Rw2G027310

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013508
Physical Location & Seq
Reverse (-)
42714827 .. 42718275
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027310.1

Sequence Viewer

Length: 846 bp
ATGGAGCAGGATCAGAAGATATTGAATGATCAAGACGACGCCGAAGTTGAGGCACTGGAGCTTGTGCTTTTCCAAGTCTCTGAATGCTACGTCTACTTGATACCACCCAGAAAAAGTGCAGCTTCGTACAGGGCTGATGAATGGGATGTAAACAAATGGGTTTGGGAAGGAATATTGAAAGTCATTAGCAAAGGAGAAGATTGCATTATCAGACTTGAAGAGAAGAACACTGGTGAACTATATGCTCGGGCATTTTTACGAAAAGGAGAGCCGCATCCAGTTGAACCTGTAATTGATAGCAGCAGATACTTTGTTCTTCGTGTAGAGGAGAATATTGATGGTCGACTTCGGCATGCTTTCATTGGCCTAGGATTTCGAGAAAGAACAGAAGCTTATGATTTCCAAGCAGCCCTGCATGATCACATGAAATATCTAAACAAGAAGAAAACTGCGGAAGAGATGGAACAGAAATTTCAGCAAACTTCATCAGTTGATTACAGCTTAAAAGAGGGGGAGACTCTTGTACTGCAGTTAAAAAGTAAAAGTTCTGAAGGTGTGAAGTCCAAGCTCTCAGAGCAGGGAGTGAGCAATTCGCCACCCGCGAAAAAGAGTAACCAAAAAGAGTCCTTGATCTGTATCAAACCACCACCACCTCCTCCAGGACCACTCTCTCCTTCTACGAATGTCCAGAAGTCTCCCTCAAACCCACCAAATCTGGCTCTCGAGGGAGCTTCTAAACTTCCAGAATCAGTGGCAGATGATTCCAAAGAAATGAATACTTCTGCAAATCAAAGCACGCAAGAAATACCAGATGATGACTTTGGGGATTTTCAGGCTGCTGTTTAA

Protein Analysis

281

Amino Acids

31.54

Weight (kDa)

