RLG00000020371

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005820
Physical Location & Seq
Forward (+)
64302366 .. 64309099
6734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020371

Sequence Viewer

Length: 2577 bp
ATGGCCTGCGCCACGTCGGCAAGGAACGACGACTTGGGCACCCTCCTCCTCCTCCTCCTCCTCCTCCTTTGTGCTGTTGAAACTCGGCCTGGTTCAGGGGGCGATTTGCCCCAGGCTAGCGAGTACAGTCCTTTTCCTGTAGTTCGGAGGGTGAATTCCGGCCATACAATGCTCATGGGTGGTGATTTGGAGGATGGTAGTTTGGGGCCTTACCAAGACAGGCTGAGGACATTTCCAAACATGCGTGGTAAATCTTACAACCCATTGATTTTTCGAATTTTGATGCGAATAAATGTTCGTGTCCTTTTCGTGATTTTGCTTCTGGCACTTGGAGTGGTCTTTTACATTGGAGCAAGCACTTCTCCTATCATTGTGTTCGTCTTCTCAGTTTGTATTATCAGCTTTCTTCTGGCAATGCACCTTGCCAAATGGGTTCTTGCAAAAGATGAGGGACCTCCGGAGATGGGTCAGATATCAGATGCAATACGTGATGGAGCTGAAGGTTTCTTCAGGACCCAGTATGGGACCATCTCTAAGATGGCATTTTTACTGGCTGCGGTTATCCTTTGCATATACTTATTTCGCACTACAACTCCTCAGCAAGAGTCATCTGGCCTTGGAAGGGTAGCCACTGCATGTATTACTGTAGCTGCATTTCTTTTGGGAGCTTTCTGTTCAGGTATTGCAGGGTATGTTGGGATGTGGGTATCAGTTCGTGCCAATGTTCGAGTCTCTAGTGCTGCAAGACGGTCTGCAAGGGAGGCATTGCAGATAGCTGTTCGTGCTGGTGGTTTTTCTGCTATGATTGTCGTTGGTATGGCTGTAATCGGTATTGCCATCCTGTATTCCACATTTTATGTCTGGCTGGGAGTGGATACACCGGGTTCAATGAAGGTTACTGACTTGCCTCTTCTTCTTGTTGGATACGGCTTTGGAGCTTCTTTTGTTGCCTTATTTGCTCAGCTGGGTGGGGGAATATACACAAAAGCAGCTGATGTTGGAGCAGATCTTGTTGGAAAAGTTGAGCAGGGGATTCCTGAAGATGATCCTAGGAATCCTGCTGTGATAGCAGATCTGGTTGGAGATAATGTCGGGGATTGCGCGGCTAGAGGTGCTGATCTTTTTGAAAGTATTGCTGCTGAAATAATCAGTGCTATGATACTTGGGGGAACAATGGCTCAACGTTGTAAAATTGAAGATCCTTCTGGTTTCATCTTGTTTCCTCTGGTTGTTCACTCTTTTGATCTGGTGATTTCATCGGTTGGAATACTTTCAATCAGGGGTACACGAGATTCTGGTGCGAAGGGTTCTACAGAGGATCCAATGACAATTCTCGAGCGAGGATACTCAGTCACAGTAGTTTTAGCTGTTCTGACATTTGGTGCGTCGACCCGATGGTTATTATACACTGAACAAGCACCGTCTGCATGGTTTCACTTTGCTTTATGTGGATTAGTTGGCATCATCACTGCATATATTTTTGTGTGGATAACCAAGTACTATACAGACTACAAGTATGAGCCTGTACGCACATTAGCTCTTGCGAGCTCCACAGGGCATGGGACTAATATAATCGCTGGAGTCAGTTTGGGTCTGGAATCAACAGCTCTTCCTGTTCTTGTCATTAGCGTAGCTATTGTTTCAGCTTACTGGCTGGGCCAGACCTCTGGACTCATGGACGAAAATGGTGTTCCTACTGGCGGGCTGTTTGGCACAGCTGTAGCAACAATGGGCATGCTAAGTACTGCTGCTTATGTTCTCACGATGGATATGTTTGGTCCAATAGCTGATAATGCTGGTGGAATTGTAGAGATGAGTCAACAGCCAGAAAGTGTTCGCGAGATCACCGATGTTCTTGATGCAGTAGGAAACACTACAAAAGCTACCACTAAAGGGTTTGCCATTGGATCTGCTGCACTTGCATCTTTTCTTCTGTTTAGTGCTTATATGGATGAGGTAGCTACATTTGCCCGTAAACCTTTCAAGCAGGTTGATATTGCCATTCCAGAAGTTTTTGTTGGCGGATTGTTGGGCTCTATGCTTATTTTCTTATTTAGTGCCTGGGCCTGTTCAGCAGTTGGCCGAACTGCTCAAGAGGTTGTCAGGGAAGTAAGAAGGCAATTTATTGAGAGGCCTGGTATAATGGAGTATAAGGAGAAGCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTGCATCTTTGAAGGAGATGATAAAACCTGGTGCCTTGGCTATTGTTTCACCTATAGCAGTTGGTATTACGTTCCGGATATTTGGATACTATACTGGGCAGCCTTTACTTGGTGCTAAAGTTGTGGCTTCCATGCTGATGTTTGCAACGGTGTCGGGTATTCTCATGGCTCTTTTCCTGAACACGGCAGGTGGTGCCTGGGATAACGCAAAGAAGTACATCGAGACAGGGGTTCTCGGAGGCAAAGGAAGTGATTGTCATAAGGCTGCAGTTACAGGAGACACAGTGGGAGACCCATTCAAAGACACAGCTGGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAATAACACTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

