Rh2DG375500

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010955
Physical Location & Seq
Forward (+)
53615049 .. 53621904
6856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG375500.1

Sequence Viewer

Length: 1311 bp
ATGACTCTCTGCAACTCATTTCTCCTCAAAACCCCAATCTTCCCTCACTCCCATTTCTTCACTCACCAATTTCCCACCAAACCCATCTTCTCCCTTTCCTGCTCCGCAACCCCCTCTTCCTCCTCCTCCGTGCCTGACCCCAAAACCAACGCCAGCGACGGCAACGCCGCCAATGGCGGCGGTGAGCCGAAGAGCAAGAGGCTTTCGGACCAGTCCTCGTGGGAGGCCAAGGACTCTGTGGGCGAGGACTACTTATACAGGCTCGGCAAAGAGGCCGACAACATGAACATCGCCGTCGGAGCCCGGGCCGGCGTCGTCGATGACCTTTTCGTCGGGAAATTCCTCGGCCGTGACTCCGATATCGTTTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTGCAAGGCGATTATTACATTGCGCCTCTCTTCATGGATAAAGTCGTATGCCATATGGCGAAGAACTACCTTGCTCATATTCTCAATACGAAAGTTCCTTTGATTCTAGGTATTTGGGGAGGAAAAGGCCAAGGAAAGTCTTTTCAGACTGAACTTGTATTTCAAGCTATGGGAGTTGAACCTGTAGTTATGTCTGCTGGGGAATTAGAATCAGAAAGAGCTGGGGAACCAGGGAAATTGATACGGGAACGCTATAGAACAGCTTCTCAAGTTGTCCAGAACCAAGGCAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGGCTCGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATTGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGACTGGCGAGAATCAGATATCACAAACAGAGTTCCTATCATTGTTACAGGAAATGATTTTTCAAAAATTTATGCTCCCTTAATCCGTGATGGAAGGATGGAAAAGTTTTATTGGCAACCTAATCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTTGACACTTTTCCTAACCAAGCACTGGATTTCTATGGAGCTCTACGGTCTCGAACATATGATCGGTCAATTTCTAAGTGGATTGATGATATTGGCGGTGTTGAAAGTCTTGGAAACAAACTTGTGAAGCGAAGAAAGGATGACAATCTTCCTGTATATACTCCTCCAAAGCAAACGATGGAGGCTTTGCTTGAATCCGGGTATTCTCTGCTGAAAGAACAGCAGTTGATAATGGAGACCAAACTTTCAAAGGAATACATGAAGAACATGGATGATTAG

Protein Analysis

436

Amino Acids

48.97

Weight (kDa)

