MD09G1239500.v1.1

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013508
Physical Location & Seq
Forward (+)
30452625 .. 30455948
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1239500.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGAGAAGGAACCGGCGGTACCGACTGAGCAAGAAGAGACGGAAGCTGAAGCTCTGGAGCTTGTTCTTTTTCAAGTGCCTGAATGTTACGTATACTTGATACCGCCGAGGAAAAGTGCCGCTTCATACAGGGCAGATGAATGGGATGTAAACAAATGGGTATGGGACGGGATATTGAAAGTAATCAGCAAAGGAGAAGAGTGCATTATCAGACTTGAAGATAAGAAAACGAATGAACTATATGCTCGGGCATTTTTGAGAAATGGAGAGCCTCATCCAGTGGAACCTGTAATTGATAGCAGCAGATACTTCGTTCTACGTGTAGAGGAGAACATTGATGGTCGGCTTCGGCATGCTTTCATTGGCATTGGATTTCGAGAAAGAACAGAAGCTTATGATTTCCAAGCCGCCTTGCATGATCACATGAAATATCTGGACAAAAAGAAAACTGCAGAAGAGATGGAACAAAAATTTCAGCAAACTTCCTTAGTTGATTACAGCTTAAAAGAGGGGGAGACTATTGTACTACAATTAAAGAACAAAACTGCCGAAATCGTGAAGTCCAAGGTTTCTGAGAACAACTCACCAGAGAAAAAGGCTAACCAAAAAGAACCCTTGATTTGTATCAAACCACCACCTCCTCCTCCGGCACCACTTTCGCCTTCTGCTACTGTTGACAAGTCTTCCTCAAACTTGCCTCCAAATCTCAATCTTGAGGGAACTTCTAAGCATGAGGCCTCGGAATCAACGGAACATGAGTCGAAGGAAACAAGCTCTTCTGGGAATCAAGGCACTCAAGATATACCAGATGACGACTTTGGGGATTTTCAGGCAGCTGGTTAA

Protein Analysis

281

Amino Acids

31.59

Weight (kDa)

