Rroxscaffold_2G00098440

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005820
Physical Location & Seq
Reverse (-)
19614161 .. 19621164
7004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098440.1

Sequence Viewer

Length: 2415 bp
ATGCTCATGGGTGGCGATTTGGAGGATGGTAATTTGGGGCCTTACCAAGACAGGCTGAGGACATTTCCAAACATGCGTGGTAAATCTTACAACCCATTGATTTTTCGAATTTTGACGCGAATAAATGTTCGCGTCCTTTTCGTGATTTTGCTTCTGGCACTTGGAGTGGTCTTTTACATTGGAGCAAGCACTTCTCCTATCATTGTGTTCGTCTTCTCAGTTTGTATTATCAGCTTCCTTCTGGCAATGCACCTTGCCAAATGGGTTCTTGCAAAAGATGAGGGACCTCCGGAGATGGGTCAGATATCAGATGCAATACGTGATGGAGCTGAAGGTTTCTTCAGGACCCAGTATGGGACCATCTCTAAGATGGCATTTTTACTGGCTGGGGTTATCCTTTGCATATACTTATTTCGCACTACGACTCCTCAGCAAGAGTCATCTGGCCTTGGAAGGGTAGCCACTGCATGTATTACTGTAGCTGCATTTCTTTTGGGAGCTTTCTGTTCAGGTATTGCGGGGTATGTTGGGATGTGGGTATCAGTTCGTGCCAATGTTCGAGTCTCTAGTGCTGCCAGACGGTCTGCAAGGGAGGCATTGCAGATAGCTGTTCGTGCTGGTGGTTTTTCTGCTATGATTGTTGTTGGCATGGCTGTAATCGGTATTGCCATCCTGTATTCCACATTTTATGTCTGGCTGGGAGTGGATACACCGGGTTCAATGAAGGTTACTGACTTGCCTCTTCTTCTTGTTGGATACGGCTTTGGAGCTTCTTTTGTTGCCTTATTTGCTCAGCTGGGTGGGGGAATATACACAAAAGCAGCTGATGTTGGAGCAGATCTTGTTGGAAAAGTTGAGCAGGGGATTCCTGAAGATGATCCTAGGAATCCTGCTGTGATAGCAGATCTGGTTGGAGATAATGTCGGGGATTGCGCGGCTAGAGGTGCTGATCTTTTTGAAAGTATTGCTGCTGAAATAATCAGTGCTATGATACTTGGAGGAACAATGGCTCAACGTTATCCTTCTGGTTTCATCTTGTTTCCTCTGGTTGTTCACTCTTTTGATCTGGTGATTTCATCGGTTGGAATACTCTCAATCAGGGGTACACGAGATTCCGGTGCGAAGGGTTCTGTGGAGGATCCAATGACAATTCTCGAGAGAGGATACTCAGTCACAGTAGTTTTAGCTGTTCTGACATTTGGTGCGTCGACCCGATGGTTGTTATACACTGAACAAGCACCGTCTGCGTGGTTTCACTTTGCCTTGTGTGGATTAGTTGGCATCATCACTGCATATATTTTTGTGTGGATAACCAAGTACTATACAGACTACAAGTATGAGCCTGTACGCACATTAGCTCTTGCGAGCTCCACAGGTCATGGGACTAATATAATTGCTGGAGTCAGTTTGGGTCTGGAATCAACAGCTCTTCCTGTTCTTGTCATTAGCGTAGCTATTGTTTCAGCTTACTGGCTGGGCCAGACCTCTGGACTCGTGGATGAAAATGGTGTTCCTACTGGCGGGCTGTTTGGCACAGCTGTAGCAACAATGGGCATGCTAAGTACTGCTGCGTATGTTCTCACGATGGATATGTTTGGTCCAATAGCTGATAATGCTGGAGGAATTGTAGAGATGAGTCAGCAGAAAATTGCATCCTTACAGCCAGAAAGTGTTCGCGAGATCACCGATCTTCTTGATGCAGTAGGAAACACTACAAAAGCTACCACTAAAGGGTTTGCCATTGGATCTGCTGCACTTGCATCTTTTCTTCTGTTTAGTGCTTATATGGATGAGGTAGCTACATTTGCCCGTGAACCTTTCAAGCAGGTTGATATTGCCATTCCAGAAGTTTTTGTCGGCGGATTGTTGGGCTCTATGCTTATTTTCTTATTTAGTGCCTGGGCCTGTTCAGCAGTTGGCCGAACTGCTCAAGAGGTTGTCAGGGAAGTAAGAAGGCAATTTATTGAGCGGCCTGGTATAATGGAATATAAGGAGAAGCCAGATTATGCACGTTGTGTTGCTATTGTAGCATCTGCATCTTTGAGGGAGATGATAAAACCTGGTGCCTTGGCTATTGTTTCACCTATAGCAGTTGGTATTGCGTTCCGGATATTTGGATACTATACTGGGCAACCTCTACTTGGTGCTAAAGTTGTTGCTTCCATGCTGATGTTTGCAACAGTGTCGGGTATTCTCATGGCTCTTTTCCTGAACACAGCAGGTGGTGCCTGGGATAACGCAAAGAAGTACATTGAGACAGGGGTTCTCGGAGGCAAAGGAAGTGATTGTCATAAGGCTGCAGTTACAGGAGACACAGTGGGAGACCCATTCAAAGACACAGCTGGACCTTCACTTCATGTCCTTATTAAAATGCTTGCGACAATAACGCTGGTCATGGCTCCTGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

