MD17G1161300.v1.1

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005820
Physical Location & Seq
Forward (+)
15870142 .. 15883356
13215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1161300.v1.1.491

Sequence Viewer

Length: 2403 bp
ATGGATGTTGATGTGGAGGTTGGTAATTTGGGGCCTTACCAAGACAGGCCGAGGACATTCCCTAACATGCGTGGTAAATCTTACAACCCATTGATTTTCCGGATTCTGATGCGGATAAATGTCCGTGTTCTTTTTGTTATTTTACTTCTTGCACTTGGAGTGGTCTTTTACGTTGGAGCAAGTACTTCCCCTATCATTGTGTTCGTTTTCTCAACTTGTATTATCAGCTTTTTGGTGTCTATGTACCTGGCTAAATGGGTTCTCGCAAAGGATGAGGGACCTCCGGAGATGGGTCAGATATCAGATGCAATACGTGATGGAGCTGAAGGTTTCTTCAGGACCCAGTATGGAACCATCTCTAAGATGGCAATTTTACTAGCTTCGGTTATCCTCTTCATATATTTATTTCGCAGACCAACACCTCAGCAAGAAGCATCTGGTCTTGGGAGGTTCACTTCAGCTTATATTACAGTAGCTGCATTTCTTTTGGGAGCTTTGTGTTCAGGTGCTGCTGGGTATGTTGGGATGTGGGTTTCAGTTCGTGCAAATGTTCGAGTCTCTAGTGCTGCTAGACGGTCTGCAAGAGAGGCATTACAGATAGCTGTTCGTGCTGGTGGTTTTTCTGCTTTGGTGGTTGTTGGTATGGCTGTACTTGGTATAACCATCCTGTATGCCATTTTTTATGTTTGGTTGGAAGTGGATTCACCAGGTTCAATGAAGATTACTGACTTGCCTCTTCTGCTTGTTGGATATGGATTTGGAGCTTCTTTTGTTGCCTTATTTGCTCAGTTGGGAGGTGGAATATACACCAAGGCAGCTGATGTTGGAGCAGATCTTGTTGGAAAAGTAGAGCAGGGAATTCCCGAAGATGACCCTAGGAATCCTGCTGTGATTGCTGATCTGGTAGGAGACAATGTGGGAGATTGTGCAGCCCGTGGTGCTGATCTTTTTGAAAGTATTGCTGCTGAAATTATTAGTGCTATGATTCTTGGGGGAACGATGGCTCAACGTTGTAAAATTGAAGATGCATCTGGTTTCATCCTGTTTCCTCTGGTTGTTCACTCTTTTGATCTGGTGATTTCATCGGTTGGAATATTCTCAATTAGAGGTACACGAGATTCCAGTGGAAAGGCTTCTTCTGAGGATCCAATGAAAATTCTACAGAGAGGATATTCTGTTACAATACTCTTGGCTGTTCTGACATTTGGTGCGTCTACGCGATGGCTGCTTTATACTGAGCAAGCACCATCTGCGTGGTTTAACTTTGCCTTGTGTGGGCTAGTTGGCATCATCACAGCATATGTCTTCGTCTTTATCACCCAATACTATACCGACTACAAACACGAGCCTGTACGCACATTAGCTCTTGCAAGCTCCACAGGTCATGGGACTAATATAATTGCTGGAGTCAGTTTGGGTCTTGAATCAACTGCTCTTCCTGTTCTTGTCATTAGTGTATCTATTATCTCAGCTTACTGGCTCGGCCAGACCTCTGGCCTTATAGACGAAAATGGAATTCCAACTGGTGGGCTATTTGGCACAGCTGTAGCAACAATGGGAATGCTCAGTACTGCTGCATATGTTCTCACTATGGATATGTTTGGTCCAATAGCTGATAATGCTGGTGGAATTGTAGAGATGAGTCAGCAGCCTGAAAGTGTTCGGGAGATCACTGATGTTCTAGACGCAGTAGGAAACACTACAAAAGCTACCACTAAAGGATTTGCCATCGGATCTGCTGCACTTGCATCTTTTCTTCTGTTTAGTGCTTATATGGACGAGGTTGCTGCCTTTGCACGTGAACCTTTCAAACAGGTTGATATTGCCATTCCAGAAGTTTTTGTTGGCGGATTGTTGGGCTCTATGCTGATATTTCTATTTAGCGCGTGGGCCTGTTCAGCAGTTGGCCGAACTGCACAAGAGGTTGTTAAGGAAGTAAGGCGGCAATTTATTGAGAGGCCTGGGATAATGGAGTACAAGGAGAAGCCAGATTATGCTCGTTGTGTCGCGATTGTAGCATCTGCGTCTTTAAGGGAGATGATAAAACCTGGTGTCTTGGCTATTATTTCACCTATAGCTGTTGGTATTGTGTTCCGGATCTTTGGATACTACAATGGGCAACCTCTACTTGGTGCTAAAGTTGTGGCTTCCATGCTGATGTTTGCAACTGTTTCCGGTATTCTTATGGCACTTTTCCTCAACACAGCCGGAGGTGCCTGGGATAATGCAAAGAAGTACATTGAGACAGGGGTTCTCGGTGGCAAAGGAAGTGATTGCCATAAGGCTGCAGTTACAGGAGATACTGTGGGTGACCCATTCAAAGACACAGCCGGACCTTCGCTGCACGTTCTCATCAAAATGCTTGCAACTATAACGCTGGTTATGGCTCCGGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

801

Amino Acids

85.15

Weight (kDa)

5.78

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 74 - 795 7.4e-258 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2213
AccB7I CCANNNNNTGG 1 cut(s) 1526
AccI GTMKAC 1 cut(s) 1214
AccII CGCG 3 cut(s) 1219, 1886, 2009
AccIII TCCGGA 3 cut(s) 99, 283, 2094
AciI CCGC 3 cut(s) 112, 1848, 1942
AclI AACGTT 1 cut(s) 1009
AclWI GGATC 4 cut(s) 1139, 1152, 1741, 2105
AcoI YGGCCR 2 cut(s) 1483, 1906
AcsI RAATTY 3 cut(s) 858, 1155, 1515
AcuI CTGAAG 3 cut(s) 319, 345, 441
AcvI CACGTG 1 cut(s) 1799
AfaI GTAC 8 cut(s) 184, 245, 651, 1111, 1353, 1570, 1976, 2237
AfiI CCNNNNNNNGG 4 cut(s) 290, 1275, 1526, 2129
AgsI TTSAA 6 cut(s) 714, 953, 1022, 1424, 1810, 2320
AjnI CCWGG 5 cut(s) 246, 706, 1960, 2047, 2216
AjuI GAANNNNNNNTTGG 2 cut(s) 1827, 1859
Alw26I GTCTC 3 cut(s) 562, 903, 2237
AlwI GGATC 4 cut(s) 1139, 1152, 1741, 2105
AlwNI CAGNNNCTG 2 cut(s) 476, 509
Aor13HI TCCGGA 3 cut(s) 99, 283, 2094
AoxI GGCC 7 cut(s) 32, 47, 1483, 1495, 1890, 1906, 1958
ApoI RAATTY 3 cut(s) 858, 1155, 1515
Asp700I GAANNNNTTC 2 cut(s) 1132, 1659
AspA2I CCTAGG 1 cut(s) 875
AspLEI GCGC 1 cut(s) 1886
AspS9I GGNCC 6 cut(s) 32, 278, 339, 1604, 1890, 2333
AsuHPI GGTGA 5 cut(s) 696, 1087, 1309, 2061, 2321
AvaII GGWCC 4 cut(s) 278, 339, 1604, 2333
AvrII CCTAGG 1 cut(s) 875
BamHI GGATCC 1 cut(s) 1144
BanI GGYRCC 1 cut(s) 2213
BanII GRGCYC 1 cut(s) 1862
BauI CACGAG 2 cut(s) 1113, 1343
BbrPI CACGTG 1 cut(s) 1799
BbsI GAAGAC 1 cut(s) 1297
BbvCI CCTCAGC 1 cut(s) 423
BccI CCATC 9 cut(s) 283, 311, 358, 362, 671, 994, 1215, 1255, 1736
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 5 cut(s) 248, 708, 1962, 2049, 2218
