MD09G1158300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011195
Physical Location & Seq
Forward (+)
12649687 .. 12652097
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1158300.v1.1.491

Sequence Viewer

Length: 2127 bp
ATGACTGAGCTGAAGCAGCTGCAGAATATTTCGTTGTTTAACAACCTGTTTTCTGGAGTTATACCTCAAGGTCTGGGGATTAACAGCAGTCTAGTGCAGTTAGATTTTCTGAATAATAACTTCACTGGTACAATCCCTCCAAATCTTTGCCATGGAAAGCGATTAAGGGTGTTGACTACGGCTTCCAATCGACTTCAAGGCTCCATACCTTCTGATGTTGGAAATTGCTCCACTCTTTGGAGGTTGAAGCTTGAACAGAATAACCTCAGTGGAGCTCTCCCGGAATTTGCAGAAAATCCAAACTTCGATTACATGGACATCAGCAGCAATGAGATTAGTGGAGCTATTCCATCAAGCTTGGGAAACTGTGGCAACCTCACAACTATCAATTTGTCCGCAAACAAGTTAACTGGAGCTATACCGCCGGAGCTGGGGAATCTTGCAGAGCTCCGTACATTGGTTCTTTTCCAGAACAATTTGGTTGGTTCTCTGCCTCCTCAACTATCTAAGTGTACCAAAATGGATAAGTTTGATGTGCGGTCCAACTTGTTGAATGGCTCCATTCCGTCAATTCTGAGAAGTTGGACAGGTTTATCAACACTGATTTTAAGCGACAACAGCTTCACTGGTGGCATCCCAACTTTCTTGTCAGACTTCGAAAAGCTTTCAGAGCTACAACTTGGTGGAAATCTTTTCGGAGGTGTGATTCCACCATCAATTGGAGCATTGCAGAGTATCTTTTATGCTTTAAATCTTAGCAACAATGGATTGACAGGTCCGCTTCCTCCGGAGCTAGGGAAGCTGATAAGGCTACAACAATTAGATCTTTCTCATAACAATTTGACAGGGACTTTAAAAGTGCTCGACGGTATGAGTTCACTAACTGAGGTTAATGTTTCAGACAACAACTTCACAGGCCCCGTACCAGAGACGTTGATGAAGCTGTTGAACTCATCCCCATCGTCATTTTTGGGCAATCCCTTCATATGTGTCAATAACCTTCCATCATGTGGCTCAACGTGCGCAGGAAACAGCAGTTTTAAGCCTTGCAACAGCCCACCAAGCAACCGGAAAGGCCTTAGTAAAGTGCAACTTGCATTTATAGCTCTAGGATCCTCGATATTTGTTGTTTTTGTGCTTTATGGGCTGATTTATCTGTTCCTCTGGCGCAAAAAGGCCAAGCACGATCTTGAGATCTCAGCTCAAGAGGGGCCATCTGCCTTGCTGAACAAGGTACTGGAGGCTACAGAAAACCTGAATGGTCACTATATCATTGGGAGAGGAGCCCATGGAACAGTCTATAAGGCCTCCTTGGCGACAGACAAAGATTATGCAGTCAAGAAGCTTCAATTTGCAGGGCACGAAGGAAGGCGTTTGAGCATGGTTCGAGAAATTCAGACCCTTGGGTCAATTAGGCACCGGAATCTGGTTAGATTGGAAGACTTCTGGTTAAGAAAAGACCACGGTTTAATCTTGTATAGGTACATGCAAAATGGGAGCCTTCATGATGTTTTACATGAAATTGAACCCCCACCAACTCTTGAGTGGAGTGTTCGCTACAGGATAGCGCTTGGAACTGCATACGGGTTGGAATATCTCCATTATGATTGTGATCCCCCGATAGTGCATCGAGACATCAAACCTATGAACATCCTCTTGGACTCTGATATGGAGCCCCATATTGCTGATTTTGGCATTGCTAAACTTCTGGATCACCAATCTTCTGCACCAACGACATCCATTGCGGTTGTGGGTACAACCGGATATATTGCACCAGAAAATGCATTTAGAACGGCAAAGGGCGTGGAATCTGATGTGTACAGTTACGGGATTGTTTTACTTGAGCTGATAACTAGAAAGAAGGCATTGGATCCATCATTTATGGAGCAAACTGACATTGCAGGATGGGCCAAGTCAGTGTGGAGCAGCACGGAACAAATTGATCAGATCGTCGATTCAAGCCTTAAGGAGGAACTTCTGGATTCAACGACCATGGATCAAGTTATCGAGGTGCTTATGGTGGCTTTCAGATGTACTGAGAAAGATCCGAAAAGGAGGCCCACGATGAGAGCTGTTATCAAGCAATTGTTAGATGCAGATTCCCAAGTGAGAAGCACAAAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

