RLG00000020481
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011195
Physical Location & Seq
Reverse (-)
65482066 .. 65485189
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020481

Sequence Viewer

Length: 3036 bp
ATGCAGCTTTCTCTTTGCCATTTCCTTTTCTTGTTCTTGTGCTTCTCTGTTTCCATAACCAGTATAACTAGTTTGAACTCTGATGGGCTTGCTCTACTGTCCATATTAAACCACTGGACTTTTGTCCCCAAACCCATTTCCTCAAGCTGGAATCTTTCTGATTCCACACCATGCAAATGGGTAGGAATAGAATGCGACCCTGGCCACAATGTTTTTGCCTTGAATGTCTCTAGTTATAAAATTTCTGGTCAGTTAGGACCTGAAATTGGGAACTTGAGTCACTTAAAGACTCTTGTTTTGAGTTTCAATAATTTTTCTGGCCAAATTCCCATGGAGTTGGGCAATTGTAGTCTTCTTGAGACCTTGGATTTGAGTTATAACCAGTTGAGTGGAGTATTGCCCGAGAGTCTGAATAACCTTAAGAACCTGTCCTATCTACAAGTCAGTAAAAACAGTCTTGAGGGTACCATTTCTTTTGGTTCAGAAAATTGCAAGAATTTGACTTTCTTGGATTTGTCAACCAATCAGTTTAGTGGAGGTCTTCCATCAGCCCTTGCCAATTGTACTGGCTTAAGGGAATTTTCGGCTGCAGTTAACAATTTAGTTGGTCCAATACCGTCTTCCTTTGGCCTACTAGATAAGCTACAACTTCTATACCTTCCTGAGAATCAGCTGTCTGGAAAAATACCTCCAGAACTTGGCAAGTGTAAGTCCTTGACCGGACTACAATTGTATACAAACCAACTAGTTGGAGAAATTCCCGATGAGTTGGGAATGTTAACCAACTTGCAGGACCTTCAATTATTTCAAAACAACTTAACTGGTGAAATTCCAGTTAGCATATGGAAGATTCAAACTCTTCAATATGTCCATCTATACAAGAATAACCTCACTGGGGATCTGCCTTTAGAGATGACTGAGCTCAAGCAACTAGAAAACATCACATTGTACAACAACCAGTTTTATGGATTTGTACCTCAAGCTTTGGGGATTAACAGCAGTTTAGTTCAGTTAGATTTTATGTATAATATGTTCATTGGTAGCATCCCTCCAAATCTTTGCCGTGGAAAGAAGCTAAGGGTATTGAATATGGGGTTCAATCAATTTCAAGGTACCATACCTTCTGATGTCGGAAGTTGTTCAACTCTTTGGAGGTTGAGACTCGGACAGAATAACTTGACCGGAGTACTTCCGCAATTTGCAAAAAATTCTGACCTTTCATATATGGACATCAGCAGCAATGAGATTAGTGGAGAAATTCCATCAAGCTTGGGAAACTGTAGCAGCCTCACCTCCATCAAATTGGCCATGAATAAGTTAACAGGAGTTATACCAGAGGAGCTAGGGTACCTTGCAGATCTCCAAACTTTGGATCTTTCCCACAACAGTTTGGTGGGTGCTTTGCCTTCTAGCCTATCCAATTGTACCAAAATGCTGGAGTTTGATGTGGGGTCCAACTTCTTGAATGGCTCCATTCCTTTGAGTTTGAGTAGTTGGACAGGTTTATCTAAATTGGTATTAAGTGAGAACAGGTTTACTGGCGGCATTCCACCCTTCTTGTCAGAATTTGAAAAGCTTTCGGAGCTACAACTTGGTGGAAATTTGTTGGGAGGTGTGATTCCATCGTCAGTTGGAGCATTGCAGAATTTGTTTTATGCTCTGAACCTCAGCAATAATGCATTGACAGGTGTGGTTCCTTCAGAGCTTGGAAAGTTGACAATGCTACAGCGACTAGATTTATCTCATAACAATCTGACGGGGACTTTGAGAGCTCTTGATGGGATGAATTCATTGATTGAGGTTAATGTTTCATACAATAGCTTCACAGGTGCAGTACCAGAAACATTGATGAACCTGTTGAACTCATCCTCATTTTCCTTTTCCAGCAATCCCTCCCTATGTGTCAGTTACCTTCCATCATGTGGCTTCACTTGTGCTGGAAACAACACTATTAAGCTCTGTAATAGTCAATCAAGCAAGCAGAAAGGACTTAGTAAAGTGGAAATTGCATTTATAGCCCTAGGATCATCGATACTAGTTGTTTCTCTGCTGTATCTACTGATGTATTTGTTTTGCTTGCACAAAAGGACTAAGCAGGAGGTTGGGGTCTCAGCTCGAGAGGGTCCATCCTCCTTACTCTCCAAAGTCATGGAGGCTACAGAGAACCTTAGTGATAGATATATCATTGGGAGAGGAGCCCATGGAACTGTTTATAGAGCTTCTTTGGATGAAGACAAAGATTTTGCTGTCAAGAAGCTTGTATTTGAAGGGCATGAAGGAAGGCGTTTGAGCATGATTAGAGAACTTCAAACCCTTGGGAAGATCAGGCACCGGAATCTGGTCAAATTGGAAGACTTCTGGTTAAGAAAGGACTATGGCTTAATCTTGTATAGGTACATGCAAAATGGGAGCCTTCATGATGTTTTGCACGAAATTAGTCCCCCACTAATTCTGGAGTGGAGTGTCCGCTACAAGATAGCACTGGGAACTGCATACGGCTTAGAATATCTCCATTATGATTGTGATCCCCCAATAGTGCATCGAGATATCAAGCCAATGAACATTCTGTTAGACTCTGATATGGAAGCTCATATTGCTGATTTTGGAATTGCTAAGCTCTTGGATCAGTCTACTCCTTCAACGGTGTCCATCTCAGTTGTTGGTACAACCGGATATATCGCACCAGAAAATGCATTCAGAACGGCAAACAGTGTGGAATCTGATGTGTACAGTTATGGGGTTGTTTTACTTGAGCTGATAACTAGAAAGAAGGCATTGGATCCATCATTTATGGAGCAAACTGACATTGTAGGATGGGTTAGATCAGTGTGGGGCGACACAGAAAAAATTGATAGGATTGTTGATTCATGCCTTGTGGAGGAACTTCTGGACTCAGATATCAGGGAACAAATTGTTGATGTGCTTCTTGTGGCTTTCAGCTGTACTCATAATGATCCTAAGAAGCGACCCACGATGAGAGATGTTATCAAGCAATTATTAGATGTAAATTCCCCAATGAGGAGCACAAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1012

Amino Acids

111.33

Weight (kDa)

5.52

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 66 8.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 197 2.9e-09 Leucine-rich repeat region
LRR_8 PF13855 93 - 153 1.3e-06 Leucine rich repeat
LRR_8 PF13855 119 - 177 5.2e-06 Leucine rich repeat
LRR_14 PF23598 198 - 320 1e-06 Leucine-rich repeat region
LRR_14 PF23598 247 - 436 7.1e-08 Leucine-rich repeat region
LRR_8 PF13855 406 - 464 6.5e-08 Leucine rich repeat
LRR_8 PF13855 525 - 585 3e-08 Leucine rich repeat
Pkinase PF00069 728 - 998 2.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 728 - 999 5.3e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 237, 378
Acc65I GGTACC 3 cut(s) 464, 1112, 1347
AccB1I GGYRCC 4 cut(s) 464, 1112, 1347, 2328
AccB7I CCANNNNNTGG 3 cut(s) 698, 1369, 1922
AccI GTMKAC 2 cut(s) 734, 2630
AciI CCGC 3 cut(s) 1193, 1542, 2467
AclWI GGATC 8 cut(s) 906, 1380, 2032, 2519, 2631, 2774, 2787, 2948
AcoI YGGCCR 3 cut(s) 202, 319, 1305
AcuI CTGAAG 1 cut(s) 1683
AfiI CCNNNNNNNGG 9 cut(s) 147, 266, 698, 895, 1369, 1922, 2338, 2878, 3018
AflII CTTAAG 2 cut(s) 419, 571
