FvH4_6g32140

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016703
Physical Location & Seq
Reverse (-)
25234629 .. 25235588
960 bp
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UTR
Exon/CDS
Intron
FvH4_6g32140.t1

Sequence Viewer

Length: 558 bp
ATGGCTGCTTCTTTCGTGGGGTCTTTCTTGATGATGCTTTTCTTGGCGCTTGTTGTTGTAAACTCTTCTGCTACGGAATTCCAAGTTGGTGATGATTTTGGTTGGCAGCAGCCTGGCTCAAACAACTCCGCTGTCTACTCCGACTGGGCTACCAAGAACAGGTTTCATGTTGGAGATTCACTCTCTTTCAAGTACAAGAATGATTCAGTTCTTGAGGTAGACAAATGGGGATATTACCACTGCGACACAAGGAATCCGATAATCGCATTTGATAACGGGAAGAGCATAATGAAGCTTGATAGGTCTGGCCCCTTCTACTTCATCAGTGGAGTCCCAGATCACTGCAAGAATGGCCAGCGACTGGTGGTTGAAGTGATGGAACCTCACCCGATTTCGCAATCTCCACTGTCCAGTGCCGACTCACCAGAAGCCCCTTTGGCCGCAGACTCTCCTGCTGCATCCTCAACAAGTTTAGGAGTTGTGATCTCAATTGCACCTTGTTCACTTGTTATTGCTCTTGTTGTCACAAGTTTAGTTTTAGTGTGTTCAGCGCCATAG

Protein Analysis

186

Amino Acids

19.87

Weight (kDa)

