Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0004311

Physical Location & Seq

Genomic Coordinates Forward (+)

32524666 .. 32528632

Gene Structure 3967 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g41340.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGTTGCAGAGGATCTATGGCACTGCATGGGAGAATGAAGAACAATTGCAGGCATACCTTCATTTCAAGGAGGAGGCTAAACGCCGAGATCACCGGCGCCTCGGTCAAGTTCTTGATCTGTTCTCTATACAGAATGATGCTGGTGGAGGTTTGGTGTTCTGGCATCCAAAGGGTGCTATTGTGAGGCATGTAATAGAAGATTTGTGGAAAAAGACACACATAGAGCGTGGTTATGATCTGCTATATACTCCACATGTTGCAAAGGCGGATCTTTGGCAGATAAGTGGTCATCTGGATTTCTACAGAGAAAATATGTACAATCAGATGGATGTTGAAGATGAACTTTATCAACTTCGACCGATGAACTGCCCTTATCATATCTTGATTTACAAAAGGAGGCGTCACTCTTACAATGACTTTCCTATTAGAGTTGCTGAGTTGGGAACAGTATATAGATATGAATTATCTGGAAGCTTACATGGCCTTTTCCGTGTAAGAGGTTTTACTCAGGATGATGCTCACATATTTTGCCTAGAAGACCAGATCAAAGATGAAATCAGGGGTGTCTTAGATCTTACGGAAGAGTTATTATTGAAATTTGGCTTCAGCAAATATGAAGTTAATCTATCCACGAGGCCGGAGAAATCTGTTGGAGACGATGATATATGGGAGAAAGCAACATCTGCCCTTAGAGATGCCTTGGAGGATAAGGGTTGGAGCTATCAAATTGATGATGGTGGTGGTGCTTTTTATGGCCCGAAAATTGATCTTAAGATTGAAGACGCTCTTGGAAGGAAGTGGCAGTGCTCAACTATACAGGTTGATTTTAACCTACCACAGCGATTTGACATAACATATATTGACTCAAATTCAGAAAAGAAGCGGCCTATCATGATTCATAGAGCTGTTCTTGGGTCCTTGGAGCGGTTCTTTGGAGTCCTTATAGAGCATTATGCTGGGGATTTTCCATTATGGCTGTCTCCAGTGCAAGCTCATGTTTTACCAGTGACAGACACTCAGCTGGATTACTGCAAAGAGCTAACCAACAAACTGAAAGTAAGTGGTATTCGGGGTGAACTTTGCCATGGCGAACGACTGCCAAAGTTGATCAGGAATTCAGAGATGCTCAAAATTCCACTAATGGCTGTTGTTGGTGCCAAGGAAGTTGAATCTGGAACTGTTACAGTAAGATCCAGGTCTGGTGACGATCCAGGGACCATGTCGATTGACGATTTTGTCAGTAGAATCAAGTCAGCCATTGAAAGCAGAACATCATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_2b tRNA_SAD

Protein Analysis

427

Amino Acids

49.26

Weight (kDa)

5.95

Isoelectric Point (pI)

