Rw2G038190

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005820
Physical Location & Seq
Forward (+)
63095094 .. 63101038
5945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G038190.1

Sequence Viewer

Length: 2409 bp
ATGCTCATGGGTGGCGATTTGGAGGATGGTAATTTGGGGCCTTACCAAGACAGGCTGAGGACATTTCCAAACATGCGTGGTAAATCTTACAACCCATTGATTTTTCGAATTTTGAGGCGAATAAATGTTCGTGTCCTTTTTGTGATTTTGCTTCTGGCACTTGGAGTGGTCTTTTACATTGGAGCAAGCACTTCTCCTATCATTGTGTTCGTCTTCTCAGTTTGTATTATCAGCTTCCTTCTGGCAATGCACCTTGCCAAATGGGTTCTTGCAAAAGATGAGGGACCTCCGGAGATGGGTCAGATATCAGATGCAATACGTGATGGAGCTGAAGGTTTCTTCAGGACCCAGTATGGGACCATCTCTAAGATGGCATTTTTACTGGCTGCGGTTATCCTTTGCATATACTTATTTCGCACTACGACTCCTCAGCAAGAGTCATCTGGCCTTGGAAGGGTAGCCACTGCATGTATTACTGTAGCTGCATTTCTTTTGGGAGCTTTCTGTTCAGGTATTGCAGGGTATGTTGGGATGTGGGTATCAGTTCGTGCCAATGTTCGAGTCTCTAGTGCTGCGAGACGGTCTGCAAGGGAGGCATTGCAGATAGCTGTTCGTGCTGGTGGTTTTTCTGCTATGATTGTCGTTGGTATGGCTGTAATCGGTATTGCCATCCTGTATTCCACATTTTATGTCTGGCTGGGAGTGGATACACCGGGTTCAATGAAGGTTACTGACTTGCCTCTTCTTCTTGTTGGATACGGCTTTGGAGCTTCTTTTGTTGCCTTATTTGCTCAGCTGGGTGGGGGAATATACACAAAAGCAGCTGATGTTGGAGCAGATCTTGTTGGAAAAGTTGAGCAGGGGATTCCGGAAGATGATCCTAGGAATCCTGCTGTGATAGCAGATCTGGTTGGAGATAATGTCGGGGATTGTGCGGCTAGAGGTGCTGATCTTTTTGAAAGTATTGCTGCTGAAATAATCAGTGCTATGATACTTGGGGGAACAATGGCTCAACGTTGTAAAATTGAAGATCCTTCTGGTTTCATCTTGTTTCCTCTGGTTGTTCACTCTTTTGATCTGGTGATTTCATCGGTTGGAATACTTTCAATCAGGGGTACACGAGATTCTGGTGCGAAGGGTTCTATAGAGGATCCAATGACAATTCTTGAGAGAGGATACTCAGTCACAGTAGTTTTAGCTGTTCTGACATTTGGTGCGTCTACCCGATGGTTGTTATACACTGAACAAGCACCGTCTGCGTGGTTTCACTTTGCCTTATGTGGATTAGTTGGCATCATCACTGCATATATTTTTGTGTGGATAACCAAGTACTATACAGACTACAAGTATGAGCCTGTACGCACATTAGCTCTTGCGAGCTCCACAGGTCATGGGACTAATATAATCGCTGGCGTCAGTTTGGGTCTGGAATCAACAGCTCTTCCTGTTCTTGTCATTAGCGTAGCTATTGTTTCAGCTTACTGGCTGGGCCAGACCTCTGGACTCGTGGACGAAAATGGTGTTCCTACTGGCGGGCTGTTTGGCACAGCTGTAGCAACAATGGGCATGCTAAGTACTGCTGCTTATGTTCTCACGATGGATATGTTTGGTCCAATAGCTGATAATGCTGGTGGAATTGTAGAGATGAGTCAGCAGCCAGAAAGTGTTCGCGAGATCACCGACCTTCTTGATGCAGTAGGAAACACTACAAAAGCTACCACCAAAGGGTTTGCCATTGGATCTGCTGCACTTGCATCTTTTCTTCTGTTTAGTGCTTATATGGATGAGGTAGCTACATTTGCCCGTGAACCTTTCAAGCAGGTTGATATTGCCATTCCAGAAGTTTTTGTTGGCGGATTGTTGGGCTCTATGCTTATTTTCTTATTTAGTGCCTGGGCGTGTTCAGCAGTTGGCCGAACTGCTCAAGAGGTTGTCAGGGAAGTAAGAAGGCAATTTATTGAGAGGCCTGGTATAATGGAATATAAGGAGAAGCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTGTTTCACCTATAGCAGTTGGTATTACTTTCCGGATATTTGGATACTATACTGGGCAGCCTCTACTTGGTGCTAAAGTTGTGGCTTCCATGCTGATGTTTGCAACAGTGTCGGGTATTCTCATGGCTCTTTTCCTGAACACGGCAGGTGGTGCCTGGGATAACGCAAAGAAGTACATTGAGACGGGGGTTCTCGGAGGCAAAGGAAGTGATTGTCATAAGGCTGCAGTTACAGGAGACACAGTGGGAGACCCATTCAAAGACACAGCTGGACCTTCACTTCATGTCCTTATTAAAATGCTTGCTACAATAACGCTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

802

Amino Acids

85.17

Weight (kDa)

5.86

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 76 - 797 1.5e-254 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2204
Acc36I ACCTGC 2 cut(s) 1810, 2204
AccB1I GGYRCC 2 cut(s) 2057, 2219
AccI GTMKAC 1 cut(s) 1220
AccII CGCG 1 cut(s) 1671
AccIII TCCGGA 3 cut(s) 289, 868, 2100
AciI CCGC 4 cut(s) 389, 935, 1533, 1854
AclI AACGTT 1 cut(s) 1015
AclWI GGATC 5 cut(s) 872, 1025, 1145, 1158, 1747
AcoI YGGCCR 1 cut(s) 1912
AcsI RAATTY 1 cut(s) 108
AcuI CTGAAG 2 cut(s) 325, 351
AcyI GRCGYC 1 cut(s) 1413
AdeI CACNNNGTG 1 cut(s) 2009
AfaI GTAC 5 cut(s) 1117, 1331, 1359, 1576, 2243
AfiI CCNNNNNNNGG 7 cut(s) 296, 354, 454, 1532, 1725, 2135, 2209
AgsI TTSAA 6 cut(s) 720, 959, 1028, 1107, 1816, 2326
AjnI CCWGG 4 cut(s) 1892, 1966, 2053, 2222
AjuI GAANNNNNNNTTGG 2 cut(s) 1833, 1865
Alw21I GWGCWC 1 cut(s) 1382
Alw26I GTCTC 5 cut(s) 568, 571, 2243, 2298, 2310
AlwI GGATC 5 cut(s) 872, 1025, 1145, 1158, 1747
Aor13HI TCCGGA 3 cut(s) 289, 868, 2100
AoxI GGCC 5 cut(s) 38, 445, 1489, 1912, 1964
ApoI RAATTY 1 cut(s) 108
Asp700I GAANNNNTTC 2 cut(s) 1102, 1665
AspA2I CCTAGG 1 cut(s) 881
AspS9I GGNCC 7 cut(s) 38, 284, 345, 357, 1489, 1610, 2339
AsuC2I CCSGG 1 cut(s) 714
AsuHPI GGTGA 3 cut(s) 1093, 1669, 2067
AsuII TTCGAA 1 cut(s) 106
AvaII GGWCC 5 cut(s) 284, 345, 357, 1610, 2339
AvrII CCTAGG 1 cut(s) 881
BamHI GGATCC 1 cut(s) 1150
BanI GGYRCC 2 cut(s) 2057, 2219
BanII GRGCYC 2 cut(s) 1382, 1868
BauI CACGAG 2 cut(s) 1119, 1505
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 1382
BbvCI CCTCAGC 2 cut(s) 56, 429
BccI CCATC 8 cut(s) 20, 289, 317, 364, 368, 677, 1221, 1591
BceAI ACGGC 2 cut(s) 775, 2226
BcgI CGANNNNNNTGC 2 cut(s) 2160, 2194
BciT130I CCWGG 4 cut(s) 1894, 1968, 2055, 2224
BciVI GTATCC 4 cut(s) 700, 749, 1169, 2105
BcnI CCSGG 1 cut(s) 714
BcoDI GTCTC 5 cut(s) 568, 571, 2243, 2298, 2310
