FvH4_6g27130

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013508
Physical Location & Seq
Reverse (-)
20832405 .. 20836360
3956 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27130.t1

Sequence Viewer

Length: 846 bp
ATGGAGCAGGAGCGGAGGACATTGAATGATCAGGACGACGCCGAAGCTGAGGCACTGGAGCTCGTGCTTTTTCAAGTCCCTGAATGCTACGTCTACTTGATACCGCCGAGAAAAAGTGCAGCTTCATACAGGGCTGATGAATGGGATGTAAACAAATGGGTCTGGGAAGGAATACTGAAAGTCATTAGCAAAGGAGAAGATTGCATTATCAAGCTTGAAGAGAAGAACACTGGTGAACTGTATGCTCGGGCATTTTTAAGAAAAGGAGAGCCTCATCCAGTGGAACCCGTAATTGATAGCAGCAGATACTTTGTTCTTCGTGTAGAGGAGAACATTGATGGTCGACTTCGGCATGCTTTCATTGGCTTAGGGTTTCGAGAAAGAACAGAAGCTTATGATTTCCAAGCAGCCCTGCATGATCACATGAAATATCTAAACAAGAAGAAAACAGCAGAAGAGATGGAACAGAAATTTCAGCAAACTTCATCAGTTGATTACAGCTTAAAAGAGGGGGAGACTCTTGTACTGCAATTAAAAAGTAAAAGTTCGGAAGGTGTGAAGTCCAGGCTCTCAGAGCATGGGCTAAGCAATTCACCAACCGGGAAAAAGAGTAACCAAAAAGAGTCCTTAATCAGTATCAAACCTCCACCACCTCCTCCAGGACCTCTCTCACCTACTACGAATGTCCTGCAGTCTTCCTCAAGCCCACCAAATCTGACTCTCGATGGAGCTCCTAAACTTCCAGAATCAGGGGCAGATGATTCAAAAGAAACAAATGTTACCGCAAATCAAAGCACACAAGATATACCAGATGACGACTTTGGGGATTTTCAGGCAGCCGTTTAA

Protein Analysis

282

Amino Acids

31.49

Weight (kDa)

