RLG00000019824

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016370
Physical Location & Seq
Forward (+)
57152439 .. 57153233
795 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019824

Sequence Viewer

Length: 795 bp
ATGGCTGCAGAACCAAACAATTCAGAAGCTGAAAGCACCACGAGCAACTCATCTCTATGTTCAGTAGCCTCTTCTCCGTGCTTATCTGGGTTGTTGCAGAGTACTACTCATCCCAACTCACCCTCCAACACTACCAAGAATCCCAAAACAAGTCAGAAAGGTAACTCAAACTCAGAGCAGCAGCAGAAGTCGATTAAAAGAGGAAGAGAATCTAGAGATGATAATAACGGCAGCAAGCACCCGGTTTACAGAGGAGTACGAATGCGCAATTGGGGCAAATGGGTGTCGGAAATTCGCGAGCCTCGCAAGAAGTCACGTATCTGGCTAGGCACATTTTCGACAGCGGAAATGGCAGCCCGTGCCCACGACGTGGCCGCCCTGAGCATTAAGGGCAACTCGGCCATTCTAAACTTCCCAGAACTGGCCGAGTTGCTGCCCCGTCCGGAGTCACTCAAACCTCCGGACATTCAAGCTGCGGCGGCCAAAGCGGCAGCTATGGTTCATCTGAGTTCTACCACCACAACATCATCGTCGTCCACATCCTCATCCTCTTCCTCGTCCTCGTCAGAGGGTTTGGAATCCGAAGAGGACCTAGGCGAGATTGTGGAGTTACCCAACATAGAAGACAGCTTCAACTCAGGCGAGCCGGGAAGCGAGTTCATACTGGTGAACGACTCGGTGGACATGGCGTGGCTGTATGATCATCCGCCATTGTTGGGGCTCGATCACAACTTAATTCCAAGCATTACCTTTGAAGAAGATCTAGTGTCTTGGGATATTCAAATGATTACTTAA

Protein Analysis

265

Amino Acids

28.53

Weight (kDa)

5.04

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 81 - 131 5.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 266
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 1 cut(s) 297
AccIII TCCGGA 2 cut(s) 442, 460
AciI CCGC 6 cut(s) 344, 375, 476, 479, 488, 707
AcoI YGGCCR 4 cut(s) 372, 399, 423, 480
AcsI RAATTY 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 103, 258
AfiI CCNNNNNNNGG 3 cut(s) 370, 421, 716
AgsI TTSAA 4 cut(s) 470, 634, 755, 782
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 4 cut(s) 29, 473, 494, 630
AluI AGCT 4 cut(s) 29, 473, 494, 630
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 2 cut(s) 442, 460
AoxI GGCC 4 cut(s) 372, 399, 423, 480
ApeKI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
ApoI RAATTY 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 592
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 589
AsuC2I CCSGG 2 cut(s) 242, 648
AsuHPI GGTGA 2 cut(s) 111, 679
AvaII GGWCC 1 cut(s) 589
AvrII CCTAGG 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 723
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 7 cut(s) 190, 193, 243, 365, 420, 460, 503
BceAI ACGGC 1 cut(s) 244
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 2 cut(s) 242, 648
BfaI CTAG 4 cut(s) 213, 326, 593, 764
BfmI CTRYAG 1 cut(s) 6
BglI GCCNNNNNGGC 1 cut(s) 488
BglII AGATCT 1 cut(s) 760
BlnI CCTAGG 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 2 cut(s) 242, 648
Bme18I GGWCC 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 589
BmrFI CCNGG 2 cut(s) 242, 648
BpiI GAAGAC 1 cut(s) 630
BplI GAGNNNNNCTC 2 cut(s) 91, 123
Bpu10I CCTNAGC 1 cut(s) 380
BpuMI CCSGG 2 cut(s) 242, 648
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 592
BsaWI WCCGGW 2 cut(s) 442, 460
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 421, 716
Bse1I ACTGG 2 cut(s) 426, 669
BseAI TCCGGA 2 cut(s) 442, 460
BseDI CCNNGG 1 cut(s) 592
BseGI GGATG 4 cut(s) 109, 539, 545, 703
BseLI CCNNNNNNNGG 3 cut(s) 370, 421, 716
BseMII CTCAG 4 cut(s) 186, 371, 497, 651
BseNI ACTGG 2 cut(s) 426, 669
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 7 cut(s) 190, 193, 243, 365, 420, 460, 503
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 4 cut(s) 374, 401, 425, 482
BsiSI CCGG 4 cut(s) 242, 443, 461, 647
BslI CCNNNNNNNGG 3 cut(s) 370, 421, 716
BsmI GAATGC 1 cut(s) 267
BsnI GGCC 4 cut(s) 374, 401, 425, 482
Bsp1286I GDGCHC 2 cut(s) 364, 723
Bsp13I TCCGGA 2 cut(s) 442, 460
Bsp143I GATC 3 cut(s) 700, 724, 760
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 6 cut(s) 344, 375, 476, 479, 488, 707
BspANI GGCC 4 cut(s) 374, 401, 425, 482
BspCNI CTCAG 4 cut(s) 185, 372, 498, 650
BspEI TCCGGA 2 cut(s) 442, 460
BspFNI CGCG 1 cut(s) 297
BspMAI CTGCAG 1 cut(s) 10
BsrI ACTGG 2 cut(s) 426, 669
BssECI CCNNGG 1 cut(s) 592
BssMI GATC 3 cut(s) 700, 724, 760
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 40
Bst6I CTCTTC 4 cut(s) 76, 199, 556, 579
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 236, 299, 644
BstDEI CTNAG 4 cut(s) 172, 380, 506, 637
BstF5I GGATG 4 cut(s) 109, 539, 545, 703
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 3 cut(s) 703, 727, 763
BstMBI GATC 3 cut(s) 700, 724, 760
BstMWI GCNNNNNNNGC 9 cut(s) 42, 273, 303, 350, 359, 390, 479, 485, 488
BstSCI CCNGG 2 cut(s) 240, 646
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 364
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 7 cut(s) 190, 193, 243, 365, 420, 460, 503
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
BsuRI GGCC 4 cut(s) 374, 401, 425, 482
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 4 cut(s) 109, 539, 545, 703
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 236, 299, 644
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 589
Csp6I GTAC 2 cut(s) 102, 257
CviAII CATG 1 cut(s) 685
CviQI GTAC 2 cut(s) 102, 257
DdeI CTNAG 4 cut(s) 172, 380, 506, 637
DpnI GATC 3 cut(s) 702, 726, 762
DpnII GATC 3 cut(s) 700, 724, 760
DraIII CACNNNGTG 1 cut(s) 370
EaeI YGGCCR 4 cut(s) 372, 399, 423, 480
Eam1104I CTCTTC 4 cut(s) 76, 199, 556, 579
