pycom09g07690
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011195
Physical Location & Seq
Forward (+)
5837865 .. 5841142
3278 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07690.1

Sequence Viewer

Length: 3186 bp
ATGCAGCTTTCTCTCTCAAATTTCTTCTTCTTATTGTTATTCTTCTTTTCTGTCCCCATACCTCTTGTATCTAGCTTGAGCTCTGATGGGGTGGCAATGTTGTCCCTCCTCAAGCACTGGACTATAGTCCCAACCCCAATATCCTCCACCTGGAATGCCTCTGATTCCAATCCTTGCCAATGGGTTGGAATCCAATGTGATAAATCCCACAATGTGGTTGCCTTAAACCTCACCGGGTTTGGAATTTCTGGCCAATTGGGTCCTGAAGTTGGCAGCTTTAGATTTTTGCAGACTCTTTCTTTGGGTTCCAACAACTTTAGTGGCAAAATCCCAACCGGGTTGGCCAATTGTACTTTCCTTGAGTACTTGGATTTGTCAGTAAATGGTTTTTCTGGTGAAATCCCTGAACCCTTGTTTTCGATTTCCAGCTTGGTCCATATTAATCTGTATGAGAATAGTTTGAATGGTTCCATCCCTGCAAATGTTGGGAATTTAAGTAAATTGGAGAAGTTGTATTTGGGTTATAACCAGTTGAGTGGAGTCCTGCCTAAGAGTCTGAACAATCTTGGGAACCTAGTGTATCTTGACGTTAGGGGGAATCATCTCGAGGGTAGGATTGATTTGGGTTCTGGGGATTGCAAGAATTTGTTTTTCTTGGATTTGTCACGCAATCAGTTTAGTGGAGGTCTTCCATCAACCCTGGGAAATTGTAGTAATTTAACACAGTTTGCTGCTGTGAGTAGCAACTTAATGGGGACTATCCCGTCGTCCTTTGGCCAACTAGGGAAGCTTGAACTTCTGTACCTTCCTGAGAACCGTTTGTCTGGGAAAATACCTCCTGAACTTAGTAATTGTACGTCCTTGACCGGACTACAGTTGTATACGAACCAACTAGAGGGAGAAATTCCTAGTGAATTGGGGCTGCTGACCAAATTGCAGGATCTTGAATTGTTTGAGAACAGGTTAACCGGTGAAATCCCAATTAGCATTTGGAAGATTCAGAGTCTTCAGCACATCCTTGTGTACAATAATAGCCTCACGGGGGAGCTGCCTGTGGAGATGACTGAGCTGAAGCAACTGCAGAATATTTCGTTGTTTAACAACCTGTTTTCTGGAGTTATACCTCAAGGTCTGGGGATTAACAGCAGTCTAGTGCAGTTAGATTTTATGTATAATAACTTCACTGGTACAATCCCTCCAAATCTTTGCCATGGAAAGCGCTTAAGGGTGTTGACTATGGCTTACAATCGACTTCAAGGTTCCATACCTTCTGATGTTGGAAATTGCCCCACTCTCCGGAGGTTGAAGCTTGAACGGAATAACCTCAGTGGAGCTCTCCCGGAATTTGCAGAAAATCCAAACTTCGATCATATGGACATCAGCAGCAATGAGATTAGTGGAGCAATTCCATCAAGCTTGCGAAACTGTGGCAACCTCACAACTATCAATTTGTCCACAAACAAGTTAACCGGAGCTATACCGCCGGAGCTGGGGAATCTTGCAAAGCTCCGCACTTTGGTTCTTTTCCACAACAATTTGGTTGGTTCTCTGCCTCCTCAACTATCCAAGTGTACCAAAATGGATAAGTTTGATGTGCGGTCCAACTTGTTGAACGGCTCCATTCCGTCAATTCTGACAAGTTGGACAGGTTTATCAACGCTGATTTTAAGCGACAACAGCTTCACTGGTGGCATCCCAACTTTCTTGTCGGAGTTCGAAAAGCTTTCAGAGCTACAGCTTGGTGGAAATCTGTTCGGAGGTGTGATTCCACCATCAATTGGAGCATTGAAGAGTATCTTTTATGCTTTAAATCTTAGCAACAATGGATTGACAGGTCCCCTTCCTGCAGAGCTAGGGCAGCTGATAAGGCTACAACAATTAGATCTTTCTCGTAACAATTTGACAGGGACTTTAAAAGCGCTCGACGGTATGAGTTCACTAACTGAGGTTCATGTTTCAGACAACAACTTCACAGGTCCGGTACCGGAGACGTTGATGAAGCTGTTGAACTCATCCCAATCGTCATTTTTGGGCAATCCCTACATATGTGTCAATTACCTTCCTTCATGTGGCTCATCGTGCGCGGGAAACAACAGTTTTAAGCCTTGCAACAGCCCACCAAGCAACCGGAAAGGCCTTGGTAAAGTGCAAATTGCATTGACAGCTCTAGGATCCTCGATGTTTGTTGTTTTTGTGCTTTATGGGCTGATTTATCTGTTCCTCTGGCGCAAAGGGGCCAAGCACGATCTTGAGATCTCTGCTCAAGAGGGGCCATCTGCCTTGCTCGACAAGGTGCTGGAGGCTACAGAAAACCTGAATGGTCACTATATCATCGGGAGAGGAGCCCATGGAACAGTCTATAAGGCCTCCTTGGCGACAGACCAAGATTATGCAGTCAAGAAGCTTCAATTTGCGGGGCACGAAGGAAGGCGTTTGAGCATGGTTCGAGAAATTCAAACCCTTGGGTCAATTAGGCACCGGAATCTGGTTAGATTGGAAGACTTCTGGTTAAGAAACGACCACGGTTTAATCTTGTATAGGTACATGGAAAATGGGAGCCTTCATGATGTTTTACATGAAATTGAACCCCAACCAACTCTTGAGTGGAGTGTTCGCTGCCGGATAGCGCTTGGAACTGCATACGGGTTGGAATATCTCCATTACGATTGTGATCCCCCGATAGTGCATCGAGACATCAAACCTATGAACATCCTCTTGGACTCTGATATGGAGCCCCATATTGCTGACTTTGGTATTGCTAAACTTCTGGATCACCGGTCTTCTTCACCAACGACATCCATTGCAGTTGTGGGTACAACCGGATATATTGCACCAGAAAATGCAGTTACAACAGCAAGGGGCGTGGAATCTGACGTTTACAGTTACGGGGTCGTTTTACTTGAGCTGCTAACTAGAAAGAAGGCATTGGATCCATCATTTATGGAGCAAACTGACATTGCAGGATGGGCTAAGTCAGTGTGGAGCAGCACGGAACAAATTGATCAGATCGTCGATTCAAGCCTTAAGGAGGAACTTCTGGATTCAACCACCATGGATCAAGTTATCGACGTGCTTATGGTGGCTTTCAGATGTACTGAGAATGATCCGAAGAAGAGACCCACGATGAGAGCTGTTATCAAGCAATTGTTAGATGCGGATTCCCAAGTGAGAAGCACAAAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1062

Amino Acids

116.04

Weight (kDa)

5.83

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 5.1e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 196 5.5e-06 Leucine-rich repeat region
LRR_8 PF13855 143 - 202 4.5e-06 Leucine rich repeat
LRR_14 PF23598 216 - 317 2.5e-06 Leucine-rich repeat region
LRR_8 PF13855 456 - 513 1.1e-07 Leucine rich repeat
Pkinase PF00069 777 - 1047 1.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 777 - 1049 3.9e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AasI GACNNNNNNGTC 1 cut(s) 763
Acc65I GGTACC 1 cut(s) 1981
AccB1I GGYRCC 2 cut(s) 1981, 2475
AccB7I CCANNNNNTGG 2 cut(s) 214, 1778
AccI GTMKAC 1 cut(s) 881
AccII CGCG 1 cut(s) 2084
AccIII TCCGGA 1 cut(s) 1296
AciI CCGC 6 cut(s) 1481, 1510, 1597, 2084, 2414, 3155
AclWI GGATC 9 cut(s) 948, 2166, 2179, 2666, 2778, 2924, 2937, 3063, 3098
AcoI YGGCCR 3 cut(s) 250, 342, 775
