Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004311

Physical Location & Seq

Genomic Coordinates Reverse (-)

9318907 .. 9322973

Gene Structure 4067 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1120400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGTTAATTCTTCAGAGAATGGCAATGGCCACTTCTTCTTCCTTCCTCTCAATCCCTCTCCTGAAGACCCCCCCTTCTTTCCTTTCCCCGATTAAACACTGCGTTTCGCCACTGAACTCCGAGTTCAGGGCTCTGGCCCTCTACGGCGGCAGAAATGGAGTCTCCACCTCCTCAGCAGTGGCCACGGAGGCTCAGGTCTCGACCCAAGACGACAAATTGAAGGATGCCCAGATGGAGGTAACTGATAAGCCTGAGAAAGTCGTGCTTCCAACCAACGAGTCCTCGGAGAGCCTGCTCAGAATTCGACACACGTGTGCACACGTGATGGCCATGGCTGTTCAAAAGCTCTTCCCGGATGCAAAAGTGACAATTGGTCCGTGGATAGAAAATGGGTTCTATTATGATTTTGATATGGAGCCTTTGACGGACAAAGAGTTGAAGAGGATCAAGAAGGAAATGGATCGCATCATTGGTAGGAATTTACCACTTATAAGAGAGGAAGTTTCAAGAGATGAAGCTCACAGAAGAATAACTGCTCTCAACGAACCATACAAATTGGAAATTTTGAATAGTATTAAGGAAGATCCCATTACCATATATCACATTGGCGATGAATGGTGGGACCTGTGTGCTGGGCCTCATGTTGAAAAAACTGGAAAAATTAACAGAAAAGCTGTCGAACTTGAGTCTATTGCTGGTGCTTACTGGAGAGGTGATGAAAAGAAACCCATGCTGCAGAGGATTTATGGCACTGCATGGGAGAGCGAAGAACAATTGAAGGCATATCTTCATTTCAAAGCGGAAGCTAAACGTAGAGATCACAGGCGCCTTGGTCAAGTTCTTGATCTGTTTTCTATACAGAATGATGCCGGTGGGGGTTTAGTGTTCTGGCATCCAAAGGGTGCTATTGTGAGGCATGTAATAGAAGATTTGTGGAAAAAGATCCACATAGAATGTGGTTATGATTTGCTGTATACTCCACATGTTGCAAAGGCAAACCTTTGGCAGATAAGTGGTCATCTGGATTATTACAGAGAGAATATGTATGATCAGATGAATGTTGAGGATGAACTTTATCAGCTTCGACCGATGAACTGCCCCTATCATATCTTGGTTTACAAAAGAAAGCGTCACTCGTATCATGAATTCCCTATTCGAGTTGCTGAGTTGGGAACCGTATATAGGTATGAGTTATCTGGAAGCTTGCATGGCCTTTTTCGTGTAAGAGGTTTTACTCAGGATGATGCACACATATTTTGTCTAGAAGATCAGATCAAAGATGAAATCAGGGGTGTCCTAGATCTTACCGAAGAACTTTTGTTGCAATTTGGCTTCAGTAATTATGAAGTCAATCTATCTACGAGGCCAGAGAAAGCTGTTGGAGATGATGATATATGGGTGAAAGCAACATCTGCCCTTAGAGATGCTTTGGATGATAAAGGTTGGAGCTATCAAATTGATGAAGGTGGTGGTGCCTTTTATGGTCCAAAGATTGATCTTAAGATTGAGGATGCCCTTGGAAGGAAGTGGCAGTGCTCAACTATACAGGTTGATTTTAACCTACCACAGCGTTTTGACATAACATATGTTGACTCAAACTCGGAAAAGAAGCGGCCTATCATGATCCATAGAGCTGTTCTTGGGTCATTGGAGAGATTCTTTGGAGTCCTTATAGAGCATTATGCGGGGGATTTTCCATTATGGCTTTCGCCAGTGCAAGCTCATGTTTTACCAGTTACCGACACTCAGCTTGATTACTGCAAAGAGGTAACCAACAAACTGAAAGCAAATGGTATCCGCGGCGAACTTTGCCAGGGTGAACGCCTTCCAAAACTGATTAGAAATTCAGAGATGCAGAAAATTCCATTAATGGCTGTTGTCGGTGCCAAGGAAGTTGAAACTCGCACTGTTACAGTAAGATCCAGGTTTGGTGGCGATCTTGGCACCATGCCAATTGATGACTTTGTCAGTACAATCAAGTCGGCCATTGAAAGCAAAGCGTCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_2b tRNA_SAD