859

Amino Acids

90.98

Weight (kDa)

5.94

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 132 - 853 8.3e-255 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2372
Acc36I ACCTGC 2 cut(s) 1978, 2372
AccB1I GGYRCC 3 cut(s) 38, 2225, 2387
AccI GTMKAC 1 cut(s) 1388
AccII CGCG 2 cut(s) 1103, 1839
AccIII TCCGGA 2 cut(s) 457, 2268
AciI CCGC 4 cut(s) 557, 1103, 1701, 2022
AclI AACGTT 1 cut(s) 1183
AclWI GGATC 5 cut(s) 1040, 1193, 1313, 1326, 1915
AcoI YGGCCR 2 cut(s) 160, 2080
AcsI RAATTY 2 cut(s) 154, 276
AcuI CTGAAG 3 cut(s) 493, 519, 1059
AdeI CACNNNGTG 1 cut(s) 2177
AfaI GTAC 6 cut(s) 125, 1285, 1499, 1527, 1744, 2411
AfiI CCNNNNNNNGG 8 cut(s) 95, 464, 522, 622, 1700, 1893, 2303, 2377
AgsI TTSAA 8 cut(s) 80, 888, 1127, 1196, 1275, 1984, 2206, 2494
AjiI CACGTC 1 cut(s) 15
AjnI CCWGG 6 cut(s) 88, 111, 2060, 2134, 2221, 2390
AjuI GAANNNNNNNTTGG 4 cut(s) 17, 49, 2001, 2033
Alw21I GWGCWC 1 cut(s) 1550
Alw26I GTCTC 4 cut(s) 736, 2411, 2466, 2478
AlwI GGATC 5 cut(s) 1040, 1193, 1313, 1326, 1915
Ama87I CYCGRG 1 cut(s) 1334
Aor13HI TCCGGA 2 cut(s) 457, 2268
AoxI GGCC 9 cut(s) 3, 86, 160, 206, 613, 1657, 2064, 2080, 2132
ApeKI GCWGC 9 cut(s) 554, 650, 740, 989, 1136, 1748, 1913, 2293, 2459
ApoI RAATTY 2 cut(s) 154, 276
Asp700I GAANNNNTTC 2 cut(s) 1270, 1833
AspA2I CCTAGG 1 cut(s) 1049
AspLEI GCGC 2 cut(s) 11, 1103
AspS9I GGNCC 8 cut(s) 206, 452, 513, 525, 1657, 1778, 2064, 2507
AsuC2I CCSGG 1 cut(s) 882
AsuHPI GGTGA 5 cut(s) 163, 194, 1261, 1837, 2235
AsuII TTCGAA 1 cut(s) 274
AsuNHI GCTAGC 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 1334
AvaII GGWCC 5 cut(s) 452, 513, 525, 1778, 2507
AvrII CCTAGG 1 cut(s) 1049
BaeGI GKGCMC 1 cut(s) 41
BamHI GGATCC 1 cut(s) 1318
BanI GGYRCC 3 cut(s) 38, 2225, 2387
BanII GRGCYC 2 cut(s) 1550, 2036
BauI CACGAG 1 cut(s) 1287
BbsI GAAGAC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 1550
BbvCI CCTCAGC 2 cut(s) 224, 597
BbvI GCAGC 9 cut(s) 541, 637, 727, 1001, 1123, 1735, 1900, 2305, 2446
BccI CCATC 8 cut(s) 188, 457, 485, 532, 536, 845, 1389, 1759
BceAI ACGGC 2 cut(s) 943, 2394
BcgI CGANNNNNNTGC 2 cut(s) 2328, 2362
BciT130I CCWGG 6 cut(s) 90, 113, 2062, 2136, 2223, 2392
BciVI GTATCC 4 cut(s) 868, 917, 1337, 2273