AhlI ACTAGT 3 cut(s) 68, 745, 2035
AjnI CCWGG 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 604, 636
Alw21I GWGCWC 3 cut(s) 924, 1774, 3026
Alw26I GTCTC 4 cut(s) 232, 353, 1153, 2113
AlwI GGATC 8 cut(s) 906, 1380, 2032, 2519, 2631, 2774, 2787, 2948
Ama87I CYCGRG 2 cut(s) 401, 2115
AoxI GGCC 4 cut(s) 202, 319, 628, 1305
ApeKI GCWGC 4 cut(s) 4, 587, 1236, 1284
ArsI GACNNNNNNTTYG 8 cut(s) 280, 312, 352, 384, 1747, 1779, 2225, 2257
Asp700I GAANNNNTTC 1 cut(s) 1138
Asp718I GGTACC 3 cut(s) 464, 1112, 1347
AspA2I CCTAGG 1 cut(s) 2020
AspS9I GGNCC 5 cut(s) 257, 608, 793, 1452, 2123
AsuHPI GGTGA 2 cut(s) 836, 1282
AvaI CYCGRG 2 cut(s) 401, 2115
AvaII GGWCC 5 cut(s) 257, 608, 793, 1452, 2123
AvrII CCTAGG 1 cut(s) 2020
BalI TGGCCA 3 cut(s) 204, 321, 1307
BamHI GGATCC 1 cut(s) 2779
BanI GGYRCC 4 cut(s) 464, 1112, 1347, 2328
BanII GRGCYC 3 cut(s) 924, 1774, 2200
BarI GAAGNNNNNNTAC 2 cut(s) 1105, 1137
BbsI GAAGAC 5 cut(s) 344, 533, 612, 2238, 2358
Bbv12I GWGCWC 3 cut(s) 924, 1774, 3026
BbvCI CCTCAGC 1 cut(s) 1667
BbvI GCAGC 4 cut(s) 16, 574, 1248, 1296
BceAI ACGGC 3 cut(s) 1047, 2512, 2718
BciT130I CCWGG 1 cut(s) 201
BcoDI GTCTC 4 cut(s) 232, 353, 1153, 2113
BcuI ACTAGT 3 cut(s) 68, 745, 2035
BfmI CTRYAG 4 cut(s) 588, 1279, 1724, 2157
BfrI CTTAAG 2 cut(s) 419, 571
BglII AGATCT 1 cut(s) 1357
BisI GCNGC 5 cut(s) 5, 588, 1237, 1285, 1543
BlnI CCTAGG 1 cut(s) 2020
BlpI GCTNAGC 1 cut(s) 2613
BlsI GCNGC 5 cut(s) 6, 589, 1238, 1286, 1544
BmcAI AGTACT 1 cut(s) 1188
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 5 cut(s) 257, 608, 793, 1452, 2123
BmeT110I CYCGRG 2 cut(s) 401, 2115
BmgT120I GGNCC 5 cut(s) 257, 608, 793, 1452, 2123
BmrFI CCNGG 1 cut(s) 201
BmrI ACTGGG 2 cut(s) 903, 2492
BmsI GCATC 2 cut(s) 1053, 2548
BmuI ACTGGG 2 cut(s) 903, 2492
BoxI GACNNNNGTC 1 cut(s) 122
BpiI GAAGAC 5 cut(s) 344, 533, 612, 2238, 2358
BpmI CTGGAG 3 cut(s) 675, 1457, 2474
Bpu10I CCTNAGC 2 cut(s) 1076, 1667
Bpu1102I GCTNAGC 1 cut(s) 2613
BpuEI CTTGAG 7 cut(s) 127, 295, 377, 479, 908, 963, 2771
Bsa29I ATCGAT 1 cut(s) 2030
BsaBI GATNNNNATC 1 cut(s) 2523
BsaI GGTCTC 2 cut(s) 353, 2113
BsaJI CCNNGG 7 cut(s) 199, 330, 363, 1063, 2020, 2200, 2314
BsaWI WCCGGW 4 cut(s) 719, 1181, 2331, 2669
