RchiOBHm_Chr2g0139661

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016370
Physical Location & Seq
Forward (+)
57180283 .. 57181323
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51019

Sequence Viewer

Length: 789 bp
ATGGCAGCAGAACCAAACAATTCAGAAGCTGAAAGCACGACAAGCAACTCATCTCTATGTTCAGTAGCCTCTTCTCCGTGTTTATCTGGGTTGTTGCAGAGTACTACTCATTCCAACTCACCCTCCAACACTACCAAGAATCCCAAAACAAGTCAGAAAGGTGACTCCAGCTCAGAGCAGCAGCAGAAGTCGCTTAAAAGAGGAAGAGTATCTAGAGATGATAATAACAGCAGCAAGCACCCGGTTTACAGAGGAGTACGAATGCGCAATTGGGGCAAATGGGTGTCCGAAATTCGCGAGCCTCGCAAGAAGTCACGTATCTGGCTAGGCACATTTTCGACAGCGGAAATGGCAGCCCGTGCCCACGACGTGGCCGCCCTGAGCATCAAGGGCAACTCGGCCATTCTAAACTTCCCAGAACTGGCCGGGTTGCTGCCCCGTCCGGAGTCACTCAAACCTCCGGACATTCAAGCTGCCGCGGCCAAAGCGGCAGCTATGGTTCATCTGAGTTCTACCATCACAACATCATCGTCGTCCACATCCTCATCCTCTTCCTCGTCAGAGGGTTTGGAATCCGAAGAGGACCTAGGCGAGATTGTGGAGTTACCCAACATAGAAGACAGCTTCAACTCCGGCGAGCCGGGAAGCGAGTTCATACTGGTGAACGACTCGGTGGATATAGCGTGGCTGTATGGTCATCCGCCATTATTTGGGCTCGATCACAACTTAATTCCAAGCATTACCTTTGAAGAAGATCTAGTGTCTTGGGATATTCAAATGATTACTTAA

Protein Analysis

262

Amino Acids

28.22

Weight (kDa)

