RLG00000019201

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010955
Physical Location & Seq
Forward (+)
48196373 .. 48203259
6887 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019201

Sequence Viewer

Length: 1320 bp
ATGACTCTCTGCAACTCATTTCTCCTCAAAACCCCAATCTTCCCTCACTCCCATTTCTTCACTCACCAATTTCCCACCAACAAACCCATCTTCTCCCTCTCCTGCTCCGCAAACCACTCTCCTTCTTCTTCCTCCGTGCCTGACCCCAAAACCAACGCCAGCGACGACGGCAACGCCACCAATGGCGGGGGTGAGCCGAAGAACAAGAGGCTTTCGGACCAGTCCTCGTGGGAGGCCAAGGACTCTGTGGGCGAGGACTACTTATACAGGCTCGGCAAAGAGGCCGACAACATGAACATCGCCGTCGGAGCCCGGGCCGGCGTCGTCGATGACCTCTTCGTCGGCAAGTTCCTCGGCCGTGACTCCGATATCGTCTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTCCAAGGCGATTATTACATTGCGCCTCTCTTCATGGATAAAGTCGTATGCCATATGGCGAAGAACTACCTTGCTCATATTCTCAATACGAAAGTTCCTTTGATTCTAGGTATTTGGGGAGGAAAAGGCCAAGGAAAGTCATTTCAGACTGAACTGGTATTTCAAGCTATGGGAGTTGAACCTGTAGTTATGTCTGCTGGGGAATTAGAATCAGAAAGAGCTGGGGAACCAGGGAAATTGATACGGGAACGCTATAGAACAGCTTCTCAAGTTGTCCAGAACCAAGGCAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGACTCGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATTGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGACTGGCGAGAATCAGATATCACAAACAGAGTTCCTATCATTGTTACAGGAAATGATTTTTCAAAAATTTATGCTCCTTTAATCCGTGATGGAAGGATGGAAAAGTTTTATTGGCAACCTAACCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTTGACACTTTTCCTAACCAAGCACTGGATTTCTATGGAGCTCTACGGTCTCGAACATATGATCGGTCAATTTCTAAGTGGATTGATGATATTGGCGGTGTTGAAAGTCTTGGAAACAAACTTGTGAAGCGAAGAAAGGATGACAATCTTCCTGTATATACTCCTCCAAAGCAAACGATGGAGGCTTTGCTTGAATCCGGGTATTCTCTGCTGAAAGAACAGCAGTTGATAATGGAGACCAAACTTTCAAAGGAATACATGAAGAACATGGATGATTAG

Protein Analysis

440

Amino Acids

49.4

Weight (kDa)