46.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_SAD	PF07973	195 - 247	2.7e-12	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	352 - 559	3.5e-49	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	571 - 659	2.1e-17	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04842 AT2G04842
fragaria_vesca	FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340
malus_domestica	MD05G1333800.v1.1 MD09G1120400.v1.1 MD17G1111100.v1.1
prunus_persica	Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1
pyrus_communis	pycom05g23910 pycom05g24220 pycom05g30420 pycom09g04510 pycom15g36000 pycom17g10340
rosa_chinensis	RchiOBHm_Chr2g0156891
rosa_laevigata	RLG00000020950
rosa_multiflora	Rmu_ssc0000408.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092610
rosa_rugosa	Rorug02G0456900
rosa_samantha	Rh2AG522600 Rh2BG535900 Rh2CG507500 Rh2DG544900
rosa_wichuraiana	Rw2G043160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1235
AccB1I	GGYRCC	2 cut(s)	96, 1154
AccBSI	CCGCTC	1 cut(s)	925
AciI	CCGC	3 cut(s)	266, 883, 925
AclWI	GGATC	4 cut(s)	20, 276, 1185, 1202
AcsI	RAATTY	4 cut(s)	596, 868, 1114, 1131
AcuI	CTGAAG	1 cut(s)	589
AcyI	GRCGYC	2 cut(s)	97, 400
AfaI	GTAC	1 cut(s)	317
AfiI	CCNNNNNNNGG	1 cut(s)	924
AflII	CTTAAG	1 cut(s)	770
AflIII	ACRYGT	1 cut(s)	253
AgsI	TTSAA	6 cut(s)	67, 335, 595, 779, 1169, 1262
AjnI	CCWGG	2 cut(s)	1193, 1210
AjuI	GAANNNNNNNTTGG	2 cut(s)	771, 803
AluBI	AGCT	6 cut(s)	474, 720, 905, 992, 1021, 1039
AluI	AGCT	6 cut(s)	474, 720, 905, 992, 1021, 1039
Alw21I	GWGCWC	1 cut(s)	809
Alw26I	GTCTC	2 cut(s)	648, 984
AlwI	GGATC	4 cut(s)	20, 276, 1185, 1202
AoxI	GGCC	4 cut(s)	481, 635, 754, 884
ApoI	RAATTY	4 cut(s)	596, 868, 1114, 1131
AspLEI	GCGC	1 cut(s)	99
AspS9I	GGNCC	3 cut(s)	755, 915, 1215
AsuHPI	GGTGA	3 cut(s)	83, 1085, 1214
AvaII	GGWCC	2 cut(s)	915, 1215
BanI	GGYRCC	2 cut(s)	96, 1154
BauI	CACGAG	1 cut(s)	631
BbsI	GAAGAC	2 cut(s)	543, 786
Bbv12I	GWGCWC	1 cut(s)	809
BccI	CCATC	2 cut(s)	319, 728
BciT130I	CCWGG	2 cut(s)	1195, 1212
BclI	TGATCA	1 cut(s)	1107
BcoDI	GTCTC	2 cut(s)	648, 984
BfaI	CTAG	1 cut(s)	533
BfmI	CTRYAG	1 cut(s)	301
BfoI	RGCGCY	1 cut(s)	100
BfrI	CTTAAG	1 cut(s)	770
BglII	AGATCT	1 cut(s)	571
BisI	GCNGC	1 cut(s)	884
BlsI	GCNGC	1 cut(s)	885
Bme1390I	CCNGG	2 cut(s)	1195, 1212
Bme18I	GGWCC	2 cut(s)	915, 1215
BmgT120I	GGNCC	3 cut(s)	755, 915, 1215
BmiI	GGNNCC	4 cut(s)	98, 916, 1156, 1216
BmrFI	CCNGG	2 cut(s)	1195, 1212
BmsI	GCATC	5 cut(s)	127, 172, 505, 685, 1113
BpiI	GAAGAC	2 cut(s)	543, 786
BpmI	CTGGAG	1 cut(s)	966
BsaHI	GRCGYC	2 cut(s)	97, 400