709

Amino Acids

77.63

Weight (kDa)

6.42

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 103 - 160 6.3e-07 Leucine rich repeat
Pkinase PF00069 424 - 694 8.3e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 696 1.7e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1405
Acc16I TGCGCA 1 cut(s) 1024
AccB1I GGYRCC 1 cut(s) 1416
AccB7I CCANNNNNTGG 3 cut(s) 237, 719, 1010
AccIII TCCGGA 1 cut(s) 787
AciI CCGC 5 cut(s) 396, 422, 538, 779, 1745
AclWI GGATC 8 cut(s) 1107, 1120, 1607, 1719, 1865, 1878, 2004, 2039
AcsI RAATTY 2 cut(s) 284, 1392
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 9 cut(s) 130, 454, 514, 924, 1236, 1484, 1756, 1820, 2035
AfeI AGCGCT 1 cut(s) 1569
AfiI CCNNNNNNNGG 8 cut(s) 237, 431, 457, 719, 794, 1010, 1426, 1969
AflII CTTAAG 1 cut(s) 1964
AgsI TTSAA 9 cut(s) 197, 247, 254, 553, 949, 1349, 1526, 1959, 1986
AjuI GAANNNNNNNTTGG 2 cut(s) 1640, 1672
Alw21I GWGCWC 3 cut(s) 277, 450, 864
Alw26I GTCTC 2 cut(s) 923, 1626
AlwI GGATC 8 cut(s) 1107, 1120, 1607, 1719, 1865, 1878, 2004, 2039
Aor13HI TCCGGA 1 cut(s) 787
Aor51HI AGCGCT 1 cut(s) 1569
AoxI GGCC 7 cut(s) 916, 1075, 1176, 1211, 1305, 1908, 2057
ApeKI GCWGC 4 cut(s) 16, 19, 324, 1926
ApoI RAATTY 2 cut(s) 284, 1392
AspLEI GCGC 3 cut(s) 1025, 1170, 1570
AspS9I GGNCC 6 cut(s) 540, 776, 917, 1211, 1908, 2058
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 1706
AsuII TTCGAA 1 cut(s) 657
AvaII GGWCC 2 cut(s) 540, 776
BaeGI GKGCMC 1 cut(s) 1362
BamHI GGATCC 2 cut(s) 1112, 1870
BanI GGYRCC 1 cut(s) 1416
BanII GRGCYC 4 cut(s) 277, 450, 1288, 1677
BbsI GAAGAC 1 cut(s) 1446
Bbv12I GWGCWC 3 cut(s) 277, 450, 864
BbvI GCAGC 4 cut(s) 6, 28, 336, 1938
BccI CCATC 7 cut(s) 358, 721, 967, 1012, 1222, 1882, 1899
BceAI ACGGC 2 cut(s) 195, 1809
BclI TGATCA 1 cut(s) 1942
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 923, 1626
BfaI CTAG 5 cut(s) 92, 794, 1109, 1854, 2125
BfmI CTRYAG 3 cut(s) 20, 1245, 1558
BfoI RGCGCY 1 cut(s) 1571
BfrI CTTAAG 1 cut(s) 1964
BglI GCCNNNNNGGC 1 cut(s) 1313
BglII AGATCT 2 cut(s) 823, 1194
BisI GCNGC 4 cut(s) 17, 20, 325, 1927
BlsI GCNGC 4 cut(s) 18, 21, 326, 1928
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 2 cut(s) 540, 776
BmgT120I GGNCC 6 cut(s) 540, 776, 917, 1211, 1908, 2058
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 3 cut(s) 642, 1636, 2083
