MD17G1232600.v1.1

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013508
Physical Location & Seq
Forward (+)
28090752 .. 28094054
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1232600.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGAGAAGGAACCGGTGGTACCGAACCAGCAAGAAGAAACGGAAGCTGAAGCTTTGGAGCTTGTTCTTTTTCAAGTGCCTGAATGTTACGTGTACTTGATACCGCCGAGGAAAAGTGCAGCTTCATACAGGGCTGATGAATGGGATGTAAACAAATGGGTATGGGAAGGGATATTGAAAGTAATCAGCAAAGGAGAAGAGTGCATTATCAGACTTGAAGATAAGAACACCAGTGAACTATATGCTCGGGCGTTTTTGAGAAATGGAGAGCCTTATCCGGTGGAACCTGTAATTGATAGCAGCAGATATTTTGTTCTACGTGTAGAGGAGAACATTGATGGTCAACTTCGGCATGCATTCATTGGCCTTGGATTTCGAGAAGGAACAGAAGCTTATGATTTCCAAGCCGCCTTGCATGATCACATGAAATATCTGGACAAAAAGAAAACTGCTGAAGAGATGGAACAAAAATTTCAGCAAACTTCCTCAGTTGATTACAGCTTAAAAGAGGGGGAGACTATTGTCCTACAATTAAAGAATAAAACTGCCGAAATCGTGAAGTCCAAGGTTTCTGAGAACAACTCCTCACCAGAGAAAAAGGCTAACAAAAAAGAACCCTTGATTTGTATCAAACCACCACCTCCTCCCCCGGCACCACTTTCGCCTTCTGCTACTGTTGACAAGTCTTCCTCAAACTTGCCTTCAAATCTCAACCTTGAGGGAACTTCTAAGCACGAGGCCTCAGAATCACCGGAACATGAGTCGAAAGAAACAAGCTCTTCCGGGAATCAAAGCACTCAAGATATACCAGATGACGACTTTGGGGATTTTCAGACAGCCGTTTAA

Protein Analysis

282

Amino Acids

31.65

Weight (kDa)

