MD09G1206700.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016370
Physical Location & Seq
Reverse (-)
19811931 .. 19812656
726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1206700.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGCCCGAGAACCAAACATTTCAGAAGCTGAAAGCACCAGCAACTCATCGTCATGTTCGTCTGGATTGTTGCAGACTCGCCCGAATTCAACCGCCAACACACTCAACACCAAAACCAAGAAGCCCAGCCGAGAGCAGCAGCAAGTAGTACTCAAAAGAAAGAGAGATGATGACAGCAACAACAACAGCAGCAAACACCCAGTTTACAGAGGAGTTCGTAAGCGCAACTGGGGAAAATGGGTGTCGGAAATTCGCGAGCCGCGCAAGAAATCAAGAATCTGGCTGGGCACATTCTCAACGCCTGAGATGGCGGCCCGTGCGCATGACGTGGCCGCCCTCAGCATTAAGGGCAACTCGGCCATTCTGAACTTTCCAGAACTGACCGAGTTGTTGCCACGGCCAGCGTCCCTTACACCTCAAGATATCCAGGCTGCCGCGACTAAAGCAGCAGCCATGGTTAATTTAAATTCTACAACATCATCATCGTTATCATCGTCGTCGTCCTCATCTTCCGTCTTGTTGTCCCAGTCCAAGGGTTCGGACTCTGAGGAGCTAGGGGAGATTGTGGAGTTACCCAATATCGAGGGCAGTTTCGACTCGCCCGAGTCGAGGAACGAGTTCATACTGGTGAATGACTCATTGGATGGGTGGGTGTATCCATCATTGGGGCTCGAGGGAGTTGAGTTAAACTTGTGGGGATTTTTCTCATCAGATAGTAGCAGCTGA

Protein Analysis

242

Amino Acids

26.22

Weight (kDa)

6.2

Isoelectric Point (pI)