Protein Analysis

670

Amino Acids

76.52

Weight (kDa)

6.2

Isoelectric Point (pI)

40.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_SAD	PF07973	197 - 249	5.2e-13	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	356 - 561	1.6e-48	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	573 - 661	5.3e-19	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04842 AT2G04842
fragaria_vesca	FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340 FvH4_6g41340
malus_domestica	MD05G1333800.v1.1 MD09G1120400.v1.1 MD17G1111100.v1.1
prunus_persica	Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1 Prupe.3G203100_v2.0.a1
pyrus_communis	pycom05g23910 pycom05g24220 pycom05g30420 pycom09g04510 pycom15g36000 pycom17g10340
rosa_chinensis	RchiOBHm_Chr2g0156891
rosa_laevigata	RLG00000020950
rosa_multiflora	Rmu_ssc0000408.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092610
rosa_rugosa	Rorug02G0456900
rosa_samantha	Rh2AG522600 Rh2BG535900 Rh2CG507500 Rh2DG544900
rosa_wichuraiana	Rw2G043160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	491
AccB1I	GGYRCC	4 cut(s)	825, 1472, 1883, 1943
AccI	GTMKAC	1 cut(s)	974
AccII	CGCG	1 cut(s)	1800
AciI	CCGC	6 cut(s)	147, 800, 1612, 1685, 1798, 1800
AclWI	GGATC	6 cut(s)	452, 468, 578, 937, 1618, 1914
AcoI	YGGCCR	4 cut(s)	27, 180, 327, 1983
AcsI	RAATTY	6 cut(s)	300, 478, 561, 1145, 1843, 1860
AcuI	CTGAAG	2 cut(s)	83, 1318
AcvI	CACGTG	2 cut(s)	312, 322
AcyI	GRCGYC	1 cut(s)	826
AfaI	GTAC	1 cut(s)	1972
AfiI	CCNNNNNNNGG	4 cut(s)	126, 235, 1182, 1521
AflII	CTTAAG	1 cut(s)	1499
AflIII	ACRYGT	4 cut(s)	309, 311, 319, 982
AhdI	GACNNNNNGTC	1 cut(s)	372
AjnI	CCWGG	2 cut(s)	1812, 1922
AleI	CACNNNNGTG	1 cut(s)	312
Alw21I	GWGCWC	2 cut(s)	319, 1538
Alw26I	GTCTC	2 cut(s)	166, 202
Alw44I	GTGCAC	1 cut(s)	315
AlwI	GGATC	6 cut(s)	452, 468, 578, 937, 1618, 1914
AoxI	GGCC	9 cut(s)	27, 135, 180, 327, 635, 1210, 1364, 1613, 1983
ApaLI	GTGCAC	1 cut(s)	315
ApeKI	GCWGC	1 cut(s)	733
ApoI	RAATTY	6 cut(s)	300, 478, 561, 1145, 1843, 1860
AseI	ATTAAT	1 cut(s)	1868
Asp700I	GAANNNNTTC	1 cut(s)	1824
AspLEI	GCGC	1 cut(s)	828
AspS9I	GGNCC	5 cut(s)	136, 374, 622, 635, 1484
AsuC2I	CCSGG	1 cut(s)	353
AsuHPI	GGTGA	3 cut(s)	725, 1411, 1829
AvaII	GGWCC	3 cut(s)	374, 622, 1484