4.84

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 20 - 179 2.9e-51 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58600
fragaria_vesca FvH4_6g27130
malus_domestica MD09G1239500.v1.1 MD17G1232600.v1.1
prunus_persica Prupe.2G061900_v2.0.a1 Prupe.2G061900_v2.0.a1
pyrus_communis pycom17g23530 pycom17g23550
rosa_chinensis RchiOBHm_Chr2g0128491 RchiOBHm_Chr2g0129091
rosa_laevigata RLG00000019054
rosa_roxburghii Rroxscaffold_2G00114880
rosa_rugosa Rorug02G0284600
rosa_samantha Rh2AG336800 Rh2BG344800 Rh2CG324200 Rh2DG361700
rosa_wichuraiana Rw2G027310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 2 cut(s) 19, 652
AciI CCGC 2 cut(s) 104, 408
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 2 cut(s) 69, 474
AfaI GTAC 2 cut(s) 21, 95
AflIII ACRYGT 2 cut(s) 90, 319
AgeI ACCGGT 1 cut(s) 13
AgsI TTSAA 4 cut(s) 74, 178, 218, 705
AluBI AGCT 7 cut(s) 47, 53, 61, 122, 392, 501, 777
AluI AGCT 7 cut(s) 47, 53, 61, 122, 392, 501, 777
Alw26I GTCTC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 2 cut(s) 364, 738
ApeKI GCWGC 2 cut(s) 119, 300
ApoI RAATTY 1 cut(s) 470
AsiGI ACCGGT 1 cut(s) 13
Asp718I GGTACC 1 cut(s) 19
AsuC2I CCSGG 2 cut(s) 650, 784
AsuHPI GGTGA 2 cut(s) 579, 741
AvaI CYCGRG 1 cut(s) 246
BanI GGYRCC 2 cut(s) 19, 652
BauI CACGAG 1 cut(s) 734
BbsI GAAGAC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 131, 312
BccI CCATC 2 cut(s) 332, 454
BceAI ACGGC 1 cut(s) 824
BcgI CGANNNNNNTGC 2 cut(s) 642, 676
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 2 cut(s) 650, 784
BcoDI GTCTC 1 cut(s) 509
BisI GCNGC 3 cut(s) 120, 301, 408
BlsI GCNGC 3 cut(s) 121, 302, 409
Bme1390I CCNGG 2 cut(s) 650, 784
BmeT110I CYCGRG 1 cut(s) 246
BmiI GGNNCC 4 cut(s) 12, 21, 285, 654
BmrFI CCNGG 2 cut(s) 650, 784
BoxI GACNNNNGTC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpuEI CTTGAG 2 cut(s) 737, 783
BpuMI CCSGG 2 cut(s) 650, 784
BsaAI YACGTR 2 cut(s) 91, 320
BsaBI GATNNNNATC 1 cut(s) 626
BsaJI CCNNGG 4 cut(s) 107, 367, 564, 648
BsaWI WCCGGW 3 cut(s) 13, 277, 751
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 626
BseDI CCNNGG 4 cut(s) 107, 367, 564, 648
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 626
BseMII CTCAG 3 cut(s) 501, 564, 756
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 3 cut(s) 341, 574, 633
BseXI GCAGC 2 cut(s) 131, 312
BsgI GTGCAG 1 cut(s) 138
BshFI GGCC 2 cut(s) 366, 740
BshNI GGYRCC 2 cut(s) 19, 652
BshTI ACCGGT 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 5 cut(s) 14, 278, 650, 752, 783
BsmAI GTCTC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 2 cut(s) 366, 740
BsoBI CYCGRG 1 cut(s) 246
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 2 cut(s) 104, 408
BspANI GGCC 2 cut(s) 366, 740
BspCNI CTCAG 3 cut(s) 500, 565, 755
BspLI GGNNCC 4 cut(s) 12, 21, 285, 654
BspQI GCTCTTC 1 cut(s) 784
BspT107I GGYRCC 2 cut(s) 19, 652
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 4 cut(s) 107, 367, 564, 648
BssMI GATC 1 cut(s) 418
BssSI CACGAG 1 cut(s) 734
BssT1I CCWWGG 2 cut(s) 367, 564
Bst2BI CACGAG 1 cut(s) 734
Bst4CI ACNGT 1 cut(s) 676
Bst6I CTCTTC 3 cut(s) 192, 450, 784
BstBAI YACGTR 2 cut(s) 91, 320
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 4 cut(s) 487, 573, 729, 742
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 1 cut(s) 418
BstNSI RCATGY 1 cut(s) 356
BstPAI GACNNNNGTC 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 648, 782
BstV1I GCAGC 2 cut(s) 131, 312
BstV2I GAAGAC 1 cut(s) 678
BsuRI GGCC 2 cut(s) 366, 740
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 13
Csp6I GTAC 2 cut(s) 20, 94
CspAI ACCGGT 1 cut(s) 13
CviAII CATG 4 cut(s) 353, 416, 424, 758
CviQI GTAC 2 cut(s) 20, 94
