Rmu_ssc0000020.1_g000002
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000020.1
OG0011195
Physical Location & Seq
Forward (+)
11770 .. 13669
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000020.1_g000002.1.cds

Sequence Viewer

Length: 1812 bp
atggacatcagcagcaatgagattagtggagaaattccatcaagcttgggaaagtgtagcagcctcacctccatcaacttggccatgaataagttaacaggagttataccagaggagctaggataccttgcagatcttcatattttggatctttcccacaacagtttggtgggtgctttgccttctagcctatccaattgcaccaaaatgctggagtttgatgtggggtccaacttcttgaatggctccattcctttgagtttgagtagttggacaggtttatctaaattggtattaagtgagaacaggtttactggcggcattccaaccttcttgtcagaatttaaagagctttcggagctacaacttggtggaaatttgttgggaggtgtgattccatcgtcagttggagcactgcagaatttgttttatgctctgaaccttagcaataatgcattgacaggtgtggttccttcagagcttggaaagttgacaatgctacagcgactagatttatctcataacaatctgacggggactttgagggctcttgatgggatgatttcattgattgaggttaatgtttcatacaatagcttcacaggtgcagtaccagaaacattgatgaacctgttaaactcatcctcattttccttttccagcaatccctccctatgtgtcagttaccttccatcatgtggcttcacttgtgctggaaacaacactattaagctctgtaatagtcaatcaagcaagcagaaaggacttagtaaagtggaaattgcatttatagccctaggatcatcgatactagttgtttctctgctgtatctactggtgtatttgttttgcttgcacaaaaggactacgcaggaggttggggtctcagctcgagagggtccatcttccttactctccaaagtcatggaggctacagagaaccttagtgatagatatatcattgggagtggagcccatggaactgtttatcgagcttctttggatgaagacaaagattttgctgtcaagaagcttgtatttgaagggcatgaaggaaggcgtttgagcatgattagagaacttcaaacccttgggaagatcaggcaccggaatctggttaaattggaagacttctggttaagaaaggactatggcttaatcttgtataggtacatgcaaaatgggagccttcatgatgttttgcacgaaattagccccccactaattctggagtggagtgtccgctacaagatagcactgggaactgcatacggcttagaatatctccattatgattgtgatcccccaatagtgcatcgagatatcaagccaatgaacattctgttagactctgatatggaagctcatattgctgattttggaattgctaaactcttggatcagtctactccttcaacggtgtccatctcagttgttggtacaaccggatatatcgcaccagaaaatgcattcagaacagcaagcagtgtggaatctgatgtgtacagttatggggttgttttacttgagctgataactagaaagaaggcattggatccatcatttatggagcaaactgacattgcaggatgggttaaatccgtgtggggcgacacagaaaaaattgataggattgttgattcaagccttgtgggggaacttctggactcagatatcagggaacaaattgttgatgtgcttcttgtggctttcagctgtactcataatgatcctaagaagcgacccacgatgagagatgttatcaagcaattattagatgtaaattccccaatgaggagcacaaaattctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

603

Amino Acids

66.1

Weight (kDa)