BfaI CTAG 3 cut(s) 567, 882, 939
BfmI CTRYAG 5 cut(s) 477, 1143, 1551, 2079, 2292
BfuAI ACCTGC 2 cut(s) 1810, 2204
BfuI GTATCC 4 cut(s) 700, 749, 1169, 2105
BglII AGATCT 2 cut(s) 838, 904
BlnI CCTAGG 1 cut(s) 881
BlpI GCTNAGC 1 cut(s) 792
BmcAI AGTACT 2 cut(s) 1331, 1576
Bme1390I CCNGG 5 cut(s) 714, 1894, 1968, 2055, 2224
Bme18I GGWCC 5 cut(s) 284, 345, 357, 1610, 2339
BmgT120I GGNCC 7 cut(s) 38, 284, 345, 357, 1489, 1610, 2339
BmiI GGNNCC 8 cut(s) 39, 285, 347, 358, 1152, 2059, 2221, 2394
BmrFI CCNGG 5 cut(s) 714, 1894, 1968, 2055, 2224
BmrI ACTGGG 2 cut(s) 343, 2130
BmsI GCATC 6 cut(s) 301, 1302, 1681, 1763, 2033, 2039
BmuI ACTGGG 2 cut(s) 343, 2130
BpiI GAAGAC 1 cut(s) 205
Bpu10I CCTNAGC 2 cut(s) 56, 429
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 2 cut(s) 1187, 1908
BpuMI CCSGG 1 cut(s) 714
BsaAI YACGTR 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 1413
BsaI GGTCTC 1 cut(s) 2310
BsaJI CCNNGG 5 cut(s) 448, 881, 1893, 2061, 2223
BsaWI WCCGGW 3 cut(s) 289, 868, 2100
BsaXI ACNNNNNCTCC 6 cut(s) 409, 439, 906, 936, 2295, 2325
Bsc4I CCNNNNNNNGG 7 cut(s) 296, 354, 454, 1532, 1725, 2135, 2209
Bse1I ACTGG 5 cut(s) 349, 387, 1487, 1534, 2125
Bse3DI GCAATG 2 cut(s) 252, 596
BseAI TCCGGA 3 cut(s) 289, 868, 2100
BseBI CCWGG 4 cut(s) 1894, 1968, 2055, 2224
BseDI CCNNGG 5 cut(s) 448, 881, 1893, 2061, 2223
BseGI GGATG 4 cut(s) 31, 537, 669, 1789
BseLI CCNNNNNNNGG 7 cut(s) 296, 354, 454, 1532, 1725, 2135, 2209
BseMI GCAATG 2 cut(s) 252, 596
BseMII CTCAG 5 cut(s) 47, 231, 443, 806, 1194
BseNI ACTGG 5 cut(s) 349, 387, 1487, 1534, 2125
BseRI GAGGAG 1 cut(s) 417
BseYI CCCAGC 3 cut(s) 697, 796, 1486
BsgI GTGCAG 1 cut(s) 1731
Bsh1236I CGCG 1 cut(s) 1671
BshFI GGCC 5 cut(s) 40, 447, 1491, 1914, 1966
BshNI GGYRCC 2 cut(s) 2057, 2219
BsiHKAI GWGCWC 1 cut(s) 1382
BsiSI CCGG 4 cut(s) 290, 713, 869, 2101
BslFI GGGAC 3 cut(s) 297, 370, 1408
BslI CCNNNNNNNGG 7 cut(s) 296, 354, 454, 1532, 1725, 2135, 2209
BsmAI GTCTC 5 cut(s) 568, 571, 2243, 2298, 2310
BsmBI CGTCTC 2 cut(s) 571, 2243
BsmFI GGGAC 3 cut(s) 297, 370, 1408
BsnI GGCC 5 cut(s) 40, 447, 1491, 1914, 1966
Bso31I GGTCTC 1 cut(s) 2310
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 1382, 1868
Bsp13I TCCGGA 3 cut(s) 289, 868, 2100
