MD17G1144300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011195
Physical Location & Seq
Forward (+)
13011794 .. 13015538
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1144300.v1.1.491

Sequence Viewer

Length: 3192 bp
ATGAAGCTTTACCCCTTCAATTTCTTCTTCTTCTTCTTCTTCTTCTTGTTATTCTGCTTCTCCGTGCCCATACCTATTGTATCTAGTTTGAGCTCTGATGGGGTGGCGCTGTTGTCCCTCCTCAAGCACTGGACTGTAATCCCATCCCCCATATCCTCCACCTGGAATGCCTTTGATTCCAATCCATGCCAATGGGTTGGAATCCAATGTGATAAATCCCACAATGTGGTTGCCTTAAACCTCACTGGGTTTGGAATTTCTGGCCAATTGGGGCCTCAAGTTGGCAGCTTTAGGTATCTGCAGACTCTTGCTTTGGGTTCCAATAGTATTTCTGGCAAAATCCCAGTTGGGTTGGCCAACTGTAGTTTACTTGAGTACTTGGACTTGTCAGAAAATGGATTTTCTGGTGAAATCCCTGAACCCTTGTTTTCGATTTCCAGCTTGGTTTATATATATCTGTATAAGAATAGTTTGAATGGTTCCATCCCTGCAAATGTTGGGAATTTGAGTAAATTGGAGGAGTTGTATTTGGATGACAACCATTTGAGTGGAGTCCTGCCTAAGAGTCTGAACAATCTTAGGAACCTAGTGTATCTTCAAGTTAGTGGTAATCGTCTCCACGGTAGGATTGATTTGGGTTCTGGGGATTGCAATAATTTGATTTTCTTGGATTTGTCACGCAATCAGTTTAGTGGAGGTCTTCCATCAAGTCTGGGAAATTGTAGTAATTTAACAATGTTTGGTGCTGTGAATAGCAACTTAACGGGGACTATCCCGTCTTCCCTTGGCCAACTAGAGAAGCTTGAACTTCTGCTCCTTCCTGAGAACCGTTTGTCTGGGAAAATACCTCCCGAACTTGGTAAATGCAAGTCCTTAACAGGACTACAGTTGTATTCAAACCAACTAGAGGGAGAAATTCCTAGTGAATTGGGGACGCTGAAATTGCAGGATCTTGAATTGTTTGAGAACAGGCTAACTGGTGAAATCCCGGTTAGCATTTGGAAGATTCAGAGTCTTCAGCAAATCCTTGTGTACAATAATAACCTCACCGGGGAGCTGCCTGTAGAGATGACTGAGCTGAAGCAACTGCAGAATATTTCGTTGTTTAACAACCTGTTTTCTGGAGTTATACCTCAAGGTCTGGGGATTAACAGCAGTTTAGTGCAGTTAGATTTTCTGAATAATAACTTCACTGGTACAATCCCTCCAAATCTTTGCCATGGAAAGAGGTTAAGGGTGTTGACTTTGGCTTCCAATCGACTTCAAGGTTCCATACCTTCTGGCGTTGGAAACTGCTCTACACTTTGGAGGTTGAAGCTTGAACAGAATAACCTGACTGGAGCTCTCCCGGAATTTGCAAAAAATCCAAACTTCGTTTATATGGACATCAGCAGCAATGAGATAAGTGGAGCCATTCCATCAAGCTTGCAAAACTGTGGCAACCTCACAACCATCAATTTGTCCACGAACAAGTTAACTGGACCTATACCGCAGGAGCTGGGGAAGCTTGCAGAGCTTCGTACTTTGGTTCTTTTCCAGAACAGCTTGGTTGGTTCTCTGGCACCTCAACTATCGAATTGTACCAAAATGGATAAGTTCGATGTGCGGTCCAATTTGTTGAATGGCTCCATTCCGTCAAGTCTGAGAAGCTGGACAGGTTTATCAACACTGATTTTAAGCGACAACAACTTTACTGGCGGCATCCCAACTTTCTTGTCGGAGTTCGAAAAGCTTTTAGAGCTACAACTTGGTGGAAATCTGTTCGGAGGTGTGATTCCATCATCAATTGGAACATTGCAGAGTATGTTTTATGCATTAAATCTTAGCAACAATGCATTGACAGGTCTGATTCCTCCAGAGCTAGGGAAGCTGATAAGGCTCCAACGATTAGATCTTTCTCATAACAATTTGACAGGGACTTTAAAAGTTCTCGGCGATATGAGTTCACTAACTGAGATTAATGTTTCAGACAACAACTTCACAGGTCCAGTACCAGAGACGTTGATGAACCTGTTGAGCTCATCCCCAGTGTCATTTTTGGGCAATCCCTACATATGTGTCAATTACCTTCAATCGTGTGGCTCAGTGTGCGCAGGAAACAACAGTTTTAAATCTTGCAACAGTCCACCAAGCAACCAGAAAGGCCTTAGTAAAGTGCAAATTGCATTTATAGCTCTAGGATCTTCGATGTTTGTTGTTTTTGTGCTTTATGGGCTGATTTATCTGTTCCTCTTCCACAAAAAGACCAAGCACGACTTTGAGATCTCTGCTCTGGAGGGTCCGTCTACCTTGCTCAACATGGTACTGGAGGCTACAGAAAACCTAAATGATCACTATATCATTGGGAGAGGAGCCCATGGAACAGTCTATAAGGCCTCTTTGGCTCCAGACAAAGATTATGCAATCAAGAAGCTTCAATTTGCAGGGCACAAAGGAAGGCGTTTGAGCATGATTAGAGAAATTCAAACACTTGGGTTGATTAGGCACCGGAATCTGGTTAGATTGGAAGACTTCTGGTTGGGAAAAGACCACGGTTTGATATTGTATCGGTACATGCAAAATGGGAGCCTTCATGATGTTCTACATGAAATCAAACCCCCGCCAACTCTCAAGTGGAATGTCTGCTATAGGATAGCGCTTGGAACTGCATACGGGTTGGAATATCTCCATTATGATTGTGATCCCCCTATAGTGCATCGAGACATCAAACCAATGAACATCCTCTTGGACTCTGACATGGAGCCCCATATCGGTGATTTTGGTATTGCGAAACTTTTGGATCATCAGTCTTCTGCAGCAACATCCATCGCAGTTGTGGGTACAACTGGATATATTGCACCAGAAAATGCATTTCGAACAGCAAAGAGCGTGGAATCTGACGTGTACAGTTATGGGGTTGTTTTACTTGAGCTGATAACAAGAAAGAAGGCATTGGATCCATCATTTATGGAGCAAACTGACATTGTAGAATGGGCTAGGTCAGTGTGGAGCAGCACGGAACAAATAGATCAGATGGTCGATTCAAGCCTTAAGGAGGAACTTCTGGATTCAAACATCATGGATCAAGTTATTGACGTGCTTATGGTGGCCTTCAGATGTACTGAGATAGATCCGAAAAGTAGACCCACAATGAGAGATGTGATCAAGCAATTGTTAGATGCAGATTCCAAAGTGAGAAGCGTAAAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1064

Amino Acids

116.85

Weight (kDa)

6.0

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 71 2.9e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 93 - 201 2.8e-07 Leucine-rich repeat region
LRR_8 PF13855 147 - 206 4.1e-06 Leucine rich repeat
LRR_8 PF13855 460 - 516 3.2e-07 Leucine rich repeat
Pkinase PF00069 778 - 1050 3.3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 780 - 1051 1.8e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 775
Acc16I TGCGCA 1 cut(s) 2092
AccB1I GGYRCC 2 cut(s) 1561, 2484
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 2 cut(s) 2285, 3121
AciI CCGC 4 cut(s) 1490, 1606, 1698, 2600
AclWI GGATC 8 cut(s) 957, 2188, 2675, 2787, 2930, 2943, 3069, 3104
AcoI YGGCCR 3 cut(s) 262, 354, 787
AcsI RAATTY 5 cut(s) 255, 502, 915, 1352, 2460
AcuI CTGAAG 3 cut(s) 1001, 1100, 3076
AdeI CACNNNGTG 1 cut(s) 226
AfeI AGCGCT 1 cut(s) 2637
AflII CTTAAG 1 cut(s) 3029
AflIII ACRYGT 1 cut(s) 2880
AjiI CACGTC 2 cut(s) 2881, 3076
AjnI CCWGG 1 cut(s) 161
AjuI GAANNNNNNNTTGG 2 cut(s) 2708, 2740
AloI GAACNNNNNNTCC 2 cut(s) 798, 830
Alw21I GWGCWC 3 cut(s) 95, 1345, 2021
Alw26I GTCTC 3 cut(s) 620, 1991, 2694
