FvH4_6g49510

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0018068
Physical Location & Seq
Forward (+)
37066830 .. 37069448
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49510.t1

Sequence Viewer

Length: 1530 bp
ATGGCCTCATACACAACAATACATGCATCGTCGTTTTCCTTCTTTTCGTCAGCAACAAGGCGCAGGCACGACGTGTTCTTAAGCTTCAGAGGCGAGGACACCCGCCACAACATCACCAAAGGCCTCCACAGCGCCCTAGTTGACGCCGGCGTTCCCACATTCAAAGACGATGTCAATCTTCAAAAGGGCTGCGAGATCATGTCTGATCTGCTCAAGGCCATACAGATGTCAAGAATCGCCATCATAGTGTTCTCCAAGAACTACGCGAGCTCAGGGTGGTGTCTGGATGAGTTGGTCAAGATCGTAGAGTGTAACAAGGACGCCGGTCAGCTCATAATGCCTATTTTCTGCGATGTGGATCCTTGCGATGTGCAGCAGCAGACGGGTACATTTTCAGCAGCACTTGATCAGCATGAAAGGACTGGCAGGACTGAGGACAAGTTGGAGGCTTGGAGGACATCGTTGACTGAAGCGGCTAGTCAACCTTATGGCTTTGATATGCAGGATGCTGCTGCTTGGCTTCAATCAAAGTTCATCAAGGAAATTGTAAAACATGTCTTGAGTAGATATCAAGACGCACACTTGAGTGTTTCCATTTTCCCACCCAGATTGGATGCTGGTAAAGAATACATGAATTCTTTGTTAGGTGTAATATCAGATGACATTCTCATAATAGGCATCTGTGGAATCGACAAAACTGATTTGGCACAAGAAGCGTATGACCAGAATTTTCATAGGTTTGAAAGCAGCAGTTTTATTAAAGATGTGAACAAGATGGCAGAACAACCCAATGGACTAGTTGCCTTGCAAAAGAGGTTCCTTTCAGATATGCTGGAAGTGGACAATGTAGACATAAATGATGTTAAGGAAGGAACTGAGTTGATCAAATATGAAGCACGAAGAAAAAGAGTGCTTTTCATTCTTGACAATCTGAATAAGCTAGACCAGTTGAATGCACTTGCTAGGAGCCGGGATTGGTTTGGCTCAGGAAGCAGAATAGTGATAACTACTAGAGATCCACAGCTGCTAAGTGTACTTGAAGTTGATAATGTATATGGCGAACAAGGCATAGAGGTACTTGAAACACAGTTTCAGCAGAACCTTCAGATGATTAAAGATATGGAGGAGACTATCAGAGGGCCATCCTTTCAGGCTCATGATCTAGAGAGGTCCCTTATCGCTAAAATAGAAGCAGAACGGAGAAAGAACAATGCTCAGGCGCAAATTATAGCAGAGTTGCAGAAAAATTCGAAATCAAGTTTCCCCTTGTATGCATTAGACTCAACAACAGATGTTCTACTAGATGGAAAAGGGCCTGAAACTTTTGAAGGATATGATGATCACGAAGCCCTGCTCAGTATATTTGCTGAGAATGAGATGATAATTGGGGATGCAAATGTAAATGATCAGTTGAAGGCAAAGATGCAATTTCTGAATGTTGGAGCCAAGGATTTCAATAAGGCAGTAAGCAGTGGTTCATCCAGCAAAGGCATTAATTCAGGGAGGGAAGCAGGAACGTTTGTAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

510

Amino Acids

57.0

Weight (kDa)