DdeI CTNAG 4 cut(s) 487, 573, 729, 742
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 3 cut(s) 192, 450, 784
EarI CTCTTC 3 cut(s) 192, 450, 784
Eco130I CCWWGG 2 cut(s) 367, 564
Eco147I AGGCCT 1 cut(s) 740
Eco57I CTGAAG 2 cut(s) 69, 474
Eco88I CYCGRG 1 cut(s) 246
EcoT14I CCWWGG 2 cut(s) 367, 564
EcoT22I ATGCAT 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 367, 564
FaeI CATG 4 cut(s) 356, 419, 427, 761
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FatI CATG 4 cut(s) 352, 415, 423, 757
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 3 cut(s) 120, 301, 408
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 3 cut(s) 120, 301, 408
GluI GCNGC 3 cut(s) 120, 301, 408
HaeIII GGCC 2 cut(s) 366, 740
HapII CCGG 5 cut(s) 14, 278, 650, 752, 783
Hin1II CATG 4 cut(s) 356, 419, 427, 761
HincII GTYRAC 2 cut(s) 344, 679
HindII GTYRAC 2 cut(s) 344, 679
HindIII AAGCTT 2 cut(s) 51, 390
HinfI GANTC 3 cut(s) 746, 761, 787
HpaII CCGG 5 cut(s) 14, 278, 650, 752, 783
HphI GGTGA 2 cut(s) 579, 741
Hpy166II GTNNAC 5 cut(s) 94, 151, 236, 344, 679
Hpy188I TCNGA 4 cut(s) 212, 574, 745, 834
Hpy188III TCNNGA 4 cut(s) 377, 434, 556, 800
Hpy8I GTNNAC 5 cut(s) 94, 151, 236, 344, 679
HpyAV CCTTC 4 cut(s) 161, 374, 675, 711
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 2 cut(s) 90, 319
HpyCH4V TGCA 4 cut(s) 119, 204, 356, 415
HpyF3I CTNAG 4 cut(s) 487, 573, 729, 742
HpySE526I ACGT 2 cut(s) 90, 319
Hsp92II CATG 4 cut(s) 356, 419, 427, 761
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 1 cut(s) 418
LguI GCTCTTC 1 cut(s) 784
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 131, 312
MaeII ACGT 2 cut(s) 90, 319
MaeIII GTNAC 1 cut(s) 86
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 6 cut(s) 47, 209, 230, 467, 678, 771
MluCI AATT 3 cut(s) 291, 470, 530
MlyI GAGTC 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 358
MseI TTAA 3 cut(s) 503, 533, 844
MspI CCGG 5 cut(s) 14, 278, 650, 752, 783
MspR9I CCNGG 2 cut(s) 650, 784
Mva1269I GAATGC 1 cut(s) 356
NciI CCSGG 2 cut(s) 650, 784
NdeII GATC 1 cut(s) 418
NlaIII CATG 4 cut(s) 356, 419, 427, 761
NlaIV GGNNCC 4 cut(s) 12, 21, 285, 654
NmeAIII GCCGAG 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 358
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PceI AGGCCT 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 784
PctI GAATGC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 746, 787
PfoI TCCNGGA 1 cut(s) 782
PinAI ACCGGT 1 cut(s) 13
PkrI GCNGC 3 cut(s) 121, 302, 409
PleI GAGTC 1 cut(s) 769
PpsI GAGTC 1 cut(s) 769
Ppu21I YACGTR 2 cut(s) 91, 320
PshAI GACNNNNGTC 1 cut(s) 521
PspN4I GGNNCC 4 cut(s) 12, 21, 285, 654
PsrI GAACNNNNNNTAC 1 cut(s) 35
RsaI GTAC 2 cut(s) 21, 95
RsaNI GTAC 2 cut(s) 20, 94
SapI GCTCTTC 1 cut(s) 784
SaqAI TTAA 3 cut(s) 503, 533, 844
SatI GCNGC 3 cut(s) 120, 301, 408
Sau3AI GATC 1 cut(s) 418
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 2 cut(s) 650, 784
SmlI CTYRAG 2 cut(s) 716, 798
SmoI CTYRAG 2 cut(s) 716, 798
SphI GCATGC 1 cut(s) 356
Sse9I AATT 3 cut(s) 291, 470, 530
SseBI AGGCCT 1 cut(s) 740
SsiI CCGC 2 cut(s) 104, 408
StuI AGGCCT 1 cut(s) 740
StyD4I CCNGG 2 cut(s) 648, 782
StyI CCWWGG 2 cut(s) 367, 564
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 2 cut(s) 93, 322
TaqI TCGA 2 cut(s) 376, 764
TasI AATT 3 cut(s) 291, 470, 530
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 746, 787
Tru1I TTAA 3 cut(s) 503, 533, 844
Tru9I TTAA 3 cut(s) 503, 533, 844
TscAI CASTG 1 cut(s) 238
TseI GCWGC 2 cut(s) 119, 300
TspDTI ATGAA 4 cut(s) 114, 153, 349, 440
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 238
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.