AcsI RAATTY 7 cut(s) 19, 243, 490, 643, 903, 1343, 2451
AcuI CTGAAG 3 cut(s) 285, 992, 1091
AdeI CACNNNGTG 1 cut(s) 214
AfeI AGCGCT 3 cut(s) 1220, 1920, 2628
AflII CTTAAG 2 cut(s) 1222, 3023
AgeI ACCGGT 2 cut(s) 968, 2775
AjiI CACGTC 1 cut(s) 3070
AjnI CCWGG 2 cut(s) 149, 699
AjuI GAANNNNNNNTTGG 6 cut(s) 338, 370, 500, 532, 2699, 2731
Alw21I GWGCWC 2 cut(s) 83, 1336
Alw26I GTCTC 3 cut(s) 1982, 2685, 3109
AlwI GGATC 9 cut(s) 948, 2166, 2179, 2666, 2778, 2924, 2937, 3063, 3098
Ama87I CYCGRG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 1296
Aor51HI AGCGCT 3 cut(s) 1220, 1920, 2628
AoxI GGCC 7 cut(s) 250, 342, 775, 2134, 2235, 2270, 2364
ApoI RAATTY 7 cut(s) 19, 243, 490, 643, 903, 1343, 2451
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AseI ATTAAT 1 cut(s) 441
AsiGI ACCGGT 2 cut(s) 968, 2775
Asp718I GGTACC 1 cut(s) 1981
AspLEI GCGC 5 cut(s) 1221, 1921, 2084, 2229, 2629
AspS9I GGNCC 7 cut(s) 260, 433, 1599, 1835, 1976, 2235, 2270
AsuC2I CCSGG 3 cut(s) 235, 337, 1340
AsuHPI GGTGA 5 cut(s) 223, 407, 983, 2765, 2778
AsuII TTCGAA 1 cut(s) 1716
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 5 cut(s) 260, 433, 1599, 1835, 1976
BaeGI GKGCMC 1 cut(s) 2421
BaeI ACNNNNGTAYC 2 cut(s) 785, 818
BalI TGGCCA 3 cut(s) 252, 344, 777
BamHI GGATCC 2 cut(s) 2171, 2929
BanI GGYRCC 2 cut(s) 1981, 2475
BanII GRGCYC 4 cut(s) 83, 1336, 2347, 2736
BarI GAAGNNNNNNTAC 2 cut(s) 1163, 1195
BbsI GAAGAC 4 cut(s) 680, 998, 2505, 2772
Bbv12I GWGCWC 2 cut(s) 83, 1336
BccI CCATC 8 cut(s) 80, 479, 700, 1417, 1780, 2281, 2941, 2958
BceAI ACGGC 1 cut(s) 1630
BciT130I CCWGG 2 cut(s) 151, 701
BclI TGATCA 1 cut(s) 3001
BcnI CCSGG 3 cut(s) 235, 337, 1340
BcoDI GTCTC 3 cut(s) 1982, 2685, 3109
BfmI CTRYAG 6 cut(s) 123, 872, 1079, 1733, 1845, 2304
BfoI RGCGCY 3 cut(s) 1222, 1922, 2630
BfrI CTTAAG 2 cut(s) 1222, 3023
BglI GCCNNNNNGGC 1 cut(s) 2372
BglII AGATCT 2 cut(s) 1882, 2253
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 5 cut(s) 151, 235, 337, 701, 1340
Bme18I GGWCC 5 cut(s) 260, 433, 1599, 1835, 1976
BmeT110I CYCGRG 1 cut(s) 605
BmgBI CACGTC 1 cut(s) 3070
BmgT120I GGNCC 7 cut(s) 260, 433, 1599, 1835, 1976, 2235, 2270
