FvH4_6g36310

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010506
Physical Location & Seq
Forward (+)
28571442 .. 28576409
4968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36310.t1

Sequence Viewer

Length: 1371 bp
ATGAGTAGGCTATCCTTCAGGCCTCGCCCGCTCGACATCCTCAAGAGGCTCCCCTTGGTCAAGTCCTTCAAGGACTTCGAAGACGATGAAGCCCCCTCCTCCACTCGCAACTCTCAGCTCCTCCGTCTCGCCGCCGTCGACTCCGCCTCCGCCACCGAAATCGAGGTCCTCCCTCCCCCCGCCAAGAAGCTAGCTCCAGAAATCCCCACTCCTCAGTTCGTCGTTGTTGATACTTATGAGCGTGACTACTCTCGCACTTTTGCACAGCCTAATTCCTACCTCCGCGCCAGAGGAGCCCGGGCGGAGCTCGGAGAGTTTGTGGAGTATGATTTGGACAATGAGGATGAGGACTGGCTTGAGGAGTTCAACGGTGACGAGGAGATTCTTGCTCCAGAGAAGCTGGAAACTCTTATTTTCAAGCTGGAGGTTTTGGATCACAAAGCGCGGGAGAGAGCAGGAGTTATAACTCCTACTCTTGCTTCACCAATCCCTGTACTTCTACAACTTGATGTCGCTACTGAGGCTCTACAAGGCCCCCTCAGATATGCTGTTATCCGGTCTGTTTACGAGTATTGGAAAGATAAGCGTGAAAGATGGCAGAAGCCTATCTTGCGACGTTTGCAGCCTCCTCCACCGGTTAATGATACCAACCCATACAATGTCTTTAGGCCGAGGGAGAAAACCCACAGACTGCACACAAGAAGGATGCAGAGAAGGGAAAACAATGTGCAATCATTTGAAAAGCTTCGCCAGGTTAGGCGCAACCTGGAGCAAGCCAAGACCATTGTGGAGGCTTTAATCAAGAGAGAAGAAAAAAAGAGGTCAGTCATGGAAGCTGAAGTTGAGCTTCAAGTAAATCAAATGAAGTACAAGCATGAGACTTTGCTCCTGGAAGATAGTTTAATCCTTCCTGGCTTTCCTCCTATGTCTTCCAAGTTTGTTTCAAGTGAGGATGAACTTATTGATTCAGATGACTTCGAAAACAGTCGGCCCCGTACACGACCTTCTGCAATGCCTAATCCGCCTTTACCAGACCCCAACTTGATCATGGTTCCTACGGGAACCATGAAGCAGGATTTTAGGCGTCGGCTTGCACCACAAGGGTGGCTTCATAAAATGGATCCCCTTGAGCCAGTTCTGTTATTTACAAAACCTCTAGTTCCAGAGAAGTTGGCAGCTGCAGGCATTGTACCACCATCAGATTCTTCAACCAAAAATGGAGGATCCACACCACCTCTTAAATTCCGTGGAAGAATTGGCCGAGGGGGGCGATTAATATTTGATAGATGGAACCCATTGATGCACACTCCAATCGACTTGGGGAGTTCATATTATATTCCTCCTAAACCTCGACCAACAACATATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

457

Amino Acids

52.51

Weight (kDa)

8.92

Isoelectric Point (pI)