AlwI GGATC 8 cut(s) 957, 2188, 2675, 2787, 2930, 2943, 3069, 3104
AlwNI CAGNNNCTG 1 cut(s) 1498
Aor51HI AGCGCT 1 cut(s) 2637
AoxI GGCC 7 cut(s) 262, 272, 354, 787, 2143, 2373, 3087
ApeKI GCWGC 5 cut(s) 285, 1057, 1392, 2795, 2991
ApoI RAATTY 5 cut(s) 255, 502, 915, 1352, 2460
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AseI ATTAAT 1 cut(s) 1959
AspLEI GCGC 3 cut(s) 109, 2093, 2638
AspS9I GGNCC 5 cut(s) 272, 1481, 1608, 1985, 2279
AsuC2I CCSGG 3 cut(s) 989, 1051, 1349
AsuHPI GGTGA 4 cut(s) 419, 992, 1039, 2765
AsuII TTCGAA 2 cut(s) 1725, 2854
AvaII GGWCC 4 cut(s) 1481, 1608, 1985, 2279
BaeGI GKGCMC 2 cut(s) 69, 2430
BalI TGGCCA 3 cut(s) 264, 356, 789
BamHI GGATCC 1 cut(s) 2935
BanI GGYRCC 2 cut(s) 1561, 2484
BanII GRGCYC 5 cut(s) 95, 1345, 2021, 2356, 2745
BbsI GAAGAC 5 cut(s) 692, 771, 1007, 2514, 2781
Bbv12I GWGCWC 3 cut(s) 95, 1345, 2021
BbvI GCAGC 5 cut(s) 297, 1044, 1404, 2807, 3003
BciT130I CCWGG 1 cut(s) 163
BclI TGATCA 2 cut(s) 2329, 3141
BcnI CCSGG 3 cut(s) 989, 1051, 1349
BcoDI GTCTC 3 cut(s) 620, 1991, 2694
BfaI CTAG 9 cut(s) 84, 587, 794, 905, 921, 1862, 2177, 2976, 3190
BfmI CTRYAG 9 cut(s) 299, 361, 884, 1062, 1088, 2313, 2626, 2688, 2793
BfoI RGCGCY 2 cut(s) 110, 2639
BfrI CTTAAG 1 cut(s) 3029
BglI GCCNNNNNGGC 1 cut(s) 2381
BglII AGATCT 2 cut(s) 1891, 2262
BisI GCNGC 6 cut(s) 286, 1058, 1393, 1699, 2796, 2992
BlsI GCNGC 6 cut(s) 287, 1059, 1394, 1700, 2797, 2993
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 4 cut(s) 163, 989, 1051, 1349
Bme18I GGWCC 4 cut(s) 1481, 1608, 1985, 2279
BmgBI CACGTC 2 cut(s) 2881, 3076
BmgT120I GGNCC 5 cut(s) 272, 1481, 1608, 1985, 2279
BmrFI CCNGG 4 cut(s) 163, 989, 1051, 1349
BmrI ACTGGG 3 cut(s) 255, 338, 2021
BmsI GCATC 3 cut(s) 1710, 2704, 3148
BmuI ACTGGG 3 cut(s) 255, 338, 2021
BpiI GAAGAC 5 cut(s) 692, 771, 1007, 2514, 2781
BpmI CTGGAG 6 cut(s) 1143, 1359, 1839, 2294, 2327, 2370
Bpu14I TTCGAA 2 cut(s) 1725, 2854
BpuEI CTTGAG 6 cut(s) 107, 261, 392, 1119, 2594, 2927
BpuMI CCSGG 3 cut(s) 989, 1051, 1349
BsaBI GATNNNNATC 2 cut(s) 180, 2679
BsaJI CCNNGG 6 cut(s) 619, 784, 1050, 1219, 2356, 2530
BsaWI WCCGGW 1 cut(s) 2487
BsaXI ACNNNNNCTCC 8 cut(s) 509, 539, 798, 828, 1189, 1219, 2267, 2297
Bse3DI GCAATG 2 cut(s) 1402, 1793
Bse8I GATNNNNATC 2 cut(s) 180, 2679