804

Amino Acids

85.28

Weight (kDa)

6.0

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 76 - 799 2.7e-251 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2210
Acc36I ACCTGC 2 cut(s) 1816, 2210
AccB1I GGYRCC 2 cut(s) 2063, 2225
AccBSI CCGCTC 1 cut(s) 1969
AccI GTMKAC 1 cut(s) 1208
AccII CGCG 4 cut(s) 118, 132, 935, 1677
AccIII TCCGGA 2 cut(s) 289, 2106
AciI CCGC 5 cut(s) 518, 935, 1521, 1860, 1969
AclI AACGTT 1 cut(s) 1015
AclWI GGATC 4 cut(s) 872, 1133, 1146, 1753
AcoI YGGCCR 1 cut(s) 1918
AcsI RAATTY 1 cut(s) 108
AcuI CTGAAG 3 cut(s) 325, 351, 891
AdeI CACNNNGTG 1 cut(s) 2015
AfaI GTAC 5 cut(s) 1105, 1319, 1347, 1564, 2249
AfiI CCNNNNNNNGG 6 cut(s) 296, 354, 454, 1520, 1731, 2141
AgsI TTSAA 4 cut(s) 720, 959, 1822, 2332
AjnI CCWGG 4 cut(s) 1898, 1972, 2059, 2228
Alw21I GWGCWC 1 cut(s) 1370
Alw26I GTCTC 4 cut(s) 568, 2249, 2304, 2316
AlwI GGATC 4 cut(s) 872, 1133, 1146, 1753
Ama87I CYCGRG 1 cut(s) 1154
Aor13HI TCCGGA 2 cut(s) 289, 2106
AoxI GGCC 6 cut(s) 38, 445, 1477, 1902, 1918, 1970
ApeKI GCWGC 7 cut(s) 482, 572, 821, 968, 1568, 1751, 2297
ApoI RAATTY 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 1671
AspA2I CCTAGG 1 cut(s) 881
AspLEI GCGC 1 cut(s) 935
AspS9I GGNCC 8 cut(s) 38, 284, 345, 357, 1477, 1598, 1902, 2345
AsuC2I CCSGG 1 cut(s) 714
AsuHPI GGTGA 3 cut(s) 1081, 1675, 2073
AsuII TTCGAA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 5 cut(s) 284, 345, 357, 1598, 2345
AvrII CCTAGG 1 cut(s) 881
BamHI GGATCC 1 cut(s) 1138
BanI GGYRCC 2 cut(s) 2063, 2225
BanII GRGCYC 2 cut(s) 1370, 1874
BauI CACGAG 2 cut(s) 1107, 1493
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 1370
BbvCI CCTCAGC 2 cut(s) 56, 429
BbvI GCAGC 7 cut(s) 469, 559, 833, 955, 1555, 1738, 2284
BccI CCATC 8 cut(s) 20, 289, 317, 364, 368, 677, 1209, 1579
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2166, 2200
BciT130I CCWGG 4 cut(s) 1900, 1974, 2061, 2230
BciVI GTATCC 4 cut(s) 700, 749, 1157, 2111
BcnI CCSGG 1 cut(s) 714
BcoDI GTCTC 4 cut(s) 568, 2249, 2304, 2316
BfaI CTAG 3 cut(s) 567, 882, 939
BfmI CTRYAG 4 cut(s) 477, 1539, 2085, 2298