6.15

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 176 - 320 9e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 323 - 421 1.9e-34 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 1066
AciI CCGC 3 cut(s) 108, 186, 1137
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 1 cut(s) 909
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 186, 1066
AgsI TTSAA 6 cut(s) 578, 593, 906, 1145, 1235, 1290
AjnI CCWGG 3 cut(s) 398, 643, 1019
AluBI AGCT 5 cut(s) 581, 635, 677, 711, 1082
AluI AGCT 5 cut(s) 581, 635, 677, 711, 1082
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 2 cut(s) 1095, 1271
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 5 cut(s) 234, 282, 315, 355, 541
ApoI RAATTY 1 cut(s) 909
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 217, 315, 402
AsuC2I CCSGG 3 cut(s) 313, 314, 1240
AsuHPI GGTGA 3 cut(s) 56, 203, 388
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 217, 402
BanII GRGCYC 2 cut(s) 313, 1084
BauI CACGAG 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 1084
BccI CCATC 5 cut(s) 95, 926, 934, 1004, 1213
BceAI ACGGC 3 cut(s) 184, 287, 342
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 3 cut(s) 400, 645, 1021
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 3 cut(s) 313, 314, 1240
BcoDI GTCTC 2 cut(s) 1095, 1271
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 597, 667
Bme1390I CCNGG 6 cut(s) 313, 314, 400, 645, 1021, 1240
Bme18I GGWCC 2 cut(s) 217, 402
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 3 cut(s) 217, 315, 402
BmiI GGNNCC 2 cut(s) 310, 642
BmrFI CCNGG 6 cut(s) 313, 314, 400, 645, 1021, 1240
BmsI GCATC 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 666
BpuMI CCSGG 3 cut(s) 313, 314, 1240
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 2 cut(s) 1095, 1271
BsaJI CCNNGG 8 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 1066
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 5 cut(s) 220, 409, 573, 851, 1071
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 1185
BseBI CCWGG 3 cut(s) 400, 645, 1021
BseDI CCNNGG 8 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020
BseGI GGATG 4 cut(s) 945, 1030, 1186, 1318
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 2 cut(s) 186, 1066
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 5 cut(s) 220, 409, 573, 851, 1071
BseRI GAGGAG 2 cut(s) 14, 1194
BseX3I CGGCCG 1 cut(s) 355
BseYI CCCAGC 2 cut(s) 611, 635
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 5 cut(s) 236, 284, 317, 357, 543
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 313, 318, 1239
BslI CCNNNNNNNGG 2 cut(s) 186, 1066
BsmAI GTCTC 2 cut(s) 1095, 1271
BsnI GGCC 5 cut(s) 236, 284, 317, 357, 543
Bso31I GGTCTC 2 cut(s) 1095, 1271
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 313, 1084
Bsp143I GATC 2 cut(s) 720, 1102
BspACI CCGC 3 cut(s) 108, 186, 1137
BspANI GGCC 5 cut(s) 236, 284, 317, 357, 543
BspCNI CTCAG 1 cut(s) 775
BspLI GGNNCC 2 cut(s) 310, 642
BspTNI GGTCTC 2 cut(s) 1095, 1271
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 5 cut(s) 220, 409, 573, 851, 1071
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 8 cut(s) 237, 312, 352, 418, 544, 644, 697, 1020
BssMI GATC 2 cut(s) 720, 1102
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 226
BssT1I CCWWGG 4 cut(s) 237, 418, 544, 697
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 3 cut(s) 400, 645, 1021
Bst4CI ACNGT 3 cut(s) 772, 968, 1089
Bst6I CTCTTC 2 cut(s) 341, 449
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 160, 319
BstDEI CTNAG 2 cut(s) 762, 1116
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 4 cut(s) 945, 1030, 1186, 1318
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 2 cut(s) 723, 1105
BstMAI GTCTC 2 cut(s) 1095, 1271
BstMBI GATC 2 cut(s) 720, 1102
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 3 cut(s) 168, 308, 708
BstNI CCWGG 3 cut(s) 400, 645, 1021
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 6 cut(s) 311, 312, 398, 643, 1019, 1238
BstSFI CTRYAG 2 cut(s) 597, 667
BstZI CGGCCG 1 cut(s) 355
BsuRI GGCC 5 cut(s) 236, 284, 317, 357, 543
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 4 cut(s) 945, 1030, 1186, 1318
BtsIMutI CAGTG 1 cut(s) 1064
Cac8I GCNNGC 2 cut(s) 160, 319
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 217, 315, 402
Cfr9I CCCGGG 1 cut(s) 312
CseI GACGC 1 cut(s) 310
CsiI ACCWGGT 1 cut(s) 398
Csp6I GTAC 1 cut(s) 412
CviAII CATG 4 cut(s) 292, 448, 1300, 1309
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 762, 1116
DpnI GATC 2 cut(s) 722, 1104
DpnII GATC 2 cut(s) 720, 1102
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 355
EagI CGGCCG 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 341, 449
EarI CTCTTC 2 cut(s) 341, 449
Ecl136II GAGCTC 1 cut(s) 1082
EclXI CGGCCG 1 cut(s) 355
