Rroxscaffold_2G00112760

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010955
Physical Location & Seq
Reverse (-)
38079579 .. 38086172
6594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00112760.1

Sequence Viewer

Length: 1317 bp
ATGACTCTCTGTAACTCATTTCTCCTCAAAACCCCAATCTTCCCTCACTCCCATTTCTTAACTCACCAATTTCCCACCAACAAACCCATCTTCTCCCTCTCCTGCTCCACAACCCCCTCTCCTTCTTCCTCCTCCGTCCCTGACCCCAAAACCAACGCCAGCGACGGCAGCGCCACCAATGGCGGTGGTGAGCCGAAGAGCAAGAGGCTTTCGGACCAGTCCTCGTGGGAGGCCAAGGACTCTGTGGGCGAGGACTACTTATACAGGCTCGGCAAAGAGGCTGATAACATGAACATCGCCGTCGGAGCCCGGGCCGGCGTCGTCGATGACCTTTTCGTCGGGAAATTCCTTGGCCGTGACTCCGATATCGTCTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTGCAAGGCGATTATTACATTGCGCCTCTCTTCATGTTAAGTGCAGTATGCCATATGGCGAAGAACTACCTTGCTCATATTCTCAATACGAAAGTTCCTTTGATTCTAGGTATTTGGGGAGGCAAAGGCCAAGGAAAGTCATTTCAGACTGAACTTGTATTTCAAGCTATGGGAGTTGAACCTGTAGTTATGTCTGCTGGGGAATTAGAATCAGAAAGAGCTGGGGAACCAGGGAAATTGATACGGGAACGCTATAGAACAGCTTCTCAAGTTGTCCAGAACCAAGGCAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGGCTTGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATTGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGACTGGCGAGAATCAGATATCACAAACAGAGTTCCTATCATTGTTACAGGAAATGATTTTTCAAAAATTTATGCTCCCTTAATCCGTGATGGAAGGATGGAAAAGTTTTATTGGCAACCTAACCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTTGACACTTTTCCTAACCAAGCACTGGATTTCTATGGAGCTCTACGGTCTCGAACATATGATCGGTCAATTTCTAAGTGGATTGATGATATTGGTGGTGTTGAAAGTCTTGGAAACAAACTTGTGAAGCGAAGAAAGGATGACAATCTTCCTGTATATACTCCTCCAAAGCAAACGATGGAGGCTTTGCTTGAATCCGGGTATTCTCTGCTGAAAGAACAGCAGTTGATAATGGAGACCAAACTTTCAAAGGAATACATGAAGAACATGGATGATTAG

Protein Analysis

438

Amino Acids

49.1

Weight (kDa)

