FvH4_6g32030

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016370
Physical Location & Seq
Forward (+)
25127142 .. 25128659
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32030.t1

Sequence Viewer

Length: 777 bp
ATGGCAGCAGAACCAAATAATTCAGAAGCTGAAAGCACTACCACGAGCAACTCATCATCTCTCTGTTCAGTAGCCTCTTCACCATGTTTATCTGGGTTGTTGCAGACTGCTACTCATCCCAAGTCACCTTCCAACACTACCAAGAATCCCAAAACAAGTCAGAAAGGTGACTCAAGCTCACTTCAGCAGAAGACCTCGCTTAAAAGAGCAAGAGAGCCTACAGATAATAACAACAACAGCAAACACCCGGTTTACAGAGGAGTTCGAATGCGCAACTGGGGCAAATGGGTGTCGGAAATTCGCGAGCCCCGCAAGAAGTCACGTATCTGGCTAGGCACATTTTCCACACCGGAAATGGCAGCCCGTGCCCACGACGTGGCCGCGCTGAGTATTAAGGGCAACTCGGCCATTCTGAACTTTCCAGAACTGGCCGAGTTGCTGCCTCGTCCGGAGTCACTCCAACCTCCGGACATTCAAGCTGCCGCGGCTAAAGCAGCAGCCATGGTTCATCTGGATTCCACCACCACAACATCGTCGTCGTCCACATCCTCATCCTCTTCCTCATCAGAGGGTTTGGAATCGGGAGAGGATGAGCTAGGCGAGATTGTGGAGCTACCCAACATAGAAGACAGCTTCAACTCAGGCGAGTCAAGAAGCGAGTTCATATTGGTGAACGACACGGTGGACATAGGTTGGCTGTATGATCATCCGCCATTGATGGGGCTCGATCACTTCGAAGAAGATCTAGTGTCTTGGGATATTCAAATGATTACTTGA

Protein Analysis

259

Amino Acids

27.96

Weight (kDa)