BcnI CCSGG 1 cut(s) 882
BcoDI GTCTC 4 cut(s) 736, 2411, 2466, 2478
BfaI CTAG 4 cut(s) 117, 735, 1050, 1107
BfmI CTRYAG 6 cut(s) 138, 645, 1311, 1719, 2247, 2460
BfuAI ACCTGC 2 cut(s) 1978, 2372
BfuI GTATCC 4 cut(s) 868, 917, 1337, 2273
BglI GCCNNNNNGGC 1 cut(s) 17
BglII AGATCT 2 cut(s) 1006, 1072
BlnI CCTAGG 1 cut(s) 1049
BlpI GCTNAGC 1 cut(s) 960
BmcAI AGTACT 2 cut(s) 1499, 1744
Bme1390I CCNGG 7 cut(s) 90, 113, 882, 2062, 2136, 2223, 2392
Bme18I GGWCC 5 cut(s) 452, 513, 525, 1778, 2507
BmeT110I CYCGRG 1 cut(s) 1334
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 8 cut(s) 206, 452, 513, 525, 1657, 1778, 2064, 2507
BmiI GGNNCC 9 cut(s) 40, 207, 453, 515, 526, 1320, 2227, 2389, 2562
BmrFI CCNGG 7 cut(s) 90, 113, 882, 2062, 2136, 2223, 2392
BmrI ACTGGG 2 cut(s) 511, 2298
BmsI GCATC 7 cut(s) 273, 469, 1470, 1849, 1931, 2201, 2207
BmtI GCTAGC 1 cut(s) 120
BmuI ACTGGG 2 cut(s) 511, 2298
BpiI GAAGAC 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 1599
Bpu10I CCTNAGC 2 cut(s) 224, 597
Bpu1102I GCTNAGC 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 2076
BpuMI CCSGG 1 cut(s) 882
BsaAI YACGTR 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 2478
BsaJI CCNNGG 6 cut(s) 111, 616, 1049, 2061, 2229, 2391
BsaWI WCCGGW 2 cut(s) 457, 2268
BsaXI ACNNNNNCTCC 6 cut(s) 577, 607, 1074, 1104, 2463, 2493
Bsc4I CCNNNNNNNGG 8 cut(s) 95, 464, 522, 622, 1700, 1893, 2303, 2377
Bse1I ACTGG 6 cut(s) 517, 555, 1655, 1702, 2293, 2556
Bse3DI GCAATG 2 cut(s) 420, 764
BseAI TCCGGA 2 cut(s) 457, 2268
BseBI CCWGG 6 cut(s) 90, 113, 2062, 2136, 2223, 2392
BseDI CCNNGG 6 cut(s) 111, 616, 1049, 2061, 2229, 2391
BseGI GGATG 4 cut(s) 199, 705, 837, 1957
BseLI CCNNNNNNNGG 8 cut(s) 95, 464, 522, 622, 1700, 1893, 2303, 2377
BseMI GCAATG 2 cut(s) 420, 764
BseMII CTCAG 5 cut(s) 215, 399, 611, 974, 1362
BseNI ACTGG 6 cut(s) 517, 555, 1655, 1702, 2293, 2556
BseRI GAGGAG 8 cut(s) 35, 38, 41, 44, 47, 50, 53, 585
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 9 cut(s) 541, 637, 727, 1001, 1123, 1735, 1900, 2305, 2446
BseYI CCCAGC 3 cut(s) 865, 964, 1654
BsgI GTGCAG 1 cut(s) 1899
Bsh1236I CGCG 2 cut(s) 1103, 1839
BshFI GGCC 9 cut(s) 5, 88, 162, 208, 615, 1659, 2066, 2082, 2134
BshNI GGYRCC 3 cut(s) 38, 2225, 2387
BsiHKAI GWGCWC 1 cut(s) 1550
BsiHKCI CYCGRG 1 cut(s) 1334
BsiSI CCGG 4 cut(s) 159, 458, 881, 2269
BslFI GGGAC 3 cut(s) 465, 538, 1576
BslI CCNNNNNNNGG 8 cut(s) 95, 464, 522, 622, 1700, 1893, 2303, 2377
BsmAI GTCTC 4 cut(s) 736, 2411, 2466, 2478
BsmFI GGGAC 3 cut(s) 465, 538, 1576
