Rroxscaffold_2G00097340
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011195
Physical Location & Seq
Forward (+)
18570367 .. 18574088
3722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00097340.1

Sequence Viewer

Length: 3324 bp
ATGCAGCTTTCTCTGTGCCATTTCCTTTTCTTGTTCTTGTGCTTCCCTGTTTCCATAACCAGTATAACTAGTTTGAACTCTGATGGGCTTGCTTTACTGTCCATCTTAAACCACTGGACTTTTGTCCCCAAAACCATTTCCTCAAGCTGGAATCTTTCTGATTCCACACCATGCAAATGGGTAGGTATAGAATGTGACCCTGCCCACAATGTTTTTGCCTTGAATGTCTCTAGTTATGGAATTTCTGGTCAGTTAGGACCTGAAATTGGGAACTTGAGTCACTTACAGACTCTTGTTTTGAGTTTCAATAATTTTTCTGGCCAAATTCCCATGGAGTTGGCCAATTGTAGACTTCTTGAGACCTTGGATTTGTCTGGAAATGGGTTCAGTGGAAAATTTCTTGATAACTTGCAAAAGCTGAAGGGTTTACAGTACTTGAGCTTGAATGATAATTTTTTGGTTGGGCAGATTCCTGAGTCCTTGTTTACAATTCCAAACTTAGCCAAGGTGTATTTGTACAATAACAGTTTGAATGGTTCAATCCCTGGAAATGTTGGTTCATTGAGTGAGCTTGTGCATTTGTATTTGTACAATAATGAGTTGTCGGGGGCGACTCCTTCATCCATTGGAAACTGTAGTAAATTGGAGGAATTGAATTTGAATTATAACCAGTTGAGTGGAGTATTGCCCGAGAGTCTGAACAACCTTAAGAACCTGTCCTATCTACAAGTCAGTAAGAACAGTCTTGAGGGTACCATTTCTTTTGGTTCAGAAAATTGCAAGAATTTGGTTTCCTTGGATTTATCATCCAATCAGTTTAGTGGAGTACTTCCACCAGCCCTTGCCAATTGTACTGGTTTAAGGGAATTTTCGGCTGCAGTTAACAATTTAGTTGGTCCAATACCGTCTTCCTTTGGCCTACTAGATAAGCTACAACTTCTATACCTTCCTGAGAATCAGCTGTCTGGAAAAATACCTCCAGAACTTGGCAAGTGTAAGTCCTTGACCGGACTACAATTGTACACAAACCAACTAGTTGGAGAAATTCCCGATGAGTTGGGAATGTTAACCAACTTGCAGGACCTTCAATTATTTCAAAACAACTTAACTGGTGAAATTCCAGTTAGCATATGGAAGATTCAAACTCTTCGATATGTCCATCTATACAAGAATAACCTCACTGGGGAACTGCCTCTGGAGATGACTGAGCTCAAGCAACTGGAAAACATCACATTATACAACAACCAGTTTTATGGAGTTGTACCTCGAGCTTTGGGGATTAACAGCAGTTTAGTTCAGTTAGATTTTATGTATAATATGTTCACTGGTAGCATCCCTCCAAATCTTTGCCTTGGAAAGAAGCTAAGGGTATTGAATATGGGGTTCAATCAATTTGAAGGTACCATACCTTCTGATGTCGGAAGTTGTTCAACTCTTTGGAGGTTGAGACTCGGACAGAATAACTTGACCGGAGTACTTCCGGAATTTGCAAAAAATTCTGACCTTTCATATATGGACATCAGCAGCAATGAGATTAGTGGAGAAATTCCATCAAGCTTGGGAAACTGTAGCAGCCTCACCTCCATCAACTTGGCCATGAATAAGTTAACAGGAGTTATACCAGAGGAGCTAGGGTACCTTGCAGATCTCCAAAAGTTGGATCTTTCCCACAACAGTTTGATGGGTGCTTTGCCTTCTAGCCTATCCAATTGTACCAAAATGGAGGAGTTTGATGTGGGGTCCAACTTCTTGAATGGCTCCATTCCTTTGAGTTTGAGTAGTTGGACAGGTTTATCTAAATTGGTATTAAGTGAAAACAGGTTTACTGGCGGCATTCCACCCTTCTTGTCAGAATTTGAAAAGCTTTCGGAGCTACAACTTGGTGGAAATTTGTTGGGAGGTGTGATTCCATCGTCAGTTGGAGCACTGCAGAATTTGTTTTATGCTCTGAACCTTAGCAATAATGCATTGACGGGTGTGGTTCCTTCAGAGCTTGGAAAGTTGACAACGCTACAGCGACTAGATTTATCTCATAACAACCTGACAGGGACTTTGAGAGCTCTTGATGGGATGAATTCATTGATTGAGGTTAATGTTTCATACAATAGCTTCACAGGTGCAGTTCCAGAAACATTGATGAACCTGTTGAACTCATCCTCATTTTCCTTTTCCAGCAATCCCTCCCTATGTGTCAGTTACCTTCCATCATGTGGCTTCACTTGTGCCGGAAACAACACTATTAAGCTCTGTAACAGTCAATCAAGCAAGCAGAAAGGACTTGGTAAAGTGGAAATTGCATTTATAGCCCTAGGATCATCGATACTAGTTGTTTCTCTGCTGTATCTACTGGTGTATTTGTTTTGCTTGCACAAAAGGACTAAGCAGGAGGTTGGGGTCTCAGCTCGAGAGGGTCCATCTTCCTTACTCTCCAAAGTCATGGAGGCTACAGAGAACCTTAGTGATAGATATATCATTGGGAGAGGAGCCCATGGAACTGTTTACAGAGCTTCTTTGGATGAAGACAAAGATTTTGCTGTCAAGAAGCTTGTATTTGAAGGGCATGAAGGAAGGCGTTTGAGCATGATTAGAGAACTTCAAACCCTTGGGAAGATCAGGCACCGGAATCTGGTTAAATTGGAAGACTTCTGGTTAAGAAAGGACTATGGCTTAATCTTGTATAGGTACATGCAAAATGGGAGCCTTCATGATGTTTTGCACGAAATTAGTCCCCCACTAATTCTGGAGTGGAGTGTCCGCTACAAGATAGCGCTGGGAACTGCATACGGCTTAGAATATCTCCATTATGATTGTGATCCCCCAATAGTGCATCGAGATATCAAGCCAATGAACATTCTGTTAGACTCTGATATGGAAGCTCATATTGCTGATTTTGGAATTGCTAAACTCTTGGATCAGTCTACTCCTTCAACGGTGTCCATCTCAGTTGTTGGTACAATCGGATATATCGCACCTGAAAATGCATTCAGAACAGCAAACAGTGTGGAATCTGATGTGTACAGTTATGGGGTTGTTTTACTTGAGCTGATAACTAGAAAGAAGGCATTGGATCCATCATTTATGGAGCAAACTGACATTGTAGGATGGGTTAGATCAGTGTGGAGCGACACAGAAAAAATTGATAGGATTGTTGATTCAAGCCTTGTGGAGGAACTTCTGGACTCAGATATCAGGGAACAAATTGTTGATGTGCTTCTTGTGGCTTTCAGCTGTACTCATAATGATCCTAAGAAGCGACCCACGATGAGAGATGTTATCAAGCAATTATTAGATGTAAATTCCCCAATGAGGAGCACAAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1107

Amino Acids

121.71

Weight (kDa)

5.36

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 66 1.8e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 101 - 254 3.3e-12 Leucine-rich repeat region
LRR_8 PF13855 190 - 249 1.5e-08 Leucine rich repeat
LRR_8 PF13855 479 - 536 9.8e-06 Leucine rich repeat
LRR_8 PF13855 502 - 560 4.7e-08 Leucine rich repeat
LRR_8 PF13855 621 - 681 6.4e-08 Leucine rich repeat
Pkinase PF00069 824 - 1094 1.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 824 - 1095 6e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
Acc65I GGTACC 3 cut(s) 752, 1400, 1635
AccB1I GGYRCC 4 cut(s) 752, 1400, 1635, 2616
AccB7I CCANNNNNTGG 3 cut(s) 986, 1657, 2210
AccI GTMKAC 2 cut(s) 349, 2918
AccIII TCCGGA 1 cut(s) 1480
AciI CCGC 2 cut(s) 1830, 2755
AclWI GGATC 7 cut(s) 1668, 2320, 2807, 2919, 3062, 3075, 3236
AcoI YGGCCR 3 cut(s) 319, 339, 1593
AcuI CTGAAG 2 cut(s) 440, 1971
AfeI AGCGCT 1 cut(s) 2769
AfiI CCNNNNNNNGG 9 cut(s) 147, 266, 986, 1183, 1657, 2210, 2626, 3166, 3306
AflII CTTAAG 1 cut(s) 707
AhlI ACTAGT 3 cut(s) 68, 1033, 2323
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
Alw21I GWGCWC 4 cut(s) 1212, 1927, 2062, 3314
