FvH4_6g37430
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011195
Physical Location & Seq
Reverse (-)
29445537 .. 29450794
5258 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37430.t1

Sequence Viewer

Length: 3027 bp
ATGCAGTTCCATTTCCTGTTCTTGTTCTTGTGCTTCTCTGTTTCCATAACCACTATAACTAGTTTGAGCTCTGATGGGCTTGTTCTACTGTCCCTCTTAAACCACTGGACTTTTGTCCCCAACCCCATTTCCTCAAGTTGGAATCTTTCTGATTCCACACCATGCAATTGGGTAGGAGTAGAATGTGATCCGGCCCACAATGTTCTTGCCTTAAATGTCTCTAGTTATGACATTTCTGGTCAGTTAGGGTCTGAAATTGGGAACTTGAGTCACTTAGAGACTCTTGTTTTGAGTTCCAATAATTTTTCTGGCCAAATTCCCATGGAGTTGGCCGATTGTAGTCTTCTTGAGACCTTGGACTTGTCTGGAAATGGGTTCTCTGGAAAAGTTCCTGATAACTTGGACAAGTTGGGAAATTTACAGTACTTGAGTTTGAGTGATAATTATCTGGAAGGGCAGATTCCTTCATCTATTGGAAATTGCAAGAACTTGGTTTACTTGGATTTGTCATCCAATCAGTTTAGTGGAGGTGTTCCTTCTGCACTTGGCAATTGTACTGGTTTAGAGGAATTTTCAGCTGCAGTTAACAAATTAGTTGGTCCAATACCTTCTTCCTTTGGCCTACTAGATAAGCTACGAATTCTATACCTTCCTGAGAATCAGCTGTCAGGAAGAATACCTCCAGAACTTGGTAAATGCAAGTCCTTGACCGAACTACAATTGTATTCGAACCAGCTAGTTGGAGAAATTCCTGATGAGTTGGGAATGTTGACCAACCTGCAGGACCTTGAATTATTCCAAAACAACTTAACTGGTGAGATTCCAGTTAGCATATGGAAGATTCAAACTCTTCAATATGTCCATCTCTACATGAATAACCTCACTGGGGAACTGCCTTTAGAGATGACGGAGCTCAAGCAACTTAAAAACATCACATTGTACAACAACCAGTTCTATGGAGTTGTACCTCAAGCTTTCGGGATTAACAGCAGTTTAGTTTGGGTGGATTTTATGTATAATAAGTTCACTGGTAGCATCCCTCCAAATCTTTGCTTTGGAAAGCAGCTATGGGCATTGAATATGGGGTTCAATCAATTTCAAGGTACCATACCTTCTGATGTCGGAAGTTGTTCAACTCTTTGGAGGATGAGACTTGAACAGAATAACCTGACCGGAGTTCTCCCACAATTTGTGAAAAATTCTGAGCTTTCATATATGGACATCAGCAGCAATGAGATTAGTGGAGAAATTCCATCAAGCTTGGGAAACTGTAGCAGCCTCACCACCATCATCTTGTCCATGAACAACTTAACAGGAGTTATACCAGAAGAGCTTGGTTACCTTGCAGATCTTCAAAAGTTGAATCTTTCCCACAATAATTTGGTGGGTGCTTTGCCTTCTAGCCTATCCAATTGTACCAAAATGCAGGAGTTTGACGTGGGGTCCAACTTCTTGAATGGTTCAATTCCTTTGAGTTTGAGCAGATGGACAGGTTTATCTAAATTGGTATTGAGTGAAAACAGGTTCACTGGCGGCATTCCACCTTTCTTGTCAGAATTTGAAAAGCTTTCCGAGCTACAACTTGGTGGAAATTTGTTGGGAGGTGTGATTCCATCGTCGGTTGGAGCTCTGCAGAATTTGTTTTATGCTATGAACCTTAGCAATAATGCATTGACAGGTGAGGTCCCTTCAGACATTGGAAAGCTGATGATGCTACAGCGGTTAGATTTATCTCATAACAACTTGACAGGGAGTTTAAGAGTTCTTGATGGCATGAAGTCATTAATTGAAGTTAATGTTTCATTCAATGGCTTCACTGGTGCAGTACCAGAAACACTGATGAACCTGTTGAACTCATCCTCATTTTCCTTTTCCAGCAATCCCTCCCTATGTGTCAGCTACCTTCCATCATGTGGCTACACTTGTGCCGGAAACAACACTATTAAACTCTGTAATAGTCCATCAAGCAAGCCGAAAGGACTTAGCAAAGTGGGAATTGCATTTATAGCGGTCGGATCATCGATACTTGTTGTTTCTCTGCTGTATCTACTGCTTTATTTGTTTTGCTTGAGCAGAAGGACTAAGAAGGAGCTTGGGGTCTCTGATCGAGAGGGTCCATCTTCCTTACTCACAAAAGTCATGGAGGCTACAGAGAACCTTAATGATAGATATATTATTGGGAGAGGAGCCCATGGAACTGTTTATAGAGCTTCTTTGGATGATGATGAAGATTTTGCTGTCAAGAAGCTTGTATTTGCAGGGCATGAAGGAACGCGTTTGAGCATGATAAGAGAACTTCAAACCCTTGGGAAGATCAAGCACCGGAATCTGGTCAAATTAGAAGACTTCTGGTTAAGAAAGGACTATGGCTTAATCTTGTACAGGTACATGCAGAACGGGAGCCTTCATGATGTTTTACATGAAATTAGTCCGCCACTAATACTGGAGTGGAGTGTCCGCTACAAGATAGCACTGGGAACTGCATACGCTTTGGAATATCTCCATTATGATTGTGTGCCCCCAATAGTGCATCGTGATATCAAGCCAATGAACATTCTCTTAGACTCTGAGATGGAAGCTCATATTTCTGATTTTGGTATTGCTAAACTCTTGGATCAGTCCACTCCTTCAACGATTTCCATCTCAGTTGTTGGTACAACTGGATATATCGCACCAGAAAATGCATTCAGAACAGCAAACAGTGTGGAGTCTGATGTGTACGGTTATGGGGTTGTATTACTTGAGCTGATAACTAGAAGGAAGGCATTGGATCCATCTTTTATGGAGCAAACTGACATTGTAGGATGGGTTAGATCAGTGTGGAGTGATACAGAAAAAATTGATAGGATTGTTGATTCAAGCATTATGGAGGAACTTCTAGACTCAAGTATCAGGGACCAGATTGTTGATGTATTTTTGGTGGCTCTCAGCTGTATTCATAATGATCCTAAAAAGCGACCCATGATGAGAGATGTTATCAAGCAATTATTAGATGTAAAGTCCCCAATGAGGAGCACAAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1009

Amino Acids

111.1

Weight (kDa)

5.12

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 63 3.9e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 68 - 193 4.6e-09 Leucine-rich repeat region
LRR_8 PF13855 402 - 461 5.8e-08 Leucine rich repeat
LRR_8 PF13855 522 - 582 9.9e-06 Leucine rich repeat
Pkinase PF00069 725 - 994 3.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 725 - 996 1.2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 786
Acc65I GGTACC 1 cut(s) 1103
AccB1I GGYRCC 1 cut(s) 1103
AccB7I CCANNNNNTGG 2 cut(s) 689, 1913
AccII CGCG 1 cut(s) 2275
AciI CCGC 5 cut(s) 1533, 1720, 2009, 2432, 2458
AclWI GGATC 6 cut(s) 182, 2023, 2622, 2765, 2778, 2939
AcoI YGGCCR 2 cut(s) 310, 330
AcuI CTGAAG 1 cut(s) 1674
AfiI CCNNNNNNNGG 6 cut(s) 138, 689, 886, 1913, 2329, 3009
AflIII ACRYGT 1 cut(s) 2273
AhdI GACNNNNNGTC 1 cut(s) 1441
AhlI ACTAGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 595, 627
Alw21I GWGCWC 4 cut(s) 71, 915, 1630, 3017
Alw26I GTCTC 5 cut(s) 223, 272, 344, 1144, 2104
AlwI GGATC 6 cut(s) 182, 2023, 2622, 2765, 2778, 2939
AoxI GGCC 4 cut(s) 192, 310, 330, 619
ApeKI GCWGC 4 cut(s) 578, 1063, 1227, 1275
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
AseI ATTAAT 1 cut(s) 1784
Asp700I GAANNNNTTC 1 cut(s) 1129
