RLG00000019843

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016703
Physical Location & Seq
Reverse (-)
57370387 .. 57371878
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019843

Sequence Viewer

Length: 549 bp
ATGGCTTCTTTCATGGGGTCTGTCTTGACGGTTTTCTTGGCTCTTGTTGTTGTAAACTCTTCTGCTACGGAATTCCAAGTCGGTGATGATTTTGGTTGGCAGCAGCCTGGCTCAAACAACTCCGCTGTCTACTCTAACTGGGCTTCCAACAACAGGTTTCATGTTGGAGATTCACTCTCTTTCAAGTACAAAAATGATTCAGTTCTTGAGGTCGACAAATGGGGCTATTACCACTGCAACACAAGCCATCCCATCATCGCATTTGATAACGGGAAGAGCATAATGAAGCTTGATAGGTCTGGTCCCTTCTACTTCATCAGTGGAGTCCCAGATCATTGCAAAAATGGCCAGCAACTGGTAGTTGAAGTGATGGAACCTCACCCAATTTCACAATCTCCACAGTCCATTGCTGACTCACCAGAACCCCAATTGACTGCAGACTCTCCTGCATCCTCACCAAGTTTAGGAGTTGTGATTTCTATCACACCTTGTTTGCTTGTTATTGCTCTTCTTGTCACAAGTTTAGCTTTAGTATGTTCAGCGCCATAG

Protein Analysis

183

Amino Acids

19.6

Weight (kDa)

4.94

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 117 2.9e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79800
fragaria_vesca FvH4_6g32140
malus_domestica MD09G1206100.v1.1
prunus_persica Prupe.3G061700_v2.0.a1 Prupe.3G062100_v2.0.a1
pyrus_communis pycom09g12290
rosa_laevigata RLG00000019843
rosa_roxburghii Rroxscaffold_2G00105130
rosa_rugosa Rorug02G0360500
rosa_samantha Rh2AG411200 Rh2BG421400 Rh2CG397300 Rh2DG430900
rosa_wichuraiana Rw2G033680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 2 cut(s) 129, 213
AciI CCGC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 1 cut(s) 71
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 3 cut(s) 153, 355, 464
AgsI TTSAA 2 cut(s) 184, 365
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 289, 527
AluI AGCT 2 cut(s) 289, 527
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 100, 103
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 4 cut(s) 95, 371, 408, 447
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 348
BbvI GCAGC 2 cut(s) 112, 115
BccI CCATC 3 cut(s) 255, 260, 364
BciT130I CCWGG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 545
BisI GCNGC 2 cut(s) 101, 104
BlsI GCNGC 2 cut(s) 102, 105
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 304, 375
BmrFI CCNGG 1 cut(s) 108
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 458
BmuI ACTGGG 1 cut(s) 148
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BpuEI CTTGAG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 479
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 355, 464
Bse1I ACTGG 2 cut(s) 143, 360
Bse3DI GCAATG 2 cut(s) 334, 405
Bse8I GATNNNNATC 1 cut(s) 479
BseBI CCWGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 247, 449
BseJI GATNNNNATC 1 cut(s) 479
BseLI CCNNNNNNNGG 3 cut(s) 153, 355, 464
BseMI GCAATG 2 cut(s) 334, 405
BseNI ACTGG 2 cut(s) 143, 360
BseXI GCAGC 2 cut(s) 112, 115
BshFI GGCC 1 cut(s) 348
BslFI GGGAC 2 cut(s) 288, 311
BslI CCNNNNNNNGG 3 cut(s) 153, 355, 464
BsmFI GGGAC 2 cut(s) 288, 311
BsnI GGCC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 331
BspACI CCGC 1 cut(s) 123
BspANI GGCC 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 304, 375
BspMAI CTGCAG 1 cut(s) 439
BspQI GCTCTTC 2 cut(s) 269, 513
BsrDI GCAATG 2 cut(s) 334, 405
BsrI ACTGG 2 cut(s) 143, 360
BssMI GATC 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 31, 402
Bst6I CTCTTC 3 cut(s) 64, 269, 513
BstC8I GCNNGC 1 cut(s) 350
BstF5I GGATG 2 cut(s) 247, 449
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 1 cut(s) 334
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 243, 345
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 112, 115
BsuRI GGCC 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 247, 449
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 325
Cac8I GCNNGC 1 cut(s) 350
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 13, 161
CviQI GTAC 1 cut(s) 187
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 3 cut(s) 64, 269, 513
EarI CTCTTC 3 cut(s) 64, 269, 513
Eco47I GGWCC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 16, 164
FaiI YATR 5 cut(s) 14, 162, 281, 535, 547
FalI AAGNNNNNCTT 2 cut(s) 511, 543
FaqI GGGAC 2 cut(s) 288, 311
FatI CATG 2 cut(s) 12, 160
FblI GTMKAC 2 cut(s) 129, 213
Fnu4HI GCNGC 2 cut(s) 101, 104
FokI GGATG 2 cut(s) 234, 436
Fsp4HI GCNGC 2 cut(s) 101, 104
GlaI GCGC 1 cut(s) 543
GluI GCNGC 2 cut(s) 101, 104
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 1 cut(s) 348
HhaI GCGC 1 cut(s) 544
Hin1II CATG 2 cut(s) 16, 164
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 5 cut(s) 170, 197, 324, 413, 440
HphI GGTGA 4 cut(s) 95, 371, 408, 447
Hpy166II GTNNAC 3 cut(s) 55, 130, 214
Hpy188III TCNNGA 2 cut(s) 25, 206
Hpy8I GTNNAC 3 cut(s) 55, 130, 214
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 31, 402
HpyCH4V TGCA 4 cut(s) 237, 339, 437, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 345
Hsp92II CATG 2 cut(s) 16, 164
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 2 cut(s) 269, 513
Lsp1109I GCAGC 2 cut(s) 112, 115
LweI GCATC 1 cut(s) 458
MaeIII GTNAC 1 cut(s) 514
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 3 cut(s) 51, 286, 500
MfeI CAATTG 1 cut(s) 428
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 3 cut(s) 71, 384, 428
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 3 cut(s) 333, 407, 434
MmeI TCCRAC 2 cut(s) 145, 171
MnlI CCTC 3 cut(s) 202, 387, 463
Mox20I TGGCCA 1 cut(s) 348
MscI TGGCCA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 428
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 243, 345
NdeII GATC 1 cut(s) 331
NlaIII CATG 2 cut(s) 16, 164
NlaIV GGNNCC 2 cut(s) 304, 375
NmuCI GTSAC 1 cut(s) 514
PciSI GCTCTTC 2 cut(s) 269, 513
PfeI GAWTC 2 cut(s) 170, 197
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 2 cut(s) 102, 105
PleI GAGTC 3 cut(s) 332, 407, 434
PpsI GAGTC 3 cut(s) 332, 407, 434
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 304, 375
PspPI GGNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 2 cut(s) 269, 513
SatI GCNGC 2 cut(s) 101, 104
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 3 cut(s) 333, 407, 434
ScrFI CCNGG 1 cut(s) 108
SetI ASST 7 cut(s) 158, 213, 291, 299, 379, 490, 529
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 3 cut(s) 71, 384, 428
SsiI CCGC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 2 cut(s) 31, 402
TaqI TCGA 1 cut(s) 213
TasI AATT 3 cut(s) 71, 384, 428
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 2 cut(s) 170, 197
TscAI CASTG 2 cut(s) 239, 325
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 2 cut(s) 100, 103
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 3 cut(s) 149, 299, 304
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 2 cut(s) 239, 325
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 71
XmiI GTMKAC 2 cut(s) 129, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.