MD09G1170200.v1.1

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010506
Physical Location & Seq
Reverse (-)
14209028 .. 14213404
4377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1170200.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGAGTAGACTTTCATTCAGGCCTCGCCCTGTTGATATCCACAAGAAGCTCCCCATTGTCAAATCCGTCAAGGACTTCGAAGACGATGATACCCCAACTTCCACCCGCAATTCTCAGCTCATCCGCCTCGCCTACGACGCCACCGTTGAACCGGAGGCACCCCCGACCCCCGCCGCGCCCAAGAAATATGTTCCTGAAATACCCACTCCTCAGTTTGTTGTTGTGGACACTTATGAGAGGGACTATTCTCGCACTTTTGGCCAGCCCAATTCGTACCTTCGCGCTAGAGGAGCTCGGGCCGAGCTCGGAGAGTTTGTGGAGTATGACCTAGACAATGAGGATGAGGACTGGCTGTATGAGTTCAACAAAGATAGGAAGATTCTTGCACCCGAAAAGCTTGAGAGTCTTCTTTTTAAGTTGGAGGTTTTGGATCATAAGGCGCGAGAAAGAGCGGGAGTTATAACTCCTACTCTTGCTTCACCAATCCCTGTACTTCTACAACTTGATGTTGCTAGTGAGGCTTTACAAGCTCAGTCTCTCAGATATGCTGTTATCCAGTCTGTTTATAATTATTGGAAAGAGAAGCGTGAACGTTGGCAGAAGCCTGTTTTGCGGCGTTTGCAGCCTCCTCCACCAGTTAATGACACTAACCCGTATAATGTCTTTAGACCAAGGGAGAAAACCCACAGACTGCACACAAGAAGGATGCAAAGAAGGGAAAATAATGTGCAGTCATTTGAAAAGCTTCGCCAGGTTAGGCGCAACCTGGAGCAAGCCAAGACCATAGTAGAGGCTTTAATCAAGAGAGAAGAGAAAAAGAGGGAAGTCATGGAGAATGAAGTTAGTCTTCAAATAATCCAAATGAAATACAAGCATGAAACTGAGCTCCTGGAAGATAGTTTGGCCCTTCCTGGATTTCAACCTGTATCTTGCAAATTTGGTTCAAGCGAGGACGAATTTGTTGATTCGGATGATGTTTCAAACAGCCAGCCCCGTATGCGACCTCCCGCAATACCTAATCCGCCTTTAACAGATTCAAATTTAGTTATGGTTCCTACAGGAACCATGAAGCAAGAGTTCAGGCGGCGGCATGCACCACAAGGGTGGCTTCATAAAATGGATCCCCTTGAGCCAGTTTTGTTATTTACAAGACCTCTAGTTCCAGAAAAGTTGGCAGCAGCAGGCATTGTACCCCCATCAGATTCTTCAGTAACAAATGGAGCATCCACACCACCTATTAAATTCCGAGGAAGAATTGGCCGAGGAGGACGATTGATATTTGATAGATGGAATCCATTGATGCATACTCCAATCGACTGTGGGAATTCTTTTTATATACCGCCTAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

451

Amino Acids

51.91

Weight (kDa)

9.12

Isoelectric Point (pI)

