Rh2BG357700

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010955
Physical Location & Seq
Forward (+)
49369342 .. 49376662
7321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG357700.1

Sequence Viewer

Length: 1092 bp
ATGACTCTCTGCAACTCATTTCTCCTCAAAACCCCAATCTTCCCTCACTCCCATTTCTTCACTCACCAATTTCCCACCAAACCCATCTTCTCCCTTTCCTGCTCCGCAACCCCCTCTTCCTCCTCCTCCTCCTCCTCCGTGCCTGACCCCAAAACCAACGCCAACGACGGCGAAGCCGCCAATGGCGGCGGTGAGCCGAAGAGCAAGAGGCTTTCGGACCAGTCCTCGTGGGAGGCCAAGGACTCTGTGGGCGAGGACTACTTATACAGGCTCGGCAAAGAGGCCGACAACATGAACATCGCCGTCGGAGCCCGGGCCGGCGTCGTCGATGACCTTTTCGTCGGGAAATTCCTCGGCCGTGACTCCGATATCGTTTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTCCAAGGCGATTATTACATTGCGCCTCTCTTCATGGATAAAGTCGTATGCCATATGGCGAAGAACTACCTTGCTCATATTCTCAATACGAAAGTTCCTTTGATTCTAGGTATTTGGGGAGGAAAAGGCCAAGGAAAGTCTTTTCAGACTGAACTTGTATTTCAAGCTATGGGAGTTGAACCTGTAGTTATGTCTGCTGGGGAATTAGAATCAGAAAGAGCTGGGGAACCAGGGAAATTGATACGGGAACGCTATAGAACAGCTTCTCAAGTTGTCCAGAACCAAGGCAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGGCTCGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATTGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGACTGGCGAGAATCAGATATCACAAACAGAGTTCCTATCATTGTTACAGGAAATGATTTTTCAAAAATTTATGCTCCCTTAATCCGTGATGGAAGGATGGAAAAGTTTTATTGGCAACCTAATCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTTGACACTTTTCCTAACCAAGGTATGACTCTGCTCTCTTTTCCTCAATGA

Protein Analysis

363

Amino Acids

40.41

Weight (kDa)