65.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 67 - 117 2.2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 321
AccII CGCG 3 cut(s) 255, 262, 437
AciI CCGC 5 cut(s) 93, 260, 311, 333, 435
AcoI YGGCCR 3 cut(s) 330, 357, 398
AcsI RAATTY 3 cut(s) 85, 249, 466
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 3 cut(s) 532, 609, 665
AgsI TTSAA 1 cut(s) 90
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AluBI AGCT 3 cut(s) 29, 553, 723
AluI AGCT 3 cut(s) 29, 553, 723
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 3 cut(s) 6, 602, 671
AoxI GGCC 5 cut(s) 3, 312, 330, 357, 398
ApeKI GCWGC 7 cut(s) 136, 139, 189, 431, 446, 449, 720
ApoI RAATTY 3 cut(s) 85, 249, 466
Asp700I GAANNNNTTC 1 cut(s) 617
AspLEI GCGC 3 cut(s) 225, 264, 322
AspS9I GGNCC 2 cut(s) 4, 313
AsuHPI GGTGA 1 cut(s) 640
AvaI CYCGRG 3 cut(s) 6, 602, 671
BaeGI GKGCMC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 672
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 6 cut(s) 148, 151, 201, 418, 458, 461
BccI CCATC 3 cut(s) 301, 638, 667
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 1 cut(s) 428
BciVI GTATCC 1 cut(s) 666
BfaI CTAG 1 cut(s) 554
BfuI GTATCC 1 cut(s) 666
BmcAI AGTACT 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 428
BmeT110I CYCGRG 3 cut(s) 6, 602, 671
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 2 cut(s) 4, 313
BmrFI CCNGG 1 cut(s) 428
BmrI ACTGGG 3 cut(s) 194, 238, 520
BmuI ACTGGG 3 cut(s) 194, 238, 520
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 395, 453, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 609, 665
Bse1I ACTGG 4 cut(s) 200, 233, 526, 630
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 3 cut(s) 395, 453, 531
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 3 cut(s) 532, 609, 665
BseMII CTCAG 3 cut(s) 294, 352, 537
BseNI ACTGG 4 cut(s) 200, 233, 526, 630
BseRI GAGGAG 2 cut(s) 225, 563
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 6 cut(s) 148, 151, 201, 418, 458, 461
BseYI CCCAGC 2 cut(s) 125, 283
Bsh1236I CGCG 3 cut(s) 255, 262, 437
BshFI GGCC 5 cut(s) 5, 314, 332, 359, 400
BsiHKCI CYCGRG 3 cut(s) 6, 602, 671
BslFI GGGAC 2 cut(s) 391, 508
BslI CCNNNNNNNGG 3 cut(s) 532, 609, 665
BsmFI GGGAC 2 cut(s) 391, 508
BsnI GGCC 5 cut(s) 5, 314, 332, 359, 400
BsoBI CYCGRG 3 cut(s) 6, 602, 671
Bsp1286I GDGCHC 2 cut(s) 290, 672
Bsp19I CCATGG 1 cut(s) 453
Bsp68I TCGCGA 1 cut(s) 255
BspACI CCGC 5 cut(s) 93, 260, 311, 333, 435
BspANI GGCC 5 cut(s) 5, 314, 332, 359, 400
BspCNI CTCAG 3 cut(s) 295, 351, 538
BspFNI CGCG 3 cut(s) 255, 262, 437
BsrI ACTGG 4 cut(s) 200, 233, 526, 630
BssECI CCNNGG 3 cut(s) 395, 453, 531
BssT1I CCWWGG 2 cut(s) 453, 531
Bst2UI CCWGG 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 257, 402
BstDEI CTNAG 3 cut(s) 303, 338, 546
BstDSI CCRYGG 2 cut(s) 395, 453
BstF5I GGATG 1 cut(s) 649
BstFNI CGCG 3 cut(s) 255, 262, 437
BstHHI GCGC 3 cut(s) 225, 264, 322
BstMWI GCNNNNNNNGC 4 cut(s) 261, 317, 348, 443
BstNI CCWGG 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 3 cut(s) 255, 262, 437
BstV1I GCAGC 6 cut(s) 148, 151, 201, 418, 458, 461
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 5 cut(s) 5, 314, 332, 359, 400
BtgI CCRYGG 2 cut(s) 395, 453
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 649
BtuMI TCGCGA 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 257, 402
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 3 cut(s) 225, 264, 322
Cfr13I GGNCC 2 cut(s) 4, 313
CseI GACGC 1 cut(s) 393
Csp6I GTAC 1 cut(s) 149
CviAII CATG 3 cut(s) 54, 323, 454
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 3 cut(s) 303, 338, 546
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 3 cut(s) 330, 357, 398
Eco130I CCWWGG 2 cut(s) 453, 531
Eco24I GRGCYC 1 cut(s) 672