BsaXI ACNNNNNCTCC 2 cut(s) 1331, 1361
Bsc4I CCNNNNNNNGG 9 cut(s) 147, 266, 698, 895, 1369, 1922, 2338, 2878, 3018
Bse3DI GCAATG 2 cut(s) 1246, 1637
Bse8I GATNNNNATC 1 cut(s) 2523
BseBI CCWGG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 2030
BseDI CCNNGG 7 cut(s) 199, 330, 363, 1063, 2020, 2200, 2314
BseGI GGATG 6 cut(s) 1044, 1788, 1865, 2126, 2233, 2819
BseJI GATNNNNATC 1 cut(s) 2523
BseLI CCNNNNNNNGG 9 cut(s) 147, 266, 698, 895, 1369, 1922, 2338, 2878, 3018
BseMI GCAATG 2 cut(s) 1246, 1637
BseMII CTCAG 6 cut(s) 654, 909, 1681, 2124, 2667, 2907
BseRI GAGGAG 3 cut(s) 1352, 2208, 3034
BseXI GCAGC 4 cut(s) 16, 574, 1248, 1296
BsgI GTGCAG 1 cut(s) 1851
BshFI GGCC 4 cut(s) 204, 321, 630, 1307
BshNI GGYRCC 4 cut(s) 464, 1112, 1347, 2328
BshVI ATCGAT 1 cut(s) 2030
BsiHKAI GWGCWC 3 cut(s) 924, 1774, 3026
BsiHKCI CYCGRG 2 cut(s) 401, 2115
BsiSI CCGG 4 cut(s) 720, 1182, 2332, 2670
BslFI GGGAC 3 cut(s) 110, 1774, 2424
BslI CCNNNNNNNGG 9 cut(s) 147, 266, 698, 895, 1369, 1922, 2338, 2878, 3018
BsmAI GTCTC 4 cut(s) 232, 353, 1153, 2113
BsmFI GGGAC 3 cut(s) 110, 1774, 2424
BsmI GAATGC 3 cut(s) 197, 1545, 2693
BsnI GGCC 4 cut(s) 204, 321, 630, 1307
Bso31I GGTCTC 2 cut(s) 353, 2113
BsoBI CYCGRG 2 cut(s) 401, 2115
Bsp1286I GDGCHC 4 cut(s) 924, 1774, 2200, 3026
Bsp1407I TGTACA 2 cut(s) 948, 2727
Bsp1720I GCTNAGC 1 cut(s) 2613
Bsp19I CCATGG 2 cut(s) 330, 2200
BspACI CCGC 3 cut(s) 1193, 1542, 2467
BspANI GGCC 4 cut(s) 204, 321, 630, 1307
BspCNI CTCAG 6 cut(s) 655, 910, 1680, 2123, 2666, 2906
BspDI ATCGAT 1 cut(s) 2030
BspHI TCATGA 1 cut(s) 2416
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 8 cut(s) 906, 1380, 2032, 2519, 2631, 2774, 2787, 2948
BspT107I GGYRCC 4 cut(s) 464, 1112, 1347, 2328
BspTI CTTAAG 2 cut(s) 419, 571
BspTNI GGTCTC 2 cut(s) 353, 2113
BsrDI GCAATG 2 cut(s) 1246, 1637
BsrGI TGTACA 2 cut(s) 948, 2727
BssECI CCNNGG 7 cut(s) 199, 330, 363, 1063, 2020, 2200, 2314
BssNAI GTATAC 1 cut(s) 735
BssT1I CCWWGG 5 cut(s) 330, 363, 2020, 2200, 2314
Bst1107I GTATAC 1 cut(s) 735
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 9 cut(s) 99, 455, 618, 1280, 1388, 2209, 2644, 2711, 2732
Bst6I CTCTTC 1 cut(s) 864
BstAFI CTTAAG 2 cut(s) 419, 571
BstAUI TGTACA 2 cut(s) 948, 2727