Bsp143I GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp68I TCGCGA 1 cut(s) 1671
BspACI CCGC 4 cut(s) 389, 935, 1533, 1854
BspANI GGCC 5 cut(s) 40, 447, 1491, 1914, 1966
BspCNI CTCAG 5 cut(s) 48, 230, 442, 805, 1193
BspEI TCCGGA 3 cut(s) 289, 868, 2100
BspFNI CGCG 1 cut(s) 1671
BspLI GGNNCC 8 cut(s) 39, 285, 347, 358, 1152, 2059, 2221, 2394
BspMAI CTGCAG 1 cut(s) 2296
BspMI ACCTGC 2 cut(s) 1810, 2204
BspPI GGATC 5 cut(s) 872, 1025, 1145, 1158, 1747
BspQI GCTCTTC 1 cut(s) 1446
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 2 cut(s) 2057, 2219
BspTNI GGTCTC 1 cut(s) 2310
BsrDI GCAATG 2 cut(s) 252, 596
BsrI ACTGG 5 cut(s) 349, 387, 1487, 1534, 2125
BssECI CCNNGG 5 cut(s) 448, 881, 1893, 2061, 2223
BssMI GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
BssNI GRCGYC 1 cut(s) 1413
BssSI CACGAG 2 cut(s) 1119, 1505
BssT1I CCWWGG 3 cut(s) 448, 881, 2061
Bst2BI CACGAG 2 cut(s) 1119, 1505
Bst2UI CCWGG 4 cut(s) 1894, 1968, 2055, 2224
Bst4CI ACNGT 6 cut(s) 478, 582, 1189, 1254, 2176, 2311
Bst6I CTCTTC 2 cut(s) 747, 1446
BstACI GRCGYC 1 cut(s) 1413
BstAPI GCANNNNNTGC 2 cut(s) 1256, 2219
BstBAI YACGTR 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 6 cut(s) 187, 1378, 1411, 1535, 1568, 2370
BstDEI CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1180, 1571
BstF5I GGATG 4 cut(s) 31, 537, 669, 1789
BstFNI CGCG 1 cut(s) 1671
BstKTI GATC 9 cut(s) 841, 880, 907, 952, 1033, 1078, 1153, 1677, 1742
BstMAI GTCTC 5 cut(s) 568, 571, 2243, 2298, 2310
BstMBI GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
BstNI CCWGG 4 cut(s) 1894, 1968, 2055, 2224
BstNSI RCATGY 3 cut(s) 76, 471, 1570
BstSCI CCNGG 5 cut(s) 712, 1892, 1966, 2053, 2222
BstSFI CTRYAG 5 cut(s) 477, 1143, 1551, 2079, 2292
BstUI CGCG 1 cut(s) 1671
BstV2I GAAGAC 1 cut(s) 205
BstX2I RGATCY 5 cut(s) 838, 904, 1030, 1150, 1739
BstXI CCANNNNNNTGG 1 cut(s) 1499
BstYI RGATCY 5 cut(s) 838, 904, 1030, 1150, 1739
BsuI GTATCC 4 cut(s) 700, 749, 1169, 2105
BsuRI GGCC 5 cut(s) 40, 447, 1491, 1914, 1966
BtsCI GGATG 4 cut(s) 31, 537, 669, 1789
BtsI GCAGTG 2 cut(s) 462, 1299
BtsIMutI CAGTG 6 cut(s) 462, 988, 1239, 1299, 2181, 2316
BtuMI TCGCGA 1 cut(s) 1671
BveI ACCTGC 2 cut(s) 1810, 2204
Cac8I GCNNGC 6 cut(s) 187, 1378, 1411, 1535, 1568, 2370
Cfr13I GGNCC 7 cut(s) 38, 284, 345, 357, 1489, 1610, 2339