BmrFI CCNGG 5 cut(s) 151, 235, 337, 701, 1340
BmsI GCATC 3 cut(s) 1701, 2695, 3142
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 4 cut(s) 680, 998, 2505, 2772
BpmI CTGGAG 2 cut(s) 1134, 2318
Bpu14I TTCGAA 1 cut(s) 1716
BpuEI CTTGAG 8 cut(s) 95, 97, 380, 1110, 2247, 2270, 2621, 2921
BpuMI CCSGG 3 cut(s) 235, 337, 1340
BsaBI GATNNNNATC 2 cut(s) 168, 2670
BsaI GGTCTC 1 cut(s) 3109
BsaJI CCNNGG 9 cut(s) 699, 700, 1210, 2137, 2347, 2370, 2461, 2521, 3051
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 1180, 1210
Bse118I RCCGGY 2 cut(s) 968, 2775
Bse1I ACTGG 4 cut(s) 122, 529, 1189, 1690
Bse3DI GCAATG 4 cut(s) 102, 1393, 2799, 2955
Bse8I GATNNNNATC 2 cut(s) 168, 2670
BseAI TCCGGA 1 cut(s) 1296
BseBI CCWGG 2 cut(s) 151, 701
BseDI CCNNGG 9 cut(s) 699, 700, 1210, 2137, 2347, 2370, 2461, 2521, 3051
BseGI GGATG 7 cut(s) 471, 1014, 1692, 2012, 2709, 2795, 2969
BseJI GATNNNNATC 2 cut(s) 168, 2670
BseMI GCAATG 4 cut(s) 102, 1393, 2799, 2955
BseMII CTCAG 5 cut(s) 801, 1056, 1339, 1935, 3087
BseNI ACTGG 4 cut(s) 122, 529, 1189, 1690
BseRI GAGGAG 3 cut(s) 98, 1545, 2355
BseSI GKGCMC 1 cut(s) 2421
BseYI CCCAGC 1 cut(s) 1489
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 1 cut(s) 2084
BshFI GGCC 7 cut(s) 252, 344, 777, 2136, 2237, 2272, 2366
BshNI GGYRCC 2 cut(s) 1981, 2475
BshTI ACCGGT 2 cut(s) 968, 2775
BsiHKAI GWGCWC 2 cut(s) 83, 1336
BsiHKCI CYCGRG 1 cut(s) 605
BslFI GGGAC 6 cut(s) 38, 88, 113, 769, 1821, 1921
BsmAI GTCTC 3 cut(s) 1982, 2685, 3109
BsmBI CGTCTC 1 cut(s) 1982
BsmFI GGGAC 6 cut(s) 38, 88, 113, 769, 1821, 1921
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 7 cut(s) 252, 344, 777, 2136, 2237, 2272, 2366
Bso31I GGTCTC 1 cut(s) 3109
BsoBI CYCGRG 1 cut(s) 605
Bsp119I TTCGAA 1 cut(s) 1716
Bsp1286I GDGCHC 5 cut(s) 83, 1336, 2347, 2421, 2736
Bsp13I TCCGGA 1 cut(s) 1296
Bsp1407I TGTACA 1 cut(s) 1023
Bsp19I CCATGG 3 cut(s) 1210, 2347, 3051
BspACI CCGC 6 cut(s) 1481, 1510, 1597, 2084, 2414, 3155
BspANI GGCC 7 cut(s) 252, 344, 777, 2136, 2237, 2272, 2366
BspCNI CTCAG 5 cut(s) 802, 1057, 1338, 1936, 3088
BspEI TCCGGA 1 cut(s) 1296
BspFNI CGCG 1 cut(s) 2084
BspHI TCATGA 1 cut(s) 2563
BspMAI CTGCAG 2 cut(s) 1083, 1849