Asp718I GGTACC 1 cut(s) 1103
AspS9I GGNCC 7 cut(s) 193, 599, 784, 1443, 1684, 2114, 2896
AsuHPI GGTGA 3 cut(s) 827, 1273, 1691
AsuII TTCGAA 1 cut(s) 728
AvaII GGWCC 6 cut(s) 599, 784, 1443, 1684, 2114, 2896
BaeGI GKGCMC 1 cut(s) 2520
BaeI ACNNNNGTAYC 2 cut(s) 2872, 2905
BalI TGGCCA 1 cut(s) 312
BamHI GGATCC 1 cut(s) 2770
BanI GGYRCC 1 cut(s) 1103
BanII GRGCYC 4 cut(s) 71, 915, 1630, 2191
BarI GAAGNNNNNNTAC 2 cut(s) 1096, 1128
BbsI GAAGAC 2 cut(s) 335, 2349
Bbv12I GWGCWC 4 cut(s) 71, 915, 1630, 3017
BbvI GCAGC 4 cut(s) 565, 1075, 1239, 1287
BcoDI GTCTC 5 cut(s) 223, 272, 344, 1144, 2104
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 8 cut(s) 60, 222, 626, 737, 1401, 2754, 2879, 3025
BfmI CTRYAG 6 cut(s) 579, 779, 1270, 1631, 1715, 2148
BfuAI ACCTGC 1 cut(s) 786
BglII AGATCT 1 cut(s) 1348
BisI GCNGC 5 cut(s) 579, 1064, 1228, 1276, 1534
BlsI GCNGC 5 cut(s) 580, 1065, 1229, 1277, 1535
BmcAI AGTACT 1 cut(s) 425
Bme18I GGWCC 6 cut(s) 599, 784, 1443, 1684, 2114, 2896
BmeRI GACNNNNNGTC 1 cut(s) 1441
BmgBI CACGTC 1 cut(s) 1438
BmgT120I GGNCC 7 cut(s) 193, 599, 784, 1443, 1684, 2114, 2896
BmiI GGNNCC 8 cut(s) 1105, 1444, 1686, 2115, 2188, 2402, 2772, 2897
BmrI ACTGGG 2 cut(s) 894, 2483
BmsI GCATC 3 cut(s) 1044, 1701, 2539
BmuI ACTGGG 2 cut(s) 894, 2483
BoxI GACNNNNGTC 1 cut(s) 113
BpiI GAAGAC 2 cut(s) 335, 2349
BpmI CTGGAG 2 cut(s) 666, 2465
Bpu10I CCTNAGC 1 cut(s) 1658
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 9 cut(s) 118, 286, 368, 448, 899, 954, 2089, 2762, 2869
Bsa29I ATCGAT 1 cut(s) 2021
BsaBI GATNNNNATC 2 cut(s) 444, 2639
BsaI GGTCTC 2 cut(s) 344, 2104
BsaJI CCNNGG 4 cut(s) 321, 354, 2191, 2305
BsaWI WCCGGW 2 cut(s) 1172, 2322
Bsc4I CCNNNNNNNGG 6 cut(s) 138, 689, 886, 1913, 2329, 3009
Bse3DI GCAATG 1 cut(s) 1237
Bse8I GATNNNNATC 2 cut(s) 444, 2639
BseCI ATCGAT 1 cut(s) 2021
BseDI CCNNGG 4 cut(s) 321, 354, 2191, 2305
BseGI GGATG 6 cut(s) 509, 1035, 1152, 1856, 2224, 2810
BseJI GATNNNNATC 2 cut(s) 444, 2639
BseLI CCNNNNNNNGG 6 cut(s) 138, 689, 886, 1913, 2329, 3009
BseMI GCAATG 1 cut(s) 1237
BseMII CTCAG 5 cut(s) 645, 1194, 2559, 2658, 2941
BseRI GAGGAG 2 cut(s) 2199, 3025
BseSI GKGCMC 1 cut(s) 2520
BseXI GCAGC 4 cut(s) 565, 1075, 1239, 1287
BsgI GTGCAG 2 cut(s) 525, 1842
Bsh1236I CGCG 1 cut(s) 2275
Bsh1285I CGRYCG 1 cut(s) 2013