5.04

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 20 - 179 1.2e-52 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58600
fragaria_vesca FvH4_6g27130
malus_domestica MD09G1239500.v1.1 MD17G1232600.v1.1
prunus_persica Prupe.2G061900_v2.0.a1 Prupe.2G061900_v2.0.a1
pyrus_communis pycom17g23530 pycom17g23550
rosa_chinensis RchiOBHm_Chr2g0128491 RchiOBHm_Chr2g0129091
rosa_laevigata RLG00000019054
rosa_roxburghii Rroxscaffold_2G00114880
rosa_rugosa Rorug02G0284600
rosa_samantha Rh2AG336800 Rh2BG344800 Rh2CG324200 Rh2DG361700
rosa_wichuraiana Rw2G027310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 13
AccI GTMKAC 2 cut(s) 93, 343
AciI CCGC 3 cut(s) 13, 104, 783
AcsI RAATTY 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 2 cut(s) 659, 749
AgsI TTSAA 4 cut(s) 25, 74, 218, 765
AjnI CCWGG 2 cut(s) 563, 658
AluBI AGCT 7 cut(s) 47, 61, 122, 214, 392, 501, 731
AluI AGCT 7 cut(s) 47, 61, 122, 214, 392, 501, 731
Alw21I GWGCWC 2 cut(s) 63, 733
Alw26I GTCTC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 246
ApeKI GCWGC 4 cut(s) 119, 300, 407, 836
ApoI RAATTY 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 1 cut(s) 601
AsuHPI GGTGA 3 cut(s) 245, 585, 663
AvaI CYCGRG 1 cut(s) 246
AvaII GGWCC 1 cut(s) 662
BanII GRGCYC 2 cut(s) 63, 733
BauI CACGAG 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 687
Bbv12I GWGCWC 2 cut(s) 63, 733
BbvCI CCTCAGC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 131, 312, 419
BccI CCATC 3 cut(s) 332, 454, 719
BceAI ACGGC 1 cut(s) 824
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 2 cut(s) 565, 660
BclI TGATCA 2 cut(s) 28, 418
BcnI CCSGG 1 cut(s) 601
BcoDI GTCTC 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 689
BisI GCNGC 4 cut(s) 120, 301, 408, 837
BlpI GCTNAGC 1 cut(s) 584
BlsI GCNGC 4 cut(s) 121, 302, 409, 838
Bme1390I CCNGG 3 cut(s) 565, 601, 660
Bme18I GGWCC 1 cut(s) 662
BmeT110I CYCGRG 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 3 cut(s) 565, 601, 660
BpiI GAAGAC 1 cut(s) 687
BpmI CTGGAG 2 cut(s) 77, 642
Bpu10I CCTNAGC 2 cut(s) 48, 367
Bpu1102I GCTNAGC 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 685
BpuMI CCSGG 1 cut(s) 601
BsaHI GRCGYC 1 cut(s) 39
Bsc4I CCNNNNNNNGG 2 cut(s) 659, 749
Bse1I ACTGG 3 cut(s) 60, 235, 278
BseBI CCWGG 2 cut(s) 565, 660
BseGI GGATG 2 cut(s) 151, 274
BseLI CCNNNNNNNGG 2 cut(s) 659, 749
BseMII CTCAG 2 cut(s) 39, 585
BseNI ACTGG 3 cut(s) 60, 235, 278
BseRI GAGGAG 2 cut(s) 341, 645
BseXI GCAGC 3 cut(s) 131, 312, 419
BsgI GTGCAG 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 63, 733
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 1 cut(s) 600
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 659, 749
BsmAI GTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 63, 733
Bsp143I GATC 2 cut(s) 28, 418
Bsp1720I GCTNAGC 1 cut(s) 584
BspACI CCGC 3 cut(s) 13, 104, 783
BspCNI CTCAG 2 cut(s) 40, 584
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 693
BsrBI CCGCTC 1 cut(s) 13
BsrI ACTGG 3 cut(s) 60, 235, 278
BssMI GATC 2 cut(s) 28, 418
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 62
Bst2BI CACGAG 1 cut(s) 62
Bst2UI CCWGG 2 cut(s) 565, 660
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 2 cut(s) 213, 450
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 4 cut(s) 48, 367, 571, 584
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 151, 274
BstKTI GATC 2 cut(s) 31, 421
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 2 cut(s) 28, 418
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 2 cut(s) 565, 660
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 563, 599, 658
BstSFI CTRYAG 1 cut(s) 689
BstV1I GCAGC 3 cut(s) 131, 312, 419
BstV2I GAAGAC 1 cut(s) 687
BtsCI GGATG 2 cut(s) 151, 274
BtsIMutI CAGTG 3 cut(s) 53, 228, 285
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 662
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 524
CviAII CATG 4 cut(s) 353, 416, 424, 578
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 4 cut(s) 48, 367, 571, 584
DpnI GATC 2 cut(s) 30, 420
DpnII GATC 2 cut(s) 28, 418
Eam1104I CTCTTC 2 cut(s) 213, 450