BspPI GGATC 9 cut(s) 948, 2166, 2179, 2666, 2778, 2924, 2937, 3063, 3098
BspT104I TTCGAA 1 cut(s) 1716
BspT107I GGYRCC 2 cut(s) 1981, 2475
BspTI CTTAAG 2 cut(s) 1222, 3023
BspTNI GGTCTC 1 cut(s) 3109
BsrDI GCAATG 4 cut(s) 102, 1393, 2799, 2955
BsrFI RCCGGY 2 cut(s) 968, 2775
BsrGI TGTACA 1 cut(s) 1023
BsrI ACTGG 4 cut(s) 122, 529, 1189, 1690
BssAI RCCGGY 2 cut(s) 968, 2775
BssECI CCNNGG 9 cut(s) 699, 700, 1210, 2137, 2347, 2370, 2461, 2521, 3051
BssNAI GTATAC 1 cut(s) 882
BssT1I CCWWGG 6 cut(s) 1210, 2137, 2347, 2370, 2461, 3051
Bst1107I GTATAC 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 151, 701
Bst4CI ACNGT 9 cut(s) 726, 818, 876, 1427, 1928, 2096, 2356, 2525, 2882
Bst6I CTCTTC 2 cut(s) 1784, 3107
BstAFI CTTAAG 2 cut(s) 1222, 3023
BstAUI TGTACA 1 cut(s) 1023
BstBI TTCGAA 1 cut(s) 1716
BstC8I GCNNGC 1 cut(s) 1418
BstDEI CTNAG 9 cut(s) 549, 810, 845, 1065, 1325, 1814, 1944, 2970, 3096
BstDSI CCRYGG 4 cut(s) 1210, 2347, 2521, 3051
BstENI CCTNNNNNAGG 1 cut(s) 3026
BstF5I GGATG 7 cut(s) 471, 1014, 1692, 2012, 2709, 2795, 2969
BstFNI CGCG 1 cut(s) 2084
BstH2I RGCGCY 3 cut(s) 1222, 1922, 2630
BstHHI GCGC 5 cut(s) 1221, 1921, 2084, 2229, 2629
BstMAI GTCTC 3 cut(s) 1982, 2685, 3109
BstNI CCWGG 2 cut(s) 151, 701
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 5 cut(s) 149, 233, 335, 699, 1338
BstSFI CTRYAG 6 cut(s) 123, 872, 1079, 1733, 1845, 2304
BstSLI GKGCMC 1 cut(s) 2421
BstUI CGCG 1 cut(s) 2084
BstV2I GAAGAC 4 cut(s) 680, 998, 2505, 2772
BstX2I RGATCY 5 cut(s) 940, 1882, 2171, 2253, 2929
BstXI CCANNNNNNTGG 2 cut(s) 185, 536
BstYI RGATCY 5 cut(s) 940, 1882, 2171, 2253, 2929
BstZ17I GTATAC 1 cut(s) 882
BsuRI GGCC 7 cut(s) 252, 344, 777, 2136, 2237, 2272, 2366
BtgI CCRYGG 4 cut(s) 1210, 2347, 2521, 3051
BtrI CACGTC 1 cut(s) 3070
BtsCI GGATG 7 cut(s) 471, 1014, 1692, 2012, 2709, 2795, 2969
BtsIMutI CAGTG 5 cut(s) 115, 1182, 1333, 1683, 2982
Cac8I GCNNGC 1 cut(s) 1418
CciI TCATGA 1 cut(s) 2563
CfoI GCGC 5 cut(s) 1221, 1921, 2084, 2229, 2629
Cfr10I RCCGGY 2 cut(s) 968, 2775
Cfr13I GGNCC 7 cut(s) 260, 433, 1599, 1835, 1976, 2235, 2270
CspAI ACCGGT 2 cut(s) 968, 2775
CspCI CAANNNNNGTGG 4 cut(s) 301, 336, 2844, 2879
