Rh2AG351100

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010955
Physical Location & Seq
Forward (+)
51641757 .. 51648757
7001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG351100.1

Sequence Viewer

Length: 1353 bp
ATGACTCTCTGCAACTCATTTCTCCTCAAAACCCCAATCTTCCCTCACTCCCATTTCTTCACTCACCAATTTCCCACCAAACCCATCTTCTCCCTTTCCTGCTCCGCAACCCCCTCTTCCTCCTCCTCCTCCGTGCCTGACCCCAAAACCAACGCCAACGACGGCAACGCCGCCAATGGCGGCGGTGAGGCGAAGAGCAAGAGGCTTTCGGACCAGTCCTCGTGGGAGGCCAAGGACTCTGTGGGCGAGGACTACTTATACAGGCTCGGCAAAGAGGCCGACAACATGAACATCGCCGTCGGAGCCCGGGCCGGCGTTGTCGATGACCTTTTCGTCGGGAAATTCCTCGGCCGTGACTCCGATATCGTCTTTGATTATCGCCAGAAGGTCACCAGGTCCTTCCAGTACCTGCAAGGCGATTATTACATTGCGCCTCTCTTCATGGATAAAGTCGTATGCCATATGGCGAAGAACTACCTTGCTCATATTCTCAATACGAAAGTTCCTTTGATTCTAGGTATTTGGGGAGGAAAAGGCCAAGGAAAGTCATTTCAGACTGAACTTGTATTTCAAGCTATGGGAGTTGAACCTGTAGTTATGTCTGCTGGGGAATTAGAATCAGAAAGAGCTGGGGAACCAGGGAAATTGATACGGGAACGCTATAGAACAGCTTCTCAAGTTGTCCAGAACCAAGGCAAGATGAGCTGTTTGATGATCAATGACATTGATGCTGGGCTCGGTAGATTTGGAAACACTCAGATGACAGTCAATAATCAAATCGTTGTTGGAACTTTGATGAATCTATGTGATAATCCTACAAGAGTTAGTATTGGACAAGAGTGGCGAGAATCAGATATCACAAACAGAGTTCCTATCATTGTTACAGGAAATGATTTTTCAAAAATTTATGCTCCCTTAATCCGTGATGGAAGGATGGAAAAGTTTTATTGGCAACCTAACCGTGAGGATATCATTAACATTGTTAACAGAATGTATGAGAAAGATGGCATATCCAGGGATGAAGTTGTAAGTATAGTTGACACTTTTCCTAATCAAGCACTGGATTTCTATGGAGCTCTACGTTCTCGAACATATGATCGGTCAATTTCTAAGTGGATTGATGATATTGGCGGTGTTGAAAGTCTTGGAAACAAACTTGTGAAGCGGAGAAAGGATGACAATCTTCCTGTATATACTCCTCCAAAGGTATTGTGGTCCATCATAAAAAAGTGTTTTATTTTCGAAGCCTGCTATCCATATAACCAGTTTTTGCATTCAGCGAACTGGGTTCATCTCCTGTTAATATTTGAGAGCCAATTAAATAAATTGAACTACAGTACAACTAGTTGGTAA

Protein Analysis

450

Amino Acids

50.74

Weight (kDa)