BciVI GTATCC 1 cut(s) 2099
BcoDI GTCTC 3 cut(s) 562, 903, 2237
BfaI CTAG 6 cut(s) 377, 561, 570, 876, 1280, 1682
BfmI CTRYAG 4 cut(s) 1160, 1545, 2073, 2286
BfuI GTATCC 1 cut(s) 2099
BglII AGATCT 1 cut(s) 832
BlnI CCTAGG 1 cut(s) 875
BmcAI AGTACT 2 cut(s) 184, 1570
Bme1390I CCNGG 5 cut(s) 248, 708, 1962, 2049, 2218
Bme18I GGWCC 4 cut(s) 278, 339, 1604, 2333
BmgT120I GGNCC 6 cut(s) 32, 278, 339, 1604, 1890, 2333
BmiI GGNNCC 7 cut(s) 33, 279, 341, 352, 1146, 2215, 2388
BmrFI CCNGG 5 cut(s) 248, 708, 1962, 2049, 2218
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 8 cut(s) 99, 295, 443, 1015, 1037, 1296, 1757, 2027
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 1297
BpmI CTGGAG 1 cut(s) 1425
Bpu10I CCTNAGC 1 cut(s) 423
BsaAI YACGTR 2 cut(s) 314, 1799
BsaJI CCNNGG 6 cut(s) 50, 810, 875, 934, 1961, 2217
BsaWI WCCGGW 5 cut(s) 99, 283, 2094, 2174, 2389
BsaXI ACNNNNNCTCC 4 cut(s) 900, 930, 2289, 2319
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 1275, 1526, 2129
Bse1I ACTGG 4 cut(s) 343, 1122, 1481, 1528
BseAI TCCGGA 3 cut(s) 99, 283, 2094
BseBI CCWGG 5 cut(s) 248, 708, 1962, 2049, 2218
BseDI CCNNGG 6 cut(s) 50, 810, 875, 934, 1961, 2217
BseGI GGATG 5 cut(s) 10, 277, 531, 663, 1038
BseLI CCNNNNNNNGG 4 cut(s) 290, 1275, 1526, 2129
BseMII CTCAG 6 cut(s) 437, 800, 1131, 1227, 1482, 1579
BseNI ACTGG 4 cut(s) 343, 1122, 1481, 1528
BseYI CCCAGC 1 cut(s) 512
BsgI GTGCAG 4 cut(s) 948, 1725, 1899, 2327
Bsh1236I CGCG 3 cut(s) 1219, 1886, 2009
BshFI GGCC 7 cut(s) 34, 49, 1485, 1497, 1892, 1908, 1960
BshNI GGYRCC 1 cut(s) 2213
BsiSI CCGG 7 cut(s) 100, 284, 2095, 2175, 2208, 2331, 2390
BslFI GGGAC 2 cut(s) 291, 1402
BslI CCNNNNNNNGG 4 cut(s) 290, 1275, 1526, 2129
BsmAI GTCTC 3 cut(s) 562, 903, 2237
BsmFI GGGAC 2 cut(s) 291, 1402
BsmI GAATGC 1 cut(s) 1566
BsnI GGCC 7 cut(s) 34, 49, 1485, 1497, 1892, 1908, 1960
Bsp1286I GDGCHC 1 cut(s) 1862
Bsp13I TCCGGA 3 cut(s) 99, 283, 2094
Bsp143I GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
Bsp68I TCGCGA 1 cut(s) 2009
BspACI CCGC 3 cut(s) 112, 1848, 1942
BspANI GGCC 7 cut(s) 34, 49, 1485, 1497, 1892, 1908, 1960
BspCNI CTCAG 6 cut(s) 436, 799, 1132, 1228, 1481, 1578