BpiI GAAGAC 1 cut(s) 1446
BpmI CTGGAG 3 cut(s) 75, 432, 1259
Bpu14I TTCGAA 1 cut(s) 657
BpuEI CTTGAG 5 cut(s) 51, 1188, 1211, 1562, 1862
BpuMI CCSGG 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 1611
BsaJI CCNNGG 6 cut(s) 151, 1288, 1311, 1402, 1462, 1992
BsaWI WCCGGW 4 cut(s) 787, 1068, 1419, 1760
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 8 cut(s) 237, 431, 457, 719, 794, 1010, 1426, 1969
Bse1I ACTGG 4 cut(s) 130, 415, 631, 1242
Bse3DI GCAATG 5 cut(s) 334, 725, 1695, 1740, 1896
Bse8I GATNNNNATC 1 cut(s) 1611
BseAI TCCGGA 1 cut(s) 787
BseDI CCNNGG 6 cut(s) 151, 1288, 1311, 1402, 1462, 1992
BseGI GGATG 5 cut(s) 633, 953, 1650, 1736, 1910
BseJI GATNNNNATC 1 cut(s) 1611
BseLI CCNNNNNNNGG 8 cut(s) 237, 431, 457, 719, 794, 1010, 1426, 1969
BseMI GCAATG 5 cut(s) 334, 725, 1695, 1740, 1896
BseMII CTCAG 5 cut(s) 280, 566, 876, 1212, 2028
BseNI ACTGG 4 cut(s) 130, 415, 631, 1242
BseRI GAGGAG 2 cut(s) 486, 1296
BseSI GKGCMC 1 cut(s) 1362
BseXI GCAGC 4 cut(s) 6, 28, 336, 1938
BseYI CCCAGC 1 cut(s) 430
BsgI GTGCAG 2 cut(s) 116, 1710
BshFI GGCC 7 cut(s) 918, 1077, 1178, 1213, 1307, 1910, 2059
BshNI GGYRCC 1 cut(s) 1416
BsiHKAI GWGCWC 3 cut(s) 277, 450, 864
BsiSI CCGG 6 cut(s) 281, 425, 788, 1069, 1420, 1761
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 8 cut(s) 237, 431, 457, 719, 794, 1010, 1426, 1969
BsmAI GTCTC 2 cut(s) 923, 1626
BsmBI CGTCTC 1 cut(s) 923
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 7 cut(s) 918, 1077, 1178, 1213, 1307, 1910, 2059
Bsp119I TTCGAA 1 cut(s) 657
Bsp1286I GDGCHC 6 cut(s) 277, 450, 864, 1288, 1362, 1677
Bsp13I TCCGGA 1 cut(s) 787
Bsp1407I TGTACA 1 cut(s) 1818
Bsp19I CCATGG 3 cut(s) 151, 1288, 1992
BspACI CCGC 5 cut(s) 396, 422, 538, 779, 1745
BspANI GGCC 7 cut(s) 918, 1077, 1178, 1213, 1307, 1910, 2059
BspCNI CTCAG 5 cut(s) 279, 567, 877, 1211, 2029
BspEI TCCGGA 1 cut(s) 787
BspHI TCATGA 1 cut(s) 1504
BspMAI CTGCAG 1 cut(s) 24
BspPI GGATC 8 cut(s) 1107, 1120, 1607, 1719, 1865, 1878, 2004, 2039
BspT104I TTCGAA 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 1416
BspTI CTTAAG 1 cut(s) 1964
BsrDI GCAATG 5 cut(s) 334, 725, 1695, 1740, 1896
BsrGI TGTACA 1 cut(s) 1818
BsrI ACTGG 4 cut(s) 130, 415, 631, 1242
BssECI CCNNGG 6 cut(s) 151, 1288, 1311, 1402, 1462, 1992
BssT1I CCWWGG 5 cut(s) 151, 1288, 1311, 1402, 1992
Bst4CI ACNGT 5 cut(s) 368, 869, 1297, 1466, 1823