BaeGI	GKGCMC	1 cut(s)	319
BalI	TGGCCA	3 cut(s)	29, 182, 329
BanI	GGYRCC	4 cut(s)	825, 1472, 1883, 1943
BanII	GRGCYC	1 cut(s)	133
BbrPI	CACGTG	2 cut(s)	312, 322
BbsI	GAAGAC	1 cut(s)	71
Bbv12I	GWGCWC	2 cut(s)	319, 1538
BbvCI	CCTCAGC	1 cut(s)	172
BbvI	GCAGC	1 cut(s)	720
BccI	CCATC	2 cut(s)	226, 319
BceAI	ACGGC	1 cut(s)	160
BciT130I	CCWGG	2 cut(s)	1814, 1924
BciVI	GTATCC	1 cut(s)	1805
BclI	TGATCA	1 cut(s)	1048
BcnI	CCSGG	1 cut(s)	353
BcoDI	GTCTC	2 cut(s)	166, 202
BfaI	CTAG	2 cut(s)	1262, 1298
BfmI	CTRYAG	1 cut(s)	734
BfoI	RGCGCY	1 cut(s)	829
BfrI	CTTAAG	1 cut(s)	1499
BfuI	GTATCC	1 cut(s)	1805
BglI	GCCNNNNNGGC	1 cut(s)	188
BglII	AGATCT	1 cut(s)	1300
BisI	GCNGC	4 cut(s)	148, 734, 1613, 1801
BlsI	GCNGC	4 cut(s)	149, 735, 1614, 1802
Bme1390I	CCNGG	3 cut(s)	353, 1814, 1924
Bme18I	GGWCC	3 cut(s)	374, 622, 1484
BmeRI	GACNNNNNGTC	1 cut(s)	372
BmgT120I	GGNCC	5 cut(s)	136, 374, 622, 635, 1484
BmiI	GGNNCC	7 cut(s)	417, 623, 827, 1174, 1474, 1885, 1945
BmrFI	CCNGG	3 cut(s)	353, 1814, 1924
BmsI	GCATC	9 cut(s)	214, 346, 474, 856, 901, 1234, 1414, 1501, 1842
BpiI	GAAGAC	1 cut(s)	71
BpmI	CTGGAG	1 cut(s)	727
Bpu10I	CCTNAGC	2 cut(s)	172, 192
BpuEI	CTTGAG	1 cut(s)	704
BpuMI	CCSGG	1 cut(s)	353
BsaAI	YACGTR	2 cut(s)	312, 322
BsaHI	GRCGYC	1 cut(s)	826
BsaI	GGTCTC	1 cut(s)	202
BsaJI	CCNNGG	9 cut(s)	183, 282, 330, 377, 829, 1516, 1798, 1813, 1887
Bsc4I	CCNNNNNNNGG	4 cut(s)	126, 235, 1182, 1521
Bse118I	RCCGGY	1 cut(s)	869
Bse1I	ACTGG	4 cut(s)	658, 710, 1712, 1733
Bse3DI	GCAATG	1 cut(s)	30
BseBI	CCWGG	2 cut(s)	1814, 1924
BseDI	CCNNGG	9 cut(s)	183, 282, 330, 377, 829, 1516, 1798, 1813, 1887
BseGI	GGATG	7 cut(s)	229, 361, 892, 1072, 1246, 1438, 1516
BseLI	CCNNNNNNNGG	4 cut(s)	126, 235, 1182, 1521
BseMI	GCAATG	1 cut(s)	30
BseMII	CTCAG	7 cut(s)	186, 206, 243, 310, 1155, 1250, 1760
BseNI	ACTGG	4 cut(s)	658, 710, 1712, 1733
BseRI	GAGGAG	1 cut(s)	160
BseSI	GKGCMC	1 cut(s)	319
BseXI	GCAGC	1 cut(s)	720
BseYI	CCCAGC	1 cut(s)	632
Bsh1236I	CGCG	1 cut(s)	1800
Bsh1285I	CGRYCG	1 cut(s)	1088
BshFI	GGCC	9 cut(s)	29, 137, 182, 329, 637, 1212, 1366, 1615, 1985
BshNI	GGYRCC	4 cut(s)	825, 1472, 1883, 1943
BsiEI	CGRYCG	1 cut(s)	1088
BsiHKAI	GWGCWC	2 cut(s)	319, 1538
BsiSI	CCGG	2 cut(s)	353, 870
BslFI	GGGAC	1 cut(s)	635
BslI	CCNNNNNNNGG	4 cut(s)	126, 235, 1182, 1521