BspEI TCCGGA 3 cut(s) 99, 283, 2094
BspFNI CGCG 3 cut(s) 1219, 1886, 2009
BspLI GGNNCC 7 cut(s) 33, 279, 341, 352, 1146, 2215, 2388
BspMAI CTGCAG 1 cut(s) 2290
BspPI GGATC 4 cut(s) 1139, 1152, 1741, 2105
BspQI GCTCTTC 1 cut(s) 1440
BspT107I GGYRCC 1 cut(s) 2213
BsrI ACTGG 4 cut(s) 343, 1122, 1481, 1528
BssECI CCNNGG 6 cut(s) 50, 810, 875, 934, 1961, 2217
BssMI GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
BssSI CACGAG 2 cut(s) 1113, 1343
BssT1I CCWWGG 2 cut(s) 810, 875
Bst2BI CACGAG 2 cut(s) 1113, 1343
Bst2UI CCWGG 5 cut(s) 248, 708, 1962, 2049, 2218
Bst4CI ACNGT 4 cut(s) 472, 576, 2170, 2305
Bst6I CTCTTC 3 cut(s) 398, 741, 1440
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstBAI YACGTR 2 cut(s) 314, 1799
BstC8I GCNNGC 3 cut(s) 1242, 1372, 2364
BstDEI CTNAG 7 cut(s) 360, 423, 786, 1140, 1236, 1468, 1565
BstDSI CCRYGG 1 cut(s) 934
BstEII GGTNACC 1 cut(s) 2309
BstF5I GGATG 5 cut(s) 10, 277, 531, 663, 1038
BstFNI CGCG 3 cut(s) 1219, 1886, 2009
BstHHI GCGC 1 cut(s) 1886
BstKTI GATC 8 cut(s) 835, 901, 946, 1072, 1147, 1671, 1736, 2100
BstMAI GTCTC 3 cut(s) 562, 903, 2237
BstMBI GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
BstNI CCWGG 5 cut(s) 248, 708, 1962, 2049, 2218
BstNSI RCATGY 1 cut(s) 70
BstPI GGTNACC 1 cut(s) 2309
BstSCI CCNGG 5 cut(s) 246, 706, 1960, 2047, 2216
BstSFI CTRYAG 4 cut(s) 1160, 1545, 2073, 2286
BstUI CGCG 3 cut(s) 1219, 1886, 2009
BstV2I GAAGAC 1 cut(s) 1297
BstX2I RGATCY 4 cut(s) 832, 1144, 1733, 2097
BstXI CCANNNNNNTGG 2 cut(s) 1254, 1493
BstYI RGATCY 4 cut(s) 832, 1144, 1733, 2097
BsuI GTATCC 1 cut(s) 2099
BsuRI GGCC 7 cut(s) 34, 49, 1485, 1497, 1892, 1908, 1960
BtgI CCRYGG 1 cut(s) 934
BtgZI GCGATG 1 cut(s) 1234
BtsCI GGATG 5 cut(s) 10, 277, 531, 663, 1038
BtsIMutI CAGTG 2 cut(s) 1129, 1671
BtuMI TCGCGA 1 cut(s) 2009
Cac8I GCNNGC 3 cut(s) 1242, 1372, 2364
CaiI CAGNNNCTG 2 cut(s) 476, 509
CfoI GCGC 1 cut(s) 1886
Cfr13I GGNCC 6 cut(s) 32, 278, 339, 1604, 1890, 2333
CseI GACGC 3 cut(s) 1200, 1694, 2013
CsiI ACCWGGT 2 cut(s) 706, 2047
Csp6I GTAC 8 cut(s) 183, 244, 650, 1110, 1352, 1569, 1975, 2236
CviAII CATG 3 cut(s) 67, 1385, 2152
CviQI GTAC 8 cut(s) 183, 244, 650, 1110, 1352, 1569, 1975, 2236