BsaJI	CCNNGG	6 cut(s)	100, 699, 918, 1084, 1158, 1211
BsaXI	ACNNNNNCTCC	2 cut(s)	138, 168
Bsc4I	CCNNNNNNNGG	1 cut(s)	924
Bse118I	RCCGGY	1 cut(s)	93
Bse1I	ACTGG	2 cut(s)	983, 1004
BseBI	CCWGG	2 cut(s)	1195, 1212
BseDI	CCNNGG	6 cut(s)	100, 699, 918, 1084, 1158, 1211
BseGI	GGATG	3 cut(s)	163, 334, 517
BseLI	CCNNNNNNNGG	1 cut(s)	924
BseMII	CTCAG	3 cut(s)	426, 521, 1031
BseNI	ACTGG	2 cut(s)	983, 1004
BseRI	GAGGAG	1 cut(s)	86
BseYI	CCCAGC	1 cut(s)	956
Bsh1285I	CGRYCG	1 cut(s)	359
BshFI	GGCC	4 cut(s)	483, 637, 756, 886
BshNI	GGYRCC	2 cut(s)	96, 1154
BsiEI	CGRYCG	1 cut(s)	359
BsiHKAI	GWGCWC	1 cut(s)	809
BsiSI	CCGG	2 cut(s)	94, 638
BslFI	GGGAC	1 cut(s)	1228
BslI	CCNNNNNNNGG	1 cut(s)	924
BsmAI	GTCTC	2 cut(s)	648, 984
BsmBI	CGTCTC	1 cut(s)	648
BsmFI	GGGAC	1 cut(s)	1228
BsnI	GGCC	4 cut(s)	483, 637, 756, 886
Bsp1286I	GDGCHC	1 cut(s)	809
Bsp1407I	TGTACA	1 cut(s)	315
Bsp19I	CCATGG	1 cut(s)	1084
BspACI	CCGC	3 cut(s)	266, 883, 925
BspANI	GGCC	4 cut(s)	483, 637, 756, 886
BspCNI	CTCAG	3 cut(s)	427, 520, 1030
BspHI	TCATGA	1 cut(s)	891
BspLI	GGNNCC	4 cut(s)	98, 916, 1156, 1216
BspPI	GGATC	4 cut(s)	20, 276, 1185, 1202
BspT107I	GGYRCC	2 cut(s)	96, 1154
BspTI	CTTAAG	1 cut(s)	770
BsrBI	CCGCTC	1 cut(s)	925
BsrFI	RCCGGY	1 cut(s)	93
BsrGI	TGTACA	1 cut(s)	315
BsrI	ACTGG	2 cut(s)	983, 1004
BssAI	RCCGGY	1 cut(s)	93
BssECI	CCNNGG	6 cut(s)	100, 699, 918, 1084, 1158, 1211
BssNI	GRCGYC	2 cut(s)	97, 400
BssSI	CACGAG	1 cut(s)	631
BssT1I	CCWWGG	4 cut(s)	699, 918, 1084, 1158
Bst2BI	CACGAG	1 cut(s)	631
Bst2UI	CCWGG	2 cut(s)	1195, 1212
Bst4CI	ACNGT	3 cut(s)	448, 1180, 1186
Bst6I	CTCTTC	1 cut(s)	576
BstACI	GRCGYC	2 cut(s)	97, 400
BstAFI	CTTAAG	1 cut(s)	770
BstAPI	GCANNNNNTGC	1 cut(s)	683
BstAUI	TGTACA	1 cut(s)	315
BstC8I	GCNNGC	2 cut(s)	51, 990
BstDEI	CTNAG	5 cut(s)	435, 507, 568, 689, 1017
BstDSI	CCRYGG	1 cut(s)	1084
BstF5I	GGATG	3 cut(s)	163, 334, 517
BstH2I	RGCGCY	1 cut(s)	100
BstHHI	GCGC	1 cut(s)	99
BstMAI	GTCTC	2 cut(s)	648, 984
BstMCI	CGRYCG	1 cut(s)	359
BstMWI	GCNNNNNNNGC	2 cut(s)	480, 683
BstNI	CCWGG	2 cut(s)	1195, 1212
BstNSI	RCATGY	2 cut(s)	191, 257
BstSCI	CCNGG	2 cut(s)	1193, 1210
BstSFI	CTRYAG	1 cut(s)	301
BstV2I	GAAGAC	2 cut(s)	543, 786
BstX2I	RGATCY	4 cut(s)	12, 268, 571, 1190
BstYI	RGATCY	4 cut(s)	12, 268, 571, 1190
BsuRI	GGCC	4 cut(s)	483, 637, 756, 886
BtgI	CCRYGG	1 cut(s)	1084
BtsCI	GGATG	3 cut(s)	163, 334, 517
BtsI	GCAGTG	2 cut(s)	21, 809
BtsIMutI	CAGTG	4 cut(s)	21, 809, 990, 1011
Cac8I	GCNNGC	2 cut(s)	51, 990
CciI	TCATGA	1 cut(s)	891
CfoI	GCGC	1 cut(s)	99
Cfr10I	RCCGGY	1 cut(s)	93
Cfr13I	GGNCC	3 cut(s)	755, 915, 1215