Alw26I GTCTC 4 cut(s) 232, 353, 1441, 2401
AlwI GGATC 7 cut(s) 1668, 2320, 2807, 2919, 3062, 3075, 3236
Ama87I CYCGRG 3 cut(s) 689, 1266, 2403
Aor13HI TCCGGA 1 cut(s) 1480
Aor51HI AGCGCT 1 cut(s) 2769
AoxI GGCC 4 cut(s) 319, 339, 916, 1593
ApeKI GCWGC 4 cut(s) 4, 875, 1524, 1572
ArsI GACNNNNNNTTYG 8 cut(s) 280, 312, 352, 384, 2035, 2067, 2513, 2545
Asp700I GAANNNNTTC 1 cut(s) 1426
Asp718I GGTACC 3 cut(s) 752, 1400, 1635
AspA2I CCTAGG 1 cut(s) 2308
AspLEI GCGC 1 cut(s) 2770
AspS9I GGNCC 5 cut(s) 257, 896, 1081, 1740, 2411
AsuHPI GGTGA 2 cut(s) 1124, 1570
AvaI CYCGRG 3 cut(s) 689, 1266, 2403
AvaII GGWCC 5 cut(s) 257, 896, 1081, 1740, 2411
AvrII CCTAGG 1 cut(s) 2308
BalI TGGCCA 3 cut(s) 321, 341, 1595
BamHI GGATCC 1 cut(s) 3067
BanI GGYRCC 4 cut(s) 752, 1400, 1635, 2616
BanII GRGCYC 3 cut(s) 1212, 2062, 2488
BarI GAAGNNNNNNTAC 4 cut(s) 1389, 1393, 1421, 1425
BbsI GAAGAC 3 cut(s) 900, 2526, 2646
Bbv12I GWGCWC 4 cut(s) 1212, 1927, 2062, 3314
BbvI GCAGC 4 cut(s) 16, 862, 1536, 1584
BceAI ACGGC 1 cut(s) 2800
BciT130I CCWGG 1 cut(s) 546
BcoDI GTCTC 4 cut(s) 232, 353, 1441, 2401
BcuI ACTAGT 3 cut(s) 68, 1033, 2323
BfmI CTRYAG 6 cut(s) 634, 876, 1567, 1928, 2012, 2445
BfoI RGCGCY 1 cut(s) 2771
BfrI CTTAAG 1 cut(s) 707
BglII AGATCT 1 cut(s) 1645
BisI GCNGC 5 cut(s) 5, 876, 1525, 1573, 1831
BlnI CCTAGG 1 cut(s) 2308
BlsI GCNGC 5 cut(s) 6, 877, 1526, 1574, 1832
BmcAI AGTACT 3 cut(s) 434, 828, 1476
Bme1390I CCNGG 1 cut(s) 546
Bme18I GGWCC 5 cut(s) 257, 896, 1081, 1740, 2411
BmeT110I CYCGRG 3 cut(s) 689, 1266, 2403
BmgT120I GGNCC 5 cut(s) 257, 896, 1081, 1740, 2411
BmrFI CCNGG 1 cut(s) 546
BmrI ACTGGG 1 cut(s) 1191
BmsI GCATC 2 cut(s) 1341, 2836
BmuI ACTGGG 1 cut(s) 1191
BoxI GACNNNNGTC 1 cut(s) 122
BpiI GAAGAC 3 cut(s) 900, 2526, 2646
BpmI CTGGAG 3 cut(s) 963, 1217, 2762
Bpu10I CCTNAGC 2 cut(s) 1364, 1955
BpuEI CTTGAG 7 cut(s) 127, 295, 377, 457, 767, 1196, 3059
Bsa29I ATCGAT 1 cut(s) 2318
BsaBI GATNNNNATC 1 cut(s) 2811
BsaI GGTCTC 2 cut(s) 353, 2401
BsaJI CCNNGG 9 cut(s) 330, 363, 504, 544, 795, 1351, 2308, 2488, 2602
BsaWI WCCGGW 4 cut(s) 1007, 1469, 1480, 2619
BsaXI ACNNNNNCTCC 2 cut(s) 1619, 1649
Bsc4I CCNNNNNNNGG 9 cut(s) 147, 266, 986, 1183, 1657, 2210, 2626, 3166, 3306
Bse3DI GCAATG 1 cut(s) 1534
Bse8I GATNNNNATC 1 cut(s) 2811
BseAI TCCGGA 1 cut(s) 1480
BseBI CCWGG 1 cut(s) 546
BseCI ATCGAT 1 cut(s) 2318
BseDI CCNNGG 9 cut(s) 330, 363, 504, 544, 795, 1351, 2308, 2488, 2602