BstAFI CTTAAG 1 cut(s) 1964
BstAUI TGTACA 1 cut(s) 1818
BstBI TTCGAA 1 cut(s) 657
BstDEI CTNAG 9 cut(s) 6, 266, 507, 575, 755, 885, 1079, 1198, 2037
BstDSI CCRYGG 4 cut(s) 151, 1288, 1462, 1992
BstENI CCTNNNNNAGG 1 cut(s) 1967
BstF5I GGATG 5 cut(s) 633, 953, 1650, 1736, 1910
BstH2I RGCGCY 1 cut(s) 1571
BstHHI GCGC 3 cut(s) 1025, 1170, 1570
BstMAI GTCTC 2 cut(s) 923, 1626
BstNSI RCATGY 1 cut(s) 1489
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 3 cut(s) 20, 1245, 1558
BstSLI GKGCMC 1 cut(s) 1362
BstV1I GCAGC 4 cut(s) 6, 28, 336, 1938
BstV2I GAAGAC 1 cut(s) 1446
BstX2I RGATCY 5 cut(s) 823, 1112, 1194, 1870, 2044
BstYI RGATCY 5 cut(s) 823, 1112, 1194, 1870, 2044
BsuRI GGCC 7 cut(s) 918, 1077, 1178, 1213, 1307, 1910, 2059
BtgI CCRYGG 4 cut(s) 151, 1288, 1462, 1992
BtsCI GGATG 5 cut(s) 633, 953, 1650, 1736, 1910
BtsIMutI CAGTG 5 cut(s) 123, 274, 599, 624, 1923
CciI TCATGA 1 cut(s) 1504
CfoI GCGC 3 cut(s) 1025, 1170, 1570
Cfr13I GGNCC 6 cut(s) 540, 776, 917, 1211, 1908, 2058
Csp6I GTAC 9 cut(s) 129, 453, 513, 923, 1235, 1483, 1755, 1819, 2034
CspCI CAANNNNNGTGG 2 cut(s) 1785, 1820
CviAII CATG 9 cut(s) 152, 313, 1008, 1289, 1381, 1486, 1505, 1517, 1993
CviQI GTAC 9 cut(s) 129, 453, 513, 923, 1235, 1483, 1755, 1819, 2034
DdeI CTNAG 9 cut(s) 6, 266, 507, 575, 755, 885, 1079, 1198, 2037
DraI TTTAAA 2 cut(s) 750, 855
DrdI GACNNNNNNGTC 1 cut(s) 1405
DseDI GACNNNNNNGTC 1 cut(s) 1405
Ecl136II GAGCTC 2 cut(s) 275, 448
Eco130I CCWWGG 5 cut(s) 151, 1288, 1311, 1402, 1992
Eco147I AGGCCT 2 cut(s) 1077, 1307
Eco24I GRGCYC 4 cut(s) 277, 450, 1288, 1677
Eco47I GGWCC 2 cut(s) 540, 776
Eco47III AGCGCT 1 cut(s) 1569
Eco53kI GAGCTC 2 cut(s) 275, 448
Eco57I CTGAAG 1 cut(s) 32
EcoICRI GAGCTC 2 cut(s) 275, 448
EcoNI CCTNNNNNAGG 1 cut(s) 1967
EcoO109I RGGNCCY 1 cut(s) 917
EcoT14I CCWWGG 5 cut(s) 151, 1288, 1311, 1402, 1992
EcoT22I ATGCAT 1 cut(s) 1786
EcoT38I GRGCYC 4 cut(s) 277, 450, 1288, 1677
ErhI CCWWGG 5 cut(s) 151, 1288, 1311, 1402, 1992
Esp3I CGTCTC 1 cut(s) 923
FaeI CATG 9 cut(s) 155, 316, 1011, 1292, 1384, 1489, 1508, 1520, 1996
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1295, 1327
FaqI GGGAC 1 cut(s) 862
FatI CATG 9 cut(s) 151, 312, 1007, 1288, 1380, 1485, 1504, 1516, 1992
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 1 cut(s) 1942
Fnu4HI GCNGC 4 cut(s) 17, 20, 325, 1927