CseI GACGC 2 cut(s) 1206, 1402
CsiI ACCWGGT 1 cut(s) 2053
Csp6I GTAC 5 cut(s) 1116, 1330, 1358, 1575, 2242
CviAII CATG 9 cut(s) 7, 73, 468, 1391, 1567, 2158, 2191, 2351, 2389
CviQI GTAC 5 cut(s) 1116, 1330, 1358, 1575, 2242
DdeI CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1180, 1571
DpnI GATC 9 cut(s) 840, 879, 906, 951, 1032, 1077, 1152, 1676, 1741
DpnII GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
DraIII CACNNNGTG 1 cut(s) 2009
EaeI YGGCCR 1 cut(s) 1912
Eam1104I CTCTTC 2 cut(s) 747, 1446
EarI CTCTTC 2 cut(s) 747, 1446
EciI GGCGGA 1 cut(s) 1869
Ecl136II GAGCTC 1 cut(s) 1380
Eco130I CCWWGG 3 cut(s) 448, 881, 2061
Eco147I AGGCCT 1 cut(s) 1966
Eco24I GRGCYC 2 cut(s) 1382, 1868
Eco31I GGTCTC 1 cut(s) 2310
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 5 cut(s) 284, 345, 357, 1610, 2339
Eco53kI GAGCTC 1 cut(s) 1380
Eco57I CTGAAG 2 cut(s) 325, 351
EcoICRI GAGCTC 1 cut(s) 1380
EcoO109I RGGNCCY 3 cut(s) 38, 284, 345
EcoRII CCWGG 4 cut(s) 1892, 1966, 2053, 2222
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 3 cut(s) 448, 881, 2061
EcoT38I GRGCYC 2 cut(s) 1382, 1868
ErhI CCWWGG 3 cut(s) 448, 881, 2061
Esp3I CGTCTC 2 cut(s) 571, 2243
FaeI CATG 9 cut(s) 10, 76, 471, 1394, 1570, 2161, 2194, 2354, 2392
FaqI GGGAC 3 cut(s) 297, 370, 1408
FatI CATG 9 cut(s) 6, 72, 467, 1390, 1566, 2157, 2190, 2350, 2388
FauI CCCGC 1 cut(s) 1526
FblI GTMKAC 1 cut(s) 1220
FokI GGATG 4 cut(s) 38, 544, 656, 1796
FriOI GRGCYC 2 cut(s) 1382, 1868
FspBI CTAG 3 cut(s) 567, 882, 939
GsaI CCCAGC 3 cut(s) 701, 800, 1490
HaeIII GGCC 5 cut(s) 40, 447, 1491, 1914, 1966
HapII CCGG 4 cut(s) 290, 713, 869, 2101
HgaI GACGC 2 cut(s) 1206, 1402
Hin1I GRCGYC 1 cut(s) 1413
Hin1II CATG 9 cut(s) 10, 76, 471, 1394, 1570, 2161, 2194, 2354, 2392
HinfI GANTC 9 cut(s) 424, 437, 561, 865, 886, 1124, 1430, 1503, 1648
HpaII CCGG 4 cut(s) 290, 713, 869, 2101
HphI GGTGA 3 cut(s) 1093, 1669, 2067
Hpy166II GTNNAC 5 cut(s) 1066, 1118, 1221, 1510, 1808
Hpy188I TCNGA 5 cut(s) 303, 310, 1206, 2264, 2408
Hpy8I GTNNAC 5 cut(s) 1066, 1118, 1221, 1510, 1808
HpyAV CCTTC 9 cut(s) 248, 326, 447, 718, 1044, 1129, 1694, 1941, 2352
HpyCH4III ACNGT 6 cut(s) 478, 582, 1189, 1254, 2176, 2311
HpyCH4IV ACGT 3 cut(s) 319, 1015, 2005