6.28

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 175 - 319 9e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 322 - 420 1.9e-34 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 420
AccB7I CCANNNNNTGG 1 cut(s) 1063
AciI CCGC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 2 cut(s) 344, 906
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 1063
AgsI TTSAA 6 cut(s) 575, 590, 903, 1142, 1232, 1287
AjnI CCWGG 3 cut(s) 395, 640, 1016
AluBI AGCT 5 cut(s) 578, 632, 674, 708, 1079
AluI AGCT 5 cut(s) 578, 632, 674, 708, 1079
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 2 cut(s) 1092, 1268
AlwNI CAGNNNCTG 1 cut(s) 412
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 4 cut(s) 231, 312, 352, 538
ApeKI GCWGC 1 cut(s) 168
ApoI RAATTY 2 cut(s) 344, 906
Asp700I GAANNNNTTC 1 cut(s) 673
AspLEI GCGC 2 cut(s) 173, 436
AspS9I GGNCC 3 cut(s) 214, 312, 399
AsuC2I CCSGG 3 cut(s) 310, 311, 1237
AsuHPI GGTGA 3 cut(s) 56, 200, 385
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 2 cut(s) 214, 399
BanII GRGCYC 2 cut(s) 310, 1081
BauI CACGAG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 1 cut(s) 180
BccI CCATC 5 cut(s) 95, 923, 931, 1001, 1210
BceAI ACGGC 3 cut(s) 181, 284, 339
BciT130I CCWGG 3 cut(s) 397, 642, 1018
BclI TGATCA 1 cut(s) 717
BcnI CCSGG 3 cut(s) 310, 311, 1237
BcoDI GTCTC 2 cut(s) 1092, 1268
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 2 cut(s) 594, 664
BfoI RGCGCY 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 420
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
Bme1390I CCNGG 6 cut(s) 310, 311, 397, 642, 1018, 1237
Bme18I GGWCC 2 cut(s) 214, 399
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 3 cut(s) 214, 312, 399
BmiI GGNNCC 2 cut(s) 307, 639
BmrFI CCNGG 6 cut(s) 310, 311, 397, 642, 1018, 1237
BmsI GCATC 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 663
BpuMI CCSGG 3 cut(s) 310, 311, 1237
BsaBI GATNNNNATC 1 cut(s) 1182
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 2 cut(s) 1092, 1268
BsaJI CCNNGG 7 cut(s) 234, 309, 349, 541, 641, 694, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 1063
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 4 cut(s) 217, 406, 848, 1068
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 3 cut(s) 397, 642, 1018
BseDI CCNNGG 7 cut(s) 234, 309, 349, 541, 641, 694, 1017
BseGI GGATG 4 cut(s) 942, 1027, 1183, 1315
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 1 cut(s) 1063
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 4 cut(s) 217, 406, 848, 1068
BseRI GAGGAG 3 cut(s) 14, 121, 1191
BseXI GCAGC 1 cut(s) 180
BseYI CCCAGC 3 cut(s) 608, 632, 734
BsgI GTGCAG 1 cut(s) 474
BshFI GGCC 4 cut(s) 233, 314, 354, 540
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 3 cut(s) 310, 315, 1236
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 1063
BsmAI GTCTC 2 cut(s) 1092, 1268
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 4 cut(s) 233, 314, 354, 540
Bso31I GGTCTC 2 cut(s) 1092, 1268
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 310, 1081
Bsp143I GATC 2 cut(s) 717, 1099
BspACI CCGC 1 cut(s) 183
BspANI GGCC 4 cut(s) 233, 314, 354, 540
BspCNI CTCAG 1 cut(s) 772
BspLI GGNNCC 2 cut(s) 307, 639
BspMI ACCTGC 1 cut(s) 420
BspQI GCTCTTC 1 cut(s) 191
BspTNI GGTCTC 2 cut(s) 1092, 1268
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 4 cut(s) 217, 406, 848, 1068
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 7 cut(s) 234, 309, 349, 541, 641, 694, 1017
BssMI GATC 2 cut(s) 717, 1099