FokI GGATG 5 cut(s) 620, 940, 1637, 1723, 1917
FriOI GRGCYC 4 cut(s) 277, 450, 1288, 1677
Fsp4HI GCNGC 4 cut(s) 17, 20, 325, 1927
FspBI CTAG 5 cut(s) 92, 794, 1109, 1854, 2125
FspI TGCGCA 1 cut(s) 1024
GlaI GCGC 3 cut(s) 1024, 1169, 1569
GluI GCNGC 4 cut(s) 17, 20, 325, 1927
GsaI CCCAGC 1 cut(s) 434
GsuI CTGGAG 3 cut(s) 75, 432, 1259
HaeII RGCGCY 1 cut(s) 1571
HaeIII GGCC 7 cut(s) 918, 1077, 1178, 1213, 1307, 1910, 2059
HapII CCGG 6 cut(s) 281, 425, 788, 1069, 1420, 1761
HhaI GCGC 3 cut(s) 1025, 1170, 1570
Hin1II CATG 9 cut(s) 155, 316, 1011, 1292, 1384, 1489, 1508, 1520, 1996
Hin6I GCGC 3 cut(s) 1023, 1168, 1568
HinP1I GCGC 3 cut(s) 1023, 1168, 1568
HincII GTYRAC 2 cut(s) 174, 408
HindII GTYRAC 2 cut(s) 174, 408
HindIII AAGCTT 4 cut(s) 248, 355, 662, 1343
HinfI GANTC 8 cut(s) 436, 706, 1423, 1661, 1808, 1955, 1982, 2099
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 6 cut(s) 281, 425, 788, 1069, 1420, 1761
HphI GGTGA 1 cut(s) 1706
Hpy166II GTNNAC 5 cut(s) 174, 408, 513, 878, 1819
Hpy8I GTNNAC 5 cut(s) 174, 408, 513, 878, 1819
Hpy99I CGWCG 2 cut(s) 869, 1955
HpyAV CCTTC 7 cut(s) 219, 991, 1010, 1358, 1362, 1511, 1855
HpyCH4III ACNGT 5 cut(s) 368, 869, 1297, 1466, 1823
HpyCH4IV ACGT 2 cut(s) 932, 1019
HpyF3I CTNAG 9 cut(s) 6, 266, 507, 575, 755, 885, 1079, 1198, 2037
HpySE526I ACGT 2 cut(s) 932, 1019
Hsp92II CATG 9 cut(s) 155, 316, 1011, 1292, 1384, 1489, 1508, 1520, 1996
HspAI GCGC 3 cut(s) 1023, 1168, 1568
Kpn2I TCCGGA 1 cut(s) 787
Ksp22I TGATCA 1 cut(s) 1942
KspAI GTTAAC 1 cut(s) 408
Lsp1109I GCAGC 4 cut(s) 6, 28, 336, 1938
LweI GCATC 3 cut(s) 642, 1636, 2083
MaeI CTAG 5 cut(s) 92, 794, 1109, 1854, 2125
MaeII ACGT 2 cut(s) 932, 1019
MaeIII GTNAC 2 cut(s) 1262, 1823
MboII GAAGA 2 cut(s) 1451, 1713
MfeI CAATTG 2 cut(s) 717, 2084
MflI RGATCY 5 cut(s) 823, 1112, 1194, 1870, 2044
MhlI GDGCHC 6 cut(s) 277, 450, 864, 1288, 1362, 1677
MlyI GAGTC 1 cut(s) 1655
MmeI TCCRAC 4 cut(s) 199, 563, 567, 1569
Mph1103I ATGCAT 1 cut(s) 1786
MroI TCCGGA 1 cut(s) 787
MspA1I CMGCKG 1 cut(s) 19
MspCI CTTAAG 1 cut(s) 1964
MspI CCGG 6 cut(s) 281, 425, 788, 1069, 1420, 1761
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 2 cut(s) 717, 2084
NciI CCSGG 1 cut(s) 281
NcoI CCATGG 3 cut(s) 151, 1288, 1992
NdeI CATATG 1 cut(s) 986
NlaIII CATG 9 cut(s) 155, 316, 1011, 1292, 1384, 1489, 1508, 1520, 1996
NmuCI GTSAC 1 cut(s) 1262
NsbI TGCGCA 1 cut(s) 1024
