Rw2G033480

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016370
Physical Location & Seq
Reverse (-)
54813024 .. 54814239
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033480.1

Sequence Viewer

Length: 795 bp
ATGGCAGCAGAACCAAACAATTCAGAAGCTGAAAGCACGACAAGCAACTCATCTCTATGTTCAGTAGCCTCTTCTCCGTGTTTATCTGGGTTGTTGCAGAGTACTACTCATTCCAACTCACCCTCCAACACTACCAAGAATCCCAAAACAAGTCAGAAAGGTGACTCAAACTCAGAGCAGCAGCAGAAGTCGCTTAAAAGAGGAAGAGAATCTAGAGATGATAATAACAGCAGCAAGCACCCGGTTTACAGAGGAGTACGAATGCGCAATTGGGGCAAATGGGTGTCCGAAATTCGCGAGCCTCGCAAGAAGTCACGTATCTGGCTAGGCACATTTTCGACAGCGGAAATGGCAGCCCGTGCCCACGACGTGGCCGCCCTGAGCATCAAGGGCAACTCGGCCATTCTAAACTTCCCAGAACTGGCCGGGTTGCTGCCCCGTCCGGAGTCACTCAAACCTCCGGACATTCAAGCTGCCGCGGCCAAAGCGGCAGCTATGGTTCATCTGAGTTCTACCATCACAACATCATCGTCGTCCACATCCTCATCCTCATCCTCTTCCTCGTCAGAGGGTTTGGAATCCGAAGAGGACCTAGGCGAGATTGTGGAGTTACCCAACATAGAAGACAGCTTCAACTCAGGCGAGCCGGGAAGCGAGTTCATACTGGTGAACGACTCGGTGGATATAGCGTGGCTGTATGATCATCCGCCATTATTTGGGCTCGATCACAACTTAATTCCAAGCATTACCTTTGAAGAAGATCTAGTGTCTTGGGATATTCAAATGATTACTTAA

Protein Analysis

264

Amino Acids

28.51

Weight (kDa)

5.03

Isoelectric Point (pI)