BfuAI ACCTGC 2 cut(s) 1816, 2210
BfuI GTATCC 4 cut(s) 700, 749, 1157, 2111
BglII AGATCT 2 cut(s) 838, 904
BisI GCNGC 9 cut(s) 483, 573, 822, 936, 969, 1569, 1752, 1970, 2298
BlnI CCTAGG 1 cut(s) 881
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 9 cut(s) 484, 574, 823, 937, 970, 1570, 1753, 1971, 2299
BmcAI AGTACT 2 cut(s) 1319, 1564
Bme1390I CCNGG 5 cut(s) 714, 1900, 1974, 2061, 2230
Bme18I GGWCC 5 cut(s) 284, 345, 357, 1598, 2345
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 8 cut(s) 38, 284, 345, 357, 1477, 1598, 1902, 2345
BmiI GGNNCC 8 cut(s) 39, 285, 347, 358, 1140, 2065, 2227, 2400
BmrFI CCNGG 5 cut(s) 714, 1900, 1974, 2061, 2230
BmrI ACTGGG 2 cut(s) 343, 2136
BmsI GCATC 7 cut(s) 301, 1290, 1661, 1687, 1769, 2039, 2045
BmuI ACTGGG 2 cut(s) 343, 2136
BpiI GAAGAC 1 cut(s) 205
BpmI CTGGAG 2 cut(s) 1419, 1638
Bpu10I CCTNAGC 2 cut(s) 56, 429
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 1914
BpuMI CCSGG 1 cut(s) 714
BsaAI YACGTR 1 cut(s) 320
BsaI GGTCTC 1 cut(s) 2316
BsaJI CCNNGG 5 cut(s) 448, 881, 1899, 2067, 2229
BsaWI WCCGGW 3 cut(s) 289, 1115, 2106
BsaXI ACNNNNNCTCC 8 cut(s) 409, 439, 906, 936, 1611, 1641, 2301, 2331
Bsc4I CCNNNNNNNGG 6 cut(s) 296, 354, 454, 1520, 1731, 2141
Bse1I ACTGG 5 cut(s) 349, 387, 1475, 1522, 2131
Bse3DI GCAATG 2 cut(s) 252, 596
BseAI TCCGGA 2 cut(s) 289, 2106
BseBI CCWGG 4 cut(s) 1900, 1974, 2061, 2230
BseDI CCNNGG 5 cut(s) 448, 881, 1899, 2067, 2229
BseGI GGATG 6 cut(s) 31, 537, 669, 1504, 1652, 1795
BseLI CCNNNNNNNGG 6 cut(s) 296, 354, 454, 1520, 1731, 2141
BseMI GCAATG 2 cut(s) 252, 596
BseMII CTCAG 5 cut(s) 47, 231, 443, 806, 1182
BseNI ACTGG 5 cut(s) 349, 387, 1475, 1522, 2131
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 7 cut(s) 469, 559, 833, 955, 1555, 1738, 2284
BseYI CCCAGC 4 cut(s) 386, 697, 796, 1474
BsgI GTGCAG 1 cut(s) 1737
Bsh1236I CGCG 4 cut(s) 118, 132, 935, 1677
BshFI GGCC 6 cut(s) 40, 447, 1479, 1904, 1920, 1972
BshNI GGYRCC 2 cut(s) 2063, 2225
BsiHKAI GWGCWC 1 cut(s) 1370
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 4 cut(s) 290, 713, 1116, 2107
BslFI GGGAC 3 cut(s) 297, 370, 1396
BslI CCNNNNNNNGG 6 cut(s) 296, 354, 454, 1520, 1731, 2141
BsmAI GTCTC 4 cut(s) 568, 2249, 2304, 2316
BsmFI GGGAC 3 cut(s) 297, 370, 1396
BsnI GGCC 6 cut(s) 40, 447, 1479, 1904, 1920, 1972
Bso31I GGTCTC 1 cut(s) 2316
BsoBI CYCGRG 1 cut(s) 1154