5.54

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1104
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 1406
AciI CCGC 2 cut(s) 318, 1243
AclWI GGATC 7 cut(s) 156, 808, 1295, 1407, 1550, 1563, 1724
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 6 cut(s) 33, 341, 376, 421, 1783, 1805
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 5 cut(s) 612, 1172, 1441, 1505, 1720
AfiI CCNNNNNNNGG 4 cut(s) 698, 1114, 1654, 1794
AgsI TTSAA 5 cut(s) 241, 1043, 1085, 1416, 1644
AhlI ACTAGT 1 cut(s) 811
Alw21I GWGCWC 2 cut(s) 415, 1802
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 7 cut(s) 156, 808, 1295, 1407, 1550, 1563, 1724
Ama87I CYCGRG 1 cut(s) 891
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 12, 60
ApoI RAATTY 6 cut(s) 33, 341, 376, 421, 1783, 1805
ArsI GACNNNNNNTTYG 4 cut(s) 523, 555, 1001, 1033
AspA2I CCTAGG 1 cut(s) 796
AspS9I GGNCC 2 cut(s) 228, 899
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 891
AvaII GGWCC 2 cut(s) 228, 899
AvrII CCTAGG 1 cut(s) 796
BalI TGGCCA 1 cut(s) 83
BamHI GGATCC 1 cut(s) 1555
BanI GGYRCC 1 cut(s) 1104
BanII GRGCYC 2 cut(s) 550, 976
BbsI GAAGAC 2 cut(s) 1014, 1134
Bbv12I GWGCWC 2 cut(s) 415, 1802
BbvI GCAGC 2 cut(s) 24, 72
BccI CCATC 9 cut(s) 46, 80, 406, 548, 700, 910, 1433, 1567, 1584
BceAI ACGGC 1 cut(s) 1288
BciVI GTATCC 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 889
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 7 cut(s) 119, 186, 509, 797, 812, 1539, 1810
BfmI CTRYAG 3 cut(s) 416, 500, 933
BfuI GTATCC 1 cut(s) 116
BglII AGATCT 1 cut(s) 133
BisI GCNGC 3 cut(s) 13, 61, 319
BlnI CCTAGG 1 cut(s) 796
BlsI GCNGC 3 cut(s) 14, 62, 320
Bme18I GGWCC 2 cut(s) 228, 899
BmeT110I CYCGRG 1 cut(s) 891
BmgT120I GGNCC 2 cut(s) 228, 899
BmiI GGNNCC 8 cut(s) 229, 247, 471, 900, 973, 1106, 1187, 1557
BmrI ACTGGG 1 cut(s) 1268
BmsI GCATC 1 cut(s) 1324
BmuI ACTGGG 1 cut(s) 1268
BpiI GAAGAC 2 cut(s) 1014, 1134
BpmI CTGGAG 2 cut(s) 233, 1250
Bpu10I CCTNAGC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 1547
Bsa29I ATCGAT 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 1299
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 3 cut(s) 796, 976, 1090
BsaWI WCCGGW 2 cut(s) 1107, 1445
Bsc4I CCNNNNNNNGG 4 cut(s) 698, 1114, 1654, 1794
Bse1I ACTGG 3 cut(s) 319, 840, 1263
Bse3DI GCAATG 2 cut(s) 22, 1581
Bse8I GATNNNNATC 1 cut(s) 1299
BseCI ATCGAT 1 cut(s) 806
BseDI CCNNGG 3 cut(s) 796, 976, 1090
BseGI GGATG 4 cut(s) 564, 641, 1009, 1595
BseJI GATNNNNATC 1 cut(s) 1299
BseLI CCNNNNNNNGG 4 cut(s) 698, 1114, 1654, 1794
BseMI GCAATG 2 cut(s) 22, 1581
BseMII CTCAG 3 cut(s) 900, 1443, 1683
BseNI ACTGG 3 cut(s) 319, 840, 1263
BseRI GAGGAG 2 cut(s) 128, 1810
BseXI GCAGC 2 cut(s) 24, 72
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 1104
BshVI ATCGAT 1 cut(s) 806
BsiHKAI GWGCWC 2 cut(s) 415, 1802
BsiHKCI CYCGRG 1 cut(s) 891
BsiSI CCGG 2 cut(s) 1108, 1446
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 4 cut(s) 698, 1114, 1654, 1794
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 2 cut(s) 321, 1469
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 891
Bsp1286I GDGCHC 4 cut(s) 415, 550, 976, 1802
Bsp1407I TGTACA 1 cut(s) 1503
Bsp143I GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
Bsp19I CCATGG 1 cut(s) 976
BspACI CCGC 2 cut(s) 318, 1243
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 3 cut(s) 899, 1442, 1682
