FvH4_6g36660

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005820
Physical Location & Seq
Forward (+)
28852830 .. 28859749
6920 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36660.t1

Sequence Viewer

Length: 2406 bp
ATGCTGATGGGTGATGATTTGGAGGATGGAAATTTGGGGCCTTACCAAGAGAGGCTGAGGACATTTCCTAACATGCGTGGTAAATCTTACAACCCATTGATTTTTCGAATTTTGATGGGGATAAATGTTCGTGTCCTTGTCGTGATTTTGCTTCTGGCACTTGGAGTGGTCTTTTACATTGGAGCACGCACTTCTCCTATTATTGTGTTCGTCTTCTCAGTTTGCATTATCAGCTTCCTTCTGGCAATGCACCTTGCCAAATGGGTTCTCGCAAAAGATGAGGGACCTCCGGAGATGGGTCAGATATCAGATGCAATACGTGATGGGGCTGAAGGTTTCTTCAGGACCCAGTATGGGACCATCTCTAAGATGGCAATTTTACTGGCTGCAGTTATCCTCTGCATATACTTATTTCGCACTACAACTCCTCAGCAAGAGTCATCTGGTCTTGGAAGGGTAGCCACCGCATTTATTACTGTAGCTGCATTTCTTTTGGGAGCTTTCTGCTCAGGTGTTGCAGGGTATGTTGGGATGTGGGTATCAGTTCGTGCCAATGTTCGAGTCTCTAGTGCCGCAAGACGATCTGCACGGGAGGCACTGCAGATTGCTGTTCGTGCTGGTGGATTTTCTGCTATGATTGTTGTTGGTATGGCTGTAATCGGTATAGCCATCCTGTATGCCACATTTTATGTTTGGTTGGGAGTGGATACACCAGGTTCAATGAAGGTTACTGACTTGCCTCTTCTTCTTGTTGGATACGGCTTCGGAGCTTCTTTTGTTGCCTTATTTGCTCAGCTGGGTGGCGGAATATACACAAAAGCAGCTGATGTTGGAGCAGATCTTGTTGGAAAAGTAGAGCAGGGAATTCCTGAAGATGATCCTAGGAATCCTGCTGTGATAGCAGATCTGGTTGGAGATAATGTTGGGGATTGCGCAGCTAGAGGTGCTGATCTTTTTGAAAGTATTGCTGCTGAAATAATCAGTGCTATGATACTTGGAGGAACCATGGCTCAACGTTGTAAAATTGAAGATCCTTCTGGTTTCATCTTGTTTCCTCTGGTTGTTCACTCCTTTGATCTGGTGATTTCATCGGTTGGAATACTCTCAATCAGGGGTACACGAGATTCTGGTGCGAAGGGTTCTGAGGATCCAATGGCAATTCTTGAGAGAGGATACTCAATCACAGTAATTTTAGCTGTTCTGACATTTGGTGCGTCAACCCGATGGTTGTTATACACCGAACAAGCACCGTCTGCGTGGTTTCACTTTGCCTTGTGTGGATTAGTTGGCATCATCACTGCATATATATTTGTGTGGATAACCAAGTACTATACAGACTACAAATATGAGCCTGTACGCACATTAGCTCTTGCAAGCTCCACCGGTCATGGGACTAATATCATTGCTGGAGTCAGTTTGGGTCTGGAATCAACAGCTCTTCCTGTTCTTGTCATTAGCGTAGCTATTGTCTCAGCTTACTGGTTGGGCCAGACCTCGGGACTCGTGGACGAAAATGGTGTTCCTACTGGCGGGCTATTTGGTACAGCTGTAGCAACAATGGGCATGCTCAGTACTGCTGCTTATGTTCTCACGATGGATATGTTTGGTCCAATAGCTGATAATGCTGGTGGAATTGTAGAGATGAGTCAGCAGCCAGAAAGTGTTCGCGAGATCACAGATGTTCTTGATGCAGTAGGCAACACTACAAAAGCTACCACTAAAGGGTTTGCCATTGGATCTGCTGCACTTGCATCTTTTCTTCTATTTAGTGCTTATATGGATGAGGTAGCTACATTTGCTCGTGAACCTTTCAAGCAGGTTGATATTGCCATTCCAGAAGTTTTTGTCGGTGGATTGTTGGGCTCTATGCTTATTTTCTTATTTAGTGCTTGGGCCTGTTCAGCAGTTGGCCGAACTGCTCAAGAGGTTGTCAGGGAAGTAAGAAGGCAATTTATTGAGCGGCCTGGTATAATGGAATACAAAGAGAAGCCAGATTATGCACGTTGTGTTGCTATAGