Rh2BG477300

Enhancer of polycomb-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010506
Physical Location & Seq
Forward (+)
67251011 .. 67256034
5024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG477300.1

Sequence Viewer

Length: 1374 bp
ATGAGTAGGCTCTCATTCAGGCCTCGGCCCCTCGACATCCACAAGAGGCTCCCCGTCGTCAAGTCTTTCAAGGACTTCGAAGACGATGAAGCCGCCCCCACCTCCACCCGCAATTCTCAGCTCCTCCGCCTCTCCGCCGTCGACTCCTCCTCCGCCTCCGCCACCGAAATCGAGGTCCACCCTCCCCCCGCCAAGAAGCTAGCCCCTGAAATCCCGACTCCTCAGTTTGTTGTGGTTGATACTTATGAGAGAGACTACTCTCGCACTTTTGCCCAACCCAATTCCTATCTCCGCGCCAGAGGAGCCCGGGCCGAGCTCGGTGAGTTTGTGGAGTATGATTTAGATAATGAGGATGAGGATTGGCTTGAAGAGTTCAATGGTGAGGAGGAAATTCTTCCTGCCGAAAAGCTTGAGAGTCTTATTTTCAAGCTGGAGGTTTTGGATCATAAGGCTCGGGAGAGAGCAGGAGTTATAACCCCTACTCTTGCTTCACCTATCCCTGTACTTCTACAACTTGATGTTGCTACTGAGGCTCTACATGGTCAGCCCCTCAGATATGCTGTCATCCGGTCTGTTTATGAGTATTGGAGAGATAAGCGTGAAAGATGGCAGAAGCCTATCTTGCGACGTTTGCAGCCTCCTCCACCGGTTAATGATACCAACCCATACAATGTCTTCAGGCCAAGGGAGAAAACCCACAGACTGCACACGAGAAGGATGCAAAGAAGGGAAAACAATGTGCAATCATTTGAAAAGCTTCGCCAGGTTAGGCGCAACCTGGACCAAGCCAAGACCATTGTGGAGGCTTTAATCAAGAGAGAAGAAAAAAAGAGAGCAGTCATGGAGGCCGAAGTGGAGCTTCAAGTAAATCAAATGAAGTACAAGCATGAGACACTGCTCCTGGAAGATAGTTTAGTCCTTCCTGGTTTTCCACCCATGTCTTCCAAGTTTGTTTCAAGTGAGGAGGAAAATGTTGATTCAGATGACTTTGAAAACAGTCGGCCTCGTACACGACCTTCTGCACTGCCTAATCCGCCTTTACCAGACCCCAACTTGATCACAGGAACCATGAAGCAAGATTTCAGGCGTCGGCATGCACCTCAAGGGTGGCTTCATAAAATGGATCCCCTCGAGCCAGTTTTGTTATTTACAAAACCCTTAGTTCCAGAGAAGTTGGCAGCTGCAGGCATTGTACCACCATCAGATTCTTCAACGAAAAATGGAGTAACCACAGCACCTTTTAAGTTCCGCGGAAGAATTGGCCGAGGGGGCCGATTAATATTTGATAGATGGAACCCATTGATGCACACTCCAATCGACTTTGGGAATTCATATTATATACCTCCTAAACCTCGACCAACAACATATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

457

Amino Acids

52.74

Weight (kDa)

8.93

Isoelectric Point (pI)