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 6 cut(s) 619, 784, 1050, 1219, 2356, 2530
BseGI GGATG 7 cut(s) 143, 483, 538, 1701, 2021, 2718, 2801
BseJI GATNNNNATC 2 cut(s) 180, 2679
BseMI GCAATG 2 cut(s) 1402, 1793
BseMII CTCAG 6 cut(s) 813, 1065, 1634, 1944, 2097, 3093
BseRI GAGGAG 3 cut(s) 110, 533, 2364
BseSI GKGCMC 2 cut(s) 69, 2430
BseXI GCAGC 5 cut(s) 297, 1044, 1404, 2807, 3003
BseYI CCCAGC 1 cut(s) 1498
BsgI GTGCAG 1 cut(s) 1184
BshFI GGCC 7 cut(s) 264, 274, 356, 789, 2145, 2375, 3089
BshNI GGYRCC 2 cut(s) 1561, 2484
BsiHKAI GWGCWC 3 cut(s) 95, 1345, 2021
BsiSI CCGG 4 cut(s) 989, 1050, 1349, 2488
BslFI GGGAC 4 cut(s) 100, 781, 946, 1930
BsmAI GTCTC 3 cut(s) 620, 1991, 2694
BsmBI CGTCTC 2 cut(s) 620, 1991
BsmFI GGGAC 4 cut(s) 100, 781, 946, 1930
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 7 cut(s) 264, 274, 356, 789, 2145, 2375, 3089
Bsp119I TTCGAA 2 cut(s) 1725, 2854
Bsp1286I GDGCHC 7 cut(s) 69, 95, 1345, 2021, 2356, 2430, 2745
Bsp1407I TGTACA 2 cut(s) 1032, 2883
Bsp19I CCATGG 2 cut(s) 1219, 2356
BspACI CCGC 4 cut(s) 1490, 1606, 1698, 2600
BspANI GGCC 7 cut(s) 264, 274, 356, 789, 2145, 2375, 3089
BspCNI CTCAG 6 cut(s) 814, 1066, 1635, 1945, 2096, 3094
BspHI TCATGA 1 cut(s) 2572
BspMAI CTGCAG 3 cut(s) 303, 1092, 2797
BspPI GGATC 8 cut(s) 957, 2188, 2675, 2787, 2930, 2943, 3069, 3104
BspT104I TTCGAA 2 cut(s) 1725, 2854
BspT107I GGYRCC 2 cut(s) 1561, 2484
BspTI CTTAAG 1 cut(s) 3029
BsrDI GCAATG 2 cut(s) 1402, 1793
BsrGI TGTACA 2 cut(s) 1032, 2883
BssECI CCNNGG 6 cut(s) 619, 784, 1050, 1219, 2356, 2530
BssT1I CCWWGG 3 cut(s) 784, 1219, 2356
Bst2UI CCWGG 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 2237
BstAFI CTTAAG 1 cut(s) 3029
BstAUI TGTACA 2 cut(s) 1032, 2883
BstBI TTCGAA 2 cut(s) 1725, 2854
BstC8I GCNNGC 2 cut(s) 1427, 1509
BstDSI CCRYGG 4 cut(s) 619, 1219, 2356, 2530
BstENI CCTNNNNNAGG 1 cut(s) 3032
BstF5I GGATG 7 cut(s) 143, 483, 538, 1701, 2021, 2718, 2801
BstH2I RGCGCY 2 cut(s) 110, 2639
BstHHI GCGC 3 cut(s) 109, 2093, 2638
BstMAI GTCTC 3 cut(s) 620, 1991, 2694
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 2557
BstSCI CCNGG 4 cut(s) 161, 987, 1049, 1347
BstSFI CTRYAG 9 cut(s) 299, 361, 884, 1062, 1088, 2313, 2626, 2688, 2793
BstSLI GKGCMC 2 cut(s) 69, 2430
BstV1I GCAGC 5 cut(s) 297, 1044, 1404, 2807, 3003
BstV2I GAAGAC 5 cut(s) 692, 771, 1007, 2514, 2781