HpyF3I CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1180, 1571
HpySE526I ACGT 3 cut(s) 319, 1015, 2005
Hsp92I GRCGYC 1 cut(s) 1413
Hsp92II CATG 9 cut(s) 10, 76, 471, 1394, 1570, 2161, 2194, 2354, 2392
Kpn2I TCCGGA 3 cut(s) 289, 868, 2100
Kzo9I GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
LguI GCTCTTC 1 cut(s) 1446
LmnI GCTCC 7 cut(s) 182, 326, 497, 767, 833, 1385, 2398
LweI GCATC 6 cut(s) 301, 1302, 1681, 1763, 2033, 2039
MabI ACCWGGT 1 cut(s) 2053
MaeI CTAG 3 cut(s) 567, 882, 939
MaeII ACGT 3 cut(s) 319, 1015, 2005
MaeIII GTNAC 3 cut(s) 727, 1183, 2296
MalI GATC 9 cut(s) 840, 879, 906, 951, 1032, 1077, 1152, 1676, 1741
MboI GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
MboII GAAGA 8 cut(s) 205, 331, 734, 737, 884, 1040, 1433, 1754
MflI RGATCY 5 cut(s) 838, 904, 1030, 1150, 1739
MhlI GDGCHC 2 cut(s) 1382, 1868
MluCI AATT 6 cut(s) 31, 108, 1023, 1161, 1635, 1952
MlyI GAGTC 5 cut(s) 418, 446, 570, 1497, 1657
MmeI TCCRAC 5 cut(s) 733, 811, 826, 892, 1075
MroI TCCGGA 3 cut(s) 289, 868, 2100
MroXI GAANNNNTTC 2 cut(s) 1102, 1665
MseI TTAA 1 cut(s) 2361
MslI CAYNNNNRTG 1 cut(s) 2162
MspA1I CMGCKG 4 cut(s) 796, 824, 1550, 2336
MspI CCGG 4 cut(s) 290, 713, 869, 2101
MspR9I CCNGG 5 cut(s) 714, 1894, 1968, 2055, 2224
MvaI CCWGG 4 cut(s) 1894, 1968, 2055, 2224
MvnI CGCG 1 cut(s) 1671
NciI CCSGG 1 cut(s) 714
NdeII GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
NlaIII CATG 9 cut(s) 10, 76, 471, 1394, 1570, 2161, 2194, 2354, 2392
NlaIV GGNNCC 8 cut(s) 39, 285, 347, 358, 1152, 2059, 2221, 2394
NmuCI GTSAC 1 cut(s) 1183
NruI TCGCGA 1 cut(s) 1671
NspI RCATGY 3 cut(s) 76, 471, 1570
NspV TTCGAA 1 cut(s) 106
PaeI GCATGC 1 cut(s) 1570
PaqCI CACCTGC 1 cut(s) 2204
PceI AGGCCT 1 cut(s) 1966
PciSI GCTCTTC 1 cut(s) 1446
PdmI GAANNNNTTC 2 cut(s) 1102, 1665
PfeI GAWTC 4 cut(s) 865, 886, 1124, 1430
PleI GAGTC 5 cut(s) 418, 445, 569, 1497, 1656
PpsI GAGTC 5 cut(s) 418, 445, 569, 1497, 1656
Ppu21I YACGTR 1 cut(s) 320
PpuMI RGGWCCY 2 cut(s) 284, 345
Psp124BI GAGCTC 1 cut(s) 1382
Psp1406I AACGTT 1 cut(s) 1015
Psp5II RGGWCCY 2 cut(s) 284, 345
Psp6I CCWGG 4 cut(s) 1892, 1966, 2053, 2222
PspFI CCCAGC 3 cut(s) 697, 796, 1486
PspGI CCWGG 4 cut(s) 1892, 1966, 2053, 2222
PspN4I GGNNCC 8 cut(s) 39, 285, 347, 358, 1152, 2059, 2221, 2394