63.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 44 - 145 2.1e-10 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AccI GTMKAC 1 cut(s) 141
AccII CGCG 2 cut(s) 294, 1251
AclWI GGATC 3 cut(s) 450, 1118, 1131
AcoI YGGCCR 1 cut(s) 1261
AcsI RAATTY 2 cut(s) 390, 1327
AcuI CTGAAG 1 cut(s) 661
AcyI GRCGYC 1 cut(s) 1087
AfaI GTAC 4 cut(s) 504, 881, 1009, 1194
AgeI ACCGGT 1 cut(s) 646
AgsI TTSAA 9 cut(s) 70, 368, 376, 427, 752, 863, 957, 992, 1212
AjnI CCWGG 4 cut(s) 762, 777, 900, 922
AluBI AGCT 8 cut(s) 121, 199, 316, 409, 430, 757, 859, 1181
AluI AGCT 8 cut(s) 121, 199, 316, 409, 430, 757, 859, 1181
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 246, 884
AlwI GGATC 3 cut(s) 450, 1118, 1131
Ama87I CYCGRG 3 cut(s) 306, 453, 1130
AoxI GGCC 8 cut(s) 20, 26, 309, 680, 846, 1001, 1261, 1270
ApeKI GCWGC 3 cut(s) 634, 1178, 1181
ApoI RAATTY 2 cut(s) 390, 1327
ArsI GACNNNNNNTTYG 2 cut(s) 159, 191
AseI ATTAAT 1 cut(s) 1277
AsiGI ACCGGT 1 cut(s) 646
Asp700I GAANNNNTTC 2 cut(s) 393, 756
AspLEI GCGC 2 cut(s) 296, 774
AspS9I GGNCC 5 cut(s) 27, 175, 309, 781, 1270
AsuC2I CCSGG 2 cut(s) 307, 308
AsuHPI GGTGA 3 cut(s) 332, 392, 483
AsuII TTCGAA 1 cut(s) 78
AsuNHI GCTAGC 1 cut(s) 199
AvaI CYCGRG 3 cut(s) 306, 453, 1130
AvaII GGWCC 2 cut(s) 175, 781
BamHI GGATCC 1 cut(s) 1123
BanII GRGCYC 2 cut(s) 307, 318
BarI GAAGNNNNNNTAC 2 cut(s) 1000, 1032
BauI CACGAG 1 cut(s) 709
BbsI GAAGAC 3 cut(s) 87, 667, 933
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 3 cut(s) 646, 1168, 1190
BccI CCATC 3 cut(s) 600, 1207, 1284
BceAI ACGGC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 700, 734
BciT130I CCWGG 4 cut(s) 764, 779, 902, 924
BclI TGATCA 1 cut(s) 1056
BcnI CCSGG 2 cut(s) 307, 308
BcoDI GTCTC 2 cut(s) 246, 884
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 1182
BglI GCCNNNNNGGC 1 cut(s) 1269
BisI GCNGC 4 cut(s) 93, 635, 1179, 1182
BlsI GCNGC 4 cut(s) 94, 636, 1180, 1183
Bme1390I CCNGG 6 cut(s) 307, 308, 764, 779, 902, 924
Bme18I GGWCC 2 cut(s) 175, 781
BmeT110I CYCGRG 3 cut(s) 306, 453, 1130
BmgT120I GGNCC 5 cut(s) 27, 175, 309, 781, 1270
BmiI GGNNCC 7 cut(s) 29, 50, 304, 1066, 1125, 1271, 1295
BmrFI CCNGG 6 cut(s) 307, 308, 764, 779, 902, 924
BmsI GCATC 2 cut(s) 708, 1293
BmtI GCTAGC 1 cut(s) 203
BpiI GAAGAC 3 cut(s) 87, 667, 933
BpmI CTGGAG 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 431, 1086
BpuMI CCSGG 2 cut(s) 307, 308
BsaHI GRCGYC 1 cut(s) 1087
BsaJI CCNNGG 5 cut(s) 23, 306, 683, 1249, 1264
BsaWI WCCGGW 2 cut(s) 567, 646
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 1136
BseBI CCWGG 4 cut(s) 764, 779, 902, 924
BseDI CCNNGG 5 cut(s) 23, 306, 683, 1249, 1264
BseGI GGATG 4 cut(s) 36, 358, 564, 723
BseMII CTCAG 4 cut(s) 131, 236, 519, 565
BseNI ACTGG 1 cut(s) 1136
BseRI GAGGAG 8 cut(s) 113, 136, 139, 210, 315, 398, 630, 977
BseXI GCAGC 3 cut(s) 646, 1168, 1190
BsgI GTGCAG 2 cut(s) 689, 1005