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 223
BssT1I CCWWGG 4 cut(s) 234, 349, 541, 694
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 3 cut(s) 397, 642, 1018
Bst4CI ACNGT 3 cut(s) 769, 965, 1086
Bst6I CTCTTC 2 cut(s) 191, 446
BstACI GRCGYC 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 160, 316
BstDEI CTNAG 2 cut(s) 759, 1113
BstEII GGTNACC 1 cut(s) 391
BstF5I GGATG 4 cut(s) 942, 1027, 1183, 1315
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 2 cut(s) 173, 436
BstKTI GATC 2 cut(s) 720, 1102
BstMAI GTCTC 2 cut(s) 1092, 1268
BstMBI GATC 2 cut(s) 717, 1099
BstMWI GCNNNNNNNGC 3 cut(s) 168, 305, 705
BstNI CCWGG 3 cut(s) 397, 642, 1018
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 6 cut(s) 308, 309, 395, 640, 1016, 1235
BstSFI CTRYAG 2 cut(s) 594, 664
BstV1I GCAGC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 233, 314, 354, 540
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 4 cut(s) 942, 1027, 1183, 1315
BtsIMutI CAGTG 1 cut(s) 1061
BveI ACCTGC 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 160, 316
CaiI CAGNNNCTG 1 cut(s) 412
CfoI GCGC 2 cut(s) 173, 436
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 214, 312, 399
Cfr9I CCCGGG 1 cut(s) 309
CseI GACGC 1 cut(s) 307
CsiI ACCWGGT 1 cut(s) 395
Csp6I GTAC 1 cut(s) 409
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 4 cut(s) 289, 445, 1297, 1306
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 2 cut(s) 759, 1113
DpnI GATC 2 cut(s) 719, 1101
DpnII GATC 2 cut(s) 717, 1099
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 191, 446
EarI CTCTTC 2 cut(s) 191, 446
Ecl136II GAGCTC 1 cut(s) 1079
Eco130I CCWWGG 4 cut(s) 234, 349, 541, 694
Eco24I GRGCYC 2 cut(s) 310, 1081
Eco31I GGTCTC 2 cut(s) 1092, 1268
Eco32I GATATC 3 cut(s) 367, 859, 973
Eco47I GGWCC 2 cut(s) 214, 399
Eco53kI GAGCTC 1 cut(s) 1079
Eco88I CYCGRG 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 1079
EcoO109I RGGNCCY 1 cut(s) 399
EcoO65I GGTNACC 1 cut(s) 391
EcoRII CCWGG 3 cut(s) 395, 640, 1016
EcoRV GATATC 3 cut(s) 367, 859, 973
EcoT14I CCWWGG 4 cut(s) 234, 349, 541, 694
EcoT38I GRGCYC 2 cut(s) 310, 1081
ErhI CCWWGG 4 cut(s) 234, 349, 541, 694
FaeI CATG 4 cut(s) 292, 448, 1300, 1309
FaqI GGGAC 1 cut(s) 122
FatI CATG 4 cut(s) 288, 444, 1296, 1305
FauNDI CATATG 2 cut(s) 465, 1096
FbaI TGATCA 1 cut(s) 717
Fnu4HI GCNGC 1 cut(s) 169
FokI GGATG 3 cut(s) 949, 1034, 1190
FriOI GRGCYC 2 cut(s) 310, 1081
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 1 cut(s) 518
GlaI GCGC 2 cut(s) 172, 435
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 3 cut(s) 612, 636, 738
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 4 cut(s) 233, 314, 354, 540
HapII CCGG 3 cut(s) 310, 315, 1236
HgaI GACGC 1 cut(s) 307
HhaI GCGC 2 cut(s) 173, 436
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 4 cut(s) 292, 448, 1300, 1309
Hin6I GCGC 2 cut(s) 171, 434
HinP1I GCGC 2 cut(s) 171, 434
HincII GTYRAC 2 cut(s) 988, 1042
HindII GTYRAC 2 cut(s) 988, 1042
HinfI GANTC 8 cut(s) 4, 239, 359, 514, 620, 802, 851, 1232
HpaI GTTAAC 1 cut(s) 988
HpaII CCGG 3 cut(s) 310, 315, 1236
HphI GGTGA 3 cut(s) 56, 200, 385
Hpy166II GTNNAC 2 cut(s) 988, 1042
Hpy188I TCNGA 7 cut(s) 214, 305, 364, 558, 625, 762, 856
Hpy188III TCNNGA 3 cut(s) 340, 688, 1089
Hpy8I GTNNAC 2 cut(s) 988, 1042
Hpy99I CGWCG 5 cut(s) 167, 305, 323, 326, 341
HpyAV CCTTC 4 cut(s) 132, 382, 412, 927
HpyCH4III ACNGT 3 cut(s) 769, 965, 1086
HpyCH4V TGCA 2 cut(s) 415, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 305, 705
HpyF3I CTNAG 2 cut(s) 759, 1113