EarI CTCTTC 4 cut(s) 76, 199, 556, 579
EciI GGCGGA 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 1 cut(s) 589
EcoO109I RGGNCCY 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 592
FaeI CATG 1 cut(s) 688
FaiI YATR 6 cut(s) 58, 497, 620, 662, 686, 699
FatI CATG 1 cut(s) 684
FbaI TGATCA 1 cut(s) 700
FokI GGATG 4 cut(s) 96, 526, 532, 690
FriOI GRGCYC 1 cut(s) 723
FspBI CTAG 4 cut(s) 213, 326, 593, 764
FspI TGCGCA 1 cut(s) 266
GlaI GCGC 1 cut(s) 266
HaeIII GGCC 4 cut(s) 374, 401, 425, 482
HapII CCGG 4 cut(s) 242, 443, 461, 647
HhaI GCGC 1 cut(s) 267
Hin1II CATG 1 cut(s) 688
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 5 cut(s) 139, 209, 446, 578, 674
HpaII CCGG 4 cut(s) 242, 443, 461, 647
HphI GGTGA 2 cut(s) 111, 679
Hpy166II GTNNAC 4 cut(s) 247, 537, 670, 682
Hpy188I TCNGA 7 cut(s) 25, 156, 175, 289, 507, 568, 583
Hpy188III TCNNGA 4 cut(s) 213, 296, 443, 461
Hpy8I GTNNAC 4 cut(s) 247, 537, 670, 682
Hpy99I CGWCG 2 cut(s) 371, 535
HpyCH4IV ACGT 2 cut(s) 316, 369
HpyCH4V TGCA 2 cut(s) 8, 97
HpyF10VI GCNNNNNNNGC 9 cut(s) 42, 273, 303, 350, 359, 390, 479, 485, 488
HpyF3I CTNAG 4 cut(s) 172, 380, 506, 637
HpySE526I ACGT 2 cut(s) 316, 369
Hsp92II CATG 1 cut(s) 688
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 2 cut(s) 442, 460
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 3 cut(s) 700, 724, 760
Lsp1109I GCAGC 7 cut(s) 190, 193, 243, 365, 420, 460, 503
MaeI CTAG 4 cut(s) 213, 326, 593, 764
MaeII ACGT 2 cut(s) 316, 369
MaeIII GTNAC 4 cut(s) 161, 312, 447, 609
MalI GATC 3 cut(s) 702, 726, 762
MboI GATC 3 cut(s) 700, 724, 760
MboII GAAGA 7 cut(s) 63, 216, 543, 596, 635, 767, 770
MfeI CAATTG 1 cut(s) 268
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 2 cut(s) 364, 723
MluCI AATT 4 cut(s) 19, 268, 291, 735
MlyI GAGTC 2 cut(s) 455, 668
MmeI TCCRAC 2 cut(s) 150, 267
MroI TCCGGA 2 cut(s) 442, 460
MseI TTAA 4 cut(s) 195, 387, 734, 793
MslI CAYNNNNRTG 2 cut(s) 55, 665
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 4 cut(s) 242, 443, 461, 647
MspR9I CCNGG 2 cut(s) 242, 648
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 267
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 9 cut(s) 42, 273, 303, 350, 359, 390, 479, 485, 488
NciI CCSGG 2 cut(s) 242, 648
NdeII GATC 3 cut(s) 700, 724, 760
NlaIII CATG 1 cut(s) 688
NmeAIII GCCGAG 2 cut(s) 377, 451
NmuCI GTSAC 2 cut(s) 312, 447
NruI TCGCGA 1 cut(s) 297
NsbI TGCGCA 1 cut(s) 266
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 3 cut(s) 139, 209, 578
PflMI CCANNNNNTGG 1 cut(s) 370
PleI GAGTC 2 cut(s) 454, 668
PpsI GAGTC 2 cut(s) 454, 668
Ppu21I YACGTR 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 589
PspPI GGNCC 1 cut(s) 589
PspPPI RGGWCCY 1 cut(s) 589
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 760
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 2 cut(s) 103, 258
RsaNI GTAC 2 cut(s) 102, 257
RseI CAYNNNNRTG 2 cut(s) 55, 665
SaqAI TTAA 4 cut(s) 195, 387, 734, 793
Sau3AI GATC 3 cut(s) 700, 724, 760
Sau96I GGNCC 1 cut(s) 589
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 2 cut(s) 455, 668
ScrFI CCNGG 2 cut(s) 242, 648
SduI GDGCHC 2 cut(s) 364, 723
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 55, 665
Sse9I AATT 4 cut(s) 19, 268, 291, 735
SsiI CCGC 6 cut(s) 344, 375, 476, 479, 488, 707
SspMI CTAG 4 cut(s) 213, 326, 593, 764
StyD4I CCNGG 2 cut(s) 240, 646
StyI CCWWGG 1 cut(s) 592
TaiI ACGT 2 cut(s) 319, 372
TaqI TCGA 3 cut(s) 191, 338, 723
TasI AATT 4 cut(s) 19, 268, 291, 735
TatI WGTACW 1 cut(s) 101
TauI GCSGC 4 cut(s) 377, 479, 482, 491
TfiI GAWTC 3 cut(s) 139, 209, 578
Tru1I TTAA 4 cut(s) 195, 387, 734, 793
Tru9I TTAA 4 cut(s) 195, 387, 734, 793
TseFI GTSAC 2 cut(s) 312, 447
TseI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
Tsp45I GTSAC 2 cut(s) 312, 447
TspDTI ATGAA 2 cut(s) 491, 649
TspGWI ACGGA 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 1 cut(s) 291
XbaI TCTAGA 1 cut(s) 212
XmaJI CCTAGG 1 cut(s) 592
XspI CTAG 4 cut(s) 213, 326, 593, 764
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.