Eco130I CCWWGG 4 cut(s) 237, 418, 544, 697
Eco24I GRGCYC 2 cut(s) 313, 1084
Eco31I GGTCTC 2 cut(s) 1095, 1271
Eco32I GATATC 3 cut(s) 370, 862, 976
Eco47I GGWCC 2 cut(s) 217, 402
Eco52I CGGCCG 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 1082
Eco88I CYCGRG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 1082
EcoO109I RGGNCCY 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 398, 643, 1019
EcoRV GATATC 3 cut(s) 370, 862, 976
EcoT14I CCWWGG 4 cut(s) 237, 418, 544, 697
EcoT38I GRGCYC 2 cut(s) 313, 1084
ErhI CCWWGG 4 cut(s) 237, 418, 544, 697
FaeI CATG 4 cut(s) 295, 451, 1303, 1312
FatI CATG 4 cut(s) 291, 447, 1299, 1308
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 2 cut(s) 468, 1099
FbaI TGATCA 1 cut(s) 720
FokI GGATG 3 cut(s) 952, 1037, 1193
FriOI GRGCYC 2 cut(s) 313, 1084
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 438
GsaI CCCAGC 2 cut(s) 615, 639
HaeIII GGCC 5 cut(s) 236, 284, 317, 357, 543
HapII CCGG 3 cut(s) 313, 318, 1239
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 4 cut(s) 295, 451, 1303, 1312
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 2 cut(s) 991, 1045
HindII GTYRAC 2 cut(s) 991, 1045
HinfI GANTC 9 cut(s) 4, 242, 362, 517, 623, 741, 805, 854, 1235
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 3 cut(s) 313, 318, 1239
HphI GGTGA 3 cut(s) 56, 203, 388
Hpy166II GTNNAC 2 cut(s) 991, 1045
Hpy188I TCNGA 7 cut(s) 217, 308, 367, 561, 628, 765, 859
Hpy188III TCNNGA 2 cut(s) 691, 1092
Hpy8I GTNNAC 2 cut(s) 991, 1045
Hpy99I CGWCG 6 cut(s) 167, 170, 308, 326, 329, 344
HpyAV CCTTC 4 cut(s) 132, 385, 415, 930
HpyCH4III ACNGT 3 cut(s) 772, 968, 1089
HpyCH4V TGCA 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 308, 708
HpyF3I CTNAG 2 cut(s) 762, 1116
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 4 cut(s) 295, 451, 1303, 1312
HspAI GCGC 1 cut(s) 437
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 2 cut(s) 720, 1102
LmnI GCTCC 4 cut(s) 110, 308, 922, 1079
LweI GCATC 1 cut(s) 724
MabI ACCWGGT 1 cut(s) 398
MaeI CTAG 1 cut(s) 521
MaeIII GTNAC 3 cut(s) 359, 394, 886
MalI GATC 2 cut(s) 722, 1104
MboI GATC 2 cut(s) 720, 1102
MhlI GDGCHC 2 cut(s) 313, 1084
MluCI AATT 6 cut(s) 68, 617, 650, 783, 909, 1110
MlyI GAGTC 3 cut(s) 236, 356, 735
MmeI TCCRAC 2 cut(s) 286, 772
MroNI GCCGGC 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 676
MseI TTAA 3 cut(s) 923, 981, 990
MslI CAYNNNNRTG 1 cut(s) 764
MspI CCGG 3 cut(s) 313, 318, 1239
MspR9I CCNGG 6 cut(s) 313, 314, 400, 645, 1021, 1240
MvaI CCWGG 3 cut(s) 400, 645, 1021
MwoI GCNNNNNNNGC 3 cut(s) 168, 308, 708
NaeI GCCGGC 1 cut(s) 319
NciI CCSGG 3 cut(s) 313, 314, 1240
NdeI CATATG 2 cut(s) 468, 1099
NdeII GATC 2 cut(s) 720, 1102
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 4 cut(s) 295, 451, 1303, 1312
NlaIV GGNNCC 2 cut(s) 310, 642
NmeAIII GCCGAG 2 cut(s) 252, 333
NmuCI GTSAC 2 cut(s) 359, 394
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 5 cut(s) 517, 623, 805, 854, 1235
PflMI CCANNNNNTGG 1 cut(s) 1066
PleI GAGTC 3 cut(s) 236, 356, 735
PpsI GAGTC 3 cut(s) 236, 356, 735
PpuMI RGGWCCY 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 1084
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 3 cut(s) 398, 643, 1019
PspEI GGTNACC 1 cut(s) 394
PspFI CCCAGC 2 cut(s) 611, 635
PspGI CCWGG 3 cut(s) 398, 643, 1019
PspN4I GGNNCC 2 cut(s) 310, 642
PspPI GGNCC 3 cut(s) 217, 315, 402
PspPPI RGGWCCY 1 cut(s) 402
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 1 cut(s) 1084
SaqAI TTAA 3 cut(s) 923, 981, 990
Sau3AI GATC 2 cut(s) 720, 1102
Sau96I GGNCC 3 cut(s) 217, 315, 402
SchI GAGTC 3 cut(s) 236, 356, 735
ScrFI CCNGG 6 cut(s) 313, 314, 400, 645, 1021, 1240
SduI GDGCHC 2 cut(s) 313, 1084
SexAI ACCWGGT 1 cut(s) 398
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 2 cut(s) 597, 667
SinI GGWCC 2 cut(s) 217, 402
SmaI CCCGGG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
SrfI GCCCGGGC 1 cut(s) 314
Sse9I AATT 6 cut(s) 68, 617, 650, 783, 909, 1110
SsiI CCGC 3 cut(s) 108, 186, 1137
SspMI CTAG 1 cut(s) 521
SstI GAGCTC 1 cut(s) 1084
StyD4I CCNGG 6 cut(s) 311, 312, 398, 643, 1019, 1238
StyI CCWWGG 4 cut(s) 237, 418, 544, 697
TaaI ACNGT 3 cut(s) 772, 968, 1089
TaqI TCGA 2 cut(s) 327, 1093
TaqII GACCGA 1 cut(s) 1095
TasI AATT 6 cut(s) 68, 617, 650, 783, 909, 1110
TfiI GAWTC 5 cut(s) 517, 623, 805, 854, 1235
Tru1I TTAA 3 cut(s) 923, 981, 990
Tru9I TTAA 3 cut(s) 923, 981, 990
TscAI CASTG 1 cut(s) 1071
TseFI GTSAC 2 cut(s) 359, 394
Tsp45I GTSAC 2 cut(s) 359, 394
TspDTI ATGAA 5 cut(s) 308, 436, 818, 1041, 1316
TspGWI ACGGA 2 cut(s) 124, 917
TspMI CCCGGG 1 cut(s) 312
TspRI CASTG 1 cut(s) 1071
Van91I CCANNNNNTGG 1 cut(s) 1066
VpaK11BI GGWCC 2 cut(s) 217, 402
XapI RAATTY 1 cut(s) 909
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmaI CCCGGG 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.