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 4 cut(s) 292, 448, 1300, 1309
HspAI GCGC 2 cut(s) 171, 434
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 717
KspAI GTTAAC 1 cut(s) 988
Kzo9I GATC 2 cut(s) 717, 1099
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 4 cut(s) 110, 305, 919, 1076
Lsp1109I GCAGC 1 cut(s) 180
LweI GCATC 1 cut(s) 721
MabI ACCWGGT 1 cut(s) 395
MaeI CTAG 1 cut(s) 518
MaeIII GTNAC 4 cut(s) 11, 356, 391, 883
MalI GATC 2 cut(s) 719, 1101
MboI GATC 2 cut(s) 717, 1099
MboII GAAGA 9 cut(s) 31, 82, 117, 208, 433, 484, 1178, 1182, 1312
MhlI GDGCHC 2 cut(s) 310, 1081
MluCI AATT 7 cut(s) 68, 344, 614, 647, 780, 906, 1107
MlyI GAGTC 2 cut(s) 233, 353
MmeI TCCRAC 2 cut(s) 283, 769
MroNI GCCGGC 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 5 cut(s) 59, 449, 920, 978, 987
MslI CAYNNNNRTG 1 cut(s) 761
MspI CCGG 3 cut(s) 310, 315, 1236
MspR9I CCNGG 6 cut(s) 310, 311, 397, 642, 1018, 1237
MvaI CCWGG 3 cut(s) 397, 642, 1018
MwoI GCNNNNNNNGC 3 cut(s) 168, 305, 705
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 3 cut(s) 310, 311, 1237
NdeI CATATG 2 cut(s) 465, 1096
NdeII GATC 2 cut(s) 717, 1099
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 4 cut(s) 292, 448, 1300, 1309
NlaIV GGNNCC 2 cut(s) 307, 639
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 2 cut(s) 356, 391
PciSI GCTCTTC 1 cut(s) 191
PdiI GCCGGC 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 5 cut(s) 514, 620, 802, 851, 1232
PflMI CCANNNNNTGG 1 cut(s) 1063
PkrI GCNGC 1 cut(s) 170
PleI GAGTC 2 cut(s) 233, 353
PpsI GAGTC 2 cut(s) 233, 353
PpuMI RGGWCCY 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 1081
Psp5II RGGWCCY 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 395, 640, 1016
PspEI GGTNACC 1 cut(s) 391
PspFI CCCAGC 3 cut(s) 608, 632, 734
PspGI CCWGG 3 cut(s) 395, 640, 1016
PspN4I GGNNCC 2 cut(s) 307, 639
PspPI GGNCC 3 cut(s) 214, 312, 399
PspPPI RGGWCCY 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 412
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 761
SacI GAGCTC 1 cut(s) 1081
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 5 cut(s) 59, 449, 920, 978, 987
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 2 cut(s) 717, 1099
Sau96I GGNCC 3 cut(s) 214, 312, 399
SchI GAGTC 2 cut(s) 233, 353
ScrFI CCNGG 6 cut(s) 310, 311, 397, 642, 1018, 1237
SduI GDGCHC 2 cut(s) 310, 1081
SexAI ACCWGGT 1 cut(s) 395
SfaNI GCATC 1 cut(s) 721
SfcI CTRYAG 2 cut(s) 594, 664
SinI GGWCC 2 cut(s) 214, 399
SmaI CCCGGG 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SrfI GCCCGGGC 1 cut(s) 311
Sse9I AATT 7 cut(s) 68, 344, 614, 647, 780, 906, 1107
SsiI CCGC 1 cut(s) 183
SspMI CTAG 1 cut(s) 518
SstI GAGCTC 1 cut(s) 1081
StyD4I CCNGG 6 cut(s) 308, 309, 395, 640, 1016, 1235
StyI CCWWGG 4 cut(s) 234, 349, 541, 694
TaaI ACNGT 3 cut(s) 769, 965, 1086
TaqI TCGA 2 cut(s) 324, 1090
TaqII GACCGA 1 cut(s) 1092
TasI AATT 7 cut(s) 68, 344, 614, 647, 780, 906, 1107
TfiI GAWTC 5 cut(s) 514, 620, 802, 851, 1232
Tru1I TTAA 5 cut(s) 59, 449, 920, 978, 987
Tru9I TTAA 5 cut(s) 59, 449, 920, 978, 987
TscAI CASTG 1 cut(s) 1068
TseFI GTSAC 2 cut(s) 356, 391
TseI GCWGC 1 cut(s) 168
Tsp45I GTSAC 2 cut(s) 356, 391
TspDTI ATGAA 5 cut(s) 305, 433, 815, 1038, 1313
TspGWI ACGGA 2 cut(s) 124, 914
TspMI CCCGGG 1 cut(s) 309
TspRI CASTG 1 cut(s) 1068
Van91I CCANNNNNTGG 1 cut(s) 1063
VpaK11BI GGWCC 2 cut(s) 214, 399
XapI RAATTY 2 cut(s) 344, 906
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmaI CCCGGG 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.