BseGI GGATG 7 cut(s) 620, 806, 1332, 2076, 2153, 2521, 3107
BseJI GATNNNNATC 1 cut(s) 2811
BseLI CCNNNNNNNGG 9 cut(s) 147, 266, 986, 1183, 1657, 2210, 2626, 3166, 3306
BseMI GCAATG 1 cut(s) 1534
BseMII CTCAG 6 cut(s) 465, 942, 1197, 2412, 2955, 3195
BseRI GAGGAG 4 cut(s) 1640, 1739, 2496, 3322
BseXI GCAGC 4 cut(s) 16, 862, 1536, 1584
BseYI CCCAGC 1 cut(s) 2770
BsgI GTGCAG 1 cut(s) 2139
BshFI GGCC 4 cut(s) 321, 341, 918, 1595
BshNI GGYRCC 4 cut(s) 752, 1400, 1635, 2616
BshVI ATCGAT 1 cut(s) 2318
BsiHKAI GWGCWC 4 cut(s) 1212, 1927, 2062, 3314
BsiHKCI CYCGRG 3 cut(s) 689, 1266, 2403
BsiSI CCGG 5 cut(s) 1008, 1470, 1481, 2226, 2620
BslFI GGGAC 3 cut(s) 110, 2062, 2712
BslI CCNNNNNNNGG 9 cut(s) 147, 266, 986, 1183, 1657, 2210, 2626, 3166, 3306
BsmAI GTCTC 4 cut(s) 232, 353, 1441, 2401
BsmFI GGGAC 3 cut(s) 110, 2062, 2712
BsmI GAATGC 2 cut(s) 1833, 2981
BsnI GGCC 4 cut(s) 321, 341, 918, 1595
Bso31I GGTCTC 2 cut(s) 353, 2401
BsoBI CYCGRG 3 cut(s) 689, 1266, 2403
Bsp1286I GDGCHC 5 cut(s) 1212, 1927, 2062, 2488, 3314
Bsp13I TCCGGA 1 cut(s) 1480
Bsp1407I TGTACA 4 cut(s) 516, 588, 1020, 3015
Bsp143I GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
Bsp19I CCATGG 2 cut(s) 330, 2488
BspACI CCGC 2 cut(s) 1830, 2755
BspANI GGCC 4 cut(s) 321, 341, 918, 1595
BspCNI CTCAG 6 cut(s) 466, 943, 1198, 2411, 2954, 3194
BspDI ATCGAT 1 cut(s) 2318
BspEI TCCGGA 1 cut(s) 1480
BspHI TCATGA 1 cut(s) 2704
BspMAI CTGCAG 2 cut(s) 880, 1932
BspPI GGATC 7 cut(s) 1668, 2320, 2807, 2919, 3062, 3075, 3236
BspT107I GGYRCC 4 cut(s) 752, 1400, 1635, 2616
BspTI CTTAAG 1 cut(s) 707
BspTNI GGTCTC 2 cut(s) 353, 2401
BsrDI GCAATG 1 cut(s) 1534
BsrGI TGTACA 4 cut(s) 516, 588, 1020, 3015
BssECI CCNNGG 9 cut(s) 330, 363, 504, 544, 795, 1351, 2308, 2488, 2602
BssMI GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
BssT1I CCWWGG 8 cut(s) 330, 363, 504, 795, 1351, 2308, 2488, 2602
Bst2UI CCWGG 1 cut(s) 546
Bst6I CTCTTC 1 cut(s) 1152
BstAFI CTTAAG 1 cut(s) 707
BstAUI TGTACA 4 cut(s) 516, 588, 1020, 3015
BstC8I GCNNGC 3 cut(s) 90, 2267, 2366
BstDSI CCRYGG 2 cut(s) 330, 2488
BstENI CCTNNNNNAGG 1 cut(s) 3164
BstF5I GGATG 7 cut(s) 620, 806, 1332, 2076, 2153, 2521, 3107
BstH2I RGCGCY 1 cut(s) 2771
BstHHI GCGC 1 cut(s) 2770
BstKTI GATC 9 cut(s) 1648, 1663, 2315, 2613, 2815, 2914, 3070, 3113, 3244
BstMAI GTCTC 4 cut(s) 232, 353, 1441, 2401
BstMBI GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