CseI	GACGC	2 cut(s)	389, 791
Csp6I	GTAC	1 cut(s)	316
CviAII	CATG	8 cut(s)	27, 188, 254, 479, 892, 995, 1085, 1219
CviQI	GTAC	1 cut(s)	316
DdeI	CTNAG	5 cut(s)	435, 507, 568, 689, 1017
DinI	GGCGCC	1 cut(s)	98
DrdI	GACNNNNNNGTC	1 cut(s)	1235
DseDI	GACNNNNNNGTC	1 cut(s)	1235
Eam1104I	CTCTTC	1 cut(s)	576
EarI	CTCTTC	1 cut(s)	576
EciI	GGCGGA	1 cut(s)	281
Eco130I	CCWWGG	4 cut(s)	699, 918, 1084, 1158
Eco47I	GGWCC	2 cut(s)	915, 1215
Eco57I	CTGAAG	1 cut(s)	589
EcoO109I	RGGNCCY	1 cut(s)	915
EcoRI	GAATTC	1 cut(s)	1114
EcoRII	CCWGG	2 cut(s)	1193, 1210
EcoT14I	CCWWGG	4 cut(s)	699, 918, 1084, 1158
EgeI	GGCGCC	1 cut(s)	98
EheI	GGCGCC	1 cut(s)	98
ErhI	CCWWGG	4 cut(s)	699, 918, 1084, 1158
Esp3I	CGTCTC	1 cut(s)	648
FaeI	CATG	8 cut(s)	30, 191, 257, 482, 895, 998, 1088, 1222
FalI	AAGNNNNNCTT	4 cut(s)	327, 359, 771, 803
FaqI	GGGAC	1 cut(s)	1228
FatI	CATG	8 cut(s)	26, 187, 253, 478, 891, 994, 1084, 1218
FbaI	TGATCA	1 cut(s)	1107
Fnu4HI	GCNGC	1 cut(s)	884
FokI	GGATG	3 cut(s)	150, 341, 524
Fsp4HI	GCNGC	1 cut(s)	884
FspBI	CTAG	1 cut(s)	533
GlaI	GCGC	1 cut(s)	98
GluI	GCNGC	1 cut(s)	884
GsaI	CCCAGC	1 cut(s)	960
GsuI	CTGGAG	1 cut(s)	966
HaeII	RGCGCY	1 cut(s)	100
HaeIII	GGCC	4 cut(s)	483, 637, 756, 886
HapII	CCGG	2 cut(s)	94, 638
HgaI	GACGC	2 cut(s)	389, 791
HhaI	GCGC	1 cut(s)	99
Hin1I	GRCGYC	2 cut(s)	97, 400
Hin1II	CATG	8 cut(s)	30, 191, 257, 482, 895, 998, 1088, 1222
Hin6I	GCGC	1 cut(s)	97
HinP1I	GCGC	1 cut(s)	97
HindIII	AAGCTT	1 cut(s)	472
HinfI	GANTC	5 cut(s)	863, 895, 936, 1169, 1245
HpaII	CCGG	2 cut(s)	94, 638
HphI	GGTGA	3 cut(s)	83, 1085, 1214
Hpy166II	GTNNAC	1 cut(s)	1076
Hpy188I	TCNGA	3 cut(s)	324, 874, 1120
Hpy188III	TCNNGA	8 cut(s)	113, 293, 382, 468, 509, 892, 1111, 1173
Hpy8I	GTNNAC	1 cut(s)	1076
HpyAV	CCTTC	2 cut(s)	68, 786
HpyCH4III	ACNGT	3 cut(s)	448, 1180, 1186
HpyCH4V	TGCA	6 cut(s)	7, 26, 49, 260, 988, 1032
HpyF10VI	GCNNNNNNNGC	2 cut(s)	480, 683
HpyF3I	CTNAG	5 cut(s)	435, 507, 568, 689, 1017
Hsp92I	GRCGYC	2 cut(s)	97, 400
Hsp92II	CATG	8 cut(s)	30, 191, 257, 482, 895, 998, 1088, 1222
HspAI	GCGC	1 cut(s)	97
KasI	GGCGCC	1 cut(s)	96
Ksp22I	TGATCA	1 cut(s)	1107
LmnI	GCTCC	2 cut(s)	717, 922
LweI	GCATC	5 cut(s)	127, 172, 505, 685, 1113
MaeI	CTAG	1 cut(s)	533
MaeIII	GTNAC	4 cut(s)	401, 1006, 1180, 1202
MbiI	CCGCTC	1 cut(s)	925
MboII	GAAGA	6 cut(s)	50, 209, 347, 548, 593, 791
MfeI	CAATTG	1 cut(s)	44
MflI	RGATCY	4 cut(s)	12, 268, 571, 1190
MhlI	GDGCHC	1 cut(s)	809
MluCI	AATT	8 cut(s)	44, 461, 596, 726, 762, 868, 1114, 1131
Mly113I	GGCGCC	1 cut(s)	97
MlyI	GAGTC	2 cut(s)	857, 945
MmeI	TCCRAC	2 cut(s)	631, 695