BsnI GGCC 9 cut(s) 5, 88, 162, 208, 615, 1659, 2066, 2082, 2134
Bso31I GGTCTC 1 cut(s) 2478
BsoBI CYCGRG 1 cut(s) 1334
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 3 cut(s) 41, 1550, 2036
Bsp13I TCCGGA 2 cut(s) 457, 2268
Bsp143I GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
Bsp1720I GCTNAGC 1 cut(s) 960
Bsp68I TCGCGA 1 cut(s) 1839
BspACI CCGC 4 cut(s) 557, 1103, 1701, 2022
BspANI GGCC 9 cut(s) 5, 88, 162, 208, 615, 1659, 2066, 2082, 2134
BspCNI CTCAG 5 cut(s) 216, 398, 610, 973, 1361
BspEI TCCGGA 2 cut(s) 457, 2268
BspFNI CGCG 2 cut(s) 1103, 1839
BspLI GGNNCC 9 cut(s) 40, 207, 453, 515, 526, 1320, 2227, 2389, 2562
BspMAI CTGCAG 1 cut(s) 2464
BspMI ACCTGC 2 cut(s) 1978, 2372
BspOI GCTAGC 1 cut(s) 120
BspPI GGATC 5 cut(s) 1040, 1193, 1313, 1326, 1915
BspQI GCTCTTC 1 cut(s) 1614
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 3 cut(s) 38, 2225, 2387
BspTNI GGTCTC 1 cut(s) 2478
BsrDI GCAATG 2 cut(s) 420, 764
BsrI ACTGG 6 cut(s) 517, 555, 1655, 1702, 2293, 2556
BssECI CCNNGG 6 cut(s) 111, 616, 1049, 2061, 2229, 2391
BssMI GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
BssSI CACGAG 1 cut(s) 1287
BssT1I CCWWGG 3 cut(s) 616, 1049, 2229
Bst2BI CACGAG 1 cut(s) 1287
Bst2UI CCWGG 6 cut(s) 90, 113, 2062, 2136, 2223, 2392
Bst4CI ACNGT 7 cut(s) 128, 646, 750, 1357, 1422, 2344, 2479
Bst6I CTCTTC 2 cut(s) 915, 1614
BstAPI GCANNNNNTGC 2 cut(s) 1424, 2387
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 7 cut(s) 7, 118, 355, 1546, 1703, 1736, 2538
BstDEI CTNAG 7 cut(s) 224, 385, 534, 597, 960, 1348, 1739
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 199, 705, 837, 1957
BstFNI CGCG 2 cut(s) 1103, 1839
BstHHI GCGC 2 cut(s) 11, 1103
BstKTI GATC 9 cut(s) 1009, 1048, 1075, 1120, 1201, 1246, 1321, 1845, 1910
BstMAI GTCTC 4 cut(s) 736, 2411, 2466, 2478
BstMBI GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
BstNI CCWGG 6 cut(s) 90, 113, 2062, 2136, 2223, 2392
BstNSI RCATGY 3 cut(s) 244, 639, 1738
BstSCI CCNGG 7 cut(s) 88, 111, 880, 2060, 2134, 2221, 2390
BstSFI CTRYAG 6 cut(s) 138, 645, 1311, 1719, 2247, 2460
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 2 cut(s) 1103, 1839
BstV1I GCAGC 9 cut(s) 541, 637, 727, 1001, 1123, 1735, 1900, 2305, 2446
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 5 cut(s) 1006, 1072, 1198, 1318, 1907
BstXI CCANNNNNNTGG 1 cut(s) 1667
BstYI RGATCY 5 cut(s) 1006, 1072, 1198, 1318, 1907
BsuI GTATCC 4 cut(s) 868, 917, 1337, 2273
BsuRI GGCC 9 cut(s) 5, 88, 162, 208, 615, 1659, 2066, 2082, 2134
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 4 cut(s) 199, 705, 837, 1957