BsmAI	GTCTC	2 cut(s)	166, 202
BsmFI	GGGAC	1 cut(s)	635
BsnI	GGCC	9 cut(s)	29, 137, 182, 329, 637, 1212, 1366, 1615, 1985
Bso31I	GGTCTC	1 cut(s)	202
Bsp1286I	GDGCHC	3 cut(s)	133, 319, 1538
Bsp19I	CCATGG	1 cut(s)	330
BspACI	CCGC	6 cut(s)	147, 800, 1612, 1685, 1798, 1800
BspANI	GGCC	9 cut(s)	29, 137, 182, 329, 637, 1212, 1366, 1615, 1985
BspCNI	CTCAG	7 cut(s)	185, 205, 244, 309, 1156, 1249, 1759
BspFNI	CGCG	1 cut(s)	1800
BspHI	TCATGA	2 cut(s)	1141, 1620
BspLI	GGNNCC	7 cut(s)	417, 623, 827, 1174, 1474, 1885, 1945
BspMAI	CTGCAG	1 cut(s)	738
BspPI	GGATC	6 cut(s)	452, 468, 578, 937, 1618, 1914
BspQI	GCTCTTC	1 cut(s)	353
BspT107I	GGYRCC	4 cut(s)	825, 1472, 1883, 1943
BspTI	CTTAAG	1 cut(s)	1499
BspTNI	GGTCTC	1 cut(s)	202
BsrDI	GCAATG	1 cut(s)	30
BsrFI	RCCGGY	1 cut(s)	869
BsrI	ACTGG	4 cut(s)	658, 710, 1712, 1733
BssAI	RCCGGY	1 cut(s)	869
BssECI	CCNNGG	9 cut(s)	183, 282, 330, 377, 829, 1516, 1798, 1813, 1887
BssNAI	GTATAC	1 cut(s)	975
BssNI	GRCGYC	1 cut(s)	826
BssT1I	CCWWGG	4 cut(s)	330, 829, 1516, 1887
Bst1107I	GTATAC	1 cut(s)	975
Bst2UI	CCWGG	2 cut(s)	1814, 1924
Bst4CI	ACNGT	3 cut(s)	1177, 1909, 1915
Bst6I	CTCTTC	2 cut(s)	353, 434
BstACI	GRCGYC	1 cut(s)	826
BstAFI	CTTAAG	1 cut(s)	1499
BstAPI	GCANNNNNTGC	1 cut(s)	1412
BstBAI	YACGTR	2 cut(s)	312, 322
BstC8I	GCNNGC	3 cut(s)	293, 1205, 1719
BstDEI	CTNAG	8 cut(s)	172, 192, 252, 296, 1164, 1236, 1418, 1746
BstDSI	CCRYGG	4 cut(s)	183, 330, 377, 1798
BstEII	GGTNACC	1 cut(s)	1768
BstF5I	GGATG	7 cut(s)	229, 361, 892, 1072, 1246, 1438, 1516
BstFNI	CGCG	1 cut(s)	1800
BstH2I	RGCGCY	1 cut(s)	829
BstHHI	GCGC	1 cut(s)	828
BstMAI	GTCTC	2 cut(s)	166, 202
BstMCI	CGRYCG	1 cut(s)	1088
BstMWI	GCNNNNNNNGC	5 cut(s)	188, 1209, 1412, 1809, 1941
BstNI	CCWGG	2 cut(s)	1814, 1924
BstNSI	RCATGY	2 cut(s)	920, 986
BstPI	GGTNACC	1 cut(s)	1768
BstSCI	CCNGG	3 cut(s)	351, 1812, 1922
BstSFI	CTRYAG	1 cut(s)	734
BstSLI	GKGCMC	1 cut(s)	319
BstUI	CGCG	1 cut(s)	1800
BstV1I	GCAGC	1 cut(s)	720
BstV2I	GAAGAC	1 cut(s)	71
BstX2I	RGATCY	4 cut(s)	583, 942, 1300, 1919
BstYI	RGATCY	4 cut(s)	583, 942, 1300, 1919
BstZ17I	GTATAC	1 cut(s)	975
BsuI	GTATCC	1 cut(s)	1805
BsuRI	GGCC	9 cut(s)	29, 137, 182, 329, 637, 1212, 1366, 1615, 1985
BtgI	CCRYGG	4 cut(s)	183, 330, 377, 1798
BtgZI	GCGATG	1 cut(s)	624
BtsCI	GGATG	7 cut(s)	229, 361, 892, 1072, 1246, 1438, 1516