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGTCTTGGCTATAGTTTCACCTATAGCAGTTGGTATTGTGTTCCGGATATTTGGATACTATACTGGACAGCCTCTTCTTGGTGCTAAAGTTGTGGCTTCCATGCTGATGTTTGCAACAGTGTCGGGTATTCTCATGGCTCTTTTCCTGAACACGTCAGGTGGTGCCTGGGATAATGCAAAGAAGTACATCGAGACGGGGGTTCTCGGAGGCAAAGGAAGTGATTGTCATAAGGCTGCAGTCACAGGAGACACAGTGGGAGACCCATTTAAAGACACAGCTGGACCTTCACTTCACGTCCTCATTAAAATGCTTGCAACAATTACGCTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

802

Amino Acids

85.04

Weight (kDa)

5.62

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 76 - 796 6e-256 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 934
Acc36I ACCTGC 1 cut(s) 1807
AccB1I GGYRCC 1 cut(s) 2216
AccBSI CCGCTC 1 cut(s) 1960
AccII CGCG 1 cut(s) 1668
AccIII TCCGGA 2 cut(s) 289, 2097
AciI CCGC 5 cut(s) 465, 573, 804, 1530, 1960
AclI AACGTT 1 cut(s) 1015
AclWI GGATC 5 cut(s) 872, 1025, 1142, 1155, 1744
AcoI YGGCCR 1 cut(s) 1909
AcsI RAATTY 3 cut(s) 31, 108, 864
AcuI CTGAAG 3 cut(s) 325, 351, 891
AdeI CACNNNGTG 1 cut(s) 2006
AfaI GTAC 6 cut(s) 1117, 1328, 1356, 1543, 1573, 2240
AfiI CCNNNNNNNGG 7 cut(s) 296, 354, 1389, 1495, 1529, 1722, 2132
AflIII ACRYGT 1 cut(s) 2205
AgeI ACCGGT 1 cut(s) 1382
AgsI TTSAA 4 cut(s) 720, 959, 1028, 1813
AjiI CACGTC 2 cut(s) 2208, 2350
AjnI CCWGG 4 cut(s) 712, 1963, 2050, 2219
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 5 cut(s) 568, 1474, 2240, 2295, 2307
AlwI GGATC 5 cut(s) 872, 1025, 1142, 1155, 1744
Ama87I CYCGRG 1 cut(s) 1495
Aor13HI TCCGGA 2 cut(s) 289, 2097
AoxI GGCC 5 cut(s) 38, 1486, 1893, 1909, 1961
ApeKI GCWGC 9 cut(s) 386, 482, 821, 935, 968, 1577, 1651, 1742, 2288
ApoI RAATTY 3 cut(s) 31, 108, 864
AsiGI ACCGGT 1 cut(s) 1382
Asp700I GAANNNNTTC 1 cut(s) 1662
AspA2I CCTAGG 1 cut(s) 881
AspLEI GCGC 1 cut(s) 935
AspS9I GGNCC 8 cut(s) 38, 284, 345, 357, 1486, 1607, 1893, 2336
AsuHPI GGTGA 3 cut(s) 23, 1093, 2064
AsuII TTCGAA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 1495
AvaII GGWCC 5 cut(s) 284, 345, 357, 1607, 2336
AvrII CCTAGG 1 cut(s) 881
BaeI ACNNNNGTAYC 2 cut(s) 1533, 1566
BamHI GGATCC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 2216
BanII GRGCYC 1 cut(s) 1865
BauI CACGAG 3 cut(s) 1119, 1502, 1800
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 2 cut(s) 56, 429
BbvI GCAGC 9 cut(s) 373, 469, 833, 947, 955, 1564, 1663, 1729, 2275