BspDI ATCGAT 1 cut(s) 806
BspHI TCATGA 1 cut(s) 1192
BspLI GGNNCC 8 cut(s) 229, 247, 471, 900, 973, 1106, 1187, 1557
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 7 cut(s) 156, 808, 1295, 1407, 1550, 1563, 1724
BspT107I GGYRCC 1 cut(s) 1104
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 22, 1581
BsrGI TGTACA 1 cut(s) 1503
BsrI ACTGG 3 cut(s) 319, 840, 1263
BssECI CCNNGG 3 cut(s) 796, 976, 1090
BssMI GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
BssT1I CCWWGG 3 cut(s) 796, 976, 1090
Bst4CI ACNGT 4 cut(s) 164, 985, 1420, 1508
BstAUI TGTACA 1 cut(s) 1503
BstC8I GCNNGC 3 cut(s) 755, 854, 1483
BstDEI CTNAG 8 cut(s) 443, 767, 886, 944, 1276, 1429, 1669, 1734
BstDSI CCRYGG 1 cut(s) 976
BstF5I GGATG 4 cut(s) 564, 641, 1009, 1595
BstKTI GATC 8 cut(s) 136, 151, 803, 1101, 1303, 1402, 1558, 1732
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
BstMWI GCNNNNNNNGC 3 cut(s) 358, 791, 1370
BstNSI RCATGY 1 cut(s) 1177
BstSFI CTRYAG 3 cut(s) 416, 500, 933
BstV1I GCAGC 2 cut(s) 24, 72
BstV2I GAAGAC 2 cut(s) 1014, 1134
BstX2I RGATCY 3 cut(s) 133, 148, 1555
BstXI CCANNNNNNTGG 3 cut(s) 79, 211, 925
BstYI RGATCY 3 cut(s) 133, 148, 1555
Bsu15I ATCGAT 1 cut(s) 806
BsuI GTATCC 1 cut(s) 116
BsuRI GGCC 1 cut(s) 83
BsuTUI ATCGAT 1 cut(s) 806
BtgI CCRYGG 1 cut(s) 976
BtsCI GGATG 4 cut(s) 564, 641, 1009, 1595
BtsI GCAGTG 2 cut(s) 413, 1492
BtsIMutI CAGTG 3 cut(s) 413, 1256, 1492
Cac8I GCNNGC 3 cut(s) 755, 854, 1483
CciI TCATGA 1 cut(s) 1192
Cfr13I GGNCC 2 cut(s) 228, 899
ClaI ATCGAT 1 cut(s) 806
Csp6I GTAC 5 cut(s) 611, 1171, 1440, 1504, 1719
CspCI CAANNNNNGTGG 4 cut(s) 1470, 1505, 1632, 1667
CviAII CATG 8 cut(s) 85, 696, 925, 977, 1049, 1069, 1174, 1193
CviQI GTAC 5 cut(s) 611, 1171, 1440, 1504, 1719
DdeI CTNAG 8 cut(s) 443, 767, 886, 944, 1276, 1429, 1669, 1734
DpnI GATC 8 cut(s) 135, 150, 802, 1100, 1302, 1401, 1557, 1731
DpnII GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
DraI TTTAAA 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 81
Eco130I CCWWGG 3 cut(s) 796, 976, 1090
Eco24I GRGCYC 2 cut(s) 550, 976
Eco31I GGTCTC 1 cut(s) 889
Eco32I GATATC 2 cut(s) 1324, 1675
Eco47I GGWCC 2 cut(s) 228, 899
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 891
EcoRV GATATC 2 cut(s) 1324, 1675
EcoT14I CCWWGG 3 cut(s) 796, 976, 1090
EcoT22I ATGCAT 2 cut(s) 457, 1471
EcoT38I GRGCYC 2 cut(s) 550, 976
ErhI CCWWGG 3 cut(s) 796, 976, 1090
FaeI CATG 8 cut(s) 88, 699, 928, 980, 1052, 1072, 1177, 1196
FaqI GGGAC 1 cut(s) 550
FatI CATG 8 cut(s) 84, 695, 924, 976, 1048, 1068, 1173, 1192
FblI GTMKAC 1 cut(s) 1406
Fnu4HI GCNGC 3 cut(s) 13, 61, 319
FokI GGATG 4 cut(s) 571, 628, 1016, 1602
FriOI GRGCYC 2 cut(s) 550, 976
Fsp4HI GCNGC 3 cut(s) 13, 61, 319
FspBI CTAG 7 cut(s) 119, 186, 509, 797, 812, 1539, 1810
GluI GCNGC 3 cut(s) 13, 61, 319
GsuI CTGGAG 2 cut(s) 233, 1250
HaeIII GGCC 1 cut(s) 83
HapII CCGG 2 cut(s) 1108, 1446
Hin1II CATG 8 cut(s) 88, 699, 928, 980, 1052, 1072, 1177, 1196
HincII GTYRAC 2 cut(s) 96, 492
HindII GTYRAC 2 cut(s) 96, 492
HindIII AAGCTT 2 cut(s) 43, 1031
HinfI GANTC 6 cut(s) 394, 1111, 1349, 1493, 1640, 1667
HpaI GTTAAC 1 cut(s) 96
HpaII CCGG 2 cut(s) 1108, 1446
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 5 cut(s) 96, 312, 492, 1407, 1504
Hpy188I TCNGA 9 cut(s) 340, 358, 438, 478, 531, 1354, 1475, 1498, 1672
Hpy188III TCNNGA 8 cut(s) 238, 551, 893, 1027, 1193, 1229, 1319, 1664