6.28

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 173 - 317 8.9e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 320 - 418 1.9e-34 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
Acc36I ACCTGC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 1057
AciI CCGC 5 cut(s) 105, 168, 177, 180, 1128
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 338, 900
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 1057
AgsI TTSAA 6 cut(s) 569, 584, 897, 1136, 1226, 1281
AjnI CCWGG 3 cut(s) 389, 634, 1010
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 5 cut(s) 572, 626, 668, 702, 1073
AluI AGCT 5 cut(s) 572, 626, 668, 702, 1073
Alw21I GWGCWC 1 cut(s) 1075
Alw26I GTCTC 2 cut(s) 1086, 1262
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 5 cut(s) 225, 273, 306, 346, 532
ApoI RAATTY 2 cut(s) 338, 900
Asp700I GAANNNNTTC 1 cut(s) 667
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 3 cut(s) 208, 306, 393
AsuC2I CCSGG 3 cut(s) 304, 305, 1231
AsuHPI GGTGA 3 cut(s) 56, 194, 379
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 2 cut(s) 208, 393
BanII GRGCYC 3 cut(s) 304, 735, 1075
BauI CACGAG 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 1075
BccI CCATC 5 cut(s) 92, 917, 925, 995, 1204
BceAI ACGGC 3 cut(s) 175, 278, 333
BcgI CGANNNNNNTGC 2 cut(s) 938, 972
BciT130I CCWGG 3 cut(s) 391, 636, 1012
BclI TGATCA 1 cut(s) 711
BcnI CCSGG 3 cut(s) 304, 305, 1231
BcoDI GTCTC 2 cut(s) 1086, 1262
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 2 cut(s) 588, 658
BfuAI ACCTGC 1 cut(s) 414
BisI GCNGC 2 cut(s) 168, 178
BlsI GCNGC 2 cut(s) 169, 179
Bme1390I CCNGG 6 cut(s) 304, 305, 391, 636, 1012, 1231
Bme18I GGWCC 2 cut(s) 208, 393
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 3 cut(s) 208, 306, 393
BmiI GGNNCC 2 cut(s) 301, 633
BmrFI CCNGG 6 cut(s) 304, 305, 391, 636, 1012, 1231
BmsI GCATC 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 3 cut(s) 304, 305, 1231
BsaBI GATNNNNATC 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 312
BsaI GGTCTC 2 cut(s) 1086, 1262
BsaJI CCNNGG 7 cut(s) 228, 303, 343, 535, 635, 688, 1011
Bsc4I CCNNNNNNNGG 1 cut(s) 1057
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 4 cut(s) 211, 400, 842, 1062
Bse3DI GCAATG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 3 cut(s) 391, 636, 1012
BseDI CCNNGG 7 cut(s) 228, 303, 343, 535, 635, 688, 1011
BseGI GGATG 4 cut(s) 936, 1021, 1177, 1309
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 1 cut(s) 1057
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 767
BseNI ACTGG 4 cut(s) 211, 400, 842, 1062
BseRI GAGGAG 4 cut(s) 14, 112, 115, 1185
BseX3I CGGCCG 1 cut(s) 346
BseYI CCCAGC 3 cut(s) 602, 626, 728
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 5 cut(s) 227, 275, 308, 348, 534
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 1075
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 3 cut(s) 304, 309, 1230
BslI CCNNNNNNNGG 1 cut(s) 1057
BsmAI GTCTC 2 cut(s) 1086, 1262
BsnI GGCC 5 cut(s) 227, 275, 308, 348, 534
Bso31I GGTCTC 2 cut(s) 1086, 1262
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 3 cut(s) 304, 735, 1075
Bsp143I GATC 2 cut(s) 711, 1093
BspACI CCGC 5 cut(s) 105, 168, 177, 180, 1128
BspANI GGCC 5 cut(s) 227, 275, 308, 348, 534
BspCNI CTCAG 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 301, 633
BspMI ACCTGC 1 cut(s) 414
BspQI GCTCTTC 1 cut(s) 185
BspTNI GGTCTC 2 cut(s) 1086, 1262
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 4 cut(s) 211, 400, 842, 1062