65.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 47 - 142 6.7e-10 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 138
AccII CGCG 2 cut(s) 285, 445
AciI CCGC 9 cut(s) 29, 132, 144, 150, 180, 283, 302, 445, 1022
AclWI GGATC 5 cut(s) 441, 1115, 1128, 1218, 1231
AcoI YGGCCR 1 cut(s) 1258
AcsI RAATTY 1 cut(s) 1241
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 4 cut(s) 495, 869, 997, 1191
AgeI ACCGGT 1 cut(s) 634
AgsI TTSAA 7 cut(s) 70, 367, 418, 740, 851, 945, 1209
AjnI CCWGG 4 cut(s) 750, 765, 888, 910
AleI CACNNNNGTG 1 cut(s) 1102
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 131, 872
AlwI GGATC 5 cut(s) 441, 1115, 1128, 1218, 1231
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 5 cut(s) 20, 532, 668, 989, 1258
ApeKI GCWGC 3 cut(s) 622, 1175, 1178
ApoI RAATTY 1 cut(s) 1241
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
AseI ATTAAT 1 cut(s) 1274
AsiGI ACCGGT 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 3 cut(s) 287, 445, 762
AspS9I GGNCC 3 cut(s) 166, 533, 990
AsuC2I CCSGG 2 cut(s) 298, 299
AsuHPI GGTGA 2 cut(s) 383, 474
AsuII TTCGAA 2 cut(s) 78, 978
AsuNHI GCTAGC 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 1 cut(s) 166
BamHI GGATCC 2 cut(s) 1120, 1223
BanII GRGCYC 2 cut(s) 298, 309
BarI GAAGNNNNNNTAC 2 cut(s) 988, 1020
BbsI GAAGAC 2 cut(s) 87, 921
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 634, 1165, 1187
BccI CCATC 3 cut(s) 588, 1204, 1281
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 4 cut(s) 752, 767, 890, 912
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 2 cut(s) 298, 299
BcoDI GTCTC 2 cut(s) 131, 872
BfaI CTAG 2 cut(s) 191, 1157
BfmI CTRYAG 1 cut(s) 1179
BisI GCNGC 4 cut(s) 132, 623, 1176, 1179
BlsI GCNGC 4 cut(s) 133, 624, 1177, 1180
Bme1390I CCNGG 6 cut(s) 298, 299, 752, 767, 890, 912
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 297
BmgT120I GGNCC 3 cut(s) 166, 533, 990
BmiI GGNNCC 9 cut(s) 50, 295, 535, 992, 1053, 1063, 1122, 1225, 1292
BmrFI CCNGG 6 cut(s) 298, 299, 752, 767, 890, 912
BmsI GCATC 2 cut(s) 696, 1290
BmtI GCTAGC 1 cut(s) 194
BpiI GAAGAC 2 cut(s) 87, 921
BpmI CTGGAG 4 cut(s) 180, 375, 443, 788
Bpu14I TTCGAA 2 cut(s) 78, 978
BpuEI CTTGAG 3 cut(s) 26, 377, 1148
BpuMI CCSGG 2 cut(s) 298, 299
BsaHI GRCGYC 1 cut(s) 1084
BsaJI CCNNGG 5 cut(s) 54, 297, 671, 1246, 1261
BsaWI WCCGGW 2 cut(s) 555, 634
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 303, 333
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 2 cut(s) 356, 1133
Bse3DI GCAATG 1 cut(s) 1017
BseBI CCWGG 4 cut(s) 752, 767, 890, 912
BseDI CCNNGG 5 cut(s) 54, 297, 671, 1246, 1261
BseGI GGATG 4 cut(s) 36, 349, 711, 958
BseMI GCAATG 1 cut(s) 1017
BseMII CTCAG 4 cut(s) 128, 227, 510, 553
BseNI ACTGG 2 cut(s) 356, 1133
BseRI GAGGAG 7 cut(s) 88, 110, 201, 306, 374, 392, 618
