Rh2CG397300

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016703
Physical Location & Seq
Reverse (-)
53995031 .. 53996376
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG397300.1

Sequence Viewer

Length: 549 bp
ATGGCTTCTCTCATGTGGTCTTTCTTGACGGTTTTGTTGGCTCTTGTTGTTGTAAACTCTTCTGCTACGGAATTCCAAGTCGGTGATGATTTTGGTTGGCAGCAGCCTGGCTCAAACAACTCCGCTGTCTACTCTAACTGGGCTTCCAACAACAGGTTTCATGTTGGAGATTCACTCTCTTTCAAGTACAAAAATGATTCAGTTCTTCAGGTCGACAAATGGGGGTATTACCACTGCAACACAAGCCATCCCATCATCGCATTTGAAAATGGAAAGAGCATAATGAAGCTTGATAGATCTGGCCCCTTCTACTTCATCAGTGGAGTCCCAGATCATTGCAAAAATGGCCAGCAACTGGTAGTTGAAGTGATGGAACCTCACCCTATTTCGCAATCTCCACAGTCCATTGCTGACTCACCAGAACCCCATTTGGCTGCAGAATCTCCTGCATCCTCACAAAGTTTAGGAGCTGTGATTTCTATTACACCTTGTTCACTTGTTATTGCTTTTCTTGTCACAAGTTTGGCTTTAGTGTGTTCAGCGCCATAG

Protein Analysis

182

Amino Acids

19.72

Weight (kDa)