5.07

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 191 2e-46 TIR domain
TIR_2 PF13676 25 - 124 9.9e-13 TIR domain
NB-ARC PF00931 222 - 344 6.5e-07 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49510
rosa_laevigata RLG00000021862
rosa_multiflora Rmu_sc0000648.1_g000014 Rmu_sc0035653.1_g000001
rosa_roxburghii Rroxscaffold_2G00082140 Rroxscaffold_2G00082170
rosa_rugosa Rorug02G0539900 Rorug02G0539900
rosa_samantha Rh2AG611200 Rh2BG624800
rosa_wichuraiana Rw2G050720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 849
AccII CGCG 1 cut(s) 266
AciI CCGC 2 cut(s) 103, 473
AclI AACGTT 1 cut(s) 1517
AclWI GGATC 3 cut(s) 353, 366, 1010
AcsI RAATTY 3 cut(s) 634, 727, 1246
AcuI CTGAAG 3 cut(s) 70, 489, 1088
AcyI GRCGYC 2 cut(s) 144, 321
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 3 cut(s) 388, 1035, 1077
AflII CTTAAG 1 cut(s) 79
AflIII ACRYGT 2 cut(s) 72, 553
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 73
AleI CACNNNNGTG 1 cut(s) 585
AluBI AGCT 5 cut(s) 84, 270, 331, 940, 1024
AluI AGCT 5 cut(s) 84, 270, 331, 940, 1024
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 1121
AlwI GGATC 3 cut(s) 353, 366, 1010
AoxI GGCC 5 cut(s) 3, 121, 216, 1139, 1313
ApeKI GCWGC 8 cut(s) 189, 373, 376, 398, 509, 512, 747, 1024
ApoI RAATTY 3 cut(s) 634, 727, 1246
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AseI ATTAAT 1 cut(s) 1494
AspLEI GCGC 3 cut(s) 63, 134, 1222
AspS9I GGNCC 3 cut(s) 1139, 1170, 1313
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 1250
AvaII GGWCC 1 cut(s) 1170
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 8 cut(s) 176, 385, 388, 410, 496, 499, 759, 1011
BccI CCATC 4 cut(s) 248, 769, 1150, 1298
BclI TGATCA 4 cut(s) 406, 882, 1339, 1405
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 1 cut(s) 1121
BcuI ACTAGT 1 cut(s) 796
BfaI CTAG 8 cut(s) 137, 477, 797, 941, 963, 1011, 1163, 1301
BfoI RGCGCY 1 cut(s) 135
BfrI CTTAAG 1 cut(s) 79
BisI GCNGC 9 cut(s) 190, 374, 377, 399, 474, 510, 513, 748, 1025
BlsI GCNGC 9 cut(s) 191, 375, 378, 400, 475, 511, 514, 749, 1026
Bme1390I CCNGG 1 cut(s) 971
Bme18I GGWCC 1 cut(s) 1170
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 1139, 1170, 1313
BmiI GGNNCC 5 cut(s) 360, 818, 968, 1172, 1444
BmrFI CCNGG 1 cut(s) 971
BmsI GCATC 6 cut(s) 35, 496, 604, 687, 1381, 1413
BoxI GACNNNNGTC 1 cut(s) 324
Bpu10I CCTNAGC 3 cut(s) 271, 985, 1215
Bpu14I TTCGAA 1 cut(s) 1250
BpuEI CTTGAG 3 cut(s) 197, 580, 604
BpuMI CCSGG 1 cut(s) 971
BsaBI GATNNNNATC 2 cut(s) 174, 357
BsaHI GRCGYC 2 cut(s) 144, 321
BsaJI CCNNGG 1 cut(s) 1446
Bse118I RCCGGY 2 cut(s) 146, 323
Bse1I ACTGG 2 cut(s) 427, 946
Bse8I GATNNNNATC 2 cut(s) 174, 357
BseDI CCNNGG 1 cut(s) 1446
BseGI GGATG 6 cut(s) 292, 511, 619, 1142, 1396, 1478
BseJI GATNNNNATC 2 cut(s) 174, 357
BseMII CTCAG 7 cut(s) 285, 423, 867, 999, 1229, 1359, 1369
BseNI ACTGG 2 cut(s) 427, 946
BseRI GAGGAG 1 cut(s) 1139