CviAII CATG 9 cut(s) 1211, 1952, 2067, 2348, 2440, 2545, 2564, 2576, 3052
DdeI CTNAG 9 cut(s) 549, 810, 845, 1065, 1325, 1814, 1944, 2970, 3096
DraI TTTAAA 2 cut(s) 1809, 1914
DraIII CACNNNGTG 1 cut(s) 214
DrdI GACNNNNNNGTC 1 cut(s) 763
DseDI GACNNNNNNGTC 1 cut(s) 763
EaeI YGGCCR 3 cut(s) 250, 342, 775
Eam1104I CTCTTC 2 cut(s) 1784, 3107
EarI CTCTTC 2 cut(s) 1784, 3107
Ecl136II GAGCTC 2 cut(s) 81, 1334
Eco130I CCWWGG 6 cut(s) 1210, 2137, 2347, 2370, 2461, 3051
Eco147I AGGCCT 2 cut(s) 2136, 2366
Eco24I GRGCYC 4 cut(s) 83, 1336, 2347, 2736
Eco31I GGTCTC 1 cut(s) 3109
Eco47I GGWCC 5 cut(s) 260, 433, 1599, 1835, 1976
Eco47III AGCGCT 3 cut(s) 1220, 1920, 2628
Eco53kI GAGCTC 2 cut(s) 81, 1334
Eco57I CTGAAG 3 cut(s) 285, 992, 1091
Eco88I CYCGRG 1 cut(s) 605
EcoICRI GAGCTC 2 cut(s) 81, 1334
EcoNI CCTNNNNNAGG 1 cut(s) 3026
EcoO109I RGGNCCY 2 cut(s) 260, 1835
EcoRII CCWGG 2 cut(s) 149, 699
EcoT14I CCWWGG 6 cut(s) 1210, 2137, 2347, 2370, 2461, 3051
EcoT38I GRGCYC 4 cut(s) 83, 1336, 2347, 2736
ErhI CCWWGG 6 cut(s) 1210, 2137, 2347, 2370, 2461, 3051
Esp3I CGTCTC 1 cut(s) 1982
FaeI CATG 9 cut(s) 1214, 1955, 2070, 2351, 2443, 2548, 2567, 2579, 3055
FalI AAGNNNNNCTT 4 cut(s) 1781, 1813, 2354, 2386
FaqI GGGAC 6 cut(s) 38, 88, 113, 769, 1821, 1921
FatI CATG 9 cut(s) 1210, 1951, 2066, 2347, 2439, 2544, 2563, 2575, 3051
FauI CCCGC 2 cut(s) 2077, 2407
FauNDI CATATG 2 cut(s) 1371, 2045
FbaI TGATCA 1 cut(s) 3001
FblI GTMKAC 1 cut(s) 881
FokI GGATG 7 cut(s) 458, 1001, 1679, 1999, 2696, 2782, 2976
FriOI GRGCYC 4 cut(s) 83, 1336, 2347, 2736
GlaI GCGC 5 cut(s) 1220, 1920, 2083, 2228, 2628
GsaI CCCAGC 1 cut(s) 1493
GsuI CTGGAG 2 cut(s) 1134, 2318
HaeII RGCGCY 3 cut(s) 1222, 1922, 2630
HaeIII GGCC 7 cut(s) 252, 344, 777, 2136, 2237, 2272, 2366
HhaI GCGC 5 cut(s) 1221, 1921, 2084, 2229, 2629
Hin1II CATG 9 cut(s) 1214, 1955, 2070, 2351, 2443, 2548, 2567, 2579, 3055
Hin6I GCGC 5 cut(s) 1219, 1919, 2082, 2227, 2627
HinP1I GCGC 5 cut(s) 1219, 1919, 2082, 2227, 2627
HincII GTYRAC 3 cut(s) 966, 1233, 1467
HindII GTYRAC 3 cut(s) 966, 1233, 1467
HindIII AAGCTT 5 cut(s) 788, 1307, 1414, 1721, 2402
HpaI GTTAAC 2 cut(s) 966, 1467
HphI GGTGA 5 cut(s) 223, 407, 983, 2765, 2778