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 1370, 1874
Bsp13I TCCGGA 2 cut(s) 289, 2106
Bsp143I GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp68I TCGCGA 1 cut(s) 1677
BspACI CCGC 5 cut(s) 518, 935, 1521, 1860, 1969
BspANI GGCC 6 cut(s) 40, 447, 1479, 1904, 1920, 1972
BspCNI CTCAG 5 cut(s) 48, 230, 442, 805, 1181
BspEI TCCGGA 2 cut(s) 289, 2106
BspFNI CGCG 4 cut(s) 118, 132, 935, 1677
BspLI GGNNCC 8 cut(s) 39, 285, 347, 358, 1140, 2065, 2227, 2400
BspMAI CTGCAG 1 cut(s) 2302
BspMI ACCTGC 2 cut(s) 1816, 2210
BspPI GGATC 4 cut(s) 872, 1133, 1146, 1753
BspQI GCTCTTC 1 cut(s) 1434
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 2 cut(s) 2063, 2225
BspTNI GGTCTC 1 cut(s) 2316
BsrBI CCGCTC 1 cut(s) 1969
BsrDI GCAATG 2 cut(s) 252, 596
BsrI ACTGG 5 cut(s) 349, 387, 1475, 1522, 2131
BssECI CCNNGG 5 cut(s) 448, 881, 1899, 2067, 2229
BssMI GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
BssSI CACGAG 2 cut(s) 1107, 1493
BssT1I CCWWGG 3 cut(s) 448, 881, 2067
Bst2BI CACGAG 2 cut(s) 1107, 1493
Bst2UI CCWGG 4 cut(s) 1900, 1974, 2061, 2230
Bst4CI ACNGT 6 cut(s) 478, 582, 1177, 1242, 2182, 2317
Bst6I CTCTTC 2 cut(s) 747, 1434
BstAPI GCANNNNNTGC 2 cut(s) 1244, 2225
BstBAI YACGTR 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 5 cut(s) 187, 1366, 1523, 1556, 2376
BstDEI CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1168, 1559
BstF5I GGATG 6 cut(s) 31, 537, 669, 1504, 1652, 1795
BstFNI CGCG 4 cut(s) 118, 132, 935, 1677
BstHHI GCGC 1 cut(s) 935
BstKTI GATC 9 cut(s) 841, 880, 907, 952, 1066, 1141, 1683, 1690, 1748
BstMAI GTCTC 4 cut(s) 568, 2249, 2304, 2316
BstMBI GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
BstNI CCWGG 4 cut(s) 1900, 1974, 2061, 2230
BstNSI RCATGY 3 cut(s) 76, 471, 1558
BstSCI CCNGG 5 cut(s) 712, 1898, 1972, 2059, 2228
BstSFI CTRYAG 4 cut(s) 477, 1539, 2085, 2298
BstUI CGCG 4 cut(s) 118, 132, 935, 1677
BstV1I GCAGC 7 cut(s) 469, 559, 833, 955, 1555, 1738, 2284
BstV2I GAAGAC 1 cut(s) 205
BstX2I RGATCY 4 cut(s) 838, 904, 1138, 1745
BstXI CCANNNNNNTGG 1 cut(s) 1487
BstYI RGATCY 4 cut(s) 838, 904, 1138, 1745
BsuI GTATCC 4 cut(s) 700, 749, 1157, 2111
BsuRI GGCC 6 cut(s) 40, 447, 1479, 1904, 1920, 1972
BtsCI GGATG 6 cut(s) 31, 537, 669, 1504, 1652, 1795
BtsI GCAGTG 2 cut(s) 462, 1287
BtsIMutI CAGTG 6 cut(s) 462, 988, 1227, 1287, 2187, 2322
BtuMI TCGCGA 1 cut(s) 1677
BveI ACCTGC 2 cut(s) 1816, 2210