BseXI GCAGC 8 cut(s) 176, 385, 388, 410, 496, 499, 759, 1011
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 5 cut(s) 5, 123, 218, 1141, 1315
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 3 cut(s) 147, 324, 970
BslFI GGGAC 1 cut(s) 1156
BsmAI GTCTC 1 cut(s) 1121
BsmFI GGGAC 1 cut(s) 1156
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 5 cut(s) 5, 123, 218, 1141, 1315
Bsp119I TTCGAA 1 cut(s) 1250
Bsp1286I GDGCHC 1 cut(s) 272
BspACI CCGC 2 cut(s) 103, 473
BspANI GGCC 5 cut(s) 5, 123, 218, 1141, 1315
BspCNI CTCAG 7 cut(s) 284, 424, 868, 998, 1228, 1360, 1368
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 1156
BspLI GGNNCC 5 cut(s) 360, 818, 968, 1172, 1444
BspPI GGATC 3 cut(s) 353, 366, 1010
BspT104I TTCGAA 1 cut(s) 1250
BspTI CTTAAG 1 cut(s) 79
BsrFI RCCGGY 2 cut(s) 146, 323
BsrI ACTGG 2 cut(s) 427, 946
BssAI RCCGGY 2 cut(s) 146, 323
BssECI CCNNGG 1 cut(s) 1446
BssNI GRCGYC 2 cut(s) 144, 321
BssT1I CCWWGG 1 cut(s) 1446
Bst4CI ACNGT 1 cut(s) 1089
BstACI GRCGYC 2 cut(s) 144, 321
BstAFI CTTAAG 1 cut(s) 79
BstBI TTCGAA 1 cut(s) 1250
BstC8I GCNNGC 3 cut(s) 65, 148, 268
BstDEI CTNAG 8 cut(s) 271, 432, 876, 985, 1028, 1215, 1355, 1368
BstF5I GGATG 6 cut(s) 292, 511, 619, 1142, 1396, 1478
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 3 cut(s) 63, 134, 1222
BstMAI GTCTC 1 cut(s) 1121
BstMWI GCNNNNNNNGC 6 cut(s) 90, 129, 337, 713, 990, 1065
BstNSI RCATGY 2 cut(s) 26, 557
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 969
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 8 cut(s) 176, 385, 388, 410, 496, 499, 759, 1011
BstX2I RGATCY 2 cut(s) 358, 1015
BstYI RGATCY 2 cut(s) 358, 1015
BsuRI GGCC 5 cut(s) 5, 123, 218, 1141, 1315
BtgZI GCGATG 2 cut(s) 366, 381
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 6 cut(s) 292, 511, 619, 1142, 1396, 1478
BtsI GCAGTG 1 cut(s) 1477
BtsIMutI CAGTG 1 cut(s) 1477
Cac8I GCNNGC 3 cut(s) 65, 148, 268
CciI TCATGA 1 cut(s) 1156
CfoI GCGC 3 cut(s) 63, 134, 1222
Cfr10I RCCGGY 2 cut(s) 146, 323
Cfr13I GGNCC 3 cut(s) 1139, 1170, 1313
CseI GACGC 3 cut(s) 152, 329, 584
Csp6I GTAC 3 cut(s) 387, 1034, 1076
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 6 cut(s) 23, 199, 413, 554, 631, 1157
CviQI GTAC 3 cut(s) 387, 1034, 1076
DdeI CTNAG 8 cut(s) 271, 432, 876, 985, 1028, 1215, 1355, 1368
DraIII CACNNNGTG 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 1446
Eco147I AGGCCT 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 272
Eco32I GATATC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 1170
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 3 cut(s) 70, 489, 1088
EcoICRI GAGCTC 1 cut(s) 270
EcoO109I RGGNCCY 2 cut(s) 1170, 1313
EcoRI GAATTC 1 cut(s) 634
EcoRV GATATC 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 1446
EcoT22I ATGCAT 2 cut(s) 28, 1276
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 1446
