Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004311

Physical Location & Seq

Genomic Coordinates Reverse (-)

9546096 .. 9547699

Gene Structure 1604 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1111100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 813 bp

ATGTATGATCAAATGAATGTTGAGGATGGACTATATCAGCTTCGACCAATGAACTGCCCCTATCATATCTTGGTTTACAAAAGGAAGCCTCACTCATATCATGAATTTCCTATTCGAGTTGCTGAGTTGGGAACCGTATATAGATACGAATTATCTGGAAGCTTGCATGGTCTTTTTCGTGATGATGCCCACATATTTTGTCTAGAAGATCAGATCAAAGATGAAATCAGGGGTGTCCTAGATCTTACCGAAGAACTTTTGTTGCAATTTGGCTTCAACAAATATGAGGTCAATCTCTGTACGAGGCCAGAGAAAGCTGTTGGAGATGATGATATATGGGCGAAAGCAACATCTTCCCTTAGAGATGCTTTGGATGATAAAGGTTGGAGCTATCAAATTGATGAAGGTGCTGGTGCCTTTTATGGTCCAAAGATTGATCTTAAGATTGAGGATGCCCTTGGAAGGAATCTCTCTGTTGATTTTAACCTACCACAGCGTTTTGACATAACATATGTTGACTCAAACTCTGAAAAGAAGCGGCCTATCATGATTCATAGAGCTGTTCTTGGGTCATTGGAGCGGTTTTTTGGGGTCCTTATAGAGCATTATGCCGGGGATTTTCCATTATGGCTTTCACCAGTACAAGCTCATGTTGTACCAGTTACTGACTCCCAGCTTGATTACTGCAAAGAGGTAACCAACAAACTGAAAGCAAATGGTATTCGTGGTGAACTTTGCCAGGGTGAACGCCTGCCGAAACTGATTAGAAATTCAGAGATACAGAAAATTCCATTGATGGCTGTTGGAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_2b tRNA_SAD

Protein Analysis

271

Amino Acids

30.95

Weight (kDa)