BstC8I GCNNGC 3 cut(s) 90, 1979, 2078
BstDSI CCRYGG 3 cut(s) 330, 1063, 2200
BstENI CCTNNNNNAGG 1 cut(s) 2876
BstF5I GGATG 6 cut(s) 1044, 1788, 1865, 2126, 2233, 2819
BstMAI GTCTC 4 cut(s) 232, 353, 1153, 2113
BstMWI GCNNNNNNNGC 4 cut(s) 201, 1582, 2015, 2594
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 1 cut(s) 2401
BstPAI GACNNNNGTC 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 4 cut(s) 588, 1279, 1724, 2157
BstV1I GCAGC 4 cut(s) 16, 574, 1248, 1296
BstV2I GAAGAC 5 cut(s) 344, 533, 612, 2238, 2358
BstX2I RGATCY 4 cut(s) 898, 1357, 1372, 2779
BstXI CCANNNNNNTGG 8 cut(s) 177, 337, 389, 749, 965, 1303, 1435, 2149
BstYI RGATCY 4 cut(s) 898, 1357, 1372, 2779
BstZ17I GTATAC 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 2030
BsuRI GGCC 4 cut(s) 204, 321, 630, 1307
BsuTUI ATCGAT 1 cut(s) 2030
BtgI CCRYGG 3 cut(s) 330, 1063, 2200
BtsCI GGATG 6 cut(s) 1044, 1788, 1865, 2126, 2233, 2819
BtsIMutI CAGTG 5 cut(s) 112, 891, 2480, 2716, 2832
Cac8I GCNNGC 3 cut(s) 90, 1979, 2078
CciI TCATGA 1 cut(s) 2416
Cfr13I GGNCC 5 cut(s) 257, 608, 793, 1452, 2123
ClaI ATCGAT 1 cut(s) 2030
CspCI CAANNNNNGTGG 4 cut(s) 1539, 1574, 2694, 2729
EaeI YGGCCR 3 cut(s) 202, 319, 1305
Eam1104I CTCTTC 1 cut(s) 864
EarI CTCTTC 1 cut(s) 864
Ecl136II GAGCTC 2 cut(s) 922, 1772
Eco130I CCWWGG 5 cut(s) 330, 363, 2020, 2200, 2314
Eco24I GRGCYC 3 cut(s) 924, 1774, 2200
Eco31I GGTCTC 2 cut(s) 353, 2113
Eco32I GATATC 2 cut(s) 2548, 2899
Eco47I GGWCC 5 cut(s) 257, 608, 793, 1452, 2123
Eco53kI GAGCTC 2 cut(s) 922, 1772
Eco57I CTGAAG 1 cut(s) 1683
Eco88I CYCGRG 2 cut(s) 401, 2115
EcoICRI GAGCTC 2 cut(s) 922, 1772
EcoNI CCTNNNNNAGG 1 cut(s) 2876
EcoO109I RGGNCCY 2 cut(s) 257, 793
EcoRI GAATTC 1 cut(s) 1786
EcoRII CCWGG 1 cut(s) 199
EcoRV GATATC 2 cut(s) 2548, 2899
EcoT14I CCWWGG 5 cut(s) 330, 363, 2020, 2200, 2314
EcoT22I ATGCAT 2 cut(s) 1681, 2695
EcoT38I GRGCYC 3 cut(s) 924, 1774, 2200
ErhI CCWWGG 5 cut(s) 330, 363, 2020, 2200, 2314
FaqI GGGAC 3 cut(s) 110, 1774, 2424
FauNDI CATATG 1 cut(s) 842
FblI GTMKAC 2 cut(s) 734, 2630
Fnu4HI GCNGC 5 cut(s) 5, 588, 1237, 1285, 1543
FokI GGATG 6 cut(s) 1031, 1795, 1852, 2113, 2240, 2826
FriOI GRGCYC 3 cut(s) 924, 1774, 2200
