FvH4_6g28830

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011955
Physical Location & Seq
Forward (+)
22254847 .. 22258353
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28830.t1

Sequence Viewer

Length: 672 bp
ATGGAAGTTCTTAGCTCATGGGACGCGCTTCGCAAACAGGCCAGGAAGCTTGAAGCTCAGTTGGATGAACAGATGAATTCTTATCGTAGGTTTGTCTCTGCAAAGGGTTCTGTCAAATTTGATGCTGATAGTGATCTTGAATCTGGGATAGACCGACTATTAAAGCAACTCCAACAAGTAAATATACAAATGCAAGCATGGGTATCATCAGGAGGATCAGAAATGGTTTCTCATACCTTGACTAGACATCAAGAGATTCTTCAAGATCTCACTCAGGAATTTTATCGTCTCCGCTCCAGCCTTAGAGCTAAGCAGGAGCACGCTTCATTACTTGAGGATTTTAGGGAGTTTGATAGATCTAGAGTAGACTTGGAAGCTGGTGGAGATTCTGCAGATAATGCTCTCCTAAAAGAACATGCTTCTGTTAGCCGAAGTACAGGGCAGATGGATACCGTGATCTCTCAAGCTCAAGCAACTCTGGGTGCACTTGTCCTTCAACGTTCAACGTTTGGTGGTATTAATTCAAAGCTCAGCAATATTGGCAGCCGCCTTCCAACGGTAAGTCATATCCTCTCTGCAATAAAGCGTAAAAAGTCTATGGATACCATCATTCTTTCCCTTGTCGCATCAGTGTGCACATTTCTCATATTTATCTACTGGTTGACAAAGTAA

Protein Analysis

224

Amino Acids

25.02

Weight (kDa)