5.33

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 117 2.1e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79800
fragaria_vesca FvH4_6g32140
malus_domestica MD09G1206100.v1.1
prunus_persica Prupe.3G061700_v2.0.a1 Prupe.3G062100_v2.0.a1
pyrus_communis pycom09g12290
rosa_laevigata RLG00000019843
rosa_roxburghii Rroxscaffold_2G00105130
rosa_rugosa Rorug02G0360500
rosa_samantha Rh2AG411200 Rh2BG421400 Rh2CG397300 Rh2DG430900
rosa_wichuraiana Rw2G033680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 2 cut(s) 129, 213
AciI CCGC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 191
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 153, 355
AgsI TTSAA 3 cut(s) 184, 266, 365
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 289, 470
AluI AGCT 2 cut(s) 289, 470
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 2 cut(s) 301, 346
ApeKI GCWGC 3 cut(s) 100, 103, 434
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 95, 371, 408
BalI TGGCCA 1 cut(s) 348
BbvI GCAGC 3 cut(s) 112, 115, 421
BccI CCATC 3 cut(s) 255, 260, 364
BciT130I CCWGG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 545
BglII AGATCT 1 cut(s) 296
BisI GCNGC 3 cut(s) 101, 104, 435
BlsI GCNGC 3 cut(s) 102, 105, 436
Bme1390I CCNGG 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 304, 375
BmrFI CCNGG 1 cut(s) 108
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 458
BmuI ACTGGG 1 cut(s) 148
BplI GAGNNNNNCTC 2 cut(s) 159, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 355
Bse1I ACTGG 2 cut(s) 143, 360
Bse3DI GCAATG 2 cut(s) 334, 405
BseBI CCWGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 247, 449
BseLI CCNNNNNNNGG 2 cut(s) 153, 355
BseMI GCAATG 2 cut(s) 334, 405
BseNI ACTGG 2 cut(s) 143, 360
BseXI GCAGC 3 cut(s) 112, 115, 421
BshFI GGCC 2 cut(s) 303, 348
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 153, 355
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 2 cut(s) 303, 348
Bsp143I GATC 2 cut(s) 296, 331
BspACI CCGC 1 cut(s) 123
BspANI GGCC 2 cut(s) 303, 348
BspLI GGNNCC 2 cut(s) 304, 375
BspMAI CTGCAG 1 cut(s) 439
BsrDI GCAATG 2 cut(s) 334, 405
BsrI ACTGG 2 cut(s) 143, 360
BssMI GATC 2 cut(s) 296, 331
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 31, 402
Bst6I CTCTTC 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 350
BstF5I GGATG 2 cut(s) 247, 449
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 2 cut(s) 299, 334
BstMBI GATC 2 cut(s) 296, 331
BstMWI GCNNNNNNNGC 2 cut(s) 243, 345
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 112, 115, 421
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BsuRI GGCC 2 cut(s) 303, 348
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 247, 449
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 325
Cac8I GCNNGC 1 cut(s) 350
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 13, 161
CviQI GTAC 1 cut(s) 187
DpnI GATC 2 cut(s) 298, 333
DpnII GATC 2 cut(s) 296, 331
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 16, 164
FaiI YATR 4 cut(s) 14, 162, 281, 547
FalI AAGNNNNNCTT 2 cut(s) 511, 543
FaqI GGGAC 1 cut(s) 311
FatI CATG 2 cut(s) 12, 160
FblI GTMKAC 2 cut(s) 129, 213
Fnu4HI GCNGC 3 cut(s) 101, 104, 435
FokI GGATG 2 cut(s) 234, 436
Fsp4HI GCNGC 3 cut(s) 101, 104, 435
GlaI GCGC 1 cut(s) 543
GluI GCNGC 3 cut(s) 101, 104, 435
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 2 cut(s) 303, 348
HhaI GCGC 1 cut(s) 544
Hin1II CATG 2 cut(s) 16, 164
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 5 cut(s) 170, 197, 324, 413, 440
HphI GGTGA 3 cut(s) 95, 371, 408
Hpy166II GTNNAC 4 cut(s) 55, 130, 214, 494
Hpy188III TCNNGA 1 cut(s) 25
Hpy8I GTNNAC 4 cut(s) 55, 130, 214, 494
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 2 cut(s) 31, 402
HpyCH4V TGCA 4 cut(s) 237, 339, 437, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 345
Hsp92II CATG 2 cut(s) 16, 164
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 2 cut(s) 296, 331
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 3 cut(s) 112, 115, 421
LweI GCATC 1 cut(s) 458
MaeIII GTNAC 1 cut(s) 514
MalI GATC 2 cut(s) 298, 333
MboI GATC 2 cut(s) 296, 331
MboII GAAGA 2 cut(s) 51, 197
MflI RGATCY 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 1 cut(s) 71
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 2 cut(s) 333, 407
MmeI TCCRAC 2 cut(s) 145, 171
MnlI CCTC 2 cut(s) 387, 463
Mox20I TGGCCA 1 cut(s) 348
MscI TGGCCA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 243, 345
NdeII GATC 2 cut(s) 296, 331
NlaIII CATG 2 cut(s) 16, 164
NlaIV GGNNCC 2 cut(s) 304, 375
NmuCI GTSAC 1 cut(s) 514
PfeI GAWTC 3 cut(s) 170, 197, 440
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 3 cut(s) 102, 105, 436
PleI GAGTC 2 cut(s) 332, 407
PpsI GAGTC 2 cut(s) 332, 407
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 304, 375
PspPI GGNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 296
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SalI GTCGAC 1 cut(s) 212
SatI GCNGC 3 cut(s) 101, 104, 435
Sau3AI GATC 2 cut(s) 296, 331
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 2 cut(s) 333, 407
ScrFI CCNGG 1 cut(s) 108
SetI ASST 6 cut(s) 158, 213, 291, 379, 472, 490
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 435
Sse9I AATT 1 cut(s) 71
SsiI CCGC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 2 cut(s) 31, 402
TaqI TCGA 1 cut(s) 213
TasI AATT 1 cut(s) 71
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 3 cut(s) 170, 197, 440
TscAI CASTG 2 cut(s) 239, 325
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 3 cut(s) 100, 103, 434
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 3 cut(s) 149, 299, 304
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 2 cut(s) 239, 325
Van91I CCANNNNNTGG 1 cut(s) 355
XapI RAATTY 1 cut(s) 71
XmiI GTMKAC 2 cut(s) 129, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.