5.2

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 81 - 131 5.3e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g32030
malus_domestica MD09G1206700.v1.1 MD17G1187300.v1.1
prunus_persica Prupe.3G062800_v2.0.a1
pyrus_communis pycom09g12310 pycom17g19630
rosa_chinensis RchiOBHm_Chr2g0139661
rosa_laevigata RLG00000019824
rosa_multiflora Rmu_sc0009449.1_g000010 Rmu_sc0011630.1_g000003
rosa_roxburghii Rroxscaffold_2G00105300
rosa_rugosa Rorug02G0358900
rosa_samantha Rh2AG408900 Rh2CG394800
rosa_wichuraiana Rw2G033480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 266
AccB7I CCANNNNNTGG 2 cut(s) 370, 710
AccII CGCG 2 cut(s) 297, 479
AccIII TCCGGA 2 cut(s) 442, 460
AciI CCGC 6 cut(s) 344, 375, 477, 479, 488, 701
AcoI YGGCCR 4 cut(s) 372, 399, 423, 480
AcsI RAATTY 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 103, 258
AfiI CCNNNNNNNGG 3 cut(s) 370, 421, 710
AgsI TTSAA 4 cut(s) 470, 628, 749, 776
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 5 cut(s) 29, 171, 473, 494, 624
AluI AGCT 5 cut(s) 29, 171, 473, 494, 624
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 2 cut(s) 442, 460
AoxI GGCC 4 cut(s) 372, 399, 423, 480
ApeKI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
ApoI RAATTY 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 586
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 583
AsuC2I CCSGG 3 cut(s) 242, 427, 642
AsuHPI GGTGA 3 cut(s) 111, 173, 673
AvaII GGWCC 1 cut(s) 583
AvrII CCTAGG 1 cut(s) 586
BaeGI GKGCMC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 624
BbvI GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
BccI CCATC 1 cut(s) 524
BcnI CCSGG 3 cut(s) 242, 427, 642
BfaI CTAG 4 cut(s) 213, 326, 587, 758
BglI GCCNNNNNGGC 1 cut(s) 488
BglII AGATCT 1 cut(s) 754
BlnI CCTAGG 1 cut(s) 586
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 3 cut(s) 242, 427, 642
Bme18I GGWCC 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 583
BmrFI CCNGG 3 cut(s) 242, 427, 642
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 624
BplI GAGNNNNNCTC 2 cut(s) 91, 123
BpmI CTGGAG 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 242, 427, 642
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 477, 586
BsaWI WCCGGW 2 cut(s) 442, 460
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 421, 710
Bse1I ACTGG 2 cut(s) 426, 663
BseAI TCCGGA 2 cut(s) 442, 460
BseDI CCNNGG 2 cut(s) 477, 586
BseGI GGATG 3 cut(s) 539, 545, 697
BseLI CCNNNNNNNGG 3 cut(s) 370, 421, 710
BseMII CTCAG 3 cut(s) 186, 371, 497
BseNI ACTGG 2 cut(s) 426, 663
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
Bsh1236I CGCG 2 cut(s) 297, 479
BshFI GGCC 4 cut(s) 374, 401, 425, 482
BsiSI CCGG 6 cut(s) 242, 426, 443, 461, 633, 641
BslI CCNNNNNNNGG 3 cut(s) 370, 421, 710
BsmI GAATGC 1 cut(s) 267
BsnI GGCC 4 cut(s) 374, 401, 425, 482
Bsp1286I GDGCHC 2 cut(s) 364, 717
Bsp13I TCCGGA 2 cut(s) 442, 460
Bsp143I GATC 2 cut(s) 718, 754
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 6 cut(s) 344, 375, 477, 479, 488, 701
BspANI GGCC 4 cut(s) 374, 401, 425, 482
BspCNI CTCAG 3 cut(s) 185, 372, 498
BspEI TCCGGA 2 cut(s) 442, 460
BspFNI CGCG 2 cut(s) 297, 479
BsrI ACTGG 2 cut(s) 426, 663
BssECI CCNNGG 2 cut(s) 477, 586
BssMI GATC 2 cut(s) 718, 754
BssT1I CCWWGG 1 cut(s) 586
Bst6I CTCTTC 4 cut(s) 76, 199, 556, 573
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 236, 299, 638
BstDEI CTNAG 3 cut(s) 172, 380, 506
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 3 cut(s) 539, 545, 697
BstFNI CGCG 2 cut(s) 297, 479
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 2 cut(s) 721, 757
BstMBI GATC 2 cut(s) 718, 754
BstSCI CCNGG 3 cut(s) 240, 425, 640
BstSLI GKGCMC 1 cut(s) 364
BstUI CGCG 2 cut(s) 297, 479
BstV1I GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
BsuRI GGCC 4 cut(s) 374, 401, 425, 482
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 539, 545, 697
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 236, 299, 638
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 583
Cfr42I CCGCGG 1 cut(s) 480
Csp6I GTAC 2 cut(s) 102, 257
CviQI GTAC 2 cut(s) 102, 257
DdeI CTNAG 3 cut(s) 172, 380, 506