Fsp4HI GCNGC 5 cut(s) 5, 588, 1237, 1285, 1543
GluI GCNGC 5 cut(s) 5, 588, 1237, 1285, 1543
GsuI CTGGAG 3 cut(s) 675, 1457, 2474
HaeIII GGCC 4 cut(s) 204, 321, 630, 1307
HapII CCGG 4 cut(s) 720, 1182, 2332, 2670
HincII GTYRAC 5 cut(s) 519, 595, 780, 1320, 1716
HindII GTYRAC 5 cut(s) 519, 595, 780, 1320, 1716
HindIII AAGCTT 4 cut(s) 981, 1267, 1574, 2255
HpaI GTTAAC 3 cut(s) 595, 780, 1320
HpaII CCGG 4 cut(s) 720, 1182, 2332, 2670
HphI GGTGA 2 cut(s) 836, 1282
Hpy166II GTNNAC 9 cut(s) 519, 595, 735, 780, 1320, 1536, 1716, 2631, 2728
Hpy8I GTNNAC 9 cut(s) 519, 595, 735, 780, 1320, 1536, 1716, 2631, 2728
HpyCH4III ACNGT 9 cut(s) 99, 455, 618, 1280, 1388, 2209, 2644, 2711, 2732
HpyF10VI GCNNNNNNNGC 4 cut(s) 201, 1582, 2015, 2594
KpnI GGTACC 3 cut(s) 468, 1116, 1351
KspAI GTTAAC 3 cut(s) 595, 780, 1320
LmnI GCTCC 8 cut(s) 1339, 1475, 1582, 1634, 2195, 2409, 2794, 3021
Lsp1109I GCAGC 4 cut(s) 16, 574, 1248, 1296
LweI GCATC 2 cut(s) 1053, 2548
MaeIII GTNAC 2 cut(s) 278, 1907
MboII GAAGA 8 cut(s) 344, 533, 612, 851, 859, 2243, 2332, 2363
MfeI CAATTG 4 cut(s) 343, 559, 728, 1420
MflI RGATCY 4 cut(s) 898, 1357, 1372, 2779
MhlI GDGCHC 4 cut(s) 924, 1774, 2200, 3026
MlsI TGGCCA 3 cut(s) 204, 321, 1307
MluNI TGGCCA 3 cut(s) 204, 321, 1307
MlyI GAGTC 6 cut(s) 283, 286, 415, 1155, 2567, 2885
MmeI TCCRAC 5 cut(s) 730, 1111, 1475, 1479, 1612
Mox20I TGGCCA 3 cut(s) 204, 321, 1307
Mph1103I ATGCAT 2 cut(s) 1681, 2695
MroXI GAANNNNTTC 1 cut(s) 1138
MscI TGGCCA 3 cut(s) 204, 321, 1307
MslI CAYNNNNRTG 1 cut(s) 175
Msp20I TGGCCA 3 cut(s) 204, 321, 1307
MspA1I CMGCKG 2 cut(s) 673, 2940
MspCI CTTAAG 2 cut(s) 419, 571
MspI CCGG 4 cut(s) 720, 1182, 2332, 2670
MspR9I CCNGG 1 cut(s) 201
MunI CAATTG 4 cut(s) 343, 559, 728, 1420
Mva1269I GAATGC 3 cut(s) 197, 1545, 2693
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 201, 1582, 2015, 2594
NcoI CCATGG 2 cut(s) 330, 2200
NdeI CATATG 1 cut(s) 842
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 2 cut(s) 1681, 2695
NspI RCATGY 1 cut(s) 2401
PaeR7I CTCGAG 1 cut(s) 2115
PagI TCATGA 1 cut(s) 2416
PctI GAATGC 3 cut(s) 197, 1545, 2693
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 8 cut(s) 151, 161, 667, 850, 1618, 2335, 2717, 2864
PflMI CCANNNNNTGG 3 cut(s) 698, 1369, 1922