BtsI	GCAGTG	4 cut(s)	97, 183, 750, 1538
BtsIMutI	CAGTG	7 cut(s)	97, 110, 183, 750, 1538, 1719, 1905
Cac8I	GCNNGC	3 cut(s)	293, 1205, 1719
CciI	TCATGA	2 cut(s)	1141, 1620
CfoI	GCGC	1 cut(s)	828
Cfr10I	RCCGGY	1 cut(s)	869
Cfr13I	GGNCC	5 cut(s)	136, 374, 622, 635, 1484
Cfr42I	CCGCGG	1 cut(s)	1801
CseI	GACGC	2 cut(s)	1118, 1989
Csp6I	GTAC	1 cut(s)	1971
CviQI	GTAC	1 cut(s)	1971
DdeI	CTNAG	8 cut(s)	172, 192, 252, 296, 1164, 1236, 1418, 1746
DinI	GGCGCC	1 cut(s)	827
DriI	GACNNNNNGTC	1 cut(s)	372
EaeI	YGGCCR	4 cut(s)	27, 180, 327, 1983
Eam1104I	CTCTTC	2 cut(s)	353, 434
Eam1105I	GACNNNNNGTC	1 cut(s)	372
EarI	CTCTTC	2 cut(s)	353, 434
Eco130I	CCWWGG	4 cut(s)	330, 829, 1516, 1887
Eco24I	GRGCYC	1 cut(s)	133
Eco31I	GGTCTC	1 cut(s)	202
Eco47I	GGWCC	3 cut(s)	374, 622, 1484
Eco57I	CTGAAG	2 cut(s)	83, 1318
Eco72I	CACGTG	2 cut(s)	312, 322
Eco91I	GGTNACC	1 cut(s)	1768
EcoO109I	RGGNCCY	1 cut(s)	622
EcoO65I	GGTNACC	1 cut(s)	1768
EcoRI	GAATTC	2 cut(s)	300, 1145
EcoRII	CCWGG	2 cut(s)	1812, 1922
EcoT14I	CCWWGG	4 cut(s)	330, 829, 1516, 1887
EcoT38I	GRGCYC	1 cut(s)	133
EgeI	GGCGCC	1 cut(s)	827
EheI	GGCGCC	1 cut(s)	827
ErhI	CCWWGG	4 cut(s)	330, 829, 1516, 1887
FalI	AAGNNNNNCTT	2 cut(s)	249, 281
FaqI	GGGAC	1 cut(s)	635
FauI	CCCGC	1 cut(s)	1678
FauNDI	CATATG	1 cut(s)	1585
FbaI	TGATCA	1 cut(s)	1048
FblI	GTMKAC	1 cut(s)	974
Fnu4HI	GCNGC	4 cut(s)	148, 734, 1613, 1801
FokI	GGATG	7 cut(s)	236, 368, 879, 1079, 1253, 1445, 1523
FriOI	GRGCYC	1 cut(s)	133
Fsp4HI	GCNGC	4 cut(s)	148, 734, 1613, 1801
FspBI	CTAG	2 cut(s)	1262, 1298
GlaI	GCGC	1 cut(s)	827
GluI	GCNGC	4 cut(s)	148, 734, 1613, 1801
GsaI	CCCAGC	1 cut(s)	636
GsuI	CTGGAG	1 cut(s)	727
HaeII	RGCGCY	1 cut(s)	829
HaeIII	GGCC	9 cut(s)	29, 137, 182, 329, 637, 1212, 1366, 1615, 1985
HapII	CCGG	2 cut(s)	353, 870
HgaI	GACGC	2 cut(s)	1118, 1989
HhaI	GCGC	1 cut(s)	828
Hin1I	GRCGYC	1 cut(s)	826
Hin6I	GCGC	1 cut(s)	826
HinP1I	GCGC	1 cut(s)	826
HincII	GTYRAC	1 cut(s)	1591
HindII	GTYRAC	1 cut(s)	1591
HindIII	AAGCTT	1 cut(s)	1201
HinfI	GANTC	6 cut(s)	159, 278, 686, 1592, 1656, 1665
HpaII	CCGG	2 cut(s)	353, 870
HphI	GGTGA	3 cut(s)	725, 1411, 1829
Hpy166II	GTNNAC	5 cut(s)	317, 975, 1117, 1591, 1820
Hpy188I	TCNGA	8 cut(s)	15, 121, 286, 299, 1053, 1272, 1603, 1849
Hpy8I	GTNNAC	5 cut(s)	317, 975, 1117, 1591, 1820
