FvH4_6g27970

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010955
Physical Location & Seq
Forward (+)
21510185 .. 21516717
6533 bp
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UTR
Exon/CDS
Intron
FvH4_6g27970.t5

Sequence Viewer

Length: 1287 bp
ATGACTCTCTGCAACTCATTTCTTCTCAAAACGCCAATCTTCCCTCCTCACTCCCACTACTTCCCTCCCCAATTTCCCACCAAACCCATCTTCTCCCTCTCCTGCTCCGCCAACCCCTCCTCTTCTTCCGACGACAACGCCACCAATGGCGGTGGTGAGCCCAAGACGAAGAAGAAGCTCTCGGACCAGTCCTCGTGGGAGGCTGAAGACTCTTCGGGTGAGGACTACTTATACCGGCTCGGCAAAGAGGCCGATAACATGAACATCGCCGTTGGAGCCCGAGCCGGCGTCGTCGATGATCTTTTCGTCGGCCAATTCCTCGGCCGTGACTCCGATATCGTCTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTCCAAGGCGATTATTACATTGCGCCTCTCTTCATGGATAAAGTCGTATGCCATATGACGAAGAACTATCTTGCTCCTATTCTTAATACGAAAGTTCCTTTGATCCTAGGCATTTGGGGAGGAAAAGGCCAAGGAAAGTCATTTCAGACTGAACTTATATTTCAAGCTATGGGAGTTGAACCTGTAATTATGTCTGCTGGGGAATTAGAATCAGAGAGAGCTGGGGAACCAGGAAAATTGATACGGGAGCGCTATAGGACAGCTTCCCAAGTTGTCCAGAACCAAGGAAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGCCTTGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATTGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGAATGGCGAGACTCTGATATCACAAATAGAGTTCCTGTCATTGTTACAGGAAATGATTTTTCAAAGATTTATGCTCCCTTAATCCGTGATGGAAGGATGGAAAAGTTTTACTGGCAACCTAACCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTGGACACTTTTCCTAACCAAGCACTGGATTTCTATGGAGCTCTACGGTCTCGAACATATGACAGATCAATTTTAAAGTGGGTTGATGATATTGGTGGTGTTGAAAGTCTTGGAAACCAACTTTTGAAGCGGAGAAAGGATGACAATCTTCCTGTATATACTGCCCCAAAGCAAACTGTGGAGGCTTTGCTTGAATCCGGGTATTCTCTACTGAAAGAACAGCAATTGGTAATGGAGAGCAAACTCTCAAAGGAATACATGAAGAACATGGATGATTAG

Protein Analysis

429

Amino Acids

48.19

Weight (kDa)