PspPI GGNCC 7 cut(s) 38, 284, 345, 357, 1489, 1610, 2339
PspPPI RGGWCCY 2 cut(s) 284, 345
PstI CTGCAG 1 cut(s) 2296
PsuI RGATCY 5 cut(s) 838, 904, 1030, 1150, 1739
PvuII CAGCTG 4 cut(s) 796, 824, 1550, 2336
RruI TCGCGA 1 cut(s) 1671
RsaI GTAC 5 cut(s) 1117, 1331, 1359, 1576, 2243
RsaNI GTAC 5 cut(s) 1116, 1330, 1358, 1575, 2242
RseI CAYNNNNRTG 1 cut(s) 2162
SacI GAGCTC 1 cut(s) 1382
SapI GCTCTTC 1 cut(s) 1446
SaqAI TTAA 1 cut(s) 2361
Sau3AI GATC 9 cut(s) 838, 877, 904, 949, 1030, 1075, 1150, 1674, 1739
Sau96I GGNCC 7 cut(s) 38, 284, 345, 357, 1489, 1610, 2339
ScaI AGTACT 2 cut(s) 1331, 1576
SchI GAGTC 5 cut(s) 418, 446, 570, 1497, 1657
ScrFI CCNGG 5 cut(s) 714, 1894, 1968, 2055, 2224
SduI GDGCHC 2 cut(s) 1382, 1868
SexAI ACCWGGT 1 cut(s) 2053
SfaNI GCATC 6 cut(s) 301, 1302, 1681, 1763, 2033, 2039
SfcI CTRYAG 5 cut(s) 477, 1143, 1551, 2079, 2292
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 5 cut(s) 284, 345, 357, 1610, 2339
SmiMI CAYNNNNRTG 1 cut(s) 2162
SmlI CTYRAG 2 cut(s) 1166, 1923
SmoI CTYRAG 2 cut(s) 1166, 1923
SphI GCATGC 1 cut(s) 1570
Sse9I AATT 6 cut(s) 31, 108, 1023, 1161, 1635, 1952
SseBI AGGCCT 1 cut(s) 1966
SsiI CCGC 4 cut(s) 389, 935, 1533, 1854
SspMI CTAG 3 cut(s) 567, 882, 939
SstI GAGCTC 1 cut(s) 1382
StuI AGGCCT 1 cut(s) 1966
StyD4I CCNGG 5 cut(s) 712, 1892, 1966, 2053, 2222
StyI CCWWGG 3 cut(s) 448, 881, 2061
TaaI ACNGT 6 cut(s) 478, 582, 1189, 1254, 2176, 2311
TaiI ACGT 3 cut(s) 322, 1018, 2008
TaqI TCGA 2 cut(s) 106, 559
TasI AATT 6 cut(s) 31, 108, 1023, 1161, 1635, 1952
TatI WGTACW 3 cut(s) 1329, 1574, 2241
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 4 cut(s) 865, 886, 1124, 1430
Tru1I TTAA 1 cut(s) 2361
Tru9I TTAA 1 cut(s) 2361
TscAI CASTG 6 cut(s) 469, 988, 1246, 1306, 2181, 2316
TseFI GTSAC 1 cut(s) 1183
Tsp45I GTSAC 1 cut(s) 1183
TspDTI ATGAA 4 cut(s) 737, 1033, 1077, 2339
TspRI CASTG 6 cut(s) 469, 988, 1246, 1306, 2181, 2316
VpaK11BI GGWCC 5 cut(s) 284, 345, 357, 1610, 2339
XapI RAATTY 1 cut(s) 108
XceI RCATGY 3 cut(s) 76, 471, 1570
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmaJI CCTAGG 1 cut(s) 881
XmiI GTMKAC 1 cut(s) 1220
XmnI GAANNNNTTC 2 cut(s) 1102, 1665
XspI CTAG 3 cut(s) 567, 882, 939
ZrmI AGTACT 2 cut(s) 1331, 1576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.