6.02

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 176 - 320 6e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 323 - 356 2.3e-08 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AciI CCGC 4 cut(s) 105, 177, 186, 189
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 2 cut(s) 347, 909
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 1061
AgsI TTSAA 3 cut(s) 578, 593, 906
AjnI CCWGG 3 cut(s) 398, 643, 1019
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 581, 635, 677, 711
AluI AGCT 4 cut(s) 581, 635, 677, 711
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 5 cut(s) 234, 282, 315, 355, 541
ApoI RAATTY 2 cut(s) 347, 909
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 217, 315, 402
AsuC2I CCSGG 2 cut(s) 313, 314
AsuHPI GGTGA 3 cut(s) 56, 203, 388
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 217, 402
BanII GRGCYC 2 cut(s) 313, 744
BauI CACGAG 1 cut(s) 226
BccI CCATC 4 cut(s) 92, 926, 934, 1004
BceAI ACGGC 3 cut(s) 184, 287, 342
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 3 cut(s) 400, 645, 1021
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 2 cut(s) 313, 314
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 597, 667
BisI GCNGC 2 cut(s) 177, 187
BlsI GCNGC 2 cut(s) 178, 188
Bme1390I CCNGG 5 cut(s) 313, 314, 400, 645, 1021
Bme18I GGWCC 2 cut(s) 217, 402
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 3 cut(s) 217, 315, 402
BmiI GGNNCC 2 cut(s) 310, 642
BmrFI CCNGG 5 cut(s) 313, 314, 400, 645, 1021
BmsI GCATC 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 666
BpuMI CCSGG 2 cut(s) 313, 314
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 9 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020, 1060
Bsc4I CCNNNNNNNGG 1 cut(s) 1061
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 3 cut(s) 220, 409, 851
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 400, 645, 1021
BseDI CCNNGG 9 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020, 1060
BseGI GGATG 2 cut(s) 945, 1030
BseLI CCNNNNNNNGG 1 cut(s) 1061
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 3 cut(s) 220, 409, 851
BseRI GAGGAG 6 cut(s) 14, 112, 115, 118, 121, 124
BseX3I CGGCCG 1 cut(s) 355
BseYI CCCAGC 3 cut(s) 611, 635, 737
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 5 cut(s) 236, 284, 317, 357, 543
BsiEI CGRYCG 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 2 cut(s) 313, 318
BslI CCNNNNNNNGG 1 cut(s) 1061
BsnI GGCC 5 cut(s) 236, 284, 317, 357, 543
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 313, 744
Bsp143I GATC 1 cut(s) 720
BspACI CCGC 4 cut(s) 105, 177, 186, 189
BspANI GGCC 5 cut(s) 236, 284, 317, 357, 543
BspCNI CTCAG 1 cut(s) 775
BspLI GGNNCC 2 cut(s) 310, 642
BspQI GCTCTTC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 3 cut(s) 220, 409, 851
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 9 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020, 1060
BssMI GATC 1 cut(s) 720
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 226
BssT1I CCWWGG 5 cut(s) 237, 418, 544, 697, 1060
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 3 cut(s) 400, 645, 1021
Bst4CI ACNGT 1 cut(s) 772
Bst6I CTCTTC 3 cut(s) 121, 194, 449
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 762
BstEII GGTNACC 1 cut(s) 394
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 2 cut(s) 945, 1030
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 1 cut(s) 723
BstMBI GATC 1 cut(s) 720
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 2 cut(s) 308, 708
BstNI CCWGG 3 cut(s) 400, 645, 1021
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 5 cut(s) 311, 312, 398, 643, 1019
BstSFI CTRYAG 2 cut(s) 597, 667
BstZI CGGCCG 1 cut(s) 355
BsuRI GGCC 5 cut(s) 236, 284, 317, 357, 543
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 945, 1030
Cac8I GCNNGC 1 cut(s) 319
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 217, 315, 402
Cfr9I CCCGGG 1 cut(s) 312
CseI GACGC 1 cut(s) 310
CsiI ACCWGGT 1 cut(s) 398
Csp6I GTAC 1 cut(s) 412
CviAII CATG 2 cut(s) 292, 448
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 1 cut(s) 762
DpnI GATC 1 cut(s) 722
DpnII GATC 1 cut(s) 720
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 355
EagI CGGCCG 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 121, 194, 449
EarI CTCTTC 3 cut(s) 121, 194, 449
EclXI CGGCCG 1 cut(s) 355
Eco130I CCWWGG 5 cut(s) 237, 418, 544, 697, 1060
Eco24I GRGCYC 2 cut(s) 313, 744
Eco32I GATATC 3 cut(s) 370, 862, 976
Eco47I GGWCC 2 cut(s) 217, 402