5.03

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 83 - 133 4.1e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 376
AccII CGCG 3 cut(s) 303, 383, 485
AccIII TCCGGA 2 cut(s) 448, 466
AciI CCGC 5 cut(s) 310, 381, 483, 485, 710
AcoI YGGCCR 3 cut(s) 378, 405, 429
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 376
AfiI CCNNNNNNNGG 3 cut(s) 376, 466, 719
AgsI TTSAA 3 cut(s) 476, 637, 764
AjiI CACGTC 1 cut(s) 376
AluBI AGCT 6 cut(s) 29, 177, 479, 595, 613, 633
AluI AGCT 6 cut(s) 29, 177, 479, 595, 613, 633
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 2 cut(s) 448, 466
AoxI GGCC 3 cut(s) 378, 405, 429
ApeKI GCWGC 6 cut(s) 5, 359, 439, 479, 494, 497
ApoI RAATTY 1 cut(s) 297
AspLEI GCGC 2 cut(s) 273, 385
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 4 cut(s) 72, 117, 179, 682
AsuII TTCGAA 2 cut(s) 265, 735
BaeGI GKGCMC 1 cut(s) 370
BanII GRGCYC 2 cut(s) 309, 726
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 197, 633
BbvI GCAGC 6 cut(s) 17, 371, 426, 466, 506, 509
BccI CCATC 1 cut(s) 712
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 248
BfaI CTAG 3 cut(s) 332, 596, 746
BfmI CTRYAG 1 cut(s) 219
BglII AGATCT 1 cut(s) 742
BisI GCNGC 9 cut(s) 6, 360, 381, 440, 480, 483, 486, 495, 498
BlsI GCNGC 9 cut(s) 7, 361, 382, 441, 481, 484, 487, 496, 499
Bme1390I CCNGG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 248
BmrI ACTGGG 1 cut(s) 286
BmuI ACTGGG 1 cut(s) 286
BpiI GAAGAC 2 cut(s) 197, 633
Bpu14I TTCGAA 2 cut(s) 265, 735
BpuEI CTTGAG 1 cut(s) 157
BpuMI CCSGG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 483, 501
BsaWI WCCGGW 3 cut(s) 349, 448, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 466, 719
Bse1I ACTGG 2 cut(s) 281, 432
BseAI TCCGGA 2 cut(s) 448, 466
BseDI CCNNGG 2 cut(s) 483, 501
BseGI GGATG 5 cut(s) 115, 545, 551, 595, 706
BseLI CCNNNNNNNGG 3 cut(s) 376, 466, 719
BseMII CTCAG 2 cut(s) 377, 654
BseNI ACTGG 2 cut(s) 281, 432
BseRI GAGGAG 1 cut(s) 273
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 6 cut(s) 17, 371, 426, 466, 506, 509
Bsh1236I CGCG 3 cut(s) 303, 383, 485
BshFI GGCC 3 cut(s) 380, 407, 431
BsiSI CCGG 4 cut(s) 248, 350, 449, 467
BslI CCNNNNNNNGG 3 cut(s) 376, 466, 719
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 3 cut(s) 380, 407, 431
Bsp119I TTCGAA 2 cut(s) 265, 735
Bsp1286I GDGCHC 3 cut(s) 309, 370, 726
Bsp13I TCCGGA 2 cut(s) 448, 466
Bsp143I GATC 3 cut(s) 703, 727, 742
Bsp19I CCATGG 1 cut(s) 501
Bsp68I TCGCGA 1 cut(s) 303
BspACI CCGC 5 cut(s) 310, 381, 483, 485, 710
BspANI GGCC 3 cut(s) 380, 407, 431
BspCNI CTCAG 2 cut(s) 378, 653
BspEI TCCGGA 2 cut(s) 448, 466
BspFNI CGCG 3 cut(s) 303, 383, 485
BspT104I TTCGAA 2 cut(s) 265, 735
BsrI ACTGG 2 cut(s) 281, 432
BssECI CCNNGG 2 cut(s) 483, 501
BssMI GATC 3 cut(s) 703, 727, 742
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 2 cut(s) 82, 562
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 323
BstBI TTCGAA 2 cut(s) 265, 735
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 2 cut(s) 386, 640
BstDSI CCRYGG 2 cut(s) 483, 501
BstF5I GGATG 5 cut(s) 115, 545, 551, 595, 706
BstFNI CGCG 3 cut(s) 303, 383, 485
BstHHI GCGC 2 cut(s) 273, 385
BstKTI GATC 3 cut(s) 706, 730, 745
BstMBI GATC 3 cut(s) 703, 727, 742
BstMWI GCNNNNNNNGC 6 cut(s) 279, 309, 365, 485, 491, 494
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 370
BstUI CGCG 3 cut(s) 303, 383, 485
BstV1I GCAGC 6 cut(s) 17, 371, 426, 466, 506, 509
BstV2I GAAGAC 2 cut(s) 197, 633
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 3 cut(s) 380, 407, 431
BtgI CCRYGG 2 cut(s) 483, 501
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 5 cut(s) 115, 545, 551, 595, 706
BtuMI TCGCGA 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 2 cut(s) 273, 385
Cfr42I CCGCGG 1 cut(s) 486
CviAII CATG 2 cut(s) 84, 502
DdeI CTNAG 2 cut(s) 386, 640
DpnI GATC 3 cut(s) 705, 729, 744
DpnII GATC 3 cut(s) 703, 727, 742
DraIII CACNNNGTG 1 cut(s) 376
EaeI YGGCCR 3 cut(s) 378, 405, 429
Eam1104I CTCTTC 2 cut(s) 82, 562
EarI CTCTTC 2 cut(s) 82, 562
EciI GGCGGA 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 2 cut(s) 309, 726
Eco57I CTGAAG 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 2 cut(s) 309, 726