HpyAV	CCTTC	8 cut(s)	52, 84, 214, 445, 772, 1457, 1515, 1835
HpyCH4III	ACNGT	3 cut(s)	1177, 1909, 1915
HpyCH4IV	ACGT	3 cut(s)	311, 321, 811
HpyF10VI	GCNNNNNNNGC	5 cut(s)	188, 1209, 1412, 1809, 1941
HpyF3I	CTNAG	8 cut(s)	172, 192, 252, 296, 1164, 1236, 1418, 1746
HpySE526I	ACGT	3 cut(s)	311, 321, 811
Hsp92I	GRCGYC	1 cut(s)	826
HspAI	GCGC	1 cut(s)	826
KasI	GGCGCC	1 cut(s)	825
Ksp22I	TGATCA	1 cut(s)	1048
KspI	CCGCGG	1 cut(s)	1801
LguI	GCTCTTC	1 cut(s)	353
LmnI	GCTCC	2 cut(s)	415, 1446
Lsp1109I	GCAGC	1 cut(s)	720
LweI	GCATC	9 cut(s)	214, 346, 474, 856, 901, 1234, 1414, 1501, 1842
MaeI	CTAG	2 cut(s)	1262, 1298
MaeII	ACGT	3 cut(s)	311, 321, 811
MaeIII	GTNAC	6 cut(s)	238, 364, 1130, 1735, 1768, 1909
MfeI	CAATTG	3 cut(s)	369, 773, 1953
MflI	RGATCY	4 cut(s)	583, 942, 1300, 1919
MhlI	GDGCHC	3 cut(s)	133, 319, 1538
MlsI	TGGCCA	3 cut(s)	29, 182, 329
MluNI	TGGCCA	3 cut(s)	29, 182, 329
Mly113I	GGCGCC	1 cut(s)	826
MlyI	GAGTC	5 cut(s)	168, 287, 695, 1586, 1674
MmeI	TCCRAC	3 cut(s)	293, 1360, 1424
Mox20I	TGGCCA	3 cut(s)	29, 182, 329
MroXI	GAANNNNTTC	1 cut(s)	1824
MscI	TGGCCA	3 cut(s)	29, 182, 329
MseI	TTAA	7 cut(s)	5, 93, 576, 663, 1500, 1557, 1868
MslI	CAYNNNNRTG	1 cut(s)	312
Msp20I	TGGCCA	3 cut(s)	29, 182, 329
MspA1I	CMGCKG	1 cut(s)	1800
MspCI	CTTAAG	1 cut(s)	1499
MspI	CCGG	2 cut(s)	353, 870
MspR9I	CCNGG	3 cut(s)	353, 1814, 1924
MunI	CAATTG	3 cut(s)	369, 773, 1953
MvaI	CCWGG	2 cut(s)	1814, 1924
MvnI	CGCG	1 cut(s)	1800
MwoI	GCNNNNNNNGC	5 cut(s)	188, 1209, 1412, 1809, 1941
NarI	GGCGCC	1 cut(s)	826
NciI	CCSGG	1 cut(s)	353
NcoI	CCATGG	1 cut(s)	330
NdeI	CATATG	1 cut(s)	1585
NlaIV	GGNNCC	7 cut(s)	417, 623, 827, 1174, 1474, 1885, 1945
NmuCI	GTSAC	2 cut(s)	364, 1130
NspI	RCATGY	2 cut(s)	920, 986
OliI	CACNNNNGTG	1 cut(s)	312
PagI	TCATGA	2 cut(s)	1141, 1620
PciI	ACATGT	1 cut(s)	982
PciSI	GCTCTTC	1 cut(s)	353
PdmI	GAANNNNTTC	1 cut(s)	1824
PfeI	GAWTC	1 cut(s)	1656
PflFI	GACNNNGTC	1 cut(s)	1964
PfoI	TCCNGGA	1 cut(s)	351
PkrI	GCNGC	4 cut(s)	149, 735, 1614, 1802
PleI	GAGTC	5 cut(s)	167, 286, 694, 1586, 1673
PluTI	GGCGCC	1 cut(s)	829
PmaCI	CACGTG	2 cut(s)	312, 322
PmlI	CACGTG	2 cut(s)	312, 322
PpsI	GAGTC	5 cut(s)	167, 286, 694, 1586, 1673
Ppu21I	YACGTR	2 cut(s)	312, 322
PpuMI	RGGWCCY	1 cut(s)	622
PscI	ACATGT	1 cut(s)	982
PshBI	ATTAAT	1 cut(s)	1868
PsiI	TTATAA	1 cut(s)	491
Psp5II	RGGWCCY	1 cut(s)	622