FaeI CATG 6 cut(s) 26, 202, 416, 557, 634, 1160
FaqI GGGAC 1 cut(s) 1156
FatI CATG 6 cut(s) 22, 198, 412, 553, 630, 1156
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 4 cut(s) 406, 882, 1339, 1405
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 9 cut(s) 190, 374, 377, 399, 474, 510, 513, 748, 1025
FokI GGATG 6 cut(s) 299, 518, 626, 1129, 1403, 1465
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 9 cut(s) 190, 374, 377, 399, 474, 510, 513, 748, 1025
FspBI CTAG 8 cut(s) 137, 477, 797, 941, 963, 1011, 1163, 1301
GlaI GCGC 3 cut(s) 62, 133, 1221
GluI GCNGC 9 cut(s) 190, 374, 377, 399, 474, 510, 513, 748, 1025
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 5 cut(s) 5, 123, 218, 1141, 1315
HapII CCGG 3 cut(s) 147, 324, 970
HgaI GACGC 3 cut(s) 152, 329, 584
HhaI GCGC 3 cut(s) 63, 134, 1222
Hin1I GRCGYC 2 cut(s) 144, 321
Hin1II CATG 6 cut(s) 26, 202, 416, 557, 634, 1160
Hin6I GCGC 3 cut(s) 61, 132, 1220
HinP1I GCGC 3 cut(s) 61, 132, 1220
HincII GTYRAC 3 cut(s) 142, 465, 482
HindII GTYRAC 3 cut(s) 142, 465, 482
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 3 cut(s) 234, 687, 1280
HpaII CCGG 3 cut(s) 147, 324, 970
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 7 cut(s) 142, 465, 482, 769, 841, 850, 1034
Hpy188I TCNGA 8 cut(s) 89, 205, 658, 826, 933, 1107, 1136, 1434
Hpy8I GTNNAC 7 cut(s) 142, 465, 482, 769, 841, 850, 1034
Hpy99I CGWCG 2 cut(s) 34, 74
HpyAV CCTTC 5 cut(s) 49, 863, 1112, 1322, 1408
HpyCH4III ACNGT 1 cut(s) 1089
HpyCH4IV ACGT 2 cut(s) 72, 1517
HpyCH4V TGCA 9 cut(s) 26, 373, 502, 808, 956, 1240, 1274, 1394, 1426
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 129, 337, 713, 990, 1065
HpyF3I CTNAG 8 cut(s) 271, 432, 876, 985, 1028, 1215, 1355, 1368
HpySE526I ACGT 2 cut(s) 72, 1517
Hsp92I GRCGYC 2 cut(s) 144, 321
Hsp92II CATG 6 cut(s) 26, 202, 416, 557, 634, 1160
HspAI GCGC 3 cut(s) 61, 132, 1220
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 4 cut(s) 406, 882, 1339, 1405
LmnI GCTCC 2 cut(s) 966, 1442
Lsp1109I GCAGC 8 cut(s) 176, 385, 388, 410, 496, 499, 759, 1011
LweI GCATC 6 cut(s) 35, 496, 604, 687, 1381, 1413
MaeI CTAG 8 cut(s) 137, 477, 797, 941, 963, 1011, 1163, 1301
MaeII ACGT 2 cut(s) 72, 1517
MaeIII GTNAC 1 cut(s) 311
MboII GAAGA 2 cut(s) 170, 912
MflI RGATCY 2 cut(s) 358, 1015
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 8 cut(s) 543, 634, 727, 1224, 1246, 1383, 1427, 1495
MlyI GAGTC 1 cut(s) 1274
MmeI TCCRAC 2 cut(s) 423, 1420
Mph1103I ATGCAT 2 cut(s) 28, 1276
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MseI TTAA 5 cut(s) 80, 759, 864, 1113, 1494
MslI CAYNNNNRTG 3 cut(s) 224, 245, 585
MspA1I CMGCKG 1 cut(s) 1024
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 3 cut(s) 147, 324, 970
MspR9I CCNGG 1 cut(s) 971
Mva1269I GAATGC 1 cut(s) 958
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 6 cut(s) 90, 129, 337, 713, 990, 1065