BtsI GCAGTG 2 cut(s) 630, 1467
BtsIMutI CAGTG 6 cut(s) 630, 1156, 1407, 1467, 2484, 2549
BtuMI TCGCGA 1 cut(s) 1839
BveI ACCTGC 2 cut(s) 1978, 2372
Cac8I GCNNGC 7 cut(s) 7, 118, 355, 1546, 1703, 1736, 2538
CfoI GCGC 2 cut(s) 11, 1103
Cfr13I GGNCC 8 cut(s) 206, 452, 513, 525, 1657, 1778, 2064, 2507
CseI GACGC 1 cut(s) 1374
CsiI ACCWGGT 1 cut(s) 2221
Csp6I GTAC 6 cut(s) 124, 1284, 1498, 1526, 1743, 2410
CviQI GTAC 6 cut(s) 124, 1284, 1498, 1526, 1743, 2410
DdeI CTNAG 7 cut(s) 224, 385, 534, 597, 960, 1348, 1739
DpnI GATC 9 cut(s) 1008, 1047, 1074, 1119, 1200, 1245, 1320, 1844, 1909
DpnII GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
DraIII CACNNNGTG 1 cut(s) 2177
EaeI YGGCCR 2 cut(s) 160, 2080
Eam1104I CTCTTC 2 cut(s) 915, 1614
EarI CTCTTC 2 cut(s) 915, 1614
EciI GGCGGA 1 cut(s) 2037
Ecl136II GAGCTC 1 cut(s) 1548
Eco130I CCWWGG 3 cut(s) 616, 1049, 2229
Eco147I AGGCCT 1 cut(s) 2134
Eco24I GRGCYC 2 cut(s) 1550, 2036
Eco31I GGTCTC 1 cut(s) 2478
Eco32I GATATC 1 cut(s) 474
Eco47I GGWCC 5 cut(s) 452, 513, 525, 1778, 2507
Eco53kI GAGCTC 1 cut(s) 1548
Eco57I CTGAAG 3 cut(s) 493, 519, 1059
Eco88I CYCGRG 1 cut(s) 1334
EcoICRI GAGCTC 1 cut(s) 1548
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoO109I RGGNCCY 3 cut(s) 206, 452, 513
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 6 cut(s) 88, 111, 2060, 2134, 2221, 2390
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 3 cut(s) 616, 1049, 2229
EcoT38I GRGCYC 2 cut(s) 1550, 2036
ErhI CCWWGG 3 cut(s) 616, 1049, 2229
FaqI GGGAC 3 cut(s) 465, 538, 1576
FauI CCCGC 1 cut(s) 1694
FblI GTMKAC 1 cut(s) 1388
FokI GGATG 4 cut(s) 206, 712, 824, 1964
FriOI GRGCYC 2 cut(s) 1550, 2036
FspBI CTAG 4 cut(s) 117, 735, 1050, 1107
GlaI GCGC 2 cut(s) 10, 1102
GsaI CCCAGC 3 cut(s) 869, 968, 1658
GsuI CTGGAG 1 cut(s) 1599
HaeIII GGCC 9 cut(s) 5, 88, 162, 208, 615, 1659, 2066, 2082, 2134
HapII CCGG 4 cut(s) 159, 458, 881, 2269
HgaI GACGC 1 cut(s) 1374
HhaI GCGC 2 cut(s) 11, 1103
Hin6I GCGC 2 cut(s) 9, 1101
HinP1I GCGC 2 cut(s) 9, 1101
HincII GTYRAC 2 cut(s) 1389, 1820
HindII GTYRAC 2 cut(s) 1389, 1820
HinfI GANTC 9 cut(s) 605, 729, 1033, 1054, 1292, 1581, 1598, 1671, 1816
HpaII CCGG 4 cut(s) 159, 458, 881, 2269
HphI GGTGA 5 cut(s) 163, 194, 1261, 1837, 2235
Hpy166II GTNNAC 5 cut(s) 1234, 1286, 1389, 1820, 1976
Hpy188I TCNGA 6 cut(s) 147, 471, 478, 1374, 2432, 2576
Hpy8I GTNNAC 5 cut(s) 1234, 1286, 1389, 1820, 1976
Hpy99I CGWCG 3 cut(s) 19, 32, 1390
HpyAV CCTTC 8 cut(s) 494, 615, 886, 1212, 1297, 2109, 2200, 2520