Bsh1236I CGCG 2 cut(s) 294, 1251
BshFI GGCC 8 cut(s) 22, 28, 311, 682, 848, 1003, 1263, 1272
BshTI ACCGGT 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 3 cut(s) 306, 453, 1130
BsiSI CCGG 3 cut(s) 307, 568, 647
BsmAI GTCTC 2 cut(s) 246, 884
BsnI GGCC 8 cut(s) 22, 28, 311, 682, 848, 1003, 1263, 1272
BsoBI CYCGRG 3 cut(s) 306, 453, 1130
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 307, 318
Bsp143I GATC 3 cut(s) 442, 1056, 1123
BspANI GGCC 8 cut(s) 22, 28, 311, 682, 848, 1003, 1263, 1272
BspCNI CTCAG 4 cut(s) 130, 235, 520, 564
BspFNI CGCG 2 cut(s) 294, 1251
BspLI GGNNCC 7 cut(s) 29, 50, 304, 1066, 1125, 1271, 1295
BspMAI CTGCAG 1 cut(s) 1186
BspOI GCTAGC 1 cut(s) 203
BspPI GGATC 3 cut(s) 450, 1118, 1131
BspT104I TTCGAA 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 1 cut(s) 1136
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 5 cut(s) 23, 306, 683, 1249, 1264
BssMI GATC 3 cut(s) 442, 1056, 1123
BssNI GRCGYC 1 cut(s) 1087
BssSI CACGAG 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 1 cut(s) 709
Bst2UI CCWGG 4 cut(s) 764, 779, 902, 924
Bst4CI ACNGT 1 cut(s) 998
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 1087
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 201, 1095, 1186
BstDEI CTNAG 5 cut(s) 117, 222, 528, 551, 1159
BstDSI CCRYGG 1 cut(s) 1249
BstF5I GGATG 4 cut(s) 36, 358, 564, 723
BstFNI CGCG 2 cut(s) 294, 1251
BstHHI GCGC 2 cut(s) 296, 774
BstKTI GATC 3 cut(s) 445, 1059, 1126
BstMAI GTCTC 2 cut(s) 246, 884
BstMBI GATC 3 cut(s) 442, 1056, 1123
BstMWI GCNNNNNNNGC 6 cut(s) 302, 530, 622, 631, 1033, 1269
BstNI CCWGG 4 cut(s) 764, 779, 902, 924
BstNSI RCATGY 1 cut(s) 1097
BstSCI CCNGG 6 cut(s) 305, 306, 762, 777, 900, 922
BstSFI CTRYAG 1 cut(s) 1182
BstUI CGCG 2 cut(s) 294, 1251
BstV1I GCAGC 3 cut(s) 646, 1168, 1190
BstV2I GAAGAC 3 cut(s) 87, 667, 933
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuRI GGCC 8 cut(s) 22, 28, 311, 682, 848, 1003, 1263, 1272
BtgI CCRYGG 1 cut(s) 1249
BtsCI GGATG 4 cut(s) 36, 358, 564, 723
BtsI GCAGTG 2 cut(s) 893, 1022
BtsIMutI CAGTG 2 cut(s) 893, 1022
Cac8I GCNNGC 3 cut(s) 201, 1095, 1186
CfoI GCGC 2 cut(s) 296, 774
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 5 cut(s) 27, 175, 309, 781, 1270
Cfr42I CCGCGG 1 cut(s) 1252
Cfr9I CCCGGG 1 cut(s) 306
CseI GACGC 1 cut(s) 1076
Csp6I GTAC 4 cut(s) 503, 880, 1008, 1193
CspAI ACCGGT 1 cut(s) 646
CviAII CATG 6 cut(s) 539, 841, 887, 937, 1069, 1094
CviQI GTAC 4 cut(s) 503, 880, 1008, 1193
DdeI CTNAG 5 cut(s) 117, 222, 528, 551, 1159
DpnI GATC 3 cut(s) 444, 1058, 1125
DpnII GATC 3 cut(s) 442, 1056, 1123
EaeI YGGCCR 1 cut(s) 1261
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 5 cut(s) 116, 124, 142, 148, 1023
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 683
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 2 cut(s) 307, 318
Eco47I GGWCC 2 cut(s) 175, 781
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 661