BstMWI GCNNNNNNNGC 3 cut(s) 1870, 2303, 2882
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 2689
BstPAI GACNNNNGTC 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 544
BstSFI CTRYAG 6 cut(s) 634, 876, 1567, 1928, 2012, 2445
BstV1I GCAGC 4 cut(s) 16, 862, 1536, 1584
BstV2I GAAGAC 3 cut(s) 900, 2526, 2646
BstX2I RGATCY 3 cut(s) 1645, 1660, 3067
BstXI CCANNNNNNTGG 7 cut(s) 177, 337, 677, 1037, 1253, 1591, 2437
BstYI RGATCY 3 cut(s) 1645, 1660, 3067
Bsu15I ATCGAT 1 cut(s) 2318
BsuRI GGCC 4 cut(s) 321, 341, 918, 1595
BsuTUI ATCGAT 1 cut(s) 2318
BtgI CCRYGG 2 cut(s) 330, 2488
BtsCI GGATG 7 cut(s) 620, 806, 1332, 2076, 2153, 2521, 3107
BtsI GCAGTG 1 cut(s) 1925
BtsIMutI CAGTG 7 cut(s) 112, 394, 1179, 1323, 1925, 3004, 3120
Cac8I GCNNGC 3 cut(s) 90, 2267, 2366
CciI TCATGA 1 cut(s) 2704
CfoI GCGC 1 cut(s) 2770
Cfr13I GGNCC 5 cut(s) 257, 896, 1081, 1740, 2411
ClaI ATCGAT 1 cut(s) 2318
CspCI CAANNNNNGTGG 6 cut(s) 1827, 1862, 2982, 3017, 3144, 3179
DpnI GATC 9 cut(s) 1647, 1662, 2314, 2612, 2814, 2913, 3069, 3112, 3243
DpnII GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
EaeI YGGCCR 3 cut(s) 319, 339, 1593
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
Ecl136II GAGCTC 2 cut(s) 1210, 2060
Eco130I CCWWGG 8 cut(s) 330, 363, 504, 795, 1351, 2308, 2488, 2602
Eco24I GRGCYC 3 cut(s) 1212, 2062, 2488
Eco31I GGTCTC 2 cut(s) 353, 2401
Eco32I GATATC 2 cut(s) 2836, 3187
Eco47I GGWCC 5 cut(s) 257, 896, 1081, 1740, 2411
Eco47III AGCGCT 1 cut(s) 2769
Eco53kI GAGCTC 2 cut(s) 1210, 2060
Eco57I CTGAAG 2 cut(s) 440, 1971
Eco88I CYCGRG 3 cut(s) 689, 1266, 2403
EcoICRI GAGCTC 2 cut(s) 1210, 2060
EcoNI CCTNNNNNAGG 1 cut(s) 3164
EcoO109I RGGNCCY 2 cut(s) 257, 1081
EcoRI GAATTC 1 cut(s) 2074
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 2 cut(s) 2836, 3187
EcoT14I CCWWGG 8 cut(s) 330, 363, 504, 795, 1351, 2308, 2488, 2602
EcoT22I ATGCAT 2 cut(s) 1969, 2983
EcoT38I GRGCYC 3 cut(s) 1212, 2062, 2488
ErhI CCWWGG 8 cut(s) 330, 363, 504, 795, 1351, 2308, 2488, 2602
FaqI GGGAC 3 cut(s) 110, 2062, 2712
FauNDI CATATG 1 cut(s) 1130
FblI GTMKAC 2 cut(s) 349, 2918
Fnu4HI GCNGC 5 cut(s) 5, 876, 1525, 1573, 1831
FokI GGATG 7 cut(s) 607, 793, 1319, 2083, 2140, 2528, 3114
FriOI GRGCYC 3 cut(s) 1212, 2062, 2488
Fsp4HI GCNGC 5 cut(s) 5, 876, 1525, 1573, 1831
GlaI GCGC 1 cut(s) 2769
GluI GCNGC 5 cut(s) 5, 876, 1525, 1573, 1831