BseXI GCAGC 3 cut(s) 634, 1165, 1187
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 2 cut(s) 285, 445
BshFI GGCC 5 cut(s) 22, 534, 670, 991, 1260
BshTI ACCGGT 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 3 cut(s) 298, 556, 635
BsmAI GTCTC 2 cut(s) 131, 872
BsmBI CGTCTC 1 cut(s) 131
BsnI GGCC 5 cut(s) 22, 534, 670, 991, 1260
BsoBI CYCGRG 1 cut(s) 297
Bsp119I TTCGAA 2 cut(s) 78, 978
Bsp1286I GDGCHC 2 cut(s) 298, 309
Bsp143I GATC 4 cut(s) 433, 1044, 1120, 1223
BspACI CCGC 9 cut(s) 29, 132, 144, 150, 180, 283, 302, 445, 1022
BspANI GGCC 5 cut(s) 22, 534, 670, 991, 1260
BspCNI CTCAG 4 cut(s) 127, 226, 511, 552
BspFNI CGCG 2 cut(s) 285, 445
BspLI GGNNCC 9 cut(s) 50, 295, 535, 992, 1053, 1063, 1122, 1225, 1292
BspMAI CTGCAG 1 cut(s) 1183
BspOI GCTAGC 1 cut(s) 194
BspPI GGATC 5 cut(s) 441, 1115, 1128, 1218, 1231
BspT104I TTCGAA 2 cut(s) 78, 978
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 1017
BsrFI RCCGGY 1 cut(s) 634
BsrI ACTGG 2 cut(s) 356, 1133
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 5 cut(s) 54, 297, 671, 1246, 1261
BssMI GATC 4 cut(s) 433, 1044, 1120, 1223
BssNI GRCGYC 1 cut(s) 1084
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 4 cut(s) 752, 767, 890, 912
Bst4CI ACNGT 2 cut(s) 371, 986
BstACI GRCGYC 1 cut(s) 1084
BstBI TTCGAA 2 cut(s) 78, 978
BstC8I GCNNGC 5 cut(s) 29, 192, 774, 1092, 1183
BstDEI CTNAG 4 cut(s) 114, 213, 519, 539
BstDSI CCRYGG 1 cut(s) 1246
BstF5I GGATG 4 cut(s) 36, 349, 711, 958
BstFNI CGCG 2 cut(s) 285, 445
BstHHI GCGC 3 cut(s) 287, 445, 762
BstKTI GATC 4 cut(s) 436, 1047, 1123, 1226
BstMAI GTCTC 2 cut(s) 131, 872
BstMBI GATC 4 cut(s) 433, 1044, 1120, 1223
BstMWI GCNNNNNNNGC 6 cut(s) 28, 293, 521, 610, 619, 1021
BstNI CCWGG 4 cut(s) 752, 767, 890, 912
BstSCI CCNGG 6 cut(s) 296, 297, 750, 765, 888, 910
BstSFI CTRYAG 1 cut(s) 1179
BstUI CGCG 2 cut(s) 285, 445
BstV1I GCAGC 3 cut(s) 634, 1165, 1187
BstV2I GAAGAC 2 cut(s) 87, 921
BstX2I RGATCY 2 cut(s) 1120, 1223
BstXI CCANNNNNNTGG 1 cut(s) 1104
BstYI RGATCY 2 cut(s) 1120, 1223
BsuRI GGCC 5 cut(s) 22, 534, 670, 991, 1260
BtgI CCRYGG 1 cut(s) 1246
BtsCI GGATG 4 cut(s) 36, 349, 711, 958
Cac8I GCNNGC 5 cut(s) 29, 192, 774, 1092, 1183
CfoI GCGC 3 cut(s) 287, 445, 762
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 3 cut(s) 166, 533, 990
Cfr9I CCCGGG 1 cut(s) 297
CseI GACGC 1 cut(s) 1073
Csp6I GTAC 4 cut(s) 494, 868, 996, 1190
CspAI ACCGGT 1 cut(s) 634
CviAII CATG 4 cut(s) 829, 875, 1048, 1066
CviQI GTAC 4 cut(s) 494, 868, 996, 1190
DdeI CTNAG 4 cut(s) 114, 213, 519, 539
DpnI GATC 4 cut(s) 435, 1046, 1122, 1225
DpnII GATC 4 cut(s) 433, 1044, 1120, 1223
EaeI YGGCCR 1 cut(s) 1258
EciI GGCGGA 4 cut(s) 133, 139, 317, 1011
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 2 cut(s) 298, 309
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 307