4.94

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 20 - 179 8.7e-53 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58600
fragaria_vesca FvH4_6g27130
malus_domestica MD09G1239500.v1.1 MD17G1232600.v1.1
prunus_persica Prupe.2G061900_v2.0.a1 Prupe.2G061900_v2.0.a1
pyrus_communis pycom17g23530 pycom17g23550
rosa_chinensis RchiOBHm_Chr2g0128491 RchiOBHm_Chr2g0129091
rosa_laevigata RLG00000019054
rosa_roxburghii Rroxscaffold_2G00114880
rosa_rugosa Rorug02G0284600
rosa_samantha Rh2AG336800 Rh2BG344800 Rh2CG324200 Rh2DG361700
rosa_wichuraiana Rw2G027310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 2 cut(s) 19, 649
AccI GTMKAC 1 cut(s) 93
AciI CCGC 4 cut(s) 17, 104, 120, 408
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 2 cut(s) 21, 525
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 74, 178, 218
AluBI AGCT 7 cut(s) 47, 53, 61, 392, 501, 774, 836
AluI AGCT 7 cut(s) 47, 53, 61, 392, 501, 774, 836
Alw26I GTCTC 2 cut(s) 32, 509
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 1 cut(s) 735
ApeKI GCWGC 2 cut(s) 300, 833
ApoI RAATTY 1 cut(s) 470
Asp718I GGTACC 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 576
AvaI CYCGRG 1 cut(s) 246
BanI GGYRCC 2 cut(s) 19, 649
BbsI GAAGAC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 332, 454
BcgI CGANNNNNNTGC 4 cut(s) 292, 326, 639, 673
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 32, 509
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 4 cut(s) 120, 301, 408, 834
BlsI GCNGC 4 cut(s) 121, 302, 409, 835
BmeT110I CYCGRG 1 cut(s) 246
BmiI GGNNCC 4 cut(s) 12, 21, 285, 651
BpiI GAAGAC 1 cut(s) 675
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 2 cut(s) 734, 780
BsaAI YACGTR 2 cut(s) 91, 320
BsaBI GATNNNNATC 1 cut(s) 623
BsaJI CCNNGG 3 cut(s) 107, 564, 738
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 278
Bse8I GATNNNNATC 1 cut(s) 623
BseDI CCNNGG 3 cut(s) 107, 564, 738
BseGI GGATG 2 cut(s) 151, 274
BseJI GATNNNNATC 1 cut(s) 623
BseMII CTCAG 2 cut(s) 18, 564
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 3 cut(s) 341, 630, 633
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 1 cut(s) 737
BshNI GGYRCC 2 cut(s) 19, 649
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 2 cut(s) 14, 647
BslFI GGGAC 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 32, 509
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 1 cut(s) 737
BsoBI CYCGRG 1 cut(s) 246
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 4 cut(s) 17, 104, 120, 408
BspANI GGCC 1 cut(s) 737
BspCNI CTCAG 2 cut(s) 19, 565
BspLI GGNNCC 4 cut(s) 12, 21, 285, 651
BspMAI CTGCAG 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 781
BspT107I GGYRCC 2 cut(s) 19, 649
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 3 cut(s) 107, 564, 738
BssMI GATC 1 cut(s) 418
BssNAI GTATAC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 564
Bst1107I GTATAC 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 673
Bst6I CTCTTC 4 cut(s) 30, 192, 450, 781
BstBAI YACGTR 2 cut(s) 91, 320
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 4 cut(s) 27, 487, 573, 726
BstF5I GGATG 2 cut(s) 151, 274
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 2 cut(s) 32, 509
BstMBI GATC 1 cut(s) 418
BstNSI RCATGY 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 450
BstSNI TACGTA 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 675
BstZ17I GTATAC 1 cut(s) 94
BsuRI GGCC 1 cut(s) 737
BtsCI GGATG 2 cut(s) 151, 274
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 13
Csp6I GTAC 2 cut(s) 20, 524
CviAII CATG 5 cut(s) 353, 416, 424, 731, 755
CviQI GTAC 2 cut(s) 20, 524
DdeI CTNAG 4 cut(s) 27, 487, 573, 726
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 4 cut(s) 30, 192, 450, 781
EarI CTCTTC 4 cut(s) 30, 192, 450, 781
Eco105I TACGTA 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 564
Eco147I AGGCCT 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 5 cut(s) 356, 419, 427, 734, 758
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 179
FatI CATG 5 cut(s) 352, 415, 423, 730, 754
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 4 cut(s) 120, 301, 408, 834
FokI GGATG 2 cut(s) 158, 261
Fsp4HI GCNGC 4 cut(s) 120, 301, 408, 834
GluI GCNGC 4 cut(s) 120, 301, 408, 834
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 737
HapII CCGG 2 cut(s) 14, 647
Hin1II CATG 5 cut(s) 356, 419, 427, 734, 758
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 3 cut(s) 743, 758, 784
HpaII CCGG 2 cut(s) 14, 647
HphI GGTGA 1 cut(s) 576
Hpy166II GTNNAC 3 cut(s) 94, 151, 676
Hpy188I TCNGA 3 cut(s) 212, 574, 742
Hpy188III TCNNGA 6 cut(s) 56, 377, 434, 556, 713, 797
Hpy8I GTNNAC 3 cut(s) 94, 151, 676
HpyAV CCTTC 2 cut(s) 672, 757
HpyCH4III ACNGT 1 cut(s) 673
HpyCH4IV ACGT 2 cut(s) 90, 319
HpyCH4V TGCA 3 cut(s) 204, 415, 452
HpyF3I CTNAG 4 cut(s) 27, 487, 573, 726
HpySE526I ACGT 2 cut(s) 90, 319
Hsp92II CATG 5 cut(s) 356, 419, 427, 734, 758
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 1 cut(s) 418
LguI GCTCTTC 1 cut(s) 781
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 1 cut(s) 312
MaeII ACGT 2 cut(s) 90, 319
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 6 cut(s) 47, 209, 230, 467, 675, 768
MluCI AATT 3 cut(s) 291, 470, 530
MlyI GAGTC 1 cut(s) 767
MseI TTAA 3 cut(s) 503, 533, 841
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 2 cut(s) 14, 647
NdeII GATC 1 cut(s) 418
NlaIII CATG 5 cut(s) 356, 419, 427, 734, 758
NlaIV GGNNCC 4 cut(s) 12, 21, 285, 651
NmeAIII GCCGAG 1 cut(s) 132
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PceI AGGCCT 1 cut(s) 737
PciSI GCTCTTC 1 cut(s) 781
PfeI GAWTC 2 cut(s) 743, 784
PkrI GCNGC 4 cut(s) 121, 302, 409, 835
PleI GAGTC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 766
Ppu21I YACGTR 2 cut(s) 91, 320
PspN4I GGNNCC 4 cut(s) 12, 21, 285, 651
PsrI GAACNNNNNNTAC 1 cut(s) 35
PstI CTGCAG 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 2 cut(s) 21, 525
RsaNI GTAC 2 cut(s) 20, 524
SapI GCTCTTC 1 cut(s) 781
SaqAI TTAA 3 cut(s) 503, 533, 841
SatI GCNGC 4 cut(s) 120, 301, 408, 834
Sau3AI GATC 1 cut(s) 418
SchI GAGTC 1 cut(s) 767
SfcI CTRYAG 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 713, 795
SmoI CTYRAG 2 cut(s) 713, 795
SnaBI TACGTA 1 cut(s) 91
SphI GCATGC 1 cut(s) 356
Sse9I AATT 3 cut(s) 291, 470, 530
SseBI AGGCCT 1 cut(s) 737
SsiI CCGC 4 cut(s) 17, 104, 120, 408
StuI AGGCCT 1 cut(s) 737
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 1 cut(s) 673
TaiI ACGT 2 cut(s) 93, 322
TaqI TCGA 2 cut(s) 376, 761
TasI AATT 3 cut(s) 291, 470, 530
TatI WGTACW 1 cut(s) 523
TauI GCSGC 2 cut(s) 122, 410
TfiI GAWTC 2 cut(s) 743, 784
Tru1I TTAA 3 cut(s) 503, 533, 841
Tru9I TTAA 3 cut(s) 503, 533, 841
TscAI CASTG 1 cut(s) 285
TseI GCWGC 2 cut(s) 300, 833
TspDTI ATGAA 5 cut(s) 114, 153, 249, 349, 440
TspGWI ACGGA 2 cut(s) 56, 764
TspRI CASTG 1 cut(s) 285
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 356
XmiI GTMKAC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.