Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011955

Physical Location & Seq

Genomic Coordinates Forward (+)

303811 .. 308365

Gene Structure 4555 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1001700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 675 bp

ATGGAGGTGCCGAGCTCATGGGACGCTCTTCGCAAGCAGGCAAGGAAACTTGAAGCTCAGTTGGATGAGCAGATGAATTCTTATCGTAAGTTTGTCTCTGCAAAGGGTTCTGCAAAAGTTGGTACTGCTGAGAATGATCTTGAATCTGTGATAGATCGACTATTAAAGCAACTCCAACAAGTAAATTCACAAATGCAGGCATGGGTATCATCAGGAGGATCAGAAATGGTTTCTCATACCTTGACTAGACATCAAGAAATTTTCCAAGATCTCACTCAGGAGTTCTATCGTCTCCGCAACAGCCTTAGAGCTAAGCAAGAGCATGCTTCACTCCTTGAGGATTTTAGGGAGTTTGATCGAACAAGAGTAGACTTGGAAGATGGTGTTGGCTCTGCGGAAAACGCTCTCCTGAAAGAGCATGCTGCTGTTAGCCGAAGTACAGGACAGGTGGATACTGTGATCTCTCAAGCTCAAGCAACCCTTGGTTCACTTGTCCTTCAACGCTCCACTTTTGGTGGTATTAATTCGAAGCTCAGCAATATTGGCAGCCGCCTTCCAACGGTAAATCAAATGCTGTCTGCTATAAAGCGTAAAAAGTCTCTGGATACCATCATTCTTTCTCTCGTTGCGTCTGTATGCACATTTCTCATATTTATCTACTGGTTGTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005795 GO:0005797 GO:0005801 GO:0006810 GO:0006888 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031090 GO:0031201 GO:0031984 GO:0031985 GO:0032879 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048209 GO:0048284 GO:0050789 GO:0050794 GO:0051049 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060341 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0090174 GO:0098588 GO:0098791 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 map04130

Pfam Domains

Protein Families

V-SNARE_C

Protein Analysis

225

Amino Acids

25.09

Weight (kDa)

9.01

Isoelectric Point (pI)