DdeI CTNAG 7 cut(s) 360, 423, 786, 1140, 1236, 1468, 1565
DpnI GATC 8 cut(s) 834, 900, 945, 1071, 1146, 1670, 1735, 2099
DpnII GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
EaeI YGGCCR 2 cut(s) 1483, 1906
Eam1104I CTCTTC 3 cut(s) 398, 741, 1440
EarI CTCTTC 3 cut(s) 398, 741, 1440
EciI GGCGGA 1 cut(s) 1863
Eco130I CCWWGG 2 cut(s) 810, 875
Eco147I AGGCCT 1 cut(s) 1960
Eco24I GRGCYC 1 cut(s) 1862
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 4 cut(s) 278, 339, 1604, 2333
Eco57I CTGAAG 3 cut(s) 319, 345, 441
Eco72I CACGTG 1 cut(s) 1799
Eco91I GGTNACC 1 cut(s) 2309
EcoO109I RGGNCCY 3 cut(s) 32, 278, 339
EcoO65I GGTNACC 1 cut(s) 2309
EcoRI GAATTC 2 cut(s) 858, 1515
EcoRII CCWGG 5 cut(s) 246, 706, 1960, 2047, 2216
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 2 cut(s) 810, 875
EcoT22I ATGCAT 1 cut(s) 1030
EcoT38I GRGCYC 1 cut(s) 1862
ErhI CCWWGG 2 cut(s) 810, 875
FaeI CATG 3 cut(s) 70, 1388, 2155
FaqI GGGAC 2 cut(s) 291, 1402
FatI CATG 3 cut(s) 66, 1384, 2151
FauNDI CATATG 2 cut(s) 1300, 1579
FblI GTMKAC 1 cut(s) 1214
FokI GGATG 5 cut(s) 17, 284, 538, 650, 1025
FriOI GRGCYC 1 cut(s) 1862
FspBI CTAG 6 cut(s) 377, 561, 570, 876, 1280, 1682
GlaI GCGC 1 cut(s) 1885
GsaI CCCAGC 1 cut(s) 516
GsuI CTGGAG 1 cut(s) 1425
HaeIII GGCC 7 cut(s) 34, 49, 1485, 1497, 1892, 1908, 1960
HapII CCGG 7 cut(s) 100, 284, 2095, 2175, 2208, 2331, 2390
HgaI GACGC 3 cut(s) 1200, 1694, 2013
HhaI GCGC 1 cut(s) 1886
Hin1II CATG 3 cut(s) 70, 1388, 2155
Hin6I GCGC 1 cut(s) 1884
HinP1I GCGC 1 cut(s) 1884
HinfI GANTC 9 cut(s) 103, 555, 701, 880, 985, 1118, 1407, 1424, 1642
HpaII CCGG 7 cut(s) 100, 284, 2095, 2175, 2208, 2331, 2390
HphI GGTGA 5 cut(s) 696, 1087, 1309, 2061, 2321
Hpy166II GTNNAC 5 cut(s) 453, 1060, 1112, 1215, 1802
Hpy188I TCNGA 7 cut(s) 108, 297, 304, 1141, 1200, 1733, 2402
Hpy8I GTNNAC 5 cut(s) 453, 1060, 1112, 1215, 1802
HpyAV CCTTC 2 cut(s) 320, 2346
HpyCH4III ACNGT 4 cut(s) 472, 576, 2170, 2305
HpyCH4IV ACGT 5 cut(s) 171, 313, 1009, 1798, 2346
HpyF3I CTNAG 7 cut(s) 360, 423, 786, 1140, 1236, 1468, 1565
HpySE526I ACGT 5 cut(s) 171, 313, 1009, 1798, 2346
Hsp92II CATG 3 cut(s) 70, 1388, 2155
HspAI GCGC 1 cut(s) 1884