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 2 cut(s) 87, 505
FaiI YATR 6 cut(s) 85, 503, 623, 665, 689, 702
FatI CATG 2 cut(s) 83, 501
FauI CCCGC 1 cut(s) 317
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 9 cut(s) 6, 360, 381, 440, 480, 483, 486, 495, 498
FokI GGATG 5 cut(s) 102, 532, 538, 602, 693
FriOI GRGCYC 2 cut(s) 309, 726
Fsp4HI GCNGC 9 cut(s) 6, 360, 381, 440, 480, 483, 486, 495, 498
FspBI CTAG 3 cut(s) 332, 596, 746
FspI TGCGCA 1 cut(s) 272
GlaI GCGC 2 cut(s) 272, 384
GluI GCNGC 9 cut(s) 6, 360, 381, 440, 480, 483, 486, 495, 498
HaeIII GGCC 3 cut(s) 380, 407, 431
HapII CCGG 4 cut(s) 248, 350, 449, 467
HhaI GCGC 2 cut(s) 273, 385
Hin1II CATG 2 cut(s) 87, 505
Hin6I GCGC 2 cut(s) 271, 383
HinP1I GCGC 2 cut(s) 271, 383
HinfI GANTC 6 cut(s) 145, 170, 452, 515, 578, 647
HpaII CCGG 4 cut(s) 248, 350, 449, 467
HphI GGTGA 4 cut(s) 72, 117, 179, 682
Hpy166II GTNNAC 4 cut(s) 253, 543, 673, 685
Hpy188I TCNGA 5 cut(s) 25, 162, 295, 414, 568
Hpy188III TCNNGA 7 cut(s) 302, 422, 449, 467, 512, 582, 651
Hpy8I GTNNAC 4 cut(s) 253, 543, 673, 685
Hpy99I CGWCG 3 cut(s) 377, 538, 541
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 2 cut(s) 322, 375
HpyCH4V TGCA 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 6 cut(s) 279, 309, 365, 485, 491, 494
HpyF3I CTNAG 2 cut(s) 386, 640
HpySE526I ACGT 2 cut(s) 322, 375
Hsp92II CATG 2 cut(s) 87, 505
HspAI GCGC 2 cut(s) 271, 383
Kpn2I TCCGGA 2 cut(s) 448, 466
Ksp22I TGATCA 1 cut(s) 703
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 3 cut(s) 703, 727, 742
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 6 cut(s) 17, 371, 426, 466, 506, 509
MaeI CTAG 3 cut(s) 332, 596, 746
MaeII ACGT 2 cut(s) 322, 375
MaeIII GTNAC 4 cut(s) 123, 167, 318, 453
MalI GATC 3 cut(s) 705, 729, 744
MboI GATC 3 cut(s) 703, 727, 742
MboII GAAGA 6 cut(s) 69, 202, 549, 638, 749, 752
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 3 cut(s) 309, 370, 726
MluCI AATT 2 cut(s) 19, 297
MlyI GAGTC 3 cut(s) 164, 461, 656
MmeI TCCRAC 3 cut(s) 156, 273, 484
MroI TCCGGA 2 cut(s) 448, 466
MseI TTAA 2 cut(s) 201, 393
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 4 cut(s) 248, 350, 449, 467
MspR9I CCNGG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 273
MvnI CGCG 3 cut(s) 303, 383, 485
MwoI GCNNNNNNNGC 6 cut(s) 279, 309, 365, 485, 491, 494
NciI CCSGG 1 cut(s) 248
NcoI CCATGG 1 cut(s) 501
NdeII GATC 3 cut(s) 703, 727, 742
NlaIII CATG 2 cut(s) 87, 505
NmeAIII GCCGAG 2 cut(s) 383, 457
NmuCI GTSAC 4 cut(s) 123, 167, 318, 453
NruI TCGCGA 1 cut(s) 303
NsbI TGCGCA 1 cut(s) 272
NspV TTCGAA 2 cut(s) 265, 735
PcsI WCGNNNNNNNCGW 1 cut(s) 732
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 3 cut(s) 145, 515, 578
PflMI CCANNNNNTGG 1 cut(s) 376
PkrI GCNGC 9 cut(s) 7, 361, 382, 441, 481, 484, 487, 496, 499
PleI GAGTC 3 cut(s) 164, 460, 655
PpsI GAGTC 3 cut(s) 164, 460, 655
Ppu21I YACGTR 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 742
RruI TCGCGA 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 668
SacII CCGCGG 1 cut(s) 486
SaqAI TTAA 2 cut(s) 201, 393
SatI GCNGC 9 cut(s) 6, 360, 381, 440, 480, 483, 486, 495, 498
Sau3AI GATC 3 cut(s) 703, 727, 742
SchI GAGTC 3 cut(s) 164, 461, 656
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 3 cut(s) 309, 370, 726
SfcI CTRYAG 1 cut(s) 219
Sfr303I CCGCGG 1 cut(s) 486
SfuI TTCGAA 2 cut(s) 265, 735
SgrBI CCGCGG 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
Sse9I AATT 2 cut(s) 19, 297
SsiI CCGC 5 cut(s) 310, 381, 483, 485, 710
SspMI CTAG 3 cut(s) 332, 596, 746
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 2 cut(s) 325, 378
TaqI TCGA 3 cut(s) 265, 726, 735
TasI AATT 2 cut(s) 19, 297
TauI GCSGC 3 cut(s) 383, 485, 488
TfiI GAWTC 3 cut(s) 145, 515, 578
Tru1I TTAA 2 cut(s) 201, 393
Tru9I TTAA 2 cut(s) 201, 393
TseFI GTSAC 4 cut(s) 123, 167, 318, 453
TseI GCWGC 6 cut(s) 5, 359, 439, 479, 494, 497
Tsp45I GTSAC 4 cut(s) 123, 167, 318, 453
TspDTI ATGAA 2 cut(s) 497, 652
Van91I CCANNNNNTGG 1 cut(s) 376
XapI RAATTY 1 cut(s) 297
XcmI CCANNNNNNNNNTGG 2 cut(s) 352, 508
XspI CTAG 3 cut(s) 332, 596, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.