Eco88I CYCGRG 3 cut(s) 306, 453, 1130
EcoICRI GAGCTC 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 1327
EcoRII CCWGG 4 cut(s) 762, 777, 900, 922
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 307, 318
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 6 cut(s) 542, 844, 890, 940, 1072, 1097
FalI AAGNNNNNCTT 6 cut(s) 605, 637, 843, 875, 1095, 1127
FatI CATG 6 cut(s) 538, 840, 886, 936, 1068, 1093
FauI CCCGC 2 cut(s) 116, 196
FbaI TGATCA 1 cut(s) 1056
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 4 cut(s) 93, 635, 1179, 1182
FokI GGATG 4 cut(s) 23, 365, 551, 730
FriOI GRGCYC 2 cut(s) 307, 318
Fsp4HI GCNGC 4 cut(s) 93, 635, 1179, 1182
FspBI CTAG 1 cut(s) 200
GlaI GCGC 2 cut(s) 295, 773
GluI GCNGC 4 cut(s) 93, 635, 1179, 1182
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 8 cut(s) 22, 28, 311, 682, 848, 1003, 1263, 1272
HapII CCGG 3 cut(s) 307, 568, 647
HgaI GACGC 1 cut(s) 1076
HhaI GCGC 2 cut(s) 296, 774
Hin1I GRCGYC 1 cut(s) 1087
Hin1II CATG 6 cut(s) 542, 844, 890, 940, 1072, 1097
Hin6I GCGC 2 cut(s) 294, 772
HinP1I GCGC 2 cut(s) 294, 772
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 2 cut(s) 407, 755
HinfI GANTC 5 cut(s) 143, 217, 415, 977, 1205
HpaII CCGG 3 cut(s) 307, 568, 647
HphI GGTGA 3 cut(s) 332, 392, 483
Hpy166II GTNNAC 3 cut(s) 142, 178, 1010
Hpy188I TCNGA 3 cut(s) 554, 982, 1204
Hpy188III TCNNGA 4 cut(s) 214, 455, 814, 1166
Hpy8I GTNNAC 3 cut(s) 142, 178, 1010
Hpy99I CGWCG 4 cut(s) 59, 143, 630, 1092
HpyAV CCTTC 4 cut(s) 708, 720, 929, 1026
HpyCH4III ACNGT 1 cut(s) 998
HpyCH4IV ACGT 1 cut(s) 628
HpyCH4V TGCA 8 cut(s) 634, 706, 721, 742, 1022, 1097, 1184, 1306
HpyF10VI GCNNNNNNNGC 6 cut(s) 302, 530, 622, 631, 1033, 1269
HpyF3I CTNAG 5 cut(s) 117, 222, 528, 551, 1159
HpySE526I ACGT 1 cut(s) 628
Hsp92I GRCGYC 1 cut(s) 1087
Hsp92II CATG 6 cut(s) 542, 844, 890, 940, 1072, 1097
HspAI GCGC 2 cut(s) 294, 772
Ksp22I TGATCA 1 cut(s) 1056
KspI CCGCGG 1 cut(s) 1252
Kzo9I GATC 3 cut(s) 442, 1056, 1123
LmnI GCTCC 5 cut(s) 54, 126, 302, 856, 903
Lsp1109I GCAGC 3 cut(s) 646, 1168, 1190
LweI GCATC 2 cut(s) 708, 1293
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 1 cut(s) 1225
MalI GATC 3 cut(s) 444, 1058, 1125
MboI GATC 3 cut(s) 442, 1056, 1123
MboII GAAGA 9 cut(s) 92, 380, 386, 667, 833, 917, 933, 1200, 1266
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 2 cut(s) 307, 318
MluCI AATT 6 cut(s) 112, 280, 390, 1257, 1327, 1369
MlyI GAGTC 3 cut(s) 137, 211, 424
MroXI GAANNNNTTC 2 cut(s) 393, 756
MseI TTAA 4 cut(s) 651, 809, 1242, 1277
MspA1I CMGCKG 2 cut(s) 1181, 1251
MspI CCGG 3 cut(s) 307, 568, 647
MspR9I CCNGG 6 cut(s) 307, 308, 764, 779, 902, 924
MvaI CCWGG 4 cut(s) 764, 779, 902, 924
MvnI CGCG 2 cut(s) 294, 1251
MwoI GCNNNNNNNGC 6 cut(s) 302, 530, 622, 631, 1033, 1269
NciI CCSGG 2 cut(s) 307, 308
NdeII GATC 3 cut(s) 442, 1056, 1123
NheI GCTAGC 1 cut(s) 199