BshFI GGCC 4 cut(s) 194, 312, 332, 621
BshNI GGYRCC 1 cut(s) 1103
BshVI ATCGAT 1 cut(s) 2021
BsiEI CGRYCG 1 cut(s) 2013
BsiHKAI GWGCWC 4 cut(s) 71, 915, 1630, 3017
BsiSI CCGG 4 cut(s) 191, 1173, 1929, 2323
BslFI GGGAC 5 cut(s) 76, 101, 1670, 2909, 2986
BslI CCNNNNNNNGG 6 cut(s) 138, 689, 886, 1913, 2329, 3009
BsmAI GTCTC 5 cut(s) 223, 272, 344, 1144, 2104
BsmFI GGGAC 5 cut(s) 76, 101, 1670, 2909, 2986
BsmI GAATGC 2 cut(s) 1536, 2684
BsnI GGCC 4 cut(s) 194, 312, 332, 621
Bso31I GGTCTC 2 cut(s) 344, 2104
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 6 cut(s) 71, 915, 1630, 2191, 2520, 3017
Bsp1407I TGTACA 2 cut(s) 939, 2379
Bsp143I GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
Bsp19I CCATGG 2 cut(s) 321, 2191
BspACI CCGC 5 cut(s) 1533, 1720, 2009, 2432, 2458
BspANI GGCC 4 cut(s) 194, 312, 332, 621
BspCNI CTCAG 5 cut(s) 646, 1195, 2560, 2657, 2940
BspDI ATCGAT 1 cut(s) 2021
BspFNI CGCG 1 cut(s) 2275
BspHI TCATGA 1 cut(s) 2407
BspLI GGNNCC 8 cut(s) 1105, 1444, 1686, 2115, 2188, 2402, 2772, 2897
BspMAI CTGCAG 3 cut(s) 583, 783, 1635
BspMI ACCTGC 1 cut(s) 786
BspPI GGATC 6 cut(s) 182, 2023, 2622, 2765, 2778, 2939
BspQI GCTCTTC 1 cut(s) 1323
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 1103
BspTNI GGTCTC 2 cut(s) 344, 2104
BsrDI GCAATG 1 cut(s) 1237
BsrGI TGTACA 2 cut(s) 939, 2379
BssECI CCNNGG 4 cut(s) 321, 354, 2191, 2305
BssMI GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
BssT1I CCWWGG 4 cut(s) 321, 354, 2191, 2305
Bst4CI ACNGT 6 cut(s) 90, 423, 1271, 2200, 2702, 2723
Bst6I CTCTTC 2 cut(s) 855, 1323
BstAUI TGTACA 2 cut(s) 939, 2379
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 1 cut(s) 1970
BstDSI CCRYGG 2 cut(s) 321, 2191
BstEII GGTNACC 1 cut(s) 1337
BstF5I GGATG 6 cut(s) 509, 1035, 1152, 1856, 2224, 2810
BstFNI CGCG 1 cut(s) 2275
BstKTI GATC 9 cut(s) 190, 1351, 2018, 2107, 2316, 2617, 2773, 2816, 2947
BstMAI GTCTC 5 cut(s) 223, 272, 344, 1144, 2104
BstMBI GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
BstMCI CGRYCG 1 cut(s) 2013
BstMWI GCNNNNNNNGC 3 cut(s) 1573, 1711, 2006
BstNSI RCATGY 1 cut(s) 2392
BstPAI GACNNNNGTC 1 cut(s) 113
BstPI GGTNACC 1 cut(s) 1337
BstSFI CTRYAG 6 cut(s) 579, 779, 1270, 1631, 1715, 2148
BstSLI GKGCMC 1 cut(s) 2520
BstUI CGCG 1 cut(s) 2275
BstV1I GCAGC 4 cut(s) 565, 1075, 1239, 1287