4.99

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 20 - 179 1.2e-52 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58600
fragaria_vesca FvH4_6g27130
malus_domestica MD09G1239500.v1.1 MD17G1232600.v1.1
prunus_persica Prupe.2G061900_v2.0.a1 Prupe.2G061900_v2.0.a1
pyrus_communis pycom17g23530 pycom17g23550
rosa_chinensis RchiOBHm_Chr2g0128491 RchiOBHm_Chr2g0129091
rosa_laevigata RLG00000019054
rosa_roxburghii Rroxscaffold_2G00114880
rosa_rugosa Rorug02G0284600
rosa_samantha Rh2AG336800 Rh2BG344800 Rh2CG324200 Rh2DG361700
rosa_wichuraiana Rw2G027310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 93, 343
AccII CGCG 1 cut(s) 602
AciI CCGC 3 cut(s) 272, 452, 600
AclWI GGATC 1 cut(s) 18
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 570
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 128, 525
AfiI CCNNNNNNNGG 1 cut(s) 659
AgsI TTSAA 4 cut(s) 25, 178, 218, 284
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 6 cut(s) 61, 122, 392, 501, 568, 731
AluI AGCT 6 cut(s) 61, 122, 392, 501, 568, 731
Alw26I GTCTC 3 cut(s) 82, 509, 699
AlwI GGATC 1 cut(s) 18
Ama87I CYCGRG 2 cut(s) 246, 722
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 4 cut(s) 119, 300, 407, 836
ApoI RAATTY 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 662
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 2 cut(s) 246, 722
AvaII GGWCC 1 cut(s) 662
AvrII CCTAGG 1 cut(s) 367
BbvI GCAGC 4 cut(s) 131, 312, 419, 823
BccI CCATC 2 cut(s) 332, 454
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 2 cut(s) 28, 418
BcoDI GTCTC 3 cut(s) 82, 509, 699
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 5 cut(s) 120, 272, 301, 408, 837
BlnI CCTAGG 1 cut(s) 367
BlsI GCNGC 5 cut(s) 121, 273, 302, 409, 838
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 1 cut(s) 662
BmeT110I CYCGRG 2 cut(s) 246, 722
BmgT120I GGNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 283
BpmI CTGGAG 2 cut(s) 77, 642
BsaBI GATNNNNATC 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 1 cut(s) 659
Bse1I ACTGG 3 cut(s) 60, 235, 278
Bse8I GATNNNNATC 1 cut(s) 635
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 151, 274
BseJI GATNNNNATC 1 cut(s) 635
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 3 cut(s) 60, 235, 278
BseRI GAGGAG 2 cut(s) 341, 645
BseXI GCAGC 4 cut(s) 131, 312, 419, 823
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 246, 722
BslI CCNNNNNNNGG 1 cut(s) 659
BsmAI GTCTC 3 cut(s) 82, 509, 699
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 2 cut(s) 246, 722
Bsp143I GATC 4 cut(s) 10, 28, 418, 630
BspACI CCGC 3 cut(s) 272, 452, 600
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 1 cut(s) 584
BspFNI CGCG 1 cut(s) 602
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 1 cut(s) 18
BsrI ACTGG 3 cut(s) 60, 235, 278
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 4 cut(s) 10, 28, 418, 630
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 660
Bst6I CTCTTC 2 cut(s) 213, 450
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 354, 797
BstDEI CTNAG 1 cut(s) 571
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 151, 274
BstFNI CGCG 1 cut(s) 602
BstKTI GATC 4 cut(s) 13, 31, 421, 633
BstMAI GTCTC 3 cut(s) 82, 509, 699
BstMBI GATC 4 cut(s) 10, 28, 418, 630
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 658
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 4 cut(s) 131, 312, 419, 823
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 2 cut(s) 151, 274
BtsIMutI CAGTG 3 cut(s) 53, 228, 756
Cac8I GCNNGC 2 cut(s) 354, 797
Cfr13I GGNCC 1 cut(s) 662
CseI GACGC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 127, 524
CviAII CATG 3 cut(s) 353, 416, 424
CviQI GTAC 2 cut(s) 127, 524
DdeI CTNAG 1 cut(s) 571
DpnI GATC 4 cut(s) 12, 30, 420, 632
DpnII GATC 4 cut(s) 10, 28, 418, 630
Eam1104I CTCTTC 2 cut(s) 213, 450
EarI CTCTTC 2 cut(s) 213, 450
Eco130I CCWWGG 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 570
Eco88I CYCGRG 2 cut(s) 246, 722
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 3 cut(s) 356, 419, 427
FaiI YATR 6 cut(s) 241, 243, 354, 396, 417, 425
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FatI CATG 3 cut(s) 352, 415, 423
FauI CCCGC 1 cut(s) 607
FbaI TGATCA 2 cut(s) 28, 418
FblI GTMKAC 2 cut(s) 93, 343
Fnu4HI GCNGC 5 cut(s) 120, 272, 301, 408, 837
FokI GGATG 2 cut(s) 158, 261
Fsp4HI GCNGC 5 cut(s) 120, 272, 301, 408, 837
FspBI CTAG 1 cut(s) 368
GluI GCNGC 5 cut(s) 120, 272, 301, 408, 837
GsuI CTGGAG 2 cut(s) 77, 642
HaeIII GGCC 1 cut(s) 366
HgaI GACGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 3 cut(s) 356, 419, 427
HincII GTYRAC 1 cut(s) 344
HindII GTYRAC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 4 cut(s) 517, 623, 746, 761
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 4 cut(s) 94, 151, 236, 344
Hpy188I TCNGA 5 cut(s) 15, 82, 212, 550, 574
Hpy188III TCNNGA 5 cut(s) 32, 377, 688, 722, 743
Hpy8I GTNNAC 4 cut(s) 94, 151, 236, 344
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 161, 545, 684
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 119, 204, 415, 529, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpyF3I CTNAG 1 cut(s) 571
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 3 cut(s) 356, 419, 427
Ksp22I TGATCA 2 cut(s) 28, 418
Kzo9I GATC 4 cut(s) 10, 28, 418, 630
LmnI GCTCC 3 cut(s) 4, 58, 728
Lsp1109I GCAGC 4 cut(s) 131, 312, 419, 823
LweI GCATC 1 cut(s) 283
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 611
MalI GATC 4 cut(s) 12, 30, 420, 632
MboI GATC 4 cut(s) 10, 28, 418, 630
MboII GAAGA 7 cut(s) 28, 209, 230, 235, 308, 454, 467
MluCI AATT 3 cut(s) 291, 470, 589
MlyI GAGTC 2 cut(s) 511, 632
MnlI CCTC 7 cut(s) 43, 319, 502, 663, 666, 709, 718
MseI TTAA 3 cut(s) 503, 533, 844
MspR9I CCNGG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 574
NdeII GATC 4 cut(s) 10, 28, 418, 630
NlaIII CATG 3 cut(s) 356, 419, 427
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 722
PcsI WCGNNNNNNNCGW 1 cut(s) 599
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 2 cut(s) 746, 761
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 5 cut(s) 121, 273, 302, 409, 838
PleI GAGTC 2 cut(s) 511, 631
PpsI GAGTC 2 cut(s) 511, 631
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspPI GGNCC 1 cut(s) 662
PstI CTGCAG 1 cut(s) 531
RsaI GTAC 2 cut(s) 128, 525
RsaNI GTAC 2 cut(s) 127, 524
SalI GTCGAC 1 cut(s) 342
SaqAI TTAA 3 cut(s) 503, 533, 844
SatI GCNGC 5 cut(s) 120, 272, 301, 408, 837
Sau3AI GATC 4 cut(s) 10, 28, 418, 630
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 2 cut(s) 511, 632
ScrFI CCNGG 1 cut(s) 660
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 1 cut(s) 527
Sfr274I CTCGAG 1 cut(s) 722
SinI GGWCC 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SphI GCATGC 1 cut(s) 356
Sse9I AATT 3 cut(s) 291, 470, 589
SsiI CCGC 3 cut(s) 272, 452, 600
SspI AATATT 2 cut(s) 174, 334
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 1 cut(s) 367
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 343, 376, 723
TasI AATT 3 cut(s) 291, 470, 589
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 2 cut(s) 746, 761
Tru1I TTAA 3 cut(s) 503, 533, 844
Tru9I TTAA 3 cut(s) 503, 533, 844
TscAI CASTG 3 cut(s) 60, 235, 756
TseI GCWGC 4 cut(s) 119, 300, 407, 836
TspDTI ATGAA 5 cut(s) 153, 349, 440, 474, 788
TspRI CASTG 3 cut(s) 60, 235, 756
VpaK11BI GGWCC 1 cut(s) 662
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 722
XmaJI CCTAGG 1 cut(s) 367
XmiI GTMKAC 2 cut(s) 93, 343
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.