4.99

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 42 - 119 5.5e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79800
fragaria_vesca FvH4_6g32140
malus_domestica MD09G1206100.v1.1
prunus_persica Prupe.3G061700_v2.0.a1 Prupe.3G062100_v2.0.a1
pyrus_communis pycom09g12290
rosa_laevigata RLG00000019843
rosa_roxburghii Rroxscaffold_2G00105130
rosa_rugosa Rorug02G0360500
rosa_samantha Rh2AG411200 Rh2BG421400 Rh2CG397300 Rh2DG430900
rosa_wichuraiana Rw2G033680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 361
AccI GTMKAC 2 cut(s) 135, 219
AciI CCGC 2 cut(s) 129, 441
AcoI YGGCCR 2 cut(s) 352, 438
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 159, 361
AgsI TTSAA 2 cut(s) 190, 371
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
AlwNI CAGNNNCTG 1 cut(s) 361
AoxI GGCC 3 cut(s) 307, 352, 438
ApeKI GCWGC 4 cut(s) 5, 106, 109, 455
ApoI RAATTY 1 cut(s) 77
AspLEI GCGC 2 cut(s) 49, 553
AspS9I GGNCC 1 cut(s) 308
AsuHPI GGTGA 3 cut(s) 101, 377, 414
BalI TGGCCA 1 cut(s) 354
BbvI GCAGC 3 cut(s) 118, 121, 442
BccI CCATC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 114
BfoI RGCGCY 2 cut(s) 50, 554
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 5 cut(s) 6, 107, 110, 441, 456
BlsI GCNGC 5 cut(s) 7, 108, 111, 442, 457
Bme1390I CCNGG 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 310, 381
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 24, 467
BmuI ACTGGG 1 cut(s) 154
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpuEI CTTGAG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 361
Bse1I ACTGG 3 cut(s) 149, 366, 411
BseBI CCWGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 2 cut(s) 159, 361
BseNI ACTGG 3 cut(s) 149, 366, 411
BseXI GCAGC 3 cut(s) 118, 121, 442
BshFI GGCC 3 cut(s) 309, 354, 440
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 159, 361
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 3 cut(s) 309, 354, 440
Bsp143I GATC 2 cut(s) 337, 483
BspACI CCGC 2 cut(s) 129, 441
BspANI GGCC 3 cut(s) 309, 354, 440
BspLI GGNNCC 2 cut(s) 310, 381
BspQI GCTCTTC 1 cut(s) 275
BsrI ACTGG 3 cut(s) 149, 366, 411
BssMI GATC 2 cut(s) 337, 483
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 408
Bst6I CTCTTC 2 cut(s) 70, 275
BstC8I GCNNGC 1 cut(s) 356
BstF5I GGATG 1 cut(s) 458
BstH2I RGCGCY 2 cut(s) 50, 554
BstHHI GCGC 2 cut(s) 49, 553
BstKTI GATC 2 cut(s) 340, 486
BstMBI GATC 2 cut(s) 337, 483
BstMWI GCNNNNNNNGC 2 cut(s) 351, 437
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 118, 121, 442
BsuRI GGCC 3 cut(s) 309, 354, 440
BtsCI GGATG 1 cut(s) 458
BtsI GCAGTG 2 cut(s) 238, 340
BtsIMutI CAGTG 5 cut(s) 238, 331, 340, 404, 418
Cac8I GCNNGC 1 cut(s) 356
CaiI CAGNNNCTG 1 cut(s) 361
CfoI GCGC 2 cut(s) 49, 553
Cfr13I GGNCC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 167
CviJI RGCY 9 cut(s) 5, 112, 117, 149, 295, 309, 354, 431, 440
CviKI_1 RGCY 9 cut(s) 5, 112, 117, 149, 295, 309, 354, 431, 440
CviQI GTAC 1 cut(s) 193
DpnI GATC 2 cut(s) 339, 485
DpnII GATC 2 cut(s) 337, 483
EaeI YGGCCR 2 cut(s) 352, 438
Eam1104I CTCTTC 2 cut(s) 70, 275
EarI CTCTTC 2 cut(s) 70, 275
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 1 cut(s) 170
FaiI YATR 3 cut(s) 168, 287, 556
FaqI GGGAC 1 cut(s) 317
FatI CATG 1 cut(s) 166
FblI GTMKAC 2 cut(s) 135, 219
Fnu4HI GCNGC 5 cut(s) 6, 107, 110, 441, 456
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 5 cut(s) 6, 107, 110, 441, 456
GlaI GCGC 2 cut(s) 48, 552
GluI GCNGC 5 cut(s) 6, 107, 110, 441, 456
HaeII RGCGCY 2 cut(s) 50, 554
HaeIII GGCC 3 cut(s) 309, 354, 440
HhaI GCGC 2 cut(s) 49, 553
Hin1II CATG 1 cut(s) 170
Hin6I GCGC 2 cut(s) 47, 551
HinP1I GCGC 2 cut(s) 47, 551
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 6 cut(s) 176, 203, 253, 330, 419, 446
HphI GGTGA 3 cut(s) 101, 377, 414
Hpy166II GTNNAC 4 cut(s) 61, 136, 220, 503
Hpy188I TCNGA 2 cut(s) 142, 258
Hpy188III TCNNGA 2 cut(s) 28, 212
Hpy8I GTNNAC 4 cut(s) 61, 136, 220, 503
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 345, 458, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 437
Hsp92II CATG 1 cut(s) 170
HspAI GCGC 2 cut(s) 47, 551
Kzo9I GATC 2 cut(s) 337, 483
LguI GCTCTTC 1 cut(s) 275
Lsp1109I GCAGC 3 cut(s) 118, 121, 442
LweI GCATC 2 cut(s) 24, 467
MaeIII GTNAC 1 cut(s) 523
MalI GATC 2 cut(s) 339, 485
MboI GATC 2 cut(s) 337, 483
MboII GAAGA 2 cut(s) 57, 292
MfeI CAATTG 1 cut(s) 489
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 2 cut(s) 77, 489
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 3 cut(s) 339, 413, 440
MmeI TCCRAC 2 cut(s) 151, 165
MnlI CCTC 3 cut(s) 208, 393, 472
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 2 cut(s) 351, 437
NdeII GATC 2 cut(s) 337, 483
NlaIII CATG 1 cut(s) 170
NlaIV GGNNCC 2 cut(s) 310, 381
NmuCI GTSAC 1 cut(s) 523
PciSI GCTCTTC 1 cut(s) 275
PfeI GAWTC 3 cut(s) 176, 203, 253
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 5 cut(s) 7, 108, 111, 442, 457
PleI GAGTC 3 cut(s) 338, 413, 440
PpsI GAGTC 3 cut(s) 338, 413, 440
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 310, 381
PspPI GGNCC 1 cut(s) 308
PstNI CAGNNNCTG 1 cut(s) 361
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 275
SatI GCNGC 5 cut(s) 6, 107, 110, 441, 456
Sau3AI GATC 2 cut(s) 337, 483
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 3 cut(s) 339, 413, 440
ScrFI CCNGG 1 cut(s) 114
SetI ASST 6 cut(s) 164, 219, 297, 305, 385, 499
SfaNI GCATC 2 cut(s) 24, 467
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 2 cut(s) 77, 489
SsiI CCGC 2 cut(s) 129, 441
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 408
TasI AATT 2 cut(s) 77, 489
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 3 cut(s) 176, 203, 253
TscAI CASTG 5 cut(s) 245, 331, 347, 411, 418
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 4 cut(s) 5, 106, 109, 455
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 3 cut(s) 155, 305, 310
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 5 cut(s) 245, 331, 347, 411, 418
Van91I CCANNNNNTGG 1 cut(s) 361
XapI RAATTY 1 cut(s) 77
XmiI GTMKAC 2 cut(s) 135, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.