NlaIII CATG 6 cut(s) 542, 844, 890, 940, 1072, 1097
NlaIV GGNNCC 7 cut(s) 29, 50, 304, 1066, 1125, 1271, 1295
NmeAIII GCCGAG 3 cut(s) 4, 337, 1289
NspI RCATGY 1 cut(s) 1097
NspV TTCGAA 1 cut(s) 78
PaeI GCATGC 1 cut(s) 1097
PaeR7I CTCGAG 1 cut(s) 1130
PceI AGGCCT 1 cut(s) 22
PdmI GAANNNNTTC 2 cut(s) 393, 756
PfeI GAWTC 2 cut(s) 977, 1205
PfoI TCCNGGA 1 cut(s) 900
PinAI ACCGGT 1 cut(s) 646
PkrI GCNGC 4 cut(s) 94, 636, 1180, 1183
PleI GAGTC 3 cut(s) 137, 211, 423
PpsI GAGTC 3 cut(s) 137, 211, 423
PshBI ATTAAT 1 cut(s) 1277
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 4 cut(s) 762, 777, 900, 922
PspGI CCWGG 4 cut(s) 762, 777, 900, 922
PspN4I GGNNCC 7 cut(s) 29, 50, 304, 1066, 1125, 1271, 1295
PspPI GGNCC 5 cut(s) 27, 175, 309, 781, 1270
PspXI VCTCGAGB 1 cut(s) 1130
PstI CTGCAG 1 cut(s) 1186
PsuI RGATCY 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 4 cut(s) 504, 881, 1009, 1194
RsaNI GTAC 4 cut(s) 503, 880, 1008, 1193
SacI GAGCTC 1 cut(s) 318
SacII CCGCGG 1 cut(s) 1252
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 651, 809, 1242, 1277
SatI GCNGC 4 cut(s) 93, 635, 1179, 1182
Sau3AI GATC 3 cut(s) 442, 1056, 1123
Sau96I GGNCC 5 cut(s) 27, 175, 309, 781, 1270
SchI GAGTC 3 cut(s) 137, 211, 424
ScrFI CCNGG 6 cut(s) 307, 308, 764, 779, 902, 924
SduI GDGCHC 2 cut(s) 307, 318
SfaNI GCATC 2 cut(s) 708, 1293
SfcI CTRYAG 1 cut(s) 1182
SfiI GGCCNNNNNGGCC 1 cut(s) 1269
Sfr274I CTCGAG 1 cut(s) 1130
Sfr303I CCGCGG 1 cut(s) 1252
SfuI TTCGAA 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 1252
SinI GGWCC 2 cut(s) 175, 781
SlaI CTCGAG 1 cut(s) 1130
SmaI CCCGGG 1 cut(s) 308
SmlI CTYRAG 3 cut(s) 410, 1101, 1130
SmoI CTYRAG 3 cut(s) 410, 1101, 1130
SphI GCATGC 1 cut(s) 1097
SrfI GCCCGGGC 1 cut(s) 308
Sse9I AATT 6 cut(s) 112, 280, 390, 1257, 1327, 1369
SseBI AGGCCT 1 cut(s) 22
SspI AATATT 1 cut(s) 1281
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 318
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 6 cut(s) 305, 306, 762, 777, 900, 922
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 1 cut(s) 998
TaiI ACGT 1 cut(s) 631
TaqI TCGA 7 cut(s) 33, 78, 141, 171, 1131, 1317, 1354
TasI AATT 6 cut(s) 112, 280, 390, 1257, 1327, 1369
TatI WGTACW 2 cut(s) 502, 879
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 2 cut(s) 977, 1205
Tru1I TTAA 4 cut(s) 651, 809, 1242, 1277
Tru9I TTAA 4 cut(s) 651, 809, 1242, 1277
TscAI CASTG 2 cut(s) 900, 1029
TseI GCWGC 3 cut(s) 634, 1178, 1181
TspDTI ATGAA 5 cut(s) 102, 890, 1085, 1103, 1320
TspMI CCCGGG 1 cut(s) 306
TspRI CASTG 2 cut(s) 900, 1029
VpaK11BI GGWCC 2 cut(s) 175, 781
VspI ATTAAT 1 cut(s) 1277
XapI RAATTY 2 cut(s) 390, 1327
XceI RCATGY 1 cut(s) 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 796
XhoI CTCGAG 1 cut(s) 1130
XmaI CCCGGG 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 2 cut(s) 393, 756
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.