RchiOBHm_Chr2g0133301

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011955
Physical Location & Seq
Forward (+)
50026318 .. 50029907
3590 bp
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UTR
Exon/CDS
Intron
PRQ50449

Sequence Viewer

Length: 600 bp
ATGGATGTTCCAAGCTCATGGGACGCGCTTCGCAAACAGGCTAGGAAACTTGAAGCTCAGTTGGATGAACAGATGAATTCTTATCGTAAGTTTGTCTCTGCAAAGGGTTCTGTCAAATTTGATGCTGCTGATAATGATCTTGAATCTGGGATAGATCGACTATTAAAGCAACTCCAACAAGTAAATTTACAGATGCAAGCATGGGAATTTTATCGTCTCCGCTCCAGCCTTAGAGCTAAGCAGGAGCACGCTTCACTACTTGAGGATTTTAGGGAGTTTGATCGAACAAGAGTTGACTTGGAAGCTGGTGGAGATTCTGCAGGAAACGCTCTCCTGAAAGAACATGCTTCTGTTAGCCGAAGTACAGGACAGATGGATACTGTGATCTCTCAAGCTCAAGCAACTCTGGGTGCACTTGTCCTTCAACGTTCAACATTTGGCGGTATTAACTCAAAGCTCAGCAACATTGGCAGCCGCCTCCCAACGGTAAGTCACATCCTTTCTGCAATAAAGCGTAAAAAGTCTATGGATACCATCATTCTTTCACTTGTCGCATCAGTGTGCACATTTCTCATATTTATCTACTGGTTGACAAAGTGA

Protein Analysis

199

Amino Acids

22.28

Weight (kDa)