Hpy8I GTNNAC 5 cut(s) 96, 312, 492, 1407, 1504
HpyCH4III ACNGT 4 cut(s) 164, 985, 1420, 1508
HpyF10VI GCNNNNNNNGC 3 cut(s) 358, 791, 1370
HpyF3I CTNAG 8 cut(s) 443, 767, 886, 944, 1276, 1429, 1669, 1734
Hsp92II CATG 8 cut(s) 88, 699, 928, 980, 1052, 1072, 1177, 1196
KspAI GTTAAC 1 cut(s) 96
Kzo9I GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
LmnI GCTCC 8 cut(s) 115, 251, 358, 410, 971, 1185, 1570, 1797
Lsp1109I GCAGC 2 cut(s) 24, 72
LweI GCATC 1 cut(s) 1324
MaeI CTAG 7 cut(s) 119, 186, 509, 797, 812, 1539, 1810
MaeIII GTNAC 1 cut(s) 683
MalI GATC 8 cut(s) 135, 150, 802, 1100, 1302, 1401, 1557, 1731
MboI GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
MboII GAAGA 5 cut(s) 128, 897, 1019, 1108, 1139
MfeI CAATTG 1 cut(s) 196
MflI RGATCY 3 cut(s) 133, 148, 1555
MhlI GDGCHC 4 cut(s) 415, 550, 976, 1802
MlsI TGGCCA 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 2 cut(s) 1343, 1661
MmeI TCCRAC 4 cut(s) 251, 255, 350, 388
Mox20I TGGCCA 1 cut(s) 83
Mph1103I ATGCAT 2 cut(s) 457, 1471
MscI TGGCCA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 1716
MspI CCGG 2 cut(s) 1108, 1446
MunI CAATTG 1 cut(s) 196
Mva1269I GAATGC 2 cut(s) 321, 1469
MwoI GCNNNNNNNGC 3 cut(s) 358, 791, 1370
NcoI CCATGG 1 cut(s) 976
NdeII GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
NlaIII CATG 8 cut(s) 88, 699, 928, 980, 1052, 1072, 1177, 1196
NlaIV GGNNCC 8 cut(s) 229, 247, 471, 900, 973, 1106, 1187, 1557
NsiI ATGCAT 2 cut(s) 457, 1471
NspI RCATGY 1 cut(s) 1177
PaeR7I CTCGAG 1 cut(s) 891
PagI TCATGA 1 cut(s) 1192
PctI GAATGC 2 cut(s) 321, 1469
PfeI GAWTC 4 cut(s) 394, 1111, 1493, 1640
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 3 cut(s) 14, 62, 320
PleI GAGTC 2 cut(s) 1343, 1661
PpsI GAGTC 2 cut(s) 1343, 1661
PspN4I GGNNCC 8 cut(s) 229, 247, 471, 900, 973, 1106, 1187, 1557
PspPI GGNCC 2 cut(s) 228, 899
PstI CTGCAG 1 cut(s) 420
PsuI RGATCY 3 cut(s) 133, 148, 1555
PvuII CAGCTG 1 cut(s) 1716
RsaI GTAC 5 cut(s) 612, 1172, 1441, 1505, 1720
RsaNI GTAC 5 cut(s) 611, 1171, 1440, 1504, 1719
RseI CAYNNNNRTG 1 cut(s) 206
SatI GCNGC 3 cut(s) 13, 61, 319
Sau3AI GATC 8 cut(s) 133, 148, 800, 1098, 1300, 1399, 1555, 1729
Sau96I GGNCC 2 cut(s) 228, 899
SchI GAGTC 2 cut(s) 1343, 1661
SduI GDGCHC 4 cut(s) 415, 550, 976, 1802
SfaNI GCATC 1 cut(s) 1324
SfcI CTRYAG 3 cut(s) 416, 500, 933
Sfr274I CTCGAG 1 cut(s) 891
SinI GGWCC 2 cut(s) 228, 899
SlaI CTCGAG 1 cut(s) 891
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 891, 1526
SmoI CTYRAG 2 cut(s) 891, 1526
SpeI ACTAGT 1 cut(s) 811
SsiI CCGC 2 cut(s) 318, 1243
SspMI CTAG 7 cut(s) 119, 186, 509, 797, 812, 1539, 1810
StyI CCWWGG 3 cut(s) 796, 976, 1090
TaaI ACNGT 4 cut(s) 164, 985, 1420, 1508
TaqI TCGA 4 cut(s) 806, 892, 991, 1318
TatI WGTACW 2 cut(s) 1503, 1718
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 4 cut(s) 394, 1111, 1493, 1640
TscAI CASTG 3 cut(s) 420, 1263, 1492
TseI GCWGC 2 cut(s) 12, 60
TspDTI ATGAA 9 cut(s) 101, 128, 555, 576, 641, 1020, 1065, 1181, 1349
TspGWI ACGGA 1 cut(s) 1591
TspRI CASTG 3 cut(s) 420, 1263, 1492
Van91I CCANNNNNTGG 1 cut(s) 698
VpaK11BI GGWCC 2 cut(s) 228, 899
XapI RAATTY 6 cut(s) 33, 341, 376, 421, 1783, 1805
XceI RCATGY 1 cut(s) 1177
XhoI CTCGAG 1 cut(s) 891
XmaJI CCTAGG 1 cut(s) 796
XmiI GTMKAC 1 cut(s) 1406
XspI CTAG 7 cut(s) 119, 186, 509, 797, 812, 1539, 1810
Zsp2I ATGCAT 2 cut(s) 457, 1471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.