BstX2I RGATCY 6 cut(s) 949, 1891, 2180, 2262, 2935, 3109
BstXI CCANNNNNNTGG 3 cut(s) 192, 197, 548
BstYI RGATCY 6 cut(s) 949, 1891, 2180, 2262, 2935, 3109
BsuRI GGCC 7 cut(s) 264, 274, 356, 789, 2145, 2375, 3089
BtgI CCRYGG 4 cut(s) 619, 1219, 2356, 2530
BtgZI GCGATG 1 cut(s) 2791
BtrI CACGTC 2 cut(s) 2881, 3076
BtsCI GGATG 7 cut(s) 143, 483, 538, 1701, 2021, 2718, 2801
BtsIMutI CAGTG 7 cut(s) 127, 243, 1191, 1667, 2034, 2091, 2988
Cac8I GCNNGC 2 cut(s) 1427, 1509
CaiI CAGNNNCTG 1 cut(s) 1498
CciI TCATGA 1 cut(s) 2572
CfoI GCGC 3 cut(s) 109, 2093, 2638
Cfr13I GGNCC 5 cut(s) 272, 1481, 1608, 1985, 2279
CseI GACGC 1 cut(s) 943
CspCI CAANNNNNGTGG 2 cut(s) 2850, 2885
DraI TTTAAA 2 cut(s) 1923, 2110
DraIII CACNNNGTG 1 cut(s) 226
DrdI GACNNNNNNGTC 1 cut(s) 775
DseDI GACNNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 3 cut(s) 262, 354, 787
Eam1104I CTCTTC 1 cut(s) 2237
EarI CTCTTC 1 cut(s) 2237
Ecl136II GAGCTC 3 cut(s) 93, 1343, 2019
Eco130I CCWWGG 3 cut(s) 784, 1219, 2356
Eco147I AGGCCT 2 cut(s) 2145, 2375
Eco24I GRGCYC 5 cut(s) 95, 1345, 2021, 2356, 2745
Eco47I GGWCC 4 cut(s) 1481, 1608, 1985, 2279
Eco47III AGCGCT 1 cut(s) 2637
Eco53kI GAGCTC 3 cut(s) 93, 1343, 2019
Eco57I CTGAAG 3 cut(s) 1001, 1100, 3076
EcoICRI GAGCTC 3 cut(s) 93, 1343, 2019
EcoNI CCTNNNNNAGG 1 cut(s) 3032
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 3 cut(s) 784, 1219, 2356
EcoT22I ATGCAT 3 cut(s) 1816, 1837, 2851
EcoT38I GRGCYC 5 cut(s) 95, 1345, 2021, 2356, 2745
ErhI CCWWGG 3 cut(s) 784, 1219, 2356
Esp3I CGTCTC 2 cut(s) 620, 1991
FalI AAGNNNNNCTT 2 cut(s) 2240, 2272
FaqI GGGAC 4 cut(s) 100, 781, 946, 1930
FauI CCCGC 1 cut(s) 2607
FauNDI CATATG 1 cut(s) 2054
FbaI TGATCA 2 cut(s) 2329, 3141
FblI GTMKAC 2 cut(s) 2285, 3121
Fnu4HI GCNGC 6 cut(s) 286, 1058, 1393, 1699, 2796, 2992
FokI GGATG 7 cut(s) 130, 470, 545, 1688, 2008, 2705, 2788
FriOI GRGCYC 5 cut(s) 95, 1345, 2021, 2356, 2745
Fsp4HI GCNGC 6 cut(s) 286, 1058, 1393, 1699, 2796, 2992
FspBI CTAG 9 cut(s) 84, 587, 794, 905, 921, 1862, 2177, 2976, 3190
FspI TGCGCA 1 cut(s) 2092
GlaI GCGC 3 cut(s) 108, 2092, 2637
GluI GCNGC 6 cut(s) 286, 1058, 1393, 1699, 2796, 2992
GsaI CCCAGC 1 cut(s) 1502
GsuI CTGGAG 6 cut(s) 1143, 1359, 1839, 2294, 2327, 2370
HaeII RGCGCY 2 cut(s) 110, 2639
HaeIII GGCC 7 cut(s) 264, 274, 356, 789, 2145, 2375, 3089