HpyCH4III ACNGT 7 cut(s) 128, 646, 750, 1357, 1422, 2344, 2479
HpyCH4IV ACGT 5 cut(s) 14, 487, 1183, 2173, 2264
HpyF3I CTNAG 7 cut(s) 224, 385, 534, 597, 960, 1348, 1739
HpySE526I ACGT 5 cut(s) 14, 487, 1183, 2173, 2264
HspAI GCGC 2 cut(s) 9, 1101
Kpn2I TCCGGA 2 cut(s) 457, 2268
Kzo9I GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
LguI GCTCTTC 1 cut(s) 1614
LmnI GCTCC 7 cut(s) 350, 494, 665, 935, 1001, 1553, 2566
Lsp1109I GCAGC 9 cut(s) 541, 637, 727, 1001, 1123, 1735, 1900, 2305, 2446
LweI GCATC 7 cut(s) 273, 469, 1470, 1849, 1931, 2201, 2207
MabI ACCWGGT 1 cut(s) 2221
MaeI CTAG 4 cut(s) 117, 735, 1050, 1107
MaeII ACGT 5 cut(s) 14, 487, 1183, 2173, 2264
MaeIII GTNAC 3 cut(s) 895, 1351, 2464
MalI GATC 9 cut(s) 1008, 1047, 1074, 1119, 1200, 1245, 1320, 1844, 1909
MboI GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
MboII GAAGA 9 cut(s) 373, 398, 499, 902, 905, 1052, 1208, 1601, 1922
MflI RGATCY 5 cut(s) 1006, 1072, 1198, 1318, 1907
MhlI GDGCHC 3 cut(s) 41, 1550, 2036
MluCI AATT 6 cut(s) 154, 276, 1191, 1329, 1803, 2120
MlyI GAGTC 5 cut(s) 614, 738, 1590, 1665, 1825
MmeI TCCRAC 5 cut(s) 901, 979, 994, 1060, 1243
MroI TCCGGA 2 cut(s) 457, 2268
MroXI GAANNNNTTC 2 cut(s) 1270, 1833
MseI TTAA 1 cut(s) 2529
MslI CAYNNNNRTG 1 cut(s) 2330
MspA1I CMGCKG 4 cut(s) 964, 992, 1718, 2504
MspI CCGG 4 cut(s) 159, 458, 881, 2269
MspR9I CCNGG 7 cut(s) 90, 113, 882, 2062, 2136, 2223, 2392
MvaI CCWGG 6 cut(s) 90, 113, 2062, 2136, 2223, 2392
MvnI CGCG 2 cut(s) 1103, 1839
NciI CCSGG 1 cut(s) 882
NdeII GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
NheI GCTAGC 1 cut(s) 116
NlaIV GGNNCC 9 cut(s) 40, 207, 453, 515, 526, 1320, 2227, 2389, 2562
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 1351
NruI TCGCGA 1 cut(s) 1839
NspI RCATGY 3 cut(s) 244, 639, 1738
NspV TTCGAA 1 cut(s) 274
PaeI GCATGC 1 cut(s) 1738
PaeR7I CTCGAG 1 cut(s) 1334
PaqCI CACCTGC 1 cut(s) 2372
PceI AGGCCT 1 cut(s) 2134
PciSI GCTCTTC 1 cut(s) 1614
PdmI GAANNNNTTC 2 cut(s) 1270, 1833
PfeI GAWTC 4 cut(s) 1033, 1054, 1292, 1598
PleI GAGTC 5 cut(s) 613, 737, 1589, 1665, 1824
PpsI GAGTC 5 cut(s) 613, 737, 1589, 1665, 1824
Ppu21I YACGTR 1 cut(s) 488
PpuMI RGGWCCY 2 cut(s) 452, 513
Psp124BI GAGCTC 1 cut(s) 1550
Psp1406I AACGTT 1 cut(s) 1183
Psp5II RGGWCCY 2 cut(s) 452, 513
Psp6I CCWGG 6 cut(s) 88, 111, 2060, 2134, 2221, 2390
PspFI CCCAGC 3 cut(s) 865, 964, 1654
PspGI CCWGG 6 cut(s) 88, 111, 2060, 2134, 2221, 2390
PspN4I GGNNCC 9 cut(s) 40, 207, 453, 515, 526, 1320, 2227, 2389, 2562
PspPI GGNCC 8 cut(s) 206, 452, 513, 525, 1657, 1778, 2064, 2507