8.03

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 174 - 318 9.5e-15 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 321 - 403 4.2e-28 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73110 AT1G73110
fragaria_vesca FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970 FvH4_6g27970
malus_domestica MD09G1230800.v1.1
prunus_persica Prupe.3G107000_v2.0.a1 Prupe.3G107000_v2.0.a1
pyrus_communis pycom09g14970
rosa_chinensis RchiOBHm_Chr2g0131521
rosa_laevigata RLG00000019201
rosa_multiflora Rmu_sc0013257.1_g000001
rosa_roxburghii Rroxscaffold_2G00112760
rosa_samantha Rh2AG351100 Rh2BG357700 Rh2CG336700 Rh2DG375500
rosa_wichuraiana Rw2G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 417
AciI CCGC 6 cut(s) 105, 171, 180, 183, 1131, 1165
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 341, 903
AfaI GTAC 2 cut(s) 407, 1339
AgsI TTSAA 5 cut(s) 572, 587, 900, 1139, 1330
AhlI ACTAGT 1 cut(s) 1343
AjnI CCWGG 3 cut(s) 392, 637, 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 5 cut(s) 575, 629, 671, 705, 1076
AluI AGCT 5 cut(s) 575, 629, 671, 705, 1076
Alw21I GWGCWC 1 cut(s) 1078
AlwNI CAGNNNCTG 1 cut(s) 409
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 5 cut(s) 228, 276, 309, 349, 535
ApoI RAATTY 2 cut(s) 341, 903
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 4 cut(s) 211, 309, 396, 1215
AsuC2I CCSGG 2 cut(s) 307, 308
AsuHPI GGTGA 3 cut(s) 56, 197, 382
AsuII TTCGAA 1 cut(s) 1242
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 3 cut(s) 211, 396, 1215
BanII GRGCYC 3 cut(s) 307, 738, 1078
BauI CACGAG 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 1078
BccI CCATC 5 cut(s) 92, 920, 928, 998, 1226
BceAI ACGGC 3 cut(s) 178, 281, 336
BciT130I CCWGG 3 cut(s) 394, 639, 1015
BclI TGATCA 1 cut(s) 714
BcnI CCSGG 2 cut(s) 307, 308
BcuI ACTAGT 1 cut(s) 1343
BfaI CTAG 2 cut(s) 515, 1344
BfmI CTRYAG 3 cut(s) 591, 661, 1333
BfuAI ACCTGC 1 cut(s) 417
BisI GCNGC 2 cut(s) 171, 181
BlsI GCNGC 2 cut(s) 172, 182
Bme1390I CCNGG 5 cut(s) 307, 308, 394, 639, 1015
Bme18I GGWCC 3 cut(s) 211, 396, 1215
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 4 cut(s) 211, 309, 396, 1215
BmiI GGNNCC 2 cut(s) 304, 636
BmrFI CCNGG 5 cut(s) 307, 308, 394, 639, 1015
BmrI ACTGGG 1 cut(s) 1294
BmsI GCATC 1 cut(s) 718
BmuI ACTGGG 1 cut(s) 1294
Bpu14I TTCGAA 1 cut(s) 1242
BpuEI CTTGAG 1 cut(s) 660
BpuMI CCSGG 2 cut(s) 307, 308
BsaBI GATNNNNATC 1 cut(s) 1179
BsaJI CCNNGG 7 cut(s) 231, 306, 346, 538, 638, 691, 1014
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 5 cut(s) 214, 403, 1065, 1264, 1289
Bse3DI GCAATG 1 cut(s) 426
Bse8I GATNNNNATC 1 cut(s) 1179
BseBI CCWGG 3 cut(s) 394, 639, 1015
BseDI CCNNGG 7 cut(s) 231, 306, 346, 538, 638, 691, 1014
BseGI GGATG 3 cut(s) 939, 1024, 1180
BseJI GATNNNNATC 1 cut(s) 1179
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 5 cut(s) 214, 403, 1065, 1264, 1289
BseRI GAGGAG 5 cut(s) 14, 112, 115, 118, 1188
BseX3I CGGCCG 1 cut(s) 349
BseYI CCCAGC 3 cut(s) 605, 629, 731
Bsh1285I CGRYCG 1 cut(s) 352
BshFI GGCC 5 cut(s) 230, 278, 311, 351, 537
BsiEI CGRYCG 1 cut(s) 352
BsiHKAI GWGCWC 1 cut(s) 1078
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 2 cut(s) 307, 312
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 5 cut(s) 230, 278, 311, 351, 537
BsoBI CYCGRG 1 cut(s) 306
Bsp119I TTCGAA 1 cut(s) 1242
Bsp1286I GDGCHC 3 cut(s) 307, 738, 1078
Bsp143I GATC 2 cut(s) 714, 1096
BspACI CCGC 6 cut(s) 105, 171, 180, 183, 1131, 1165
BspANI GGCC 5 cut(s) 230, 278, 311, 351, 537
BspCNI CTCAG 1 cut(s) 769
BspLI GGNNCC 2 cut(s) 304, 636
BspMI ACCTGC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 188
BspT104I TTCGAA 1 cut(s) 1242