BccI CCATC 9 cut(s) 20, 109, 289, 317, 364, 368, 677, 1218, 1588
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2157, 2191
BciT130I CCWGG 4 cut(s) 714, 1965, 2052, 2221
BciVI GTATCC 4 cut(s) 700, 749, 1166, 2102
BcoDI GTCTC 5 cut(s) 568, 1474, 2240, 2295, 2307
BfaI CTAG 3 cut(s) 567, 882, 939
BfmI CTRYAG 8 cut(s) 387, 477, 599, 1548, 2013, 2064, 2076, 2289
BfuAI ACCTGC 1 cut(s) 1807
BfuI GTATCC 4 cut(s) 700, 749, 1166, 2102
BglII AGATCT 2 cut(s) 838, 904
BlnI CCTAGG 1 cut(s) 881
BlpI GCTNAGC 1 cut(s) 792
BmcAI AGTACT 2 cut(s) 1328, 1573
Bme1390I CCNGG 4 cut(s) 714, 1965, 2052, 2221
Bme18I GGWCC 5 cut(s) 284, 345, 357, 1607, 2336
BmeT110I CYCGRG 1 cut(s) 1495
BmgBI CACGTC 2 cut(s) 2208, 2350
BmgT120I GGNCC 8 cut(s) 38, 284, 345, 357, 1486, 1607, 1893, 2336
BmiI GGNNCC 8 cut(s) 39, 285, 347, 358, 1003, 1149, 2218, 2391
BmrFI CCNGG 4 cut(s) 714, 1965, 2052, 2221
BmrI ACTGGG 1 cut(s) 343
BmsI GCATC 6 cut(s) 301, 1299, 1678, 1760, 2030, 2036
BmuI ACTGGG 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 205
BpmI CTGGAG 1 cut(s) 1428
Bpu10I CCTNAGC 3 cut(s) 56, 429, 508
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 2 cut(s) 1184, 1905
BsaAI YACGTR 1 cut(s) 320
BsaI GGTCTC 1 cut(s) 2307
BsaJI CCNNGG 4 cut(s) 881, 1005, 1494, 2220
BsaWI WCCGGW 3 cut(s) 289, 1382, 2097
BsaXI ACNNNNNCTCC 6 cut(s) 409, 439, 906, 936, 2292, 2322
Bsc4I CCNNNNNNNGG 7 cut(s) 296, 354, 1389, 1495, 1529, 1722, 2132
Bse118I RCCGGY 1 cut(s) 1382
Bse1I ACTGG 5 cut(s) 349, 387, 1484, 1531, 2122
Bse3DI GCAATG 2 cut(s) 252, 1401
BseAI TCCGGA 2 cut(s) 289, 2097
BseBI CCWGG 4 cut(s) 714, 1965, 2052, 2221
BseDI CCNNGG 4 cut(s) 881, 1005, 1494, 2220
BseGI GGATG 4 cut(s) 31, 537, 669, 1786
BseLI CCNNNNNNNGG 7 cut(s) 296, 354, 1389, 1495, 1529, 1722, 2132
BseMI GCAATG 2 cut(s) 252, 1401
BseMII CTCAG 8 cut(s) 47, 231, 443, 522, 806, 1134, 1485, 1582
BseNI ACTGG 5 cut(s) 349, 387, 1484, 1531, 2122
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 9 cut(s) 373, 469, 833, 947, 955, 1564, 1663, 1729, 2275
BseYI CCCAGC 1 cut(s) 796
BsgI GTGCAG 2 cut(s) 570, 1728
Bsh1236I CGCG 1 cut(s) 1668
BshFI GGCC 5 cut(s) 40, 1488, 1895, 1911, 1963
BshNI GGYRCC 1 cut(s) 2216
BshTI ACCGGT 1 cut(s) 1382
BsiHKAI GWGCWC 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 1495