Kpn2I TCCGGA 3 cut(s) 99, 283, 2094
Kzo9I GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
LguI GCTCTTC 1 cut(s) 1440
LmnI GCTCC 7 cut(s) 176, 320, 491, 761, 827, 1379, 2392
LweI GCATC 8 cut(s) 99, 295, 443, 1015, 1037, 1296, 1757, 2027
MabI ACCWGGT 2 cut(s) 706, 2047
MaeI CTAG 6 cut(s) 377, 561, 570, 876, 1280, 1682
MaeII ACGT 5 cut(s) 171, 313, 1009, 1798, 2346
MaeIII GTNAC 3 cut(s) 1177, 2290, 2309
MalI GATC 8 cut(s) 834, 900, 945, 1071, 1146, 1670, 1735, 2099
MboI GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
MflI RGATCY 4 cut(s) 832, 1144, 1733, 2097
MhlI GDGCHC 1 cut(s) 1862
MlyI GAGTC 3 cut(s) 564, 1416, 1651
MmeI TCCRAC 7 cut(s) 154, 672, 727, 805, 820, 1069, 1544
Mph1103I ATGCAT 1 cut(s) 1030
MroI TCCGGA 3 cut(s) 99, 283, 2094
MroXI GAANNNNTTC 2 cut(s) 1132, 1659
MseI TTAA 3 cut(s) 1260, 1929, 2030
MslI CAYNNNNRTG 3 cut(s) 1252, 2156, 2357
MspA1I CMGCKG 2 cut(s) 818, 1544
MspI CCGG 7 cut(s) 100, 284, 2095, 2175, 2208, 2331, 2390
MspR9I CCNGG 5 cut(s) 248, 708, 1962, 2049, 2218
Mva1269I GAATGC 1 cut(s) 1566
MvaI CCWGG 5 cut(s) 248, 708, 1962, 2049, 2218
MvnI CGCG 3 cut(s) 1219, 1886, 2009
NdeI CATATG 2 cut(s) 1300, 1579
NdeII GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
NlaIII CATG 3 cut(s) 70, 1388, 2155
NlaIV GGNNCC 7 cut(s) 33, 279, 341, 352, 1146, 2215, 2388
NmeAIII GCCGAG 2 cut(s) 75, 1461
NmuCI GTSAC 1 cut(s) 2309
NruI TCGCGA 1 cut(s) 2009
NsiI ATGCAT 1 cut(s) 1030
NspI RCATGY 1 cut(s) 70
PceI AGGCCT 1 cut(s) 1960
PciSI GCTCTTC 1 cut(s) 1440
PctI GAATGC 1 cut(s) 1566
PdmI GAANNNNTTC 2 cut(s) 1132, 1659
PfeI GAWTC 6 cut(s) 103, 701, 880, 985, 1118, 1424
PflMI CCANNNNNTGG 1 cut(s) 1526
PleI GAGTC 3 cut(s) 563, 1415, 1650
PmaCI CACGTG 1 cut(s) 1799
PmlI CACGTG 1 cut(s) 1799
PpsI GAGTC 3 cut(s) 563, 1415, 1650
Ppu21I YACGTR 2 cut(s) 314, 1799
PpuMI RGGWCCY 2 cut(s) 278, 339
Psp1406I AACGTT 1 cut(s) 1009
Psp5II RGGWCCY 2 cut(s) 278, 339
Psp6I CCWGG 5 cut(s) 246, 706, 1960, 2047, 2216
PspCI CACGTG 1 cut(s) 1799
PspEI GGTNACC 1 cut(s) 2309
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 5 cut(s) 246, 706, 1960, 2047, 2216
PspN4I GGNNCC 7 cut(s) 33, 279, 341, 352, 1146, 2215, 2388