Psp6I	CCWGG	2 cut(s)	1812, 1922
PspCI	CACGTG	2 cut(s)	312, 322
PspEI	GGTNACC	1 cut(s)	1768
PspFI	CCCAGC	1 cut(s)	632
PspGI	CCWGG	2 cut(s)	1812, 1922
PspN4I	GGNNCC	7 cut(s)	417, 623, 827, 1174, 1474, 1885, 1945
PspPI	GGNCC	5 cut(s)	136, 374, 622, 635, 1484
PspPPI	RGGWCCY	1 cut(s)	622
PstI	CTGCAG	1 cut(s)	738
PsuI	RGATCY	4 cut(s)	583, 942, 1300, 1919
PsyI	GACNNNGTC	1 cut(s)	1964
RsaI	GTAC	1 cut(s)	1972
RsaNI	GTAC	1 cut(s)	1971
RseI	CAYNNNNRTG	1 cut(s)	312
SacII	CCGCGG	1 cut(s)	1801
SapI	GCTCTTC	1 cut(s)	353
SaqAI	TTAA	7 cut(s)	5, 93, 576, 663, 1500, 1557, 1868
SatI	GCNGC	4 cut(s)	148, 734, 1613, 1801
Sau96I	GGNCC	5 cut(s)	136, 374, 622, 635, 1484
SchI	GAGTC	5 cut(s)	168, 287, 695, 1586, 1674
ScrFI	CCNGG	3 cut(s)	353, 1814, 1924
SduI	GDGCHC	3 cut(s)	133, 319, 1538
SfaNI	GCATC	9 cut(s)	214, 346, 474, 856, 901, 1234, 1414, 1501, 1842
SfcI	CTRYAG	1 cut(s)	734
SfoI	GGCGCC	1 cut(s)	827
Sfr303I	CCGCGG	1 cut(s)	1801
SgrBI	CCGCGG	1 cut(s)	1801
SinI	GGWCC	3 cut(s)	374, 622, 1484
SmiMI	CAYNNNNRTG	1 cut(s)	312
SmlI	CTYRAG	2 cut(s)	683, 1499
SmoI	CTYRAG	2 cut(s)	683, 1499
SsiI	CCGC	6 cut(s)	147, 800, 1612, 1685, 1798, 1800
SspDI	GGCGCC	1 cut(s)	825
SspMI	CTAG	2 cut(s)	1262, 1298
StyD4I	CCNGG	3 cut(s)	351, 1812, 1922
StyI	CCWWGG	4 cut(s)	330, 829, 1516, 1887
TaaI	ACNGT	3 cut(s)	1177, 1909, 1915
TaiI	ACGT	3 cut(s)	314, 324, 814
TaqI	TCGA	5 cut(s)	200, 304, 678, 1084, 1156
TaqII	GACCGA	1 cut(s)	1102
TatI	WGTACW	1 cut(s)	1970
TauI	GCSGC	3 cut(s)	150, 1615, 1803
TfiI	GAWTC	1 cut(s)	1656
Tru1I	TTAA	7 cut(s)	5, 93, 576, 663, 1500, 1557, 1868
Tru9I	TTAA	7 cut(s)	5, 93, 576, 663, 1500, 1557, 1868
TscAI	CASTG	7 cut(s)	104, 117, 183, 757, 1538, 1719, 1912
TseFI	GTSAC	2 cut(s)	364, 1130
TseI	GCWGC	1 cut(s)	733
Tsp45I	GTSAC	2 cut(s)	364, 1130
TspGWI	ACGGA	3 cut(s)	200, 366, 440
TspRI	CASTG	7 cut(s)	104, 117, 183, 757, 1538, 1719, 1912
Tth111I	GACNNNGTC	1 cut(s)	1964
Vha464I	CTTAAG	1 cut(s)	1499
VneI	GTGCAC	1 cut(s)	315
VpaK11BI	GGWCC	3 cut(s)	374, 622, 1484
VspI	ATTAAT	1 cut(s)	1868
XapI	RAATTY	6 cut(s)	300, 478, 561, 1145, 1843, 1860
XbaI	TCTAGA	1 cut(s)	1261
XceI	RCATGY	2 cut(s)	920, 986
XcmI	CCANNNNNNNNNTGG	1 cut(s)	953
XmiI	GTMKAC	1 cut(s)	974
XmnI	GAANNNNTTC	1 cut(s)	1824
XspI	CTAG	2 cut(s)	1262, 1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.