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 5 cut(s) 214, 403, 1065, 1264, 1289
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 7 cut(s) 231, 306, 346, 538, 638, 691, 1014
BssMI GATC 2 cut(s) 714, 1096
BssSI CACGAG 1 cut(s) 220
BssT1I CCWWGG 3 cut(s) 231, 538, 691
Bst2BI CACGAG 1 cut(s) 220
Bst2UI CCWGG 3 cut(s) 394, 639, 1015
Bst4CI ACNGT 3 cut(s) 766, 962, 1337
Bst6I CTCTTC 3 cut(s) 121, 188, 443
BstBI TTCGAA 1 cut(s) 1242
BstC8I GCNNGC 2 cut(s) 313, 1249
BstDEI CTNAG 2 cut(s) 756, 1110
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 3 cut(s) 939, 1024, 1180
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 717, 1099
BstMBI GATC 2 cut(s) 714, 1096
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 2 cut(s) 302, 702
BstNI CCWGG 3 cut(s) 394, 639, 1015
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 5 cut(s) 305, 306, 392, 637, 1013
BstSFI CTRYAG 3 cut(s) 591, 661, 1333
BstZI CGGCCG 1 cut(s) 349
BsuRI GGCC 5 cut(s) 230, 278, 311, 351, 537
BtgZI GCGATG 1 cut(s) 277
BtsCI GGATG 3 cut(s) 939, 1024, 1180
BtsIMutI CAGTG 1 cut(s) 1058
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 313, 1249
CaiI CAGNNNCTG 1 cut(s) 409
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 4 cut(s) 211, 309, 396, 1215
Cfr9I CCCGGG 1 cut(s) 306
CsiI ACCWGGT 1 cut(s) 392
Csp6I GTAC 2 cut(s) 406, 1338
CviAII CATG 2 cut(s) 286, 442
CviQI GTAC 2 cut(s) 406, 1338
DdeI CTNAG 2 cut(s) 756, 1110
DpnI GATC 2 cut(s) 716, 1098
DpnII GATC 2 cut(s) 714, 1096
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 349
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 3 cut(s) 121, 188, 443
EarI CTCTTC 3 cut(s) 121, 188, 443
Ecl136II GAGCTC 1 cut(s) 1076
EclXI CGGCCG 1 cut(s) 349
Eco130I CCWWGG 3 cut(s) 231, 538, 691
Eco24I GRGCYC 3 cut(s) 307, 738, 1078
Eco32I GATATC 3 cut(s) 364, 856, 970
Eco47I GGWCC 3 cut(s) 211, 396, 1215
Eco52I CGGCCG 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 1076
Eco88I CYCGRG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 1076
EcoO109I RGGNCCY 1 cut(s) 396
EcoO65I GGTNACC 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 392, 637, 1013
EcoRV GATATC 3 cut(s) 364, 856, 970
EcoT14I CCWWGG 3 cut(s) 231, 538, 691
EcoT38I GRGCYC 3 cut(s) 307, 738, 1078
ErhI CCWWGG 3 cut(s) 231, 538, 691
FaeI CATG 2 cut(s) 289, 445
FatI CATG 2 cut(s) 285, 441
FauNDI CATATG 2 cut(s) 462, 1093
FbaI TGATCA 1 cut(s) 714
Fnu4HI GCNGC 2 cut(s) 171, 181
FokI GGATG 3 cut(s) 946, 1031, 1187
FriOI GRGCYC 3 cut(s) 307, 738, 1078
Fsp4HI GCNGC 2 cut(s) 171, 181
FspBI CTAG 2 cut(s) 515, 1344
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 171, 181
GsaI CCCAGC 3 cut(s) 609, 633, 735
HaeIII GGCC 5 cut(s) 230, 278, 311, 351, 537
HapII CCGG 2 cut(s) 307, 312
HhaI GCGC 1 cut(s) 433
Hin1II CATG 2 cut(s) 289, 445
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 2 cut(s) 985, 1039
HindII GTYRAC 2 cut(s) 985, 1039
HinfI GANTC 7 cut(s) 4, 236, 356, 511, 617, 799, 848
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 2 cut(s) 307, 312
HphI GGTGA 3 cut(s) 56, 197, 382
Hpy166II GTNNAC 2 cut(s) 985, 1039
Hpy188I TCNGA 7 cut(s) 211, 302, 361, 555, 622, 759, 853
Hpy188III TCNNGA 3 cut(s) 337, 685, 1086
Hpy8I GTNNAC 2 cut(s) 985, 1039
Hpy99I CGWCG 3 cut(s) 164, 302, 338
HpyAV CCTTC 3 cut(s) 379, 409, 924
HpyCH4III ACNGT 3 cut(s) 766, 962, 1337
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 3 cut(s) 12, 412, 1273
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 702
HpyF3I CTNAG 2 cut(s) 756, 1110
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 2 cut(s) 289, 445
HspAI GCGC 1 cut(s) 431
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 714
KspAI GTTAAC 1 cut(s) 985