EcoO109I RGGNCCY 2 cut(s) 166, 533
EcoRII CCWGG 4 cut(s) 750, 765, 888, 910
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 2 cut(s) 298, 309
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 4 cut(s) 832, 878, 1051, 1069
FalI AAGNNNNNCTT 6 cut(s) 593, 625, 831, 863, 1092, 1124
FatI CATG 4 cut(s) 828, 874, 1047, 1065
FauI CCCGC 3 cut(s) 36, 187, 438
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 132, 623, 1176, 1179
FokI GGATG 4 cut(s) 23, 356, 718, 965
FriOI GRGCYC 2 cut(s) 298, 309
Fsp4HI GCNGC 4 cut(s) 132, 623, 1176, 1179
FspBI CTAG 2 cut(s) 191, 1157
GlaI GCGC 3 cut(s) 286, 444, 761
GluI GCNGC 4 cut(s) 132, 623, 1176, 1179
GsuI CTGGAG 4 cut(s) 180, 375, 443, 788
HaeIII GGCC 5 cut(s) 22, 534, 670, 991, 1260
HapII CCGG 3 cut(s) 298, 556, 635
HgaI GACGC 1 cut(s) 1073
HhaI GCGC 3 cut(s) 287, 445, 762
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 4 cut(s) 832, 878, 1051, 1069
Hin6I GCGC 3 cut(s) 285, 443, 760
HinP1I GCGC 3 cut(s) 285, 443, 760
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 4 cut(s) 140, 382, 965, 1202
HpaII CCGG 3 cut(s) 298, 556, 635
HphI GGTGA 2 cut(s) 383, 474
Hpy166II GTNNAC 3 cut(s) 139, 565, 998
Hpy188I TCNGA 4 cut(s) 311, 542, 970, 1201
Hpy188III TCNNGA 5 cut(s) 43, 197, 392, 802, 1163
Hpy8I GTNNAC 3 cut(s) 139, 565, 998
Hpy99I CGWCG 4 cut(s) 140, 224, 618, 1089
HpyAV CCTTC 6 cut(s) 25, 76, 696, 708, 917, 1014
HpyCH4III ACNGT 2 cut(s) 371, 986
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 9 cut(s) 263, 622, 694, 709, 730, 1010, 1094, 1181, 1303
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 293, 521, 610, 619, 1021
HpyF3I CTNAG 4 cut(s) 114, 213, 519, 539
HpySE526I ACGT 1 cut(s) 616
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 4 cut(s) 832, 878, 1051, 1069
HspAI GCGC 3 cut(s) 285, 443, 760
Ksp22I TGATCA 1 cut(s) 1044
Kzo9I GATC 4 cut(s) 433, 1044, 1120, 1223
LmnI GCTCC 8 cut(s) 54, 123, 199, 293, 304, 394, 769, 891
Lsp1109I GCAGC 3 cut(s) 634, 1165, 1187
LweI GCATC 2 cut(s) 696, 1290
MaeI CTAG 2 cut(s) 191, 1157
MaeII ACGT 1 cut(s) 616
MaeIII GTNAC 2 cut(s) 242, 371
MalI GATC 4 cut(s) 435, 1046, 1122, 1225
MbiI CCGCTC 1 cut(s) 31
MboI GATC 4 cut(s) 433, 1044, 1120, 1223
MboII GAAGA 6 cut(s) 92, 821, 905, 921, 1197, 1263
MflI RGATCY 2 cut(s) 1120, 1223
MhlI GDGCHC 2 cut(s) 298, 309
MluCI AATT 4 cut(s) 271, 1241, 1254, 1366
MlyI GAGTC 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 5 cut(s) 639, 797, 902, 1239, 1274
MslI CAYNNNNRTG 1 cut(s) 1102
MspA1I CMGCKG 1 cut(s) 1178
MspI CCGG 3 cut(s) 298, 556, 635
MspR9I CCNGG 6 cut(s) 298, 299, 752, 767, 890, 912
MvaI CCWGG 4 cut(s) 752, 767, 890, 912
MvnI CGCG 2 cut(s) 285, 445
MwoI GCNNNNNNNGC 6 cut(s) 28, 293, 521, 610, 619, 1021
NciI CCSGG 2 cut(s) 298, 299
NdeII GATC 4 cut(s) 433, 1044, 1120, 1223