5.42

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 20 - 164 1.8e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 167 - 265 8e-34 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1033
AciI CCGC 3 cut(s) 108, 150, 1138
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 2 cut(s) 310, 322
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 380
AfeI AGCGCT 1 cut(s) 632
AfiI CCNNNNNNNGG 1 cut(s) 1033
AgsI TTSAA 6 cut(s) 545, 560, 873, 1112, 1135, 1202
AjnI CCWGG 3 cut(s) 365, 610, 986
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 6 cut(s) 178, 548, 602, 644, 678, 1049
AluI AGCT 6 cut(s) 178, 548, 602, 644, 678, 1049
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 2 cut(s) 813, 1062
AlwI GGATC 1 cut(s) 478
Ama87I CYCGRG 1 cut(s) 279
Aor51HI AGCGCT 1 cut(s) 632
AoxI GGCC 5 cut(s) 249, 310, 322, 508, 706
AspA2I CCTAGG 1 cut(s) 487
AspLEI GCGC 2 cut(s) 406, 633
AspS9I GGNCC 2 cut(s) 184, 369
AsuC2I CCSGG 1 cut(s) 1207
AsuHPI GGTGA 3 cut(s) 167, 230, 355
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 2 cut(s) 184, 369
AvrII CCTAGG 1 cut(s) 487
BanII GRGCYC 3 cut(s) 162, 280, 1051
BauI CACGAG 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 1051
BccI CCATC 4 cut(s) 95, 893, 901, 971
BceAI ACGGC 2 cut(s) 254, 309
BciT130I CCWGG 3 cut(s) 367, 612, 988
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 1 cut(s) 1207
BcoDI GTCTC 2 cut(s) 813, 1062
BfaI CTAG 1 cut(s) 488
BfmI CTRYAG 1 cut(s) 634
BfoI RGCGCY 1 cut(s) 634
BlnI CCTAGG 1 cut(s) 487
Bme1390I CCNGG 4 cut(s) 367, 612, 988, 1207
Bme18I GGWCC 2 cut(s) 184, 369
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 184, 369
BmiI GGNNCC 2 cut(s) 277, 609
BmrFI CCNGG 4 cut(s) 367, 612, 988, 1207
BmsI GCATC 1 cut(s) 691
BpiI GAAGAC 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 1207
BsaBI GATNNNNATC 1 cut(s) 1152
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 1062
BsaJI CCNNGG 7 cut(s) 319, 385, 487, 511, 664, 709, 987
Bsc4I CCNNNNNNNGG 1 cut(s) 1033
Bse118I RCCGGY 2 cut(s) 234, 284
Bse1I ACTGG 4 cut(s) 187, 376, 926, 1038
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 1152
BseBI CCWGG 3 cut(s) 367, 612, 988
BseDI CCNNGG 7 cut(s) 319, 385, 487, 511, 664, 709, 987
BseGI GGATG 4 cut(s) 912, 997, 1153, 1285
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 1 cut(s) 1033
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 743
BseNI ACTGG 4 cut(s) 187, 376, 926, 1038
BseRI GAGGAG 2 cut(s) 36, 109
BseX3I CGGCCG 1 cut(s) 322
BseYI CCCAGC 2 cut(s) 578, 602
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 5 cut(s) 251, 312, 324, 510, 708
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 3 cut(s) 235, 285, 1206
BslI CCNNNNNNNGG 1 cut(s) 1033
BsmAI GTCTC 2 cut(s) 813, 1062
BsnI GGCC 5 cut(s) 251, 312, 324, 510, 708
Bso31I GGTCTC 1 cut(s) 1062
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 162, 280, 1051
Bsp143I GATC 4 cut(s) 298, 483, 687, 1073
BspACI CCGC 3 cut(s) 108, 150, 1138
BspANI GGCC 5 cut(s) 251, 312, 324, 510, 708
BspCNI CTCAG 1 cut(s) 742
BspLI GGNNCC 2 cut(s) 277, 609
BspPI GGATC 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 1062
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 2 cut(s) 234, 284
BsrI ACTGG 4 cut(s) 187, 376, 926, 1038