Kzo9I GATC 2 cut(s) 714, 1096
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 4 cut(s) 107, 302, 916, 1073
LweI GCATC 1 cut(s) 718
MabI ACCWGGT 1 cut(s) 392
MaeI CTAG 2 cut(s) 515, 1344
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 3 cut(s) 353, 388, 880
MalI GATC 2 cut(s) 716, 1098
MboI GATC 2 cut(s) 714, 1096
MboII GAAGA 8 cut(s) 31, 49, 79, 108, 205, 430, 481, 1175
MhlI GDGCHC 3 cut(s) 307, 738, 1078
MluCI AATT 8 cut(s) 68, 341, 611, 644, 903, 1104, 1316, 1325
MlyI GAGTC 2 cut(s) 230, 350
MmeI TCCRAC 2 cut(s) 280, 766
MroNI GCCGGC 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 670
MseI TTAA 5 cut(s) 917, 975, 984, 1301, 1319
MslI CAYNNNNRTG 1 cut(s) 758
MspI CCGG 2 cut(s) 307, 312
MspR9I CCNGG 5 cut(s) 307, 308, 394, 639, 1015
Mva1269I GAATGC 1 cut(s) 1273
MvaI CCWGG 3 cut(s) 394, 639, 1015
MwoI GCNNNNNNNGC 2 cut(s) 302, 702
NaeI GCCGGC 1 cut(s) 313
NciI CCSGG 2 cut(s) 307, 308
NdeI CATATG 2 cut(s) 462, 1093
NdeII GATC 2 cut(s) 714, 1096
NgoMIV GCCGGC 1 cut(s) 311
NlaIII CATG 2 cut(s) 289, 445
NlaIV GGNNCC 2 cut(s) 304, 636
NmeAIII GCCGAG 2 cut(s) 246, 327
NmuCI GTSAC 2 cut(s) 353, 388
NspV TTCGAA 1 cut(s) 1242
PciSI GCTCTTC 1 cut(s) 188
PctI GAATGC 1 cut(s) 1273
PdiI GCCGGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 670
PfeI GAWTC 4 cut(s) 511, 617, 799, 848
PkrI GCNGC 2 cut(s) 172, 182
PleI GAGTC 2 cut(s) 230, 350
PpsI GAGTC 2 cut(s) 230, 350
PpuMI RGGWCCY 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 1078
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 3 cut(s) 392, 637, 1013
PspEI GGTNACC 1 cut(s) 388
PspFI CCCAGC 3 cut(s) 605, 629, 731
PspGI CCWGG 3 cut(s) 392, 637, 1013
PspN4I GGNNCC 2 cut(s) 304, 636
PspPI GGNCC 4 cut(s) 211, 309, 396, 1215
PspPPI RGGWCCY 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 409
RsaI GTAC 2 cut(s) 407, 1339
RsaNI GTAC 2 cut(s) 406, 1338
RseI CAYNNNNRTG 1 cut(s) 758
SacI GAGCTC 1 cut(s) 1078
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 5 cut(s) 917, 975, 984, 1301, 1319
SatI GCNGC 2 cut(s) 171, 181
Sau3AI GATC 2 cut(s) 714, 1096
Sau96I GGNCC 4 cut(s) 211, 309, 396, 1215
SchI GAGTC 2 cut(s) 230, 350
ScrFI CCNGG 5 cut(s) 307, 308, 394, 639, 1015
SduI GDGCHC 3 cut(s) 307, 738, 1078
SexAI ACCWGGT 1 cut(s) 392
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 3 cut(s) 591, 661, 1333
SfuI TTCGAA 1 cut(s) 1242
SinI GGWCC 3 cut(s) 211, 396, 1215
SmaI CCCGGG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 1 cut(s) 675
SmoI CTYRAG 1 cut(s) 675
SpeI ACTAGT 1 cut(s) 1343
SrfI GCCCGGGC 1 cut(s) 308
Sse9I AATT 8 cut(s) 68, 341, 611, 644, 903, 1104, 1316, 1325
SsiI CCGC 6 cut(s) 105, 171, 180, 183, 1131, 1165
SspI AATATT 1 cut(s) 1305
SspMI CTAG 2 cut(s) 515, 1344
SstI GAGCTC 1 cut(s) 1078
StyD4I CCNGG 5 cut(s) 305, 306, 392, 637, 1013
StyI CCWWGG 3 cut(s) 231, 538, 691
TaaI ACNGT 3 cut(s) 766, 962, 1337
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 3 cut(s) 321, 1087, 1242
TaqII GACCGA 1 cut(s) 1089
TasI AATT 8 cut(s) 68, 341, 611, 644, 903, 1104, 1316, 1325
TatI WGTACW 1 cut(s) 1337
TauI GCSGC 2 cut(s) 173, 183
TfiI GAWTC 4 cut(s) 511, 617, 799, 848
Tru1I TTAA 5 cut(s) 917, 975, 984, 1301, 1319
Tru9I TTAA 5 cut(s) 917, 975, 984, 1301, 1319
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 2 cut(s) 353, 388
Tsp45I GTSAC 2 cut(s) 353, 388
TspDTI ATGAA 5 cut(s) 302, 430, 812, 1035, 1280
TspGWI ACGGA 2 cut(s) 121, 911
TspMI CCCGGG 1 cut(s) 306
TspRI CASTG 1 cut(s) 1065
VpaK11BI GGWCC 3 cut(s) 211, 396, 1215
XapI RAATTY 2 cut(s) 341, 903
XcmI CCANNNNNNNNNTGG 2 cut(s) 238, 1209
XmaI CCCGGG 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 2 cut(s) 515, 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.