut(s) 37, 59, 138, 171, 197, 1578
NarI GGCGCC 2 cut(s) 2235, 3022
NciI CCSGG 3 cut(s) 225, 2284, 3798
NcoI CCATGG 1 cut(s) 1069
NdeI CATATG 2 cut(s) 992, 1060
NheI GCTAGC 2 cut(s) 2510, 4678
NsiI ATGCAT 2 cut(s) 295, 1953
NspI RCATGY 4 cut(s) 2275, 2642, 4393, 5487
NspV TTCGAA 1 cut(s) 1429
PaeI GCATGC 2 cut(s) 2275, 5487
PaeR7I CTCGAG 1 cut(s) 309
PaqCI CACCTGC 1 cut(s) 5124
PasI CCCWGGG 1 cut(s) 25
PceI AGGCCT 3 cut(s) 1145, 4848, 5658
PciI ACATGT 1 cut(s) 2638
PciSI GCTCTTC 2 cut(s) 3518, 3876
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 71
PctI GAATGC 7 cut(s) 295, 668, 1034, 1490, 1714, 1953, 2306
PdmI GAANNNNTTC 5 cut(s) 3094, 3269, 3870, 4012, 5351
PflMI CCANNNNNTGG 1 cut(s) 1054
PleI GAGTC 7 cut(s) 2555, 2635, 3058, 3169, 4470, 4839, 5095
PluTI GGCGCC 2 cut(s) 2238, 3025
PpsI GAGTC 7 cut(s) 2555, 2635, 3058, 3169, 4470, 4839, 5095
Ppu21I YACGTR 2 cut(s) 3086, 5554
PscI ACATGT 1 cut(s) 2638
PshAI GACNNNNGTC 2 cut(s) 43, 80
PshBI ATTAAT 1 cut(s) 2088
PsiI TTATAA 2 cut(s) 1163, 4514
Psp124BI GAGCTC 2 cut(s) 409, 3433
PspFI CCCAGC 1 cut(s) 5082
PspPI GGNCC 6 cut(s) 20, 28, 220, 723, 1296, 1449
PspXI VCTCGAGB 1 cut(s) 309
PstI CTGCAG 4 cut(s) 273, 3398, 4677, 5121
PstNI CAGNNNCTG 3 cut(s) 31, 3563, 5585
PsuI RGATCY 7 cut(s) 703, 1540, 2279, 2458, 2892, 3533, 5664
PvuII CAGCTG 4 cut(s) 3358, 4672, 4910, 5329
RseI CAYNNNNRTG 9 cut(s) 2130, 3125, 4119, 4374, 4610, 4725, 5129, 5205, 5482
SacI GAGCTC 2 cut(s) 409, 3433
SapI GCTCTTC 2 cut(s) 3518, 3876
Sau96I GGNCC 6 cut(s) 20, 28, 220, 723, 1296, 1449
ScaI AGTACT 2 cut(s) 453, 5388
SchI GAGTC 7 cut(s) 2555, 2636, 3059, 3169, 4470, 4840, 5096
SexAI ACCWGGT 1 cut(s) 2299
SfaNI GCATC 9 cut(s) 302, 316, 844, 2697, 2950, 3144, 3310, 3912, 4691
SfcI CTRYAG 7 cut(s) 269, 1469, 3184, 3394, 4673, 4813, 5117
SfiI GGCCNNNNNGGCC 1 cut(s) 27
SfoI GGCGCC 2 cut(s) 2236, 3023
Sfr274I CTCGAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 1429
SinI GGWCC 2 cut(s) 220, 1449
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 9 cut(s) 2130, 3125, 4119, 4374, 4610, 4725, 5129, 5205, 5482
SmlI CTYRAG 9 cut(s) 309, 408, 933, 1763, 2645, 3218, 3917, 4091, 5282
SmoI CTYRAG 9 cut(s) 309, 408, 933, 1763, 2645, 3218, 3917, 4091, 5282
SphI GCATGC 2 cut(s) 2275, 5487
SseBI AGGCCT 3 cut(s) 1145, 4848, 5658
SspDI GGCGCC 2 cut(s) 2234, 3021
SspI AATATT 5 cut(s) 359, 559, 777, 1262, 1657
SstI GAGCTC 2 cut(s) 409, 3433
StuI AGGCCT 3 cut(s) 1145, 4848, 5658
StyI CCWWGG 3 cut(s) 1069, 2248, 5295
TaiI ACGT 7 cut(s) 71, 753, 893, 3088, 4598, 5556, 5674
TatI WGTACW 7 cut(s) 451, 949, 4251, 4758, 4821, 5155, 5386
TauI GCSGC 6 cut(s) 8, 59, 515, 623, 1209, 3304
TspGWI ACGGA 6 cut(s) 64, 92, 551, 1853, 3972, 4843
Van91I CCANNNNNTGG 1 cut(s) 1054
Vha464I CTTAAG 4 cut(s) 1763, 2645, 3917, 4091
VpaK11BI GGWCC 2 cut(s) 220, 1449
VspI ATTAAT 1 cut(s) 2088
XagI CCTNNNNNAGG 3 cut(s) 1150, 3673, 3770
XceI RCATGY 4 cut(s) 2275, 2642, 4393, 5487
XcmI CCANNNNNNNNNTGG 2 cut(s) 2297, 4148
XhoI CTCGAG 1 cut(s) 309
XmaJI CCTAGG 1 cut(s) 2248
XmiI GTMKAC 5 cut(s) 399, 1979, 4455, 4474, 5196
XmnI GAANNNNTTC 5 cut(s) 3094, 3269, 3870, 4012, 5351
ZraI GACGTC 1 cut(s) 4596
ZrmI AGTACT 2 cut(s) 453, 5388
Zsp2I ATGCAT 2 cut(s) 295, 1953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.