Eco52I CGGCCG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO109I RGGNCCY 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 398, 643, 1019
EcoRV GATATC 3 cut(s) 370, 862, 976
EcoT14I CCWWGG 5 cut(s) 237, 418, 544, 697, 1060
EcoT38I GRGCYC 2 cut(s) 313, 744
ErhI CCWWGG 5 cut(s) 237, 418, 544, 697, 1060
FaeI CATG 2 cut(s) 295, 451
FatI CATG 2 cut(s) 291, 447
FauNDI CATATG 1 cut(s) 468
FbaI TGATCA 1 cut(s) 720
Fnu4HI GCNGC 2 cut(s) 177, 187
FokI GGATG 2 cut(s) 952, 1037
FriOI GRGCYC 2 cut(s) 313, 744
Fsp4HI GCNGC 2 cut(s) 177, 187
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 438
GluI GCNGC 2 cut(s) 177, 187
GsaI CCCAGC 3 cut(s) 615, 639, 741
HaeIII GGCC 5 cut(s) 236, 284, 317, 357, 543
HapII CCGG 2 cut(s) 313, 318
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 2 cut(s) 295, 451
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 2 cut(s) 991, 1045
HindII GTYRAC 2 cut(s) 991, 1045
HinfI GANTC 8 cut(s) 4, 242, 362, 517, 623, 805, 854, 1069
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 2 cut(s) 313, 318
HphI GGTGA 3 cut(s) 56, 203, 388
Hpy166II GTNNAC 2 cut(s) 991, 1045
Hpy188I TCNGA 7 cut(s) 217, 308, 367, 561, 628, 765, 859
Hpy188III TCNNGA 3 cut(s) 343, 691, 968
Hpy8I GTNNAC 2 cut(s) 991, 1045
Hpy99I CGWCG 5 cut(s) 170, 308, 326, 329, 344
HpyAV CCTTC 3 cut(s) 385, 415, 930
HpyCH4III ACNGT 1 cut(s) 772
HpyCH4V TGCA 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 708
HpyF3I CTNAG 1 cut(s) 762
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 2 cut(s) 295, 451
HspAI GCGC 1 cut(s) 437
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 1 cut(s) 720
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 3 cut(s) 107, 308, 922
LweI GCATC 1 cut(s) 724
MabI ACCWGGT 1 cut(s) 398
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 3 cut(s) 359, 394, 886
MalI GATC 1 cut(s) 722
MboI GATC 1 cut(s) 720
MboII GAAGA 7 cut(s) 31, 49, 79, 108, 211, 436, 487
MhlI GDGCHC 2 cut(s) 313, 744
MluCI AATT 6 cut(s) 68, 347, 617, 650, 783, 909
MlyI GAGTC 3 cut(s) 236, 356, 1063
MmeI TCCRAC 2 cut(s) 286, 772
MroNI GCCGGC 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 676
MseI TTAA 3 cut(s) 923, 981, 990
MslI CAYNNNNRTG 1 cut(s) 764
MspI CCGG 2 cut(s) 313, 318
MspR9I CCNGG 5 cut(s) 313, 314, 400, 645, 1021
MvaI CCWGG 3 cut(s) 400, 645, 1021
MwoI GCNNNNNNNGC 2 cut(s) 308, 708
NaeI GCCGGC 1 cut(s) 319
NciI CCSGG 2 cut(s) 313, 314
NdeI CATATG 1 cut(s) 468
NdeII GATC 1 cut(s) 720
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 2 cut(s) 295, 451
NlaIV GGNNCC 2 cut(s) 310, 642
NmeAIII GCCGAG 2 cut(s) 252, 333
NmuCI GTSAC 2 cut(s) 359, 394
PciSI GCTCTTC 1 cut(s) 194
PdiI GCCGGC 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 4 cut(s) 517, 623, 805, 854
PkrI GCNGC 2 cut(s) 178, 188
PleI GAGTC 3 cut(s) 236, 356, 1063
PpsI GAGTC 3 cut(s) 236, 356, 1063
PpuMI RGGWCCY 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 3 cut(s) 398, 643, 1019
PspEI GGTNACC 1 cut(s) 394
PspFI CCCAGC 3 cut(s) 611, 635, 737
PspGI CCWGG 3 cut(s) 398, 643, 1019
PspN4I GGNNCC 2 cut(s) 310, 642
PspPI GGNCC 3 cut(s) 217, 315, 402
PspPPI RGGWCCY 1 cut(s) 402
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 764
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 3 cut(s) 923, 981, 990
SatI GCNGC 2 cut(s) 177, 187
Sau3AI GATC 1 cut(s) 720
Sau96I GGNCC 3 cut(s) 217, 315, 402
SchI GAGTC 3 cut(s) 236, 356, 1063
ScrFI CCNGG 5 cut(s) 313, 314, 400, 645, 1021
SduI GDGCHC 2 cut(s) 313, 744
SexAI ACCWGGT 1 cut(s) 398
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 2 cut(s) 597, 667
SinI GGWCC 2 cut(s) 217, 402
SmaI CCCGGG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
SrfI GCCCGGGC 1 cut(s) 314
Sse9I AATT 6 cut(s) 68, 347, 617, 650, 783, 909
SsiI CCGC 4 cut(s) 105, 177, 186, 189
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 5 cut(s) 311, 312, 398, 643, 1019
StyI CCWWGG 5 cut(s) 237, 418, 544, 697, 1060
TaaI ACNGT 1 cut(s) 772
TaqI TCGA 1 cut(s) 327
TasI AATT 6 cut(s) 68, 347, 617, 650, 783, 909
TauI GCSGC 2 cut(s) 179, 189
TfiI GAWTC 4 cut(s) 517, 623, 805, 854
Tru1I TTAA 3 cut(s) 923, 981, 990
Tru9I TTAA 3 cut(s) 923, 981, 990
TseFI GTSAC 2 cut(s) 359, 394
Tsp45I GTSAC 2 cut(s) 359, 394
TspDTI ATGAA 4 cut(s) 308, 436, 818, 1041
TspGWI ACGGA 2 cut(s) 127, 917
TspMI CCCGGG 1 cut(s) 312
VpaK11BI GGWCC 2 cut(s) 217, 402
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 2 cut(s) 347, 909
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmaI CCCGGG 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.