BstV2I GAAGAC 2 cut(s) 335, 2349
BstX2I RGATCY 2 cut(s) 1348, 2770
BstXI CCANNNNNNTGG 4 cut(s) 168, 328, 740, 956
BstYI RGATCY 2 cut(s) 1348, 2770
Bsu15I ATCGAT 1 cut(s) 2021
BsuRI GGCC 4 cut(s) 194, 312, 332, 621
BsuTUI ATCGAT 1 cut(s) 2021
BtgI CCRYGG 2 cut(s) 321, 2191
BtrI CACGTC 1 cut(s) 1438
BtsCI GGATG 6 cut(s) 509, 1035, 1152, 1856, 2224, 2810
BtsIMutI CAGTG 9 cut(s) 103, 882, 1026, 1527, 1815, 1835, 2471, 2707, 2823
BveI ACCTGC 1 cut(s) 786
Cac8I GCNNGC 1 cut(s) 1970
CciI TCATGA 1 cut(s) 2407
Cfr13I GGNCC 7 cut(s) 193, 599, 784, 1443, 1684, 2114, 2896
ClaI ATCGAT 1 cut(s) 2021
CspCI CAANNNNNGTGG 4 cut(s) 1530, 1565, 2685, 2720
DpnI GATC 9 cut(s) 189, 1350, 2017, 2106, 2315, 2616, 2772, 2815, 2946
DpnII GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
DriI GACNNNNNGTC 1 cut(s) 1441
EaeI YGGCCR 2 cut(s) 310, 330
Eam1104I CTCTTC 2 cut(s) 855, 1323
Eam1105I GACNNNNNGTC 1 cut(s) 1441
EarI CTCTTC 2 cut(s) 855, 1323
EciI GGCGGA 1 cut(s) 2421
Ecl136II GAGCTC 3 cut(s) 69, 913, 1628
Eco130I CCWWGG 4 cut(s) 321, 354, 2191, 2305
Eco24I GRGCYC 4 cut(s) 71, 915, 1630, 2191
Eco31I GGTCTC 2 cut(s) 344, 2104
Eco32I GATATC 1 cut(s) 2539
Eco47I GGWCC 6 cut(s) 599, 784, 1443, 1684, 2114, 2896
Eco53kI GAGCTC 3 cut(s) 69, 913, 1628
Eco57I CTGAAG 1 cut(s) 1674
Eco91I GGTNACC 1 cut(s) 1337
EcoICRI GAGCTC 3 cut(s) 69, 913, 1628
EcoO109I RGGNCCY 2 cut(s) 784, 1684
EcoO65I GGTNACC 1 cut(s) 1337
EcoRI GAATTC 1 cut(s) 639
EcoRV GATATC 1 cut(s) 2539
EcoT14I CCWWGG 4 cut(s) 321, 354, 2191, 2305
EcoT22I ATGCAT 2 cut(s) 1672, 2686
EcoT38I GRGCYC 4 cut(s) 71, 915, 1630, 2191
ErhI CCWWGG 4 cut(s) 321, 354, 2191, 2305
FaqI GGGAC 5 cut(s) 76, 101, 1670, 2909, 2986
FauNDI CATATG 1 cut(s) 833
Fnu4HI GCNGC 5 cut(s) 579, 1064, 1228, 1276, 1534
FokI GGATG 6 cut(s) 496, 1022, 1159, 1843, 2231, 2817
FriOI GRGCYC 4 cut(s) 71, 915, 1630, 2191
Fsp4HI GCNGC 5 cut(s) 579, 1064, 1228, 1276, 1534
FspBI CTAG 8 cut(s) 60, 222, 626, 737, 1401, 2754, 2879, 3025
GluI GCNGC 5 cut(s) 579, 1064, 1228, 1276, 1534
GsuI CTGGAG 2 cut(s) 666, 2465
HaeIII GGCC 4 cut(s) 194, 312, 332, 621
HapII CCGG 4 cut(s) 191, 1173, 1929, 2323
HincII GTYRAC 2 cut(s) 586, 771
HindII GTYRAC 2 cut(s) 586, 771
HindIII AAGCTT 4 cut(s) 972, 1258, 1565, 2246
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 4 cut(s) 191, 1173, 1929, 2323