PkrI GCNGC 5 cut(s) 6, 589, 1238, 1286, 1544
PleI GAGTC 6 cut(s) 283, 285, 414, 1155, 2567, 2885
PpsI GAGTC 6 cut(s) 283, 285, 414, 1155, 2567, 2885
PpuMI RGGWCCY 2 cut(s) 257, 793
PshAI GACNNNNGTC 1 cut(s) 122
PsiI TTATAA 2 cut(s) 237, 378
Psp124BI GAGCTC 2 cut(s) 924, 1774
Psp5II RGGWCCY 2 cut(s) 257, 793
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspPI GGNCC 5 cut(s) 257, 608, 793, 1452, 2123
PspPPI RGGWCCY 2 cut(s) 257, 793
PsrI GAACNNNNNNTAC 2 cut(s) 1016, 1048
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 4 cut(s) 898, 1357, 1372, 2779
PvuII CAGCTG 2 cut(s) 673, 2940
RseI CAYNNNNRTG 1 cut(s) 175
SacI GAGCTC 2 cut(s) 924, 1774
SatI GCNGC 5 cut(s) 5, 588, 1237, 1285, 1543
Sau96I GGNCC 5 cut(s) 257, 608, 793, 1452, 2123
ScaI AGTACT 1 cut(s) 1188
SchI GAGTC 6 cut(s) 283, 286, 415, 1155, 2567, 2885
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 4 cut(s) 924, 1774, 2200, 3026
SfaNI GCATC 2 cut(s) 1053, 2548
SfcI CTRYAG 4 cut(s) 588, 1279, 1724, 2157
Sfr274I CTCGAG 1 cut(s) 2115
SinI GGWCC 5 cut(s) 257, 608, 793, 1452, 2123
SlaI CTCGAG 1 cut(s) 2115
SmiMI CAYNNNNRTG 1 cut(s) 175
SpeI ACTAGT 3 cut(s) 68, 745, 2035
SsiI CCGC 3 cut(s) 1193, 1542, 2467
SstI GAGCTC 2 cut(s) 924, 1774
StyD4I CCNGG 1 cut(s) 199
StyI CCWWGG 5 cut(s) 330, 363, 2020, 2200, 2314
TaaI ACNGT 9 cut(s) 99, 455, 618, 1280, 1388, 2209, 2644, 2711, 2732
TaqI TCGA 3 cut(s) 2030, 2116, 2542
TatI WGTACW 5 cut(s) 563, 948, 1186, 2727, 2942
TauI GCSGC 1 cut(s) 1545
TfiI GAWTC 8 cut(s) 151, 161, 667, 850, 1618, 2335, 2717, 2864
TscAI CASTG 5 cut(s) 119, 898, 2487, 2716, 2832
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 4 cut(s) 4, 587, 1236, 1284
Tsp45I GTSAC 1 cut(s) 278
TspRI CASTG 5 cut(s) 119, 898, 2487, 2716, 2832
Van91I CCANNNNNTGG 3 cut(s) 698, 1369, 1922
Vha464I CTTAAG 2 cut(s) 419, 571
VpaK11BI GGWCC 5 cut(s) 257, 608, 793, 1452, 2123
XagI CCTNNNNNAGG 1 cut(s) 2876
XceI RCATGY 1 cut(s) 2401
XcmI CCANNNNNNNNNTGG 1 cut(s) 840
XhoI CTCGAG 1 cut(s) 2115
XmaJI CCTAGG 1 cut(s) 2020
XmiI GTMKAC 2 cut(s) 734, 2630
XmnI GAANNNNTTC 1 cut(s) 1138
ZrmI AGTACT 1 cut(s) 1188
Zsp2I ATGCAT 2 cut(s) 1681, 2695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.