Eco32I GATATC 1 cut(s) 424
Eco88I CYCGRG 3 cut(s) 6, 602, 671
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 453, 531
EcoT38I GRGCYC 1 cut(s) 672
ErhI CCWWGG 2 cut(s) 453, 531
FaeI CATG 3 cut(s) 57, 326, 457
FaiI YATR 4 cut(s) 55, 324, 455, 623
FaqI GGGAC 2 cut(s) 391, 508
FatI CATG 3 cut(s) 53, 322, 453
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 1 cut(s) 672
FspAI RTGCGCAY 1 cut(s) 321
FspBI CTAG 1 cut(s) 554
FspI TGCGCA 1 cut(s) 321
GlaI GCGC 3 cut(s) 224, 263, 321
GsaI CCCAGC 2 cut(s) 129, 287
HaeIII GGCC 5 cut(s) 5, 314, 332, 359, 400
HgaI GACGC 1 cut(s) 393
HhaI GCGC 3 cut(s) 225, 264, 322
Hin1II CATG 3 cut(s) 57, 326, 457
Hin6I GCGC 3 cut(s) 223, 262, 320
HinP1I GCGC 3 cut(s) 223, 262, 320
HinfI GANTC 6 cut(s) 76, 276, 542, 596, 605, 635
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 6 cut(s) 25, 247, 366, 541, 547, 712
Hpy188III TCNNGA 5 cut(s) 63, 254, 273, 374, 419
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 2 cut(s) 499, 502
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 317, 348, 443
HpyF3I CTNAG 3 cut(s) 303, 338, 546
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 3 cut(s) 57, 326, 457
HspAI GCGC 3 cut(s) 223, 262, 320
LmnI GCTCC 1 cut(s) 550
Lsp1109I GCAGC 6 cut(s) 148, 151, 201, 418, 458, 461
MaeI CTAG 1 cut(s) 554
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 570
MboII GAAGA 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 290, 672
MluCI AATT 4 cut(s) 85, 249, 460, 466
MlyI GAGTC 5 cut(s) 70, 536, 590, 614, 629
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 203, 347, 426, 514, 541, 577, 603, 667
MroXI GAANNNNTTC 1 cut(s) 617
MseI TTAA 4 cut(s) 345, 459, 464, 686
MslI CAYNNNNRTG 2 cut(s) 52, 626
MspA1I CMGCKG 1 cut(s) 723
MspR9I CCNGG 1 cut(s) 428
MvaI CCWGG 1 cut(s) 428
MvnI CGCG 3 cut(s) 255, 262, 437
MwoI GCNNNNNNNGC 4 cut(s) 261, 317, 348, 443
NcoI CCATGG 1 cut(s) 453
NlaIII CATG 3 cut(s) 57, 326, 457
NmeAIII GCCGAG 2 cut(s) 155, 335
NruI TCGCGA 1 cut(s) 255
NsbI TGCGCA 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 671
PcsI WCGNNNNNNNCGW 4 cut(s) 56, 491, 600, 605
PdmI GAANNNNTTC 1 cut(s) 617
PfeI GAWTC 1 cut(s) 276
PleI GAGTC 5 cut(s) 70, 536, 590, 613, 629
PpsI GAGTC 5 cut(s) 70, 536, 590, 613, 629
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 2 cut(s) 125, 283
PspGI CCWGG 1 cut(s) 426
PspPI GGNCC 2 cut(s) 4, 313
PspXI VCTCGAGB 1 cut(s) 671
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 723
RruI TCGCGA 1 cut(s) 255
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 2 cut(s) 52, 626
SaqAI TTAA 4 cut(s) 345, 459, 464, 686
Sau96I GGNCC 2 cut(s) 4, 313
ScaI AGTACT 1 cut(s) 150
SchI GAGTC 5 cut(s) 70, 536, 590, 614, 629
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 2 cut(s) 290, 672
SetI ASST 5 cut(s) 31, 330, 418, 555, 725
Sfr274I CTCGAG 1 cut(s) 671
SlaI CTCGAG 1 cut(s) 671
SmiI ATTTAAAT 1 cut(s) 465
SmiMI CAYNNNNRTG 2 cut(s) 52, 626
SmlI CTYRAG 2 cut(s) 417, 671
SmoI CTYRAG 2 cut(s) 417, 671
Sse9I AATT 4 cut(s) 85, 249, 460, 466
SsiI CCGC 5 cut(s) 93, 260, 311, 333, 435
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 2 cut(s) 453, 531
SwaI ATTTAAAT 1 cut(s) 465
TaiI ACGT 1 cut(s) 330
TaqI TCGA 4 cut(s) 582, 594, 608, 672
TaqII GACCGA 1 cut(s) 398
TasI AATT 4 cut(s) 85, 249, 460, 466
TatI WGTACW 1 cut(s) 148
TauI GCSGC 4 cut(s) 262, 314, 335, 437
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 4 cut(s) 345, 459, 464, 686
Tru9I TTAA 4 cut(s) 345, 459, 464, 686
TseI GCWGC 7 cut(s) 136, 139, 189, 431, 446, 449, 720
TspDTI ATGAA 1 cut(s) 610
TspGWI ACGGA 1 cut(s) 502
XapI RAATTY 3 cut(s) 85, 249, 466
XhoI CTCGAG 1 cut(s) 671
XmnI GAANNNNTTC 1 cut(s) 617
XspI CTAG 1 cut(s) 554
ZrmI AGTACT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.