HapII CCGG 4 cut(s) 989, 1050, 1349, 2488
HgaI GACGC 1 cut(s) 943
HhaI GCGC 3 cut(s) 109, 2093, 2638
Hin6I GCGC 3 cut(s) 107, 2091, 2636
HinP1I GCGC 3 cut(s) 107, 2091, 2636
HincII GTYRAC 2 cut(s) 1242, 1476
HindII GTYRAC 2 cut(s) 1242, 1476
HindIII AAGCTT 7 cut(s) 5, 800, 1316, 1423, 1505, 1730, 2411
HpaI GTTAAC 1 cut(s) 1476
HpaII CCGG 4 cut(s) 989, 1050, 1349, 2488
HphI GGTGA 4 cut(s) 419, 992, 1039, 2765
HpyAV CCTTC 8 cut(s) 25, 827, 1287, 2078, 2430, 2579, 2920, 3100
HpyCH4IV ACGT 3 cut(s) 2000, 2880, 3075
HpySE526I ACGT 3 cut(s) 2000, 2880, 3075
HspAI GCGC 3 cut(s) 107, 2091, 2636
Ksp22I TGATCA 2 cut(s) 2329, 3141
KspAI GTTAAC 1 cut(s) 1476
Lsp1109I GCAGC 5 cut(s) 297, 1044, 1404, 2807, 3003
LweI GCATC 3 cut(s) 1710, 2704, 3148
MaeI CTAG 9 cut(s) 84, 587, 794, 905, 921, 1862, 2177, 2976, 3190
MaeII ACGT 3 cut(s) 2000, 2880, 3075
MaeIII GTNAC 1 cut(s) 675
MfeI CAATTG 3 cut(s) 266, 1785, 3149
MflI RGATCY 6 cut(s) 949, 1891, 2180, 2262, 2935, 3109
MhlI GDGCHC 7 cut(s) 69, 95, 1345, 2021, 2356, 2430, 2745
MlsI TGGCCA 3 cut(s) 264, 356, 789
MluNI TGGCCA 3 cut(s) 264, 356, 789
MlyI GAGTC 5 cut(s) 298, 561, 574, 1021, 2723
MmeI TCCRAC 5 cut(s) 178, 1267, 1698, 1906, 2637
Mox20I TGGCCA 3 cut(s) 264, 356, 789
Mph1103I ATGCAT 3 cut(s) 1816, 1837, 2851
MscI TGGCCA 3 cut(s) 264, 356, 789
MslI CAYNNNNRTG 3 cut(s) 190, 546, 2751
Msp20I TGGCCA 3 cut(s) 264, 356, 789
MspCI CTTAAG 1 cut(s) 3029
MspI CCGG 4 cut(s) 989, 1050, 1349, 2488
MspR9I CCNGG 4 cut(s) 163, 989, 1051, 1349
MunI CAATTG 3 cut(s) 266, 1785, 3149
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 1 cut(s) 163
NciI CCSGG 3 cut(s) 989, 1051, 1349
NcoI CCATGG 2 cut(s) 1219, 2356
NdeI CATATG 1 cut(s) 2054
NmeAIII GCCGAG 1 cut(s) 1911
NmuCI GTSAC 1 cut(s) 675
NsbI TGCGCA 1 cut(s) 2092
NsiI ATGCAT 3 cut(s) 1816, 1837, 2851
NspI RCATGY 1 cut(s) 2557
NspV TTCGAA 2 cut(s) 1725, 2854
PagI TCATGA 1 cut(s) 2572
PceI AGGCCT 2 cut(s) 2145, 2375
PctI GAATGC 1 cut(s) 172
PflMI CCANNNNNTGG 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 1347
PkrI GCNGC 6 cut(s) 287, 1059, 1394, 1700, 2797, 2993
PleI GAGTC 5 cut(s) 298, 560, 573, 1020, 2723
PpsI GAGTC 5 cut(s) 298, 560, 573, 1020, 2723
PshBI ATTAAT 1 cut(s) 1959
Psp124BI GAGCTC 3 cut(s) 95, 1345, 2021
Psp6I CCWGG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 1498