BssAI RCCGGY 2 cut(s) 234, 284
BssECI CCNNGG 7 cut(s) 319, 385, 487, 511, 664, 709, 987
BssMI GATC 4 cut(s) 298, 483, 687, 1073
BssNI GRCGYC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 5 cut(s) 385, 487, 511, 664, 709
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 3 cut(s) 367, 612, 988
Bst4CI ACNGT 4 cut(s) 739, 935, 1056, 1186
Bst6I CTCTTC 3 cut(s) 127, 217, 416
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 286, 706
BstDEI CTNAG 1 cut(s) 729
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 4 cut(s) 912, 997, 1153, 1285
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 2 cut(s) 406, 633
BstKTI GATC 4 cut(s) 301, 486, 690, 1076
BstMAI GTCTC 2 cut(s) 813, 1062
BstMBI GATC 4 cut(s) 298, 483, 687, 1073
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 3 cut(s) 367, 612, 988
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 4 cut(s) 365, 610, 986, 1205
BstSFI CTRYAG 1 cut(s) 634
BstV2I GAAGAC 1 cut(s) 213
BstZI CGGCCG 1 cut(s) 322
BsuRI GGCC 5 cut(s) 251, 312, 324, 510, 708
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 912, 997, 1153, 1285
BtsIMutI CAGTG 1 cut(s) 1031
Cac8I GCNNGC 2 cut(s) 286, 706
CfoI GCGC 2 cut(s) 406, 633
Cfr10I RCCGGY 2 cut(s) 234, 284
Cfr13I GGNCC 2 cut(s) 184, 369
CseI GACGC 1 cut(s) 277
CsiI ACCWGGT 1 cut(s) 365
Csp6I GTAC 1 cut(s) 379
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 4 cut(s) 259, 415, 1267, 1276
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 729
DpnI GATC 4 cut(s) 300, 485, 689, 1075
DpnII GATC 4 cut(s) 298, 483, 687, 1073
DraI TTTAAA 1 cut(s) 1083
EaeI YGGCCR 2 cut(s) 310, 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 3 cut(s) 127, 217, 416
EarI CTCTTC 3 cut(s) 127, 217, 416
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 1049
EclXI CGGCCG 1 cut(s) 322
Eco130I CCWWGG 5 cut(s) 385, 487, 511, 664, 709
Eco24I GRGCYC 3 cut(s) 162, 280, 1051
Eco31I GGTCTC 1 cut(s) 1062
Eco32I GATATC 3 cut(s) 337, 829, 943
Eco47I GGWCC 2 cut(s) 184, 369
Eco47III AGCGCT 1 cut(s) 632
Eco52I CGGCCG 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 279
Eco91I GGTNACC 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 365, 610, 986
EcoRV GATATC 3 cut(s) 337, 829, 943
EcoT14I CCWWGG 5 cut(s) 385, 487, 511, 664, 709
EcoT38I GRGCYC 3 cut(s) 162, 280, 1051
ErhI CCWWGG 5 cut(s) 385, 487, 511, 664, 709
FaeI CATG 4 cut(s) 262, 418, 1270, 1279
FatI CATG 4 cut(s) 258, 414, 1266, 1275
FauNDI CATATG 2 cut(s) 435, 1066
FbaI TGATCA 1 cut(s) 687
FokI GGATG 3 cut(s) 919, 1004, 1160
FriOI GRGCYC 3 cut(s) 162, 280, 1051
FspBI CTAG 1 cut(s) 488
GlaI GCGC 2 cut(s) 405, 632
GsaI CCCAGC 2 cut(s) 582, 606
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 5 cut(s) 251, 312, 324, 510, 708
HapII CCGG 3 cut(s) 235, 285, 1206
HgaI GACGC 1 cut(s) 277
HhaI GCGC 2 cut(s) 406, 633
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 4 cut(s) 262, 418, 1270, 1279
Hin6I GCGC 2 cut(s) 404, 631
HinP1I GCGC 2 cut(s) 404, 631
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HinfI GANTC 7 cut(s) 4, 209, 329, 590, 772, 821, 1202
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 3 cut(s) 235, 285, 1206
HphI GGTGA 3 cut(s) 167, 230, 355
Hpy166II GTNNAC 2 cut(s) 958, 1012
Hpy188I TCNGA 7 cut(s) 130, 184, 334, 528, 595, 732, 826