58.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 81 - 131 5.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 266
AccB7I CCANNNNNTGG 2 cut(s) 370, 716
AccII CGCG 2 cut(s) 297, 479
AccIII TCCGGA 2 cut(s) 442, 460
AciI CCGC 6 cut(s) 344, 375, 477, 479, 488, 707
AcoI YGGCCR 4 cut(s) 372, 399, 423, 480
AcsI RAATTY 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 103, 258
AfiI CCNNNNNNNGG 3 cut(s) 370, 421, 716
AgsI TTSAA 4 cut(s) 470, 634, 755, 782
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 4 cut(s) 29, 473, 494, 630
AluI AGCT 4 cut(s) 29, 473, 494, 630
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 2 cut(s) 442, 460
AoxI GGCC 4 cut(s) 372, 399, 423, 480
ApeKI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
ApoI RAATTY 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 592
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 589
AsuC2I CCSGG 3 cut(s) 242, 427, 648
AsuHPI GGTGA 3 cut(s) 111, 173, 679
AvaII GGWCC 1 cut(s) 589
AvrII CCTAGG 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
BccI CCATC 1 cut(s) 524
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 3 cut(s) 242, 427, 648
BfaI CTAG 4 cut(s) 213, 326, 593, 764
BglI GCCNNNNNGGC 1 cut(s) 488
BglII AGATCT 1 cut(s) 760
BlnI CCTAGG 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 3 cut(s) 242, 427, 648
Bme18I GGWCC 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 589
BmrFI CCNGG 3 cut(s) 242, 427, 648
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 630
BplI GAGNNNNNCTC 2 cut(s) 91, 123
Bpu10I CCTNAGC 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 242, 427, 648
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 477, 592
BsaWI WCCGGW 2 cut(s) 442, 460
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 421, 716
Bse1I ACTGG 2 cut(s) 426, 669
BseAI TCCGGA 2 cut(s) 442, 460
BseDI CCNNGG 2 cut(s) 477, 592
BseGI GGATG 4 cut(s) 539, 545, 551, 703
BseLI CCNNNNNNNGG 3 cut(s) 370, 421, 716
BseMII CTCAG 4 cut(s) 186, 371, 497, 651
BseNI ACTGG 2 cut(s) 426, 669
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
Bsh1236I CGCG 2 cut(s) 297, 479
BshFI GGCC 4 cut(s) 374, 401, 425, 482
BsiSI CCGG 5 cut(s) 242, 426, 443, 461, 647
BslI CCNNNNNNNGG 3 cut(s) 370, 421, 716
BsmI GAATGC 1 cut(s) 267
BsnI GGCC 4 cut(s) 374, 401, 425, 482
Bsp1286I GDGCHC 2 cut(s) 364, 723
Bsp13I TCCGGA 2 cut(s) 442, 460
Bsp143I GATC 3 cut(s) 700, 724, 760
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 6 cut(s) 344, 375, 477, 479, 488, 707
BspANI GGCC 4 cut(s) 374, 401, 425, 482
BspCNI CTCAG 4 cut(s) 185, 372, 498, 650
BspEI TCCGGA 2 cut(s) 442, 460
BspFNI CGCG 2 cut(s) 297, 479
BsrI ACTGG 2 cut(s) 426, 669
BssECI CCNNGG 2 cut(s) 477, 592
BssMI GATC 3 cut(s) 700, 724, 760
BssT1I CCWWGG 1 cut(s) 592
Bst6I CTCTTC 4 cut(s) 76, 199, 562, 579
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 236, 299, 644
BstDEI CTNAG 4 cut(s) 172, 380, 506, 637
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 539, 545, 551, 703
BstFNI CGCG 2 cut(s) 297, 479
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 3 cut(s) 703, 727, 763
BstMBI GATC 3 cut(s) 700, 724, 760
BstSCI CCNGG 3 cut(s) 240, 425, 646
BstSLI GKGCMC 1 cut(s) 364
BstUI CGCG 2 cut(s) 297, 479
BstV1I GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
BsuRI GGCC 4 cut(s) 374, 401, 425, 482
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 4 cut(s) 539, 545, 551, 703
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 236, 299, 644
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 589
Cfr42I CCGCGG 1 cut(s) 480
Csp6I GTAC 2 cut(s) 102, 257
CviQI GTAC 2 cut(s) 102, 257
DdeI CTNAG 4 cut(s) 172, 380, 506, 637
DpnI GATC 3 cut(s) 702, 726, 762
DpnII GATC 3 cut(s) 700, 724, 760
DraIII CACNNNGTG 1 cut(s) 370
EaeI YGGCCR 4 cut(s) 372, 399, 423, 480
Eam1104I CTCTTC 4 cut(s) 76, 199, 562, 579
EarI CTCTTC 4 cut(s) 76, 199, 562, 579