Hpy166II GTNNAC 9 cut(s) 882, 966, 1024, 1233, 1455, 1467, 1572, 1937, 2878
Hpy8I GTNNAC 9 cut(s) 882, 966, 1024, 1233, 1455, 1467, 1572, 1937, 2878
Hpy99I CGWCG 4 cut(s) 769, 1928, 3014, 3071
HpyAV CCTTC 9 cut(s) 815, 1278, 1850, 2069, 2073, 2417, 2421, 2570, 2914
HpyCH4III ACNGT 9 cut(s) 726, 818, 876, 1427, 1928, 2096, 2356, 2525, 2882
HpyCH4IV ACGT 5 cut(s) 588, 857, 1991, 2874, 3069
HpyF3I CTNAG 9 cut(s) 549, 810, 845, 1065, 1325, 1814, 1944, 2970, 3096
HpySE526I ACGT 5 cut(s) 588, 857, 1991, 2874, 3069
Hsp92II CATG 9 cut(s) 1214, 1955, 2070, 2351, 2443, 2548, 2567, 2579, 3055
HspAI GCGC 5 cut(s) 1219, 1919, 2082, 2227, 2627
Kpn2I TCCGGA 1 cut(s) 1296
KpnI GGTACC 1 cut(s) 1985
Ksp22I TGATCA 1 cut(s) 3001
KspAI GTTAAC 2 cut(s) 966, 1467
LweI GCATC 3 cut(s) 1701, 2695, 3142
MaeII ACGT 5 cut(s) 588, 857, 1991, 2874, 3069
MaeIII GTNAC 5 cut(s) 663, 1892, 2321, 2845, 2882
MfeI CAATTG 4 cut(s) 254, 346, 1776, 3143
MflI RGATCY 5 cut(s) 940, 1882, 2171, 2253, 2929
MhlI GDGCHC 5 cut(s) 83, 1336, 2347, 2421, 2736
MlsI TGGCCA 3 cut(s) 252, 344, 777
MluNI TGGCCA 3 cut(s) 252, 344, 777
MlyI GAGTC 5 cut(s) 286, 549, 562, 1012, 2714
MmeI TCCRAC 7 cut(s) 166, 333, 1258, 1622, 1626, 1689, 2628
Mox20I TGGCCA 3 cut(s) 252, 344, 777
MroI TCCGGA 1 cut(s) 1296
MscI TGGCCA 3 cut(s) 252, 344, 777
MslI CAYNNNNRTG 1 cut(s) 1019
Msp20I TGGCCA 3 cut(s) 252, 344, 777
MspA1I CMGCKG 1 cut(s) 1861
MspCI CTTAAG 2 cut(s) 1222, 3023
MspR9I CCNGG 5 cut(s) 151, 235, 337, 701, 1340
MunI CAATTG 4 cut(s) 254, 346, 1776, 3143
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 2 cut(s) 151, 701
MvnI CGCG 1 cut(s) 2084
NciI CCSGG 3 cut(s) 235, 337, 1340
NcoI CCATGG 3 cut(s) 1210, 2347, 3051
NdeI CATATG 2 cut(s) 1371, 2045
NlaIII CATG 9 cut(s) 1214, 1955, 2070, 2351, 2443, 2548, 2567, 2579, 3055
NmuCI GTSAC 2 cut(s) 663, 2321
NspV TTCGAA 1 cut(s) 1716
PaeR7I CTCGAG 1 cut(s) 605
PagI TCATGA 1 cut(s) 2563
PasI CCCWGGG 1 cut(s) 700
PceI AGGCCT 2 cut(s) 2136, 2366
PctI GAATGC 1 cut(s) 160
PflMI CCANNNNNTGG 2 cut(s) 214, 1778
PfoI TCCNGGA 1 cut(s) 1338
PinAI ACCGGT 2 cut(s) 968, 2775
PleI GAGTC 5 cut(s) 286, 548, 561, 1011, 2714
PpsI GAGTC 5 cut(s) 286, 548, 561, 1011, 2714