HphI GGTGA 3 cut(s) 827, 1273, 1691
Hpy166II GTNNAC 7 cut(s) 496, 586, 771, 1026, 1527, 2622, 2719
Hpy8I GTNNAC 7 cut(s) 496, 586, 771, 1026, 1527, 2622, 2719
Hpy99I CGWCG 1 cut(s) 1621
HpyCH4III ACNGT 6 cut(s) 90, 423, 1271, 2200, 2702, 2723
HpyCH4IV ACGT 1 cut(s) 1437
HpyF10VI GCNNNNNNNGC 3 cut(s) 1573, 1711, 2006
HpySE526I ACGT 1 cut(s) 1437
KpnI GGTACC 1 cut(s) 1107
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
LguI GCTCTTC 1 cut(s) 1323
LmnI GCTCC 7 cut(s) 910, 1625, 2089, 2186, 2400, 2785, 3012
Lsp1109I GCAGC 4 cut(s) 565, 1075, 1239, 1287
LweI GCATC 3 cut(s) 1044, 1701, 2539
MaeI CTAG 8 cut(s) 60, 222, 626, 737, 1401, 2754, 2879, 3025
MaeII ACGT 1 cut(s) 1437
MaeIII GTNAC 2 cut(s) 269, 1337
MalI GATC 9 cut(s) 189, 1350, 2017, 2106, 2315, 2616, 2772, 2815, 2946
MboI GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
MfeI CAATTG 4 cut(s) 166, 550, 719, 1411
MflI RGATCY 2 cut(s) 1348, 2770
MhlI GDGCHC 6 cut(s) 71, 915, 1630, 2191, 2520, 3017
MlsI TGGCCA 1 cut(s) 312
MluI ACGCGT 1 cut(s) 2273
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 5 cut(s) 274, 277, 2558, 2717, 2876
MmeI TCCRAC 6 cut(s) 119, 721, 1102, 1470, 1603, 1993
Mox20I TGGCCA 1 cut(s) 312
Mph1103I ATGCAT 2 cut(s) 1672, 2686
MroXI GAANNNNTTC 1 cut(s) 1129
MscI TGGCCA 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 4 cut(s) 578, 664, 1720, 2931
MspI CCGG 4 cut(s) 191, 1173, 1929, 2323
MunI CAATTG 4 cut(s) 166, 550, 719, 1411
Mva1269I GAATGC 2 cut(s) 1536, 2684
MvnI CGCG 1 cut(s) 2275
MwoI GCNNNNNNNGC 3 cut(s) 1573, 1711, 2006
NcoI CCATGG 2 cut(s) 321, 2191
NdeI CATATG 1 cut(s) 833
NdeII GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
NlaIV GGNNCC 8 cut(s) 1105, 1444, 1686, 2115, 2188, 2402, 2772, 2897
NmuCI GTSAC 1 cut(s) 269
NsiI ATGCAT 2 cut(s) 1672, 2686
NspI RCATGY 1 cut(s) 2392
NspV TTCGAA 1 cut(s) 728
PagI TCATGA 1 cut(s) 2407
PciSI GCTCTTC 1 cut(s) 1323
PctI GAATGC 2 cut(s) 1536, 2684
PdmI GAANNNNTTC 1 cut(s) 1129
PflMI CCANNNNNTGG 2 cut(s) 689, 1913
PkrI GCNGC 5 cut(s) 580, 1065, 1229, 1277, 1535
PleI GAGTC 5 cut(s) 274, 276, 2558, 2716, 2876
PpsI GAGTC 5 cut(s) 274, 276, 2558, 2716, 2876
PpuMI RGGWCCY 2 cut(s) 784, 1684
PshAI GACNNNNGTC 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 1784
Psp124BI GAGCTC 3 cut(s) 71, 915, 1630