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 971
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 6 cut(s) 26, 202, 416, 557, 634, 1160
NlaIV GGNNCC 5 cut(s) 360, 818, 968, 1172, 1444
NsiI ATGCAT 2 cut(s) 28, 1276
NspI RCATGY 2 cut(s) 26, 557
NspV TTCGAA 1 cut(s) 1250
OliI CACNNNNGTG 1 cut(s) 585
PagI TCATGA 1 cut(s) 1156
PceI AGGCCT 1 cut(s) 123
PciI ACATGT 1 cut(s) 553
PctI GAATGC 1 cut(s) 958
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 234, 687
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 9 cut(s) 191, 375, 378, 400, 475, 511, 514, 749, 1026
PleI GAGTC 1 cut(s) 1274
PpsI GAGTC 1 cut(s) 1274
PpuMI RGGWCCY 1 cut(s) 1170
PscI ACATGT 1 cut(s) 553
PshAI GACNNNNGTC 1 cut(s) 324
PshBI ATTAAT 1 cut(s) 1494
Psp124BI GAGCTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 1517
Psp5II RGGWCCY 1 cut(s) 1170
PspN4I GGNNCC 5 cut(s) 360, 818, 968, 1172, 1444
PspPI GGNCC 3 cut(s) 1139, 1170, 1313
PspPPI RGGWCCY 1 cut(s) 1170
PsuI RGATCY 2 cut(s) 358, 1015
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 3 cut(s) 388, 1035, 1077
RsaNI GTAC 3 cut(s) 387, 1034, 1076
RseI CAYNNNNRTG 3 cut(s) 224, 245, 585
SacI GAGCTC 1 cut(s) 272
SaqAI TTAA 5 cut(s) 80, 759, 864, 1113, 1494
SatI GCNGC 9 cut(s) 190, 374, 377, 399, 474, 510, 513, 748, 1025
Sau96I GGNCC 3 cut(s) 1139, 1170, 1313
SchI GAGTC 1 cut(s) 1274
ScrFI CCNGG 1 cut(s) 971
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 6 cut(s) 35, 496, 604, 687, 1381, 1413
SfuI TTCGAA 1 cut(s) 1250
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 1170
SmiMI CAYNNNNRTG 3 cut(s) 224, 245, 585
SmlI CTYRAG 4 cut(s) 79, 212, 559, 583
SmoI CTYRAG 4 cut(s) 79, 212, 559, 583
SpeI ACTAGT 1 cut(s) 796
Sse9I AATT 8 cut(s) 543, 634, 727, 1224, 1246, 1383, 1427, 1495
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 2 cut(s) 103, 473
SspMI CTAG 8 cut(s) 137, 477, 797, 941, 963, 1011, 1163, 1301
SstI GAGCTC 1 cut(s) 272
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 969
StyI CCWWGG 1 cut(s) 1446
TaaI ACNGT 1 cut(s) 1089
TaiI ACGT 2 cut(s) 75, 1520
TaqI TCGA 2 cut(s) 690, 1250
TasI AATT 8 cut(s) 543, 634, 727, 1224, 1246, 1383, 1427, 1495
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 2 cut(s) 234, 687
Tru1I TTAA 5 cut(s) 80, 759, 864, 1113, 1494
Tru9I TTAA 5 cut(s) 80, 759, 864, 1113, 1494
TscAI CASTG 1 cut(s) 1477
TseI GCWGC 8 cut(s) 189, 373, 376, 398, 509, 512, 747, 1024
TspDTI ATGAA 7 cut(s) 429, 523, 647, 722, 906, 907, 1467
TspGWI ACGGA 1 cut(s) 1213
TspRI CASTG 1 cut(s) 1477
Tth111I GACNNNGTC 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 1170
VspI ATTAAT 1 cut(s) 1494
XapI RAATTY 3 cut(s) 634, 727, 1246
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 2 cut(s) 26, 557
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 8 cut(s) 137, 477, 797, 941, 963, 1011, 1163, 1301
Zsp2I ATGCAT 2 cut(s) 28, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.