38.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
V-SNARE_C	PF12352	135 - 199	2.6e-17	Snare region anchored in the vesicle membrane C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0011955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15880
fragaria_vesca	FvH4_6g28830
malus_domestica	MD14G1001700.v1.1
prunus_persica	Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1
pyrus_communis	pycom12g00170 pycom49g00130
rosa_chinensis	RchiOBHm_Chr2g0133301 RchiOBHm_Chr2g0133361
rosa_laevigata	RLG00000019325
rosa_multiflora	Rmu_sc0003874.1_g000012
rosa_roxburghii	Rroxscaffold_2G00111230
rosa_rugosa	Rorug02G0311200
rosa_samantha	Rh2AG363700 Rh2BG369500 Rh2CG346900 Rh2DG386400
rosa_wichuraiana	Rw2G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	7
AccI	GTMKAC	1 cut(s)	369
AciI	CCGC	3 cut(s)	295, 395, 550
AclWI	GGATC	1 cut(s)	226
AcsI	RAATTY	3 cut(s)	76, 184, 258
AfaI	GTAC	2 cut(s)	124, 439
AfiI	CCNNNNNNNGG	1 cut(s)	559
AgsI	TTSAA	3 cut(s)	53, 143, 500
AjuI	GAANNNNNNNTTGG	2 cut(s)	369, 401
AluBI	AGCT	5 cut(s)	15, 56, 311, 470, 532
AluI	AGCT	5 cut(s)	15, 56, 311, 470, 532
Alw21I	GWGCWC	1 cut(s)	17
Alw26I	GTCTC	3 cut(s)	100, 296, 603
AlwI	GGATC	1 cut(s)	226
ApeKI	GCWGC	2 cut(s)	422, 546
ApoI	RAATTY	3 cut(s)	76, 184, 258
AseI	ATTAAT	1 cut(s)	522
AsuII	TTCGAA	1 cut(s)	527
BanI	GGYRCC	1 cut(s)	7
BanII	GRGCYC	1 cut(s)	17
Bbv12I	GWGCWC	1 cut(s)	17
BbvI	GCAGC	2 cut(s)	409, 558
BccI	CCATC	2 cut(s)	374, 617
BciVI	GTATCC	2 cut(s)	445, 598
BcoDI	GTCTC	3 cut(s)	100, 296, 603
BfaI	CTAG	1 cut(s)	246
BfuI	GTATCC	2 cut(s)	445, 598
BglII	AGATCT	1 cut(s)	268
BisI	GCNGC	3 cut(s)	423, 547, 550
BlpI	GCTNAGC	2 cut(s)	312, 533
BlsI	GCNGC	3 cut(s)	424, 548, 551
BmiI	GGNNCC	1 cut(s)	9
Bpu1102I	GCTNAGC	2 cut(s)	312, 533
Bpu14I	TTCGAA	1 cut(s)	527
BpuEI	CTTGAG	3 cut(s)	356, 450, 456
BsaJI	CCNNGG	1 cut(s)	481
Bsc4I	CCNNNNNNNGG	1 cut(s)	559
Bse1I	ACTGG	1 cut(s)	665
BseDI	CCNNGG	1 cut(s)	481
BseGI	GGATG	1 cut(s)	70
BseLI	CCNNNNNNNGG	1 cut(s)	559
BseMII	CTCAG	4 cut(s)	71, 120, 290, 547
BseNI	ACTGG	1 cut(s)	665
BseXI	GCAGC	2 cut(s)	409, 558
BshNI	GGYRCC	1 cut(s)	7
BsiHKAI	GWGCWC	1 cut(s)	17
BslFI	GGGAC	1 cut(s)	35
BslI	CCNNNNNNNGG	1 cut(s)	559
BsmAI	GTCTC	3 cut(s)	100, 296, 603
BsmBI	CGTCTC	1 cut(s)	296
BsmFI	GGGAC	1 cut(s)	35
Bsp119I	TTCGAA	1 cut(s)	527
Bsp1286I	GDGCHC	1 cut(s)	17
Bsp143I	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
Bsp1720I	GCTNAGC	2 cut(s)	312, 533
BspACI	CCGC	3 cut(s)	295, 395, 550
BspCNI	CTCAG	4 cut(s)	70, 121, 289, 546
BspLI	GGNNCC	1 cut(s)	9
BspPI	GGATC	1 cut(s)	226
BspQI	GCTCTTC	1 cut(s)	33
BspT104I	TTCGAA	1 cut(s)	527
BspT107I	GGYRCC	1 cut(s)	7
BsrI	ACTGG	1 cut(s)	665
BssECI	CCNNGG	1 cut(s)	481
BssMI	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
BssT1I	CCWWGG	1 cut(s)	481
Bst4CI	ACNGT	2 cut(s)	457, 562
Bst6I	CTCTTC	1 cut(s)	33
BstBI	TTCGAA	1 cut(s)	527
BstC8I	GCNNGC	5 cut(s)	35, 39, 198, 324, 420
BstDEI	CTNAG	6 cut(s)	57, 129, 276, 305, 312, 533
BstF5I	GGATG	1 cut(s)	70
BstKTI	GATC	6 cut(s)	139, 157, 221, 271, 358, 462
BstMAI	GTCTC	3 cut(s)	100, 296, 603
BstMBI	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
BstMWI	GCNNNNNNNGC	2 cut(s)	401, 543
BstNSI	RCATGY	2 cut(s)	326, 422
BstV1I	GCAGC	2 cut(s)	409, 558
BstX2I	RGATCY	1 cut(s)	268
BstYI	RGATCY	1 cut(s)	268
BsuI	GTATCC	2 cut(s)	445, 598
BtsCI	GGATG	1 cut(s)	70
Cac8I	GCNNGC	5 cut(s)	35, 39, 198, 324, 420
CseI	GACGC	2 cut(s)	32, 618
Csp6I	GTAC	2 cut(s)	123, 438
CviAII	CATG	4 cut(s)	18, 201, 323, 419
CviJI	RGCY	9 cut(s)	15, 56, 303, 311, 390, 432, 470, 532, 549