8.69

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 134 - 198 2.8e-18 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15880
fragaria_vesca FvH4_6g28830
malus_domestica MD14G1001700.v1.1
prunus_persica Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1
pyrus_communis pycom12g00170 pycom49g00130
rosa_chinensis RchiOBHm_Chr2g0133301 RchiOBHm_Chr2g0133361
rosa_laevigata RLG00000019325
rosa_multiflora Rmu_sc0003874.1_g000012
rosa_roxburghii Rroxscaffold_2G00111230
rosa_rugosa Rorug02G0311200
rosa_samantha Rh2AG363700 Rh2BG369500 Rh2CG346900 Rh2DG386400
rosa_wichuraiana Rw2G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 366
AccII CGCG 1 cut(s) 26
AciI CCGC 2 cut(s) 292, 547
AclI AACGTT 2 cut(s) 499, 506
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 3 cut(s) 76, 116, 278
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 556
AgsI TTSAA 6 cut(s) 53, 140, 263, 497, 504, 525
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 7 cut(s) 15, 49, 56, 308, 377, 467, 529
AluI AGCT 7 cut(s) 15, 49, 56, 308, 377, 467, 529
Alw21I GWGCWC 3 cut(s) 321, 487, 638
Alw26I GTCTC 2 cut(s) 100, 293
Alw44I GTGCAC 2 cut(s) 483, 634
AlwI GGATC 1 cut(s) 223
AoxI GGCC 1 cut(s) 39
ApaLI GTGCAC 2 cut(s) 483, 634
ApeKI GCWGC 1 cut(s) 543
ApoI RAATTY 3 cut(s) 76, 116, 278
AseI ATTAAT 1 cut(s) 519
AspLEI GCGC 1 cut(s) 28
BaeGI GKGCMC 2 cut(s) 487, 638
Bbv12I GWGCWC 3 cut(s) 321, 487, 638
BbvI GCAGC 1 cut(s) 555
BccI CCATC 2 cut(s) 439, 614
BciT130I CCWGG 1 cut(s) 43
BciVI GTATCC 2 cut(s) 442, 595
BcoDI GTCTC 2 cut(s) 100, 293
BfaI CTAG 2 cut(s) 243, 360
BfmI CTRYAG 1 cut(s) 390
BfuI GTATCC 2 cut(s) 442, 595
BglII AGATCT 2 cut(s) 265, 356
BisI GCNGC 2 cut(s) 544, 547
BlpI GCTNAGC 2 cut(s) 309, 530
BlsI GCNGC 2 cut(s) 545, 548
Bme1390I CCNGG 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 2 cut(s) 112, 635
BpmI CTGGAG 1 cut(s) 280
Bpu1102I GCTNAGC 2 cut(s) 309, 530
BpuEI CTTGAG 3 cut(s) 353, 447, 453
BsaBI GATNNNNATC 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 662
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 556
BseMII CTCAG 3 cut(s) 71, 287, 544
BseNI ACTGG 1 cut(s) 662
BseSI GKGCMC 2 cut(s) 487, 638
BseXI GCAGC 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 41
BsiHKAI GWGCWC 3 cut(s) 321, 487, 638
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 2 cut(s) 100, 293
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 3 cut(s) 321, 487, 638
Bsp143I GATC 5 cut(s) 133, 215, 265, 356, 456
Bsp1720I GCTNAGC 2 cut(s) 309, 530
BspACI CCGC 2 cut(s) 292, 547
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 3 cut(s) 70, 286, 543
BspFNI CGCG 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 1 cut(s) 223
BsrBI CCGCTC 1 cut(s) 294
BsrI ACTGG 1 cut(s) 662
BssMI GATC 5 cut(s) 133, 215, 265, 356, 456
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 2 cut(s) 454, 559
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 195, 321
BstDEI CTNAG 6 cut(s) 11, 57, 273, 302, 309, 530
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 5 cut(s) 136, 218, 268, 359, 459
BstMAI GTCTC 2 cut(s) 100, 293
BstMBI GATC 5 cut(s) 133, 215, 265, 356, 456
BstMWI GCNNNNNNNGC 2 cut(s) 398, 540
BstNI CCWGG 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 2 cut(s) 487, 638
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 1 cut(s) 555
BstX2I RGATCY 2 cut(s) 265, 356
BstYI RGATCY 2 cut(s) 265, 356
BsuI GTATCC 2 cut(s) 442, 595
BsuRI GGCC 1 cut(s) 41
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 636
Cac8I GCNNGC 2 cut(s) 195, 321
CfoI GCGC 1 cut(s) 28
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 435
CviAII CATG 3 cut(s) 18, 198, 416
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 6 cut(s) 11, 57, 273, 302, 309, 530
DpnI GATC 5 cut(s) 135, 217, 267, 358, 458
DpnII GATC 5 cut(s) 133, 215, 265, 356, 456
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 3 cut(s) 21, 201, 419
FaiI YATR 8 cut(s) 19, 185, 199, 234, 417, 567, 599, 647
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 17, 197, 415
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 544, 547
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 2 cut(s) 544, 547
FspBI CTAG 2 cut(s) 243, 360
GlaI GCGC 1 cut(s) 27
GluI GCNGC 2 cut(s) 544, 547
GsuI CTGGAG 1 cut(s) 280
HaeIII GGCC 1 cut(s) 41
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 28
Hin1II CATG 3 cut(s) 21, 201, 419
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 3 cut(s) 140, 256, 386
Hpy166II GTNNAC 4 cut(s) 367, 485, 636, 663
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 6 cut(s) 137, 210, 251, 263, 275, 360
Hpy8I GTNNAC 4 cut(s) 367, 485, 636, 663
HpyAV CCTTC 2 cut(s) 503, 560
HpyCH4III ACNGT 2 cut(s) 454, 559
HpyCH4IV ACGT 2 cut(s) 499, 506
HpyCH4V TGCA 6 cut(s) 101, 193, 392, 485, 578, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 540
HpyF3I CTNAG 6 cut(s) 11, 57, 273, 302, 309, 530
HpySE526I ACGT 2 cut(s) 499, 506
Hsp92II CATG 3 cut(s) 21, 201, 419
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 5 cut(s) 133, 215, 265, 356, 456
LmnI GCTCC 2 cut(s) 299, 316
Lsp1109I GCAGC 1 cut(s) 555
LweI GCATC 2 cut(s) 112, 635
MaeI CTAG 2 cut(s) 243, 360
MaeII ACGT 2 cut(s) 499, 506
MalI GATC 5 cut(s) 135, 217, 267, 358, 458
MbiI CCGCTC 1 cut(s) 294
MboI GATC 5 cut(s) 133, 215, 265, 356, 456
MboII GAAGA 1 cut(s) 251
MflI RGATCY 2 cut(s) 265, 356
MhlI GDGCHC 3 cut(s) 321, 487, 638
MluCI AATT 4 cut(s) 76, 116, 278, 520
MmeI TCCRAC 3 cut(s) 42, 196, 578
MnlI CCTC 3 cut(s) 206, 328, 581
MseI TTAA 2 cut(s) 161, 519
MslI CAYNNNNRTG 1 cut(s) 631
MspR9I CCNGG 1 cut(s) 43
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 398, 540
NdeII GATC 5 cut(s) 133, 215, 265, 356, 456
NlaIII CATG 3 cut(s) 21, 201, 419
NspI RCATGY 1 cut(s) 419
PfeI GAWTC 3 cut(s) 140, 256, 386
PkrI GCNGC 2 cut(s) 545, 548
PshBI ATTAAT 1 cut(s) 519
Psp1406I AACGTT 2 cut(s) 499, 506
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PstI CTGCAG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 265, 356
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 631
SaqAI TTAA 2 cut(s) 161, 519
SatI GCNGC 2 cut(s) 544, 547
Sau3AI GATC 5 cut(s) 133, 215, 265, 356, 456
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 3 cut(s) 321, 487, 638
SfaNI GCATC 2 cut(s) 112, 635
SfcI CTRYAG 1 cut(s) 390
SmiMI CAYNNNNRTG 1 cut(s) 631
SmlI CTYRAG 3 cut(s) 332, 462, 468
SmoI CTYRAG 3 cut(s) 332, 462, 468
Sse9I AATT 4 cut(s) 76, 116, 278, 520
SsiI CCGC 2 cut(s) 292, 547
SspI AATATT 1 cut(s) 538
SspMI CTAG 2 cut(s) 243, 360
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 2 cut(s) 454, 559
TaiI ACGT 2 cut(s) 502, 509
TaqII GACCGA 1 cut(s) 168
TasI AATT 4 cut(s) 76, 116, 278, 520
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 3 cut(s) 140, 256, 386
Tru1I TTAA 2 cut(s) 161, 519
Tru9I TTAA 2 cut(s) 161, 519
TscAI CASTG 1 cut(s) 636
TseI GCWGC 1 cut(s) 543
TspDTI ATGAA 3 cut(s) 81, 89, 315
TspRI CASTG 1 cut(s) 636
VneI GTGCAC 2 cut(s) 483, 634
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 3 cut(s) 76, 116, 278
XbaI TCTAGA 1 cut(s) 359
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 366
XspI CTAG 2 cut(s) 243, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.