5.42

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	11 - 203	5.2e-34	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	215 - 268	2.8e-06	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04842 AT2G04842
fragaria_vesca	FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340
malus_domestica	MD05G1333800.v1.1 MD09G1120400.v1.1 MD17G1111100.v1.1
prunus_persica	Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1
pyrus_communis	pycom05g23910 pycom05g24220 pycom05g30420 pycom09g04510 pycom15g36000 pycom17g10340
rosa_chinensis	RchiOBHm_Chr2g0156891
rosa_laevigata	RLG00000020950
rosa_multiflora	Rmu_ssc0000408.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092610
rosa_rugosa	Rorug02G0456900
rosa_samantha	Rh2AG522600 Rh2BG535900 Rh2CG507500 Rh2DG544900
rosa_wichuraiana	Rw2G043160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	413
AccBSI	CCGCTC	1 cut(s)	580
AciI	CCGC	2 cut(s)	538, 580
AcsI	RAATTY	3 cut(s)	104, 769, 786
AfaI	GTAC	3 cut(s)	301, 642, 657
AfiI	CCNNNNNNNGG	1 cut(s)	462
AflII	CTTAAG	1 cut(s)	440
AgsI	TTSAA	1 cut(s)	277
AjnI	CCWGG	1 cut(s)	738
AluBI	AGCT	7 cut(s)	40, 162, 317, 390, 560, 647, 676
AluI	AGCT	7 cut(s)	40, 162, 317, 390, 560, 647, 676
AlwNI	CAGNNNCTG	1 cut(s)	665
AoxI	GGCC	2 cut(s)	305, 539
ApoI	RAATTY	3 cut(s)	104, 769, 786
ArsI	GACNNNNNNTTYG	2 cut(s)	273, 305
AspS9I	GGNCC	2 cut(s)	425, 592
AsuC2I	CCSGG	1 cut(s)	613
AsuHPI	GGTGA	3 cut(s)	627, 740, 755
AvaII	GGWCC	2 cut(s)	425, 592
BanI	GGYRCC	1 cut(s)	413
BccI	CCATC	2 cut(s)	20, 790
BciT130I	CCWGG	1 cut(s)	740
BclI	TGATCA	1 cut(s)	7
BcnI	CCSGG	1 cut(s)	613
BfaI	CTAG	2 cut(s)	203, 239
BfrI	CTTAAG	1 cut(s)	440
BglII	AGATCT	1 cut(s)	241
BisI	GCNGC	1 cut(s)	539
BlsI	GCNGC	1 cut(s)	540
Bme1390I	CCNGG	2 cut(s)	613, 740
Bme18I	GGWCC	2 cut(s)	425, 592
BmgT120I	GGNCC	2 cut(s)	425, 592
BmiI	GGNNCC	3 cut(s)	133, 415, 593
BmrFI	CCNGG	2 cut(s)	613, 740
BmsI	GCATC	3 cut(s)	175, 355, 442
BpuMI	CCSGG	1 cut(s)	613
BsaJI	CCNNGG	3 cut(s)	457, 612, 739
Bsc4I	CCNNNNNNNGG	1 cut(s)	462
Bse1I	ACTGG	2 cut(s)	638, 659
BseBI	CCWGG	1 cut(s)	740
BseDI	CCNNGG	3 cut(s)	457, 612, 739
BseGI	GGATG	3 cut(s)	31, 379, 457
BseLI	CCNNNNNNNGG	1 cut(s)	462
BseMII	CTCAG	1 cut(s)	114
BseNI	ACTGG	2 cut(s)	638, 659
BseYI	CCCAGC	1 cut(s)	672
BshFI	GGCC	2 cut(s)	307, 541
BshNI	GGYRCC	1 cut(s)	413
BsiSI	CCGG	1 cut(s)	612
BslI	CCNNNNNNNGG	1 cut(s)	462
BsnI	GGCC	2 cut(s)	307, 541
Bsp143I	GATC	5 cut(s)	7, 208, 213, 241, 436
BspACI	CCGC	2 cut(s)	538, 580
BspANI	GGCC	2 cut(s)	307, 541
BspCNI	CTCAG	1 cut(s)	115
BspHI	TCATGA	2 cut(s)	100, 546
BspLI	GGNNCC	3 cut(s)	133, 415, 593
BspT107I	GGYRCC	1 cut(s)	413
BspTI	CTTAAG	1 cut(s)	440
BsrBI	CCGCTC	1 cut(s)	580
BsrI	ACTGG	2 cut(s)	638, 659
BssECI	CCNNGG	3 cut(s)	457, 612, 739
BssMI	GATC	5 cut(s)	7, 208, 213, 241, 436
BssT1I	CCWWGG	1 cut(s)	457
Bst2UI	CCWGG	1 cut(s)	740
Bst4CI	ACNGT	1 cut(s)	136
BstAFI	CTTAAG	1 cut(s)	440
BstC8I	GCNNGC	2 cut(s)	164, 752
BstDEI	CTNAG	2 cut(s)	123, 359
BstEII	GGTNACC	1 cut(s)	694
BstF5I	GGATG	3 cut(s)	31, 379, 457
BstKTI	GATC	5 cut(s)	10, 211, 216, 244, 439
BstMBI	GATC	5 cut(s)	7, 208, 213, 241, 436
BstNI	CCWGG	1 cut(s)	740
BstPI	GGTNACC	1 cut(s)	694
BstSCI	CCNGG	2 cut(s)	611, 738
BstX2I	RGATCY	1 cut(s)	241
BstYI	RGATCY	1 cut(s)	241
BsuRI	GGCC	2 cut(s)	307, 541
BtsCI	GGATG	3 cut(s)	31, 379, 457
Cac8I	GCNNGC	2 cut(s)	164, 752
CaiI	CAGNNNCTG	1 cut(s)	665
CciI	TCATGA	2 cut(s)	100, 546
Cfr13I	GGNCC	2 cut(s)	425, 592
Csp6I	GTAC	3 cut(s)	300, 641, 656
CspCI	CAANNNNNGTGG	2 cut(s)	179, 214
CviAII	CATG	4 cut(s)	101, 167, 547, 650
CviQI	GTAC	3 cut(s)	300, 641, 656