CviKI_1	RGCY	9 cut(s)	15, 56, 303, 311, 390, 432, 470, 532, 549
CviQI	GTAC	2 cut(s)	123, 438
DdeI	CTNAG	6 cut(s)	57, 129, 276, 305, 312, 533
DpnI	GATC	6 cut(s)	138, 156, 220, 270, 357, 461
DpnII	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
Eam1104I	CTCTTC	1 cut(s)	33
EarI	CTCTTC	1 cut(s)	33
Ecl136II	GAGCTC	1 cut(s)	15
Eco130I	CCWWGG	1 cut(s)	481
Eco24I	GRGCYC	1 cut(s)	17
Eco53kI	GAGCTC	1 cut(s)	15
EcoICRI	GAGCTC	1 cut(s)	15
EcoRI	GAATTC	1 cut(s)	76
EcoT14I	CCWWGG	1 cut(s)	481
EcoT38I	GRGCYC	1 cut(s)	17
ErhI	CCWWGG	1 cut(s)	481
Esp3I	CGTCTC	1 cut(s)	296
FaeI	CATG	4 cut(s)	21, 204, 326, 422
FaiI	YATR	8 cut(s)	19, 202, 237, 324, 420, 584, 637, 650
FalI	AAGNNNNNCTT	2 cut(s)	465, 497
FaqI	GGGAC	1 cut(s)	35
FatI	CATG	4 cut(s)	17, 200, 322, 418
FblI	GTMKAC	1 cut(s)	369
Fnu4HI	GCNGC	3 cut(s)	423, 547, 550
FokI	GGATG	1 cut(s)	77
FriOI	GRGCYC	1 cut(s)	17
Fsp4HI	GCNGC	3 cut(s)	423, 547, 550
FspBI	CTAG	1 cut(s)	246
GluI	GCNGC	3 cut(s)	423, 547, 550
HgaI	GACGC	2 cut(s)	32, 618
Hin1II	CATG	4 cut(s)	21, 204, 326, 422
HinfI	GANTC	1 cut(s)	143
Hpy166II	GTNNAC	2 cut(s)	370, 488
Hpy188I	TCNGA	1 cut(s)	223
Hpy188III	TCNNGA	6 cut(s)	140, 213, 254, 278, 409, 602
Hpy8I	GTNNAC	2 cut(s)	370, 488
HpyAV	CCTTC	2 cut(s)	506, 563
HpyCH4III	ACNGT	2 cut(s)	457, 562
HpyCH4V	TGCA	4 cut(s)	101, 113, 196, 639
HpyF10VI	GCNNNNNNNGC	2 cut(s)	401, 543
HpyF3I	CTNAG	6 cut(s)	57, 129, 276, 305, 312, 533
Hsp92II	CATG	4 cut(s)	21, 204, 326, 422
Kzo9I	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
LguI	GCTCTTC	1 cut(s)	33
LmnI	GCTCC	1 cut(s)	509
LpnPI	CCDG	9 cut(s)	23, 182, 198, 263, 422, 426, 431, 587, 646
Lsp1109I	GCAGC	2 cut(s)	409, 558
MaeI	CTAG	1 cut(s)	246
MalI	GATC	6 cut(s)	138, 156, 220, 270, 357, 461
MboI	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
MboII	GAAGA	2 cut(s)	20, 389
MflI	RGATCY	1 cut(s)	268
MhlI	GDGCHC	1 cut(s)	17
MluCI	AATT	4 cut(s)	76, 184, 258, 523
MmeI	TCCRAC	3 cut(s)	42, 199, 581
MnlI	CCTC	2 cut(s)	209, 331
MseI	TTAA	2 cut(s)	164, 522
MwoI	GCNNNNNNNGC	2 cut(s)	401, 543
NdeII	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
NlaIII	CATG	4 cut(s)	21, 204, 326, 422
NlaIV	GGNNCC	1 cut(s)	9
NmeAIII	GCCGAG	1 cut(s)	36
NspI	RCATGY	2 cut(s)	326, 422
NspV	TTCGAA	1 cut(s)	527
PaeI	GCATGC	2 cut(s)	326, 422
PciSI	GCTCTTC	1 cut(s)	33
PfeI	GAWTC	1 cut(s)	143
PkrI	GCNGC	3 cut(s)	424, 548, 551
PshBI	ATTAAT	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	17
PspN4I	GGNNCC	1 cut(s)	9
PsuI	RGATCY	1 cut(s)	268
RsaI	GTAC	2 cut(s)	124, 439
RsaNI	GTAC	2 cut(s)	123, 438
SacI	GAGCTC	1 cut(s)	17
SapI	GCTCTTC	1 cut(s)	33
SaqAI	TTAA	2 cut(s)	164, 522
SatI	GCNGC	3 cut(s)	423, 547, 550
Sau3AI	GATC	6 cut(s)	136, 154, 218, 268, 355, 459
SduI	GDGCHC	1 cut(s)	17
SetI	ASST	8 cut(s)	9, 17, 58, 242, 313, 450, 472, 534
SfuI	TTCGAA	1 cut(s)	527
SmlI	CTYRAG	3 cut(s)	335, 465, 471
SmoI	CTYRAG	3 cut(s)	335, 465, 471
SphI	GCATGC	2 cut(s)	326, 422
Sse9I	AATT	4 cut(s)	76, 184, 258, 523
SsiI	CCGC	3 cut(s)	295, 395, 550
SspI	AATATT	1 cut(s)	541
SspMI	CTAG	1 cut(s)	246
SstI	GAGCTC	1 cut(s)	17
StyI	CCWWGG	1 cut(s)	481
TaaI	ACNGT	2 cut(s)	457, 562
TaqI	TCGA	3 cut(s)	157, 358, 527
TasI	AATT	4 cut(s)	76, 184, 258, 523
TatI	WGTACW	1 cut(s)	437
TauI	GCSGC	1 cut(s)	552
TfiI	GAWTC	1 cut(s)	143
Tru1I	TTAA	2 cut(s)	164, 522
Tru9I	TTAA	2 cut(s)	164, 522
TseI	GCWGC	2 cut(s)	422, 546
TspDTI	ATGAA	1 cut(s)	89
VspI	ATTAAT	1 cut(s)	522
XapI	RAATTY	3 cut(s)	76, 184, 258
XceI	RCATGY	2 cut(s)	326, 422
XmiI	GTMKAC	1 cut(s)	369
XspI	CTAG	1 cut(s)	246

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.