PspGI CCWGG 1 cut(s) 161
PspPI GGNCC 5 cut(s) 272, 1481, 1608, 1985, 2279
PstI CTGCAG 3 cut(s) 303, 1092, 2797
PstNI CAGNNNCTG 1 cut(s) 1498
PsuI RGATCY 6 cut(s) 949, 1891, 2180, 2262, 2935, 3109
RseI CAYNNNNRTG 3 cut(s) 190, 546, 2751
SacI GAGCTC 3 cut(s) 95, 1345, 2021
SatI GCNGC 6 cut(s) 286, 1058, 1393, 1699, 2796, 2992
Sau96I GGNCC 5 cut(s) 272, 1481, 1608, 1985, 2279
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 5 cut(s) 298, 561, 574, 1021, 2723
ScrFI CCNGG 4 cut(s) 163, 989, 1051, 1349
SduI GDGCHC 7 cut(s) 69, 95, 1345, 2021, 2356, 2430, 2745
SfaNI GCATC 3 cut(s) 1710, 2704, 3148
SfcI CTRYAG 9 cut(s) 299, 361, 884, 1062, 1088, 2313, 2626, 2688, 2793
SfuI TTCGAA 2 cut(s) 1725, 2854
SinI GGWCC 4 cut(s) 1481, 1608, 1985, 2279
SmiMI CAYNNNNRTG 3 cut(s) 190, 546, 2751
SmlI CTYRAG 7 cut(s) 122, 276, 371, 1134, 2609, 2906, 3029
SmoI CTYRAG 7 cut(s) 122, 276, 371, 1134, 2609, 2906, 3029
SseBI AGGCCT 2 cut(s) 2145, 2375
SsiI CCGC 4 cut(s) 1490, 1606, 1698, 2600
SspI AATATT 1 cut(s) 1096
SspMI CTAG 9 cut(s) 84, 587, 794, 905, 921, 1862, 2177, 2976, 3190
SstI GAGCTC 3 cut(s) 95, 1345, 2021
StuI AGGCCT 2 cut(s) 2145, 2375
StyD4I CCNGG 4 cut(s) 161, 987, 1049, 1347
StyI CCWWGG 3 cut(s) 784, 1219, 2356
TaiI ACGT 3 cut(s) 2003, 2883, 3078
TaqI TCGA 9 cut(s) 431, 1258, 1574, 1599, 1725, 2186, 2698, 2854, 3018
TatI WGTACW 4 cut(s) 375, 1032, 2883, 3098
TauI GCSGC 1 cut(s) 1701
TscAI CASTG 7 cut(s) 134, 250, 1198, 1674, 2034, 2091, 2988
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 5 cut(s) 285, 1057, 1392, 2795, 2991
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 5 cut(s) 17, 2021, 2561, 2601, 2729
TspGWI ACGGA 4 cut(s) 52, 1623, 2271, 3011
TspRI CASTG 7 cut(s) 134, 250, 1198, 1674, 2034, 2091, 2988
Van91I CCANNNNNTGG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 3029
VpaK11BI GGWCC 4 cut(s) 1481, 1608, 1985, 2279
VspI ATTAAT 1 cut(s) 1959
XagI CCTNNNNNAGG 1 cut(s) 3032
XapI RAATTY 5 cut(s) 255, 502, 915, 1352, 2460
XceI RCATGY 1 cut(s) 2557
XcmI CCANNNNNNNNNTGG 2 cut(s) 2610, 2812
XmiI GTMKAC 2 cut(s) 2285, 3121
XspI CTAG 9 cut(s) 84, 587, 794, 905, 921, 1862, 2177, 2976, 3190
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 3 cut(s) 1816, 1837, 2851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.