MseI	TTAA	3 cut(s)	621, 771, 828
MspA1I	CMGCKG	1 cut(s)	1021
MspCI	CTTAAG	1 cut(s)	770
MspI	CCGG	2 cut(s)	94, 638
MspR9I	CCNGG	2 cut(s)	1195, 1212
MunI	CAATTG	1 cut(s)	44
MvaI	CCWGG	2 cut(s)	1195, 1212
MwoI	GCNNNNNNNGC	2 cut(s)	480, 683
NarI	GGCGCC	1 cut(s)	97
NcoI	CCATGG	1 cut(s)	1084
NlaIII	CATG	8 cut(s)	30, 191, 257, 482, 895, 998, 1088, 1222
NlaIV	GGNNCC	4 cut(s)	98, 916, 1156, 1216
NmeAIII	GCCGAG	1 cut(s)	110
NmuCI	GTSAC	3 cut(s)	401, 1006, 1202
NspI	RCATGY	2 cut(s)	191, 257
PagI	TCATGA	1 cut(s)	891
PciI	ACATGT	1 cut(s)	253
PfeI	GAWTC	3 cut(s)	895, 1169, 1245
PflFI	GACNNNGTC	1 cut(s)	1219
PkrI	GCNGC	1 cut(s)	885
PleI	GAGTC	2 cut(s)	857, 944
PluTI	GGCGCC	1 cut(s)	100
PpsI	GAGTC	2 cut(s)	857, 944
PpuMI	RGGWCCY	1 cut(s)	915
PscI	ACATGT	1 cut(s)	253
Psp5II	RGGWCCY	1 cut(s)	915
Psp6I	CCWGG	2 cut(s)	1193, 1210
PspFI	CCCAGC	1 cut(s)	956
PspGI	CCWGG	2 cut(s)	1193, 1210
PspN4I	GGNNCC	4 cut(s)	98, 916, 1156, 1216
PspPI	GGNCC	3 cut(s)	755, 915, 1215
PspPPI	RGGWCCY	1 cut(s)	915
PsuI	RGATCY	4 cut(s)	12, 268, 571, 1190
PsyI	GACNNNGTC	1 cut(s)	1219
PvuII	CAGCTG	1 cut(s)	1021
RsaI	GTAC	1 cut(s)	317
RsaNI	GTAC	1 cut(s)	316
SaqAI	TTAA	3 cut(s)	621, 771, 828
SatI	GCNGC	1 cut(s)	884
Sau96I	GGNCC	3 cut(s)	755, 915, 1215
SchI	GAGTC	2 cut(s)	857, 945
ScrFI	CCNGG	2 cut(s)	1195, 1212
SduI	GDGCHC	1 cut(s)	809
SfaNI	GCATC	5 cut(s)	127, 172, 505, 685, 1113
SfcI	CTRYAG	1 cut(s)	301
SfoI	GGCGCC	1 cut(s)	98
SgrAI	CRCCGGYG	1 cut(s)	93
SinI	GGWCC	2 cut(s)	915, 1215
SmlI	CTYRAG	1 cut(s)	770
SmoI	CTYRAG	1 cut(s)	770
Sse9I	AATT	8 cut(s)	44, 461, 596, 726, 762, 868, 1114, 1131
SsiI	CCGC	3 cut(s)	266, 883, 925
SspDI	GGCGCC	1 cut(s)	96
SspMI	CTAG	1 cut(s)	533
StyD4I	CCNGG	2 cut(s)	1193, 1210
StyI	CCWWGG	4 cut(s)	699, 918, 1084, 1158
TaaI	ACNGT	3 cut(s)	448, 1180, 1186
TaqI	TCGA	2 cut(s)	355, 1223
TaqII	GACCGA	2 cut(s)	92, 373
TasI	AATT	8 cut(s)	44, 461, 596, 726, 762, 868, 1114, 1131
TatI	WGTACW	1 cut(s)	315
TauI	GCSGC	1 cut(s)	886
TfiI	GAWTC	3 cut(s)	895, 1169, 1245
Tru1I	TTAA	3 cut(s)	621, 771, 828
Tru9I	TTAA	3 cut(s)	621, 771, 828
TscAI	CASTG	4 cut(s)	28, 809, 990, 1011
TseFI	GTSAC	3 cut(s)	401, 1006, 1202
Tsp45I	GTSAC	3 cut(s)	401, 1006, 1202
TspDTI	ATGAA	8 cut(s)	50, 51, 354, 377, 474, 567, 630, 887
TspGWI	ACGGA	2 cut(s)	479, 593
TspRI	CASTG	4 cut(s)	28, 809, 990, 1011
Tth111I	GACNNNGTC	1 cut(s)	1219
Vha464I	CTTAAG	1 cut(s)	770
VpaK11BI	GGWCC	2 cut(s)	915, 1215
XapI	RAATTY	4 cut(s)	596, 868, 1114, 1131
XceI	RCATGY	2 cut(s)	191, 257
XspI	CTAG	1 cut(s)	533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.