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 7 cut(s) 228, 303, 343, 535, 635, 688, 1011
BssMI GATC 2 cut(s) 711, 1093
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 217
BssT1I CCWWGG 3 cut(s) 228, 535, 688
Bst2BI CACGAG 1 cut(s) 217
Bst2UI CCWGG 3 cut(s) 391, 636, 1012
Bst4CI ACNGT 2 cut(s) 763, 1080
Bst6I CTCTTC 3 cut(s) 121, 185, 440
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 154, 310
BstDEI CTNAG 2 cut(s) 753, 1107
BstEII GGTNACC 1 cut(s) 385
BstF5I GGATG 4 cut(s) 936, 1021, 1177, 1309
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 2 cut(s) 714, 1096
BstMAI GTCTC 2 cut(s) 1086, 1262
BstMBI GATC 2 cut(s) 711, 1093
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 2 cut(s) 299, 699
BstNI CCWGG 3 cut(s) 391, 636, 1012
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 6 cut(s) 302, 303, 389, 634, 1010, 1229
BstSFI CTRYAG 2 cut(s) 588, 658
BstZI CGGCCG 1 cut(s) 346
BsuRI GGCC 5 cut(s) 227, 275, 308, 348, 534
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 936, 1021, 1177, 1309
BtsIMutI CAGTG 1 cut(s) 1055
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 154, 310
CaiI CAGNNNCTG 1 cut(s) 406
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 208, 306, 393
Cfr9I CCCGGG 1 cut(s) 303
CseI GACGC 1 cut(s) 301
CsiI ACCWGGT 1 cut(s) 389
Csp6I GTAC 1 cut(s) 403
CviAII CATG 4 cut(s) 283, 439, 1291, 1300
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 753, 1107
DpnI GATC 2 cut(s) 713, 1095
DpnII GATC 2 cut(s) 711, 1093
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
EaeI YGGCCR 1 cut(s) 346
EagI CGGCCG 1 cut(s) 346
Eam1104I CTCTTC 3 cut(s) 121, 185, 440
EarI CTCTTC 3 cut(s) 121, 185, 440
Ecl136II GAGCTC 1 cut(s) 1073
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 3 cut(s) 228, 535, 688
Eco24I GRGCYC 3 cut(s) 304, 735, 1075
Eco31I GGTCTC 2 cut(s) 1086, 1262
Eco32I GATATC 3 cut(s) 361, 853, 967
Eco47I GGWCC 2 cut(s) 208, 393
Eco52I CGGCCG 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 1073
Eco88I CYCGRG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 1073
EcoO109I RGGNCCY 1 cut(s) 393
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 389, 634, 1010
EcoRV GATATC 3 cut(s) 361, 853, 967
EcoT14I CCWWGG 3 cut(s) 228, 535, 688
EcoT38I GRGCYC 3 cut(s) 304, 735, 1075
ErhI CCWWGG 3 cut(s) 228, 535, 688
FaeI CATG 4 cut(s) 286, 442, 1294, 1303
FatI CATG 4 cut(s) 282, 438, 1290, 1299
FauNDI CATATG 2 cut(s) 459, 1090
FbaI TGATCA 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 168, 178
FokI GGATG 3 cut(s) 943, 1028, 1184
FriOI GRGCYC 3 cut(s) 304, 735, 1075
Fsp4HI GCNGC 2 cut(s) 168, 178
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 429
GluI GCNGC 2 cut(s) 168, 178
GsaI CCCAGC 3 cut(s) 606, 630, 732
HaeIII GGCC 5 cut(s) 227, 275, 308, 348, 534
HapII CCGG 3 cut(s) 304, 309, 1230
HgaI GACGC 1 cut(s) 301
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 4 cut(s) 286, 442, 1294, 1303
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 2 cut(s) 982, 1036
HindII GTYRAC 2 cut(s) 982, 1036
HinfI GANTC 8 cut(s) 4, 233, 353, 508, 614, 796, 845, 1226
HpaI GTTAAC 1 cut(s) 982
HpaII CCGG 3 cut(s) 304, 309, 1230
HphI GGTGA 3 cut(s) 56, 194, 379
Hpy166II GTNNAC 2 cut(s) 982, 1036
Hpy188I TCNGA 7 cut(s) 208, 299, 358, 552, 619, 756, 850
Hpy188III TCNNGA 4 cut(s) 334, 682, 959, 1083
Hpy8I GTNNAC 2 cut(s) 982, 1036
Hpy99I CGWCG 5 cut(s) 161, 299, 317, 320, 335
HpyAV CCTTC 3 cut(s) 376, 406, 921
HpyCH4III ACNGT 2 cut(s) 763, 1080
HpyCH4V TGCA 2 cut(s) 12, 409
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 699