PspPI GGNCC 6 cut(s) 32, 278, 339, 1604, 1890, 2333
PspPPI RGGWCCY 2 cut(s) 278, 339
PstI CTGCAG 1 cut(s) 2290
PstNI CAGNNNCTG 2 cut(s) 476, 509
PsuI RGATCY 4 cut(s) 832, 1144, 1733, 2097
PvuII CAGCTG 2 cut(s) 818, 1544
RruI TCGCGA 1 cut(s) 2009
RsaI GTAC 8 cut(s) 184, 245, 651, 1111, 1353, 1570, 1976, 2237
RsaNI GTAC 8 cut(s) 183, 244, 650, 1110, 1352, 1569, 1975, 2236
RseI CAYNNNNRTG 3 cut(s) 1252, 2156, 2357
SapI GCTCTTC 1 cut(s) 1440
SaqAI TTAA 3 cut(s) 1260, 1929, 2030
Sau3AI GATC 8 cut(s) 832, 898, 943, 1069, 1144, 1668, 1733, 2097
Sau96I GGNCC 6 cut(s) 32, 278, 339, 1604, 1890, 2333
ScaI AGTACT 2 cut(s) 184, 1570
SchI GAGTC 3 cut(s) 564, 1416, 1651
ScrFI CCNGG 5 cut(s) 248, 708, 1962, 2049, 2218
SduI GDGCHC 1 cut(s) 1862
SexAI ACCWGGT 2 cut(s) 706, 2047
SfaNI GCATC 8 cut(s) 99, 295, 443, 1015, 1037, 1296, 1757, 2027
SfcI CTRYAG 4 cut(s) 1160, 1545, 2073, 2286
SinI GGWCC 4 cut(s) 278, 339, 1604, 2333
SmiMI CAYNNNNRTG 3 cut(s) 1252, 2156, 2357
SseBI AGGCCT 1 cut(s) 1960
SsiI CCGC 3 cut(s) 112, 1848, 1942
SspI AATATT 1 cut(s) 1095
SspMI CTAG 6 cut(s) 377, 561, 570, 876, 1280, 1682
StuI AGGCCT 1 cut(s) 1960
StyD4I CCNGG 5 cut(s) 246, 706, 1960, 2047, 2216
StyI CCWWGG 2 cut(s) 810, 875
TaaI ACNGT 4 cut(s) 472, 576, 2170, 2305
TaiI ACGT 5 cut(s) 174, 316, 1012, 1801, 2349
TaqI TCGA 1 cut(s) 553
TatI WGTACW 5 cut(s) 182, 649, 1568, 1974, 2235
TauI GCSGC 1 cut(s) 1945
TfiI GAWTC 6 cut(s) 103, 701, 880, 985, 1118, 1424
Tru1I TTAA 3 cut(s) 1260, 1929, 2030
Tru9I TTAA 3 cut(s) 1260, 1929, 2030
TscAI CASTG 2 cut(s) 1129, 1678
TseFI GTSAC 1 cut(s) 2309
Tsp45I GTSAC 1 cut(s) 2309
TspDTI ATGAA 5 cut(s) 385, 731, 1027, 1071, 1166
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 1129, 1678
Van91I CCANNNNNTGG 1 cut(s) 1526
VpaK11BI GGWCC 4 cut(s) 278, 339, 1604, 2333
XapI RAATTY 3 cut(s) 858, 1155, 1515
XbaI TCTAGA 1 cut(s) 1681
XceI RCATGY 1 cut(s) 70
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XmaJI CCTAGG 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 1214
XmnI GAANNNNTTC 2 cut(s) 1132, 1659
XspI CTAG 6 cut(s) 377, 561, 570, 876, 1280, 1682
ZrmI AGTACT 2 cut(s) 184, 1570
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.