MD17G1187300.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016370
Physical Location & Seq
Reverse (-)
22397623 .. 22398354
732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1187300.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCACCAGAACCAAACATTTCAGAAGCTGAAAGCACCAGCAACTCACCCTCATGCTCGTCTGGTTTGTTGCATACTCGCCCGAATTCAACCTCCAACACGCCCAACACCATAACCAAGAAGCCCAGCTCAGAGCAGCAGCAGCAAGTAATACTCAAAAGAAAGAGACATGATGATAGCAACGTCAACAGCAGCAAACACCCAGTTTACAGAGGAGTTCGTATGCGCAACTGGGGAAAATGGGTGTCTGAAATTCGCGAGCCGCGCAAGAAGTCAAGAATCTGGCTTGGCACATTCTCAACTCCTGAGATGGCAGCCCGTGCGCATGACGTAGCCGCCCTCAGCATTAAGGGCAGCTCCGCCATTCTGAACTTTCCAGAATTGACCGAGTTGTTGCCACGGCCAGCGTCCCTTAAACCTCAAGATGTCCAGGCTGCCGCGACCAAAGCAGCAGCCATGGTTCATTTAAATTCTACAACATCATCATCATCGTTATCATCATCGTCCTCCTCCTCTTCCTTGTTTTTGTCGCAGTCCGAGGGTTCGGATTCTGAGGAGCTAGGGGAGATTGTGGAGTTACCCAATATCGAGGGCAGTTTCGAGTCGCCCGAGTCGAGGAACGAGTTCATACTGGTTAATGACTCATTGGATGCGTGGGTGTATCCATCACTAGGTCTCGAGGGAGTTGAGTTAAACTTGTGGGGATTTTTCTCATCAGATATTAGCAGCTGA

Protein Analysis

244

Amino Acids

26.45

Weight (kDa)

5.61

Isoelectric Point (pI)