BsiSI CCGG 3 cut(s) 290, 1383, 2098
BslFI GGGAC 4 cut(s) 297, 370, 1405, 1512
BslI CCNNNNNNNGG 7 cut(s) 296, 354, 1389, 1495, 1529, 1722, 2132
BsmAI GTCTC 5 cut(s) 568, 1474, 2240, 2295, 2307
BsmBI CGTCTC 1 cut(s) 2240
BsmFI GGGAC 4 cut(s) 297, 370, 1405, 1512
BsnI GGCC 5 cut(s) 40, 1488, 1895, 1911, 1963
Bso31I GGTCTC 1 cut(s) 2307
BsoBI CYCGRG 1 cut(s) 1495
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 187, 1865
Bsp13I TCCGGA 2 cut(s) 289, 2097
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp19I CCATGG 1 cut(s) 1005
Bsp68I TCGCGA 1 cut(s) 1668
BspACI CCGC 5 cut(s) 465, 573, 804, 1530, 1960
BspANI GGCC 5 cut(s) 40, 1488, 1895, 1911, 1963
BspCNI CTCAG 8 cut(s) 48, 230, 442, 521, 805, 1135, 1484, 1581
BspEI TCCGGA 2 cut(s) 289, 2097
BspFNI CGCG 1 cut(s) 1668
BspLI GGNNCC 8 cut(s) 39, 285, 347, 358, 1003, 1149, 2218, 2391
BspMAI CTGCAG 3 cut(s) 391, 603, 2293
BspMI ACCTGC 1 cut(s) 1807
BspPI GGATC 5 cut(s) 872, 1025, 1142, 1155, 1744
BspQI GCTCTTC 1 cut(s) 1443
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 2216
BspTNI GGTCTC 1 cut(s) 2307
BsrBI CCGCTC 1 cut(s) 1960
BsrDI GCAATG 2 cut(s) 252, 1401
BsrFI RCCGGY 1 cut(s) 1382
BsrI ACTGG 5 cut(s) 349, 387, 1484, 1531, 2122
BssAI RCCGGY 1 cut(s) 1382
BssECI CCNNGG 4 cut(s) 881, 1005, 1494, 2220
BssSI CACGAG 3 cut(s) 1119, 1502, 1800
BssT1I CCWWGG 2 cut(s) 881, 1005
Bst2BI CACGAG 3 cut(s) 1119, 1502, 1800
Bst2UI CCWGG 4 cut(s) 714, 1965, 2052, 2221
Bst4CI ACNGT 5 cut(s) 478, 1186, 1251, 2173, 2308
Bst6I CTCTTC 3 cut(s) 747, 1443, 2133
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstBAI YACGTR 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 5 cut(s) 187, 1375, 1532, 1565, 2367
BstDEI CTNAG 9 cut(s) 56, 217, 366, 429, 508, 792, 1143, 1471, 1568
BstDSI CCRYGG 1 cut(s) 1005
BstF5I GGATG 4 cut(s) 31, 537, 669, 1786
BstFNI CGCG 1 cut(s) 1668
BstHHI GCGC 1 cut(s) 935
BstMAI GTCTC 5 cut(s) 568, 1474, 2240, 2295, 2307
BstNI CCWGG 4 cut(s) 714, 1965, 2052, 2221
BstNSI RCATGY 2 cut(s) 76, 1567
BstSCI CCNGG 4 cut(s) 712, 1963, 2050, 2219
BstSFI CTRYAG 8 cut(s) 387, 477, 599, 1548, 2013, 2064, 2076, 2289
BstUI CGCG 1 cut(s) 1668
BstV1I GCAGC 9 cut(s) 373, 469, 833, 947, 955, 1564, 1663, 1729, 2275
BstV2I GAAGAC 1 cut(s) 205
BstX2I RGATCY 5 cut(s) 838, 904, 1030, 1147, 1736