DdeI	CTNAG	2 cut(s)	123, 359
DpnI	GATC	5 cut(s)	9, 210, 215, 243, 438
DpnII	GATC	5 cut(s)	7, 208, 213, 241, 436
Eco130I	CCWWGG	1 cut(s)	457
Eco47I	GGWCC	2 cut(s)	425, 592
Eco91I	GGTNACC	1 cut(s)	694
EcoO109I	RGGNCCY	1 cut(s)	592
EcoO65I	GGTNACC	1 cut(s)	694
EcoRII	CCWGG	1 cut(s)	738
EcoT14I	CCWWGG	1 cut(s)	457
ErhI	CCWWGG	1 cut(s)	457
FaeI	CATG	4 cut(s)	104, 170, 550, 653
FatI	CATG	4 cut(s)	100, 166, 546, 649
FauNDI	CATATG	1 cut(s)	511
FbaI	TGATCA	1 cut(s)	7
Fnu4HI	GCNGC	1 cut(s)	539
FokI	GGATG	3 cut(s)	38, 386, 464
Fsp4HI	GCNGC	1 cut(s)	539
FspBI	CTAG	2 cut(s)	203, 239
GluI	GCNGC	1 cut(s)	539
GsaI	CCCAGC	1 cut(s)	676
HaeIII	GGCC	2 cut(s)	307, 541
HapII	CCGG	1 cut(s)	612
Hin1II	CATG	4 cut(s)	104, 170, 550, 653
HincII	GTYRAC	1 cut(s)	517
HindII	GTYRAC	1 cut(s)	517
HindIII	AAGCTT	1 cut(s)	160
HinfI	GANTC	5 cut(s)	466, 518, 550, 668, 807
HpaII	CCGG	1 cut(s)	612
HphI	GGTGA	3 cut(s)	627, 740, 755
Hpy166II	GTNNAC	4 cut(s)	76, 517, 731, 746
Hpy188I	TCNGA	3 cut(s)	213, 529, 775
Hpy188III	TCNNGA	5 cut(s)	101, 156, 179, 203, 547
Hpy8I	GTNNAC	4 cut(s)	76, 517, 731, 746
HpyAV	CCTTC	2 cut(s)	398, 456
HpyCH4III	ACNGT	1 cut(s)	136
HpyCH4V	TGCA	3 cut(s)	166, 265, 687
HpyF3I	CTNAG	2 cut(s)	123, 359
Hsp92II	CATG	4 cut(s)	104, 170, 550, 653
Ksp22I	TGATCA	1 cut(s)	7
Kzo9I	GATC	5 cut(s)	7, 208, 213, 241, 436
LmnI	GCTCC	2 cut(s)	387, 577
LweI	GCATC	3 cut(s)	175, 355, 442
MaeI	CTAG	2 cut(s)	203, 239
MaeIII	GTNAC	2 cut(s)	661, 694
MalI	GATC	5 cut(s)	9, 210, 215, 243, 438
MbiI	CCGCTC	1 cut(s)	580
MboI	GATC	5 cut(s)	7, 208, 213, 241, 436
MboII	GAAGA	3 cut(s)	218, 263, 345
MflI	RGATCY	1 cut(s)	241
MluCI	AATT	6 cut(s)	104, 149, 266, 396, 769, 786
MlyI	GAGTC	2 cut(s)	512, 662
MmeI	TCCRAC	3 cut(s)	301, 365, 784
MnlI	CCTC	6 cut(s)	16, 99, 280, 297, 442, 685
MseI	TTAA	2 cut(s)	441, 483
MspCI	CTTAAG	1 cut(s)	440
MspI	CCGG	1 cut(s)	612
MspR9I	CCNGG	2 cut(s)	613, 740
MvaI	CCWGG	1 cut(s)	740
NciI	CCSGG	1 cut(s)	613
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	5 cut(s)	7, 208, 213, 241, 436
NlaIII	CATG	4 cut(s)	104, 170, 550, 653
NlaIV	GGNNCC	3 cut(s)	133, 415, 593
PagI	TCATGA	2 cut(s)	100, 546
PfeI	GAWTC	3 cut(s)	466, 550, 807
PkrI	GCNGC	1 cut(s)	540
PleI	GAGTC	2 cut(s)	512, 662
PpsI	GAGTC	2 cut(s)	512, 662
PpuMI	RGGWCCY	1 cut(s)	592
Psp5II	RGGWCCY	1 cut(s)	592
Psp6I	CCWGG	1 cut(s)	738
PspEI	GGTNACC	1 cut(s)	694
PspFI	CCCAGC	1 cut(s)	672
PspGI	CCWGG	1 cut(s)	738
PspN4I	GGNNCC	3 cut(s)	133, 415, 593
PspPI	GGNCC	2 cut(s)	425, 592
PspPPI	RGGWCCY	1 cut(s)	592
PstNI	CAGNNNCTG	1 cut(s)	665
PsuI	RGATCY	1 cut(s)	241
RsaI	GTAC	3 cut(s)	301, 642, 657
RsaNI	GTAC	3 cut(s)	300, 641, 656
SaqAI	TTAA	2 cut(s)	441, 483
SatI	GCNGC	1 cut(s)	539
Sau3AI	GATC	5 cut(s)	7, 208, 213, 241, 436
Sau96I	GGNCC	2 cut(s)	425, 592
SchI	GAGTC	2 cut(s)	512, 662
ScrFI	CCNGG	2 cut(s)	613, 740
SfaNI	GCATC	3 cut(s)	175, 355, 442
SinI	GGWCC	2 cut(s)	425, 592
SmlI	CTYRAG	1 cut(s)	440
SmoI	CTYRAG	1 cut(s)	440
Sse9I	AATT	6 cut(s)	104, 149, 266, 396, 769, 786
SsiI	CCGC	2 cut(s)	538, 580
SspMI	CTAG	2 cut(s)	203, 239
StyD4I	CCNGG	2 cut(s)	611, 738
StyI	CCWWGG	1 cut(s)	457
TaaI	ACNGT	1 cut(s)	136
TaqI	TCGA	2 cut(s)	43, 115
TasI	AATT	6 cut(s)	104, 149, 266, 396, 769, 786
TatI	WGTACW	1 cut(s)	640
TauI	GCSGC	1 cut(s)	541
TfiI	GAWTC	3 cut(s)	466, 550, 807
Tru1I	TTAA	2 cut(s)	441, 483
Tru9I	TTAA	2 cut(s)	441, 483
TspDTI	ATGAA	6 cut(s)	29, 65, 117, 237, 417, 542
Vha464I	CTTAAG	1 cut(s)	440
VpaK11BI	GGWCC	2 cut(s)	425, 592
XapI	RAATTY	3 cut(s)	104, 769, 786
XbaI	TCTAGA	1 cut(s)	202
XspI	CTAG	2 cut(s)	203, 239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.