67.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 68 - 118 3.9e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 227, 324
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 3 cut(s) 258, 265, 440
AciI CCGC 4 cut(s) 263, 336, 360, 438
AcoI YGGCCR 1 cut(s) 401
AcsI RAATTY 3 cut(s) 85, 252, 469
AfiI CCNNNNNNNGG 2 cut(s) 615, 671
AgsI TTSAA 1 cut(s) 90
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 5 cut(s) 29, 129, 357, 559, 729
AluI AGCT 5 cut(s) 29, 129, 357, 559, 729
Alw26I GTCTC 2 cut(s) 160, 680
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 2 cut(s) 608, 677
AoxI GGCC 1 cut(s) 401
ApoI RAATTY 3 cut(s) 85, 252, 469
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 3 cut(s) 228, 267, 325
AsuHPI GGTGA 1 cut(s) 39
AvaI CYCGRG 2 cut(s) 608, 677
BanI GGYRCC 1 cut(s) 4
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 9 cut(s) 148, 151, 154, 204, 326, 366, 421, 461, 464
BccI CCATC 2 cut(s) 304, 673
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 431
BciVI GTATCC 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 160, 680
BfaI CTAG 2 cut(s) 560, 671
BfuI GTATCC 1 cut(s) 672
Bme1390I CCNGG 1 cut(s) 431
BmeT110I CYCGRG 2 cut(s) 608, 677
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 431
BmrI ACTGGG 2 cut(s) 197, 241
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 2 cut(s) 197, 241
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 3 cut(s) 398, 456, 537
Bsc4I CCNNNNNNNGG 2 cut(s) 615, 671
Bse1I ACTGG 3 cut(s) 203, 236, 636
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 398, 456, 537
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 615, 671
BseMII CTCAG 4 cut(s) 144, 297, 355, 543
BseNI ACTGG 3 cut(s) 203, 236, 636
BseRI GAGGAG 4 cut(s) 228, 499, 502, 569
BseXI GCAGC 9 cut(s) 148, 151, 154, 204, 326, 366, 421, 461, 464
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 3 cut(s) 258, 265, 440
BshFI GGCC 1 cut(s) 403
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 2 cut(s) 608, 677
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 2 cut(s) 615, 671
BsmAI GTCTC 2 cut(s) 160, 680
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 1 cut(s) 403
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 2 cut(s) 608, 677
Bsp19I CCATGG 1 cut(s) 456
Bsp68I TCGCGA 1 cut(s) 258
BspACI CCGC 4 cut(s) 263, 336, 360, 438
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 4 cut(s) 143, 298, 354, 544
BspFNI CGCG 3 cut(s) 258, 265, 440
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 680
BsrI ACTGG 3 cut(s) 203, 236, 636
BssECI CCNNGG 3 cut(s) 398, 456, 537
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 431
Bst6I CTCTTC 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 260, 405
BstDEI CTNAG 4 cut(s) 130, 306, 341, 552
BstDSI CCRYGG 2 cut(s) 398, 456
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 3 cut(s) 258, 265, 440
BstHHI GCGC 3 cut(s) 228, 267, 325
BstMAI GTCTC 2 cut(s) 160, 680
BstMWI GCNNNNNNNGC 5 cut(s) 142, 264, 320, 351, 446
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 429
BstUI CGCG 3 cut(s) 258, 265, 440
BstV1I GCAGC 9 cut(s) 148, 151, 154, 204, 326, 366, 421, 461, 464
BsuI GTATCC 1 cut(s) 672
BsuRI GGCC 1 cut(s) 403
BtgI CCRYGG 2 cut(s) 398, 456
BtsCI GGATG 1 cut(s) 655
BtuMI TCGCGA 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 260, 405
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 3 cut(s) 228, 267, 325
CseI GACGC 1 cut(s) 396
CviAII CATG 4 cut(s) 54, 170, 326, 457
DdeI CTNAG 4 cut(s) 130, 306, 341, 552
DraI TTTAAA 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 680
Eco88I CYCGRG 2 cut(s) 608, 677
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 4 cut(s) 57, 173, 329, 460
FaiI YATR 8 cut(s) 55, 75, 113, 171, 224, 327, 458, 629
FaqI GGGAC 1 cut(s) 394
FatI CATG 4 cut(s) 53, 169, 325, 456
FokI GGATG 1 cut(s) 662
FspAI RTGCGCAY 1 cut(s) 324
FspBI CTAG 2 cut(s) 560, 671
FspI TGCGCA 2 cut(s) 227, 324
GlaI GCGC 3 cut(s) 227, 266, 324
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 1 cut(s) 403
HgaI GACGC 1 cut(s) 396
HhaI GCGC 3 cut(s) 228, 267, 325
Hin1II CATG 4 cut(s) 57, 173, 329, 460
Hin6I GCGC 3 cut(s) 226, 265, 323
HinP1I GCGC 3 cut(s) 226, 265, 323
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 279, 548, 602, 611, 641
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 2 cut(s) 187, 208
Hpy188I TCNGA 8 cut(s) 25, 133, 250, 369, 538, 547, 553, 718
Hpy188III TCNNGA 6 cut(s) 257, 276, 305, 377, 422, 677
Hpy8I GTNNAC 2 cut(s) 187, 208
HpyCH4IV ACGT 2 cut(s) 183, 330
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 264, 320, 351, 446
HpyF3I CTNAG 4 cut(s) 130, 306, 341, 552
HpySE526I ACGT 2 cut(s) 183, 330
Hsp92II CATG 4 cut(s) 57, 173, 329, 460
HspAI GCGC 3 cut(s) 226, 265, 323
LmnI GCTCC 2 cut(s) 362, 556
Lsp1109I GCAGC 9 cut(s) 148, 151, 154, 204, 326, 366, 421, 461, 464
LweI GCATC 1 cut(s) 640
MaeI CTAG 2 cut(s) 560, 671
MaeII ACGT 2 cut(s) 183, 330
MaeIII GTNAC 1 cut(s) 576
MboII GAAGA 1 cut(s) 507
MluCI AATT 4 cut(s) 85, 252, 380, 469
MlyI GAGTC 3 cut(s) 611, 620, 635
MmeI TCCRAC 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 5 cut(s) 348, 414, 467, 636, 692
MslI CAYNNNNRTG 1 cut(s) 52
MspA1I CMGCKG 1 cut(s) 729
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 3 cut(s) 258, 265, 440
MwoI GCNNNNNNNGC 5 cut(s) 142, 264, 320, 351, 446
NcoI CCATGG 1 cut(s) 456
NlaIII CATG 4 cut(s) 57, 173, 329, 460
NlaIV GGNNCC 1 cut(s) 6
NruI TCGCGA 1 cut(s) 258
NsbI TGCGCA 2 cut(s) 227, 324
PaeR7I CTCGAG 1 cut(s) 677
PcsI WCGNNNNNNNCGW 2 cut(s) 606, 611
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 2 cut(s) 279, 548
PleI GAGTC 3 cut(s) 610, 619, 635
PpsI GAGTC 3 cut(s) 610, 619, 635
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 729
RruI TCGCGA 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 52
SaqAI TTAA 5 cut(s) 348, 414, 467, 636, 692
SchI GAGTC 3 cut(s) 611, 620, 635
ScrFI CCNGG 1 cut(s) 431
SfaNI GCATC 1 cut(s) 640
Sfr274I CTCGAG 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 677
SmiI ATTTAAAT 1 cut(s) 468
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 2 cut(s) 420, 677
SmoI CTYRAG 2 cut(s) 420, 677
Sse9I AATT 4 cut(s) 85, 252, 380, 469
SsiI CCGC 4 cut(s) 263, 336, 360, 438
SspMI CTAG 2 cut(s) 560, 671
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 456
SwaI ATTTAAAT 1 cut(s) 468
TaiI ACGT 2 cut(s) 186, 333
TaqI TCGA 4 cut(s) 588, 600, 614, 678
TaqII GACCGA 1 cut(s) 401
TasI AATT 4 cut(s) 85, 252, 380, 469
TauI GCSGC 3 cut(s) 265, 338, 440
TfiI GAWTC 2 cut(s) 279, 548
Tru1I TTAA 5 cut(s) 348, 414, 467, 636, 692
Tru9I TTAA 5 cut(s) 348, 414, 467, 636, 692
TspDTI ATGAA 2 cut(s) 452, 616
XapI RAATTY 3 cut(s) 85, 252, 469
XhoI CTCGAG 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 2 cut(s) 560, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.