PpuMI RGGWCCY 2 cut(s) 260, 1835
PshAI GACNNNNGTC 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 441
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 2 cut(s) 83, 1336
Psp5II RGGWCCY 2 cut(s) 260, 1835
Psp6I CCWGG 2 cut(s) 149, 699
PspFI CCCAGC 1 cut(s) 1489
PspGI CCWGG 2 cut(s) 149, 699
PspPI GGNCC 7 cut(s) 260, 433, 1599, 1835, 1976, 2235, 2270
PspPPI RGGWCCY 2 cut(s) 260, 1835
PstI CTGCAG 2 cut(s) 1083, 1849
PsuI RGATCY 5 cut(s) 940, 1882, 2171, 2253, 2929
PvuII CAGCTG 1 cut(s) 1861
RseI CAYNNNNRTG 1 cut(s) 1019
SacI GAGCTC 2 cut(s) 83, 1336
Sau96I GGNCC 7 cut(s) 260, 433, 1599, 1835, 1976, 2235, 2270
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 5 cut(s) 286, 549, 562, 1012, 2714
ScrFI CCNGG 5 cut(s) 151, 235, 337, 701, 1340
SduI GDGCHC 5 cut(s) 83, 1336, 2347, 2421, 2736
SfaNI GCATC 3 cut(s) 1701, 2695, 3142
SfcI CTRYAG 6 cut(s) 123, 872, 1079, 1733, 1845, 2304
Sfr274I CTCGAG 1 cut(s) 605
SfuI TTCGAA 1 cut(s) 1716
SinI GGWCC 5 cut(s) 260, 433, 1599, 1835, 1976
SlaI CTCGAG 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 1019
SseBI AGGCCT 2 cut(s) 2136, 2366
SsiI CCGC 6 cut(s) 1481, 1510, 1597, 2084, 2414, 3155
SspI AATATT 1 cut(s) 1087
SstI GAGCTC 2 cut(s) 83, 1336
StuI AGGCCT 2 cut(s) 2136, 2366
StyD4I CCNGG 5 cut(s) 149, 233, 335, 699, 1338
StyI CCWWGG 6 cut(s) 1210, 2137, 2347, 2370, 2461, 3051
TaaI ACNGT 9 cut(s) 726, 818, 876, 1427, 1928, 2096, 2356, 2525, 2882
TaiI ACGT 5 cut(s) 591, 860, 1994, 2877, 3072
TatI WGTACW 4 cut(s) 350, 363, 1023, 3092
TscAI CASTG 5 cut(s) 122, 1189, 1333, 1690, 2982
TseFI GTSAC 2 cut(s) 663, 2321
Tsp45I GTSAC 2 cut(s) 663, 2321
TspDTI ATGAA 6 cut(s) 1940, 2012, 2055, 2552, 2592, 2720
TspGWI ACGGA 3 cut(s) 1330, 1614, 3005
TspRI CASTG 5 cut(s) 122, 1189, 1333, 1690, 2982
Van91I CCANNNNNTGG 2 cut(s) 214, 1778
Vha464I CTTAAG 2 cut(s) 1222, 3023
VpaK11BI GGWCC 5 cut(s) 260, 433, 1599, 1835, 1976
VspI ATTAAT 1 cut(s) 441
XagI CCTNNNNNAGG 1 cut(s) 3026
XapI RAATTY 7 cut(s) 19, 243, 490, 643, 903, 1343, 2451
XcmI CCANNNNNNNNNTGG 3 cut(s) 987, 2601, 2806
XhoI CTCGAG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 881
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.