61.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 29 - 129 1.8e-09 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 461, 567
AccB1I GGYRCC 1 cut(s) 157
AccBSI CCGCTC 1 cut(s) 452
AccI GTMKAC 1 cut(s) 7
AccII CGCG 3 cut(s) 176, 282, 442
AclI AACGTT 1 cut(s) 592
AclWI GGATC 3 cut(s) 438, 1115, 1128
AcoI YGGCCR 2 cut(s) 259, 1258
AcsI RAATTY 5 cut(s) 935, 956, 1039, 1241, 1324
AcuI CTGAAG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 3 cut(s) 275, 492, 1191
AgsI TTSAA 8 cut(s) 149, 364, 740, 851, 920, 945, 981, 1038
AjnI CCWGG 4 cut(s) 750, 765, 888, 910
AleI CACNNNNGTG 1 cut(s) 1102
AluBI AGCT 8 cut(s) 49, 118, 293, 304, 397, 530, 745, 886
AluI AGCT 8 cut(s) 49, 118, 293, 304, 397, 530, 745, 886
Alw21I GWGCWC 3 cut(s) 295, 306, 888
Alw26I GTCTC 1 cut(s) 540
AlwI GGATC 3 cut(s) 438, 1115, 1128
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 5 cut(s) 20, 259, 297, 903, 1258
ApeKI GCWGC 3 cut(s) 622, 1175, 1178
ApoI RAATTY 5 cut(s) 935, 956, 1039, 1241, 1324
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 4 cut(s) 178, 284, 442, 762
AspS9I GGNCC 2 cut(s) 297, 904
AsuHPI GGTGA 1 cut(s) 471
AsuII TTCGAA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 294
BalI TGGCCA 1 cut(s) 261
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 3 cut(s) 295, 306, 888
BbsI GAAGAC 3 cut(s) 87, 398, 839
Bbv12I GWGCWC 3 cut(s) 295, 306, 888
BbvI GCAGC 3 cut(s) 634, 1187, 1190
BccI CCATC 2 cut(s) 1204, 1281
BciT130I CCWGG 4 cut(s) 752, 767, 890, 912
BcoDI GTCTC 1 cut(s) 540
BfaI CTAG 4 cut(s) 285, 329, 513, 1157
BfmI CTRYAG 1 cut(s) 1056
BisI GCNGC 7 cut(s) 174, 614, 623, 1085, 1088, 1176, 1179
BlsI GCNGC 7 cut(s) 175, 615, 624, 1086, 1089, 1177, 1180
Bme1390I CCNGG 4 cut(s) 752, 767, 890, 912
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 297, 904
BmiI GGNNCC 4 cut(s) 159, 1053, 1063, 1122
BmrFI CCNGG 4 cut(s) 752, 767, 890, 912
BmsI GCATC 3 cut(s) 696, 1232, 1290
BpiI GAAGAC 3 cut(s) 87, 398, 839
BpmI CTGGAG 1 cut(s) 788
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 419, 1148
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 671, 1246, 1261
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bse1I ACTGG 4 cut(s) 353, 556, 635, 1133
BseBI CCWGG 4 cut(s) 752, 767, 890, 912
BseDI CCNNGG 3 cut(s) 671, 1246, 1261
BseGI GGATG 5 cut(s) 120, 346, 711, 976, 1223
BseMII CTCAG 5 cut(s) 128, 224, 545, 553, 873
BseNI ACTGG 4 cut(s) 353, 556, 635, 1133
BseRI GAGGAG 4 cut(s) 198, 303, 618, 1278
BseXI GCAGC 3 cut(s) 634, 1187, 1190
BsgI GTGCAG 2 cut(s) 677, 749
Bsh1236I CGCG 3 cut(s) 176, 282, 442
BshFI GGCC 5 cut(s) 22, 261, 299, 905, 1260
BshNI GGYRCC 1 cut(s) 157
BsiHKAI GWGCWC 3 cut(s) 295, 306, 888
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 540
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 5 cut(s) 22, 261, 299, 905, 1260
BsoBI CYCGRG 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 3 cut(s) 295, 306, 888
Bsp143I GATC 2 cut(s) 430, 1120
BspANI GGCC 5 cut(s) 22, 261, 299, 905, 1260
BspCNI CTCAG 5 cut(s) 127, 223, 544, 552, 874
BspFNI CGCG 3 cut(s) 176, 282, 442
BspLI GGNNCC 4 cut(s) 159, 1053, 1063, 1122
BspPI GGATC 3 cut(s) 438, 1115, 1128
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 452
BsrI ACTGG 4 cut(s) 353, 556, 635, 1133
BssECI CCNNGG 3 cut(s) 671, 1246, 1261
BssMI GATC 2 cut(s) 430, 1120
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 671
Bst2UI CCWGG 4 cut(s) 752, 767, 890, 912
Bst4CI ACNGT 2 cut(s) 145, 1319
Bst6I CTCTTC 1 cut(s) 804
BstACI GRCGYC 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 5 cut(s) 263, 774, 989, 1092, 1183