Psp5II RGGWCCY 2 cut(s) 784, 1684
PspEI GGTNACC 1 cut(s) 1337
PspN4I GGNNCC 8 cut(s) 1105, 1444, 1686, 2115, 2188, 2402, 2772, 2897
PspPI GGNCC 7 cut(s) 193, 599, 784, 1443, 1684, 2114, 2896
PspPPI RGGWCCY 2 cut(s) 784, 1684
PsrI GAACNNNNNNTAC 4 cut(s) 479, 511, 1007, 1039
PstI CTGCAG 3 cut(s) 583, 783, 1635
PsuI RGATCY 2 cut(s) 1348, 2770
PvuII CAGCTG 3 cut(s) 578, 664, 2931
SacI GAGCTC 3 cut(s) 71, 915, 1630
SapI GCTCTTC 1 cut(s) 1323
SatI GCNGC 5 cut(s) 579, 1064, 1228, 1276, 1534
Sau3AI GATC 9 cut(s) 187, 1348, 2015, 2104, 2313, 2614, 2770, 2813, 2944
Sau96I GGNCC 7 cut(s) 193, 599, 784, 1443, 1684, 2114, 2896
SbfI CCTGCAGG 1 cut(s) 783
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 5 cut(s) 274, 277, 2558, 2717, 2876
SdaI CCTGCAGG 1 cut(s) 783
SduI GDGCHC 6 cut(s) 71, 915, 1630, 2191, 2520, 3017
SfaNI GCATC 3 cut(s) 1044, 1701, 2539
SfcI CTRYAG 6 cut(s) 579, 779, 1270, 1631, 1715, 2148
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 6 cut(s) 599, 784, 1443, 1684, 2114, 2896
SmlI CTYRAG 9 cut(s) 133, 265, 347, 427, 914, 969, 2068, 2741, 2884
SmoI CTYRAG 9 cut(s) 133, 265, 347, 427, 914, 969, 2068, 2741, 2884
SpeI ACTAGT 1 cut(s) 59
Sse8387I CCTGCAGG 1 cut(s) 783
SsiI CCGC 5 cut(s) 1533, 1720, 2009, 2432, 2458
SspMI CTAG 8 cut(s) 60, 222, 626, 737, 1401, 2754, 2879, 3025
SstI GAGCTC 3 cut(s) 71, 915, 1630
StyI CCWWGG 4 cut(s) 321, 354, 2191, 2305
TaaI ACNGT 6 cut(s) 90, 423, 1271, 2200, 2702, 2723
TaiI ACGT 1 cut(s) 1440
TaqI TCGA 3 cut(s) 728, 2021, 2107
TaqII GACCGA 1 cut(s) 725
TatI WGTACW 4 cut(s) 423, 554, 939, 2379
TauI GCSGC 1 cut(s) 1536
TscAI CASTG 9 cut(s) 110, 889, 1033, 1534, 1822, 1842, 2478, 2707, 2823
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 4 cut(s) 578, 1063, 1227, 1275
Tsp45I GTSAC 1 cut(s) 269
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 9 cut(s) 110, 889, 1033, 1534, 1822, 1842, 2478, 2707, 2823
Van91I CCANNNNNTGG 2 cut(s) 689, 1913
VpaK11BI GGWCC 6 cut(s) 599, 784, 1443, 1684, 2114, 2896
VspI ATTAAT 1 cut(s) 1784
XbaI TCTAGA 1 cut(s) 2878
XceI RCATGY 1 cut(s) 2392
XcmI CCANNNNNNNNNTGG 1 cut(s) 831
XmnI GAANNNNTTC 1 cut(s) 1129
XspI CTAG 8 cut(s) 60, 222, 626, 737, 1401, 2754, 2879, 3025
ZrmI AGTACT 1 cut(s) 425
Zsp2I ATGCAT 2 cut(s) 1672, 2686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.