DpnI GATC 2 cut(s) 720, 756
DpnII GATC 2 cut(s) 718, 754
DraIII CACNNNGTG 1 cut(s) 370
EaeI YGGCCR 4 cut(s) 372, 399, 423, 480
Eam1104I CTCTTC 4 cut(s) 76, 199, 556, 573
EarI CTCTTC 4 cut(s) 76, 199, 556, 573
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 717
Eco47I GGWCC 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 586
FaiI YATR 6 cut(s) 58, 497, 614, 656, 680, 693
FokI GGATG 3 cut(s) 526, 532, 684
FriOI GRGCYC 1 cut(s) 717
FspBI CTAG 4 cut(s) 213, 326, 587, 758
FspI TGCGCA 1 cut(s) 266
GlaI GCGC 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 4 cut(s) 374, 401, 425, 482
HapII CCGG 6 cut(s) 242, 426, 443, 461, 633, 641
HhaI GCGC 1 cut(s) 267
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 5 cut(s) 139, 164, 446, 572, 668
HpaII CCGG 6 cut(s) 242, 426, 443, 461, 633, 641
HphI GGTGA 3 cut(s) 111, 173, 673
Hpy166II GTNNAC 3 cut(s) 247, 537, 664
Hpy188I TCNGA 7 cut(s) 25, 156, 175, 289, 507, 562, 577
Hpy188III TCNNGA 4 cut(s) 213, 296, 443, 461
Hpy8I GTNNAC 3 cut(s) 247, 537, 664
Hpy99I CGWCG 2 cut(s) 371, 535
HpyCH4IV ACGT 2 cut(s) 316, 369
HpyCH4V TGCA 1 cut(s) 97
HpyF3I CTNAG 3 cut(s) 172, 380, 506
HpySE526I ACGT 2 cut(s) 316, 369
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 2 cut(s) 442, 460
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 2 cut(s) 718, 754
Lsp1109I GCAGC 8 cut(s) 17, 190, 193, 243, 365, 420, 460, 503
LweI GCATC 1 cut(s) 393
MaeI CTAG 4 cut(s) 213, 326, 587, 758
MaeII ACGT 2 cut(s) 316, 369
MaeIII GTNAC 4 cut(s) 161, 312, 447, 603
MalI GATC 2 cut(s) 720, 756
MboI GATC 2 cut(s) 718, 754
MboII GAAGA 7 cut(s) 63, 216, 543, 590, 629, 761, 764
MfeI CAATTG 1 cut(s) 268
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 2 cut(s) 364, 717
MluCI AATT 4 cut(s) 19, 268, 291, 729
MlyI GAGTC 3 cut(s) 158, 455, 662
MmeI TCCRAC 2 cut(s) 138, 150
MroI TCCGGA 2 cut(s) 442, 460
MseI TTAA 3 cut(s) 195, 728, 787
MslI CAYNNNNRTG 2 cut(s) 55, 659
MspA1I CMGCKG 2 cut(s) 344, 479
MspI CCGG 6 cut(s) 242, 426, 443, 461, 633, 641
MspR9I CCNGG 3 cut(s) 242, 427, 642
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 267
MvnI CGCG 2 cut(s) 297, 479
NciI CCSGG 3 cut(s) 242, 427, 642
NdeII GATC 2 cut(s) 718, 754
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 3 cut(s) 161, 312, 447
NruI TCGCGA 1 cut(s) 297
NsbI TGCGCA 1 cut(s) 266
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 2 cut(s) 139, 572
PflMI CCANNNNNTGG 2 cut(s) 370, 710
PleI GAGTC 3 cut(s) 158, 454, 662
PpsI GAGTC 3 cut(s) 158, 454, 662
Ppu21I YACGTR 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 583
Psp5II RGGWCCY 1 cut(s) 583
PspPI GGNCC 1 cut(s) 583
PspPPI RGGWCCY 1 cut(s) 583
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 754
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 2 cut(s) 103, 258
RsaNI GTAC 2 cut(s) 102, 257
RseI CAYNNNNRTG 2 cut(s) 55, 659
SacII CCGCGG 1 cut(s) 480
SaqAI TTAA 3 cut(s) 195, 728, 787
Sau3AI GATC 2 cut(s) 718, 754
Sau96I GGNCC 1 cut(s) 583
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 3 cut(s) 158, 455, 662
ScrFI CCNGG 3 cut(s) 242, 427, 642
SduI GDGCHC 2 cut(s) 364, 717
SfaNI GCATC 1 cut(s) 393
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 1 cut(s) 583
SmiMI CAYNNNNRTG 2 cut(s) 55, 659
Sse9I AATT 4 cut(s) 19, 268, 291, 729
SsiI CCGC 6 cut(s) 344, 375, 477, 479, 488, 701
SspMI CTAG 4 cut(s) 213, 326, 587, 758
StyD4I CCNGG 3 cut(s) 240, 425, 640
StyI CCWWGG 1 cut(s) 586
TaiI ACGT 2 cut(s) 319, 372
TaqI TCGA 2 cut(s) 338, 717
TasI AATT 4 cut(s) 19, 268, 291, 729
TatI WGTACW 1 cut(s) 101
TauI GCSGC 4 cut(s) 377, 479, 482, 491
TfiI GAWTC 2 cut(s) 139, 572
Tru1I TTAA 3 cut(s) 195, 728, 787
Tru9I TTAA 3 cut(s) 195, 728, 787
TseFI GTSAC 3 cut(s) 161, 312, 447
TseI GCWGC 8 cut(s) 5, 178, 181, 231, 353, 433, 473, 491
Tsp45I GTSAC 3 cut(s) 161, 312, 447
TspDTI ATGAA 2 cut(s) 491, 643
TspGWI ACGGA 1 cut(s) 66
Van91I CCANNNNNTGG 2 cut(s) 370, 710
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 1 cut(s) 291
XbaI TCTAGA 1 cut(s) 212
XmaJI CCTAGG 1 cut(s) 586
XspI CTAG 4 cut(s) 213, 326, 587, 758
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.