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 4 cut(s) 832, 878, 1051, 1069
NlaIV GGNNCC 9 cut(s) 50, 295, 535, 992, 1053, 1063, 1122, 1225, 1292
NmeAIII GCCGAG 2 cut(s) 696, 1286
NmuCI GTSAC 2 cut(s) 242, 371
NspV TTCGAA 2 cut(s) 78, 978
OliI CACNNNNGTG 1 cut(s) 1102
PceI AGGCCT 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 3 cut(s) 382, 965, 1202
PfoI TCCNGGA 1 cut(s) 888
PinAI ACCGGT 1 cut(s) 634
PkrI GCNGC 4 cut(s) 133, 624, 1177, 1180
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 166
PshBI ATTAAT 1 cut(s) 1274
PsiI TTATAA 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 4 cut(s) 750, 765, 888, 910
PspGI CCWGG 4 cut(s) 750, 765, 888, 910
PspN4I GGNNCC 9 cut(s) 50, 295, 535, 992, 1053, 1063, 1122, 1225, 1292
PspPI GGNCC 3 cut(s) 166, 533, 990
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 1183
PsuI RGATCY 2 cut(s) 1120, 1223
PvuII CAGCTG 1 cut(s) 1178
RsaI GTAC 4 cut(s) 495, 869, 997, 1191
RsaNI GTAC 4 cut(s) 494, 868, 996, 1190
RseI CAYNNNNRTG 1 cut(s) 1102
SacI GAGCTC 1 cut(s) 309
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 5 cut(s) 639, 797, 902, 1239, 1274
SatI GCNGC 4 cut(s) 132, 623, 1176, 1179
Sau3AI GATC 4 cut(s) 433, 1044, 1120, 1223
Sau96I GGNCC 3 cut(s) 166, 533, 990
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 6 cut(s) 298, 299, 752, 767, 890, 912
SduI GDGCHC 2 cut(s) 298, 309
SfaNI GCATC 2 cut(s) 696, 1290
SfcI CTRYAG 1 cut(s) 1179
SfuI TTCGAA 2 cut(s) 78, 978
SinI GGWCC 1 cut(s) 166
SmaI CCCGGG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 1102
SmlI CTYRAG 3 cut(s) 41, 356, 1127
SmoI CTYRAG 3 cut(s) 41, 356, 1127
SrfI GCCCGGGC 1 cut(s) 299
Sse9I AATT 4 cut(s) 271, 1241, 1254, 1366
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 9 cut(s) 29, 132, 144, 150, 180, 283, 302, 445, 1022
SspI AATATT 1 cut(s) 1278
SspMI CTAG 2 cut(s) 191, 1157
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 6 cut(s) 296, 297, 750, 765, 888, 910
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 371, 986
TaiI ACGT 1 cut(s) 619
TaqI TCGA 7 cut(s) 33, 78, 138, 162, 978, 1314, 1351
TasI AATT 4 cut(s) 271, 1241, 1254, 1366
TatI WGTACW 2 cut(s) 493, 867
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 382, 965, 1202
Tru1I TTAA 5 cut(s) 639, 797, 902, 1239, 1274
Tru9I TTAA 5 cut(s) 639, 797, 902, 1239, 1274
TseFI GTSAC 2 cut(s) 242, 371
TseI GCWGC 3 cut(s) 622, 1175, 1178
Tsp45I GTSAC 2 cut(s) 242, 371
TspDTI ATGAA 6 cut(s) 102, 878, 969, 1082, 1100, 1317
TspGWI ACGGA 2 cut(s) 113, 1235
TspMI CCCGGG 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 1274
XapI RAATTY 1 cut(s) 1241
XcmI CCANNNNNNNNNTGG 2 cut(s) 784, 1045
XmaI CCCGGG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 2 cut(s) 191, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.