GsaI CCCAGC 1 cut(s) 2774
GsuI CTGGAG 3 cut(s) 963, 1217, 2762
HaeII RGCGCY 1 cut(s) 2771
HaeIII GGCC 4 cut(s) 321, 341, 918, 1595
HapII CCGG 5 cut(s) 1008, 1470, 1481, 2226, 2620
HhaI GCGC 1 cut(s) 2770
Hin6I GCGC 1 cut(s) 2768
HinP1I GCGC 1 cut(s) 2768
HincII GTYRAC 4 cut(s) 883, 1068, 1608, 2004
HindII GTYRAC 4 cut(s) 883, 1068, 1608, 2004
HindIII AAGCTT 3 cut(s) 1555, 1862, 2543
HpaI GTTAAC 3 cut(s) 883, 1068, 1608
HpaII CCGG 5 cut(s) 1008, 1470, 1481, 2226, 2620
HphI GGTGA 2 cut(s) 1124, 1570
HpyF10VI GCNNNNNNNGC 3 cut(s) 1870, 2303, 2882
HspAI GCGC 1 cut(s) 2768
Kpn2I TCCGGA 1 cut(s) 1480
KpnI GGTACC 3 cut(s) 756, 1404, 1639
KspAI GTTAAC 3 cut(s) 883, 1068, 1608
Kzo9I GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
LmnI GCTCC 9 cut(s) 1627, 1763, 1870, 1922, 2483, 2697, 3082, 3120, 3309
Lsp1109I GCAGC 4 cut(s) 16, 862, 1536, 1584
LweI GCATC 2 cut(s) 1341, 2836
MaeIII GTNAC 4 cut(s) 194, 278, 2195, 2249
MalI GATC 9 cut(s) 1647, 1662, 2314, 2612, 2814, 2913, 3069, 3112, 3243
MboI GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
MboII GAAGA 7 cut(s) 900, 1139, 1147, 2409, 2531, 2620, 2651
MfeI CAATTG 4 cut(s) 343, 847, 1016, 1708
MflI RGATCY 3 cut(s) 1645, 1660, 3067
MhlI GDGCHC 5 cut(s) 1212, 1927, 2062, 2488, 3314
MlsI TGGCCA 3 cut(s) 321, 341, 1595
MluNI TGGCCA 3 cut(s) 321, 341, 1595
MlyI GAGTC 8 cut(s) 283, 286, 485, 607, 703, 1443, 2855, 3173
MmeI TCCRAC 6 cut(s) 1018, 1399, 1638, 1763, 1767, 1900
Mox20I TGGCCA 3 cut(s) 321, 341, 1595
Mph1103I ATGCAT 2 cut(s) 1969, 2983
MroI TCCGGA 1 cut(s) 1480
MroXI GAANNNNTTC 1 cut(s) 1426
MscI TGGCCA 3 cut(s) 321, 341, 1595
MslI CAYNNNNRTG 1 cut(s) 175
Msp20I TGGCCA 3 cut(s) 321, 341, 1595
MspA1I CMGCKG 2 cut(s) 961, 3228
MspCI CTTAAG 1 cut(s) 707
MspI CCGG 5 cut(s) 1008, 1470, 1481, 2226, 2620
MspR9I CCNGG 1 cut(s) 546
MunI CAATTG 4 cut(s) 343, 847, 1016, 1708
Mva1269I GAATGC 2 cut(s) 1833, 2981
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 3 cut(s) 1870, 2303, 2882
NcoI CCATGG 2 cut(s) 330, 2488
NdeI CATATG 1 cut(s) 1130
NdeII GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
NmuCI GTSAC 2 cut(s) 194, 278
NsiI ATGCAT 2 cut(s) 1969, 2983
NspI RCATGY 1 cut(s) 2689
PaeR7I CTCGAG 2 cut(s) 1266, 2403
PagI TCATGA 1 cut(s) 2704
PctI GAATGC 2 cut(s) 1833, 2981
PdmI GAANNNNTTC 1 cut(s) 1426
PfeI GAWTC 9 cut(s) 151, 161, 469, 955, 1138, 1906, 2623, 3005, 3152
PflMI CCANNNNNTGG 3 cut(s) 986, 1657, 2210