BstYI RGATCY 5 cut(s) 838, 904, 1030, 1147, 1736
BsuI GTATCC 4 cut(s) 700, 749, 1166, 2102
BsuRI GGCC 5 cut(s) 40, 1488, 1895, 1911, 1963
BtgI CCRYGG 1 cut(s) 1005
BtrI CACGTC 2 cut(s) 2208, 2350
BtsCI GGATG 4 cut(s) 31, 537, 669, 1786
BtsI GCAGTG 2 cut(s) 596, 1296
BtsIMutI CAGTG 5 cut(s) 596, 988, 1296, 2178, 2313
BtuMI TCGCGA 1 cut(s) 1668
BveI ACCTGC 1 cut(s) 1807
Cac8I GCNNGC 5 cut(s) 187, 1375, 1532, 1565, 2367
CfoI GCGC 1 cut(s) 935
Cfr10I RCCGGY 1 cut(s) 1382
Cfr13I GGNCC 8 cut(s) 38, 284, 345, 357, 1486, 1607, 1893, 2336
CseI GACGC 1 cut(s) 1203
CsiI ACCWGGT 2 cut(s) 712, 2050
Csp6I GTAC 6 cut(s) 1116, 1327, 1355, 1542, 1572, 2239
CspAI ACCGGT 1 cut(s) 1382
CviAII CATG 7 cut(s) 73, 1006, 1388, 1564, 2155, 2188, 2386
CviQI GTAC 6 cut(s) 1116, 1327, 1355, 1542, 1572, 2239
DdeI CTNAG 9 cut(s) 56, 217, 366, 429, 508, 792, 1143, 1471, 1568
DraI TTTAAA 1 cut(s) 2323
DraIII CACNNNGTG 1 cut(s) 2006
EaeI YGGCCR 1 cut(s) 1909
Eam1104I CTCTTC 3 cut(s) 747, 1443, 2133
EarI CTCTTC 3 cut(s) 747, 1443, 2133
EciI GGCGGA 1 cut(s) 819
Eco130I CCWWGG 2 cut(s) 881, 1005
Eco24I GRGCYC 1 cut(s) 1865
Eco31I GGTCTC 1 cut(s) 2307
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 5 cut(s) 284, 345, 357, 1607, 2336
Eco57I CTGAAG 3 cut(s) 325, 351, 891
Eco88I CYCGRG 1 cut(s) 1495
EcoO109I RGGNCCY 3 cut(s) 38, 284, 345
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 4 cut(s) 712, 1963, 2050, 2219
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 2 cut(s) 881, 1005
EcoT38I GRGCYC 1 cut(s) 1865
ErhI CCWWGG 2 cut(s) 881, 1005
Esp3I CGTCTC 1 cut(s) 2240
FaeI CATG 7 cut(s) 76, 1009, 1391, 1567, 2158, 2191, 2389
FaqI GGGAC 4 cut(s) 297, 370, 1405, 1512
FatI CATG 7 cut(s) 72, 1005, 1387, 1563, 2154, 2187, 2385
FauI CCCGC 1 cut(s) 1523
FokI GGATG 4 cut(s) 38, 544, 656, 1793
FriOI GRGCYC 1 cut(s) 1865
FspBI CTAG 3 cut(s) 567, 882, 939
FspI TGCGCA 1 cut(s) 934
GlaI GCGC 1 cut(s) 934
GsaI CCCAGC 1 cut(s) 800
GsuI CTGGAG 1 cut(s) 1428
HaeIII GGCC 5 cut(s) 40, 1488, 1895, 1911, 1963
HapII CCGG 3 cut(s) 290, 1383, 2098
HgaI GACGC 1 cut(s) 1203
HhaI GCGC 1 cut(s) 935
Hin1II CATG 7 cut(s) 76, 1009, 1391, 1567, 2158, 2191, 2389
Hin6I GCGC 1 cut(s) 933
HinP1I GCGC 1 cut(s) 933
HincII GTYRAC 1 cut(s) 1218
HindII GTYRAC 1 cut(s) 1218