HpyF3I CTNAG 2 cut(s) 753, 1107
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 4 cut(s) 286, 442, 1294, 1303
HspAI GCGC 1 cut(s) 428
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 711
KspAI GTTAAC 1 cut(s) 982
Kzo9I GATC 2 cut(s) 711, 1093
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 4 cut(s) 107, 299, 913, 1070
LweI GCATC 1 cut(s) 715
MabI ACCWGGT 1 cut(s) 389
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 3 cut(s) 350, 385, 877
MalI GATC 2 cut(s) 713, 1095
MboI GATC 2 cut(s) 711, 1093
MhlI GDGCHC 3 cut(s) 304, 735, 1075
MluCI AATT 7 cut(s) 68, 338, 608, 641, 774, 900, 1101
MlyI GAGTC 2 cut(s) 227, 347
MmeI TCCRAC 2 cut(s) 277, 763
MroNI GCCGGC 1 cut(s) 308
MroXI GAANNNNTTC 1 cut(s) 667
MseI TTAA 3 cut(s) 914, 972, 981
MslI CAYNNNNRTG 1 cut(s) 755
MspI CCGG 3 cut(s) 304, 309, 1230
MspR9I CCNGG 6 cut(s) 304, 305, 391, 636, 1012, 1231
MvaI CCWGG 3 cut(s) 391, 636, 1012
MwoI GCNNNNNNNGC 2 cut(s) 299, 699
NaeI GCCGGC 1 cut(s) 310
NciI CCSGG 3 cut(s) 304, 305, 1231
NdeI CATATG 2 cut(s) 459, 1090
NdeII GATC 2 cut(s) 711, 1093
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 4 cut(s) 286, 442, 1294, 1303
NlaIV GGNNCC 2 cut(s) 301, 633
NmeAIII GCCGAG 2 cut(s) 243, 324
NmuCI GTSAC 2 cut(s) 350, 385
PciSI GCTCTTC 1 cut(s) 185
PdiI GCCGGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 667
PfeI GAWTC 5 cut(s) 508, 614, 796, 845, 1226
PflMI CCANNNNNTGG 1 cut(s) 1057
PkrI GCNGC 2 cut(s) 169, 179
PleI GAGTC 2 cut(s) 227, 347
PpsI GAGTC 2 cut(s) 227, 347
PpuMI RGGWCCY 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 1075
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 3 cut(s) 389, 634, 1010
PspEI GGTNACC 1 cut(s) 385
PspFI CCCAGC 3 cut(s) 602, 626, 728
PspGI CCWGG 3 cut(s) 389, 634, 1010
PspN4I GGNNCC 2 cut(s) 301, 633
PspPI GGNCC 3 cut(s) 208, 306, 393
PspPPI RGGWCCY 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 406
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 755
SacI GAGCTC 1 cut(s) 1075
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 914, 972, 981
SatI GCNGC 2 cut(s) 168, 178
Sau3AI GATC 2 cut(s) 711, 1093
Sau96I GGNCC 3 cut(s) 208, 306, 393
SchI GAGTC 2 cut(s) 227, 347
ScrFI CCNGG 6 cut(s) 304, 305, 391, 636, 1012, 1231
SduI GDGCHC 3 cut(s) 304, 735, 1075
SexAI ACCWGGT 1 cut(s) 389
SfaNI GCATC 1 cut(s) 715
SfcI CTRYAG 2 cut(s) 588, 658
SinI GGWCC 2 cut(s) 208, 393
SmaI CCCGGG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
SrfI GCCCGGGC 1 cut(s) 305
Sse9I AATT 7 cut(s) 68, 338, 608, 641, 774, 900, 1101
SsiI CCGC 5 cut(s) 105, 168, 177, 180, 1128
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 1 cut(s) 1075
StyD4I CCNGG 6 cut(s) 302, 303, 389, 634, 1010, 1229
StyI CCWWGG 3 cut(s) 228, 535, 688
TaaI ACNGT 2 cut(s) 763, 1080
TaqI TCGA 2 cut(s) 318, 1084
TaqII GACCGA 1 cut(s) 1086
TasI AATT 7 cut(s) 68, 338, 608, 641, 774, 900, 1101
TauI GCSGC 2 cut(s) 170, 180
TfiI GAWTC 5 cut(s) 508, 614, 796, 845, 1226
Tru1I TTAA 3 cut(s) 914, 972, 981
Tru9I TTAA 3 cut(s) 914, 972, 981
TscAI CASTG 1 cut(s) 1062
TseFI GTSAC 2 cut(s) 350, 385
Tsp45I GTSAC 2 cut(s) 350, 385
TspDTI ATGAA 5 cut(s) 299, 427, 809, 1032, 1307
TspGWI ACGGA 2 cut(s) 118, 908
TspMI CCCGGG 1 cut(s) 303
TspRI CASTG 1 cut(s) 1062
Van91I CCANNNNNTGG 1 cut(s) 1057
VpaK11BI GGWCC 2 cut(s) 208, 393
XapI RAATTY 2 cut(s) 338, 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmaI CCCGGG 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 667
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.