Rh2AG336800

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013508
Physical Location & Seq
Reverse (-)
48841171 .. 48844913
3743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG336800.1

Sequence Viewer

Length: 597 bp
ATGGAGCAGGATCAGAAGATATTGAATGATCAAGACGACGCCGAAGTTGAGGCACTGGAGCTTGTGCTTTTCCAAGTCTCTGAATGCTACGTCTACTTGATACCACCGAGAAAAAGTGCAGCTTCGTACAGGGCTGATGAATGGGATGTAAACAAATGGGTTTGGGAAGGAATATTGAAAGTCATTAGCAAAGGAGAAGATTGCATTATCAGACTTGAAGAGAAGAACACTGGTGAACTATATGCTCGGGCATTTTTACGAAAAGGAGAGCCGCATCCAGTTGAACCTGTAATTGATAGCAGCAGATACTTTGTTCTTCGTGTGGAGGAGAATATTGATGGTCGACTTCGGCATGCTTTCATTGGCCTAGGATTTCGAGAAAGAACAGAAGCTTATGATTTCCAAGCAGCCCTGCATGATCACATGAAATATCTAAACAAGAAGAAAACTGCGGAAGAGATGGAACAGAAATTTCAGCAAACTTCATCAGTTGATTACAGCTTAAAAGAGGGGGAGACTCTTGTACTGCAGTTAAAAAGTGTTAGTGCATCTGTATTTTATTGTCAACTCTGTCTTCTCATTCATGCTACTTACTAG

Protein Analysis

198

Amino Acids

23.05

Weight (kDa)