Cac8I GCNNGC 5 cut(s) 187, 1366, 1523, 1556, 2376
CfoI GCGC 1 cut(s) 935
Cfr13I GGNCC 8 cut(s) 38, 284, 345, 357, 1477, 1598, 1902, 2345
CseI GACGC 3 cut(s) 121, 124, 1194
CsiI ACCWGGT 1 cut(s) 2059
Csp6I GTAC 5 cut(s) 1104, 1318, 1346, 1563, 2248
CviQI GTAC 5 cut(s) 1104, 1318, 1346, 1563, 2248
DdeI CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1168, 1559
DpnI GATC 9 cut(s) 840, 879, 906, 951, 1065, 1140, 1682, 1689, 1747
DpnII GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
DraIII CACNNNGTG 1 cut(s) 2015
EaeI YGGCCR 1 cut(s) 1918
Eam1104I CTCTTC 2 cut(s) 747, 1434
EarI CTCTTC 2 cut(s) 747, 1434
EciI GGCGGA 1 cut(s) 1875
Ecl136II GAGCTC 1 cut(s) 1368
Eco130I CCWWGG 3 cut(s) 448, 881, 2067
Eco24I GRGCYC 2 cut(s) 1370, 1874
Eco31I GGTCTC 1 cut(s) 2316
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 5 cut(s) 284, 345, 357, 1598, 2345
Eco53kI GAGCTC 1 cut(s) 1368
Eco57I CTGAAG 3 cut(s) 325, 351, 891
Eco88I CYCGRG 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 1368
EcoO109I RGGNCCY 3 cut(s) 38, 284, 345
EcoRII CCWGG 4 cut(s) 1898, 1972, 2059, 2228
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 3 cut(s) 448, 881, 2067
EcoT38I GRGCYC 2 cut(s) 1370, 1874
ErhI CCWWGG 3 cut(s) 448, 881, 2067
FaqI GGGAC 3 cut(s) 297, 370, 1396
FauI CCCGC 2 cut(s) 511, 1514
FblI GTMKAC 1 cut(s) 1208
Fnu4HI GCNGC 9 cut(s) 483, 573, 822, 936, 969, 1569, 1752, 1970, 2298
FokI GGATG 6 cut(s) 38, 544, 656, 1511, 1639, 1802
FriOI GRGCYC 2 cut(s) 1370, 1874
Fsp4HI GCNGC 9 cut(s) 483, 573, 822, 936, 969, 1569, 1752, 1970, 2298
FspBI CTAG 3 cut(s) 567, 882, 939
GlaI GCGC 1 cut(s) 934
GluI GCNGC 9 cut(s) 483, 573, 822, 936, 969, 1569, 1752, 1970, 2298
GsaI CCCAGC 4 cut(s) 390, 701, 800, 1478
GsuI CTGGAG 2 cut(s) 1419, 1638
HaeIII GGCC 6 cut(s) 40, 447, 1479, 1904, 1920, 1972
HapII CCGG 4 cut(s) 290, 713, 1116, 2107
HgaI GACGC 3 cut(s) 121, 124, 1194
HhaI GCGC 1 cut(s) 935
Hin6I GCGC 1 cut(s) 933
HinP1I GCGC 1 cut(s) 933
HincII GTYRAC 1 cut(s) 1209
HindII GTYRAC 1 cut(s) 1209
HpaII CCGG 4 cut(s) 290, 713, 1116, 2107
HphI GGTGA 3 cut(s) 1081, 1675, 2073
Hpy166II GTNNAC 4 cut(s) 1054, 1106, 1209, 1814
Hpy188I TCNGA 5 cut(s) 303, 310, 1194, 2270, 2414
Hpy8I GTNNAC 4 cut(s) 1054, 1106, 1209, 1814
Hpy99I CGWCG 1 cut(s) 1210
HpyAV CCTTC 8 cut(s) 248, 326, 447, 718, 1032, 1117, 1947, 2358
HpyCH4III ACNGT 6 cut(s) 478, 582, 1177, 1242, 2182, 2317