EarI CTCTTC 2 cut(s) 213, 450
Ecl136II GAGCTC 2 cut(s) 61, 731
Eco24I GRGCYC 2 cut(s) 63, 733
Eco47I GGWCC 1 cut(s) 662
Eco53kI GAGCTC 2 cut(s) 61, 731
Eco88I CYCGRG 1 cut(s) 246
EcoICRI GAGCTC 2 cut(s) 61, 731
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 563, 658
EcoT38I GRGCYC 2 cut(s) 63, 733
FaeI CATG 4 cut(s) 356, 419, 427, 581
FaiI YATR 8 cut(s) 127, 243, 354, 396, 417, 425, 579, 806
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 62
FatI CATG 4 cut(s) 352, 415, 423, 577
FbaI TGATCA 2 cut(s) 28, 418
FblI GTMKAC 2 cut(s) 93, 343
Fnu4HI GCNGC 4 cut(s) 120, 301, 408, 837
FokI GGATG 2 cut(s) 158, 261
FriOI GRGCYC 2 cut(s) 63, 733
Fsp4HI GCNGC 4 cut(s) 120, 301, 408, 837
GluI GCNGC 4 cut(s) 120, 301, 408, 837
GsuI CTGGAG 2 cut(s) 77, 642
HapII CCGG 1 cut(s) 600
HgaI GACGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 356, 419, 427, 581
HincII GTYRAC 1 cut(s) 344
HindII GTYRAC 1 cut(s) 344
HindIII AAGCTT 2 cut(s) 212, 390
HinfI GANTC 5 cut(s) 517, 623, 718, 746, 761
HpaII CCGG 1 cut(s) 600
HphI GGTGA 3 cut(s) 245, 585, 663
Hpy166II GTNNAC 4 cut(s) 94, 151, 236, 344
Hpy188I TCNGA 3 cut(s) 550, 574, 717
Hpy188III TCNNGA 4 cut(s) 32, 377, 722, 743
Hpy8I GTNNAC 4 cut(s) 94, 151, 236, 344
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 161, 545
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 119, 204, 415, 529, 691
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpyF3I CTNAG 4 cut(s) 48, 367, 571, 584
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 356, 419, 427, 581
Ksp22I TGATCA 2 cut(s) 28, 418
Kzo9I GATC 2 cut(s) 28, 418
LmnI GCTCC 5 cut(s) 4, 10, 58, 728, 736
Lsp1109I GCAGC 3 cut(s) 131, 312, 419
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 611, 778
MalI GATC 2 cut(s) 30, 420
MbiI CCGCTC 1 cut(s) 13
MboI GATC 2 cut(s) 28, 418
MboII GAAGA 7 cut(s) 209, 230, 235, 308, 454, 467, 687
MhlI GDGCHC 2 cut(s) 63, 733
MluCI AATT 4 cut(s) 291, 470, 530, 589
MlyI GAGTC 3 cut(s) 511, 632, 712
MseI TTAA 5 cut(s) 257, 503, 533, 629, 844
MspI CCGG 1 cut(s) 600
MspR9I CCNGG 3 cut(s) 565, 601, 660
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 2 cut(s) 565, 660
MwoI GCNNNNNNNGC 1 cut(s) 574
NciI CCSGG 1 cut(s) 601
NdeII GATC 2 cut(s) 28, 418
NlaIII CATG 4 cut(s) 356, 419, 427, 581
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 1 cut(s) 132
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 2 cut(s) 746, 761
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 4 cut(s) 121, 302, 409, 838
PleI GAGTC 3 cut(s) 511, 631, 712
PpsI GAGTC 3 cut(s) 511, 631, 712
PpuMI RGGWCCY 1 cut(s) 662
Psp124BI GAGCTC 2 cut(s) 63, 733
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 563, 658
PspGI CCWGG 2 cut(s) 563, 658
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 662
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 1 cut(s) 693
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
SacI GAGCTC 2 cut(s) 63, 733
SalI GTCGAC 1 cut(s) 342
SaqAI TTAA 5 cut(s) 257, 503, 533, 629, 844
SatI GCNGC 4 cut(s) 120, 301, 408, 837
Sau3AI GATC 2 cut(s) 28, 418
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 3 cut(s) 511, 632, 712
ScrFI CCNGG 3 cut(s) 565, 601, 660
SduI GDGCHC 2 cut(s) 63, 733
SfcI CTRYAG 1 cut(s) 689
SinI GGWCC 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SphI GCATGC 1 cut(s) 356
Sse9I AATT 4 cut(s) 291, 470, 530, 589
SsiI CCGC 3 cut(s) 13, 104, 783
SstI GAGCTC 2 cut(s) 63, 733
StyD4I CCNGG 3 cut(s) 563, 599, 658
TaaI ACNGT 1 cut(s) 240
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 343, 376, 723
TasI AATT 4 cut(s) 291, 470, 530, 589
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 2 cut(s) 746, 761
Tru1I TTAA 5 cut(s) 257, 503, 533, 629, 844
Tru9I TTAA 5 cut(s) 257, 503, 533, 629, 844
TscAI CASTG 3 cut(s) 60, 235, 285
TseI GCWGC 4 cut(s) 119, 300, 407, 836
TspDTI ATGAA 5 cut(s) 114, 153, 349, 440, 474
TspRI CASTG 3 cut(s) 60, 235, 285
VpaK11BI GGWCC 1 cut(s) 662
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 356
XmiI GTMKAC 2 cut(s) 93, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.