5.14

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 20 - 179 4.2e-53 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58600
fragaria_vesca FvH4_6g27130
malus_domestica MD09G1239500.v1.1 MD17G1232600.v1.1
prunus_persica Prupe.2G061900_v2.0.a1 Prupe.2G061900_v2.0.a1
pyrus_communis pycom17g23530 pycom17g23550
rosa_chinensis RchiOBHm_Chr2g0128491 RchiOBHm_Chr2g0129091
rosa_laevigata RLG00000019054
rosa_roxburghii Rroxscaffold_2G00114880
rosa_rugosa Rorug02G0284600
rosa_samantha Rh2AG336800 Rh2BG344800 Rh2CG324200 Rh2DG361700
rosa_wichuraiana Rw2G027310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 93, 343
AciI CCGC 2 cut(s) 272, 452
AclWI GGATC 1 cut(s) 18
AcsI RAATTY 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 128, 525
AgsI TTSAA 4 cut(s) 25, 178, 218, 284
AluBI AGCT 4 cut(s) 61, 122, 392, 501
AluI AGCT 4 cut(s) 61, 122, 392, 501
Alw26I GTCTC 2 cut(s) 82, 509
AlwI GGATC 1 cut(s) 18
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 119, 300, 407
ApoI RAATTY 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 246
AvrII CCTAGG 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 566
BbvI GCAGC 3 cut(s) 131, 312, 419
BccI CCATC 2 cut(s) 332, 454
BclI TGATCA 2 cut(s) 28, 418
BcoDI GTCTC 2 cut(s) 82, 509
BfaI CTAG 2 cut(s) 368, 595
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 4 cut(s) 120, 272, 301, 408
BlnI CCTAGG 1 cut(s) 367
BlsI GCNGC 4 cut(s) 121, 273, 302, 409
BmeT110I CYCGRG 1 cut(s) 246
BmsI GCATC 2 cut(s) 283, 557
BpiI GAAGAC 1 cut(s) 566
BpmI CTGGAG 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 367
Bse1I ACTGG 3 cut(s) 60, 235, 278
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 151, 274
BseNI ACTGG 3 cut(s) 60, 235, 278
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 131, 312, 419
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 246
BsmAI GTCTC 2 cut(s) 82, 509
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 246
Bsp143I GATC 3 cut(s) 10, 28, 418
BspACI CCGC 2 cut(s) 272, 452
BspANI GGCC 1 cut(s) 366
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 1 cut(s) 18
BsrI ACTGG 3 cut(s) 60, 235, 278
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 3 cut(s) 10, 28, 418
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 367
Bst6I CTCTTC 2 cut(s) 213, 450
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 2 cut(s) 151, 274
BstKTI GATC 3 cut(s) 13, 31, 421
BstMAI GTCTC 2 cut(s) 82, 509
BstMBI GATC 3 cut(s) 10, 28, 418
BstNSI RCATGY 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 527
BstV1I GCAGC 3 cut(s) 131, 312, 419
BstV2I GAAGAC 1 cut(s) 566
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 2 cut(s) 151, 274
BtsIMutI CAGTG 2 cut(s) 53, 228
Cac8I GCNNGC 1 cut(s) 354
CseI GACGC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 127, 524
CviAII CATG 4 cut(s) 353, 416, 424, 584
CviJI RGCY 8 cut(s) 61, 122, 134, 271, 366, 392, 410, 501
CviKI_1 RGCY 8 cut(s) 61, 122, 134, 271, 366, 392, 410, 501
CviQI GTAC 2 cut(s) 127, 524
DpnI GATC 3 cut(s) 12, 30, 420
DpnII GATC 3 cut(s) 10, 28, 418
Eam1104I CTCTTC 2 cut(s) 213, 450
EarI CTCTTC 2 cut(s) 213, 450
Eco130I CCWWGG 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 4 cut(s) 356, 419, 427, 587
FaiI YATR 7 cut(s) 241, 243, 354, 396, 417, 425, 585
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FatI CATG 4 cut(s) 352, 415, 423, 583
FbaI TGATCA 2 cut(s) 28, 418
FblI GTMKAC 2 cut(s) 93, 343
Fnu4HI GCNGC 4 cut(s) 120, 272, 301, 408
FokI GGATG 2 cut(s) 158, 261
Fsp4HI GCNGC 4 cut(s) 120, 272, 301, 408
FspBI CTAG 2 cut(s) 368, 595
GluI GCNGC 4 cut(s) 120, 272, 301, 408
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 366
HgaI GACGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 356, 419, 427, 587
HincII GTYRAC 2 cut(s) 344, 566
HindII GTYRAC 2 cut(s) 344, 566
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 1 cut(s) 517
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 5 cut(s) 94, 151, 236, 344, 566
Hpy188I TCNGA 3 cut(s) 15, 82, 212
Hpy188III TCNNGA 2 cut(s) 32, 377
Hpy8I GTNNAC 5 cut(s) 94, 151, 236, 344, 566
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 119, 204, 415, 529, 548
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 356, 419, 427, 587
Ksp22I TGATCA 2 cut(s) 28, 418
Kzo9I GATC 3 cut(s) 10, 28, 418
LmnI GCTCC 2 cut(s) 4, 58
LpnPI CCDG 6 cut(s) 41, 115, 216, 291, 300, 425
Lsp1109I GCAGC 3 cut(s) 131, 312, 419
LweI GCATC 2 cut(s) 283, 557
MaeI CTAG 2 cut(s) 368, 595
MaeII ACGT 1 cut(s) 90
MalI GATC 3 cut(s) 12, 30, 420
MboI GATC 3 cut(s) 10, 28, 418
MboII GAAGA 8 cut(s) 28, 209, 230, 235, 308, 454, 467, 566
MluCI AATT 2 cut(s) 291, 470
MlyI GAGTC 1 cut(s) 511
MnlI CCTC 3 cut(s) 43, 319, 502
MseI TTAA 2 cut(s) 503, 533
Mva1269I GAATGC 1 cut(s) 89
NdeII GATC 3 cut(s) 10, 28, 418
NlaIII CATG 4 cut(s) 356, 419, 427, 587
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PctI GAATGC 1 cut(s) 89
PkrI GCNGC 4 cut(s) 121, 273, 302, 409
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PstI CTGCAG 1 cut(s) 531
RsaI GTAC 2 cut(s) 128, 525
RsaNI GTAC 2 cut(s) 127, 524
SalI GTCGAC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 503, 533
SatI GCNGC 4 cut(s) 120, 272, 301, 408
Sau3AI GATC 3 cut(s) 10, 28, 418
SchI GAGTC 1 cut(s) 511
SetI ASST 6 cut(s) 63, 93, 124, 289, 394, 503
SfaNI GCATC 2 cut(s) 283, 557
SfcI CTRYAG 1 cut(s) 527
SphI GCATGC 1 cut(s) 356
Sse9I AATT 2 cut(s) 291, 470
SsiI CCGC 2 cut(s) 272, 452
SspI AATATT 2 cut(s) 174, 334
SspMI CTAG 2 cut(s) 368, 595
StyI CCWWGG 1 cut(s) 367
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 343, 376
TasI AATT 2 cut(s) 291, 470
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 503, 533
Tru9I TTAA 2 cut(s) 503, 533
TscAI CASTG 2 cut(s) 60, 235
TseI GCWGC 3 cut(s) 119, 300, 407
TspDTI ATGAA 5 cut(s) 153, 349, 440, 474, 572
TspRI CASTG 2 cut(s) 60, 235
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 356
XmaJI CCTAGG 1 cut(s) 367
XmiI GTMKAC 2 cut(s) 93, 343
XspI CTAG 2 cut(s) 368, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.