BstDEI CTNAG 5 cut(s) 114, 210, 531, 539, 882
BstF5I GGATG 5 cut(s) 120, 346, 711, 976, 1223
BstFNI CGCG 3 cut(s) 176, 282, 442
BstHHI GCGC 4 cut(s) 178, 284, 442, 762
BstKTI GATC 2 cut(s) 433, 1123
BstMAI GTCTC 1 cut(s) 540
BstMBI GATC 2 cut(s) 430, 1120
BstMWI GCNNNNNNNGC 9 cut(s) 137, 258, 290, 518, 527, 610, 619, 622, 997
BstNI CCWGG 4 cut(s) 752, 767, 890, 912
BstNSI RCATGY 1 cut(s) 1094
BstSCI CCNGG 4 cut(s) 750, 765, 888, 910
BstSFI CTRYAG 1 cut(s) 1056
BstUI CGCG 3 cut(s) 176, 282, 442
BstV1I GCAGC 3 cut(s) 634, 1187, 1190
BstV2I GAAGAC 3 cut(s) 87, 398, 839
BstX2I RGATCY 1 cut(s) 1120
BstXI CCANNNNNNTGG 1 cut(s) 1104
BstYI RGATCY 1 cut(s) 1120
BsuRI GGCC 5 cut(s) 22, 261, 299, 905, 1260
BtsCI GGATG 5 cut(s) 120, 346, 711, 976, 1223
Cac8I GCNNGC 5 cut(s) 263, 774, 989, 1092, 1183
CfoI GCGC 4 cut(s) 178, 284, 442, 762
Cfr13I GGNCC 2 cut(s) 297, 904
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 274, 491, 1190
CviAII CATG 4 cut(s) 829, 875, 1066, 1091
CviQI GTAC 3 cut(s) 274, 491, 1190
DdeI CTNAG 5 cut(s) 114, 210, 531, 539, 882
DpnI GATC 2 cut(s) 432, 1122
DpnII GATC 2 cut(s) 430, 1120
EaeI YGGCCR 2 cut(s) 259, 1258
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
EciI GGCGGA 2 cut(s) 113, 1011
Ecl136II GAGCTC 3 cut(s) 293, 304, 886
Eco130I CCWWGG 1 cut(s) 671
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 3 cut(s) 295, 306, 888
Eco32I GATATC 1 cut(s) 37
Eco53kI GAGCTC 3 cut(s) 293, 304, 886
Eco57I CTGAAG 1 cut(s) 1191
Eco88I CYCGRG 1 cut(s) 294
EcoICRI GAGCTC 3 cut(s) 293, 304, 886
EcoRI GAATTC 1 cut(s) 1324
EcoRII CCWGG 4 cut(s) 750, 765, 888, 910
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 671
EcoT22I ATGCAT 1 cut(s) 1305
EcoT38I GRGCYC 3 cut(s) 295, 306, 888
ErhI CCWWGG 1 cut(s) 671
FaeI CATG 4 cut(s) 832, 878, 1069, 1094
FalI AAGNNNNNCTT 4 cut(s) 831, 863, 1092, 1124
FaqI GGGAC 1 cut(s) 254
FatI CATG 4 cut(s) 828, 874, 1065, 1090
FauI CCCGC 4 cut(s) 113, 178, 445, 1015
FblI GTMKAC 1 cut(s) 7
Fnu4HI GCNGC 7 cut(s) 174, 614, 623, 1085, 1088, 1176, 1179
FokI GGATG 5 cut(s) 107, 353, 718, 983, 1210
FriOI GRGCYC 3 cut(s) 295, 306, 888
Fsp4HI GCNGC 7 cut(s) 174, 614, 623, 1085, 1088, 1176, 1179
FspBI CTAG 4 cut(s) 285, 329, 513, 1157
GlaI GCGC 4 cut(s) 177, 283, 441, 761
GluI GCNGC 7 cut(s) 174, 614, 623, 1085, 1088, 1176, 1179
GsuI CTGGAG 1 cut(s) 788
HaeIII GGCC 5 cut(s) 22, 261, 299, 905, 1260
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 146
HhaI GCGC 4 cut(s) 178, 284, 442, 762
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 832, 878, 1069, 1094
Hin6I GCGC 4 cut(s) 176, 282, 440, 760
HinP1I GCGC 4 cut(s) 176, 282, 440, 760
HindIII AAGCTT 2 cut(s) 395, 743
HinfI GANTC 6 cut(s) 379, 403, 965, 1034, 1202, 1291
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 471
Hpy166II GTNNAC 3 cut(s) 8, 226, 590
Hpy188I TCNGA 5 cut(s) 308, 542, 970, 1201, 1247
Hpy188III TCNNGA 3 cut(s) 194, 802, 1163
Hpy8I GTNNAC 3 cut(s) 8, 226, 590
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 4 cut(s) 287, 696, 708, 917
HpyCH4III ACNGT 2 cut(s) 145, 1319
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 8 cut(s) 386, 622, 694, 709, 730, 933, 1094, 1303
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 258, 290, 518, 527, 610, 619, 622, 997
HpyF3I CTNAG 5 cut(s) 114, 210, 531, 539, 882
HpySE526I ACGT 1 cut(s) 592
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 832, 878, 1069, 1094
HspAI GCGC 4 cut(s) 176, 282, 440, 760
Kzo9I GATC 2 cut(s) 430, 1120