PspPPI RGGWCCY 2 cut(s) 452, 513
PstI CTGCAG 1 cut(s) 2464
PsuI RGATCY 5 cut(s) 1006, 1072, 1198, 1318, 1907
PvuII CAGCTG 4 cut(s) 964, 992, 1718, 2504
RruI TCGCGA 1 cut(s) 1839
RsaI GTAC 6 cut(s) 125, 1285, 1499, 1527, 1744, 2411
RsaNI GTAC 6 cut(s) 124, 1284, 1498, 1526, 1743, 2410
RseI CAYNNNNRTG 1 cut(s) 2330
SacI GAGCTC 1 cut(s) 1550
SalI GTCGAC 1 cut(s) 1387
SapI GCTCTTC 1 cut(s) 1614
SaqAI TTAA 1 cut(s) 2529
Sau3AI GATC 9 cut(s) 1006, 1045, 1072, 1117, 1198, 1243, 1318, 1842, 1907
Sau96I GGNCC 8 cut(s) 206, 452, 513, 525, 1657, 1778, 2064, 2507
ScaI AGTACT 2 cut(s) 1499, 1744
SchI GAGTC 5 cut(s) 614, 738, 1590, 1665, 1825
ScrFI CCNGG 7 cut(s) 90, 113, 882, 2062, 2136, 2223, 2392
SduI GDGCHC 3 cut(s) 41, 1550, 2036
SexAI ACCWGGT 1 cut(s) 2221
SfaNI GCATC 7 cut(s) 273, 469, 1470, 1849, 1931, 2201, 2207
SfcI CTRYAG 6 cut(s) 138, 645, 1311, 1719, 2247, 2460
Sfr274I CTCGAG 1 cut(s) 1334
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 5 cut(s) 452, 513, 525, 1778, 2507
SlaI CTCGAG 1 cut(s) 1334
SmiMI CAYNNNNRTG 1 cut(s) 2330
SmlI CTYRAG 2 cut(s) 1334, 2091
SmoI CTYRAG 2 cut(s) 1334, 2091
SphI GCATGC 1 cut(s) 1738
Sse9I AATT 6 cut(s) 154, 276, 1191, 1329, 1803, 2120
SseBI AGGCCT 1 cut(s) 2134
SsiI CCGC 4 cut(s) 557, 1103, 1701, 2022
SspMI CTAG 4 cut(s) 117, 735, 1050, 1107
SstI GAGCTC 1 cut(s) 1550
StuI AGGCCT 1 cut(s) 2134
StyD4I CCNGG 7 cut(s) 88, 111, 880, 2060, 2134, 2221, 2390
StyI CCWWGG 3 cut(s) 616, 1049, 2229
TaaI ACNGT 7 cut(s) 128, 646, 750, 1357, 1422, 2344, 2479
TaiI ACGT 5 cut(s) 17, 490, 1186, 2176, 2267
TaqI TCGA 5 cut(s) 274, 727, 1335, 1388, 2415
TasI AATT 6 cut(s) 154, 276, 1191, 1329, 1803, 2120
TatI WGTACW 4 cut(s) 123, 1497, 1742, 2409
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 4 cut(s) 1033, 1054, 1292, 1598
Tru1I TTAA 1 cut(s) 2529
Tru9I TTAA 1 cut(s) 2529
TscAI CASTG 6 cut(s) 637, 1156, 1414, 1474, 2484, 2556
TseFI GTSAC 1 cut(s) 1351
TseI GCWGC 9 cut(s) 554, 650, 740, 989, 1136, 1748, 1913, 2293, 2459
Tsp45I GTSAC 1 cut(s) 1351
TspDTI ATGAA 4 cut(s) 905, 1201, 1245, 2507
TspRI CASTG 6 cut(s) 637, 1156, 1414, 1474, 2484, 2556
VpaK11BI GGWCC 5 cut(s) 452, 513, 525, 1778, 2507
XagI CCTNNNNNAGG 1 cut(s) 93
XapI RAATTY 2 cut(s) 154, 276
XceI RCATGY 3 cut(s) 244, 639, 1738
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 1334
XmaJI CCTAGG 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 1388
XmnI GAANNNNTTC 2 cut(s) 1270, 1833
XspI CTAG 4 cut(s) 117, 735, 1050, 1107
ZrmI AGTACT 2 cut(s) 1499, 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.