NsiI ATGCAT 1 cut(s) 1786
NspI RCATGY 1 cut(s) 1489
NspV TTCGAA 1 cut(s) 657
PagI TCATGA 1 cut(s) 1504
PceI AGGCCT 2 cut(s) 1077, 1307
PfeI GAWTC 7 cut(s) 436, 706, 1423, 1808, 1955, 1982, 2099
PflMI CCANNNNNTGG 3 cut(s) 237, 719, 1010
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 4 cut(s) 18, 21, 326, 1928
PleI GAGTC 1 cut(s) 1655
PpsI GAGTC 1 cut(s) 1655
Psp124BI GAGCTC 2 cut(s) 277, 450
PspFI CCCAGC 1 cut(s) 430
PspPI GGNCC 6 cut(s) 540, 776, 917, 1211, 1908, 2058
PstI CTGCAG 1 cut(s) 24
PsuI RGATCY 5 cut(s) 823, 1112, 1194, 1870, 2044
PvuII CAGCTG 1 cut(s) 19
RsaI GTAC 9 cut(s) 130, 454, 514, 924, 1236, 1484, 1756, 1820, 2035
RsaNI GTAC 9 cut(s) 129, 453, 513, 923, 1235, 1483, 1755, 1819, 2034
SacI GAGCTC 2 cut(s) 277, 450
SatI GCNGC 4 cut(s) 17, 20, 325, 1927
Sau96I GGNCC 6 cut(s) 540, 776, 917, 1211, 1908, 2058
SchI GAGTC 1 cut(s) 1655
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 6 cut(s) 277, 450, 864, 1288, 1362, 1677
SfaNI GCATC 3 cut(s) 642, 1636, 2083
SfcI CTRYAG 3 cut(s) 20, 1245, 1558
SfuI TTCGAA 1 cut(s) 657
SinI GGWCC 2 cut(s) 540, 776
SmlI CTYRAG 6 cut(s) 66, 1190, 1203, 1541, 1841, 1964
SmoI CTYRAG 6 cut(s) 66, 1190, 1203, 1541, 1841, 1964
SseBI AGGCCT 2 cut(s) 1077, 1307
SsiI CCGC 5 cut(s) 396, 422, 538, 779, 1745
SspI AATATT 1 cut(s) 28
SspMI CTAG 5 cut(s) 92, 794, 1109, 1854, 2125
SstI GAGCTC 2 cut(s) 277, 450
StuI AGGCCT 2 cut(s) 1077, 1307
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 5 cut(s) 151, 1288, 1311, 1402, 1992
TaaI ACNGT 5 cut(s) 368, 869, 1297, 1466, 1823
TaiI ACGT 2 cut(s) 935, 1022
TaqI TCGA 9 cut(s) 190, 306, 657, 864, 1118, 1387, 1630, 1953, 2007
TatI WGTACW 2 cut(s) 1818, 2033
TfiI GAWTC 7 cut(s) 436, 706, 1423, 1808, 1955, 1982, 2099
TscAI CASTG 5 cut(s) 130, 274, 606, 631, 1923
TseFI GTSAC 1 cut(s) 1262
TseI GCWGC 4 cut(s) 16, 19, 324, 1926
Tsp45I GTSAC 1 cut(s) 1262
TspDTI ATGAA 5 cut(s) 953, 973, 1493, 1533, 1661
TspGWI ACGGA 3 cut(s) 440, 555, 1946
TspRI CASTG 5 cut(s) 130, 274, 606, 631, 1923
Van91I CCANNNNNTGG 3 cut(s) 237, 719, 1010
Vha464I CTTAAG 1 cut(s) 1964
VpaK11BI GGWCC 2 cut(s) 540, 776
XagI CCTNNNNNAGG 1 cut(s) 1967
XapI RAATTY 2 cut(s) 284, 1392
XceI RCATGY 1 cut(s) 1489
XcmI CCANNNNNNNNNTGG 2 cut(s) 1542, 1747
XspI CTAG 5 cut(s) 92, 794, 1109, 1854, 2125
Zsp2I ATGCAT 1 cut(s) 1786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.