HpyCH4IV ACGT 3 cut(s) 319, 1015, 2011
HpyF3I CTNAG 7 cut(s) 56, 217, 366, 429, 792, 1168, 1559
HpySE526I ACGT 3 cut(s) 319, 1015, 2011
HspAI GCGC 1 cut(s) 933
Kpn2I TCCGGA 2 cut(s) 289, 2106
Kzo9I GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 7 cut(s) 182, 326, 497, 767, 833, 1373, 2404
Lsp1109I GCAGC 7 cut(s) 469, 559, 833, 955, 1555, 1738, 2284
LweI GCATC 7 cut(s) 301, 1290, 1661, 1687, 1769, 2039, 2045
MabI ACCWGGT 1 cut(s) 2059
MaeI CTAG 3 cut(s) 567, 882, 939
MaeII ACGT 3 cut(s) 319, 1015, 2011
MaeIII GTNAC 3 cut(s) 727, 1171, 2302
MalI GATC 9 cut(s) 840, 879, 906, 951, 1065, 1140, 1682, 1689, 1747
MbiI CCGCTC 1 cut(s) 1969
MboI GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
MboII GAAGA 8 cut(s) 205, 331, 734, 737, 884, 1421, 1682, 1760
MflI RGATCY 4 cut(s) 838, 904, 1138, 1745
MhlI GDGCHC 2 cut(s) 1370, 1874
MluCI AATT 7 cut(s) 31, 108, 1149, 1392, 1623, 1647, 1958
MlyI GAGTC 6 cut(s) 418, 446, 570, 1410, 1485, 1645
MmeI TCCRAC 5 cut(s) 733, 811, 826, 892, 1063
MroI TCCGGA 2 cut(s) 289, 2106
MroXI GAANNNNTTC 1 cut(s) 1671
MseI TTAA 1 cut(s) 2367
MslI CAYNNNNRTG 1 cut(s) 2168
MspA1I CMGCKG 4 cut(s) 796, 824, 1538, 2342
MspI CCGG 4 cut(s) 290, 713, 1116, 2107
MspR9I CCNGG 5 cut(s) 714, 1900, 1974, 2061, 2230
MvaI CCWGG 4 cut(s) 1900, 1974, 2061, 2230
MvnI CGCG 4 cut(s) 118, 132, 935, 1677
NciI CCSGG 1 cut(s) 714
NdeII GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
NlaIV GGNNCC 8 cut(s) 39, 285, 347, 358, 1140, 2065, 2227, 2400
NmuCI GTSAC 1 cut(s) 1171
NruI TCGCGA 1 cut(s) 1677
NspI RCATGY 3 cut(s) 76, 471, 1558
NspV TTCGAA 1 cut(s) 106
PaeI GCATGC 1 cut(s) 1558
PaeR7I CTCGAG 1 cut(s) 1154
PaqCI CACCTGC 1 cut(s) 2210
PciSI GCTCTTC 1 cut(s) 1434
PdmI GAANNNNTTC 1 cut(s) 1671
PfeI GAWTC 4 cut(s) 865, 886, 1112, 1418
PkrI GCNGC 9 cut(s) 484, 574, 823, 937, 970, 1570, 1753, 1971, 2299
PleI GAGTC 6 cut(s) 418, 445, 569, 1409, 1485, 1644
PpsI GAGTC 6 cut(s) 418, 445, 569, 1409, 1485, 1644
Ppu21I YACGTR 1 cut(s) 320
PpuMI RGGWCCY 2 cut(s) 284, 345
Psp124BI GAGCTC 1 cut(s) 1370
Psp1406I AACGTT 1 cut(s) 1015
Psp5II RGGWCCY 2 cut(s) 284, 345
Psp6I CCWGG 4 cut(s) 1898, 1972, 2059, 2228
PspFI CCCAGC 4 cut(s) 386, 697, 796, 1474
PspGI CCWGG 4 cut(s) 1898, 1972, 2059, 2228
PspN4I GGNNCC 8 cut(s) 39, 285, 347, 358, 1140, 2065, 2227, 2400
PspPI GGNCC 8 cut(s) 38, 284, 345, 357, 1477, 1598, 1902, 2345
PspPPI RGGWCCY 2 cut(s) 284, 345