9.41

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 110 - 174 2.3e-18 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15880
fragaria_vesca FvH4_6g28830
malus_domestica MD14G1001700.v1.1
prunus_persica Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1 Prupe.3G096500_v2.0.a1
pyrus_communis pycom12g00170 pycom49g00130
rosa_chinensis RchiOBHm_Chr2g0133301 RchiOBHm_Chr2g0133361
rosa_laevigata RLG00000019325
rosa_multiflora Rmu_sc0003874.1_g000012
rosa_roxburghii Rroxscaffold_2G00111230
rosa_rugosa Rorug02G0311200
rosa_samantha Rh2AG363700 Rh2BG369500 Rh2CG346900 Rh2DG386400
rosa_wichuraiana Rw2G029600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 222
AccII CGCG 1 cut(s) 26
AciI CCGC 3 cut(s) 220, 441, 475
AclI AACGTT 1 cut(s) 427
AcsI RAATTY 4 cut(s) 76, 116, 184, 206
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 4 cut(s) 53, 143, 425, 432
AluBI AGCT 6 cut(s) 15, 56, 236, 305, 395, 457
AluI AGCT 6 cut(s) 15, 56, 236, 305, 395, 457
Alw21I GWGCWC 3 cut(s) 249, 415, 566
Alw26I GTCTC 2 cut(s) 100, 221
Alw44I GTGCAC 2 cut(s) 411, 562
ApaLI GTGCAC 2 cut(s) 411, 562
ApeKI GCWGC 2 cut(s) 125, 471
ApoI RAATTY 4 cut(s) 76, 116, 184, 206
AspLEI GCGC 1 cut(s) 28
BaeGI GKGCMC 2 cut(s) 415, 566
Bbv12I GWGCWC 3 cut(s) 249, 415, 566
BbvI GCAGC 2 cut(s) 112, 483
BccI CCATC 2 cut(s) 367, 542
BciVI GTATCC 2 cut(s) 370, 523
BcoDI GTCTC 2 cut(s) 100, 221
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 318
BfuI GTATCC 2 cut(s) 370, 523
BisI GCNGC 3 cut(s) 126, 472, 475
BlpI GCTNAGC 2 cut(s) 237, 458
BlsI GCNGC 3 cut(s) 127, 473, 476
BmsI GCATC 3 cut(s) 112, 183, 563
BpmI CTGGAG 1 cut(s) 208
Bpu1102I GCTNAGC 2 cut(s) 237, 458
BpuEI CTTGAG 3 cut(s) 281, 375, 381
BsaBI GATNNNNATC 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 590
Bse8I GATNNNNATC 1 cut(s) 135
BseGI GGATG 3 cut(s) 10, 70, 495
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 71, 472
BseNI ACTGG 1 cut(s) 590
BseSI GKGCMC 2 cut(s) 415, 566
BseXI GCAGC 2 cut(s) 112, 483
Bsh1236I CGCG 1 cut(s) 26
BsiHKAI GWGCWC 3 cut(s) 249, 415, 566
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 2 cut(s) 100, 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 35
Bsp1286I GDGCHC 3 cut(s) 249, 415, 566
Bsp143I GATC 4 cut(s) 136, 154, 280, 384
Bsp1720I GCTNAGC 2 cut(s) 237, 458
BspACI CCGC 3 cut(s) 220, 441, 475
BspCNI CTCAG 2 cut(s) 70, 471
BspFNI CGCG 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 322
BsrBI CCGCTC 1 cut(s) 222
BsrI ACTGG 1 cut(s) 590
BssMI GATC 4 cut(s) 136, 154, 280, 384
Bst4CI ACNGT 2 cut(s) 382, 487
BstC8I GCNNGC 2 cut(s) 198, 249
BstDEI CTNAG 4 cut(s) 57, 230, 237, 458
BstF5I GGATG 3 cut(s) 10, 70, 495
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 4 cut(s) 139, 157, 283, 387
BstMAI GTCTC 2 cut(s) 100, 221
BstMBI GATC 4 cut(s) 136, 154, 280, 384
BstMWI GCNNNNNNNGC 2 cut(s) 326, 468
BstNSI RCATGY 1 cut(s) 347
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 2 cut(s) 415, 566
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 112, 483
BstXI CCANNNNNNTGG 1 cut(s) 18
BsuI GTATCC 2 cut(s) 370, 523
BtsCI GGATG 3 cut(s) 10, 70, 495
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 2 cut(s) 198, 249
CfoI GCGC 1 cut(s) 28
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 363
CviAII CATG 3 cut(s) 18, 201, 344
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 4 cut(s) 57, 230, 237, 458
DpnI GATC 4 cut(s) 138, 156, 282, 386
DpnII GATC 4 cut(s) 136, 154, 280, 384
EcoRI GAATTC 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 3 cut(s) 21, 204, 347
FaiI YATR 5 cut(s) 19, 202, 345, 527, 575
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 17, 200, 343
Fnu4HI GCNGC 3 cut(s) 126, 472, 475
FokI GGATG 3 cut(s) 17, 77, 482
Fsp4HI GCNGC 3 cut(s) 126, 472, 475
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 27
GluI GCNGC 3 cut(s) 126, 472, 475
GsuI CTGGAG 1 cut(s) 208
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 28
Hin1II CATG 3 cut(s) 21, 204, 347
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 2 cut(s) 295, 591
HindII GTYRAC 2 cut(s) 295, 591
HinfI GANTC 2 cut(s) 143, 314
Hpy166II GTNNAC 4 cut(s) 295, 413, 564, 591
Hpy188III TCNNGA 2 cut(s) 140, 334
Hpy8I GTNNAC 4 cut(s) 295, 413, 564, 591
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 2 cut(s) 382, 487
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 6 cut(s) 101, 196, 320, 413, 506, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 468
HpyF3I CTNAG 4 cut(s) 57, 230, 237, 458
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 3 cut(s) 21, 204, 347
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 4 cut(s) 136, 154, 280, 384
LmnI GCTCC 2 cut(s) 227, 244
Lsp1109I GCAGC 2 cut(s) 112, 483
LweI GCATC 3 cut(s) 112, 183, 563
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 491
MalI GATC 4 cut(s) 138, 156, 282, 386
MbiI CCGCTC 1 cut(s) 222
MboI GATC 4 cut(s) 136, 154, 280, 384
MhlI GDGCHC 3 cut(s) 249, 415, 566
MluCI AATT 4 cut(s) 76, 116, 184, 206
MmeI TCCRAC 2 cut(s) 42, 199
MnlI CCTC 2 cut(s) 256, 488
MseI TTAA 2 cut(s) 164, 447
MslI CAYNNNNRTG 1 cut(s) 559
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 326, 468
NdeII GATC 4 cut(s) 136, 154, 280, 384
NlaIII CATG 3 cut(s) 21, 204, 347
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 1 cut(s) 347
PfeI GAWTC 2 cut(s) 143, 314
PkrI GCNGC 3 cut(s) 127, 473, 476
Psp1406I AACGTT 1 cut(s) 427
PstI CTGCAG 1 cut(s) 322
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 559
SaqAI TTAA 2 cut(s) 164, 447
SatI GCNGC 3 cut(s) 126, 472, 475
Sau3AI GATC 4 cut(s) 136, 154, 280, 384
SduI GDGCHC 3 cut(s) 249, 415, 566
SetI ASST 7 cut(s) 17, 58, 238, 307, 397, 430, 459
SfaNI GCATC 3 cut(s) 112, 183, 563
SfcI CTRYAG 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 559
SmlI CTYRAG 3 cut(s) 260, 390, 396
SmoI CTYRAG 3 cut(s) 260, 390, 396
Sse9I AATT 4 cut(s) 76, 116, 184, 206
SsiI CCGC 3 cut(s) 220, 441, 475
SspMI CTAG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 382, 487
TaiI ACGT 1 cut(s) 430
TaqI TCGA 2 cut(s) 157, 283
TasI AATT 4 cut(s) 76, 116, 184, 206
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 2 cut(s) 143, 314
Tru1I TTAA 2 cut(s) 164, 447
Tru9I TTAA 2 cut(s) 164, 447
TscAI CASTG 1 cut(s) 564
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 2 cut(s) 125, 471
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 81, 89
TspRI CASTG 1 cut(s) 564
VneI GTGCAC 2 cut(s) 411, 562
XapI RAATTY 4 cut(s) 76, 116, 184, 206
XceI RCATGY 1 cut(s) 347
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.