HinfI GANTC 8 cut(s) 437, 561, 886, 1124, 1410, 1427, 1500, 1645
HpaII CCGG 3 cut(s) 290, 1383, 2098
HphI GGTGA 3 cut(s) 23, 1093, 2064
Hpy166II GTNNAC 5 cut(s) 1066, 1118, 1218, 1507, 1805
Hpy188I TCNGA 7 cut(s) 303, 310, 767, 1144, 1203, 2261, 2405
Hpy8I GTNNAC 5 cut(s) 1066, 1118, 1218, 1507, 1805
HpyAV CCTTC 8 cut(s) 248, 326, 447, 718, 1044, 1129, 1938, 2349
HpyCH4III ACNGT 5 cut(s) 478, 1186, 1251, 2173, 2308
HpyCH4IV ACGT 5 cut(s) 319, 1015, 2002, 2207, 2349
HpyF3I CTNAG 9 cut(s) 56, 217, 366, 429, 508, 792, 1143, 1471, 1568
HpySE526I ACGT 5 cut(s) 319, 1015, 2002, 2207, 2349
Hsp92II CATG 7 cut(s) 76, 1009, 1391, 1567, 2158, 2191, 2389
HspAI GCGC 1 cut(s) 933
Kpn2I TCCGGA 2 cut(s) 289, 2097
LguI GCTCTTC 1 cut(s) 1443
LmnI GCTCC 6 cut(s) 182, 497, 767, 833, 1382, 2395
Lsp1109I GCAGC 9 cut(s) 373, 469, 833, 947, 955, 1564, 1663, 1729, 2275
LweI GCATC 6 cut(s) 301, 1299, 1678, 1760, 2030, 2036
MabI ACCWGGT 2 cut(s) 712, 2050
MaeI CTAG 3 cut(s) 567, 882, 939
MaeII ACGT 5 cut(s) 319, 1015, 2002, 2207, 2349
MaeIII GTNAC 2 cut(s) 727, 2293
MbiI CCGCTC 1 cut(s) 1960
MboII GAAGA 9 cut(s) 205, 331, 734, 737, 884, 1040, 1430, 1751, 2120
MflI RGATCY 5 cut(s) 838, 904, 1030, 1147, 1736
MhlI GDGCHC 2 cut(s) 187, 1865
MlyI GAGTC 5 cut(s) 446, 570, 1419, 1494, 1654
MmeI TCCRAC 5 cut(s) 733, 811, 826, 892, 1075
MroI TCCGGA 2 cut(s) 289, 2097
MroXI GAANNNNTTC 1 cut(s) 1662
MseI TTAA 2 cut(s) 2322, 2358
MslI CAYNNNNRTG 2 cut(s) 2159, 2360
MspA1I CMGCKG 4 cut(s) 796, 824, 1547, 2333
MspI CCGG 3 cut(s) 290, 1383, 2098
MspR9I CCNGG 4 cut(s) 714, 1965, 2052, 2221
MvaI CCWGG 4 cut(s) 714, 1965, 2052, 2221
MvnI CGCG 1 cut(s) 1668
NcoI CCATGG 1 cut(s) 1005
NlaIII CATG 7 cut(s) 76, 1009, 1391, 1567, 2158, 2191, 2389
NlaIV GGNNCC 8 cut(s) 39, 285, 347, 358, 1003, 1149, 2218, 2391
NmuCI GTSAC 1 cut(s) 2293
NruI TCGCGA 1 cut(s) 1668
NsbI TGCGCA 1 cut(s) 934
NspI RCATGY 2 cut(s) 76, 1567
NspV TTCGAA 1 cut(s) 106
PaeI GCATGC 1 cut(s) 1567
PciSI GCTCTTC 1 cut(s) 1443
PdmI GAANNNNTTC 1 cut(s) 1662
PfeI GAWTC 3 cut(s) 886, 1124, 1427
PinAI ACCGGT 1 cut(s) 1382
PleI GAGTC 5 cut(s) 445, 569, 1418, 1494, 1653
PpsI GAGTC 5 cut(s) 445, 569, 1418, 1494, 1653
Ppu21I YACGTR 1 cut(s) 320
PpuMI RGGWCCY 2 cut(s) 284, 345
Psp1406I AACGTT 1 cut(s) 1015
Psp5II RGGWCCY 2 cut(s) 284, 345