PkrI GCNGC 5 cut(s) 6, 877, 1526, 1574, 1832
PleI GAGTC 8 cut(s) 283, 285, 484, 607, 702, 1443, 2855, 3173
PpsI GAGTC 8 cut(s) 283, 285, 484, 607, 702, 1443, 2855, 3173
PpuMI RGGWCCY 2 cut(s) 257, 1081
PshAI GACNNNNGTC 1 cut(s) 122
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 2 cut(s) 1212, 2062
Psp5II RGGWCCY 2 cut(s) 257, 1081
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 2770
PspGI CCWGG 1 cut(s) 544
PspPI GGNCC 5 cut(s) 257, 896, 1081, 1740, 2411
PspPPI RGGWCCY 2 cut(s) 257, 1081
PspXI VCTCGAGB 1 cut(s) 1266
PsrI GAACNNNNNNTAC 2 cut(s) 1304, 1336
PstI CTGCAG 2 cut(s) 880, 1932
PsuI RGATCY 3 cut(s) 1645, 1660, 3067
PvuII CAGCTG 2 cut(s) 961, 3228
RseI CAYNNNNRTG 1 cut(s) 175
SacI GAGCTC 2 cut(s) 1212, 2062
SatI GCNGC 5 cut(s) 5, 876, 1525, 1573, 1831
Sau3AI GATC 9 cut(s) 1645, 1660, 2312, 2610, 2812, 2911, 3067, 3110, 3241
Sau96I GGNCC 5 cut(s) 257, 896, 1081, 1740, 2411
ScaI AGTACT 3 cut(s) 434, 828, 1476
SchI GAGTC 8 cut(s) 283, 286, 485, 607, 703, 1443, 2855, 3173
ScrFI CCNGG 1 cut(s) 546
SduI GDGCHC 5 cut(s) 1212, 1927, 2062, 2488, 3314
SfaNI GCATC 2 cut(s) 1341, 2836
SfcI CTRYAG 6 cut(s) 634, 876, 1567, 1928, 2012, 2445
Sfr274I CTCGAG 2 cut(s) 1266, 2403
SinI GGWCC 5 cut(s) 257, 896, 1081, 1740, 2411
SlaI CTCGAG 2 cut(s) 1266, 2403
SmiMI CAYNNNNRTG 1 cut(s) 175
SpeI ACTAGT 3 cut(s) 68, 1033, 2323
SsiI CCGC 2 cut(s) 1830, 2755
SstI GAGCTC 2 cut(s) 1212, 2062
StyD4I CCNGG 1 cut(s) 544
StyI CCWWGG 8 cut(s) 330, 363, 504, 795, 1351, 2308, 2488, 2602
TaqI TCGA 5 cut(s) 1150, 1267, 2318, 2404, 2830
TatI WGTACW 9 cut(s) 432, 516, 588, 826, 851, 1020, 1474, 3015, 3230
TauI GCSGC 1 cut(s) 1833
TfiI GAWTC 9 cut(s) 151, 161, 469, 955, 1138, 1906, 2623, 3005, 3152
TscAI CASTG 7 cut(s) 119, 394, 1186, 1330, 1932, 3004, 3120
TseFI GTSAC 2 cut(s) 194, 278
TseI GCWGC 4 cut(s) 4, 875, 1524, 1572
Tsp45I GTSAC 2 cut(s) 194, 278
TspRI CASTG 7 cut(s) 119, 394, 1186, 1330, 1932, 3004, 3120
Van91I CCANNNNNTGG 3 cut(s) 986, 1657, 2210
Vha464I CTTAAG 1 cut(s) 707
VpaK11BI GGWCC 5 cut(s) 257, 896, 1081, 1740, 2411
XagI CCTNNNNNAGG 1 cut(s) 3164
XceI RCATGY 1 cut(s) 2689
XcmI CCANNNNNNNNNTGG 1 cut(s) 1128
XhoI CTCGAG 2 cut(s) 1266, 2403
XmaJI CCTAGG 1 cut(s) 2308
XmiI GTMKAC 2 cut(s) 349, 2918
XmnI GAANNNNTTC 1 cut(s) 1426
ZrmI AGTACT 3 cut(s) 434, 828, 1476
Zsp2I ATGCAT 2 cut(s) 1969, 2983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.