EciI GGCGGA 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 1 cut(s) 589
EcoO109I RGGNCCY 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 592
FaiI YATR 6 cut(s) 58, 497, 620, 662, 686, 699
FbaI TGATCA 1 cut(s) 700
FokI GGATG 4 cut(s) 526, 532, 538, 690
FriOI GRGCYC 1 cut(s) 723
FspBI CTAG 4 cut(s) 213, 326, 593, 764
FspI TGCGCA 1 cut(s) 266
GlaI GCGC 1 cut(s) 266
HaeIII GGCC 4 cut(s) 374, 401, 425, 482
HapII CCGG 5 cut(s) 242, 426, 443, 461, 647
HhaI GCGC 1 cut(s) 267
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 6 cut(s) 139, 164, 209, 446, 578, 674
HpaII CCGG 5 cut(s) 242, 426, 443, 461, 647
HphI GGTGA 3 cut(s) 111, 173, 679
Hpy166II GTNNAC 3 cut(s) 247, 537, 670
Hpy188I TCNGA 7 cut(s) 25, 156, 175, 289, 507, 568, 583
Hpy188III TCNNGA 4 cut(s) 213, 296, 443, 461
Hpy8I GTNNAC 3 cut(s) 247, 537, 670
Hpy99I CGWCG 2 cut(s) 371, 535
HpyCH4IV ACGT 2 cut(s) 316, 369
HpyCH4V TGCA 1 cut(s) 97
HpyF3I CTNAG 4 cut(s) 172, 380, 506, 637
HpySE526I ACGT 2 cut(s) 316, 369
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 2 cut(s) 442, 460
Ksp22I TGATCA 1 cut(s) 700
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 3 cut(s) 700, 724, 760
Lsp1109I GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
LweI GCATC 1 cut(s) 393
MaeI CTAG 4 cut(s) 213, 326, 593, 764
MaeII ACGT 2 cut(s) 316, 369
MaeIII GTNAC 4 cut(s) 161, 312, 447, 609
MalI GATC 3 cut(s) 702, 726, 762
MboI GATC 3 cut(s) 700, 724, 760
MboII GAAGA 7 cut(s) 63, 216, 549, 596, 635, 767, 770
MfeI CAATTG 1 cut(s) 268
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 2 cut(s) 364, 723
MluCI AATT 4 cut(s) 19, 268, 291, 735
MlyI GAGTC 3 cut(s) 158, 455, 668
MmeI TCCRAC 2 cut(s) 138, 150
MroI TCCGGA 2 cut(s) 442, 460
MseI TTAA 3 cut(s) 195, 734, 793
MslI CAYNNNNRTG 2 cut(s) 55, 665
MspA1I CMGCKG 2 cut(s) 344, 479
MspI CCGG 5 cut(s) 242, 426, 443, 461, 647
MspR9I CCNGG 3 cut(s) 242, 427, 648
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 267
MvnI CGCG 2 cut(s) 297, 479
NciI CCSGG 3 cut(s) 242, 427, 648
NdeII GATC 3 cut(s) 700, 724, 760
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 3 cut(s) 161, 312, 447
NruI TCGCGA 1 cut(s) 297
NsbI TGCGCA 1 cut(s) 266
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 3 cut(s) 139, 209, 578
PflMI CCANNNNNTGG 2 cut(s) 370, 716
PleI GAGTC 3 cut(s) 158, 454, 668
PpsI GAGTC 3 cut(s) 158, 454, 668
Ppu21I YACGTR 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 589
PspPI GGNCC 1 cut(s) 589
PspPPI RGGWCCY 1 cut(s) 589
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 760
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 2 cut(s) 103, 258
RsaNI GTAC 2 cut(s) 102, 257
RseI CAYNNNNRTG 2 cut(s) 55, 665
SacII CCGCGG 1 cut(s) 480
SaqAI TTAA 3 cut(s) 195, 734, 793
Sau3AI GATC 3 cut(s) 700, 724, 760
Sau96I GGNCC 1 cut(s) 589
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 3 cut(s) 158, 455, 668
ScrFI CCNGG 3 cut(s) 242, 427, 648
SduI GDGCHC 2 cut(s) 364, 723
SfaNI GCATC 1 cut(s) 393
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 55, 665
Sse9I AATT 4 cut(s) 19, 268, 291, 735
SsiI CCGC 6 cut(s) 344, 375, 477, 479, 488, 707
SspMI CTAG 4 cut(s) 213, 326, 593, 764
StyD4I CCNGG 3 cut(s) 240, 425, 646
StyI CCWWGG 1 cut(s) 592
TaiI ACGT 2 cut(s) 319, 372
TaqI TCGA 2 cut(s) 338, 723
TasI AATT 4 cut(s) 19, 268, 291, 735
TatI WGTACW 1 cut(s) 101
TauI GCSGC 4 cut(s) 377, 479, 482, 491
TfiI GAWTC 3 cut(s) 139, 209, 578
Tru1I TTAA 3 cut(s) 195, 734, 793
Tru9I TTAA 3 cut(s) 195, 734, 793
TseFI GTSAC 3 cut(s) 161, 312, 447
TseI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
Tsp45I GTSAC 3 cut(s) 161, 312, 447
TspDTI ATGAA 2 cut(s) 491, 649
TspGWI ACGGA 1 cut(s) 66
Van91I CCANNNNNTGG 2 cut(s) 370, 716
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 1 cut(s) 291
XbaI TCTAGA 1 cut(s) 212
XmaJI CCTAGG 1 cut(s) 592
XspI CTAG 4 cut(s) 213, 326, 593, 764
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.