LmnI GCTCC 5 cut(s) 54, 290, 769, 891, 1220
Lsp1109I GCAGC 3 cut(s) 634, 1187, 1190
LweI GCATC 3 cut(s) 696, 1232, 1290
MaeI CTAG 4 cut(s) 285, 329, 513, 1157
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 1 cut(s) 1210
MalI GATC 2 cut(s) 432, 1122
MbiI CCGCTC 1 cut(s) 452
MboI GATC 2 cut(s) 430, 1120
MboII GAAGA 8 cut(s) 92, 388, 398, 821, 839, 905, 1197, 1263
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 3 cut(s) 295, 306, 888
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 9 cut(s) 109, 268, 568, 935, 956, 1039, 1241, 1254, 1324
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 399
Mox20I TGGCCA 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 1305
MroXI GAANNNNTTC 1 cut(s) 744
MscI TGGCCA 1 cut(s) 261
MseI TTAA 5 cut(s) 414, 639, 797, 1028, 1239
MslI CAYNNNNRTG 1 cut(s) 1102
Msp20I TGGCCA 1 cut(s) 261
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 4 cut(s) 752, 767, 890, 912
MvaI CCWGG 4 cut(s) 752, 767, 890, 912
MvnI CGCG 3 cut(s) 176, 282, 442
MwoI GCNNNNNNNGC 9 cut(s) 137, 258, 290, 518, 527, 610, 619, 622, 997
NdeII GATC 2 cut(s) 430, 1120
NlaIII CATG 4 cut(s) 832, 878, 1069, 1094
NlaIV GGNNCC 4 cut(s) 159, 1053, 1063, 1122
NmeAIII GCCGAG 2 cut(s) 325, 1286
NsiI ATGCAT 1 cut(s) 1305
NspI RCATGY 1 cut(s) 1094
NspV TTCGAA 1 cut(s) 78
OliI CACNNNNGTG 1 cut(s) 1102
PaeI GCATGC 1 cut(s) 1094
PceI AGGCCT 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 5 cut(s) 379, 965, 1034, 1202, 1291
PfoI TCCNGGA 2 cut(s) 888, 910
PkrI GCNGC 7 cut(s) 175, 615, 624, 1086, 1089, 1177, 1180
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PsiI TTATAA 2 cut(s) 461, 567
Psp124BI GAGCTC 3 cut(s) 295, 306, 888
Psp1406I AACGTT 1 cut(s) 592
Psp6I CCWGG 4 cut(s) 750, 765, 888, 910
PspGI CCWGG 4 cut(s) 750, 765, 888, 910
PspN4I GGNNCC 4 cut(s) 159, 1053, 1063, 1122
PspPI GGNCC 2 cut(s) 297, 904
PsuI RGATCY 1 cut(s) 1120
RsaI GTAC 3 cut(s) 275, 492, 1191
RsaNI GTAC 3 cut(s) 274, 491, 1190
RseI CAYNNNNRTG 1 cut(s) 1102
SacI GAGCTC 3 cut(s) 295, 306, 888
SaqAI TTAA 5 cut(s) 414, 639, 797, 1028, 1239
SatI GCNGC 7 cut(s) 174, 614, 623, 1085, 1088, 1176, 1179
Sau3AI GATC 2 cut(s) 430, 1120
Sau96I GGNCC 2 cut(s) 297, 904
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 4 cut(s) 752, 767, 890, 912
SduI GDGCHC 3 cut(s) 295, 306, 888
SfaNI GCATC 3 cut(s) 696, 1232, 1290
SfcI CTRYAG 1 cut(s) 1056
SfuI TTCGAA 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 1102
SmlI CTYRAG 2 cut(s) 398, 1127
SmoI CTYRAG 2 cut(s) 398, 1127
SphI GCATGC 1 cut(s) 1094
Sse9I AATT 9 cut(s) 109, 268, 568, 935, 956, 1039, 1241, 1254, 1324
SseBI AGGCCT 1 cut(s) 22
SspMI CTAG 4 cut(s) 285, 329, 513, 1157
SstI GAGCTC 3 cut(s) 295, 306, 888
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 4 cut(s) 750, 765, 888, 910
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 2 cut(s) 145, 1319
TaiI ACGT 1 cut(s) 595
TaqI TCGA 2 cut(s) 78, 1314
TasI AATT 9 cut(s) 109, 268, 568, 935, 956, 1039, 1241, 1254, 1324
TatI WGTACW 1 cut(s) 490
TauI GCSGC 4 cut(s) 176, 616, 1087, 1090
TfiI GAWTC 5 cut(s) 379, 965, 1034, 1202, 1291
Tru1I TTAA 5 cut(s) 414, 639, 797, 1028, 1239
Tru9I TTAA 5 cut(s) 414, 639, 797, 1028, 1239
TseI GCWGC 3 cut(s) 622, 1175, 1178
TspDTI ATGAA 5 cut(s) 852, 878, 891, 1082, 1100
TspGWI ACGGA 1 cut(s) 55
XapI RAATTY 5 cut(s) 935, 956, 1039, 1241, 1324
XceI RCATGY 1 cut(s) 1094
XmiI GTMKAC 1 cut(s) 7
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 4 cut(s) 285, 329, 513, 1157
Zsp2I ATGCAT 1 cut(s) 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.