PstI CTGCAG 1 cut(s) 2302
PsuI RGATCY 4 cut(s) 838, 904, 1138, 1745
PvuII CAGCTG 4 cut(s) 796, 824, 1538, 2342
RruI TCGCGA 1 cut(s) 1677
RsaI GTAC 5 cut(s) 1105, 1319, 1347, 1564, 2249
RsaNI GTAC 5 cut(s) 1104, 1318, 1346, 1563, 2248
RseI CAYNNNNRTG 1 cut(s) 2168
SacI GAGCTC 1 cut(s) 1370
SalI GTCGAC 1 cut(s) 1207
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 1 cut(s) 2367
SatI GCNGC 9 cut(s) 483, 573, 822, 936, 969, 1569, 1752, 1970, 2298
Sau3AI GATC 9 cut(s) 838, 877, 904, 949, 1063, 1138, 1680, 1687, 1745
Sau96I GGNCC 8 cut(s) 38, 284, 345, 357, 1477, 1598, 1902, 2345
ScaI AGTACT 2 cut(s) 1319, 1564
SchI GAGTC 6 cut(s) 418, 446, 570, 1410, 1485, 1645
ScrFI CCNGG 5 cut(s) 714, 1900, 1974, 2061, 2230
SduI GDGCHC 2 cut(s) 1370, 1874
SexAI ACCWGGT 1 cut(s) 2059
SfaNI GCATC 7 cut(s) 301, 1290, 1661, 1687, 1769, 2039, 2045
SfcI CTRYAG 4 cut(s) 477, 1539, 2085, 2298
Sfr274I CTCGAG 1 cut(s) 1154
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 5 cut(s) 284, 345, 357, 1598, 2345
SlaI CTCGAG 1 cut(s) 1154
SmiMI CAYNNNNRTG 1 cut(s) 2168
SmlI CTYRAG 2 cut(s) 1154, 1929
SmoI CTYRAG 2 cut(s) 1154, 1929
SphI GCATGC 1 cut(s) 1558
Sse9I AATT 7 cut(s) 31, 108, 1149, 1392, 1623, 1647, 1958
SsiI CCGC 5 cut(s) 518, 935, 1521, 1860, 1969
SspMI CTAG 3 cut(s) 567, 882, 939
SstI GAGCTC 1 cut(s) 1370
StyD4I CCNGG 5 cut(s) 712, 1898, 1972, 2059, 2228
StyI CCWWGG 3 cut(s) 448, 881, 2067
TaaI ACNGT 6 cut(s) 478, 582, 1177, 1242, 2182, 2317
TaiI ACGT 3 cut(s) 322, 1018, 2014
TaqI TCGA 4 cut(s) 106, 559, 1155, 1208
TasI AATT 7 cut(s) 31, 108, 1149, 1392, 1623, 1647, 1958
TatI WGTACW 3 cut(s) 1317, 1562, 2247
TauI GCSGC 2 cut(s) 938, 1972
TfiI GAWTC 4 cut(s) 865, 886, 1112, 1418
Tru1I TTAA 1 cut(s) 2367
Tru9I TTAA 1 cut(s) 2367
TscAI CASTG 6 cut(s) 469, 988, 1234, 1294, 2187, 2322
TseFI GTSAC 1 cut(s) 1171
TseI GCWGC 7 cut(s) 482, 572, 821, 968, 1568, 1751, 2297
Tsp45I GTSAC 1 cut(s) 1171
TspDTI ATGAA 5 cut(s) 737, 1021, 1065, 1515, 2345
TspRI CASTG 6 cut(s) 469, 988, 1234, 1294, 2187, 2322
VpaK11BI GGWCC 5 cut(s) 284, 345, 357, 1598, 2345
XapI RAATTY 1 cut(s) 108
XceI RCATGY 3 cut(s) 76, 471, 1558
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 1154
XmaJI CCTAGG 1 cut(s) 881
XmiI GTMKAC 1 cut(s) 1208
XmnI GAANNNNTTC 1 cut(s) 1671
XspI CTAG 3 cut(s) 567, 882, 939
ZrmI AGTACT 2 cut(s) 1319, 1564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.