Psp6I CCWGG 4 cut(s) 712, 1963, 2050, 2219
PspFI CCCAGC 1 cut(s) 796
PspGI CCWGG 4 cut(s) 712, 1963, 2050, 2219
PspN4I GGNNCC 8 cut(s) 39, 285, 347, 358, 1003, 1149, 2218, 2391
PspPI GGNCC 8 cut(s) 38, 284, 345, 357, 1486, 1607, 1893, 2336
PspPPI RGGWCCY 2 cut(s) 284, 345
PstI CTGCAG 3 cut(s) 391, 603, 2293
PsuI RGATCY 5 cut(s) 838, 904, 1030, 1147, 1736
PvuII CAGCTG 4 cut(s) 796, 824, 1547, 2333
RruI TCGCGA 1 cut(s) 1668
RsaI GTAC 6 cut(s) 1117, 1328, 1356, 1543, 1573, 2240
RsaNI GTAC 6 cut(s) 1116, 1327, 1355, 1542, 1572, 2239
RseI CAYNNNNRTG 2 cut(s) 2159, 2360
SapI GCTCTTC 1 cut(s) 1443
SaqAI TTAA 2 cut(s) 2322, 2358
Sau96I GGNCC 8 cut(s) 38, 284, 345, 357, 1486, 1607, 1893, 2336
ScaI AGTACT 2 cut(s) 1328, 1573
SchI GAGTC 5 cut(s) 446, 570, 1419, 1494, 1654
ScrFI CCNGG 4 cut(s) 714, 1965, 2052, 2221
SduI GDGCHC 2 cut(s) 187, 1865
SexAI ACCWGGT 2 cut(s) 712, 2050
SfaNI GCATC 6 cut(s) 301, 1299, 1678, 1760, 2030, 2036
SfcI CTRYAG 8 cut(s) 387, 477, 599, 1548, 2013, 2064, 2076, 2289
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 5 cut(s) 284, 345, 357, 1607, 2336
SmiMI CAYNNNNRTG 2 cut(s) 2159, 2360
SmlI CTYRAG 2 cut(s) 1163, 1920
SmoI CTYRAG 2 cut(s) 1163, 1920
SphI GCATGC 1 cut(s) 1567
SsiI CCGC 5 cut(s) 465, 573, 804, 1530, 1960
SspMI CTAG 3 cut(s) 567, 882, 939
StyD4I CCNGG 4 cut(s) 712, 1963, 2050, 2219
StyI CCWWGG 2 cut(s) 881, 1005
TaaI ACNGT 5 cut(s) 478, 1186, 1251, 2173, 2308
TaiI ACGT 5 cut(s) 322, 1018, 2005, 2210, 2352
TaqI TCGA 3 cut(s) 106, 559, 2244
TatI WGTACW 3 cut(s) 1326, 1571, 2238
TauI GCSGC 2 cut(s) 575, 1963
TfiI GAWTC 3 cut(s) 886, 1124, 1427
Tru1I TTAA 2 cut(s) 2322, 2358
Tru9I TTAA 2 cut(s) 2322, 2358
TscAI CASTG 5 cut(s) 603, 988, 1303, 2178, 2313
TseFI GTSAC 1 cut(s) 2293
TseI GCWGC 9 cut(s) 386, 482, 821, 935, 968, 1577, 1651, 1742, 2288
Tsp45I GTSAC 1 cut(s) 2293
TspDTI ATGAA 3 cut(s) 737, 1033, 1077
TspRI CASTG 5 cut(s) 603, 988, 1303, 2178, 2313
VpaK11BI GGWCC 5 cut(s) 284, 345, 357, 1607, 2336
XapI RAATTY 3 cut(s) 31, 108, 864
XceI RCATGY 2 cut(s) 76, 1567
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmaJI CCTAGG 1 cut(s) 881
XmnI GAANNNNTTC 1 cut(s) 1662
XspI CTAG 3 cut(s) 567, 882, 939
ZrmI AGTACT 2 cut(s) 1328, 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.