Hpy188III TCNNGA 2 cut(s) 658, 1059
Hpy8I GTNNAC 2 cut(s) 958, 1012
Hpy99I CGWCG 4 cut(s) 134, 293, 296, 311
HpyAV CCTTC 3 cut(s) 352, 382, 897
HpyCH4III ACNGT 4 cut(s) 739, 935, 1056, 1186
HpyCH4V TGCA 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 729
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 4 cut(s) 262, 418, 1270, 1279
HspAI GCGC 2 cut(s) 404, 631
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 687
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 4 cut(s) 298, 483, 687, 1073
LmnI GCTCC 6 cut(s) 110, 275, 460, 628, 889, 1046
LweI GCATC 1 cut(s) 691
MabI ACCWGGT 1 cut(s) 365
MaeI CTAG 1 cut(s) 488
MaeIII GTNAC 3 cut(s) 326, 361, 853
MalI GATC 4 cut(s) 300, 485, 689, 1075
MboI GATC 4 cut(s) 298, 483, 687, 1073
MfeI CAATTG 1 cut(s) 1232
MhlI GDGCHC 3 cut(s) 162, 280, 1051
MluCI AATT 8 cut(s) 71, 314, 567, 584, 617, 750, 1077, 1232
MlyI GAGTC 3 cut(s) 203, 323, 815
MmeI TCCRAC 3 cut(s) 153, 253, 739
MroNI GCCGGC 1 cut(s) 284
MseI TTAA 5 cut(s) 465, 890, 948, 957, 1082
MslI CAYNNNNRTG 1 cut(s) 731
MspI CCGG 3 cut(s) 235, 285, 1206
MspR9I CCNGG 4 cut(s) 367, 612, 988, 1207
MunI CAATTG 1 cut(s) 1232
MvaI CCWGG 3 cut(s) 367, 612, 988
MwoI GCNNNNNNNGC 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 286
NciI CCSGG 1 cut(s) 1207
NdeI CATATG 2 cut(s) 435, 1066
NdeII GATC 4 cut(s) 298, 483, 687, 1073
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 4 cut(s) 262, 418, 1270, 1279
NlaIV GGNNCC 2 cut(s) 277, 609
NmeAIII GCCGAG 2 cut(s) 219, 300
NmuCI GTSAC 2 cut(s) 326, 361
PdiI GCCGGC 1 cut(s) 286
PfeI GAWTC 3 cut(s) 590, 772, 1202
PflMI CCANNNNNTGG 1 cut(s) 1033
PleI GAGTC 3 cut(s) 203, 323, 815
PpsI GAGTC 3 cut(s) 203, 323, 815
PpuMI RGGWCCY 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 1051
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 365, 610, 986
PspEI GGTNACC 1 cut(s) 361
PspFI CCCAGC 2 cut(s) 578, 602
PspGI CCWGG 3 cut(s) 365, 610, 986
PspN4I GGNNCC 2 cut(s) 277, 609
PspPI GGNCC 2 cut(s) 184, 369
PspPPI RGGWCCY 1 cut(s) 369
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 1051
SaqAI TTAA 5 cut(s) 465, 890, 948, 957, 1082
Sau3AI GATC 4 cut(s) 298, 483, 687, 1073
Sau96I GGNCC 2 cut(s) 184, 369
SchI GAGTC 3 cut(s) 203, 323, 815
ScrFI CCNGG 4 cut(s) 367, 612, 988, 1207
SduI GDGCHC 3 cut(s) 162, 280, 1051
SexAI ACCWGGT 1 cut(s) 365
SfaNI GCATC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 634
SinI GGWCC 2 cut(s) 184, 369
SmiMI CAYNNNNRTG 1 cut(s) 731
Sse9I AATT 8 cut(s) 71, 314, 567, 584, 617, 750, 1077, 1232
SsiI CCGC 3 cut(s) 108, 150, 1138
SspMI CTAG 1 cut(s) 488
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 4 cut(s) 365, 610, 986, 1205
StyI CCWWGG 5 cut(s) 385, 487, 511, 664, 709
TaaI ACNGT 4 cut(s) 739, 935, 1056, 1186
TaqI TCGA 2 cut(s) 294, 1060
TasI AATT 8 cut(s) 71, 314, 567, 584, 617, 750, 1077, 1232
TfiI GAWTC 3 cut(s) 590, 772, 1202
Tru1I TTAA 5 cut(s) 465, 890, 948, 957, 1082
Tru9I TTAA 5 cut(s) 465, 890, 948, 957, 1082
TscAI CASTG 1 cut(s) 1038
TseFI GTSAC 2 cut(s) 326, 361
Tsp45I GTSAC 2 cut(s) 326, 361
TspDTI ATGAA 5 cut(s) 275, 403, 785, 1008, 1283
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 2 cut(s) 184, 369
XmaJI CCTAGG 1 cut(s) 487
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.